##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-8C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 494034 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.25787293991912 40.0 40.0 40.0 40.0 40.0 2 39.1272382062773 40.0 40.0 40.0 40.0 40.0 3 39.11358732394936 40.0 40.0 40.0 40.0 40.0 4 39.08309144714736 40.0 40.0 40.0 40.0 40.0 5 39.205309756008695 40.0 40.0 40.0 40.0 40.0 6 39.25554516490768 40.0 40.0 40.0 40.0 40.0 7 39.296890497415156 40.0 40.0 40.0 40.0 40.0 8 39.314694940024374 40.0 40.0 40.0 40.0 40.0 9 39.351162065768754 40.0 40.0 40.0 40.0 40.0 10-11 39.33330904350713 40.0 40.0 40.0 40.0 40.0 12-13 39.337231850439444 40.0 40.0 40.0 40.0 40.0 14-15 39.34910552714995 40.0 40.0 40.0 40.0 40.0 16-17 39.489577640405315 40.0 40.0 40.0 40.0 40.0 18-19 39.49590918843642 40.0 40.0 40.0 40.0 40.0 20-21 39.5052040952647 40.0 40.0 40.0 40.0 40.0 22-23 39.50532959270009 40.0 40.0 40.0 40.0 40.0 24-25 39.49852844136234 40.0 40.0 40.0 40.0 40.0 26-27 39.49468660051737 40.0 40.0 40.0 40.0 40.0 28-29 39.49473518016978 40.0 40.0 40.0 40.0 40.0 30-31 39.48223542166616 40.0 40.0 40.0 40.0 40.0 32-33 39.482942552591965 40.0 40.0 40.0 40.0 40.0 34-35 39.4774719453023 40.0 40.0 40.0 40.0 40.0 36-37 39.46817708081767 40.0 40.0 40.0 40.0 40.0 38-39 39.45984532161497 40.0 40.0 40.0 40.0 40.0 40-41 39.44885359770379 40.0 40.0 40.0 40.0 40.0 42-43 39.438912398307316 40.0 40.0 40.0 40.0 40.0 44-45 39.42873598186016 40.0 40.0 40.0 40.0 40.0 46-47 39.42146258370476 40.0 40.0 40.0 40.0 40.0 48-49 39.42047897216754 40.0 40.0 40.0 40.0 40.0 50-51 39.41934848768011 40.0 40.0 40.0 40.0 40.0 52-53 39.3950059830987 40.0 40.0 40.0 40.0 40.0 54-55 39.38567070826551 40.0 40.0 40.0 40.0 40.0 56-57 39.386170084615785 40.0 40.0 40.0 40.0 40.0 58-59 39.35936719708779 40.0 40.0 40.0 40.0 40.0 60-61 39.339258824434964 40.0 40.0 40.0 40.0 40.0 62-63 39.355573425967144 40.0 40.0 40.0 40.0 40.0 64-65 39.36857028217756 40.0 40.0 40.0 40.0 40.0 66-67 39.35782908174407 40.0 40.0 40.0 40.0 40.0 68-69 39.35237835453755 40.0 40.0 40.0 40.0 40.0 70-71 39.352670899294324 40.0 40.0 40.0 40.0 40.0 72-73 39.349042413300566 40.0 40.0 40.0 40.0 40.0 74-75 39.348000685307525 40.0 40.0 40.0 40.0 40.0 76-77 39.32746145237667 40.0 40.0 40.0 40.0 40.0 78-79 39.324690746216405 40.0 40.0 40.0 40.0 40.0 80-81 39.31453394004199 40.0 40.0 40.0 40.0 40.0 82-83 39.293202411534395 40.0 40.0 40.0 40.0 40.0 84-85 39.28631657677843 40.0 40.0 40.0 40.0 40.0 86-87 39.307209528123316 40.0 40.0 40.0 40.0 40.0 88-89 39.32585474861759 40.0 40.0 40.0 40.0 40.0 90-91 39.44415352627641 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1746570754446921 1101 2 0.01987854627114416 1101 3 0.3414364608761744 1101 4 0.17317303580943388 1101 5 0.1297160246853295 1101 6 0.01781553683525061 1101 7 0.09939559995984837 1101 8 0.12527482491142194 1101 9 0.19312717812371005 1101 10-11 0.08694150669951028 1101 12-13 0.05689021623712165 1101 14-15 -0.08880306281904637 1101 16-17 -0.01669059931179362 1101 18-19 0.09669309450430319 1101 20-21 0.0310239869439215 1101 22-23 0.1566394578197503 1101 24-25 0.14967848917848414 1101 26-27 0.0823327482017362 1101 28-29 0.031128143812566123 1101 30-31 -0.013695762591964922 1101 32-33 0.14393583161221102 1101 34-35 0.11489141576181794 1101 36-37 -0.015066407576547647 1101 38-39 0.1339247107259922 1101 40-41 0.07664023933746478 1101 42-43 0.16392812403109502 1101 44-45 -0.06695676667145989 1101 46-47 0.06859648208049407 1101 48-49 0.032948937424123415 1101 50-51 -0.03494616012812202 1101 52-53 -0.05381881957393375 1101 54-55 0.019409421873660904 1101 56-57 0.019863255369507726 1101 58-59 0.023162309578097506 1101 60-61 -0.16643014958935964 1101 62-63 -0.09838717935698327 1101 64-65 -0.10760243367421651 1101 66-67 -0.3778531937312053 1101 68-69 0.1067519659353664 1101 70-71 -0.11779558689048031 1101 72-73 -0.18690179216969938 1101 74-75 -0.162276787073246 1101 76-77 -0.12993533298331528 1101 78-79 -0.3650486361522667 1101 80-81 -0.03624850052406714 1101 82-83 0.46678142729302863 1101 84-85 0.368011834012826 1101 86-87 -0.5315609655550446 1101 88-89 -0.22064985691356753 1101 90-91 -1.2188727447068501 1102 1 0.45503394397350405 1102 2 0.4028542096196759 1102 3 0.41610969721801894 1102 4 0.2966420906418037 1102 5 0.0508239915262223 1102 6 0.1380219489270047 1102 7 0.147882466158201 1102 8 0.1542018149570339 1102 9 -0.029310482095354473 1102 10-11 0.11749049627902508 1102 12-13 0.10863972517610421 1102 14-15 -0.009406318803328872 1102 16-17 0.10696356055770195 1102 18-19 0.014728850405347771 1102 20-21 0.03716707713268619 1102 22-23 0.0061450675677292566 1102 24-25 0.18203857840178017 1102 26-27 0.008521115881606534 1102 28-29 0.11072330977454925 1102 30-31 0.05770417748193779 1102 32-33 0.043322027603693414 1102 34-35 0.08335805568138 1102 36-37 0.1004025792518064 1102 38-39 0.07195267161822727 1102 40-41 0.07985127942550463 1102 42-43 0.05029490978429152 1102 44-45 0.025842811560302437 1102 46-47 0.12579499643835845 1102 48-49 0.020100361244132614 1102 50-51 0.13216539889747736 1102 52-53 -0.04488695498697126 1102 54-55 0.02574883889388957 1102 56-57 0.04538468343676527 1102 58-59 0.14500898101892545 1102 60-61 0.20343527512247306 1102 62-63 0.06229979276550779 1102 64-65 0.10687960032755939 1102 66-67 -0.018764183100280718 1102 68-69 0.1242738552001228 1102 70-71 -0.01633119558943008 1102 72-73 0.31681043451300894 1102 74-75 0.05581983310069205 1102 76-77 0.11323365943002983 1102 78-79 0.11835545975183237 1102 80-81 -0.07553200077285993 1102 82-83 0.06280388309809126 1102 84-85 -0.001604211659561372 1102 86-87 -0.2583282162505114 1102 88-89 0.339387145861636 1102 90-91 0.714460588626487 1103 1 0.31234692409285714 1103 2 0.2640285856705873 1103 3 0.19790126360224747 1103 4 0.038281997008226654 1103 5 0.019629504038178425 1103 6 -0.22373071336569694 1103 7 -0.12092502452789233 1103 8 -0.011418837181039976 1103 9 0.10737080108027897 1103 10-11 0.031149788752074414 1103 12-13 -0.03900459911258736 1103 14-15 0.04275417705898832 1103 16-17 -0.039078442673321945 1103 18-19 0.016623941236517226 1103 20-21 -0.00637781093562495 1103 22-23 0.014690779875273563 1103 24-25 0.03360957836880374 1103 26-27 -0.03638211843312433 1103 28-29 0.04239717479713079 1103 30-31 -0.03274190078781203 1103 32-33 0.027906725358384676 1103 34-35 0.1250132862526243 1103 36-37 -0.07178834076671592 1103 38-39 0.013797459275608048 1103 40-41 0.039400858525539206 1103 42-43 0.15856303392703097 1103 44-45 0.0367057514284852 1103 46-47 -0.011571021346846067 1103 48-49 0.0495765998851212 1103 50-51 0.04307848946505288 1103 52-53 0.08096483976635227 1103 54-55 0.13691461959659534 1103 56-57 -0.06379421243203609 1103 58-59 0.08860381963353348 1103 60-61 0.05520701107111847 1103 62-63 -0.004574700408234378 1103 64-65 -0.14188714700753735 1103 66-67 -0.2681877243481239 1103 68-69 -0.21853817389332875 1103 70-71 -0.24169316353945192 1103 72-73 -0.4055223052110364 1103 74-75 -0.18988033120783143 1103 76-77 -0.561381874512989 1103 78-79 -0.564669848273482 1103 80-81 -0.9230276352260276 1103 82-83 -0.46910945276990645 1103 84-85 -0.6825716154691293 1103 86-87 -0.6342946766159514 1103 88-89 -0.3104007650609475 1103 90-91 0.714460588626487 1104 1 0.3287411591430356 1104 2 0.14713188919544962 1104 3 0.2808951704624363 1104 4 0.23381708662444822 1104 5 0.18348026663469597 1104 6 -0.006692818869879602 1104 7 0.12345694791941497 1104 8 0.03774351510165985 1104 9 0.008394804743794282 1104 10-11 0.10852375997821184 1104 12-13 -0.09216374581859554 1104 14-15 -0.05375148994265544 1104 16-17 0.0345226156758045 1104 18-19 0.025726761380767016 1104 20-21 0.08841567105844916 1104 22-23 0.01777932757094902 1104 24-25 0.006206985922268871 1104 26-27 -0.005504273801832937 1104 28-29 0.06405771440253716 1104 30-31 -0.01090581769723542 1104 32-33 -0.019373282687510596 1104 34-35 0.007549072889055708 1104 36-37 0.08207800909828222 1104 38-39 -0.033827878745128714 1104 40-41 -0.10210097265355245 1104 42-43 0.01221156403698842 1104 44-45 0.10668742710513612 1104 46-47 0.018334738150464602 1104 48-49 0.1453236873186654 1104 50-51 -0.051150152944067884 1104 52-53 -0.0682168923041715 1104 54-55 0.12272292483902447 1104 56-57 -0.07703964459541623 1104 58-59 -0.041505505717161384 1104 60-61 0.04834041161922897 1104 62-63 0.09798812827600756 1104 64-65 0.033220203856700437 1104 66-67 0.0748429486999953 1104 68-69 0.0728944460919152 1104 70-71 0.17197489130121824 1104 72-73 -0.33992756433025306 1104 74-75 0.26632794616815403 1104 76-77 -0.21873565967604947 1104 78-79 -0.0790848728644562 1104 80-81 -0.3314395161906205 1104 82-83 -0.01575012977188095 1104 84-85 -0.11117399405953421 1104 86-87 0.4212980398463344 1104 88-89 -0.4204472226683862 1104 90-91 -0.9485430743771772 1201 1 0.32771513234548877 1201 2 0.2889430977982883 1201 3 0.042916147284344675 1201 4 0.1686865826751074 1201 5 0.16478240809567524 1201 6 0.18547756077236954 1201 7 0.15482475756184755 1201 8 -0.07771163013909188 1201 9 0.16868929416597211 1201 10-11 0.008203743206621539 1201 12-13 0.15943479653275716 1201 14-15 0.08636406139260089 1201 16-17 0.05590639532669428 1201 18-19 0.011114511952669659 1201 20-21 0.09696794971351608 1201 22-23 0.0925337655684686 1201 24-25 -0.0959727044198857 1201 26-27 0.03486346801430784 1201 28-29 0.14647035722313007 1201 30-31 0.05935728665043172 1201 32-33 0.1660423709762 1201 34-35 0.06810611897292773 1201 36-37 0.0662053962474829 1201 38-39 0.007914384339159142 1201 40-41 -0.00711760980727405 1201 42-43 -0.058677735508545936 1201 44-45 0.03349584387378712 1201 46-47 -0.012377278435089067 1201 48-49 -0.0848352637903389 1201 50-51 0.08469656258122171 1201 52-53 -0.05075641993204982 1201 54-55 -0.048644725526891364 1201 56-57 0.023284821540734413 1201 58-59 -0.05660491044760363 1201 60-61 0.17113747218125752 1201 62-63 0.06517927000376744 1201 64-65 0.16895523680329916 1201 66-67 0.16398279424473827 1201 68-69 -0.1191258432370006 1201 70-71 0.08290062839564172 1201 72-73 -0.1336070501520723 1201 74-75 0.09180929445226127 1201 76-77 0.255378924032577 1201 78-79 0.03226142822435918 1201 80-81 -0.2815416823620822 1201 82-83 -0.25965176361406606 1201 84-85 0.07442861019703884 1201 86-87 0.39784280655503323 1201 88-89 -0.6643401286679946 1201 90-91 0.18112725529315554 1202 1 -0.002279320549497754 1202 2 0.02434558744479176 1202 3 0.17907804342065958 1202 4 -0.26444676239749043 1202 5 0.09402226239251377 1202 6 -0.03012753342925123 1202 7 0.05217867428074641 1202 8 -0.05011913982549032 1202 9 -0.11275747255692181 1202 10-11 0.022832955595831095 1202 12-13 0.10963585655267138 1202 14-15 0.04389234832277111 1202 16-17 0.04648773553851271 1202 18-19 0.04088137127556024 1202 20-21 -0.05450708140256211 1202 22-23 -0.0019304880574324557 1202 24-25 -0.03939704112615772 1202 26-27 -0.055593407901817216 1202 28-29 -0.033195746719883346 1202 30-31 0.008714561681664179 1202 32-33 -0.0467429672803803 1202 34-35 -0.08803612950928397 1202 36-37 0.08642323897159088 1202 38-39 0.09053251222888292 1202 40-41 0.006990194555221763 1202 42-43 0.01465593455827019 1202 44-45 0.027538332424782652 1202 46-47 -0.0019456615138437883 1202 48-49 -0.13373113180971075 1202 50-51 -0.013035133499400331 1202 52-53 -0.14399217755146765 1202 54-55 -0.004784165351154002 1202 56-57 0.038201439285444394 1202 58-59 -0.04939961696435802 1202 60-61 -0.09277193363598002 1202 62-63 0.0526738452397737 1202 64-65 0.03978766437077752 1202 66-67 0.18253081654319914 1202 68-69 0.05660039275702644 1202 70-71 -0.187088465180409 1202 72-73 -0.10403537137658958 1202 74-75 0.11444068311675437 1202 76-77 0.09844634572390731 1202 78-79 0.2116004127683624 1202 80-81 -0.014839243463121932 1202 82-83 -0.11818876475680895 1202 84-85 -0.35008326643521315 1202 86-87 -0.764285867771477 1202 88-89 -0.18655618877734526 1202 90-91 -0.909781835615938 1203 1 0.08468830649611903 1203 2 -0.05843502769266706 1203 3 -0.09750822895711764 1203 4 0.13042378994671822 1203 5 -0.06113589040477052 1203 6 -0.18993818691582476 1203 7 -0.1368288678310705 1203 8 -0.16565345530037234 1203 9 -0.1325586491693329 1203 10-11 -0.06185377642228218 1203 12-13 0.010553585588070291 1203 14-15 -0.07920795018683435 1203 16-17 0.02951410488118711 1203 18-19 0.03477391975502542 1203 20-21 0.01273695049415835 1203 22-23 -0.030500757169626525 1203 24-25 0.024898895156788114 1203 26-27 -0.03185467362548877 1203 28-29 -0.04993305230372158 1203 30-31 0.03459693147311782 1203 32-33 0.007840454672660258 1203 34-35 0.014096778458331016 1203 36-37 -0.051211401600284034 1203 38-39 0.06392981710455814 1203 40-41 0.04406251818263485 1203 42-43 0.07364859121404521 1203 44-45 0.04196596101565575 1203 46-47 0.005247892133390053 1203 48-49 0.0771986773481288 1203 50-51 -0.013993036272601955 1203 52-53 -0.01643968785680272 1203 54-55 -0.10012161903485861 1203 56-57 -0.15781963192804227 1203 58-59 -0.21025004723347962 1203 60-61 -0.19034423011139268 1203 62-63 -0.22373810835793506 1203 64-65 -0.09657711552871717 1203 66-67 -0.02529167539946542 1203 68-69 -0.34037579100217386 1203 70-71 -0.02249354503670986 1203 72-73 -0.0323950317275461 1203 74-75 -0.2126395493090243 1203 76-77 -0.09087896598182255 1203 78-79 0.1704841120874292 1203 80-81 -0.23339893791415278 1203 82-83 -0.40519831710050624 1203 84-85 0.3986546602910792 1203 86-87 0.4775730719553195 1203 88-89 -0.18655618877734526 1203 90-91 0.07446058862648641 1204 1 0.3199999821785795 1204 2 -0.042293061779361096 1204 3 -0.16602748667305178 1204 4 -0.209701692873125 1204 5 -0.062272982221387 1204 6 -0.12765161371968503 1204 7 -0.21704595761038803 1204 8 -0.014019892614037133 1204 9 -0.15851513882476098 1204 10-11 -0.1108944144255446 1204 12-13 -0.09025671077832698 1204 14-15 -0.16017244094145155 1204 16-17 -0.14954824446895287 1204 18-19 -0.0713653100310978 1204 20-21 0.006119313015616967 1204 22-23 -0.09093126486927616 1204 24-25 -0.0458862548978729 1204 26-27 -0.15035977691725577 1204 28-29 -0.10356706745783129 1204 30-31 -0.04678686188887582 1204 32-33 -0.14856332176574938 1204 34-35 -0.17146698788549486 1204 36-37 -0.19284910355327867 1204 38-39 0.006839848630022516 1204 40-41 -0.09141666001752213 1204 42-43 -0.1325540517345587 1204 44-45 -0.2841654249358996 1204 46-47 -0.12083582900707768 1204 48-49 -0.09420420445892574 1204 50-51 -0.1307921675019159 1204 52-53 0.008123544331276378 1204 54-55 0.01020505341085709 1204 56-57 -0.09015427682034982 1204 58-59 -0.05194219456867444 1204 60-61 0.00939598108317341 1204 62-63 -0.2199627170190368 1204 64-65 -0.14231621048553222 1204 66-67 -0.07527941966395701 1204 68-69 -0.24230722303229157 1204 70-71 -0.3087220684247711 1204 72-73 -0.10069080598086799 1204 74-75 -0.28470137935997286 1204 76-77 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50-51 0.0690358059433791 2401 52-53 0.12097611911693207 2401 54-55 0.13274487429869453 2401 56-57 0.21719180805272487 2401 58-59 0.08485989698632324 2401 60-61 0.06368458496452689 2401 62-63 0.04536011365450321 2401 64-65 0.14724243893157052 2401 66-67 -0.004949652583739805 2401 68-69 0.13932020005369594 2401 70-71 -0.04098507165890908 2401 72-73 0.22850509222912052 2401 74-75 -0.21115867415385736 2401 76-77 0.13551153005493433 2401 78-79 0.2199783603513339 2401 80-81 -0.13084586849361557 2401 82-83 0.2010777761054996 2401 84-85 0.5095536166142054 2401 86-87 0.09698319806160782 2401 88-89 0.3966987519244043 2401 90-91 0.24779392195981842 2402 1 0.2580750948600894 2402 2 0.22202700988732715 2402 3 0.1802465322799165 2402 4 0.1162038169716837 2402 5 0.0798336937677746 2402 6 0.26700990476507513 2402 7 0.04109926107289397 2402 8 0.1241382485468634 2402 9 0.06450684811542118 2402 10-11 0.09065877998595084 2402 12-13 0.09714340533669485 2402 14-15 0.10817732121005008 2402 16-17 0.10532421629540778 2402 18-19 -0.0053046056000027875 2402 20-21 -0.021925428722113338 2402 22-23 -0.03486363099365519 2402 24-25 -0.04266031755005173 2402 26-27 0.042565946928775134 2402 28-29 0.05778754530482644 2402 30-31 -0.021655140015553798 2402 32-33 -0.04875877034530873 2402 34-35 -7.946689092861448E-4 2402 36-37 0.0789857068515829 2402 38-39 -0.004069009519277245 2402 40-41 0.03953862528362606 2402 42-43 -0.028028948382967656 2402 44-45 0.0655076124629872 2402 46-47 0.07996879982531624 2402 48-49 0.0219241104980199 2402 50-51 0.1075494914105235 2402 52-53 0.046932994271230655 2402 54-55 0.05379922751988886 2402 56-57 0.12767238189183416 2402 58-59 0.02870957864421797 2402 60-61 0.07078175467749759 2402 62-63 0.1422460557084051 2402 64-65 0.14977569040108563 2402 66-67 0.02699850680038196 2402 68-69 0.05083971730775261 2402 70-71 0.29326928217439274 2402 72-73 0.1363833263896126 2402 74-75 0.14646396960147712 2402 76-77 0.12263383179502796 2402 78-79 0.24731482177570285 2402 80-81 0.37569145383317704 2402 82-83 0.04371990541739734 2402 84-85 -0.09448194844564028 2402 86-87 0.40908099719450775 2402 88-89 -0.022351984573148798 2402 90-91 0.4287463029122023 2403 1 -0.25349011397383236 2403 2 -0.14581931081400512 2403 3 0.022299731010427593 2403 4 0.08115965554670623 2403 5 -0.06996456601785184 2403 6 0.18066857836416972 2403 7 -0.019376673615767004 2403 8 -6.331535476249428E-4 2403 9 0.15900081641104435 2403 10-11 0.10514963196931859 2403 12-13 0.05943961491689009 2403 14-15 0.12492288661570683 2403 16-17 -0.008294068256709863 2403 18-19 0.05666900415569387 2403 20-21 0.11254190740496739 2403 22-23 0.03602939748779477 2403 24-25 -0.03170094442085514 2403 26-27 0.11038674257250136 2403 28-29 0.06782022557939626 2403 30-31 0.13318354982116887 2403 32-33 0.03853975916148755 2403 34-35 0.0181937848651188 2403 36-37 0.1029593124839252 2403 38-39 0.004677501419259045 2403 40-41 -0.13114128413979387 2403 42-43 0.011255280953328395 2403 44-45 0.04585228269241526 2403 46-47 -5.672271264529627E-4 2403 48-49 0.05059145804283105 2403 50-51 0.11952936180016849 2403 52-53 -0.03703333226017946 2403 54-55 0.06627885518810217 2403 56-57 0.03201939166675771 2403 58-59 -0.008430573458475976 2403 60-61 0.13155788112251798 2403 62-63 0.14143770557301139 2403 64-65 0.1887635390296083 2403 66-67 -0.009787964294162066 2403 68-69 0.21051803041483197 2403 70-71 -0.009105149135450574 2403 72-73 0.19072511770743716 2403 74-75 0.13297942978720556 2403 76-77 0.4125807704981739 2403 78-79 0.12137530724927359 2403 80-81 0.5010449204255849 2403 82-83 0.10319437668422182 2403 84-85 -0.17820877815267266 2403 86-87 0.028361416721033095 2403 88-89 0.3937349719485894 2403 90-91 -0.006127646667628994 2404 1 -0.3960977814439346 2404 2 -0.18389491427230098 2404 3 -0.3150962397021999 2404 4 -0.2238938813227378 2404 5 -0.09623022409942195 2404 6 -0.13604937657289184 2404 7 -0.07883367484365067 2404 8 -0.02745969813305038 2404 9 0.04087710809223921 2404 10-11 0.018974726334690217 2404 12-13 0.08697408922475347 2404 14-15 0.11786228560812617 2404 16-17 -0.017278863684722978 2404 18-19 -0.0011482883289346546 2404 20-21 -0.006382706417099371 2404 22-23 -0.08773248345266893 2404 24-25 -5.217184442400935E-4 2404 26-27 0.05309749188654678 2404 28-29 0.10381751715446796 2404 30-31 0.05269077818282142 2404 32-33 0.04206050670592276 2404 34-35 -0.020390155607621807 2404 36-37 0.013738108034360152 2404 38-39 -0.012680847454483057 2404 40-41 0.11069051720180312 2404 42-43 0.14261483648143525 2404 44-45 0.05073067176486745 2404 46-47 0.05526345651875886 2404 48-49 -0.05481192508647581 2404 50-51 -0.004082627528710248 2404 52-53 0.03466545750075056 2404 54-55 0.10076355553175631 2404 56-57 0.020059700155734106 2404 58-59 0.006260831700757308 2404 60-61 0.20517919596404965 2404 62-63 0.07256870971031759 2404 64-65 0.0443690681125517 2404 66-67 0.05565432571043516 2404 68-69 0.11367706809631528 2404 70-71 0.2948340252088997 2404 72-73 0.020923446220201924 2404 74-75 0.03681025915209801 2404 76-77 0.09500856693873772 2404 78-79 -0.09526244408345974 2404 80-81 0.09597117411289702 2404 82-83 -0.2788150052599221 2404 84-85 -0.35634763850193707 2404 86-87 0.04593386913332154 2404 88-89 -5.338027549584012E-4 2404 90-91 -0.9832138299781619 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 9.0 31 288.0 32 1996.0 33 3615.0 34 4632.0 35 5870.0 36 8701.0 37 13159.0 38 34608.0 39 211130.0 40 210026.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.648781040750727 12.537548479033578 26.71690673403127 32.09676374618443 2 26.155730321157094 18.760513770382293 29.387949270741608 25.695806637719006 3 26.7621941222074 19.607148568201463 21.366357035720576 32.26430027387056 4 27.482319030674002 21.03863296858111 20.300424667128173 31.178623333616716 5 28.023172494200804 21.03438224899501 20.798973350012346 30.14347190679184 6 27.773448332398846 23.743353176812075 23.345606467584147 25.13759202320493 7 26.11055109567358 23.818603577891402 22.799442953319 27.271402373116022 8 25.174583125857737 24.755988454235943 23.407093438913112 26.66233498099321 9 24.6683426646749 24.882295550508672 24.026079176736822 26.423282608079607 10-11 24.818028718671183 23.354566689742 23.849674313913617 27.977730277673196 12-13 25.19371136399519 23.10782253852974 23.725188954606363 27.973277142868707 14-15 25.562613099503274 22.88617787439731 23.527631701461843 28.02357732463758 16-17 25.78830606800342 22.699348627827234 23.110858766805524 28.401486537363823 18-19 25.74073849168276 23.001959379313973 23.204374597699754 28.052927531303514 20-21 25.70126752409753 23.131505119080874 23.05053903172656 28.116688325095033 22-23 25.705518243683635 22.621823599185483 23.169053142091435 28.50360501503945 24-25 25.68689604359214 23.01622965221017 23.267426938226922 28.02944736597076 26-27 25.867045587955484 23.12786164514993 23.199213009630917 27.805879757263668 28-29 25.998514272297047 22.78152920649186 23.207107203147963 28.012849318063125 30-31 25.934075053918626 22.882548987260932 23.278778782324608 27.904597176495834 32-33 25.969479362980906 22.8918246591579 23.370075933786648 27.768620044074545 34-35 25.985401303286533 22.577894750389312 23.431487368244937 28.005216578079217 36-37 25.93025640568364 23.003838728805626 23.456037415345108 27.60986745016563 38-39 25.990957361447382 23.06140295792894 23.497370703990807 27.450268976632874 40-41 26.072065200571686 22.66082957955671 23.4450104723971 27.8220947474745 42-43 25.906010833658204 23.036271210784413 23.622196688347664 27.435521267209715 44-45 26.028790836348463 23.02752156874432 23.693853195723232 27.249834399183985 46-47 26.115304781262793 22.787597589617256 23.61729787188198 27.47979975723797 48-49 26.025126358207356 22.98182429900825 23.696312099037307 27.29673724374708 50-51 26.111309970367248 23.111913085974212 23.70080889407557 27.075968049582965 52-53 26.355301201437232 22.889940770267238 23.485045194251068 27.269712834044462 54-55 26.221841642140042 23.100958948389184 23.590168179850554 27.087031229620216 56-57 26.393000245318504 23.087333387848556 23.57797039823371 26.941695968599234 58-59 26.526166179948703 22.723325819740392 23.399327107784895 27.35118089252601 60-61 26.36948658476944 23.12934887346927 23.381141686721318 27.12002285503997 62-63 26.73790549359593 23.064454513619918 23.153591476808472 27.04404851597568 64-65 26.747301225586867 22.921249835663264 23.15438888645427 27.177060052295605 66-67 26.71656541109761 23.254434993924665 23.11705143782908 26.91194815714864 68-69 26.638827579480473 23.07092103789704 23.186515863583658 27.10373551903883 70-71 26.866761009987144 22.835408751221532 22.880417813857115 27.417412424934213 72-73 26.778008079452547 23.15144607493945 22.868343528395275 27.202202317212727 74-75 26.80582416829591 23.140687496377023 22.792881572082777 27.260606763244294 76-77 27.348372894451224 22.95607704147019 22.307932867716044 27.387617196362545 78-79 27.209534705226417 22.884595890113392 22.468973107612992 27.4368962970472 80-81 27.358597829224134 23.10275763751017 21.977137759430455 27.56150677383524 82-83 27.85855302625408 22.88805750373578 21.430830815545203 27.82255865446494 84-85 28.30921033923849 22.090311940171183 20.92728978872238 28.673187931867943 86-87 28.78320565117899 21.62570888468809 20.286538652870362 29.30454681126256 88-89 29.077347170423216 20.830225393221987 19.374087887141236 30.718339549213557 90-91 23.461294416243657 23.318527918781726 21.399111675126903 31.821065989847714 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 2.0 9 2.0 10 2.0 11 5.0 12 9.0 13 13.0 14 19.0 15 34.5 16 54.0 17 70.0 18 99.5 19 146.0 20 192.5 21 272.0 22 383.0 23 489.0 24 618.0 25 815.5 26 1067.0 27 1370.0 28 1753.5 29 2172.5 30 2682.5 31 3317.5 32 3995.5 33 4718.0 34 5655.5 35 6798.5 36 8101.5 37 9516.0 38 11017.5 39 12693.0 40 14598.5 41 16518.0 42 18225.5 43 20108.0 44 22198.0 45 24046.0 46 25744.5 47 27394.0 48 28663.5 49 29607.5 50 30542.0 51 31455.5 52 32159.5 53 32601.5 54 32746.0 55 32682.5 56 32545.0 57 32309.0 58 32028.5 59 31548.0 60 30939.5 61 30304.0 62 29356.0 63 27936.0 64 26084.0 65 24086.0 66 21994.0 67 19999.0 68 17857.5 69 15570.5 70 13564.5 71 11559.0 72 9465.5 73 7482.0 74 5830.0 75 4662.5 76 3676.0 77 2716.0 78 2012.0 79 1478.0 80 1061.0 81 753.0 82 515.0 83 333.5 84 212.5 85 143.5 86 87.5 87 61.5 88 51.5 89 35.0 90 18.5 91 11.0 92 11.0 93 8.5 94 5.5 95 4.0 96 2.5 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0012144913103146748 2 0.007894193517045385 3 0.0010120760919288957 4 0.0 5 0.0 6 2.024152183857791E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 7504.0 32-33 16116.0 34-35 17253.0 36-37 18427.0 38-39 19402.0 40-41 20291.0 42-43 20717.0 44-45 20918.0 46-47 21254.0 48-49 20968.0 50-51 21018.0 52-53 20597.0 54-55 20094.0 56-57 19625.0 58-59 18769.0 60-61 18122.0 62-63 17582.0 64-65 16925.0 66-67 16207.0 68-69 15217.0 70-71 14660.0 72-73 14135.0 74-75 13513.0 76-77 12599.0 78-79 12083.0 80-81 11430.0 82-83 10858.0 84-85 10174.0 86-87 9829.0 88-89 9250.0 90-91 8497.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.77828887179399 #Duplication Level Percentage of deduplicated Percentage of total 1 99.78677234165568 99.56553396289664 2 0.2062133573116185 0.41151231870122285 3 0.005351994760492406 0.016020386377582175 4 0.001362793210712039 0.0054390869860378175 5 2.995130614955807E-4 0.0014942450385390725 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.072456551573373E-4 0.0 0.0 0.0 0.0 2 6.072456551573373E-4 0.0 2.024152183857791E-4 0.0 0.0 3 6.072456551573373E-4 0.0 2.024152183857791E-4 0.0 0.0 4 6.072456551573373E-4 0.0 2.024152183857791E-4 0.0 0.0 5 6.072456551573373E-4 0.0 2.024152183857791E-4 0.0 0.0 6 6.072456551573373E-4 0.0 2.024152183857791E-4 0.0 0.0 7 0.0012144913103146746 0.0 2.024152183857791E-4 0.0 0.0 8 0.005060380459644478 0.0 2.024152183857791E-4 0.0 0.0 9 0.005465210896416036 0.0 2.024152183857791E-4 0.0 0.0 10-11 0.005465210896416036 0.0 2.024152183857791E-4 0.0 0.0 12-13 0.005465210896416036 0.0 2.024152183857791E-4 0.0 0.0 14-15 0.005465210896416036 3.0362282757866865E-4 2.024152183857791E-4 0.0 0.0 16-17 0.005465210896416036 4.048304367715582E-4 2.024152183857791E-4 0.0 0.0 18-19 0.005768833723994705 4.048304367715582E-4 2.024152183857791E-4 0.0 0.0 20-21 0.005870041333187594 4.048304367715582E-4 2.024152183857791E-4 0.0 0.0 22-23 0.006274871769959152 4.048304367715582E-4 2.024152183857791E-4 0.0 0.0 24-25 0.006274871769959152 4.048304367715582E-4 2.024152183857791E-4 0.0 0.0 26-27 0.006376079379152042 4.048304367715582E-4 2.024152183857791E-4 0.0 1.0120760919288956E-4 28-29 0.006578494597537821 4.048304367715582E-4 2.024152183857791E-4 0.0 2.024152183857791E-4 30-31 0.006679702206730711 4.048304367715582E-4 2.024152183857791E-4 0.0 2.024152183857791E-4 32-33 0.007286947861888048 4.048304367715582E-4 2.024152183857791E-4 0.0 3.0362282757866865E-4 34-35 0.007792985907852495 4.048304367715582E-4 2.024152183857791E-4 0.0 4.048304367715582E-4 36-37 0.008096608735431164 4.048304367715582E-4 2.024152183857791E-4 0.0 4.048304367715582E-4 38-39 0.008400231563009833 4.048304367715582E-4 2.024152183857791E-4 0.0 4.048304367715582E-4 40-41 0.00890626960897428 4.048304367715582E-4 2.024152183857791E-4 0.0 4.048304367715582E-4 42-43 0.00910868482736006 4.048304367715582E-4 2.024152183857791E-4 0.0 4.048304367715582E-4 44-45 0.00910868482736006 4.048304367715582E-4 4.048304367715582E-4 0.0 4.048304367715582E-4 46-47 0.009412307654938728 4.048304367715582E-4 4.048304367715582E-4 0.0 4.048304367715582E-4 48-49 0.009817138091710286 4.048304367715582E-4 4.048304367715582E-4 0.0 4.048304367715582E-4 50-51 0.010221968528481845 4.048304367715582E-4 4.048304367715582E-4 0.0 4.048304367715582E-4 52-53 0.010323176137674734 4.048304367715582E-4 4.048304367715582E-4 0.0 6.072456551573373E-4 54-55 0.010323176137674734 4.048304367715582E-4 4.048304367715582E-4 0.0 6.072456551573373E-4 56-57 0.010323176137674734 4.048304367715582E-4 4.048304367715582E-4 0.0 6.072456551573373E-4 58-59 0.010424383746867624 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8.096608735431164E-4 80 0.010930421792832072 4.048304367715582E-4 4.048304367715582E-4 0.0 8.096608735431164E-4 >>END_MODULE