##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-8B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 300794 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.255902710825346 40.0 40.0 40.0 40.0 40.0 2 39.14054801625032 40.0 40.0 40.0 40.0 40.0 3 39.10489570935591 40.0 40.0 40.0 40.0 40.0 4 39.07369162948729 40.0 40.0 40.0 40.0 40.0 5 39.20603469484099 40.0 40.0 40.0 40.0 40.0 6 39.2611687733133 40.0 40.0 40.0 40.0 40.0 7 39.286993756524396 40.0 40.0 40.0 40.0 40.0 8 39.32135614407202 40.0 40.0 40.0 40.0 40.0 9 39.33043877204997 40.0 40.0 40.0 40.0 40.0 10-11 39.328803101125686 40.0 40.0 40.0 40.0 40.0 12-13 39.32216068139657 40.0 40.0 40.0 40.0 40.0 14-15 39.329188747115964 40.0 40.0 40.0 40.0 40.0 16-17 39.47250277598622 40.0 40.0 40.0 40.0 40.0 18-19 39.47909865223376 40.0 40.0 40.0 40.0 40.0 20-21 39.48583415892604 40.0 40.0 40.0 40.0 40.0 22-23 39.49116006303317 40.0 40.0 40.0 40.0 40.0 24-25 39.49287552278304 40.0 40.0 40.0 40.0 40.0 26-27 39.47345359282433 40.0 40.0 40.0 40.0 40.0 28-29 39.47611322034349 40.0 40.0 40.0 40.0 40.0 30-31 39.46034283854668 40.0 40.0 40.0 40.0 40.0 32-33 39.459360859363734 40.0 40.0 40.0 40.0 40.0 34-35 39.46675366835083 40.0 40.0 40.0 40.0 40.0 36-37 39.45501938729437 40.0 40.0 40.0 40.0 40.0 38-39 39.44490022787563 40.0 40.0 40.0 40.0 40.0 40-41 39.426076692862026 40.0 40.0 40.0 40.0 40.0 42-43 39.41288236872397 40.0 40.0 40.0 40.0 40.0 44-45 39.4062036125895 40.0 40.0 40.0 40.0 40.0 46-47 39.39091340646655 40.0 40.0 40.0 40.0 40.0 48-49 39.396599892184774 40.0 40.0 40.0 40.0 40.0 50-51 39.39051287182264 40.0 40.0 40.0 40.0 40.0 52-53 39.390988271831915 40.0 40.0 40.0 40.0 40.0 54-55 39.34929072526616 40.0 40.0 40.0 40.0 40.0 56-57 39.36502079145921 40.0 40.0 40.0 40.0 40.0 58-59 39.35687118130839 40.0 40.0 40.0 40.0 40.0 60-61 39.31342505713989 40.0 40.0 40.0 40.0 40.0 62-63 39.362332563916084 40.0 40.0 40.0 40.0 40.0 64-65 39.354315267360974 40.0 40.0 40.0 40.0 40.0 66-67 39.34026951919289 40.0 40.0 40.0 40.0 40.0 68-69 39.34624109553491 40.0 40.0 40.0 40.0 40.0 70-71 39.35551497744032 40.0 40.0 40.0 40.0 40.0 72-73 39.33140882824153 40.0 40.0 40.0 40.0 40.0 74-75 39.323885947575036 40.0 40.0 40.0 40.0 40.0 76-77 39.31306784102074 40.0 40.0 40.0 40.0 40.0 78-79 39.29616823456016 40.0 40.0 40.0 40.0 40.0 80-81 39.29537150238433 40.0 40.0 40.0 40.0 40.0 82-83 39.31089842312142 40.0 40.0 40.0 40.0 40.0 84-85 39.29797065079177 40.0 40.0 40.0 40.0 40.0 86-87 39.28099970909889 40.0 40.0 40.0 40.0 40.0 88-89 39.314779220517465 40.0 40.0 40.0 40.0 40.0 90-91 39.43623543838136 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15071396427305928 1101 2 0.010348414441523346 1101 3 -0.02226278534552506 1101 4 0.16790888984248653 1101 5 0.2110773231907075 1101 6 0.07408593487888027 1101 7 0.10204087965428954 1101 8 0.0028020871398979352 1101 9 0.12071300185861844 1101 10-11 -0.10189324358377405 1101 12-13 0.1395291218346344 1101 14-15 0.11129567463918022 1101 16-17 0.10802076351712486 1101 18-19 0.12611584713982893 1101 20-21 0.18759346011707123 1101 22-23 0.10157148464193 1101 24-25 0.13544824063055216 1101 26-27 0.22130775118913704 1101 28-29 0.07902300197323342 1101 30-31 0.2135803844514257 1101 32-33 0.08488860475892324 1101 34-35 0.1997628301654828 1101 36-37 0.13199838258944396 1101 38-39 0.14509112462734208 1101 40-41 0.09170990677471025 1101 42-43 0.16709229936429892 1101 44-45 0.1456646043676031 1101 46-47 0.09981787308895917 1101 48-49 0.26165562339600257 1101 50-51 0.20478696675817076 1101 52-53 0.09490776631149345 1101 54-55 0.09354552299762275 1101 56-57 0.19566254622701962 1101 58-59 0.13465333465826745 1101 60-61 0.2251919758895582 1101 62-63 0.2652015292748402 1101 64-65 0.08856142463913841 1101 66-67 0.23599315719510372 1101 68-69 0.16012438652442285 1101 70-71 0.11987305983591767 1101 72-73 -0.15564081234232674 1101 74-75 0.14361015701948787 1101 76-77 0.21270267876437998 1101 78-79 0.34199557402465786 1101 80-81 0.33893951883523243 1101 82-83 0.2469438009310494 1101 84-85 0.14152936546086892 1101 86-87 0.5436544820198677 1101 88-89 0.6861082842402197 1101 90-91 0.8375272287800968 1102 1 0.2939892202718326 1102 2 0.378757401144874 1102 3 0.022353598576223987 1102 4 0.1750233633255931 1102 5 -0.16834343181464817 1102 6 -0.013190543800625676 1102 7 0.1241637590674074 1102 8 -0.1834045459030449 1102 9 0.03258064666986371 1102 10-11 0.02399702082093569 1102 12-13 -0.07544489619990458 1102 14-15 -0.15584095993211378 1102 16-17 0.13684546166781075 1102 18-19 -0.057076113734389367 1102 20-21 -0.1367034984646196 1102 22-23 0.03122531316095234 1102 24-25 -0.04809142007554357 1102 26-27 -0.0728080978097978 1102 28-29 -0.09292411708530324 1102 30-31 0.018528420520510736 1102 32-33 -0.08835884710003228 1102 34-35 -0.06472507955051299 1102 36-37 -0.14140182073576568 1102 38-39 -0.06237907984547064 1102 40-41 0.13577922992848102 1102 42-43 -0.12259115114089525 1102 44-45 -0.12272028184973749 1102 46-47 0.005569918188250256 1102 48-49 -0.0835039452209898 1102 50-51 -0.0700982887072712 1102 52-53 0.0474640082563198 1102 54-55 -0.006376749982244689 1102 56-57 -0.022329781144165395 1102 58-59 -0.07624905999966813 1102 60-61 0.012640623624690761 1102 62-63 -0.18433522145611647 1102 64-65 0.14091573190663098 1102 66-67 0.040986322064249237 1102 68-69 -0.07648359649409286 1102 70-71 -0.010359168994440893 1102 72-73 -0.11040028836593052 1102 74-75 -0.12924018166782503 1102 76-77 0.21281773005360805 1102 78-79 -0.03208847605474574 1102 80-81 0.32462966242010793 1102 82-83 0.16263664245832388 1102 84-85 -0.21940453310743635 1102 86-87 -0.5155445167285748 1102 88-89 0.1361082842402226 1102 90-91 0.8375272287800968 1103 1 0.28017241431974327 1103 2 0.13219715393732656 1103 3 0.16889034257231117 1103 4 -0.062389896338650885 1103 5 0.05482148752310678 1103 6 0.10223719538308274 1103 7 -0.042917103538982815 1103 8 -0.14841173172097655 1103 9 -0.10248961252046485 1103 10-11 0.009124496845736019 1103 12-13 -0.044201036895515244 1103 14-15 -0.2069816362851853 1103 16-17 0.07366035268612592 1103 18-19 0.03589642603805743 1103 20-21 -0.09240187134884792 1103 22-23 -0.016705826282439773 1103 24-25 -0.10012598906131842 1103 26-27 -0.039009709128322356 1103 28-29 -0.14866140512293669 1103 30-31 -0.24844109080534338 1103 32-33 -0.19298062981170006 1103 34-35 -0.11319336991052609 1103 36-37 -0.23728687137190008 1103 38-39 -0.09792815117167208 1103 40-41 -0.017686197167336104 1103 42-43 -0.18795337499048514 1103 44-45 -0.12530772088611997 1103 46-47 -0.14412158401619735 1103 48-49 -0.05592587775528557 1103 50-51 -0.2874256258845591 1103 52-53 -0.18799689220292493 1103 54-55 -0.1916910956697322 1103 56-57 -0.027746456804116804 1103 58-59 -0.1428560726584749 1103 60-61 -0.34814265607866446 1103 62-63 0.16474125296442566 1103 64-65 -0.02164213613798438 1103 66-67 -0.00831757192955962 1103 68-69 -0.22115876507363907 1103 70-71 0.140375455585243 1103 72-73 -0.20615603744830224 1103 74-75 0.09767734879014967 1103 76-77 -0.11341111764986067 1103 78-79 0.03213320974935385 1103 80-81 0.11325013239651582 1103 82-83 0.0464229119947106 1103 84-85 0.16410953121312133 1103 86-87 0.08533940663482298 1103 88-89 0.28610828424022117 1103 90-91 -1.5780571868043225 1104 1 0.3897207429350047 1104 2 0.2070769732390474 1104 3 0.36667768031907855 1104 4 0.28610812355896087 1104 5 -8.442801102077624E-4 1104 6 0.31029283143060127 1104 7 0.06998959760301204 1104 8 -0.07348733225296655 1104 9 0.15662517397768738 1104 10-11 -7.007214776422188E-6 1104 12-13 -0.10445113162838027 1104 14-15 0.13306844210160307 1104 16-17 0.04483184515579097 1104 18-19 -0.07322839099750666 1104 20-21 -0.010203474748834651 1104 22-23 -0.07099703878866848 1104 24-25 0.06444912480385057 1104 26-27 -0.07235567350877403 1104 28-29 0.06382995746984221 1104 30-31 0.020837973605566162 1104 32-33 -0.03503766597526692 1104 34-35 -0.06691048800057331 1104 36-37 0.027547834359737067 1104 38-39 -0.07220055829440497 1104 40-41 0.07637411844502395 1104 42-43 0.06197178152189764 1104 44-45 0.0845642238330484 1104 46-47 -0.07590993119469402 1104 48-49 -0.04444066415928205 1104 50-51 -0.06969136159975875 1104 52-53 -0.14066867179328568 1104 54-55 -0.1435678288395863 1104 56-57 -0.23582060462032217 1104 58-59 -0.1296888865423682 1104 60-61 -0.09273870735552947 1104 62-63 -0.06736363340134943 1104 64-65 0.09647907970945369 1104 66-67 -0.035925391860295974 1104 68-69 0.07427558405404255 1104 70-71 0.022384483749206652 1104 72-73 0.0018640739925999128 1104 74-75 -0.37306429524091556 1104 76-77 -0.8984913220400088 1104 78-79 -0.550382957566022 1104 80-81 -0.15861168829111705 1104 82-83 0.16728693818595275 1104 84-85 0.1088254192359841 1104 86-87 -0.1763766544617127 1104 88-89 -0.1287065305745898 1104 90-91 0.8375272287800968 1201 1 0.29720056945531326 1201 2 0.17965012722149964 1201 3 0.20152948518587266 1201 4 0.08993348360620956 1201 5 0.2732424527471409 1201 6 0.0916748676851995 1201 7 0.1425547136463834 1201 8 0.17379462117590805 1201 9 -0.008557968716701225 1201 10-11 -0.06662754310067953 1201 12-13 -0.025567496522853617 1201 14-15 -5.290947942881985E-4 1201 16-17 -0.07581762032126704 1201 18-19 -0.02183101276354904 1201 20-21 -0.025241410326493963 1201 22-23 0.001340918107011646 1201 24-25 -0.17509194382267168 1201 26-27 0.03439514688523104 1201 28-29 8.719259960727754E-4 1201 30-31 0.11640207966442517 1201 32-33 0.08031053953063605 1201 34-35 -0.018121904294226 1201 36-37 -0.023708638565359763 1201 38-39 0.0012314201760261767 1201 40-41 -0.1883677581216645 1201 42-43 0.07113303817431671 1201 44-45 0.02692106303219788 1201 46-47 -0.08226201710382242 1201 48-49 -0.02848676907830594 1201 50-51 -0.04832967768084018 1201 52-53 -0.019324195209492245 1201 54-55 0.0073375716503463195 1201 56-57 -0.17236655196101225 1201 58-59 0.07646187719986841 1201 60-61 -5.247071902232392E-4 1201 62-63 -0.040456992542999615 1201 64-65 -0.0018630850582468383 1201 66-67 -0.07853586532121426 1201 68-69 -0.1649902472041802 1201 70-71 0.24274885980560157 1201 72-73 0.15705704876001647 1201 74-75 0.1535553280955284 1201 76-77 0.35374913870484903 1201 78-79 0.1358045241368373 1201 80-81 -0.00799934667948321 1201 82-83 0.3926918827724819 1201 84-85 0.23581258877635491 1201 86-87 0.13101747363817395 1201 88-89 0.36610828424021946 1201 90-91 0.03752722878009962 1202 1 0.09580775936684915 1202 2 -0.07060396651085199 1202 3 -0.0482728863556261 1202 4 0.15874583067942893 1202 5 0.015911378024760836 1202 6 0.11629372708667063 1202 7 -0.10423617662276286 1202 8 -0.17245043811262661 1202 9 0.09481112595673835 1202 10-11 -0.05586149755202996 1202 12-13 -0.002642595337079001 1202 14-15 0.05555974390325247 1202 16-17 0.052320907817261286 1202 18-19 -0.08928819326420978 1202 20-21 -0.07737767985406663 1202 22-23 0.08652819459863537 1202 24-25 -0.025157819975511586 1202 26-27 0.0467045765859595 1202 28-29 0.06004753299776411 1202 30-31 0.07292381879486243 1202 32-33 -0.08340589082519756 1202 34-35 0.11584105932161037 1202 36-37 -0.08925020606405809 1202 38-39 -0.03507521440740646 1202 40-41 0.12655771845539476 1202 42-43 0.07652566379583448 1202 44-45 0.05660483968273411 1202 46-47 -0.08032715641129329 1202 48-49 0.03822353802434009 1202 50-51 0.12305107400969462 1202 52-53 0.14860348682308455 1202 54-55 -0.06807848319076726 1202 56-57 -0.062054203619481996 1202 58-59 -0.04328312411901436 1202 60-61 -0.040551235341524716 1202 62-63 0.09370321834362727 1202 64-65 0.013721510602671572 1202 66-67 -0.1839074635713729 1202 68-69 -0.12647344787000492 1202 70-71 0.005563956249076796 1202 72-73 0.06557970776811572 1202 74-75 -0.11493066485903825 1202 76-77 -0.10270572667513278 1202 78-79 -0.2969621564333451 1202 80-81 -0.17269429739755537 1202 82-83 -0.49704096164867906 1202 84-85 -0.21475486179109993 1202 86-87 0.16434413719228047 1202 88-89 0.6861082842402197 1202 90-91 0.17086056211342537 1203 1 0.2704917420508366 1203 2 0.2303484144415222 1203 3 0.07051499243225123 1203 4 0.005464445398040141 1203 5 0.005521767635151775 1203 6 0.07341926821221278 1203 7 0.008040879654295452 1203 8 0.1519131982510089 1203 9 0.047157446303060624 1203 10-11 -0.11033768802821697 1203 12-13 0.051418010723523366 1203 14-15 -0.13637099202748715 1203 16-17 0.17290965240601253 1203 18-19 0.10756029158427083 1203 20-21 0.016371237894844626 1203 22-23 0.0032381513085937286 1203 24-25 0.1554482406305553 1203 26-27 -9.144710330843964E-4 1203 28-29 0.1201341130843403 1203 30-31 0.04213594000698606 1203 32-33 0.10444071380549502 1203 34-35 0.19298318621932253 1203 36-37 -0.03193423293072328 1203 38-39 0.06105878273460519 1203 40-41 0.004612960943980227 1203 42-43 -0.0019264478862837109 1203 44-45 0.054735876943276196 1203 46-47 0.015214379684927337 1203 48-49 0.09294100138349393 1203 50-51 -0.16609540876965667 1203 52-53 0.1176230231931541 1203 54-55 0.020000780110173366 1203 56-57 0.07743379410445783 1203 58-59 0.055781777240653696 1203 60-61 -0.11853866153630577 1203 62-63 0.03307100558297549 1203 64-65 0.05288504199678812 1203 66-67 0.09586172770902834 1203 68-69 0.15867316473657667 1203 70-71 0.2331880680822067 1203 72-73 0.35558173987541153 1203 74-75 -0.08045821808561726 1203 76-77 0.21084708317805934 1203 78-79 0.05504511400602752 1203 80-81 0.05434869865599978 1203 82-83 0.2692503075811743 1203 84-85 0.7029940559578165 1203 86-87 0.21437280915021262 1203 88-89 0.6861082842402197 1203 90-91 0.8375272287800968 1204 1 0.28816225980864374 1204 2 0.16588970669655367 1204 3 -0.08356288518862698 1204 4 -0.02773367184629194 1204 5 -0.005774766382820928 1204 6 -0.051267081809186266 1204 7 -0.26969639034998494 1204 8 -0.1319789711970003 1204 9 -0.16541593837815327 1204 10-11 0.037444939271736644 1204 12-13 -0.1412118566277769 1204 14-15 -0.2507603801319007 1204 16-17 -0.03971850334923488 1204 18-19 0.020549594108878466 1204 20-21 0.011530844227749526 1204 22-23 -0.03695012425837518 1204 24-25 -0.02163348808146992 1204 26-27 -0.0324078385983384 1204 28-29 -0.11964337942742276 1204 30-31 -0.13235100907303377 1204 32-33 -0.20734580779653555 1204 34-35 -0.140317542495616 1204 36-37 -0.14241105781389507 1204 38-39 -0.1267001316004155 1204 40-41 -0.07913351896500842 1204 42-43 -0.26053742589735407 1204 44-45 -0.15262121078907143 1204 46-47 -0.08433197860282604 1204 48-49 -0.08502434269432513 1204 50-51 -0.013583731089219953 1204 52-53 -0.15542808643824202 1204 54-55 0.12611070655317036 1204 56-57 0.053503526544027125 1204 58-59 -0.06965419312013665 1204 60-61 0.23470665995014883 1204 62-63 -0.09369622501121455 1204 64-65 -0.12456600418248343 1204 66-67 -0.13965626786708896 1204 68-69 -0.09785553198732089 1204 70-71 -0.28903045601593647 1204 72-73 -0.33213098147812303 1204 74-75 -0.1470033332041112 1204 76-77 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0.10211791970244377 2401 56-57 0.31835368857849033 2401 58-59 0.18491928263219393 2401 60-61 0.05990461421562543 2401 62-63 -0.057923959165052 2401 64-65 -0.11241362464599547 2401 66-67 0.07192605839393451 2401 68-69 -0.06235610139053449 2401 70-71 0.02166579990169737 2401 72-73 -0.033819703260043354 2401 74-75 0.23533627428921733 2401 76-77 -0.2105255879854795 2401 78-79 0.2366909455965711 2401 80-81 0.10163210936267575 2401 82-83 0.30173145979624394 2401 84-85 0.4765789616181948 2401 86-87 0.5827197069937071 2401 88-89 0.31401526098440513 2401 90-91 -0.18478514444505123 2402 1 0.3976174251109441 2402 2 0.09614076416830386 2402 3 0.3561980525415436 2402 4 0.18220761479694403 2402 5 0.11371848894662406 2402 6 0.0749056070100238 2402 7 0.03947257364336565 2402 8 0.08686401792314058 2402 9 0.13756181788776445 2402 10-11 0.06240548137068913 2402 12-13 0.1159863167344497 2402 14-15 0.1037820134369909 2402 16-17 0.06958724803442351 2402 18-19 0.13217231344219726 2402 20-21 0.051300199643478095 2402 22-23 0.0066261294506730906 2402 24-25 -0.053622797620811014 2402 26-27 0.066845091808446 2402 28-29 0.051063074832974564 2402 30-31 0.09140369956982397 2402 32-33 0.156677593318733 2402 34-35 0.07597089061536622 2402 36-37 0.09234956856934673 2402 38-39 0.07142062116190573 2402 40-41 0.0348308684750549 2402 42-43 0.13376080499149623 2402 44-45 0.21309911728102549 2402 46-47 0.11480038178232377 2402 48-49 0.075844003696659 2402 50-51 0.13647502816598234 2402 52-53 0.09220140790733211 2402 54-55 0.18363808787352554 2402 56-57 0.18856889529755705 2402 58-59 0.20728918016266817 2402 60-61 0.14557816300553839 2402 62-63 -0.004798971034638555 2402 64-65 0.0621046086298378 2402 66-67 0.09863389030026326 2402 68-69 -0.014600466575046767 2402 70-71 0.08603977187051726 2402 72-73 -0.04695762741073395 2402 74-75 0.13240476576849858 2402 76-77 0.2101043037665633 2402 78-79 0.2256305940997052 2402 80-81 0.41378829677366014 2402 82-83 0.38689478132320687 2402 84-85 0.5310400329693152 2402 86-87 0.3647763388864078 2402 88-89 -0.03362759250225622 2402 90-91 0.3708605621134282 2403 1 -0.10880984525075377 2403 2 -0.016635712542601766 2403 3 -0.004405642488379158 2403 4 0.13219460412820183 2403 5 0.030918593031977082 2403 6 0.07964149043443314 2403 7 0.1996599272733377 2403 8 0.32740526174307405 2403 9 0.12944316058877803 2403 10-11 0.18382104213051775 2403 12-13 0.2510370583425754 2403 14-15 0.1989940873375886 2403 16-17 0.046116001612354296 2403 18-19 0.062226958250938935 2403 20-21 0.13560933313294754 2403 22-23 -0.010730102659657348 2403 24-25 0.08941649459880097 2403 26-27 0.070514100395485 2403 28-29 0.013149986100216893 2403 30-31 0.07667562254665938 2403 32-33 0.11450797791864886 2403 34-35 0.14338571275077072 2403 36-37 0.05943829746932039 2403 38-39 0.198186639446007 2403 40-41 0.16423878034813555 2403 42-43 0.2292906927742706 2403 44-45 0.04241874787656741 2403 46-47 0.14049382977486857 2403 48-49 0.08376010604074935 2403 50-51 0.19131436088314757 2403 52-53 0.013839931604657352 2403 54-55 0.09339013453560341 2403 56-57 0.03863500271830844 2403 58-59 0.11728110475631581 2403 60-61 0.04310047262158179 2403 62-63 0.27973042760800126 2403 64-65 0.27965450509256584 2403 66-67 0.2896575193163997 2403 68-69 0.17742692298573104 2403 70-71 0.3602792477249608 2403 72-73 0.25403045907600585 2403 74-75 0.13930127161426498 2403 76-77 0.28831170012669105 2403 78-79 0.035383753747467495 2403 80-81 0.004490397367689525 2403 82-83 0.26735291437168485 2403 84-85 0.09655147892700455 2403 86-87 0.28320771143542345 2403 88-89 0.3428381848725621 2403 90-91 0.8375272287800968 2404 1 -0.4503544117953169 2404 2 -0.39349773940462995 2404 3 -0.5643568024395407 2404 4 -0.4137150417814439 2404 5 -0.32909361698023787 2404 6 -0.11245252665958105 2404 7 -0.11911296649955716 2404 8 0.02288755722536706 2404 9 0.06002924117485975 2404 10-11 0.06520077351024156 2404 12-13 0.025853908159426453 2404 14-15 0.03437259771610712 2404 16-17 0.011012216508568429 2404 18-19 -0.027196118672137004 2404 20-21 0.0487045712281855 2404 22-23 0.03394327951372844 2404 24-25 0.0934610611433726 2404 26-27 -0.04931190693052656 2404 28-29 0.03244180539203256 2404 30-31 -0.13278713691609312 2404 32-33 0.08041932549614472 2404 34-35 -0.07456205632882984 2404 36-37 0.026120475336107063 2404 38-39 0.08902867571255513 2404 40-41 0.044254740171098206 2404 42-43 -0.013928836788004162 2404 44-45 0.17080604323886917 2404 46-47 -0.1227769713473279 2404 48-49 0.0304137356114893 2404 50-51 0.010302437578069146 2404 52-53 -0.10506513187616662 2404 54-55 -0.04280604548976186 2404 56-57 0.0648496873517459 2404 58-59 -0.027098103852623012 2404 60-61 -0.1625660793500785 2404 62-63 0.09880520943666937 2404 64-65 -0.07074205177409709 2404 66-67 0.10367843704371893 2404 68-69 0.06650308213134082 2404 70-71 0.130868009624038 2404 72-73 0.17964189025134658 2404 74-75 0.1491873713238121 2404 76-77 0.289640857596396 2404 78-79 0.25564245173511324 2404 80-81 0.1036604291809411 2404 82-83 0.36590680203040904 2404 84-85 0.07719060928804566 2404 86-87 -0.20108668540327557 2404 88-89 -0.6625287315416983 2404 90-91 0.5708605621134311 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 5.0 31 182.0 32 1199.0 33 2277.0 34 2937.0 35 3816.0 36 5354.0 37 8089.0 38 21790.0 39 131965.0 40 123180.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.155557033146053 12.238438777884902 26.267163137072373 32.33884105189667 2 26.40695807136944 18.409232211667938 28.962706093964552 26.221103622998076 3 26.85552426768088 19.1554933492026 20.973366889301875 33.01561549381464 4 27.684062846998277 20.410978942399115 19.908974248156547 31.99598396244606 5 28.240922358823646 20.50672553308909 20.358451298895588 30.89390080919167 6 28.00554532337746 23.57793041084596 22.863155515070112 25.55336875070646 7 26.666422867477408 23.15006283370014 22.22816944486925 27.9553448539532 8 25.502503374402412 24.225549711762866 22.721862803114423 27.55008411072029 9 25.122176639161687 24.39244133859053 23.50844764190775 26.976934380340033 10-11 25.174538055945266 22.885263668823182 23.286202517337447 28.65399575789411 12-13 25.664241972911693 22.70174937000073 22.975192324314982 28.658816332772595 14-15 26.10042088605491 22.427309055366795 22.804643709648463 28.66762634892983 16-17 26.371702893009836 22.15502968809218 22.381264253941236 29.092003164956747 18-19 26.19251048890603 22.57375479564087 22.47401876367215 28.75971595178095 20-21 26.178381217710463 22.73216885975119 22.37162310418426 28.71782681835409 22-23 26.102581833414227 22.2356496472669 22.46304780015559 29.19872071916328 24-25 26.186692553707854 22.578409143799412 22.50826146798141 28.726636834511325 26-27 26.361396836373064 22.68778632552511 22.545662479969682 28.405154358132144 28-29 26.401623702600453 22.3450268289926 22.53086830189432 28.72248116651263 30-31 26.44976079736428 22.46327183991542 22.66158229468302 28.425385068037272 32-33 26.47669704889858 22.627884741122664 22.759331624863503 28.136086585115255 34-35 26.55210924857417 22.333261450043743 22.735983196842746 28.37864610453934 36-37 26.55540294038561 22.556885487750815 22.828322468586713 28.059389103276867 38-39 26.46632915795483 22.762933527923867 22.94252647578142 27.828210838339878 40-41 26.554848599179437 22.279792746113987 22.85402744553462 28.311331209171954 42-43 26.60447227673326 22.381277029589306 23.075472519183627 27.938778174493805 44-45 26.600858387828087 22.665017095751484 23.051226159227234 27.682898357193196 46-47 26.657824015870634 22.442186364632473 22.87655275025663 28.023436869240264 48-49 26.544444988014288 22.633432806614156 22.978572476884693 27.843549728486867 50-51 26.748842691980425 22.715503894123557 23.080846324587494 27.45480708930853 52-53 26.86861394935584 22.344791203909374 22.878442914260326 27.908151932474457 54-55 26.913821157558758 22.61900866518132 22.957524845838304 27.509645331421616 56-57 26.95459692536034 22.79781973117695 22.917772901459273 27.329810442003442 58-59 27.14384759460662 22.56451117756829 22.595555187626548 27.69608604019854 60-61 26.83849477383144 22.80543865086804 22.804691788623046 27.551374786677474 62-63 27.06942128594087 22.872422628004394 22.730895929073977 27.327260156980763 64-65 27.073071977432804 22.50935479708655 22.61969913370797 27.797874091772673 66-67 27.107184093622184 22.613456232020194 22.666294839429344 27.613064834928274 68-69 27.354905760465947 22.648883307531584 22.462677680224314 27.533533251778152 70-71 27.514185681516356 22.532898708197514 22.235301219364963 27.717614390921163 72-73 27.201360101872176 22.822346853071064 22.495868216424178 27.480424828632582 74-75 27.46446971383819 22.817699495550556 22.286376793789977 27.431453996821276 76-77 27.62116016826328 22.597122556470715 21.939623175085725 27.842094100180283 78-79 27.61962634148351 22.618287432797082 22.01989824293983 27.742187982779576 80-81 27.70510712445088 22.69492232556998 21.563550690848405 28.036419859130735 82-83 28.579425113464445 22.400907715582452 20.878971255673225 28.140695915279878 84-85 28.51965865253478 22.370728285859016 20.19834002260063 28.911273039005575 86-87 29.337308578398158 21.51511044857027 19.899715408591952 29.247865564439625 88-89 28.43374535396318 20.866107701616386 19.980119284294233 30.7200276601262 90-91 23.86061720825669 21.827099938687923 21.2650725526262 33.047210300429185 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 2.0 10 3.5 11 5.5 12 7.5 13 8.5 14 14.5 15 27.0 16 34.5 17 40.5 18 54.5 19 77.5 20 112.0 21 154.5 22 192.5 23 249.5 24 341.5 25 440.0 26 550.5 27 688.5 28 865.5 29 1090.5 30 1350.5 31 1659.0 32 2045.5 33 2444.5 34 2899.5 35 3430.5 36 3990.5 37 4642.0 38 5378.0 39 6210.0 40 7103.0 41 8122.5 42 9249.5 43 10361.0 44 11494.0 45 12677.0 46 13978.5 47 15122.5 48 15942.5 49 16758.5 50 17538.5 51 18343.0 52 19061.0 53 19533.0 54 19909.0 55 20073.5 56 20137.0 57 20156.5 58 20142.5 59 20076.0 60 19801.5 61 19458.0 62 18988.5 63 18303.5 64 17396.0 65 16276.0 66 14798.5 67 13386.0 68 12057.0 69 10567.0 70 9166.5 71 7701.0 72 6327.5 73 5051.0 74 3922.0 75 3144.0 76 2495.0 77 1844.0 78 1326.5 79 933.0 80 638.5 81 446.0 82 309.5 83 197.5 84 145.5 85 117.5 86 78.5 87 44.5 88 28.0 89 19.5 90 13.0 91 11.0 92 4.5 93 2.0 94 3.5 95 3.0 96 2.0 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0013298137595829705 2 0.0069815222378105946 3 9.97360319687228E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.00151283641699823 84-85 0.00194829232177996 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 4113.0 32-33 9081.0 34-35 9800.0 36-37 10525.0 38-39 11215.0 40-41 11791.0 42-43 12058.0 44-45 12356.0 46-47 12331.0 48-49 12362.0 50-51 12083.0 52-53 12042.0 54-55 11816.0 56-57 11462.0 58-59 11086.0 60-61 11053.0 62-63 10641.0 64-65 10315.0 66-67 9841.0 68-69 9682.0 70-71 9085.0 72-73 8751.0 74-75 8674.0 76-77 8039.0 78-79 7936.0 80-81 7705.0 82-83 7442.0 84-85 7300.0 86-87 6949.0 88-89 6736.0 90-91 6524.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.69858517117582 #Duplication Level Percentage of deduplicated Percentage of total 1 99.71679418726825 99.41623298275974 2 0.2680551740936376 0.5344944320989778 3 0.012333979475398715 0.036890409096827205 4 0.0016636852210952088 0.006634682508535485 5 0.0011529739416186 0.005747493535930415 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0019947206393744555 0.0 0.0 0.0 0.0 2 0.0019947206393744555 0.0 0.0 0.0 0.0 3 0.0019947206393744555 0.0 0.0 0.0 0.0 4 0.0019947206393744555 0.0 0.0 0.0 0.0 5 0.002327174079270198 0.0 0.0 0.0 3.324534398957426E-4 6 0.002327174079270198 0.0 0.0 0.0 3.324534398957426E-4 7 0.002327174079270198 0.0 0.0 0.0 3.324534398957426E-4 8 0.0069815222378105946 0.0 0.0 0.0 3.324534398957426E-4 9 0.0069815222378105946 0.0 0.0 0.0 3.324534398957426E-4 10-11 0.0069815222378105946 0.0 0.0 0.0 6.649068797914852E-4 12-13 0.0069815222378105946 0.0 0.0 0.0 6.649068797914852E-4 14-15 0.0069815222378105946 0.0 0.0 0.0 6.649068797914852E-4 16-17 0.0069815222378105946 0.0 0.0 0.0 6.649068797914852E-4 18-19 0.0069815222378105946 0.0 0.0 0.0 6.649068797914852E-4 20-21 0.0069815222378105946 0.0 0.0 0.0 6.649068797914852E-4 22-23 0.0069815222378105946 0.0 0.0 0.0 6.649068797914852E-4 24-25 0.0069815222378105946 0.0 0.0 0.0 6.649068797914852E-4 26-27 0.007147748957758466 0.0 0.0 0.0 6.649068797914852E-4 28-29 0.007313975677706338 0.0 0.0 0.0 6.649068797914852E-4 30-31 0.007313975677706338 0.0 0.0 0.0 6.649068797914852E-4 32-33 0.007313975677706338 0.0 0.0 0.0 6.649068797914852E-4 34-35 0.007313975677706338 0.0 0.0 0.0 6.649068797914852E-4 36-37 0.007313975677706338 0.0 0.0 0.0 6.649068797914852E-4 38-39 0.007480202397654208 0.0 0.0 0.0 6.649068797914852E-4 40-41 0.00764642911760208 0.0 0.0 0.0 6.649068797914852E-4 42-43 0.00764642911760208 0.0 0.0 0.0 6.649068797914852E-4 44-45 0.00764642911760208 0.0 0.0 0.0 6.649068797914852E-4 46-47 0.007812655837549951 0.0 0.0 0.0 6.649068797914852E-4 48-49 0.007978882557497822 0.0 0.0 0.0 6.649068797914852E-4 50-51 0.008145109277445692 0.0 0.0 0.0 6.649068797914852E-4 52-53 0.008311335997393565 0.0 0.0 0.0 6.649068797914852E-4 54-55 0.008311335997393565 0.0 0.0 0.0 6.649068797914852E-4 56-57 0.008311335997393565 0.0 0.0 0.0 6.649068797914852E-4 58-59 0.008477562717341437 0.0 0.0 0.0 6.649068797914852E-4 60-61 0.008643789437289308 0.0 0.0 0.0 6.649068797914852E-4 62-63 0.008643789437289308 0.0 0.0 0.0 6.649068797914852E-4 64-65 0.008643789437289308 0.0 0.0 0.0 6.649068797914852E-4 66-67 0.008643789437289308 0.0 0.0 0.0 6.649068797914852E-4 68-69 0.008643789437289308 0.0 0.0 0.0 6.649068797914852E-4 70-71 0.008643789437289308 0.0 0.0 0.0 6.649068797914852E-4 72-73 0.008976242877185051 0.0 0.0 0.0 6.649068797914852E-4 74-75 0.008976242877185051 0.0 0.0 0.0 6.649068797914852E-4 76-77 0.008976242877185051 0.0 0.0 0.0 6.649068797914852E-4 78-79 0.008976242877185051 0.0 0.0 0.0 6.649068797914852E-4 80 0.008976242877185051 0.0 0.0 0.0 6.649068797914852E-4 >>END_MODULE