##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-9A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 383954 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.26227621017101 40.0 40.0 40.0 40.0 40.0 2 39.16150372180001 40.0 40.0 40.0 40.0 40.0 3 39.14736661162535 40.0 40.0 40.0 40.0 40.0 4 39.06575266828839 40.0 40.0 40.0 40.0 40.0 5 39.21268693645593 40.0 40.0 40.0 40.0 40.0 6 39.26450564390526 40.0 40.0 40.0 40.0 40.0 7 39.29693661219834 40.0 40.0 40.0 40.0 40.0 8 39.314511634206184 40.0 40.0 40.0 40.0 40.0 9 39.33014892408986 40.0 40.0 40.0 40.0 40.0 10-11 39.31709527703839 40.0 40.0 40.0 40.0 40.0 12-13 39.31860587466207 40.0 40.0 40.0 40.0 40.0 14-15 39.321976070050056 40.0 40.0 40.0 40.0 40.0 16-17 39.46163863379468 40.0 40.0 40.0 40.0 40.0 18-19 39.47727592367836 40.0 40.0 40.0 40.0 40.0 20-21 39.48684477828073 40.0 40.0 40.0 40.0 40.0 22-23 39.491918302713344 40.0 40.0 40.0 40.0 40.0 24-25 39.48598009136511 40.0 40.0 40.0 40.0 40.0 26-27 39.47916156622929 40.0 40.0 40.0 40.0 40.0 28-29 39.47813540163665 40.0 40.0 40.0 40.0 40.0 30-31 39.466126947940765 40.0 40.0 40.0 40.0 40.0 32-33 39.47023781648823 40.0 40.0 40.0 40.0 40.0 34-35 39.46726334684522 40.0 40.0 40.0 40.0 40.0 36-37 39.46811604502033 40.0 40.0 40.0 40.0 40.0 38-39 39.447362027697636 40.0 40.0 40.0 40.0 40.0 40-41 39.43789544085432 40.0 40.0 40.0 40.0 40.0 42-43 39.436509030461075 40.0 40.0 40.0 40.0 40.0 44-45 39.42418144049863 40.0 40.0 40.0 40.0 40.0 46-47 39.41093330325174 40.0 40.0 40.0 40.0 40.0 48-49 39.42538280094567 40.0 40.0 40.0 40.0 40.0 50-51 39.411037989264614 40.0 40.0 40.0 40.0 40.0 52-53 39.39537684478914 40.0 40.0 40.0 40.0 40.0 54-55 39.38444485735024 40.0 40.0 40.0 40.0 40.0 56-57 39.37520036596293 40.0 40.0 40.0 40.0 40.0 58-59 39.376717325460405 40.0 40.0 40.0 40.0 40.0 60-61 39.33755474349228 40.0 40.0 40.0 40.0 40.0 62-63 39.35715203853522 40.0 40.0 40.0 40.0 40.0 64-65 39.35516851602692 40.0 40.0 40.0 40.0 40.0 66-67 39.34402120744882 40.0 40.0 40.0 40.0 40.0 68-69 39.348839379750146 40.0 40.0 40.0 40.0 40.0 70-71 39.353387522599284 40.0 40.0 40.0 40.0 40.0 72-73 39.37542394994932 40.0 40.0 40.0 40.0 40.0 74-75 39.361275726258405 40.0 40.0 40.0 40.0 40.0 76-77 39.35307898030682 40.0 40.0 40.0 40.0 40.0 78-79 39.34120605843624 40.0 40.0 40.0 40.0 40.0 80-81 39.303124465666805 40.0 40.0 40.0 40.0 40.0 82-83 39.32646596996876 40.0 40.0 40.0 40.0 40.0 84-85 39.320507853049605 40.0 40.0 40.0 40.0 40.0 86-87 39.312292664420454 40.0 40.0 40.0 40.0 40.0 88-89 39.365272405378136 40.0 40.0 40.0 40.0 40.0 90-91 39.39929616070101 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18244739051305459 1101 2 0.048879283687071506 1101 3 0.09464525753489994 1101 4 0.03946089345343751 1101 5 -0.11131021622344406 1101 6 0.030794034964657158 1101 7 0.10280824160683011 1101 8 -0.11210204735300522 1101 9 0.19513144651864422 1101 10-11 -0.05211095330665927 1101 12-13 0.04324058605579495 1101 14-15 -0.0699597805474852 1101 16-17 -0.01891089792872691 1101 18-19 0.04711283906318897 1101 20-21 0.0666087905272903 1101 22-23 0.08356367702691614 1101 24-25 0.01571506455448457 1101 26-27 0.05063843388745681 1101 28-29 0.01893759946901241 1101 30-31 0.09358864161261238 1101 32-33 0.03949675406813924 1101 34-35 0.03169777204990254 1101 36-37 -0.027417085987352152 1101 38-39 0.06686922754467872 1101 40-41 0.024670134556188827 1101 42-43 0.12917522091739642 1101 44-45 0.06683687488443724 1101 46-47 -0.1234791863490372 1101 48-49 -0.10691381555405854 1101 50-51 -0.024545887314147308 1101 52-53 0.05258130179584697 1101 54-55 -0.01859570654836773 1101 56-57 0.06249386509689714 1101 58-59 0.050172749178855724 1101 60-61 -0.13625432946966498 1101 62-63 0.25076773781952255 1101 64-65 -0.06625538678308374 1101 66-67 0.041628673647302605 1101 68-69 -0.06785823103901834 1101 70-71 0.317976804428433 1101 72-73 -0.08735111706045018 1101 74-75 -0.11216223888148136 1101 76-77 -0.19513705311120333 1101 78-79 0.2826173003961827 1101 80-81 0.5875992819939526 1101 82-83 0.32536419049476706 1101 84-85 0.11188246048797623 1101 86-87 0.3439359188826714 1101 88-89 0.6091991617371093 1101 90-91 0.5532991314241329 1102 1 0.21845367267504656 1102 2 0.18537995785799666 1102 3 0.32038899178530755 1102 4 0.15036832246348553 1102 5 -0.05856833531182559 1102 6 0.1129166381229112 1102 7 0.02280662561660307 1102 8 0.10981381005605328 1102 9 0.06490283440174238 1102 10-11 -0.06574082084596 1102 12-13 -4.214497888952451E-4 1102 14-15 0.07296950473034514 1102 16-17 -4.7850944467597856E-4 1102 18-19 -0.10971901210411517 1102 20-21 -0.023432164724923155 1102 22-23 0.020226960214053236 1102 24-25 0.08713173248364114 1102 26-27 0.021202161976901834 1102 28-29 0.014433026721683007 1102 30-31 -0.08581657298584844 1102 32-33 0.013768550134464874 1102 34-35 -0.05939927181014326 1102 36-37 -0.017584704207820323 1102 38-39 -0.05728224986955155 1102 40-41 0.12314362314706528 1102 42-43 -0.11283169564282503 1102 44-45 0.012901225033026265 1102 46-47 -0.14347117944038956 1102 48-49 0.02994160166770854 1102 50-51 -0.24333324633687425 1102 52-53 -0.1471586301850607 1102 54-55 -0.034096353668971346 1102 56-57 -0.16325292510706646 1102 58-59 -0.08558880512630651 1102 60-61 -0.29676356963941686 1102 62-63 -0.41439101699440783 1102 64-65 0.05227202069850989 1102 66-67 -0.15717615160692588 1102 68-69 0.010304133016710182 1102 70-71 -0.1518775950969058 1102 72-73 0.20149366986618134 1102 74-75 -0.2209234890288556 1102 76-77 -0.3849756778037232 1102 78-79 0.043150806292068467 1102 80-81 0.409467012136858 1102 82-83 0.4809197460503185 1102 84-85 -0.5547842061786952 1102 86-87 -0.16483601094189027 1102 88-89 0.10919916173710931 1102 90-91 0.5532991314241329 1103 1 0.2611952936753994 1103 2 -0.07790432266660474 1103 3 -0.17390528401872984 1103 4 0.04345782844628587 1103 5 0.1403931407877579 1103 6 -0.1131059795320013 1103 7 0.047948755616808114 1103 8 0.09983905480699917 1103 9 -0.02764072818676766 1103 10-11 0.15410914734155057 1103 12-13 0.003457621696860258 1103 14-15 0.09275482172642313 1103 16-17 0.15329383626473714 1103 18-19 0.06473145276873993 1103 20-21 0.12838525210080576 1103 22-23 0.11577731630871568 1103 24-25 0.09744513683065748 1103 26-27 0.04324513962606602 1103 28-29 0.05912957660571294 1103 30-31 -0.0054028053607027005 1103 32-33 0.06793465750774175 1103 34-35 0.1725634898080486 1103 36-37 0.034420487233845165 1103 38-39 0.12715064739446547 1103 40-41 0.03655412449474227 1103 42-43 0.07859895215491974 1103 44-45 0.006214031461027503 1103 46-47 0.10457235583675839 1103 48-49 0.04425821930224316 1103 50-51 0.37471867611678533 1103 52-53 -0.03454764753081463 1103 54-55 0.11165505079767968 1103 56-57 0.1803588159520686 1103 58-59 0.011320626220623353 1103 60-61 -0.37219345334990805 1103 62-63 -0.18993450288271418 1103 64-65 -0.04337367682267512 1103 66-67 0.1359654429978292 1103 68-69 0.0811584741301985 1103 70-71 -0.013716226066051718 1103 72-73 0.14774243964436096 1103 74-75 -0.33654964357270245 1103 76-77 -0.12919987465949845 1103 78-79 0.23183206827054903 1103 80-81 -0.26217844855526096 1103 82-83 0.36707359220416436 1103 84-85 -0.10963555637887623 1103 86-87 -0.3932205924673937 1103 88-89 -0.2719602585527525 1103 90-91 -0.33558975746475284 1104 1 0.3245704747473468 1104 2 0.24142611351559395 1104 3 0.34782334949165516 1104 4 0.13512047129193405 1104 5 0.2387353265273333 1104 6 0.2255387975483174 1104 7 0.04859256698007641 1104 8 0.19138098302202877 1104 9 0.10699632315719043 1104 10-11 0.08045310356236968 1104 12-13 0.05655194006157416 1104 14-15 0.018615919424796346 1104 16-17 0.005820795865574269 1104 18-19 0.0014166290121053748 1104 20-21 -0.02178374838205599 1104 22-23 0.007878555645582708 1104 24-25 -0.049524925940943376 1104 26-27 0.019906977707506712 1104 28-29 0.09669194583153029 1104 30-31 -9.967213433128563E-4 1104 32-33 0.026070418184609423 1104 34-35 -0.13315512974366328 1104 36-37 -0.09732701660747978 1104 38-39 0.08847315616082341 1104 40-41 -0.055515543569981673 1104 42-43 -0.04804558478710419 1104 44-45 -0.14020686226971435 1104 46-47 0.10765561469799678 1104 48-49 -0.3228653062251752 1104 50-51 -0.14782003430016744 1104 52-53 -0.04641973576650571 1104 54-55 -0.17517514139075985 1104 56-57 0.1505523994761475 1104 58-59 -0.09073711292627706 1104 60-61 0.06179860318253816 1104 62-63 0.05946294184287382 1104 64-65 -0.25663471371413493 1104 66-67 -0.17677194154630627 1104 68-69 0.19248251780318526 1104 70-71 0.1790506242638159 1104 72-73 0.14209873672662354 1104 74-75 0.4996099770897189 1104 76-77 -0.14563007057041943 1104 78-79 -0.16798119036624115 1104 80-81 0.2851643333188463 1104 82-83 0.05527872040929793 1104 84-85 0.11383992331499826 1104 86-87 0.13686521181195843 1104 88-89 0.033441585979531396 1104 90-91 0.5532991314241329 1201 1 0.3856366016029469 1201 2 0.24300056245846235 1201 3 0.3343280728384812 1201 4 0.10857976054069951 1201 5 0.1971248901800564 1201 6 0.2208154507197051 1201 7 0.15408064615236583 1201 8 0.1314394625541908 1201 9 0.13353703390842497 1201 10-11 0.3221872466123088 1201 12-13 -0.021889355484979944 1201 14-15 0.02204596890747723 1201 16-17 -0.15567268141737145 1201 18-19 0.06175176171565511 1201 20-21 0.015215801775390503 1201 22-23 0.039873607377479914 1201 24-25 0.11404907315937152 1201 26-27 -0.052967516219624144 1201 28-29 0.07324390264664515 1201 30-31 0.10488497006468833 1201 32-33 0.11426320974732818 1201 34-35 0.026413748775958368 1201 36-37 -0.02049270308953055 1201 38-39 0.037435774933555876 1201 40-41 0.0586695056390667 1201 42-43 0.09136209372049109 1201 44-45 0.1475183661379802 1201 46-47 -0.022537110626153378 1201 48-49 0.08715512140194903 1201 50-51 0.21997467551022964 1201 52-53 -0.18184198055406853 1201 54-55 0.12897634787512402 1201 56-57 0.3317546026860825 1201 58-59 0.2215848802423679 1201 60-61 0.08140060394432425 1201 62-63 0.1717842767465143 1201 64-65 -0.20854863067519602 1201 66-67 -0.0660798273145673 1201 68-69 -0.011260386983359183 1201 70-71 -0.3687288558080297 1201 72-73 -0.05260144644121567 1201 74-75 0.06740594343186501 1201 76-77 -0.410503880943871 1201 78-79 -0.022871103320667885 1201 80-81 -0.09877719463676016 1201 82-83 -0.3985233333927596 1201 84-85 -1.3959850343360358 1201 86-87 -0.9250034750567266 1201 88-89 -4.501911949374005 1201 90-91 -3.732415154290152 1202 1 0.15152757337627065 1202 2 0.1515691180858454 1202 3 -0.023030255108082542 1202 4 0.0888282702009704 1202 5 0.013687412624932449 1202 6 0.023120988319362823 1202 7 0.13321588999349387 1202 8 0.048339555924876265 1202 9 0.21351261388400644 1202 10-11 0.029793365983131537 1202 12-13 0.05266354259732253 1202 14-15 0.05574919378717169 1202 16-17 -0.013713333575680053 1202 18-19 -0.03136753728599473 1202 20-21 -0.04588812697560485 1202 22-23 -0.19389425874332744 1202 24-25 -0.06649750444603342 1202 26-27 -0.02878091850361386 1202 28-29 -0.12310386723053313 1202 30-31 0.14990794568703336 1202 32-33 -0.1550262018076296 1202 34-35 -0.08688794894094087 1202 36-37 -0.10979762331917442 1202 38-39 0.030203221510795686 1202 40-41 -0.07371939621856427 1202 42-43 -0.052125156146246354 1202 44-45 6.668437036410069E-4 1202 46-47 -0.02192055214739952 1202 48-49 0.041444793187004336 1202 50-51 -0.03465984830198465 1202 52-53 -0.1359061902095391 1202 54-55 -0.010674449408099917 1202 56-57 0.10049367954636068 1202 58-59 0.028810404163863268 1202 60-61 0.2069001525073162 1202 62-63 -0.20175234273903442 1202 64-65 0.076054643216942 1202 66-67 -0.11019973325722532 1202 68-69 0.033973648659888056 1202 70-71 -0.3695430116638363 1202 72-73 0.0603492063315727 1202 74-75 -0.06998915092525237 1202 76-77 -0.479131521959566 1202 78-79 -0.7627316374934523 1202 80-81 8.148799553140407E-4 1202 82-83 -0.06771141373066314 1202 84-85 0.16267611128162685 1202 86-87 -0.2942449906625555 1202 88-89 0.6091991617371093 1202 90-91 -0.0620854839604803 1203 1 0.13058006393771393 1203 2 0.12374059537241067 1203 3 0.34945787057697686 1203 4 0.022393000440892763 1203 5 0.10350594873502672 1203 6 0.00820197723666638 1203 7 0.16573324476149054 1203 8 0.08244015268531513 1203 9 0.15347120601651199 1203 10-11 -0.07473105247990475 1203 12-13 0.1194946228555196 1203 14-15 0.0333402302017376 1203 16-17 0.06057725222386523 1203 18-19 0.03875181480266576 1203 20-21 -0.002714335719964822 1203 22-23 0.03570627712319663 1203 24-25 -0.021070638047298473 1203 26-27 -0.002322509052874011 1203 28-29 -0.028961862602592703 1203 30-31 -0.013950300944493677 1203 32-33 0.06694177578339833 1203 34-35 -0.10919599414209102 1203 36-37 0.08756799678589289 1203 38-39 0.056039616698633665 1203 40-41 0.051713321440018944 1203 42-43 0.007444035103290503 1203 44-45 0.020641366100704772 1203 46-47 -0.1228792943081558 1203 48-49 0.1413981296796294 1203 50-51 -0.21632116537342228 1203 52-53 0.002981094344129076 1203 54-55 0.06641548870939573 1203 56-57 -0.13500949765406745 1203 58-59 0.16675829732446346 1203 60-61 0.3431008024484541 1203 62-63 0.10576479094741842 1203 64-65 -0.17905145666362898 1203 66-67 -0.07159548600846222 1203 68-69 0.06716329017981337 1203 70-71 0.09447525565483517 1203 72-73 0.07503663543950267 1203 74-75 -0.08096188189798426 1203 76-77 0.2173049683793593 1203 78-79 -0.18846401084906006 1203 80-81 0.21380785039660566 1203 82-83 -0.09485766802496443 1203 84-85 0.3848983335038483 1203 86-87 0.3439359188826714 1203 88-89 0.6091991617371093 1203 90-91 0.5532991314241329 1204 1 0.07648241908852071 1204 2 0.12472669595049268 1204 3 0.00616951763653617 1204 4 -0.0030617653288800284 1204 5 -0.1013606247534895 1204 6 0.05007364627456923 1204 7 0.02085167084468509 1204 8 0.13401822698966726 1204 9 -0.14096338438613998 1204 10-11 -0.12242322196384947 1204 12-13 -0.08674873088926205 1204 14-15 -0.18918631825821564 1204 16-17 -0.15333971894314402 1204 18-19 -0.1327314935647692 1204 20-21 -0.1271531254068563 1204 22-23 -0.07554550652800884 1204 24-25 -0.16744807640034765 1204 26-27 -0.025696435467210677 1204 28-29 -0.32198624561075206 1204 30-31 -0.10479272750401947 1204 32-33 -0.05182738654418273 1204 34-35 -0.15457022321958647 1204 36-37 -0.07315713423734849 1204 38-39 -0.1943581607307152 1204 40-41 -0.2124577298611996 1204 42-43 -0.11014979866510544 1204 44-45 -0.2792519520190879 1204 46-47 -0.25696223446431077 1204 48-49 -0.053860228614091454 1204 50-51 -0.04219573661285381 1204 52-53 -0.12268672334813857 1204 54-55 -0.23795451564258485 1204 56-57 0.07967314175893137 1204 58-59 0.025094118003799792 1204 60-61 -0.19886433676521165 1204 62-63 0.1307520436379761 1204 64-65 -0.013224370636251592 1204 66-67 0.05378880979544931 1204 68-69 0.037489882355146165 1204 70-71 -0.2686241290907603 1204 72-73 -0.24470289571657844 1204 74-75 -0.29344905597908877 1204 76-77 0.33125808843004023 1204 78-79 0.05582779040476282 1204 80-81 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62-63 0.02193890899069828 2401 64-65 0.2740271283615243 2401 66-67 0.3084582630933568 2401 68-69 0.2666545400805731 2401 70-71 0.3475208562580292 2401 72-73 -0.007769618271169065 2401 74-75 0.3227824671543118 2401 76-77 0.19824993676885327 2401 78-79 0.16492339814907098 2401 80-81 0.46401536510701646 2401 82-83 0.3481834276423612 2401 84-85 0.20141880134010393 2401 86-87 0.495451070397813 2401 88-89 -1.6765151239771754 2401 90-91 -0.51336753524253 2402 1 0.3530630023626813 2402 2 0.31394073339863837 2402 3 0.34002607853648925 2402 4 0.29632904828998363 2402 5 0.27395300034149983 2402 6 0.11787148110906998 2402 7 0.10213382753888567 2402 8 0.1610690921838227 2402 9 0.22480009183815497 2402 10-11 0.15129455905295686 2402 12-13 0.1405512902889967 2402 14-15 0.17090636776787704 2402 16-17 0.11992086084283926 2402 18-19 0.09769668099391282 2402 20-21 0.13663080652373338 2402 22-23 0.004451005288210297 2402 24-25 0.11010795255158712 2402 26-27 0.07409801084590129 2402 28-29 0.10690961964569823 2402 30-31 0.05987072505044466 2402 32-33 0.04729833270335604 2402 34-35 0.09899869315242427 2402 36-37 -0.017101275611196343 2402 38-39 0.007847563696913085 2402 40-41 0.1573175400584219 2402 42-43 0.11389762227085498 2402 44-45 0.07364137553069838 2402 46-47 0.14695403264249052 2402 48-49 0.15087705516913275 2402 50-51 0.15394099934546546 2402 52-53 0.005815600573839674 2402 54-55 0.08650611736030811 2402 56-57 -0.07443478153268757 2402 58-59 0.016581012073757506 2402 60-61 0.10763775192106095 2402 62-63 0.19200564909225193 2402 64-65 0.28146395657982737 2402 66-67 0.2180244839291845 2402 68-69 0.2533094341818938 2402 70-71 0.39055208245756745 2402 72-73 0.2794848838113779 2402 74-75 0.3832415886589047 2402 76-77 0.48208941050030063 2402 78-79 0.18735236496731034 2402 80-81 0.4344109671745855 2402 82-83 0.5209197460503248 2402 84-85 0.6071205557260697 2402 86-87 0.677269252216 2402 88-89 0.13861092644299333 2402 90-91 0.5532991314241329 2403 1 -0.26132544482687337 2403 2 -0.15281792354334556 2403 3 -0.05844513069758506 2403 4 -0.017550532059139812 2403 5 0.01618456760756004 2403 6 0.04803041986252765 2403 7 0.026317778886252086 2403 8 0.10982174785546306 2403 9 -0.058093367361237824 2403 10-11 0.0597306513644682 2403 12-13 0.005226294486966765 2403 14-15 0.11542747077508153 2403 16-17 0.002765404578369157 2403 18-19 0.11074542853901193 2403 20-21 0.04918017828855881 2403 22-23 0.06570922783166822 2403 24-25 0.054185896057873606 2403 26-27 0.08535148785929181 2403 28-29 0.07439251748538567 2403 30-31 0.0069127996501805455 2403 32-33 -0.009131374068836351 2403 34-35 0.06803860846557797 2403 36-37 0.029511398858161897 2403 38-39 0.07535305183254337 2403 40-41 0.010771935895249385 2403 42-43 0.039941754224436465 2403 44-45 0.020293525954222957 2403 46-47 0.09742134220656595 2403 48-49 0.053584937576154346 2403 50-51 -0.046490567957903295 2403 52-53 0.022789328823982657 2403 54-55 -0.06545405204448684 2403 56-57 -0.16800973092394145 2403 58-59 -0.0980114455224026 2403 60-61 -0.025062991402769796 2403 62-63 0.10106568466919441 2403 64-65 0.2427589322312329 2403 66-67 0.10595370479155974 2403 68-69 -0.3575823537866043 2403 70-71 -0.05195341619172211 2403 72-73 0.09444950992032375 2403 74-75 0.04669040277833858 2403 76-77 0.12329929779995297 2403 78-79 0.17390833469993083 2403 80-81 0.3893579158667677 2403 82-83 0.3317133968439734 2403 84-85 -0.544318743397838 2403 86-87 -0.6346965597498126 2403 88-89 0.6091991617371093 2403 90-91 0.5532991314241329 2404 1 -0.2651115749516819 2404 2 -0.2575809348972271 2404 3 -0.39374170684420307 2404 4 -0.5399476976641822 2404 5 -0.259047253088859 2404 6 -0.2843804286697207 2404 7 -0.11860401685487432 2404 8 -0.06139207866444707 2404 9 0.11422480491877707 2404 10-11 0.011994750935940601 2404 12-13 -0.032766113135934916 2404 14-15 0.05649342109686728 2404 16-17 0.09750675563302735 2404 18-19 0.03200391100006783 2404 20-21 0.05995726103297727 2404 22-23 -0.003405335901462081 2404 24-25 -0.030158511370800056 2404 26-27 0.09236671943719443 2404 28-29 0.04751640433850213 2404 30-31 -0.01212884049750329 2404 32-33 0.07810916383176192 2404 34-35 0.05477682040213239 2404 36-37 0.006982347095686237 2404 38-39 -0.0215815584828718 2404 40-41 0.04270420248160178 2404 42-43 0.08831742727781489 2404 44-45 0.05957462239332045 2404 46-47 0.16919415892463974 2404 48-49 0.0049828317043321135 2404 50-51 -0.08473906803470754 2404 52-53 -0.02378362177744009 2404 54-55 -0.011462006520389423 2404 56-57 -0.33089675323118684 2404 58-59 0.030168417652447488 2404 60-61 -0.39701299941893353 2404 62-63 0.03939310519951533 2404 64-65 0.08700503700364948 2404 66-67 0.06674178262067443 2404 68-69 0.1439760311302294 2404 70-71 0.0331645417449451 2404 72-73 0.12017171992852127 2404 74-75 -0.029744538194037773 2404 76-77 -0.07797180448405783 2404 78-79 0.3925623844934165 2404 80-81 0.36432431737779325 2404 82-83 0.2598671144713762 2404 84-85 0.04898102084234779 2404 86-87 -0.6892697005298487 2404 88-89 -0.19080083826288785 2404 90-91 -5.119428141303139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 4.0 31 237.0 32 1550.0 33 2910.0 34 3847.0 35 4707.0 36 6903.0 37 10703.0 38 27430.0 39 149228.0 40 176435.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.081781481963795 11.74449798150801 32.929808568824065 29.24391196770413 2 26.441769243992518 17.79480020211597 29.68526168287588 26.078168871015635 3 26.56373713380839 19.71939200733425 23.08726090761345 30.629609951243907 4 26.244550128400796 20.828797199664542 22.106293983133398 30.820358688801264 5 27.297280403381656 20.957458445542958 21.172588382983378 30.572672768092012 6 27.71660146788417 23.141834698948312 23.547872922277147 25.593690910890366 7 26.24168520187314 22.806638295212448 23.011350318006844 27.940326184907565 8 25.40174083353735 23.849211103413428 23.13323991936534 27.61580814368388 9 24.96549065773504 24.049755960349415 23.70023492397527 27.284518457940276 10-11 24.78734431728801 22.47599972913422 23.765737562312154 28.97091839126562 12-13 25.26435458414289 22.487980330977148 23.50281544143309 28.744849643446873 14-15 25.841246607666545 22.23716903587409 23.06239809977237 28.859186256686996 16-17 25.787594347239512 21.983623038176447 22.872661829281633 29.35612078530241 18-19 25.90297275194424 22.37976424259156 22.841408085343556 28.87585492012064 20-21 26.000640701750733 22.34056683873589 22.71535131812665 28.943441141386728 22-23 25.76910254874282 21.88790844736609 23.039478687551114 29.303510316339974 24-25 25.85986863009631 22.290560848434968 23.148476119535154 28.701094401933563 26-27 26.04713064585862 22.49709600629242 23.134021262963792 28.321752084885173 28-29 26.062887741760733 22.130515634685406 23.19249180891461 28.61410481463925 30-31 26.16180794845149 22.30738978353896 23.358050157703236 28.172752110306316 32-33 26.15372764608343 22.250736853201005 23.60957388870645 27.98596161200912 34-35 26.07469626518674 22.08232445520581 23.68624425921561 28.15673502039184 36-37 26.034472522905823 22.470008174399364 23.813227913518812 27.682291389176 38-39 26.203707181337048 22.359519186647482 23.838015346705227 27.598758285310243 40-41 26.065780760231338 22.11469692287316 24.020661339455213 27.798860977440288 42-43 25.98810503881635 22.425194851159105 24.09633687514907 27.49036323487547 44-45 26.138840062890694 22.487638943335146 24.13472603346021 27.23879496031395 46-47 26.384441273662734 22.191263205350463 23.989235867428274 27.43505965355853 48-49 26.151216569475167 22.551521807504113 24.211769194676897 27.085492428343827 50-51 26.324613101304163 22.370796935239863 24.182496189144732 27.122093774311246 52-53 26.186633055310832 22.315325106268048 24.188600863005856 27.309440975415267 54-55 26.262969278344556 22.596529002051962 24.33311175977573 26.80738995982775 56-57 26.540284360189574 22.608058906087912 23.992026524011962 26.859630209710556 58-59 26.236358454310594 22.319729329668007 24.1805878621273 27.263324353894102 60-61 26.293105694615992 22.75032181068672 24.071439366697415 26.88513312799988 62-63 26.67259680958311 22.535136096779933 23.83265136689794 26.959615726739017 64-65 26.631319442099223 22.4247745770153 23.771571375502347 27.172334605383135 66-67 26.72480440362433 22.707734174475057 23.725917679438414 26.841543742462193 68-69 27.07406948110617 22.631849345403655 23.42905734582883 26.865023827661343 70-71 27.004024948535964 22.55814668018558 23.36313638737825 27.074691983900205 72-73 26.820496385713778 23.023418675592588 23.303540694845044 26.85254424384859 74-75 27.15929571606821 22.452516290031888 23.015388881186745 27.37279911271316 76-77 27.28520091473375 22.533485788957858 22.835674616138515 27.34563868016988 78-79 27.24661469019286 22.924360552591985 22.818844402759055 27.010180354456104 80-81 27.870463588858524 22.906179586749126 21.909008102018312 27.31434872237404 82-83 28.158571644726887 22.696323195642307 21.667423210773187 27.47768194885762 84-85 27.731824118415325 23.156686587248192 21.518185775913246 27.59330351842324 86-87 28.563526743735828 21.914928923059904 20.609546988218376 28.911997344985895 88-89 27.875507442489848 21.768155164636894 20.31574199368516 30.040595399188092 90-91 24.892148403796376 28.106125970664365 19.305435720448664 27.696289905090595 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 2.0 8 3.0 9 3.5 10 5.0 11 6.0 12 10.5 13 19.0 14 25.0 15 34.0 16 42.5 17 56.5 18 93.5 19 142.0 20 194.5 21 264.5 22 340.5 23 430.0 24 569.0 25 735.5 26 931.0 27 1193.5 28 1476.5 29 1810.0 30 2224.0 31 2735.5 32 3335.5 33 3846.5 34 4450.5 35 5289.0 36 6325.0 37 7351.0 38 8445.5 39 9882.0 40 11377.5 41 12789.0 42 14176.5 43 15657.0 44 17180.5 45 18800.5 46 20665.5 47 22455.0 48 23984.0 49 25374.5 50 26575.0 51 27582.5 52 28149.0 53 28564.0 54 29168.5 55 29525.5 56 29627.5 57 29404.5 58 28758.0 59 28035.0 60 27405.0 61 26595.5 62 25711.0 63 24687.0 64 23246.0 65 21718.0 66 19767.5 67 17782.0 68 15995.5 69 14072.0 70 12210.5 71 10354.5 72 8676.0 73 7133.5 74 5618.5 75 4456.0 76 3459.0 77 2522.5 78 1939.0 79 1454.5 80 1011.0 81 703.0 82 499.0 83 335.5 84 220.0 85 153.0 86 102.5 87 62.5 88 39.5 89 26.5 90 20.0 91 17.5 92 11.5 93 7.5 94 7.5 95 7.5 96 5.0 97 2.5 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0010417914646025305 2 0.004167165858410122 3 5.208957323012653E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 9088.0 32-33 19655.0 34-35 21231.0 36-37 22043.0 38-39 22966.0 40-41 23294.0 42-43 22773.0 44-45 22376.0 46-47 21435.0 48-49 20146.0 50-51 18899.0 52-53 17596.0 54-55 15829.0 56-57 14511.0 58-59 13124.0 60-61 11932.0 62-63 10607.0 64-65 9597.0 66-67 8442.0 68-69 7810.0 70-71 6893.0 72-73 6199.0 74-75 5635.0 76-77 5064.0 78-79 4798.0 80-81 4498.0 82-83 3958.0 84-85 3626.0 86-87 3523.0 88-89 3308.0 90-91 3098.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.87119259567574 #Duplication Level Percentage of deduplicated Percentage of total 1 99.87365336677094 99.74500870626538 2 0.12371973467532947 0.24712074899291464 3 0.0026268985537421592 0.007870544741702557 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002604478661506326 0.0 0.0 0.0 0.0 2 0.002604478661506326 0.0 0.0 0.0 0.0 3 0.002604478661506326 0.0 0.0 0.0 0.0 4 0.0031253743938075913 0.0 0.0 0.0 0.0 5 0.0031253743938075913 0.0 0.0 0.0 0.0 6 0.0031253743938075913 0.0 0.0 0.0 0.0 7 0.0033858222599582244 0.0 0.0 0.0 0.0 8 0.009115675315272142 0.0 0.0 0.0 0.0 9 0.009376123181422774 0.0 0.0 0.0 0.0 10-11 0.009376123181422774 0.0 0.0 0.0 0.0 12-13 0.009376123181422774 0.0 0.0 0.0 0.0 14-15 0.00950634711449809 0.0 0.0 0.0 0.0 16-17 0.009636571047573407 0.0 0.0 0.0 0.0 18-19 0.009636571047573407 2.6044786615063263E-4 0.0 0.0 0.0 20-21 0.00989701891372404 2.6044786615063263E-4 0.0 0.0 0.0 22-23 0.010157466779874672 2.6044786615063263E-4 0.0 0.0 0.0 24-25 0.01028769071294999 2.6044786615063263E-4 0.0 0.0 0.0 26-27 0.010678362512175937 2.6044786615063263E-4 0.0 0.0 0.0 28-29 0.01093881037832657 2.6044786615063263E-4 0.0 0.0 0.0 30-31 0.011720153976778468 2.6044786615063263E-4 0.0 0.0 0.0 32-33 0.01263172150830568 2.6044786615063263E-4 0.0 0.0 1.3022393307531632E-4 34-35 0.013022393307531632 2.6044786615063263E-4 0.0 0.0 2.6044786615063263E-4 36-37 0.01341306510675758 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 38-39 0.013673512972908215 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 40-41 0.01380373690598353 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 42-43 0.014324632638284795 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 44-45 0.014324632638284795 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 46-47 0.014324632638284795 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 48-49 0.014324632638284795 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 50-51 0.014324632638284795 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 52-53 0.014324632638284795 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 54-55 0.014585080504435428 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 56-57 0.01484552837058606 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 58-59 0.01484552837058606 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 60-61 0.014975752303661376 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 62-63 0.015105976236736693 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 64-65 0.015366424102887325 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 66-67 0.015366424102887325 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 68-69 0.015366424102887325 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 70-71 0.015366424102887325 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 72-73 0.015366424102887325 2.6044786615063263E-4 2.6044786615063263E-4 0.0 2.6044786615063263E-4 74-75 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