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"TTCTC":90879, "TTCTG":78777, "TTCCA":84001, "TTCCT":102305, "TTCCC":73625, "TTCCG":69073, "TTCGA":61053, "TTCGT":53390, "TTCGC":66131, "TTCGG":69664, "TTGAA":73320, "TTGAT":77073, "TTGAC":53659, "TTGAG":62367, "TTGTA":33846, "TTGTT":79497, "TTGTC":59349, "TTGTG":50735, "TTGCA":47927, "TTGCT":68514, "TTGCC":84871, "TTGCG":53667, "TTGGA":64855, "TTGGT":76116, "TTGGC":86944, "TTGGG":79330, "TCAAA":68266, "TCAAT":52465, "TCAAC":69680, "TCAAG":73094, "TCATA":34784, "TCATT":48339, "TCATC":85974, "TCATG":51204, "TCACA":37050, "TCACT":31100, "TCACC":132133, "TCACG":46228, "TCAGA":66267, "TCAGT":33858, "TCAGC":75383, "TCAGG":63045, "TCTAA":23960, "TCTAT":36112, "TCTAC":40487, "TCTAG":26450, "TCTTA":27914, "TCTTT":92270, "TCTTC":111414, "TCTTG":72654, "TCTCA":44002, "TCTCT":60441, "TCTCC":96444, "TCTCG":56121, "TCTGA":231440, "TCTGT":38498, "TCTGC":70011, "TCTGG":82249, "TCCAA":67530, "TCCAT":62315, "TCCAC":71760, "TCCAG":195734, "TCCTA":31179, "TCCTT":84958, "TCCTC":111967, "TCCTG":108130, 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"CACGA":54466, "CACGT":285321, "CACGC":58068, "CACGG":58029, "CAGAA":81301, "CAGAT":186454, "CAGAC":50699, "CAGAG":58220, "CAGTA":32129, "CAGTT":55824, "CAGTC":114293, "CAGTG":36004, "CAGCA":117083, "CAGCT":65260, "CAGCC":113552, "CAGCG":82537, "CAGGA":109890, "CAGGT":64340, "CAGGC":96962, "CAGGG":72432, "CTAAA":32142, "CTAAT":20534, "CTAAC":21160, "CTAAG":27441, "CTATA":22586, "CTATT":36561, "CTATC":41198, "CTATG":34095, "CTACA":36247, "CTACT":30752, "CTACC":53379, "CTACG":35905, "CTAGA":58085, "CTAGT":14664, "CTAGC":20758, "CTAGG":42537, "CTTAA":27363, "CTTAT":32257, "CTTAC":24635, "CTTAG":32664, "CTTTA":43506, "CTTTT":92829, "CTTTC":82015, "CTTTG":71140, "CTTCA":91895, "CTTCT":104860, "CTTCC":95032, "CTTCG":81871, "CTTGA":67931, "CTTGT":62011, "CTTGC":68527, "CTTGG":77118, "CTCAA":64732, "CTCAT":48644, "CTCAC":43909, "CTCAG":53957, "CTCTA":33327, "CTCTT":69281, "CTCTC":54116, "CTCTG":53601, "CTCCA":195958, "CTCCT":112472, "CTCCC":78468, "CTCCG":66661, "CTCGA":63762, "CTCGT":47422, "CTCGC":59807, "CTCGG":63106, "CTGAA":239962, "CTGAT":57293, "CTGAC":48086, "CTGAG":53890, "CTGTA":28578, "CTGTT":71378, "CTGTC":45503, "CTGTG":44692, "CTGCA":72368, "CTGCT":108897, "CTGCC":90972, "CTGCG":67784, "CTGGA":92851, "CTGGT":83258, "CTGGC":101700, "CTGGG":93283, "CCAAA":102024, "CCAAT":54403, "CCAAC":103874, "CCAAG":97821, "CCATA":46052, "CCATT":55932, "CCATC":90419, "CCATG":68514, "CCACA":83273, "CCACT":46707, "CCACC":145344, "CCACG":70497, "CCAGA":135102, "CCAGT":144265, "CCAGC":139508, "CCAGG":99422, "CCTAA":29230, "CCTAT":33419, "CCTAC":41392, "CCTAG":61939, "CCTTA":31742, "CCTTT":75595, "CCTTC":100687, "CCTTG":82378, "CCTCA":71062, "CCTCT":63844, "CCTCC":122588, "CCTCG":75121, "CCTGA":69087, "CCTGT":64928, "CCTGC":122502, "CCTGG":97356, "CCCAA":103233, "CCCAT":61861, "CCCAC":93597, "CCCAG":110601, "CCCTA":36959, "CCCTT":62077, "CCCTC":73812, "CCCTG":77186, "CCCCA":105966, "CCCCT":67049, "CCCCC":76361, "CCCCG":77039, "CCCGA":67541, "CCCGT":49548, "CCCGC":94886, "CCCGG":76231, "CCGAA":75460, "CCGAT":64926, "CCGAC":69068, "CCGAG":74510, "CCGTA":37428, "CCGTT":48968, "CCGTC":68551, "CCGTG":53609, "CCGCA":80721, "CCGCT":71857, "CCGCC":147764, "CCGCG":77604, "CCGGA":70770, "CCGGT":65587, "CCGGC":111421, "CCGGG":73615, "CGAAA":69390, "CGAAT":44302, "CGAAC":58273, "CGAAG":92421, "CGATA":45727, "CGATT":50451, "CGATC":65242, "CGATG":81380, "CGACA":61079, "CGACT":45569, "CGACC":75407, "CGACG":75366, "CGAGA":84402, "CGAGT":33516, "CGAGC":63161, "CGAGG":73122, "CGTAA":31959, "CGTAT":25993, "CGTAC":24439, "CGTAG":42612, "CGTTA":22997, "CGTTT":48432, "CGTTC":51855, "CGTTG":54152, "CGTCA":54793, "CGTCT":265373, "CGTCC":54717, "CGTCG":71491, "CGTGA":46504, "CGTGT":28677, "CGTGC":47853, "CGTGG":61131, "CGCAA":60410, "CGCAT":44330, "CGCAC":50869, "CGCAG":78942, "CGCTA":28676, "CGCTT":62118, "CGCTC":58753, "CGCTG":83905, "CGCCA":113321, "CGCCT":89552, "CGCCC":99131, "CGCCG":133288, "CGCGA":58434, "CGCGT":39523, "CGCGC":83130, "CGCGG":75836, "CGGAA":480095, "CGGAT":52936, "CGGAC":41383, "CGGAG":74383, "CGGTA":40948, "CGGTT":55993, "CGGTC":58196, "CGGTG":77147, "CGGCA":93648, "CGGCT":83565, "CGGCC":101418, "CGGCG":129412, "CGGGA":61967, "CGGGT":55533, "CGGGC":76694, "CGGGG":70504, "GAAAA":109696, "GAAAT":70622, "GAAAC":69114, "GAAAG":86969, "GAATA":44169, "GAATT":53454, "GAATC":53838, "GAATG":48000, "GAACA":72254, "GAACT":199337, "GAACC":69735, "GAACG":50759, "GAAGA":525010, "GAAGT":59356, "GAAGC":93751, "GAAGG":94264, "GATAA":50137, "GATAT":42614, "GATAC":34609, "GATAG":52644, "GATTA":35475, "GATTT":71401, "GATTC":56028, "GATTG":55269, "GATCA":66983, "GATCT":64168, "GATCC":60770, "GATCG":523041, "GATGA":90531, "GATGT":52493, "GATGC":67913, "GATGG":84582, "GACAA":63305, "GACAT":46415, "GACAC":36347, "GACAG":54567, "GACTA":26588, "GACTT":49675, "GACTC":33049, "GACTG":42043, "GACCA":75237, "GACCT":56630, "GACCC":61532, "GACCG":61429, "GACGA":75399, "GACGT":38538, "GACGC":54925, "GACGG":63331, "GAGAA":92089, "GAGAT":158952, "GAGAC":54091, "GAGAG":59209, "GAGTA":26081, "GAGTT":47499, "GAGTC":29852, "GAGTG":29475, "GAGCA":406132, "GAGCT":50168, "GAGCC":61138, "GAGCG":55785, "GAGGA":109314, "GAGGT":60696, "GAGGC":74679, "GAGGG":63696, "GTAAA":32486, "GTAAT":27068, "GTAAC":28414, "GTAAG":26483, "GTATA":18443, "GTATT":34083, "GTATC":30911, "GTATG":27324, "GTACA":27512, "GTACT":23873, "GTACC":30574, "GTACG":24421, "GTAGA":76725, "GTAGT":28004, "GTAGC":38107, "GTAGG":35685, "GTTAA":24006, "GTTAT":30846, "GTTAC":28597, "GTTAG":28894, "GTTTA":32417, "GTTTT":79170, "GTTTC":66855, "GTTTG":69551, "GTTCA":56985, "GTTCT":60193, "GTTCC":72786, "GTTCG":54689, "GTTGA":77461, "GTTGT":60643, "GTTGC":63086, "GTTGG":86419, "GTCAA":53823, "GTCAT":44535, "GTCAC":92280, "GTCAG":51887, "GTCTA":27587, "GTCTT":58100, "GTCTC":50468, "GTCTG":248513, "GTCCA":55430, "GTCCT":50727, "GTCCC":43655, "GTCCG":41143, "GTCGA":64264, "GTCGT":51059, "GTCGC":59279, "GTCGG":61222, "GTGAA":63413, "GTGAT":55239, "GTGAC":37087, "GTGAG":42256, "GTGTA":21886, "GTGTT":46384, "GTGTC":30052, "GTGTG":35228, "GTGCA":41185, "GTGCT":53340, "GTGCC":54817, "GTGCG":44985, "GTGGA":67629, "GTGGT":74706, "GTGGC":73355, "GTGGG":71770, "GCAAA":72241, "GCAAT":50683, "GCAAC":65949, "GCAAG":80406, "GCATA":35232, "GCATT":46795, "GCATC":63832, "GCATG":50277, "GCACA":343646, "GCACT":32434, "GCACC":79770, "GCACG":50795, "GCAGA":110513, "GCAGT":43392, "GCAGC":97176, "GCAGG":115362, "GCTAA":28082, "GCTAT":36630, "GCTAC":40090, "GCTAG":31968, "GCTTA":36008, "GCTTT":72694, "GCTTC":95032, "GCTTG":74609, "GCTCA":58640, "GCTCT":50364, "GCTCC":74838, "GCTCG":61979, "GCTGA":75855, "GCTGT":59423, "GCTGC":95622, "GCTGG":125706, "GCCAA":95317, "GCCAT":74601, "GCCAC":82650, "GCCAG":120214, "GCCTA":37778, "GCCTT":75494, "GCCTC":76158, "GCCTG":96312, "GCCCA":95947, "GCCCT":69283, "GCCCC":78256, "GCCCG":79756, "GCCGA":103625, "GCCGT":74369, "GCCGC":125404, "GCCGG":108707, "GCGAA":68583, "GCGAT":62521, "GCGAC":63437, "GCGAG":63547, "GCGTA":30266, "GCGTT":47323, "GCGTC":49899, "GCGTG":50681, "GCGCA":71883, "GCGCT":66457, "GCGCC":103140, "GCGCG":80834, "GCGGA":72591, "GCGGT":70082, "GCGGC":118317, "GCGGG":85538, "GGAAA":90391, "GGAAT":57887, "GGAAC":69648, "GGAAG":496060, "GGATA":51563, "GGATT":62826, "GGATC":54949, "GGATG":77563, "GGACA":52253, "GGACT":38301, "GGACC":46221, "GGACG":55503, "GGAGA":138700, "GGAGT":41920, "GGAGC":71868, "GGAGG":112580, "GGTAA":34811, "GGTAT":34902, "GGTAC":29950, "GGTAG":61016, "GGTTA":34378, "GGTTT":83457, "GGTTC":63957, "GGTTG":103770, "GGTCA":58932, "GGTCT":58102, "GGTCC":43781, "GGTCG":64229, "GGTGA":83426, "GGTGT":53742, "GGTGC":70497, "GGTGG":113978, "GGCAA":86300, "GGCAT":64640, "GGCAC":55951, "GGCAG":100513, "GGCTA":46564, "GGCTT":87539, "GGCTC":59714, "GGCTG":103293, "GGCCA":97629, "GGCCT":70312, "GGCCC":65612, "GGCCG":99958, "GGCGA":105243, "GGCGT":67007, "GGCGC":102899, "GGCGG":133439, "GGGAA":67896, "GGGAT":59918, "GGGAC":43930, "GGGAG":84336, "GGGTA":35737, "GGGTT":78528, "GGGTC":51753, "GGGTG":76232, "GGGCA":77219, "GGGCT":67471, "GGGCC":63286, "GGGCG":89331, "GGGGA":73963, "GGGGT":69674, "GGGGC":68492, "GGGGG":120257 }, "overrepresented_sequences": { } }, "read2_before_filtering": { "total_reads": 1197982, "total_bases": 73076902, "q20_bases": 71180358, "q30_bases": 68059936, "total_cycles": 61, "quality_curves": { "A":[39.353,39.1536,38.9581,38.7967,39.0256,39.1126,39.0941,38.8712,39.0859,39.074,39.1025,39.0728,39.1048,39.1148,39.094,39.089,39.1134,39.094,39.1151,39.0861,38.6894,39.067,39.0931,39.097,38.7191,39.0793,39.0752,38.6697,38.9894,38.8171,39.0787,39.0755,39.0273,38.8031,38.9201,38.9914,39.0055,38.9695,38.916,38.8691,38.7987,38.708,38.6311,38.5455,38.3918,38.185,37.8094,37.5421,37.7851,37.4887,37.2832,37.0409,36.9,36.4573,36.3269,36.0048,35.7266,35.5592,35.3495,34.8544,34.6677], 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"CCTGG":60813, "CCCAA":70461, "CCCAT":42879, "CCCAC":64585, "CCCAG":61653, "CCCTA":22829, "CCCTT":43203, "CCCTC":51671, "CCCTG":49832, "CCCCA":71033, "CCCCT":47399, "CCCCC":50928, "CCCCG":51174, "CCCGA":42092, "CCCGT":31066, "CCCGC":62470, "CCCGG":50168, "CCGAA":45981, "CCGAT":42032, "CCGAC":43997, "CCGAG":42193, "CCGTA":21361, "CCGTT":33603, "CCGTC":42436, "CCGTG":32439, "CCGCA":47696, "CCGCT":47682, "CCGCC":96786, "CCGCG":48062, "CCGGA":44862, "CCGGT":43254, "CCGGC":70065, "CCGGG":47007, "CGAAA":44985, "CGAAT":29907, "CGAAC":35538, "CGAAG":50599, "CGATA":27213, "CGATT":35293, "CGATC":42724, "CGATG":50632, "CGACA":36038, "CGACT":30017, "CGACC":49320, "CGACG":46804, "CGAGA":38835, "CGAGT":21900, "CGAGC":38705, "CGAGG":45292, "CGTAA":19080, "CGTAT":16546, "CGTAC":15033, "CGTAG":20428, "CGTTA":13744, "CGTTT":33737, "CGTTC":34389, "CGTTG":35512, "CGTCA":31983, "CGTCT":31990, "CGTCC":36212, "CGTCG":44188, "CGTGA":27338, "CGTGT":17970, "CGTGC":27418, "CGTGG":37792, "CGCAA":35673, "CGCAT":27556, "CGCAC":30723, "CGCAG":41091, "CGCTA":16822, "CGCTT":40586, "CGCTC":37865, "CGCTG":50007, "CGCCA":65952, "CGCCT":59039, "CGCCC":62216, "CGCCG":81286, "CGCGA":34751, "CGCGT":23163, "CGCGC":46450, "CGCGG":46496, "CGGAA":42376, "CGGAT":34821, "CGGAC":25416, "CGGAG":41602, "CGGTA":23161, "CGGTT":39237, "CGGTC":38525, "CGGTG":48558, "CGGCA":53613, "CGGCT":55686, "CGGCC":61643, "CGGCG":77086, "CGGGA":38486, "CGGGT":36738, "CGGGC":46140, "CGGGG":44592, "GAAAA":77773, "GAAAT":48869, "GAAAC":47195, "GAAAG":52517, "GAATA":29437, "GAATT":38844, "GAATC":37660, "GAATG":32496, "GAACA":44588, "GAACT":36137, "GAACC":46479, "GAACG":32168, "GAAGA":73439, "GAAGT":37203, "GAAGC":60772, "GAAGG":60753, "GATAA":32476, "GATAT":29294, "GATAC":22592, "GATAG":26462, "GATTA":23809, "GATTT":53023, "GATTC":39318, "GATTG":37943, "GATCA":42306, "GATCT":40674, "GATCC":40163, "GATCG":40177, "GATGA":59946, "GATGT":34757, "GATGC":42221, "GATGG":55232, "GACAA":41133, "GACAT":29799, "GACAC":23001, "GACAG":28656, "GACTA":16250, "GACTT":34846, "GACTC":21422, "GACTG":25922, "GACCA":47584, "GACCT":36499, "GACCC":41387, "GACCG":40150, "GACGA":47077, "GACGT":23805, "GACGC":33982, "GACGG":39379, "GAGAA":62041, "GAGAT":42792, "GAGAC":36548, "GAGAG":35288, "GAGTA":15787, "GAGTT":32361, "GAGTC":20003, "GAGTG":19376, "GAGCA":46591, "GAGCT":31087, "GAGCC":39905, "GAGCG":33669, "GAGGA":73352, "GAGGT":38967, "GAGGC":47920, "GAGGG":41153, "GTAAA":21539, "GTAAT":18289, "GTAAC":17229, "GTAAG":14197, "GTATA":11518, "GTATT":23723, "GTATC":19687, "GTATG":18412, "GTACA":16137, "GTACT":15237, "GTACC":19449, "GTACG":15074, "GTAGA":24166, "GTAGT":18467, "GTAGC":23235, "GTAGG":22053, "GTTAA":15971, "GTTAT":21609, "GTTAC":19355, "GTTAG":13377, "GTTTA":21182, "GTTTT":58969, "GTTTC":47982, "GTTTG":50682, "GTTCA":36821, "GTTCT":43231, "GTTCC":50961, "GTTCG":35744, "GTTGA":44346, "GTTGT":44320, "GTTGC":42311, "GTTGG":62054, "GTCAA":33550, "GTCAT":29468, "GTCAC":24240, "GTCAG":27141, "GTCTA":16971, "GTCTT":40663, "GTCTC":36745, "GTCTG":31022, "GTCCA":35283, "GTCCT":35095, "GTCCC":30180, "GTCCG":25472, "GTCGA":38335, "GTCGT":32550, "GTCGC":37111, "GTCGG":38510, "GTGAA":37074, "GTGAT":34765, "GTGAC":22488, "GTGAG":24700, "GTGTA":12285, "GTGTT":32460, "GTGTC":19314, "GTGTG":23544, "GTGCA":24361, "GTGCT":34219, "GTGCC":33598, "GTGCG":26932, "GTGGA":41765, "GTGGT":51661, "GTGGC":46484, "GTGGG":47722, "GCAAA":44738, "GCAAT":33716, "GCAAC":43103, "GCAAG":41433, "GCATA":21465, "GCATT":31782, "GCATC":40153, "GCATG":30942, "GCACA":27262, "GCACT":20601, "GCACC":51565, "GCACG":29413, "GCAGA":42985, "GCAGT":28384, "GCAGC":60210, "GCAGG":71599, "GCTAA":16744, "GCTAT":23737, "GCTAC":24943, "GCTAG":13398, "GCTTA":20072, "GCTTT":51269, "GCTTC":66400, "GCTTG":49989, "GCTCA":37009, "GCTCT":34600, "GCTCC":51980, "GCTCG":39781, "GCTGA":46673, "GCTGT":37637, "GCTGC":60276, "GCTGG":79627, "GCCAA":59274, "GCCAT":46241, "GCCAC":52553, "GCCAG":59670, "GCCTA":22610, "GCCTT":50597, "GCCTC":53217, "GCCTG":60889, "GCCCA":58956, "GCCCT":44954, "GCCCC":51412, "GCCCG":48882, "GCCGA":61190, "GCCGT":44857, "GCCGC":75932, "GCCGG":65915, "GCGAA":40369, "GCGAT":39709, "GCGAC":38520, "GCGAG":34629, "GCGTA":16383, "GCGTT":30171, "GCGTC":30616, "GCGTG":28833, "GCGCA":38251, "GCGCT":38659, "GCGCC":59477, "GCGCG":44024, "GCGGA":43393, "GCGGT":45875, "GCGGC":71250, "GCGGG":53518, "GGAAA":59035, "GGAAT":41006, "GGAAC":45801, "GGAAG":56748, "GGATA":31249, "GGATT":44699, "GGATC":37215, "GGATG":50492, "GGACA":32025, "GGACT":25115, "GGACC":30157, "GGACG":33593, "GGAGA":67019, "GGAGT":27670, "GGAGC":45912, "GGAGG":74983, "GGTAA":21212, "GGTAT":23377, "GGTAC":18746, "GGTAG":28120, "GGTTA":20729, "GGTTT":61352, "GGTTC":44786, "GGTTG":65689, "GGTCA":35850, "GGTCT":40755, "GGTCC":29028, "GGTCG":41023, "GGTGA":49839, "GGTGT":36868, "GGTGC":44103, "GGTGG":76897, "GGCAA":51594, "GGCAT":40894, "GGCAC":34027, "GGCAG":52788, "GGCTA":27059, "GGCTT":58592, "GGCTC":40351, "GGCTG":66731, "GGCCA":54387, "GGCCT":45280, "GGCCC":41069, "GGCCG":58329, "GGCGA":62636, "GGCGT":40259, "GGCGC":56481, "GGCGG":83593, "GGGAA":42133, "GGGAT":39990, "GGGAC":27327, "GGGAG":46700, "GGGTA":20784, "GGGTT":56463, "GGGTC":34756, "GGGTG":49168, "GGGCA":45646, "GGGCT":44901, "GGGCC":38767, "GGGCG":52112, "GGGGA":45437, "GGGGT":46521, "GGGGC":44096, "GGGGG":37494 }, "overrepresented_sequences": { } }, "command": "/redser4/software/fastp -l 30 -q 25 -y -Y 30 --detect_adapter_for_pe -i /scratch3/cwanket/andy-lake/raw/AM-AL-8C_S70_R1_001.fastq.gz -I /scratch3/cwanket/andy-lake/raw/AM-AL-8C_S70_R2_001.fastq.gz -o AM-AL-8C.trim.R1.fastq.gz -O AM-AL-8C.trim.R2.fastq.gz -m --merged_out AM-AL-8C.trim.merged.fastq.gz " }