##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-8A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 423319 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.23613634162417 40.0 40.0 40.0 40.0 40.0 2 39.13713298954217 40.0 40.0 40.0 40.0 40.0 3 39.11184236946605 40.0 40.0 40.0 40.0 40.0 4 39.07528837590564 40.0 40.0 40.0 40.0 40.0 5 39.19665311502673 40.0 40.0 40.0 40.0 40.0 6 39.269742203869896 40.0 40.0 40.0 40.0 40.0 7 39.306064693528995 40.0 40.0 40.0 40.0 40.0 8 39.307604903158136 40.0 40.0 40.0 40.0 40.0 9 39.350967001244925 40.0 40.0 40.0 40.0 40.0 10-11 39.34468332392357 40.0 40.0 40.0 40.0 40.0 12-13 39.34478253988127 40.0 40.0 40.0 40.0 40.0 14-15 39.35853103687762 40.0 40.0 40.0 40.0 40.0 16-17 39.48175253177864 40.0 40.0 40.0 40.0 40.0 18-19 39.492689909973336 40.0 40.0 40.0 40.0 40.0 20-21 39.503991080012945 40.0 40.0 40.0 40.0 40.0 22-23 39.501161062933626 40.0 40.0 40.0 40.0 40.0 24-25 39.501458710806745 40.0 40.0 40.0 40.0 40.0 26-27 39.487955891419944 40.0 40.0 40.0 40.0 40.0 28-29 39.49204736853295 40.0 40.0 40.0 40.0 40.0 30-31 39.48033204421438 40.0 40.0 40.0 40.0 40.0 32-33 39.48568914855789 40.0 40.0 40.0 40.0 40.0 34-35 39.47292595453816 40.0 40.0 40.0 40.0 40.0 36-37 39.47063278054584 40.0 40.0 40.0 40.0 40.0 38-39 39.457168024546505 40.0 40.0 40.0 40.0 40.0 40-41 39.44374129323337 40.0 40.0 40.0 40.0 40.0 42-43 39.44107714443764 40.0 40.0 40.0 40.0 40.0 44-45 39.42515133777991 40.0 40.0 40.0 40.0 40.0 46-47 39.415161744845776 40.0 40.0 40.0 40.0 40.0 48-49 39.41352621078133 40.0 40.0 40.0 40.0 40.0 50-51 39.412318803843505 40.0 40.0 40.0 40.0 40.0 52-53 39.39683935446949 40.0 40.0 40.0 40.0 40.0 54-55 39.3756987966522 40.0 40.0 40.0 40.0 40.0 56-57 39.379125566877825 40.0 40.0 40.0 40.0 40.0 58-59 39.369250497616804 40.0 40.0 40.0 40.0 40.0 60-61 39.34398270290727 40.0 40.0 40.0 40.0 40.0 62-63 39.359518687441835 40.0 40.0 40.0 40.0 40.0 64-65 39.35429412887618 40.0 40.0 40.0 40.0 40.0 66-67 39.368470731487776 40.0 40.0 40.0 40.0 40.0 68-69 39.3589150847215 40.0 40.0 40.0 40.0 40.0 70-71 39.34124105026895 40.0 40.0 40.0 40.0 40.0 72-73 39.33733805365385 40.0 40.0 40.0 40.0 40.0 74-75 39.350529235043005 40.0 40.0 40.0 40.0 40.0 76-77 39.341193190527314 40.0 40.0 40.0 40.0 40.0 78-79 39.34655238375069 40.0 40.0 40.0 40.0 40.0 80-81 39.34010472267187 40.0 40.0 40.0 40.0 40.0 82-83 39.341143358225665 40.0 40.0 40.0 40.0 40.0 84-85 39.31646849138394 40.0 40.0 40.0 40.0 40.0 86-87 39.36002317310732 40.0 40.0 40.0 40.0 40.0 88-89 39.38708663577849 40.0 40.0 40.0 40.0 40.0 90-91 39.43352789273588 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26324934735842476 1101 2 0.19026114198163668 1101 3 0.23791724445351292 1101 4 0.22839938495434353 1101 5 0.13027490915385442 1101 6 -0.015557073201087235 1101 7 0.17853736905377104 1101 8 0.011599412071554127 1101 9 0.1029843457140629 1101 10-11 0.011512875377007958 1101 12-13 0.05155371340417503 1101 14-15 0.09371882600093073 1101 16-17 -0.04095134903052866 1101 18-19 0.040694699416292224 1101 20-21 -0.05424634675877371 1101 22-23 0.052002675300904855 1101 24-25 0.03766735452891368 1101 26-27 0.12840655206593254 1101 28-29 -0.03726665564841625 1101 30-31 -0.04514202816333324 1101 32-33 -0.12172768392807143 1101 34-35 -0.00827340441916391 1101 36-37 0.08687268227457423 1101 38-39 -0.03126624254507959 1101 40-41 0.06492344643451986 1101 42-43 0.03634059885595775 1101 44-45 0.14634813007941005 1101 46-47 0.040514706345966545 1101 48-49 0.04445325585139415 1101 50-51 -0.24997254001845448 1101 52-53 -0.03649797140581512 1101 54-55 -0.04805119449927986 1101 56-57 -0.06372346779382099 1101 58-59 -0.16695462040419784 1101 60-61 -0.3319490682312818 1101 62-63 -0.09483514074521082 1101 64-65 -0.07798391433769325 1101 66-67 -0.20483898222124708 1101 68-69 0.0376459371077118 1101 70-71 -0.22985437138621023 1101 72-73 0.030684974645133423 1101 74-75 -0.06618624633203041 1101 76-77 0.031382103190161104 1101 78-79 -0.12404497795863989 1101 80-81 -0.03844649132192046 1101 82-83 -0.08406285928913348 1101 84-85 -0.20429760837619426 1101 86-87 0.004060504454550085 1101 88-89 0.5871202059442382 1101 90-91 0.5936554545765844 1102 1 0.34682427569315166 1102 2 0.2641424520839024 1102 3 0.26588804974759483 1102 4 -0.14260921487894507 1102 5 0.10719564024133632 1102 6 0.10950849290377818 1102 7 0.19639574626726386 1102 8 0.11921321246597216 1102 9 0.020068128014258946 1102 10-11 -0.19286226439569987 1102 12-13 -0.02982551281412782 1102 14-15 0.03463817460930585 1102 16-17 -0.050087909503822914 1102 18-19 -0.03143411510787075 1102 20-21 -0.09726584378033465 1102 22-23 0.028939670848515675 1102 24-25 -0.04663540839624858 1102 26-27 -0.01258192049795781 1102 28-29 -0.09634730704004113 1102 30-31 -0.03249641041880835 1102 32-33 -0.06147826451486793 1102 34-35 -0.11638498706263789 1102 36-37 -0.09424388397182781 1102 38-39 -0.06844527538881096 1102 40-41 -0.025106867208101846 1102 42-43 -0.10956540739449139 1102 44-45 -0.05911053548040712 1102 46-47 0.11389925044230864 1102 48-49 0.17870955433888014 1102 50-51 0.1813525760795187 1102 52-53 -0.04812257250068086 1102 54-55 -0.3222382813764568 1102 56-57 0.009755824766983778 1102 58-59 0.014591480237292842 1102 60-61 -0.15864186115816636 1102 62-63 0.22186706765618425 1102 64-65 0.1552015477257953 1102 66-67 -0.05030635564031627 1102 68-69 -0.1440474267366838 1102 70-71 -0.18278792737028482 1102 72-73 -0.07157414589709532 1102 74-75 0.3146789653818942 1102 76-77 0.22556554621089475 1102 78-79 0.19164786875546724 1102 80-81 -0.40350276197713697 1102 82-83 0.16181410898205684 1102 84-85 -0.13833586353685234 1102 86-87 -0.6327108436510898 1102 88-89 0.3112581369787222 1102 90-91 -0.4063445454234156 1103 1 0.34310150341592305 1103 2 0.0985978976284585 1103 3 0.23198705964858135 1103 4 0.10665811185373286 1103 5 -0.08389346866955094 1103 6 -0.01655091303681644 1103 7 0.05156406309894379 1103 8 0.03343103424814586 1103 9 0.02561268246970627 1103 10-11 -0.0034563238016431796 1103 12-13 -0.07869679994283985 1103 14-15 0.16840055084693262 1103 16-17 0.06405070435755533 1103 18-19 -0.03691926362272113 1103 20-21 0.05859554235827602 1103 22-23 -0.003931616280198114 1103 24-25 0.03583983912850641 1103 26-27 0.04422996069015994 1103 28-29 -0.058584930802418 1103 30-31 -0.030139974775245548 1103 32-33 0.05998915903607127 1103 34-35 0.023916411404421467 1103 36-37 -0.05648337670236714 1103 38-39 0.022976000105309424 1103 40-41 -0.0018813388471627945 1103 42-43 -0.05235375580038948 1103 44-45 -0.06357775284623557 1103 46-47 0.021285610620601858 1103 48-49 0.008860610490074805 1103 50-51 -0.002556520030857712 1103 52-53 -0.05638927199479582 1103 54-55 -0.034883171643272703 1103 56-57 -0.047323261432559605 1103 58-59 0.044774404054123806 1103 60-61 0.10915599121720021 1103 62-63 -0.11127094624429645 1103 64-65 -0.03338207107889701 1103 66-67 0.17123458452341112 1103 68-69 -0.16369133203740205 1103 70-71 0.03912772720219948 1103 72-73 -0.07536653725764353 1103 74-75 0.02668319644849504 1103 76-77 -0.2071631279781272 1103 78-79 -0.21142623834548857 1103 80-81 -0.004059662527495789 1103 82-83 0.1201736579298398 1103 84-85 0.10930007142091114 1103 86-87 0.3443935334532924 1103 88-89 -0.2644204103022574 1103 90-91 0.5936554545765844 1104 1 0.26785867358800175 1104 2 0.04039522076421065 1104 3 0.08352052045164271 1104 4 -0.052133475144358954 1104 5 -0.06725866943437353 1104 6 0.17625214623660668 1104 7 -0.16173612810564464 1104 8 0.04351134017549185 1104 9 0.061406181513682156 1104 10-11 0.04704080812952327 1104 12-13 -0.0405935494648304 1104 14-15 0.023601315665281675 1104 16-17 0.08098378275143858 1104 18-19 -0.029764387905700573 1104 20-21 -0.009217077144597852 1104 22-23 -0.04224901589779506 1104 24-25 -0.02570891804166564 1104 26-27 -0.03487902592551961 1104 28-29 0.07617223936966155 1104 30-31 -0.02454532850374136 1104 32-33 0.011310649698820896 1104 34-35 -0.024418169476525975 1104 36-37 -0.1099605292306336 1104 38-39 -0.10537125306074557 1104 40-41 -0.03291968671986467 1104 42-43 0.043663855760861736 1104 44-45 -0.05829327675799334 1104 46-47 -0.233571016878507 1104 48-49 -0.01456865197387458 1104 50-51 -0.030087314713718172 1104 52-53 -0.02529467694944998 1104 54-55 -0.031162986516989122 1104 56-57 0.033788733916722435 1104 58-59 -0.09509278655092146 1104 60-61 0.06261564581466672 1104 62-63 -0.18723801693603548 1104 64-65 -0.15339575709458586 1104 66-67 -0.31159712713441934 1104 68-69 -0.3441075427351592 1104 70-71 -0.3118347675865323 1104 72-73 -0.30676863629604867 1104 74-75 -0.27452648285670733 1104 76-77 -0.48572983353892596 1104 78-79 -0.004013581955405243 1104 80-81 -0.14671637624879708 1104 82-83 -0.09157237381619154 1104 84-85 0.23667091336746182 1104 86-87 0.2651727542325091 1104 88-89 0.29808794787972204 1104 90-91 0.3936554545765816 1201 1 0.3377785462174785 1201 2 0.3866912828424631 1201 3 0.0894500946680381 1201 4 0.19914060212610707 1201 5 0.1546279321086601 1201 6 0.1784841064184377 1201 7 -0.11031920538354711 1201 8 0.33556332996798943 1201 9 0.06249984200277936 1201 10-11 0.14125184740458963 1201 12-13 0.005552227294515433 1201 14-15 0.02026825512708541 1201 16-17 -0.006750852062673118 1201 18-19 -0.08019552821416198 1201 20-21 0.00492239060730526 1201 22-23 -0.0500794762023844 1201 24-25 0.048641395548735034 1201 26-27 -0.021007393395436225 1201 28-29 0.004068765695635079 1201 30-31 0.12913628981619496 1201 32-33 0.05713538485483127 1201 34-35 0.008806502897968471 1201 36-37 0.06870948053784787 1201 38-39 0.11562480669443431 1201 40-41 -0.07899037844771328 1201 42-43 -0.020019635609394015 1201 44-45 0.0397094517374299 1201 46-47 0.08070982124171877 1201 48-49 0.10364363393122744 1201 50-51 0.17985524876358738 1201 52-53 0.06810234176877117 1201 54-55 0.011253120435789299 1201 56-57 0.1460900538766623 1201 58-59 0.023998170152282228 1201 60-61 0.033805738762438864 1201 62-63 0.004138630985536906 1201 64-65 -0.1575592788154836 1201 66-67 0.2312249495479719 1201 68-69 -0.22360728988345357 1201 70-71 -0.16939838579575905 1201 72-73 -0.24128080467358615 1201 74-75 -0.30902216649099756 1201 76-77 0.0851812379679373 1201 78-79 -0.004642706341080327 1201 80-81 -0.15477771042535693 1201 82-83 -0.07384700409841116 1201 84-85 -0.6279019605602798 1201 86-87 0.14106412773106314 1201 88-89 -0.6142086977102466 1201 90-91 -0.9431866506865774 1202 1 0.21442860621965565 1202 2 0.25411191632004915 1202 3 0.11206182600372472 1202 4 0.10075156979765865 1202 5 0.03105980235848449 1202 6 0.1397481523837456 1202 7 0.1375079883325867 1202 8 0.12920673518272707 1202 9 0.20953791611457007 1202 10-11 0.040375451899294035 1202 12-13 -0.04736969713910355 1202 14-15 0.08643793402449518 1202 16-17 -0.1648838750816921 1202 18-19 -0.026844497267575207 1202 20-21 0.06238993488163658 1202 22-23 -0.033464326560576296 1202 24-25 0.029204325109809304 1202 26-27 -0.028349478562176955 1202 28-29 -0.02398679996129971 1202 30-31 0.11316843643970032 1202 32-33 -0.037833177778288984 1202 34-35 0.0664545396307048 1202 36-37 0.08584272077972344 1202 38-39 0.03065901042941732 1202 40-41 -0.0684253644813424 1202 42-43 -0.06207781756607034 1202 44-45 -0.06163240704155726 1202 46-47 -0.023933113718051402 1202 48-49 -0.056473706203689744 1202 50-51 -0.001770372604504189 1202 52-53 -0.1902965641063119 1202 54-55 -0.09550318622163445 1202 56-57 -0.23777178893990936 1202 58-59 -0.10251877634737383 1202 60-61 -0.15698855905280595 1202 62-63 -0.17567556026324382 1202 64-65 0.09367640934662091 1202 66-67 -0.03533881384677784 1202 68-69 -0.2839393868652209 1202 70-71 -0.30338224533301883 1202 72-73 -0.2968319608345027 1202 74-75 -0.22138685341482045 1202 76-77 -0.5127261220255974 1202 78-79 -1.036573550113438 1202 80-81 -0.4958517326613432 1202 82-83 -0.21684316037730156 1202 84-85 -0.10464203635334712 1202 86-87 -0.13859014953039406 1202 88-89 -1.1989760507402494 1202 90-91 -0.22987395718812564 1203 1 0.2312057864699213 1203 2 0.11096288831747358 1203 3 -0.1533239273160376 1203 4 0.06287412675131065 1203 5 0.06750317937513728 1203 6 -0.01825668584873341 1203 7 -0.09883444714314038 1203 8 -0.10703823949486946 1203 9 -0.09416385384314196 1203 10-11 0.07622710264156751 1203 12-13 -0.057780598825523555 1203 14-15 -0.14048213071732363 1203 16-17 -0.08592322487894677 1203 18-19 0.06084237511098678 1203 20-21 -0.014130714624968732 1203 22-23 -0.003484688905899702 1203 24-25 0.08312103093484069 1203 26-27 -0.07291342850909643 1203 28-29 -0.02007096432197386 1203 30-31 0.1259009935673987 1203 32-33 0.02037350000538396 1203 34-35 0.04438377343932132 1203 36-37 0.04426076794914735 1203 38-39 0.20610370573300685 1203 40-41 -0.023050305059598486 1203 42-43 0.06729386456679975 1203 44-45 0.005666871780555027 1203 46-47 0.16657373720168067 1203 48-49 0.08901214166927218 1203 50-51 0.030585040320396217 1203 52-53 0.1197018340910816 1203 54-55 0.09866977559700274 1203 56-57 0.1289922900320306 1203 58-59 0.14873956110294273 1203 60-61 0.1016670902611807 1203 62-63 0.025705952673853005 1203 64-65 -0.11169405926523268 1203 66-67 -0.1378694634497606 1203 68-69 -0.12779230233712724 1203 70-71 0.0608264134354215 1203 72-73 0.3050119219470062 1203 74-75 -0.24388657349025067 1203 76-77 0.3345777199132769 1203 78-79 0.21017680191854282 1203 80-81 0.22087181544003442 1203 82-83 -0.1800883682727772 1203 84-85 -0.06916465108368186 1203 86-87 0.18739310463681846 1203 88-89 0.2537868726109096 1203 90-91 -1.8484498085813144 1204 1 0.2934928469686682 1204 2 0.19100547201980334 1204 3 0.18511239779105182 1204 4 -0.03237164468369258 1204 5 0.23540319499140594 1204 6 0.22131022312188264 1204 7 0.18542519925764367 1204 8 0.03737693145283316 1204 9 0.041627109881787305 1204 10-11 -0.14139140044726872 1204 12-13 -0.008161182269262213 1204 14-15 -0.06297005330095118 1204 16-17 -0.0861314417199921 1204 18-19 -0.032964349285478534 1204 20-21 0.029670384558457386 1204 22-23 -0.010532157308155377 1204 24-25 -0.11273545392830187 1204 26-27 -0.060045963565926286 1204 28-29 -0.19395553495029816 1204 30-31 -0.02179191345874898 1204 32-33 -0.004022076792502105 1204 34-35 0.005110410534975074 1204 36-37 -0.034098977786122475 1204 38-39 -0.18116732389095347 1204 40-41 -0.18680260836584495 1204 42-43 0.003874183116444385 1204 44-45 -0.06525345833205165 1204 46-47 -0.2629801605523525 1204 48-49 -0.3495842129069331 1204 50-51 -0.1135930634848279 1204 52-53 -0.0016719963624538536 1204 54-55 -0.06844381163145385 1204 56-57 -0.06363277297804615 1204 58-59 -0.20938891173311447 1204 60-61 -0.14127593818987094 1204 62-63 -0.25121358882575606 1204 64-65 -0.2735211918635656 1204 66-67 -0.31805590060558586 1204 68-69 -0.11057454658553212 1204 70-71 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0.1185136163835665 2401 46-47 0.01362298034731424 2401 48-49 0.11234679818628024 2401 50-51 0.034996275060386495 2401 52-53 0.11351924866396956 2401 54-55 0.16078109868143997 2401 56-57 0.1848736543588103 2401 58-59 0.28043660825814243 2401 60-61 0.08128894424032751 2401 62-63 0.11472209524443855 2401 64-65 0.2524007936405468 2401 66-67 -0.011384030456781602 2401 68-69 0.2812699727406951 2401 70-71 0.1603475086222943 2401 72-73 0.08678557339279536 2401 74-75 0.21760418835602735 2401 76-77 -0.12262219119086382 2401 78-79 0.005954705535707205 2401 80-81 -0.08861160728413608 2401 82-83 0.2632268165534697 2401 84-85 0.3027317304487056 2401 86-87 0.25725710896746534 2401 88-89 0.11336134069600945 2401 90-91 -1.073011212090087 2402 1 0.4462691334737272 2402 2 0.282181712657362 2402 3 0.277952377567658 2402 4 0.22776793844332843 2402 5 0.12940132901830737 2402 6 0.13972654361057835 2402 7 0.09921724228969708 2402 8 0.21419404002849518 2402 9 0.052895919463928465 2402 10-11 0.15324887851049596 2402 12-13 0.08851707288953037 2402 14-15 0.11504794969085452 2402 16-17 0.10483683152518353 2402 18-19 0.08471079418075078 2402 20-21 -0.03138133625444084 2402 22-23 -0.011099246338005742 2402 24-25 0.056128856469548793 2402 26-27 0.06461146358033432 2402 28-29 0.07770408653862404 2402 30-31 0.034750198635158824 2402 32-33 0.12787191008893473 2402 34-35 -0.013898543675892938 2402 36-37 -0.0021880148937682975 2402 38-39 0.06737605573053429 2402 40-41 0.010938213856888979 2402 42-43 0.024123062540823526 2402 44-45 0.051995785745525325 2402 46-47 0.026582564948988363 2402 48-49 -0.01593879610946658 2402 50-51 -0.05590185471353237 2402 52-53 0.019565376020700853 2402 54-55 0.09171836863309579 2402 56-57 0.18154630562573004 2402 58-59 0.21896385042334998 2402 60-61 0.15553101844438544 2402 62-63 0.1752012234936089 2402 64-65 0.2061958059954634 2402 66-67 0.11230571039455128 2402 68-69 0.10669797547528503 2402 70-71 0.08923039326369064 2402 72-73 0.13895007555729677 2402 74-75 0.2675314675844689 2402 76-77 -0.0056650549452896826 2402 78-79 0.21018415099637622 2402 80-81 0.047768028470699164 2402 82-83 0.06700417508294265 2402 84-85 0.308295483011662 2402 86-87 0.1032679923277513 2402 88-89 0.3014059202299535 2402 90-91 -0.16824930732817478 2403 1 -0.5163245055526886 2403 2 -0.3248998602190767 2403 3 -0.26284702106890734 2403 4 -0.04243605854985333 2403 5 0.18606168828112146 2403 6 -0.037914141736365536 2403 7 0.022613390453201987 2403 8 -0.013331297590418956 2403 9 0.055747211617685366 2403 10-11 0.13963784660194278 2403 12-13 0.039590195572856146 2403 14-15 0.17073238941195257 2403 16-17 -0.006550192503432584 2403 18-19 0.06528701440419127 2403 20-21 0.04350137195469017 2403 22-23 0.07548542407854342 2403 24-25 -0.009855228136068206 2403 26-27 0.038741171452493006 2403 28-29 0.02965722166394613 2403 30-31 0.12042504764627893 2403 32-33 0.0707598021229856 2403 34-35 -0.02280799092284269 2403 36-37 0.09439411002071552 2403 38-39 0.053582859789962356 2403 40-41 -0.02084664141683845 2403 42-43 0.0737968920771479 2403 44-45 0.10380145236418059 2403 46-47 0.16704937621491212 2403 48-49 0.03164346939628615 2403 50-51 0.01855569455407391 2403 52-53 0.20242442399868565 2403 54-55 0.16608820601267382 2403 56-57 0.12166073089438356 2403 58-59 -0.157905258625604 2403 60-61 0.08705460250038755 2403 62-63 0.11539956064266477 2403 64-65 0.10510000385892226 2403 66-67 0.21228049710826014 2403 68-69 0.20947564581452838 2403 70-71 0.2291217352941004 2403 72-73 0.222436830724142 2403 74-75 0.2228782380427745 2403 76-77 0.3559870956396125 2403 78-79 0.1731022122900896 2403 80-81 -0.11754358185536518 2403 82-83 -0.10765004001748935 2403 84-85 0.2650435527457873 2403 86-87 0.06561681857250079 2403 88-89 0.5871202059442382 2403 90-91 0.14921101013214155 2404 1 -0.4507522648630413 2404 2 -0.3737530585020252 2404 3 -0.4421006793840405 2404 4 -0.4731664100810278 2404 5 -0.21651439105425396 2404 6 -0.2654283033630236 2404 7 -0.20682513825088478 2404 8 -0.10571857205106738 2404 9 0.04918973533809634 2404 10-11 0.04112185392614265 2404 12-13 0.04875426867357646 2404 14-15 -0.0030167719877240984 2404 16-17 0.008491649239445564 2404 18-19 -0.007103740563096039 2404 20-21 0.021657409624864954 2404 22-23 0.022255110378964105 2404 24-25 0.05927066027586392 2404 26-27 0.04053817216373545 2404 28-29 -0.06876040886766077 2404 30-31 -0.014821639567152545 2404 32-33 0.041189831008033195 2404 34-35 0.06422658957266236 2404 36-37 0.015060007615616655 2404 38-39 0.0030272028430218256 2404 40-41 0.07907541130292373 2404 42-43 0.031204114823545126 2404 44-45 -0.058277959643440624 2404 46-47 0.009547929345920636 2404 48-49 0.08033104511306988 2404 50-51 0.04217381442357038 2404 52-53 0.002369277419219884 2404 54-55 0.06420478689242515 2404 56-57 0.034030383976670464 2404 58-59 0.05840059423934463 2404 60-61 0.15762426735055612 2404 62-63 0.13427705669036527 2404 64-65 0.36344756966860814 2404 66-67 0.2474671795185941 2404 68-69 0.24739305252631283 2404 70-71 0.24752254230189408 2404 72-73 0.20484148361864385 2404 74-75 0.08567264499173888 2404 76-77 0.06632280231625032 2404 78-79 0.34136762690896205 2404 80-81 0.2969872857006379 2404 82-83 -0.008348179061030692 2404 84-85 0.06608284823221311 2404 86-87 0.1545833436431039 2404 88-89 -0.6628797940557618 2404 90-91 0.5936554545765844 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 14.0 31 250.0 32 1757.0 33 3158.0 34 4074.0 35 5222.0 36 7433.0 37 11141.0 38 29152.0 39 178653.0 40 182465.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.859391757496727 12.323595972767517 26.661516873839524 32.155495395896224 2 26.48189315674958 18.48270583260295 29.25538574935092 25.780015261296548 3 26.799837473660336 19.354808228368405 21.451586994113146 32.39376730385811 4 27.544712143796996 20.927716450242016 20.318010767293696 31.209560638667295 5 28.0197675984305 20.828972949477816 20.673770844209685 30.477488607881998 6 27.69424476576766 23.681904190456844 23.279607104807486 25.344243938968013 7 26.19443020511718 23.417092074771034 22.90164155164309 27.4868361684687 8 25.305266241297936 24.429094843368713 23.339136679430876 26.92650223590248 9 24.87556665304416 24.59303740205377 24.049003233967763 26.482392710934306 10-11 24.978798494752187 23.087199015399733 23.90017929740928 28.0338231924388 12-13 25.335503485551087 22.8404583777246 23.725370229070748 28.098667907653567 14-15 25.694216418351175 22.631986752307363 23.54158447884456 28.132212350496903 16-17 25.823197163368523 22.3483944731987 23.236967865841127 28.591440497591652 18-19 25.88012822481391 22.66765725138725 23.120979686713802 28.331234837085034 20-21 26.002967029592337 22.79864593840579 23.040307663960277 28.158079368041594 22-23 25.909656783654878 22.361977610265544 23.02637018418734 28.701995421892235 24-25 25.930208660608194 22.680177360335822 23.134090366839192 28.25552361221679 26-27 26.150964166503275 22.79226776969614 23.11082186247251 27.945946201328077 28-29 26.228092762195885 22.444775689255618 23.032275895955532 28.29485565259296 30-31 26.107888542024703 22.636490663605127 23.170488275321183 28.085132519048983 32-33 26.177132822423626 22.700876104194638 23.20860320057819 27.913387872803547 34-35 26.31541422179638 22.32561106642834 23.287006403048046 28.071968308727236 36-37 26.17205120172215 22.592168429591133 23.3722576309259 27.863522737760814 38-39 26.253352169657067 22.75163981767451 23.359572223834096 27.63543578883433 40-41 26.266406380977546 22.446514984897178 23.402837568658875 27.884241065466398 42-43 26.222170377781467 22.668533066533865 23.516189672886505 27.593106882798164 44-45 26.349331670074765 22.679793776991765 23.558057461372663 27.412817091560804 46-47 26.393577686681134 22.44209334726576 23.410999919620608 27.753329046432494 48-49 26.39060425410574 22.723376195186283 23.42574028033969 27.460279270368293 50-51 26.49015446403929 22.808142910167216 23.372702787585773 27.328999838207725 52-53 26.606822848418794 22.465312220630196 23.40358654697903 27.52427838397198 54-55 26.52922125750017 22.744452237463804 23.402730292883163 27.32359621215286 56-57 26.641813195293672 22.76110985788405 23.355218635354458 27.24185831146782 58-59 26.772795289887735 22.41604446378947 23.325975929183482 27.485184317139318 60-61 26.719516608178278 22.84553734981668 23.15102347670703 27.283922565298006 62-63 26.863975128565897 22.864761577075026 23.08379977512488 27.187463519234196 64-65 27.006622157821326 22.478772530910174 22.911447259726717 27.603158051541786 66-67 26.871037445090646 22.6939943302463 22.86101613670323 27.573952087959825 68-69 26.91683798356192 22.92616207064789 23.01122977035891 27.145770175431284 70-71 27.07136267636963 22.527547145071484 22.81273560828498 27.588354570273903 72-73 27.335847853803656 22.714619634509138 22.516468338291542 27.433064173395667 74-75 27.244932073063243 22.929824028204116 22.490958271282256 27.334285627450384 76-77 27.547566324875532 22.76625153382886 22.233818987038266 27.45236315425734 78-79 27.466150870406192 22.817314746881877 22.201193890482223 27.515340492229708 80-81 27.90301572988679 22.95361186253882 21.72427612463681 27.419096282937584 82-83 28.02916257138236 22.256366871119848 21.264789678639413 28.44968087885838 84-85 28.566806858621806 21.98479456486574 20.875121319961178 28.57327725655128 86-87 28.87196637592082 21.652280019885207 20.17670719031048 29.299046413883488 88-89 28.908586853432556 20.759465966294595 19.333187422484862 30.99875975778799 90-91 24.052184572279135 23.281674109097278 22.327291830837932 30.33884948778566 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.5 9 2.5 10 2.5 11 4.5 12 10.0 13 12.5 14 16.0 15 26.0 16 46.0 17 70.0 18 102.5 19 136.0 20 170.5 21 222.0 22 291.0 23 385.5 24 520.5 25 683.5 26 878.5 27 1130.5 28 1410.0 29 1699.5 30 2076.0 31 2612.5 32 3204.0 33 3767.0 34 4539.0 35 5489.5 36 6527.5 37 7610.0 38 8719.0 39 10074.0 40 11589.5 41 13179.0 42 14758.0 43 16286.0 44 17867.0 45 19454.0 46 20960.0 47 22465.5 48 23885.0 49 25007.5 50 25986.5 51 26947.0 52 27644.0 53 28201.5 54 28583.0 55 28695.5 56 28641.0 57 28334.0 58 27862.5 59 27337.0 60 26954.0 61 26532.0 62 25810.0 63 24626.0 64 23169.5 65 21645.0 66 19735.5 67 17886.0 68 15982.0 69 13813.5 70 11825.5 71 10077.5 72 8426.0 73 6796.5 74 5304.0 75 4140.5 76 3246.0 77 2430.0 78 1825.0 79 1317.0 80 891.0 81 618.0 82 425.5 83 285.5 84 198.0 85 136.5 86 90.0 87 65.0 88 46.0 89 25.5 90 15.5 91 11.0 92 10.0 93 10.0 94 6.5 95 3.5 96 2.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.3622847072774905E-4 2 0.006141940238921475 3 7.086854121832471E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 6304.0 32-33 13647.0 34-35 14876.0 36-37 15618.0 38-39 16699.0 40-41 17462.0 42-43 17563.0 44-45 18088.0 46-47 18057.0 48-49 17820.0 50-51 17827.0 52-53 17670.0 54-55 16917.0 56-57 16632.0 58-59 15976.0 60-61 15545.0 62-63 15351.0 64-65 14252.0 66-67 13792.0 68-69 13241.0 70-71 12765.0 72-73 12117.0 74-75 11384.0 76-77 11110.0 78-79 10259.0 80-81 9809.0 82-83 9325.0 84-85 9004.0 86-87 8467.0 88-89 8098.0 90-91 7644.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.69073734191186 #Duplication Level Percentage of deduplicated Percentage of total 1 99.70118177877652 99.39284325386217 2 0.2893003357779191 0.5768112757392686 3 0.00854317715136238 0.025550268883856694 4 3.59047181609127E-4 0.0014317471310059682 5 3.2007811433898257E-4 0.001595441161273097 6 2.955829982419281E-4 0.0017680132224282509 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0011811423536387453 0.0 0.0 0.0 0.0 2 0.0011811423536387453 0.0 0.0 0.0 0.0 3 0.0011811423536387453 0.0 0.0 0.0 0.0 4 0.0011811423536387453 0.0 0.0 0.0 0.0 5 0.0011811423536387453 0.0 0.0 0.0 0.0 6 0.0011811423536387453 0.0 0.0 0.0 0.0 7 0.0014173708243664944 0.0 0.0 0.0 0.0 8 0.006614397180376973 0.0 0.0 0.0 0.0 9 0.006614397180376973 0.0 0.0 0.0 0.0 10-11 0.006614397180376973 0.0 0.0 0.0 0.0 12-13 0.006614397180376973 0.0 0.0 0.0 1.1811423536387452E-4 14-15 0.006614397180376973 0.0 0.0 0.0 2.3622847072774905E-4 16-17 0.006850625651104722 0.0 0.0 0.0 2.3622847072774905E-4 18-19 0.006850625651104722 0.0 0.0 0.0 2.3622847072774905E-4 20-21 0.006850625651104722 0.0 0.0 0.0 2.3622847072774905E-4 22-23 0.007086854121832471 0.0 0.0 0.0 2.3622847072774905E-4 24-25 0.007204968357196346 0.0 0.0 0.0 2.3622847072774905E-4 26-27 0.00732308259256022 0.0 0.0 0.0 2.3622847072774905E-4 28-29 0.00732308259256022 0.0 0.0 0.0 3.5434270609162354E-4 30-31 0.00732308259256022 0.0 0.0 1.1811423536387452E-4 4.724569414554981E-4 32-33 0.00732308259256022 0.0 0.0 2.3622847072774905E-4 7.086854121832472E-4 34-35 0.00732308259256022 0.0 0.0 2.3622847072774905E-4 7.086854121832472E-4 36-37 0.00732308259256022 0.0 0.0 2.3622847072774905E-4 7.086854121832472E-4 38-39 0.0075593110632879695 0.0 0.0 2.3622847072774905E-4 7.086854121832472E-4 40-41 0.007913653769379593 0.0 0.0 2.3622847072774905E-4 7.086854121832472E-4 42-43 0.008149882240107341 0.0 0.0 2.3622847072774905E-4 7.086854121832472E-4 44-45 0.008386110710835091 1.1811423536387452E-4 0.0 2.3622847072774905E-4 7.086854121832472E-4 46-47 0.008504224946198966 2.3622847072774905E-4 0.0 2.3622847072774905E-4 7.086854121832472E-4 48-49 0.008504224946198966 2.3622847072774905E-4 0.0 2.3622847072774905E-4 9.449138829109962E-4 50-51 0.008504224946198966 2.3622847072774905E-4 0.0 2.3622847072774905E-4 9.449138829109962E-4 52-53 0.008504224946198966 2.3622847072774905E-4 0.0 2.3622847072774905E-4 9.449138829109962E-4 54-55 0.008504224946198966 2.3622847072774905E-4 0.0 2.3622847072774905E-4 9.449138829109962E-4 56-57 0.008504224946198966 2.3622847072774905E-4 0.0 2.3622847072774905E-4 9.449138829109962E-4 58-59 0.00862233918156284 2.3622847072774905E-4 0.0 2.3622847072774905E-4 9.449138829109962E-4 60-61 0.008740453416926714 2.3622847072774905E-4 0.0 2.3622847072774905E-4 9.449138829109962E-4 62-63 0.008976681887654464 2.3622847072774905E-4 0.0 2.3622847072774905E-4 9.449138829109962E-4 64-65 0.009212910358382212 2.3622847072774905E-4 0.0 2.3622847072774905E-4 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