##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-7C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 561248 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.23422444267062 40.0 40.0 40.0 40.0 40.0 2 39.13554079480016 40.0 40.0 40.0 40.0 40.0 3 39.134617851644904 40.0 40.0 40.0 40.0 40.0 4 39.05869775927932 40.0 40.0 40.0 40.0 40.0 5 39.22045156508353 40.0 40.0 40.0 40.0 40.0 6 39.255281087861334 40.0 40.0 40.0 40.0 40.0 7 39.28651861565654 40.0 40.0 40.0 40.0 40.0 8 39.310066138320316 40.0 40.0 40.0 40.0 40.0 9 39.32747733622213 40.0 40.0 40.0 40.0 40.0 10-11 39.321679542733335 40.0 40.0 40.0 40.0 40.0 12-13 39.32196105821313 40.0 40.0 40.0 40.0 40.0 14-15 39.33300074120531 40.0 40.0 40.0 40.0 40.0 16-17 39.46103326871543 40.0 40.0 40.0 40.0 40.0 18-19 39.48047209076914 40.0 40.0 40.0 40.0 40.0 20-21 39.49122313130737 40.0 40.0 40.0 40.0 40.0 22-23 39.48448814071497 40.0 40.0 40.0 40.0 40.0 24-25 39.482909515935916 40.0 40.0 40.0 40.0 40.0 26-27 39.46939320941901 40.0 40.0 40.0 40.0 40.0 28-29 39.4707116996408 40.0 40.0 40.0 40.0 40.0 30-31 39.4599541715017 40.0 40.0 40.0 40.0 40.0 32-33 39.460194403295276 40.0 40.0 40.0 40.0 40.0 34-35 39.452776368793685 40.0 40.0 40.0 40.0 40.0 36-37 39.43969265466208 40.0 40.0 40.0 40.0 40.0 38-39 39.43184033505972 40.0 40.0 40.0 40.0 40.0 40-41 39.41952914636291 40.0 40.0 40.0 40.0 40.0 42-43 39.41691090771637 40.0 40.0 40.0 40.0 40.0 44-45 39.39836859469603 40.0 40.0 40.0 40.0 40.0 46-47 39.38235354146567 40.0 40.0 40.0 40.0 40.0 48-49 39.384860997773345 40.0 40.0 40.0 40.0 40.0 50-51 39.378056593569866 40.0 40.0 40.0 40.0 40.0 52-53 39.36215160583609 40.0 40.0 40.0 40.0 40.0 54-55 39.347298061908674 40.0 40.0 40.0 40.0 40.0 56-57 39.34051046064299 40.0 40.0 40.0 40.0 40.0 58-59 39.322814903566915 40.0 40.0 40.0 40.0 40.0 60-61 39.28709707271191 40.0 40.0 40.0 40.0 40.0 62-63 39.362346170364575 40.0 40.0 40.0 40.0 40.0 64-65 39.3482650401747 40.0 40.0 40.0 40.0 40.0 66-67 39.34394143540696 40.0 40.0 40.0 40.0 40.0 68-69 39.34756272497747 40.0 40.0 40.0 40.0 40.0 70-71 39.33351700014522 40.0 40.0 40.0 40.0 40.0 72-73 39.33223439406255 40.0 40.0 40.0 40.0 40.0 74-75 39.31846369230455 40.0 40.0 40.0 40.0 40.0 76-77 39.31730285443014 40.0 40.0 40.0 40.0 40.0 78-79 39.31396374913306 40.0 40.0 40.0 40.0 40.0 80-81 39.3045631184798 40.0 40.0 40.0 40.0 40.0 82-83 39.30320200820782 40.0 40.0 40.0 40.0 40.0 84-85 39.299440387054545 40.0 40.0 40.0 40.0 40.0 86-87 39.32641497733626 40.0 40.0 40.0 40.0 40.0 88-89 39.34977945052886 40.0 40.0 40.0 40.0 40.0 90-91 39.43097349589905 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2464745336880938 1101 2 0.09826898230768677 1101 3 0.09951679600647623 1101 4 0.15193984186644371 1101 5 0.20098001997924086 1101 6 -0.0155775894126009 1101 7 0.015723173790370026 1101 8 -0.06690864576023614 1101 9 0.011984584154866695 1101 10-11 0.04071873463207254 1101 12-13 -0.01584109550940127 1101 14-15 -0.03638746167987961 1101 16-17 0.07679463147841403 1101 18-19 0.020621070863342084 1101 20-21 0.06808679800305839 1101 22-23 0.058146943194550715 1101 24-25 -0.02293831102261379 1101 26-27 0.06719545689311701 1101 28-29 0.15342799264374918 1101 30-31 -0.015269300678845354 1101 32-33 0.10378739821275929 1101 34-35 0.006327907018501833 1101 36-37 0.08683691559809859 1101 38-39 0.16277231910449785 1101 40-41 -0.06935409808959037 1101 42-43 0.09809109051267484 1101 44-45 0.012964069585535754 1101 46-47 0.025907095914831757 1101 48-49 0.13364602761588174 1101 50-51 0.18700114504625276 1101 52-53 0.15431025498224216 1101 54-55 0.19140047984593878 1101 56-57 -0.028066061843937007 1101 58-59 0.15708595184842267 1101 60-61 0.09321412225953907 1101 62-63 -0.16202563021199978 1101 64-65 0.016701747349515017 1101 66-67 -0.07920883061327544 1101 68-69 -0.4002818023866297 1101 70-71 -0.2370032980297978 1101 72-73 -0.4211855238088873 1101 74-75 -0.41593792919368155 1101 76-77 -0.08624174050186184 1101 78-79 0.024967132282313287 1101 80-81 -0.4494904380524787 1101 82-83 -0.13043900905235972 1101 84-85 -0.19319164851572168 1101 86-87 -0.013641367425613282 1101 88-89 -0.6621663452685524 1101 90-91 0.6647788720207259 1102 1 0.31620878533471597 1102 2 0.12546779975041034 1102 3 0.19934215950548406 1102 4 -0.02627049373651147 1102 5 0.062495236901320084 1102 6 -0.04596303244643707 1102 7 -0.04122271505709563 1102 8 -0.12320831414709943 1102 9 0.11241561581836379 1102 10-11 -0.016839363072790547 1102 12-13 -0.0985141458923593 1102 14-15 -0.15601673520511383 1102 16-17 -0.05502256923583815 1102 18-19 -0.004712340958306527 1102 20-21 -0.17670125084283939 1102 22-23 0.02110830732146951 1102 24-25 0.03749899970608794 1102 26-27 -0.04223164381392763 1102 28-29 -0.1321805207221587 1102 30-31 -0.033486438602444935 1102 32-33 -0.15153563984591756 1102 34-35 -0.11648887257387486 1102 36-37 0.07283118030383662 1102 38-39 -0.07721569312990084 1102 40-41 0.12722096951154782 1102 42-43 -0.0070717322915143654 1102 44-45 -0.08744077837899766 1102 46-47 -0.13828244388842137 1102 48-49 -0.25676328074963806 1102 50-51 -0.14143883758197262 1102 52-53 -0.06737381775707263 1102 54-55 -0.2092813652343679 1102 56-57 -0.1348866326460083 1102 58-59 0.02000702296398771 1102 60-61 -0.012563770889933323 1102 62-63 -0.1879139166162389 1102 64-65 0.17510502271782968 1102 66-67 -0.2438610144926585 1102 68-69 0.0451811203108079 1102 70-71 -0.09046910817256304 1102 72-73 -0.13690276237673515 1102 74-75 0.09531774451080821 1102 76-77 -0.05099906459632564 1102 78-79 -0.031947663537096105 1102 80-81 0.2536311313821642 1102 82-83 -0.3877830641097404 1102 84-85 -0.7263137481822497 1102 86-87 -0.37293924396232825 1102 88-89 0.4489447658425547 1102 90-91 -1.335221127979274 1103 1 0.2707626606273337 1103 2 0.11361009224052765 1103 3 0.031326230042843406 1103 4 -0.11401986120885255 1103 5 -0.018618581803877987 1103 6 -0.20443113012191816 1103 7 -0.07082488009596233 1103 8 -0.08522685792786433 1103 9 -0.10061115049813196 1103 10-11 -0.023850606713516243 1103 12-13 -0.17483720720964868 1103 14-15 -0.053629518158416545 1103 16-17 -0.10542916177401906 1103 18-19 -0.111416162552473 1103 20-21 -0.021053455718323733 1103 22-23 0.014268069623206259 1103 24-25 -0.06273250606601266 1103 26-27 -0.01853566128762907 1103 28-29 -0.11983700087574789 1103 30-31 -0.016243339404745427 1103 32-33 -0.04171104015353677 1103 34-35 -0.05168669019480632 1103 36-37 -0.07870609413095053 1103 38-39 -0.07107215124903377 1103 40-41 -0.08042089155750887 1103 42-43 -0.22777473157444916 1103 44-45 -0.0894558535207679 1103 46-47 -0.11493605236718452 1103 48-49 0.07155664767087444 1103 50-51 -0.09350876062421065 1103 52-53 0.06008971512027017 1103 54-55 -0.17950793462131287 1103 56-57 -0.20796934569458614 1103 58-59 -0.062292912528590705 1103 60-61 -0.28505081520721376 1103 62-63 -0.22172352283106989 1103 64-65 -0.16201243426731082 1103 66-67 -0.1264568325436315 1103 68-69 -0.05733912422729048 1103 70-71 -0.25974695940347203 1103 72-73 0.0374463433200134 1103 74-75 -0.3102707032198211 1103 76-77 -0.23720541431094944 1103 78-79 -0.30092482017813893 1103 80-81 0.11178026194508561 1103 82-83 0.13743839318180306 1103 84-85 -0.2020656278815025 1103 86-87 -0.11198686300994609 1103 88-89 -0.2641271295822847 1103 90-91 -0.47807827083641286 1104 1 0.33712964873345186 1104 2 0.10525017953278848 1104 3 0.15234662932411425 1104 4 0.25110799957118246 1104 5 0.1046997676014243 1104 6 0.1351208442126648 1104 7 0.18103691959192503 1104 8 -0.003512845779070517 1104 9 0.06154355078700746 1104 10-11 0.011588559160436773 1104 12-13 -0.035773920320274044 1104 14-15 -0.05298663925466229 1104 16-17 -0.0847836715633079 1104 18-19 -0.03918368162433694 1104 20-21 -0.024081328311410743 1104 22-23 0.09660630848318874 1104 24-25 0.06479750842052567 1104 26-27 0.006894738055812866 1104 28-29 -0.03734954447524785 1104 30-31 -0.028566221292841476 1104 32-33 0.10243241800313285 1104 34-35 -0.02239081967858425 1104 36-37 -0.05691306123438267 1104 38-39 -0.023069848530077763 1104 40-41 0.06399750455389608 1104 42-43 -0.07392501461169587 1104 44-45 0.07688337637514309 1104 46-47 -0.14283534446578017 1104 48-49 0.011034691828996301 1104 50-51 -0.0023270098781082993 1104 52-53 -0.06771995970309774 1104 54-55 -0.12040970589958988 1104 56-57 -0.006570929293992833 1104 58-59 -0.12545018627118765 1104 60-61 -0.017489172791371743 1104 62-63 0.013018930971263387 1104 64-65 -0.12151170145439494 1104 66-67 -0.04366706087616734 1104 68-69 -0.04203964331276211 1104 70-71 -0.05023940992025189 1104 72-73 -0.12599438697516518 1104 74-75 -0.13214513052081145 1104 76-77 -0.20884902124867466 1104 78-79 -0.12028274087852253 1104 80-81 0.0050085593838034015 1104 82-83 -0.09923335107601616 1104 84-85 0.5329993071834309 1104 86-87 -0.24027817953655273 1104 88-89 -0.358510431290064 1104 90-91 -0.904599118409898 1201 1 0.1780935602856033 1201 2 0.264505232905492 1201 3 0.15717783415531983 1201 4 0.18317995517255525 1201 5 0.16332413210814423 1201 6 0.2555771764337038 1201 7 0.08514891782157008 1201 8 0.006990482371321605 1201 9 0.17280568557855247 1201 10-11 0.021441980778568848 1201 12-13 0.04766918665225006 1201 14-15 -0.005706521386308339 1201 16-17 -0.02318697467249109 1201 18-19 0.14888505938557728 1201 20-21 0.07736465548751426 1201 22-23 -0.017253123023301953 1201 24-25 -0.06923930796923372 1201 26-27 0.022079023828332822 1201 28-29 0.13746948916731583 1201 30-31 0.04681361879288204 1201 32-33 -0.030594814837144213 1201 34-35 -0.036715955876523765 1201 36-37 -0.03392834642379228 1201 38-39 -0.057109924732493766 1201 40-41 0.015651016674958385 1201 42-43 0.02834540525197582 1201 44-45 -0.03126992397479711 1201 46-47 0.09033985366019692 1201 48-49 -8.621315725605427E-4 1201 50-51 -0.006994138346229306 1201 52-53 0.05896960680153995 1201 54-55 0.24471594942794184 1201 56-57 0.10291148087704727 1201 58-59 -0.18888634890953426 1201 60-61 0.09421760159949599 1201 62-63 -0.10324920972092855 1201 64-65 -0.09240335061465998 1201 66-67 0.047648135690401716 1201 68-69 0.1373878685886325 1201 70-71 -0.16714240979340644 1201 72-73 -0.1286228218616685 1201 74-75 0.04042548635222687 1201 76-77 0.11500390907752234 1201 78-79 -0.03956531016109466 1201 80-81 -0.07285707133843289 1201 82-83 -0.1749889980885868 1201 84-85 -0.4797427961820375 1201 86-87 -0.7126926612767193 1201 88-89 -0.034715364876397814 1201 90-91 -0.062493855252000685 1202 1 0.07186837219621367 1202 2 0.07825981485696332 1202 3 0.21934020195566717 1202 4 -0.011882502588456134 1202 5 -0.06061077397024661 1202 6 0.20168134282064187 1202 7 0.12525973503135646 1202 8 0.1548616372244922 1202 9 0.006199440250014732 1202 10-11 0.045724645225313054 1202 12-13 0.021137505730614237 1202 14-15 -0.016786004537706845 1202 16-17 0.012873375055164615 1202 18-19 0.021434078993422645 1202 20-21 -0.04170789935044894 1202 22-23 0.09235118434675371 1202 24-25 0.05470275915663336 1202 26-27 -0.09578734207433826 1202 28-29 0.04755358671867782 1202 30-31 0.05651907595862582 1202 32-33 -0.08625455603230137 1202 34-35 -0.01641547815540889 1202 36-37 -0.016872052904076895 1202 38-39 0.03837823591861422 1202 40-41 -0.030639519145836402 1202 42-43 -0.0814603503318807 1202 44-45 -0.030202655317957294 1202 46-47 0.07934678025966946 1202 48-49 -0.020511076943364515 1202 50-51 -0.02481759624570401 1202 52-53 -0.10047153394967268 1202 54-55 0.012779223088429603 1202 56-57 -0.03464952255806253 1202 58-59 -0.012841936909538276 1202 60-61 -0.12592728857013924 1202 62-63 0.1952189815292371 1202 64-65 -0.00998712587903583 1202 66-67 0.1413503191601464 1202 68-69 0.22553299264025384 1202 70-71 0.16070937271260988 1202 72-73 -0.009145510448874461 1202 74-75 0.0095019978144677 1202 76-77 0.26843227856783614 1202 78-79 -0.16728146373931452 1202 80-81 -0.40308992533889665 1202 82-83 -0.013237813803137044 1202 84-85 -0.3401608659767419 1202 86-87 0.36896551794243493 1202 88-89 0.6711669880647761 1202 90-91 0.6647788720207259 1203 1 0.23010421387296986 1203 2 0.22534730169176953 1203 3 0.1976596890701643 1203 4 0.24857645477160162 1203 5 -0.03066623168209759 1203 6 0.046819287869872994 1203 7 0.09138202168960419 1203 8 0.049920263324146674 1203 9 0.09961225277323393 1203 10-11 0.02396765401376655 1203 12-13 0.03640694979873871 1203 14-15 0.07375777821140161 1203 16-17 0.031555937559808456 1203 18-19 0.04131625866941846 1203 20-21 0.043346924683852706 1203 22-23 0.07485731353831682 1203 24-25 0.125912821082224 1203 26-27 -0.029342972408699097 1203 28-29 0.07914132046921907 1203 30-31 0.04248421058233731 1203 32-33 0.18600162381012808 1203 34-35 0.07740127690841092 1203 36-37 0.15401321090018172 1203 38-39 0.02405888999953021 1203 40-41 -0.006618712118495296 1203 42-43 0.05273553997341196 1203 44-45 -0.023267161841388884 1203 46-47 0.06112505182647965 1203 48-49 2.705879467868044E-4 1203 50-51 -0.004667780284101752 1203 52-53 0.04017026507299448 1203 54-55 0.07646309401553708 1203 56-57 0.009815001421920044 1203 58-59 -0.0179242551605725 1203 60-61 -0.07212639036508506 1203 62-63 0.11584190445406506 1203 64-65 -0.014682052493810716 1203 66-67 0.05887149152207627 1203 68-69 -0.10410775510710835 1203 70-71 0.10603447577958036 1203 72-73 -0.03337326320334455 1203 74-75 0.2540574945694658 1203 76-77 0.3351616422127819 1203 78-79 0.32465459379997696 1203 80-81 0.4622316015704371 1203 82-83 0.08014158796186166 1203 84-85 0.4422072163913384 1203 86-87 -0.020821017361342342 1203 88-89 0.38545270235049145 1203 90-91 -0.33522112797927406 1204 1 0.22771776821675616 1204 2 0.11902947886449766 1204 3 -2.2755554469000572E-4 1204 4 -0.007561758815285202 1204 5 -0.140317568634039 1204 6 0.05186753829647017 1204 7 -0.16768501156627025 1204 8 -0.08371234920803516 1204 9 -0.12703750294985383 1204 10-11 -0.06666512957483661 1204 12-13 -0.15796320794865437 1204 14-15 -0.11697430154261212 1204 16-17 -0.127088461176875 1204 18-19 -0.08196535443434527 1204 20-21 -0.07766506178182908 1204 22-23 -0.056970862374434716 1204 24-25 -0.15485751690022198 1204 26-27 -0.027882734682002308 1204 28-29 0.04849927480132976 1204 30-31 -0.12646946650486512 1204 32-33 -0.28056372445038136 1204 34-35 -0.07840461950882371 1204 36-37 0.017331258274900563 1204 38-39 -0.019623036654145665 1204 40-41 -0.06565100450951888 1204 42-43 -0.03933583081352765 1204 44-45 0.020748652437980297 1204 46-47 -0.03595282787067333 1204 48-49 -0.19219551436651727 1204 50-51 -0.10936017636898754 1204 52-53 -0.013136626732162426 1204 54-55 -0.013065278418856963 1204 56-57 0.123286876891612 1204 58-59 -0.09576967804590453 1204 60-61 -0.09047655532857846 1204 62-63 0.037186547740098774 1204 64-65 0.0326239256442733 1204 66-67 0.11596512752839772 1204 68-69 -0.07293558548953172 1204 70-71 -0.028421610233600347 1204 72-73 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2401 50-51 0.10351204382700985 2401 52-53 0.0499046551462925 2401 54-55 0.17537174156979773 2401 56-57 0.10493728427109517 2401 58-59 -0.05528413203504812 2401 60-61 -0.04726141733629419 2401 62-63 0.14032369713654447 2401 64-65 0.1541440936997276 2401 66-67 0.19211773331594628 2401 68-69 0.1428962698497216 2401 70-71 0.17842605451379967 2401 72-73 0.2145494256645719 2401 74-75 0.10331428029765277 2401 76-77 0.18629974312428033 2401 78-79 0.0035691109733306803 2401 80-81 0.1173479430872959 2401 82-83 0.41617015348846564 2401 84-85 0.3614922542877608 2401 86-87 0.3590195677313659 2401 88-89 0.29907396480896153 2401 90-91 0.31695278506420266 2402 1 0.30056412827531886 2402 2 0.28982106393588936 2402 3 0.32646883398322757 2402 4 0.19166538341715977 2402 5 0.13471712916241074 2402 6 0.1304746831906627 2402 7 0.1642635786575113 2402 8 0.2508029273286496 2402 9 0.13293454868806975 2402 10-11 0.1558903393682627 2402 12-13 0.11838572830246363 2402 14-15 0.21513351829502625 2402 16-17 0.08939489338707318 2402 18-19 0.015561595771941938 2402 20-21 0.124509016587659 2402 22-23 0.0988982936610796 2402 24-25 0.027545947175667607 2402 26-27 0.11712315919027816 2402 28-29 0.09589266225688675 2402 30-31 0.12021523034096049 2402 32-33 0.1023401658983829 2402 34-35 0.08941714945871837 2402 36-37 0.009741714785675981 2402 38-39 0.07942188363436031 2402 40-41 0.00797431147144323 2402 42-43 0.09806808031377301 2402 44-45 0.1080513397114089 2402 46-47 0.13363486281125603 2402 48-49 0.1764506267844581 2402 50-51 0.1895980065969738 2402 52-53 0.17170241432278033 2402 54-55 0.08583068430355922 2402 56-57 0.049507524271454884 2402 58-59 0.16819767536988905 2402 60-61 0.23196639973058097 2402 62-63 0.1734582495955692 2402 64-65 0.19160059359641934 2402 66-67 0.08459230802917972 2402 68-69 0.18413832807306107 2402 70-71 0.1305556869789939 2402 72-73 0.2535169433012854 2402 74-75 0.10619799983511768 2402 76-77 0.3699212154925746 2402 78-79 0.2778862735137295 2402 80-81 0.2481488035697339 2402 82-83 0.08056023661733747 2402 84-85 0.26613360583497325 2402 86-87 -0.25585410687718735 2402 88-89 -0.15349155752399923 2402 90-91 -0.6172724100305587 2403 1 -0.23234537470722927 2403 2 -0.22330199137424955 2403 3 -0.08644335795320046 2403 4 -0.05545633839135178 2403 5 0.0912219281219464 2403 6 0.10747315213324526 2403 7 0.1130554700896198 2403 8 0.23121472140965693 2403 9 0.10784907836808344 2403 10-11 0.11876219699637858 2403 12-13 0.09211149341959413 2403 14-15 0.16965658567801256 2403 16-17 0.1484935725070713 2403 18-19 0.09297945920601336 2403 20-21 0.07374129281456021 2403 22-23 0.08332293984413752 2403 24-25 0.11619842458022589 2403 26-27 0.04575544200236692 2403 28-29 0.12751942448202414 2403 30-31 0.018286729076052666 2403 32-33 0.11149276533957675 2403 34-35 0.11634498810889227 2403 36-37 0.08123328499920035 2403 38-39 0.05417601325887489 2403 40-41 0.05091595755187228 2403 42-43 0.12064107675696079 2403 44-45 0.07316463286404229 2403 46-47 0.09771937818813115 2403 48-49 0.09071655166515313 2403 50-51 0.08997964617834242 2403 52-53 0.10498375516613123 2403 54-55 -0.083520044918302 2403 56-57 0.09973468728059487 2403 58-59 0.052310980007156616 2403 60-61 0.04624842764457071 2403 62-63 0.3429804872241746 2403 64-65 0.30665459327585154 2403 66-67 0.10414417672119924 2403 68-69 0.27076363397684133 2403 70-71 0.17158448983632724 2403 72-73 0.3015139902390871 2403 74-75 0.28568798453820676 2403 76-77 0.23220406556718842 2403 78-79 0.1548666020127598 2403 80-81 0.03708804792933762 2403 82-83 -0.15956508764199384 2403 84-85 0.2969432037940791 2403 86-87 0.50289586450549 2403 88-89 0.29021460711239655 2403 90-91 0.4485626558045084 2404 1 -0.3312023038090217 2404 2 -0.22580198309832156 2404 3 -0.4074645410133755 2404 4 -0.34358038459088647 2404 5 -0.23705691099736015 2404 6 -0.18729017293519234 2404 7 -0.17411916187995047 2404 8 0.049572049411089836 2404 9 0.08437193151011968 2404 10-11 0.03849365974975427 2404 12-13 0.004039751072347997 2404 14-15 0.09849289117223492 2404 16-17 -0.012787673251516196 2404 18-19 0.022648653892204607 2404 20-21 0.004283763211574865 2404 22-23 -0.017729426905177093 2404 24-25 -0.023148968999635144 2404 26-27 -0.03296582812054538 2404 28-29 -0.054735003965973306 2404 30-31 -0.005690945785985946 2404 32-33 0.07266097117067005 2404 34-35 -0.02471506348414465 2404 36-37 0.07826825353154732 2404 38-39 0.08274826264293722 2404 40-41 0.05856596117200752 2404 42-43 0.06859210630837254 2404 44-45 -0.012680145312579327 2404 46-47 -0.01263924764476343 2404 48-49 -0.03257648671867486 2404 50-51 0.08385315500689927 2404 52-53 0.09567444380230938 2404 54-55 0.07881377816420354 2404 56-57 0.07000699412979117 2404 58-59 -0.05462004034595935 2404 60-61 0.013359115995427828 2404 62-63 0.17187614454639544 2404 64-65 0.039731280908924305 2404 66-67 -0.0012638731292966554 2404 68-69 -0.02485906398720772 2404 70-71 0.17874723044005947 2404 72-73 -0.026956973696442788 2404 74-75 -0.1569193867117491 2404 76-77 -2.44088505809259E-4 2404 78-79 0.3429039593781056 2404 80-81 0.23479441623683073 2404 82-83 0.1788171605463802 2404 84-85 0.1930653780441105 2404 86-87 0.17950943822112464 2404 88-89 -0.3316820147842279 2404 90-91 0.024778872020725373 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 18.0 31 314.0 32 2395.0 33 4174.0 34 5575.0 35 6854.0 36 9980.0 37 15476.0 38 41295.0 39 242644.0 40 232523.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 25.194792236462543 12.07328785063048 31.76454237855887 30.967377534348113 2 26.72536064545558 18.323069905348387 28.261667521952333 26.689901927243696 3 26.89915312069589 20.233519885538552 22.678298116481983 30.18902887728357 4 26.175059866583045 21.094418153828613 21.580834141057075 31.149687838531275 5 27.08677803751639 21.255844118820914 21.554286162266948 30.103091681395743 6 27.783617937168597 23.33228804378813 24.007212497861907 24.876881521181367 7 25.926150293631334 23.065739209761105 23.575852386110952 27.432258110496612 8 25.124722048007296 23.845430184161014 23.894249957238156 27.135597810593538 9 25.248018701180225 24.30476366953646 24.153137294030447 26.29408033525287 10-11 24.79171417982781 23.064313814926734 24.12703475112606 28.01693725411939 12-13 25.321248360795938 22.975315724955813 23.8807977934888 27.822638120759454 14-15 25.64579294714636 22.701105393694053 23.60908190318718 28.044019755972403 16-17 25.68606035121729 22.352061833627914 23.31322338787844 28.648654427276355 18-19 26.041161839329497 22.679457209647072 23.12631849022179 28.153062460801642 20-21 26.016751952790923 22.934246536290555 22.988678801528025 28.0603227093905 22-23 25.692207366440506 22.3989216888078 23.17246564798449 28.736405296767202 24-25 25.874925166771195 22.89184103996807 23.25059510234335 27.982638690917383 26-27 25.88027039740008 22.914201921432237 23.241151861565655 27.964375819602026 28-29 25.897018786703917 22.477674753406692 23.37193183761902 28.253374622270368 30-31 26.135110335058663 22.774456788033746 23.352724745877467 27.73770813103012 32-33 26.066563590195408 22.797150424529892 23.395929360121066 27.740356625153634 34-35 25.858512373641474 22.38441263250742 23.60947899812188 28.14759599572923 36-37 26.05371639786921 22.75753186246424 23.584050991695634 27.604700747970917 38-39 26.07014354468146 22.863777232232778 23.44811931825734 27.617959904828414 40-41 25.96306809654693 22.386294932100697 23.683178038960094 27.967458932392276 42-43 26.10673553121498 22.79375881140874 23.62960000722987 27.469905650146405 44-45 26.13784492923823 22.66577053430696 23.634334944617585 27.562049591837223 46-47 26.030648835881042 22.303789063145967 23.686637468818812 27.978924632154182 48-49 26.224492155985086 22.584858350600463 23.713944809589957 27.476704683824497 50-51 26.253914029430288 22.74367070574535 23.553142401267102 27.449272863557262 52-53 26.090553526236004 22.313445301346075 23.626269725458805 27.969731446959116 54-55 26.345785334183486 22.68864755646649 23.638573453015677 27.32699365633435 56-57 26.435129681065746 22.57541458474507 23.526520764608204 27.46293496958098 58-59 26.122510512076662 22.35547931810033 23.653598552755955 27.86841161706705 60-61 26.38864253302229 22.738284749461695 23.60495845130349 27.268114266212525 62-63 26.452760705665728 22.547463236208912 23.366095200179153 27.633680857946203 64-65 26.161483233662608 22.33179523912645 23.414846773611412 28.091874753599527 66-67 26.480230835217967 22.594400686266862 23.43258207907666 27.492786399438508 68-69 26.567803801765105 22.545400543109302 23.299247567322922 27.58754808780267 70-71 26.327049070731377 22.28664503032734 23.513109433687053 27.873196465254228 72-73 26.485987353698036 22.482106279559208 23.393783719175225 27.63812264756753 74-75 26.81159817702192 22.308447452272965 23.17825588601451 27.701698484690603 76-77 26.70294393986846 21.987550892577513 23.299013466958975 28.010491700595054 78-79 26.62327403709438 22.528357926000822 23.047331669247054 27.801036367657744 80-81 27.17487206634904 22.010469972354567 22.767288198733407 28.047369762562983 82-83 27.11317556964409 21.767622873642583 22.883685895424016 28.235515661289313 84-85 27.71981865066658 21.73495356715487 22.029899044376656 28.515328737801898 86-87 28.049522048986937 20.956874954393985 21.895750735777007 29.09785226084207 88-89 27.94432116199314 19.95158361912447 21.63405285454912 30.470042364333267 90-91 24.30478867583576 21.65445236422046 24.144162232707558 29.89659672723622 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 0.5 8 0.5 9 1.0 10 1.5 11 2.5 12 5.5 13 12.0 14 20.0 15 29.0 16 41.5 17 70.0 18 108.0 19 142.5 20 210.5 21 329.0 22 467.0 23 643.0 24 877.5 25 1142.0 26 1470.5 27 1889.0 28 2373.0 29 2926.5 30 3546.0 31 4244.0 32 5010.5 33 5791.5 34 6697.0 35 7814.5 36 9096.0 37 10247.5 38 11533.5 39 13268.5 40 15019.0 41 16761.0 42 18514.5 43 20476.0 44 22863.5 45 25390.5 46 27955.5 47 30368.5 48 32500.5 49 34723.0 50 36898.0 51 38749.5 52 40114.5 53 40936.0 54 41559.5 55 41958.5 56 41710.0 57 40972.0 58 40074.5 59 38742.5 60 36983.0 61 34989.5 62 33151.0 63 31251.5 64 28892.0 65 26537.0 66 23834.0 67 21164.0 68 18767.0 69 16100.5 70 13683.5 71 11546.5 72 9524.5 73 7645.5 74 5922.0 75 4591.5 76 3526.5 77 2555.0 78 1837.5 79 1307.0 80 914.0 81 657.0 82 464.0 83 319.5 84 223.0 85 159.0 86 107.5 87 73.5 88 51.5 89 32.0 90 13.0 91 6.0 92 5.5 93 5.0 94 5.0 95 3.5 96 1.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0016035691886652603 2 0.005701579337476482 3 0.0019599178972575402 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 4.976461337871865E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 8131.0 32-33 17737.0 34-35 19684.0 36-37 21100.0 38-39 22396.0 40-41 23597.0 42-43 24160.0 44-45 25089.0 46-47 25549.0 48-49 25246.0 50-51 25127.0 52-53 24843.0 54-55 24116.0 56-57 23287.0 58-59 22916.0 60-61 21971.0 62-63 21218.0 64-65 19981.0 66-67 19014.0 68-69 18385.0 70-71 17059.0 72-73 15694.0 74-75 14887.0 76-77 13533.0 78-79 12554.0 80-81 11455.0 82-83 10438.0 84-85 9346.0 86-87 8404.0 88-89 7583.0 90-91 6748.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.69996083299165 #Duplication Level Percentage of deduplicated Percentage of total 1 99.71396263315245 99.41478169027693 2 0.2745913531487628 0.5475349430801966 3 0.010037072819429687 0.03002087300925075 4 6.569899214448771E-4 0.00262007477742898 5 2.8580219418586496E-4 0.0014247233783156905 6 2.5822624748111646E-4 0.001544708805595063 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 2.0792251621633295E-4 0.0020729866723065466 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0023162666058498204 0.0 0.0 0.0 0.0 2 0.0023162666058498204 0.0 0.0 0.0 0.0 3 0.0023162666058498204 0.0 0.0 0.0 0.0 4 0.0023162666058498204 0.0 0.0 0.0 0.0 5 0.0023162666058498204 0.0 0.0 0.0 0.0 6 0.0023162666058498204 0.0 0.0 0.0 0.0 7 0.0024944409601459602 0.0 0.0 0.0 0.0 8 0.006414276754661041 0.0 0.0 0.0 0.0 9 0.006414276754661041 0.0 0.0 0.0 0.0 10-11 0.006414276754661041 0.0 0.0 0.0 0.0 12-13 0.006414276754661041 0.0 0.0 0.0 0.0 14-15 0.006414276754661041 0.0 1.7817435429614003E-4 0.0 0.0 16-17 0.006414276754661041 0.0 1.7817435429614003E-4 0.0 0.0 18-19 0.006859712640401391 0.0 2.6726153144421E-4 0.0 0.0 20-21 0.0071269741718456014 0.0 3.5634870859228005E-4 8.908717714807001E-5 0.0 22-23 0.007394235703289811 0.0 3.5634870859228005E-4 1.7817435429614003E-4 0.0 24-25 0.007661497234734021 0.0 3.5634870859228005E-4 1.7817435429614003E-4 0.0 26-27 0.007839671589030161 0.0 3.5634870859228005E-4 1.7817435429614003E-4 0.0 28-29 0.008017845943326302 0.0 3.5634870859228005E-4 1.7817435429614003E-4 0.0 30-31 0.008374194651918581 1.7817435429614003E-4 3.5634870859228005E-4 1.7817435429614003E-4 1.7817435429614003E-4 32-33 0.008463281829066652 1.7817435429614003E-4 3.5634870859228005E-4 1.7817435429614003E-4 3.5634870859228005E-4 34-35 0.009086892069103143 1.7817435429614003E-4 3.5634870859228005E-4 1.7817435429614003E-4 3.5634870859228005E-4 36-37 0.009354153600547352 1.7817435429614003E-4 3.5634870859228005E-4 1.7817435429614003E-4 3.5634870859228005E-4 38-39 0.009710502309139632 1.7817435429614003E-4 3.5634870859228005E-4 1.7817435429614003E-4 3.5634870859228005E-4 40-41 0.009977763840583841 1.7817435429614003E-4 5.345230628884201E-4 1.7817435429614003E-4 3.5634870859228005E-4 42-43 0.009977763840583841 1.7817435429614003E-4 5.345230628884201E-4 1.7817435429614003E-4 3.5634870859228005E-4 44-45 0.010155938194879982 1.7817435429614003E-4 5.345230628884201E-4 1.7817435429614003E-4 3.5634870859228005E-4 46-47 0.010245025372028052 1.7817435429614003E-4 5.345230628884201E-4 1.7817435429614003E-4 3.5634870859228005E-4 48-49 0.010334112549176122 1.7817435429614003E-4 5.345230628884201E-4 1.7817435429614003E-4 3.5634870859228005E-4 50-51 0.010334112549176122 1.7817435429614003E-4 5.345230628884201E-4 1.7817435429614003E-4 3.5634870859228005E-4 52-53 0.010334112549176122 1.7817435429614003E-4 5.345230628884201E-4 1.7817435429614003E-4 5.345230628884201E-4 54-55 0.010423199726324193 1.7817435429614003E-4 5.345230628884201E-4 1.7817435429614003E-4 5.345230628884201E-4 56-57 0.010512286903472261 1.7817435429614003E-4 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