##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-7B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 679033 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.2582245634601 40.0 40.0 40.0 40.0 40.0 2 39.139396759804015 40.0 40.0 40.0 40.0 40.0 3 39.135588402920035 40.0 40.0 40.0 40.0 40.0 4 39.06895246622771 40.0 40.0 40.0 40.0 40.0 5 39.23117727709846 40.0 40.0 40.0 40.0 40.0 6 39.28147821976251 40.0 40.0 40.0 40.0 40.0 7 39.287457310616716 40.0 40.0 40.0 40.0 40.0 8 39.2984553033505 40.0 40.0 40.0 40.0 40.0 9 39.33410011000938 40.0 40.0 40.0 40.0 40.0 10-11 39.32060150243066 40.0 40.0 40.0 40.0 40.0 12-13 39.32093727403528 40.0 40.0 40.0 40.0 40.0 14-15 39.336662577518325 40.0 40.0 40.0 40.0 40.0 16-17 39.464803625155184 40.0 40.0 40.0 40.0 40.0 18-19 39.4768295502575 40.0 40.0 40.0 40.0 40.0 20-21 39.4898922438232 40.0 40.0 40.0 40.0 40.0 22-23 39.493824306035194 40.0 40.0 40.0 40.0 40.0 24-25 39.4899364242975 40.0 40.0 40.0 40.0 40.0 26-27 39.48011363217988 40.0 40.0 40.0 40.0 40.0 28-29 39.487459372372186 40.0 40.0 40.0 40.0 40.0 30-31 39.4766925030915 40.0 40.0 40.0 40.0 40.0 32-33 39.477926221299775 40.0 40.0 40.0 40.0 40.0 34-35 39.48292852901342 40.0 40.0 40.0 40.0 40.0 36-37 39.477857930373204 40.0 40.0 40.0 40.0 40.0 38-39 39.46517605648857 40.0 40.0 40.0 40.0 40.0 40-41 39.45388747100081 40.0 40.0 40.0 40.0 40.0 42-43 39.45357917983556 40.0 40.0 40.0 40.0 40.0 44-45 39.44039656491592 40.0 40.0 40.0 40.0 40.0 46-47 39.43226861412475 40.0 40.0 40.0 40.0 40.0 48-49 39.43732202696655 40.0 40.0 40.0 40.0 40.0 50-51 39.44050979766648 40.0 40.0 40.0 40.0 40.0 52-53 39.427488184374425 40.0 40.0 40.0 40.0 40.0 54-55 39.4008690103419 40.0 40.0 40.0 40.0 40.0 56-57 39.40711527071743 40.0 40.0 40.0 40.0 40.0 58-59 39.39192371582671 40.0 40.0 40.0 40.0 40.0 60-61 39.351631607638325 40.0 40.0 40.0 40.0 40.0 62-63 39.36825449709946 40.0 40.0 40.0 40.0 40.0 64-65 39.37185310511921 40.0 40.0 40.0 40.0 40.0 66-67 39.35950039258928 40.0 40.0 40.0 40.0 40.0 68-69 39.367248493913245 40.0 40.0 40.0 40.0 40.0 70-71 39.359128117406755 40.0 40.0 40.0 40.0 40.0 72-73 39.34857455126628 40.0 40.0 40.0 40.0 40.0 74-75 39.35813937118755 40.0 40.0 40.0 40.0 40.0 76-77 39.34937748383058 40.0 40.0 40.0 40.0 40.0 78-79 39.33988164590155 40.0 40.0 40.0 40.0 40.0 80-81 39.32468559394229 40.0 40.0 40.0 40.0 40.0 82-83 39.28592827840268 40.0 40.0 40.0 40.0 40.0 84-85 39.30534332952057 40.0 40.0 40.0 40.0 40.0 86-87 39.32684916348268 40.0 40.0 40.0 40.0 40.0 88-89 39.36390639884245 40.0 40.0 40.0 40.0 40.0 90-91 39.44207683073229 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.231241934189228 1101 2 0.1708229352808317 1101 3 0.09943559176227268 1101 4 0.24299919037014917 1101 5 0.06864824460907926 1101 6 0.012556919700593028 1101 7 -0.10786380147818875 1101 8 0.08737806145570914 1101 9 0.23364447254337506 1101 10-11 -0.020109857732663272 1101 12-13 0.07610304995255035 1101 14-15 -0.05566155269368522 1101 16-17 0.10161146722724368 1101 18-19 0.05006693051476674 1101 20-21 0.09910551356461639 1101 22-23 0.0393990528021817 1101 24-25 0.025261627388061925 1101 26-27 0.027358130172032702 1101 28-29 0.05495539171511865 1101 30-31 0.1450838499486906 1101 32-33 0.04086351511987374 1101 34-35 0.07557694485007715 1101 36-37 0.09598852451140516 1101 38-39 0.10151400615264805 1101 40-41 0.052932062668958224 1101 42-43 0.04313701397648373 1101 44-45 0.10149587455143205 1101 46-47 0.031146944571482038 1101 48-49 0.1344184162486357 1101 50-51 -0.007358627117739047 1101 52-53 4.247995814168348E-4 1101 54-55 0.13339829110810797 1101 56-57 0.21238738429920545 1101 58-59 0.09448981203102846 1101 60-61 0.11738177512962977 1101 62-63 0.06178451185040501 1101 64-65 0.12660805869474245 1101 66-67 0.16840140665374292 1101 68-69 0.14440122482258033 1101 70-71 0.10609609064682246 1101 72-73 0.1370341609135437 1101 74-75 -0.01845789310996082 1101 76-77 0.1719913063103249 1101 78-79 0.07009687976106704 1101 80-81 0.23675181257463862 1101 82-83 0.20853851769926024 1101 84-85 0.14797096124985387 1101 86-87 -0.3366792565469652 1101 88-89 -0.7372207147646463 1101 90-91 0.43541913397650944 1102 1 0.30897150107134763 1102 2 0.200877727663908 1102 3 0.2693309881218511 1102 4 0.1170637289558698 1102 5 0.03891452478840307 1102 6 0.0705750317939291 1102 7 0.2130347215880235 1102 8 5.532651761086527E-5 1102 9 0.02459322696187627 1102 10-11 -0.024324310198920784 1102 12-13 -0.004582184910532305 1102 14-15 -0.04155686503428058 1102 16-17 0.04711315060768584 1102 18-19 0.04241970224117608 1102 20-21 0.04174454998684496 1102 22-23 -0.026149261116771072 1102 24-25 -0.022005054882129116 1102 26-27 -0.051727743235353785 1102 28-29 0.011988681238555898 1102 30-31 -0.03063586270637586 1102 32-33 0.09201347873818833 1102 34-35 0.0884966853745226 1102 36-37 0.0030820368502730844 1102 38-39 -0.03635957247413302 1102 40-41 0.06248874734875898 1102 42-43 -0.10102540615991273 1102 44-45 0.1458445355297613 1102 46-47 -0.0015803521202286674 1102 48-49 0.03546483292699065 1102 50-51 -0.04234438850950539 1102 52-53 0.08263097253378504 1102 54-55 -0.05670276099392879 1102 56-57 -0.07865113005536273 1102 58-59 -0.03757618083994885 1102 60-61 -0.042022613967603206 1102 62-63 -0.047683957668326116 1102 64-65 -0.033109179147118084 1102 66-67 0.024564098887680075 1102 68-69 0.14280798665632943 1102 70-71 -0.25307242024172893 1102 72-73 0.12882046004418868 1102 74-75 -0.04172448159393838 1102 76-77 0.18719529859190231 1102 78-79 -0.02840835896156335 1102 80-81 0.06053384084491853 1102 82-83 -0.1746137105975336 1102 84-85 0.19094947199447887 1102 86-87 0.23791838160142476 1102 88-89 0.17756058236958694 1102 90-91 -0.17996548140810376 1103 1 0.17719854527656054 1103 2 0.024987135633551816 1103 3 -0.005893467638706795 1103 4 -0.07071442580765819 1103 5 0.03052434306736984 1103 6 -0.05916956077075497 1103 7 -0.11223790411934687 1103 8 0.06746086889501868 1103 9 0.00744781701686037 1103 10-11 0.01387898390418485 1103 12-13 0.044827153656115115 1103 14-15 0.07120791327724874 1103 16-17 0.0097370989830452 1103 18-19 -0.03385072950727164 1103 20-21 0.017931153516173026 1103 22-23 -0.027435019666469884 1103 24-25 0.09155311078863093 1103 26-27 0.02944652893477695 1103 28-29 0.014755233263933576 1103 30-31 0.03621695805064462 1103 32-33 0.10008099466441678 1103 34-35 0.01581960166735996 1103 36-37 0.04051202264976439 1103 38-39 0.03311017150811324 1103 40-41 1.756075044383465E-4 1103 42-43 -0.013857317283672899 1103 44-45 0.05869948856307872 1103 46-47 0.04174666189045695 1103 48-49 0.13217671821693955 1103 50-51 0.13278039450057832 1103 52-53 0.02814996416739035 1103 54-55 0.12644301500287014 1103 56-57 0.104334730690681 1103 58-59 0.10729467447892205 1103 60-61 0.13295844095709697 1103 62-63 -0.1796626510384769 1103 64-65 -0.19420410589920323 1103 66-67 -0.07936074540131699 1103 68-69 -0.27224272178215614 1103 70-71 -0.24392440720394148 1103 72-73 -0.06829777732675524 1103 74-75 0.08596114965232715 1103 76-77 -0.32199778913923893 1103 78-79 0.16589184599801854 1103 80-81 -0.059658240975061005 1103 82-83 -0.096769874633992 1103 84-85 -0.10422902341390738 1103 86-87 -0.42971052023320055 1103 88-89 0.34422724903625834 1103 90-91 0.054466753024129844 1104 1 0.27068612445824414 1104 2 0.19850983335264516 1104 3 0.20076670369825678 1104 4 0.14651659467310196 1104 5 0.040873584077154135 1104 6 0.07015642938179667 1104 7 0.15640479193415757 1104 8 0.06629636755390322 1104 9 -0.05335891422434713 1104 10-11 0.047403213118975884 1104 12-13 0.01198609856143662 1104 14-15 0.060956772683475435 1104 16-17 -0.1248933712178939 1104 18-19 0.021400977457609827 1104 20-21 -0.010456566146714863 1104 22-23 0.06625652368950341 1104 24-25 -0.05849253413515498 1104 26-27 -0.05349843143864774 1104 28-29 -0.07281667910486789 1104 30-31 -0.06709124910778996 1104 32-33 -0.04505541652412859 1104 34-35 -0.06670152125603579 1104 36-37 0.06714249657158433 1104 38-39 -0.09124727719395054 1104 40-41 0.0025567436158979717 1104 42-43 0.06870914918764015 1104 44-45 -0.0207884982942943 1104 46-47 0.016728331942047703 1104 48-49 -0.05764102797574111 1104 50-51 -0.010111361703664556 1104 52-53 -0.11564505767706379 1104 54-55 -0.024021653795223585 1104 56-57 0.0075448684941648025 1104 58-59 -0.059278643749536286 1104 60-61 -0.19570828269456797 1104 62-63 -0.07485857348029157 1104 64-65 -0.08796227198371298 1104 66-67 -0.10511790355909056 1104 68-69 -0.09892034973550068 1104 70-71 -0.06752903247667774 1104 72-73 -0.1325017979923686 1104 74-75 0.017008488926812504 1104 76-77 0.1967691475336082 1104 78-79 -0.07238627287651411 1104 80-81 -0.05549022193363129 1104 82-83 0.10189579495746415 1104 84-85 0.08736598352091107 1104 86-87 0.5469130905961279 1104 88-89 0.1309806600780945 1104 90-91 -0.009025310467933423 1201 1 0.23217122849220573 1201 2 0.2779637686991592 1201 3 0.2335769578249014 1201 4 0.04037336732138641 1201 5 0.38226807257630213 1201 6 0.15329400874285426 1201 7 0.13276675870494614 1201 8 0.2311183576966016 1201 9 0.13075346063254045 1201 10-11 0.18127785065025392 1201 12-13 0.11793829287234558 1201 14-15 0.05523895615160512 1201 16-17 0.04811471409087176 1201 18-19 0.0028174149944177884 1201 20-21 0.006765391849725688 1201 22-23 0.0012611545939051894 1201 24-25 0.005871108456517504 1201 26-27 0.07384551103537262 1201 28-29 0.005623463656100114 1201 30-31 -0.006838769301303671 1201 32-33 0.0907144979188601 1201 34-35 -0.05122955660154105 1201 36-37 -0.012618111274242949 1201 38-39 0.001011665681126317 1201 40-41 0.009420999969528054 1201 42-43 0.0734211257138 1201 44-45 -0.008064460733123724 1201 46-47 0.05997948954581034 1201 48-49 -0.03454590456971118 1201 50-51 -0.09276132537749504 1201 52-53 0.047689457091820486 1201 54-55 0.13176166108495124 1201 56-57 0.007230657483745517 1201 58-59 0.03202909092367179 1201 60-61 -0.006950457764780538 1201 62-63 -0.10587210640406397 1201 64-65 -0.18426065216218035 1201 66-67 6.913707912588052E-4 1201 68-69 0.08673022671339226 1201 70-71 -0.041518747550448154 1201 72-73 0.09535689480851062 1201 74-75 -0.11407388961233522 1201 76-77 -0.046844647274177476 1201 78-79 0.05545338849857018 1201 80-81 0.06149619381573501 1201 82-83 0.12904354458726885 1201 84-85 -0.41585337797500443 1201 86-87 -0.5827165390334983 1201 88-89 -0.9014568941862464 1201 90-91 -0.7074380088806294 1202 1 -0.0014408059562001085 1202 2 0.17694616528465446 1202 3 0.16659977086675326 1202 4 0.13871292936747182 1202 5 0.11744273370207736 1202 6 0.013130972513451411 1202 7 0.08004281926424994 1202 8 0.025189006729341656 1202 9 0.03693570867043405 1202 10-11 0.06590238872734488 1202 12-13 0.07667710276540873 1202 14-15 0.014851267819132374 1202 16-17 -0.059123320373217325 1202 18-19 -0.015088063744705948 1202 20-21 0.012997591635802053 1202 22-23 -0.08430932836888871 1202 24-25 -0.024489616393033486 1202 26-27 -0.010146653601402988 1202 28-29 -0.009912576137921292 1202 30-31 -0.058130845803319176 1202 32-33 -0.0757721327925438 1202 34-35 -0.053739035543515 1202 36-37 0.046669885546457124 1202 38-39 -0.038109808699715586 1202 40-41 -0.12990133783790014 1202 42-43 0.13205115202705997 1202 44-45 -0.009048893375819489 1202 46-47 0.07470051329351435 1202 48-49 -0.05017161602232534 1202 50-51 0.06994043487493684 1202 52-53 -0.08298551647540364 1202 54-55 -0.04562222022862272 1202 56-57 0.06607599496105365 1202 58-59 -0.0031822548740407797 1202 60-61 -0.2176627347204061 1202 62-63 -0.11330948122166262 1202 64-65 -0.025491127322773366 1202 66-67 0.030078815296604944 1202 68-69 0.042455488308675626 1202 70-71 0.14047728190636377 1202 72-73 0.19223592710938675 1202 74-75 -0.10348675313882438 1202 76-77 0.15507331982842487 1202 78-79 0.20668707080249504 1202 80-81 0.2824775821390162 1202 82-83 -0.16511291067173062 1202 84-85 0.021802722617849213 1202 86-87 -0.3017641580811201 1202 88-89 -1.337145299983355 1202 90-91 -0.17996548140810376 1203 1 0.3289764708341565 1203 2 0.05454701339110812 1203 3 0.08470496947117567 1203 4 0.1385395341813549 1203 5 -0.061308578714616146 1203 6 0.14787560666554356 1203 7 0.0579285582114224 1203 8 0.03213373892621263 1203 9 0.039628433913648564 1203 10-11 0.013842396542877111 1203 12-13 -0.02125165348749647 1203 14-15 -0.04311946026853519 1203 16-17 0.04360886502448125 1203 18-19 0.1366958348548053 1203 20-21 0.039503468266403274 1203 22-23 0.08716066119552579 1203 24-25 0.07591065874432701 1203 26-27 0.04957906326337991 1203 28-29 0.01600936670413944 1203 30-31 0.0640260628155005 1203 32-33 -0.0101097833920889 1203 34-35 0.01101739062293916 1203 36-37 0.038361016734960174 1203 38-39 0.04349946519248249 1203 40-41 0.09414615796696779 1203 42-43 0.06557035963039226 1203 44-45 -0.002703749847498216 1203 46-47 -0.04557452954595931 1203 48-49 0.06735939295176507 1203 50-51 0.07724783861824847 1203 52-53 0.12312954533528142 1203 54-55 -0.020854069461989866 1203 56-57 -0.0734528922441271 1203 58-59 -0.07924545802664795 1203 60-61 -0.08391331547430525 1203 62-63 0.0696350283051288 1203 64-65 0.038651145001260545 1203 66-67 -0.15459408169554223 1203 68-69 0.007379151559042896 1203 70-71 0.09586494155737313 1203 72-73 -0.2601041617162636 1203 74-75 -0.030167096394386306 1203 76-77 -0.09591203279032356 1203 78-79 -0.06558865299997763 1203 80-81 -0.0701888619997959 1203 82-83 0.23476241042414614 1203 84-85 0.4668115409599949 1203 86-87 0.2467196451491631 1203 88-89 0.34422724903625834 1203 90-91 0.43541913397650944 1204 1 0.30137540705860744 1204 2 0.07504140885619393 1204 3 -0.04610963701869508 1204 4 -0.023527109682177638 1204 5 -0.014172210710320599 1204 6 -0.18955024065787995 1204 7 -0.15272297804942525 1204 8 -0.2431687038828727 1204 9 -0.22441581034934188 1204 10-11 -0.2013302167057489 1204 12-13 -0.17195133588121791 1204 14-15 -0.18044871870005608 1204 16-17 -0.12220268405308587 1204 18-19 -0.12680953855889499 1204 20-21 -0.09956723288676983 1204 22-23 -0.14638114470776742 1204 24-25 -0.09976432797701307 1204 26-27 -0.11285898126576654 1204 28-29 -0.1631651906817453 1204 30-31 -0.11951137094185071 1204 32-33 -0.14886849090033394 1204 34-35 -0.08187595427892802 1204 36-37 -0.191467661171437 1204 38-39 -0.04889219699761327 1204 40-41 -0.13320664481738476 1204 42-43 -0.18430623813363667 1204 44-45 -0.20550333641230623 1204 46-47 -0.11512646369952506 1204 48-49 -0.1139538002629763 1204 50-51 -0.021782141129094157 1204 52-53 -0.18882075560867406 1204 54-55 -0.1475639450877253 1204 56-57 -0.1514833319043447 1204 58-59 -0.22542125702671 1204 60-61 -0.14764741020428573 1204 62-63 -3.0189077286735255E-4 1204 64-65 -0.24130054703665849 1204 66-67 -0.1840182374954651 1204 68-69 0.07407657071547646 1204 70-71 0.020087886062007954 1204 72-73 0.10953535564116379 1204 74-75 -0.18946608146720934 1204 76-77 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0.17902946450342228 2401 54-55 0.14982693994620888 2401 56-57 0.09389882352191137 2401 58-59 0.14004523168041771 2401 60-61 0.14139690286328488 2401 62-63 0.12753240075637962 2401 64-65 0.12832457643784778 2401 66-67 0.0924085553266778 2401 68-69 -0.019607193191042427 2401 70-71 0.12800523394675878 2401 72-73 0.21145394450482513 2401 74-75 0.16541276348852563 2401 76-77 0.1498975485327989 2401 78-79 -0.06831581865163372 2401 80-81 -0.24073184851926044 2401 82-83 0.14297920394317742 2401 84-85 0.45915023239694364 2401 86-87 0.4655530420229681 2401 88-89 0.5795213666833163 2401 90-91 0.43541913397650944 2402 1 0.4476089647855943 2402 2 0.31192478934261203 2402 3 0.3221144991960969 2402 4 0.2969235070537479 2402 5 0.0798963921199416 2402 6 0.1919972043317344 2402 7 0.1620787875476708 2402 8 0.1360774799452642 2402 9 0.16317865362753992 2402 10-11 0.18850632132001266 2402 12-13 0.16440364441423583 2402 14-15 0.198349629772558 2402 16-17 0.1015726135225421 2402 18-19 0.09699804289633107 2402 20-21 0.06182815916887563 2402 22-23 0.04839784833733063 2402 24-25 0.07766787432370847 2402 26-27 0.06447636939613943 2402 28-29 0.130112332929464 2402 30-31 0.07641825268399316 2402 32-33 0.10293460558509082 2402 34-35 0.08625909437792956 2402 36-37 0.07138036730969333 2402 38-39 0.05506390871106959 2402 40-41 0.04351150728627573 2402 42-43 0.0796743461027134 2402 44-45 0.16557604590505548 2402 46-47 0.1196127190293339 2402 48-49 0.08723483283761624 2402 50-51 0.13670837948940573 2402 52-53 0.115401907837402 2402 54-55 0.13785402688311876 2402 56-57 0.11713833282581732 2402 58-59 0.1914778807096269 2402 60-61 0.16314876625386887 2402 62-63 0.3004621995578276 2402 64-65 0.1323636002169266 2402 66-67 0.20895231681660675 2402 68-69 0.17016412957823235 2402 70-71 0.18388716229557645 2402 72-73 0.12853811373920365 2402 74-75 -0.05853793206075153 2402 76-77 0.08056847499502595 2402 78-79 0.3137015639699712 2402 80-81 0.26907245550174963 2402 82-83 -0.26086606464649975 2402 84-85 -0.06139179095994507 2402 86-87 -0.03876049507744739 2402 88-89 -0.05153453393780438 2402 90-91 0.43541913397650944 2403 1 -0.22868469675209724 2403 2 -0.07434989102054601 2403 3 0.03250487611533259 2403 4 0.07684469547467643 2403 5 0.14572647658488336 2403 6 0.12094097251763003 2403 7 0.10819124522355139 2403 8 0.1384567893151356 2403 9 0.10712175691091375 2403 10-11 0.1323033219129428 2403 12-13 0.1509335174766946 2403 14-15 0.13040565582537056 2403 16-17 0.17358879412494588 2403 18-19 0.08869291662640677 2403 20-21 0.11177769286454975 2403 22-23 0.06492936776066927 2403 24-25 0.1072109205729177 2403 26-27 0.062156853905129594 2403 28-29 0.1005757801331626 2403 30-31 0.03440588292676239 2403 32-33 0.09782591135390817 2403 34-35 0.07906089566608898 2403 36-37 0.09854684080057297 2403 38-39 0.09489714821724249 2403 40-41 0.14185692062359578 2403 42-43 0.18020016874425693 2403 44-45 0.0378093015310057 2403 46-47 0.0677815605271519 2403 48-49 0.09037905166977822 2403 50-51 0.04996048793640995 2403 52-53 0.0716869867229164 2403 54-55 0.15171308233740888 2403 56-57 0.10325582298974467 2403 58-59 0.16156572838539773 2403 60-61 0.19122836712310232 2403 62-63 0.2800281954251176 2403 64-65 0.16381292391976388 2403 66-67 0.2598850298008557 2403 68-69 0.10345246432070354 2403 70-71 0.08236404819661658 2403 72-73 0.16597861108632372 2403 74-75 0.19757953542295326 2403 76-77 0.4280647007817393 2403 78-79 0.2121936878153221 2403 80-81 0.3562077691532366 2403 82-83 0.230512973966583 2403 84-85 0.3884277710111874 2403 86-87 0.12374713618017807 2403 88-89 0.5795213666833163 2403 90-91 -0.7286020300446552 2404 1 -0.45965142510127777 2404 2 -0.26560362755715516 2404 3 -0.32799248524399616 2404 4 -0.3269337536056227 2404 5 -0.2300755278330584 2404 6 -0.1997943673424487 2404 7 -0.14889776210981864 2404 8 0.09146631583598008 2404 9 -0.0037122222435570507 2404 10-11 0.06854469558768983 2404 12-13 0.036377763342123615 2404 14-15 0.10349916545469995 2404 16-17 0.011983520006822346 2404 18-19 0.009238464150932657 2404 20-21 -0.0025492560654925 2404 22-23 0.02900536904704154 2404 24-25 -0.006449382778498602 2404 26-27 0.027769118707617224 2404 28-29 0.031193765065758328 2404 30-31 -0.034680372417739136 2404 32-33 0.03341791317320286 2404 34-35 -0.04227859843209103 2404 36-37 -0.020106850798754294 2404 38-39 0.007303378370451696 2404 40-41 0.09835281344560798 2404 42-43 0.051057350344272834 2404 44-45 0.04937546190598141 2404 46-47 -0.01307765201349298 2404 48-49 -0.03674463835572794 2404 50-51 0.038639378367363975 2404 52-53 0.006856808499229317 2404 54-55 -0.10684274509748803 2404 56-57 -0.03379324648904003 2404 58-59 -0.01365408049403527 2404 60-61 -0.06220723270376993 2404 62-63 0.04273510427310612 2404 64-65 -0.09134128579026424 2404 66-67 0.10257363670276476 2404 68-69 0.0235451885462723 2404 70-71 -0.056050900090347966 2404 72-73 -0.10865955372715774 2404 74-75 -0.01920397417914188 2404 76-77 -0.035297408982479794 2404 78-79 0.1184929281571101 2404 80-81 0.14178182222971003 2404 82-83 -0.1496539434343802 2404 84-85 -0.24386873853748625 2404 86-87 -0.7749434849212875 2404 88-89 0.43406682122877527 2404 90-91 0.054466753024129844 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 12.0 31 373.0 32 2767.0 33 4986.0 34 6507.0 35 8038.0 36 11674.0 37 18097.0 38 47130.0 39 281051.0 40 298398.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.750744453051595 12.235538760157992 32.793193740409 30.220523046381416 2 26.28906215481412 18.28163338645207 29.17014000371144 26.259164455022372 3 27.11975482637708 20.165559939266217 23.197241944011225 29.51744329034548 4 26.304170784041425 21.05818126659529 22.201571941275315 30.43607600808797 5 27.100302930785396 21.216936437551638 22.10319675185153 29.579563879811438 6 27.90365122166375 23.142468775449796 24.33872875103272 24.61515125185374 7 26.2140426164855 22.911110358406734 23.770420583388436 27.104426441719326 8 25.06859018633851 23.715342258770928 24.022691091596432 27.19337646329413 9 25.334114836834143 23.97350349688454 24.304415249332507 26.38796641694881 10-11 24.797542976556368 22.915012966969208 24.351541088577434 27.935902967896997 12-13 25.241188572573055 22.91604384470269 24.006270681984528 27.836496900739725 14-15 25.734242665673097 22.447657183082413 23.832199613273584 27.98590053797091 16-17 25.64948978915605 22.19973108817981 23.519254587037743 28.631524535626397 18-19 25.97929702974671 22.522027648140813 23.251594546951328 28.247080775161148 20-21 26.07664134143701 22.672535797229294 23.0695709928678 28.18125186846589 22-23 25.74418327238882 22.249139585263162 23.400703647687227 28.60597349466079 24-25 25.90006671251618 22.678426527134913 23.429789126596205 27.991717633752707 26-27 25.928121313691676 22.77069008428162 23.375299874969258 27.925888727057448 28-29 25.805594131654868 22.403255806418834 23.549518211927843 28.241631849998456 30-31 26.120162407901848 22.554124922131685 23.572119682312 27.753592987654468 32-33 26.060971746639915 22.687538654156548 23.568928846182853 27.68256075302068 34-35 25.809590177845877 22.328616378025917 23.856562637115108 28.0052308070131 36-37 26.069193444591164 22.62355010966191 23.77049380893461 27.536762636812306 38-39 26.134920533114407 22.62614860328985 23.724104313916648 27.514826549679093 40-41 25.93555309116154 22.356621856770623 23.81145582573729 27.89636922633055 42-43 26.119947203274087 22.6480176855651 23.817975545672894 27.414059565487914 44-45 26.24983704862469 22.589338091513493 23.75494964802503 27.40587521183679 46-47 25.972220404851942 22.24537551958616 23.88079068692235 27.901613388639547 48-49 26.135271990231644 22.7280777996338 23.743972487211877 27.39267772292267 50-51 26.35505436705786 22.582400369338323 23.686483511047808 27.37606175255601 52-53 25.934736023431487 22.3553389650541 23.84945489245399 27.860470119060427 54-55 26.192486408413966 22.630631499914063 23.896013436296695 27.280868655375272 56-57 26.242093061765193 22.614008941877795 23.743831760225202 27.40006623613181 58-59 26.161671732578352 22.34770736272232 23.853407198773215 27.63721370592611 60-61 26.267399862018586 22.707479404244957 23.747412848504528 27.277707885231933 62-63 26.331636903683847 22.627639729054223 23.629197305031333 27.411526062230596 64-65 26.040091830449636 22.265829808245396 23.86700874018193 27.82706962112304 66-67 26.469502523431863 22.541888968997835 23.711896178803173 27.276712328767122 68-69 26.360680364890644 22.572419723070535 23.500130129833334 27.566769782205487 70-71 26.1375199331764 22.4003341180044 23.80400941605285 27.65813653276635 72-73 26.652245031850242 22.491544282250306 23.50179753265924 27.354413153240216 74-75 26.744423446391274 22.537600184271003 23.505022955350928 27.212953413986796 76-77 26.451883999799307 22.16835582760524 23.612086699111938 27.767673473483516 78-79 26.432190851456916 22.430565670348876 23.62568706064532 27.51155641754889 80-81 26.74683786891529 22.28440015331545 23.26945189727865 27.69931008049061 82-83 26.50027868953985 22.12175636341116 22.916950517123922 28.461014429925065 84-85 27.398517826145014 21.887101081586327 22.598143944451863 28.116237147816797 86-87 27.433671360132294 21.02818511222976 22.57484010602855 28.9633034216094 88-89 26.516168395363028 20.032540166768353 22.928615009151923 30.5226764287167 90-91 25.09065269943594 21.494762288477034 24.18412570507655 29.230459307010477 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 2.5 10 5.5 11 8.0 12 10.5 13 15.5 14 24.5 15 41.5 16 70.5 17 116.0 18 170.0 19 233.5 20 357.5 21 519.5 22 678.0 23 917.0 24 1259.5 25 1623.5 26 2041.0 27 2591.0 28 3208.5 29 3862.0 30 4586.5 31 5474.5 32 6522.5 33 7447.5 34 8536.0 35 9882.0 36 11322.0 37 12820.5 38 14457.5 39 16495.0 40 18662.5 41 20773.0 42 23072.5 43 25731.5 44 28562.0 45 31327.0 46 34316.5 47 37466.5 48 40200.0 49 42776.5 50 45381.5 51 47728.5 52 49314.5 53 50486.0 54 51423.5 55 51645.5 56 51258.5 57 50288.5 58 48993.5 59 47364.0 60 45395.5 61 42950.0 62 40446.0 63 38123.5 64 35369.0 65 32200.0 66 28835.5 67 26014.0 68 23298.0 69 20056.5 70 16892.5 71 14190.5 72 11705.5 73 9220.0 74 7092.5 75 5595.5 76 4370.5 77 3179.0 78 2337.0 79 1705.0 80 1172.5 81 821.0 82 577.5 83 399.0 84 283.0 85 191.0 86 112.5 87 68.5 88 51.0 89 37.5 90 23.0 91 16.0 92 10.0 93 6.5 94 5.5 95 4.0 96 2.0 97 1.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0016199507240443397 2 0.007510680629660119 3 5.89072990561578E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 10426.0 32-33 23099.0 34-35 25726.0 36-37 27721.0 38-39 29866.0 40-41 31108.0 42-43 31984.0 44-45 32546.0 46-47 32293.0 48-49 32002.0 50-51 31820.0 52-53 31340.0 54-55 29836.0 56-57 28769.0 58-59 27559.0 60-61 25869.0 62-63 24633.0 64-65 23503.0 66-67 21935.0 68-69 20471.0 70-71 19185.0 72-73 17710.0 74-75 16174.0 76-77 14819.0 78-79 13328.0 80-81 12186.0 82-83 10757.0 84-85 9610.0 86-87 8552.0 88-89 7542.0 90-91 6664.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.8202616321261 #Duplication Level Percentage of deduplicated Percentage of total 1 99.83352732572882 99.65408817312263 2 0.15784927422639983 0.315131117034409 3 0.005435209687093853 0.01627632158973524 4 0.002504092880872189 0.009998368260792251 5 0.0 0.0 6 4.7932309599291455E-4 0.002870769410900005 7 0.0 0.0 8 2.0477438082276838E-4 0.0016352505815428295 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001472682476403945 0.0 0.0 0.0 0.0 2 0.001472682476403945 0.0 0.0 0.0 1.472682476403945E-4 3 0.001472682476403945 0.0 0.0 0.0 1.472682476403945E-4 4 0.001472682476403945 0.0 0.0 0.0 1.472682476403945E-4 5 0.001472682476403945 0.0 0.0 0.0 1.472682476403945E-4 6 0.0016199507240443395 0.0 0.0 0.0 1.472682476403945E-4 7 0.002061755466965523 0.0 0.0 0.0 1.472682476403945E-4 8 0.005743461657975385 0.0 0.0 0.0 2.94536495280789E-4 9 0.00589072990561578 0.0 0.0 0.0 2.94536495280789E-4 10-11 0.00589072990561578 0.0 0.0 0.0 2.94536495280789E-4 12-13 0.00589072990561578 0.0 0.0 0.0 2.94536495280789E-4 14-15 0.00589072990561578 0.0 0.0 0.0 3.6817061910098626E-4 16-17 0.00589072990561578 0.0 0.0 0.0 5.154388667413807E-4 18-19 0.00589072990561578 0.0 0.0 0.0 7.363412382019725E-4 20-21 0.006258900524716766 0.0 0.0 0.0 8.83609485842367E-4 22-23 0.006627071143817753 0.0 0.0 0.0 8.83609485842367E-4 24-25 0.006921607639098541 0.0 0.0 0.0 8.83609485842367E-4 26-27 0.007216144134379331 0.0 0.0 0.0 9.572436096625642E-4 28-29 0.007584314753480317 0.0 0.0 0.0 0.0010308777334827614 30-31 0.007805217124940909 0.0 0.0 0.0 0.0010308777334827614 32-33 0.008247021867862091 0.0 0.0 0.0 0.0010308777334827614 34-35 0.008909728982243868 0.0 0.0 0.0 0.0010308777334827614 36-37 0.009498801972805445 0.0 0.0 0.0 0.0010308777334827614 38-39 0.010087874963367023 0.0 0.0 0.0 0.0010308777334827614 40-41 0.010529679706288207 0.0 0.0 0.0 0.0010308777334827614 42-43 0.010603313830108404 2.94536495280789E-4 0.0 0.0 0.0010308777334827614 44-45 0.010750582077748799 2.94536495280789E-4 0.0 0.0 0.0010308777334827614 46-47 0.010897850325389194 2.94536495280789E-4 0.0 0.0 0.0011045118573029587 48-49 0.011045118573029589 2.94536495280789E-4 0.0 0.0 0.001178145981123156 50-51 0.011192386820669982 2.94536495280789E-4 0.0 0.0 0.001178145981123156 52-53 0.011339655068310377 2.94536495280789E-4 0.0 0.0 0.001178145981123156 54-55 0.01148692331595077 2.94536495280789E-4 0.0 0.0 0.001178145981123156 56-57 0.011560557439770969 2.94536495280789E-4 0.0 0.0 0.001178145981123156 58-59 0.012149630430332547 2.94536495280789E-4 0.0 0.0 0.001178145981123156 60-61 0.012370532801793139 2.94536495280789E-4 0.0 0.0 0.001178145981123156 62-63 0.012444166925613336 2.94536495280789E-4 0.0 0.0 0.001178145981123156 64-65 0.012517801049433532 2.94536495280789E-4 0.0 0.0 0.001178145981123156 66-67 0.012517801049433532 2.94536495280789E-4 0.0 0.0 0.001178145981123156 68-69 0.012591435173253729 2.94536495280789E-4 0.0 0.0 0.001178145981123156 70-71 0.012665069297073927 2.94536495280789E-4 0.0 0.0 0.001178145981123156 72-73 0.012665069297073927 2.94536495280789E-4 0.0 0.0 0.001178145981123156 74-75 0.012665069297073927 2.94536495280789E-4 0.0 0.0 0.001178145981123156 76-77 0.012738703420894126 2.94536495280789E-4 0.0 0.0 0.001178145981123156 78-79 0.012812337544714322 2.94536495280789E-4 0.0 0.0 0.001178145981123156 80 0.012812337544714322 2.94536495280789E-4 0.0 0.0 0.001178145981123156 >>END_MODULE