##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-7A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 662499 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.294383840579385 40.0 40.0 40.0 40.0 40.0 2 39.1689104436384 40.0 40.0 40.0 40.0 40.0 3 39.15846514485305 40.0 40.0 40.0 40.0 40.0 4 39.07102652230419 40.0 40.0 40.0 40.0 40.0 5 39.2081437104056 40.0 40.0 40.0 40.0 40.0 6 39.268919651199475 40.0 40.0 40.0 40.0 40.0 7 39.28807741596591 40.0 40.0 40.0 40.0 40.0 8 39.30862386207375 40.0 40.0 40.0 40.0 40.0 9 39.31489104134497 40.0 40.0 40.0 40.0 40.0 10-11 39.314661607036385 40.0 40.0 40.0 40.0 40.0 12-13 39.31044424217998 40.0 40.0 40.0 40.0 40.0 14-15 39.32216350515246 40.0 40.0 40.0 40.0 40.0 16-17 39.459540316287274 40.0 40.0 40.0 40.0 40.0 18-19 39.475123736035826 40.0 40.0 40.0 40.0 40.0 20-21 39.474082225029775 40.0 40.0 40.0 40.0 40.0 22-23 39.48294261576244 40.0 40.0 40.0 40.0 40.0 24-25 39.47824826905399 40.0 40.0 40.0 40.0 40.0 26-27 39.47550109509599 40.0 40.0 40.0 40.0 40.0 28-29 39.47460147109656 40.0 40.0 40.0 40.0 40.0 30-31 39.473222460219525 40.0 40.0 40.0 40.0 40.0 32-33 39.47627749772252 40.0 40.0 40.0 40.0 40.0 34-35 39.47686075946703 40.0 40.0 40.0 40.0 40.0 36-37 39.475839734325426 40.0 40.0 40.0 40.0 40.0 38-39 39.46271873156809 40.0 40.0 40.0 40.0 40.0 40-41 39.454742255776566 40.0 40.0 40.0 40.0 40.0 42-43 39.45157985687874 40.0 40.0 40.0 40.0 40.0 44-45 39.44254446261576 40.0 40.0 40.0 40.0 40.0 46-47 39.4340800647778 40.0 40.0 40.0 40.0 40.0 48-49 39.44227474150637 40.0 40.0 40.0 40.0 40.0 50-51 39.43814262510139 40.0 40.0 40.0 40.0 40.0 52-53 39.421526376070815 40.0 40.0 40.0 40.0 40.0 54-55 39.416571782669806 40.0 40.0 40.0 40.0 40.0 56-57 39.41198144217931 40.0 40.0 40.0 40.0 40.0 58-59 39.40914452556058 40.0 40.0 40.0 40.0 40.0 60-61 39.37289733248501 40.0 40.0 40.0 40.0 40.0 62-63 39.39139565733082 40.0 40.0 40.0 40.0 40.0 64-65 39.38636319712711 40.0 40.0 40.0 40.0 40.0 66-67 39.378576104938986 40.0 40.0 40.0 40.0 40.0 68-69 39.37820702661177 40.0 40.0 40.0 40.0 40.0 70-71 39.37181998035399 40.0 40.0 40.0 40.0 40.0 72-73 39.35689166912837 40.0 40.0 40.0 40.0 40.0 74-75 39.36053551807086 40.0 40.0 40.0 40.0 40.0 76-77 39.36366408240397 40.0 40.0 40.0 40.0 40.0 78-79 39.334167936140446 40.0 40.0 40.0 40.0 40.0 80-81 39.32569702361957 40.0 40.0 40.0 40.0 40.0 82-83 39.32955906163038 40.0 40.0 40.0 40.0 40.0 84-85 39.32248845833247 40.0 40.0 40.0 40.0 40.0 86-87 39.34926170744397 40.0 40.0 40.0 40.0 40.0 88-89 39.32216324357156 40.0 40.0 40.0 40.0 40.0 90-91 39.48892387827654 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1942895196510932 1101 2 0.35013663418367713 1101 3 0.22775943181132163 1101 4 0.11542940316777361 1101 5 0.18570423466319141 1101 6 0.10641389158937642 1101 7 0.09920727779316252 1101 8 -0.11526072809773069 1101 9 0.03916773801453388 1101 10-11 -0.019595281035073242 1101 12-13 0.13058196491329 1101 14-15 0.045203863993521054 1101 16-17 0.0025401044720680943 1101 18-19 -0.07085127569967398 1101 20-21 0.03206789156193679 1101 22-23 0.10746420600802509 1101 24-25 -0.03651827859662404 1101 26-27 -0.002197978282715951 1101 28-29 0.035784927192331395 1101 30-31 0.04430759198648815 1101 32-33 -2.4717276509278463E-4 1101 34-35 -0.07980373494965676 1101 36-37 0.056497326813399695 1101 38-39 -0.012675633962651034 1101 40-41 0.032809717998588894 1101 42-43 0.08831908844589265 1101 44-45 0.07694834294984787 1101 46-47 0.06691525336346871 1101 48-49 0.09304926307130756 1101 50-51 0.001819829030345943 1101 52-53 0.02123435398318918 1101 54-55 0.08332026931704206 1101 56-57 0.1087903224600737 1101 58-59 0.1569816853447108 1101 60-61 -0.02476218797558971 1101 62-63 -0.0086992083611932 1101 64-65 0.015553114418061398 1101 66-67 0.04026187157304406 1101 68-69 0.07147175046902277 1101 70-71 -0.030442004323631977 1101 72-73 0.14263676713616036 1101 74-75 -0.059240559342519816 1101 76-77 0.1323893419281319 1101 78-79 -0.1171262525540584 1101 80-81 -0.04595776879978786 1101 82-83 -0.1298574826774228 1101 84-85 -0.36574132836873474 1101 86-87 0.41599536245848867 1101 88-89 0.1296299442273039 1101 90-91 0.031817963433354635 1102 1 0.31853164598143024 1102 2 0.17491990150990944 1102 3 0.28524767934260353 1102 4 -0.13190956235664686 1102 5 0.2038996611211914 1102 6 0.07037896750770756 1102 7 0.08194616452527015 1102 8 0.058363653601496424 1102 9 -0.0557461394067289 1102 10-11 -0.03522385781487003 1102 12-13 -0.11067104810322803 1102 14-15 0.044082257606319786 1102 16-17 0.0378538421051573 1102 18-19 0.006958642515627389 1102 20-21 0.05120624239151539 1102 22-23 0.08974670012192121 1102 24-25 0.022845771299429884 1102 26-27 -0.09058242735008548 1102 28-29 0.03220554609229964 1102 30-31 -0.008041215400751867 1102 32-33 -0.026108423504950906 1102 34-35 0.0025188540945251248 1102 36-37 -0.019743241586446914 1102 38-39 0.018552071883156884 1102 40-41 -0.05236797924106895 1102 42-43 0.11004441377502161 1102 44-45 0.015885372445012536 1102 46-47 -0.1262138243790858 1102 48-49 -0.0016830673725891643 1102 50-51 0.022507381743160693 1102 52-53 0.04890001667772026 1102 54-55 -0.11482743717377275 1102 56-57 -0.008544466617344426 1102 58-59 -0.049171361056913554 1102 60-61 -0.151850740210989 1102 62-63 -0.21610546071593717 1102 64-65 0.03532610501230948 1102 66-67 -0.16073985758448117 1102 68-69 -0.0999741778830412 1102 70-71 -0.14567336930444696 1102 72-73 -0.08944033473696322 1102 74-75 -0.11003588917119345 1102 76-77 -0.13529598995603465 1102 78-79 -0.2705756663428147 1102 80-81 -0.055992923801383654 1102 82-83 0.12737668492673038 1102 84-85 -0.16095593331013447 1102 86-87 0.07907334727560311 1102 88-89 0.7210277936896716 1102 90-91 -3.154995223379835 1103 1 0.2567120438799648 1103 2 0.01831260124069445 1103 3 0.10640054113795117 1103 4 -0.08614336185662097 1103 5 0.11901213817203171 1103 6 0.09053307612013128 1103 7 0.14171685703216497 1103 8 -0.04726709361518289 1103 9 -0.049807333795406805 1103 10-11 0.0696062407970146 1103 12-13 0.06641379682434945 1103 14-15 0.05707673392509349 1103 16-17 -7.13740506398608E-4 1103 18-19 -0.07810720343206867 1103 20-21 0.12018186700932887 1103 22-23 -0.04437529735556467 1103 24-25 0.022048732471944277 1103 26-27 -0.004222837218641473 1103 28-29 0.06809106417114208 1103 30-31 0.018333261452269767 1103 32-33 0.09746120659103497 1103 34-35 0.005288600961080192 1103 36-37 -0.06246550825689212 1103 38-39 0.08434375659229687 1103 40-41 0.02860306653828104 1103 42-43 -0.08587220002244322 1103 44-45 -0.058094195149024586 1103 46-47 -0.002925788198062662 1103 48-49 0.009503904762240722 1103 50-51 -0.09681519927722348 1103 52-53 -0.142900724840203 1103 54-55 -0.0662318527544059 1103 56-57 -0.07146782124370077 1103 58-59 0.1629251058820529 1103 60-61 0.09073582412654702 1103 62-63 -0.18025521469605366 1103 64-65 -0.17995372813846444 1103 66-67 0.011965111122378858 1103 68-69 -0.037260323583126365 1103 70-71 -0.08314846053094982 1103 72-73 -0.10112149801283721 1103 74-75 -0.23121488938244994 1103 76-77 -0.3980754629468919 1103 78-79 -0.4073574845750372 1103 80-81 -0.3047843223631901 1103 82-83 0.04440373732900582 1103 84-85 -0.2814826102701602 1103 86-87 -0.49821603001377923 1103 88-89 -0.39008331742144264 1103 90-91 0.10324653486192403 1104 1 0.29125991983617894 1104 2 0.22711171804050423 1104 3 0.18485360262101125 1104 4 0.12196673351732557 1104 5 0.1813813895943852 1104 6 0.18505571168402923 1104 7 0.1351597647025855 1104 8 0.14691451905085273 1104 9 0.12360825502963735 1104 10-11 -0.04965568059047598 1104 12-13 -0.035973505820486196 1104 14-15 0.04613140079960942 1104 16-17 0.1093745154508241 1104 18-19 0.0646455083441495 1104 20-21 -0.05005767470600375 1104 22-23 0.010154911749623352 1104 24-25 0.001668503685515077 1104 26-27 -0.013838529823971157 1104 28-29 0.04720332270223082 1104 30-31 0.06389765043333284 1104 32-33 0.04512563453645413 1104 34-35 0.023926282183900582 1104 36-37 0.07089087137165961 1104 38-39 -0.015197261233957704 1104 40-41 0.08829260370528402 1104 42-43 -0.020759085618799133 1104 44-45 0.024144907217475975 1104 46-47 0.04237201839769256 1104 48-49 0.03814457497914958 1104 50-51 -0.041974937916357646 1104 52-53 -0.07730280465749928 1104 54-55 0.0706520286560206 1104 56-57 -0.020757464563736505 1104 58-59 0.010930429198545255 1104 60-61 -0.01444182786044479 1104 62-63 -0.009974979844500353 1104 64-65 -0.05001334450065542 1104 66-67 -0.06817093277913955 1104 68-69 -0.039359177577772186 1104 70-71 0.054184417784824745 1104 72-73 0.06346704388879232 1104 74-75 0.010204886662940282 1104 76-77 -0.06568236101112745 1104 78-79 0.03917222539076448 1104 80-81 0.34572292288090267 1104 82-83 0.22470558854327294 1104 84-85 0.33429479514478544 1104 86-87 -0.02360398680305309 1104 88-89 -0.10488313748442124 1104 90-91 0.603246534861924 1201 1 0.18844428782674072 1201 2 0.19577451307198146 1201 3 0.10805427484891794 1201 4 0.11446380268497336 1201 5 0.23347634178624332 1201 6 -0.06183082952720298 1201 7 0.03892003955706258 1201 8 -0.019014639512263898 1201 9 -0.032649495581942745 1201 10-11 -0.017444308283280918 1201 12-13 0.01584225494227809 1201 14-15 0.10245894926845267 1201 16-17 -0.041898448383051345 1201 18-19 -0.02377685678673913 1201 20-21 0.001777060231553662 1201 22-23 -0.04873143971985883 1201 24-25 -0.008516562283631401 1201 26-27 0.04921536360892276 1201 28-29 -0.006423116455039235 1201 30-31 0.07141277894948672 1201 32-33 0.04834228403144891 1201 34-35 -0.01853562580235746 1201 36-37 -0.01797777127411848 1201 38-39 -0.003912613907992579 1201 40-41 -0.03812279895235804 1201 42-43 -0.028693765945696725 1201 44-45 -0.09223408098873165 1201 46-47 -0.009899875860661211 1201 48-49 0.10862212842678787 1201 50-51 -0.015216947637618716 1201 52-53 2.1706948125199688E-4 1201 54-55 0.09769971527024524 1201 56-57 0.044996603122598344 1201 58-59 -0.04554736455276753 1201 60-61 0.02228993121115508 1201 62-63 -0.15237441962423048 1201 64-65 -0.158565228985303 1201 66-67 -0.15018023101130495 1201 68-69 0.07453890809330233 1201 70-71 -0.1933948053916481 1201 72-73 -0.22958459129075237 1201 74-75 -0.039046982244791195 1201 76-77 -0.6346289038473003 1201 78-79 -0.4700494638899144 1201 80-81 -0.4467770212319522 1201 82-83 -0.4916141470656612 1201 84-85 -0.8945980847260913 1201 86-87 0.21194453233043475 1201 88-89 0.16102779368966935 1201 90-91 -0.5396106079952148 1202 1 -0.07988203902559832 1202 2 0.10610031443824397 1202 3 -0.01397672882948342 1202 4 0.255372893594064 1202 5 0.11080161766643215 1202 6 0.13562056301089598 1202 7 -0.018029513779779904 1202 8 0.004369490808286969 1202 9 0.14108933783897015 1202 10-11 0.0173231641286975 1202 12-13 -0.0770571144744352 1202 14-15 0.005651277171764946 1202 16-17 0.030493826971657256 1202 18-19 0.035563658189616376 1202 20-21 0.0037870444669678704 1202 22-23 -0.0027514081906900856 1202 24-25 -0.02909591068089412 1202 26-27 -0.10620371153558494 1202 28-29 -0.06344492412713976 1202 30-31 -0.052720169027395514 1202 32-33 -0.07063072529525982 1202 34-35 -0.044351623709353305 1202 36-37 0.04704710112645216 1202 38-39 -0.12239152130673148 1202 40-41 -0.030116248083871255 1202 42-43 0.01989274929140805 1202 44-45 0.029363007446391975 1202 46-47 -0.10232168346018256 1202 48-49 -0.05579929677420381 1202 50-51 0.04439645877543086 1202 52-53 0.05868883876462405 1202 54-55 -0.005544198677171153 1202 56-57 0.017262900696863426 1202 58-59 0.029420959138043656 1202 60-61 -0.11161491275077395 1202 62-63 -0.18764573796514838 1202 64-65 -0.09666803110619782 1202 66-67 -0.10279130715790075 1202 68-69 0.17810756861891974 1202 70-71 0.07161392211584428 1202 72-73 3.5619406511244733E-4 1202 74-75 0.0429276486947856 1202 76-77 -0.0018641823719320882 1202 78-79 0.34675183618244176 1202 80-81 0.031502548660526486 1202 82-83 0.28904223231729986 1202 84-85 0.18146354822954436 1202 86-87 -0.13435766615540956 1202 88-89 -0.6804873578254842 1202 90-91 0.10324653486192403 1203 1 0.30310147887293937 1203 2 0.17832539813641546 1203 3 0.13568204016355878 1203 4 0.1416592701070698 1203 5 0.0809406853975645 1203 6 0.1282164939920989 1203 7 0.0859815601321472 1203 8 0.0856211003885079 1203 9 0.09052920392189634 1203 10-11 0.06341832251139579 1203 12-13 0.07471664292963709 1203 14-15 0.004189778330669469 1203 16-17 0.08292429310608895 1203 18-19 0.09927084335572545 1203 20-21 0.030177721778322564 1203 22-23 0.023645707441552588 1203 24-25 0.08350066413239432 1203 26-27 0.051976175149974324 1203 28-29 -0.011948014521166783 1203 30-31 -0.004632350330510349 1203 32-33 -0.02068718995009533 1203 34-35 0.004035392315557829 1203 36-37 0.024471416399727275 1203 38-39 -0.09272185178478054 1203 40-41 0.059524072550452445 1203 42-43 -0.020450736264187697 1203 44-45 0.08952768268989075 1203 46-47 0.07726081887908975 1203 48-49 -0.006997852375505431 1203 50-51 0.11603469306543701 1203 52-53 0.0861897396433875 1203 54-55 0.1334146739292521 1203 56-57 0.022634740019064736 1203 58-59 0.06343871304606097 1203 60-61 0.02855103468198905 1203 62-63 0.15265184399541454 1203 64-65 0.020234762591037736 1203 66-67 0.09759091261658881 1203 68-69 -0.1228571733024495 1203 70-71 -0.003210023791915262 1203 72-73 -0.06804422471606841 1203 74-75 0.1392130295692482 1203 76-77 0.046921076132008466 1203 78-79 0.2696828152531623 1203 80-81 0.05844893560691844 1203 82-83 -0.21260117816858326 1203 84-85 0.037587065979138856 1203 86-87 -0.48219380934039435 1203 88-89 -1.052918566463589 1203 90-91 0.603246534861924 1204 1 0.2101836318474426 1204 2 0.13753219700327435 1204 3 -0.0025615858987606543 1204 4 0.17112102648100347 1204 5 -0.009710872676087945 1204 6 -0.10067869014388009 1204 7 0.017992891576902537 1204 8 -0.09114943785512253 1204 9 0.09959435972926656 1204 10-11 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0.13976406405220132 2401 50-51 0.16081879646945652 2401 52-53 0.1441080670895971 2401 54-55 0.13405258283837895 2401 56-57 0.03068415173814998 2401 58-59 0.081401923213555 2401 60-61 0.10013367010486007 2401 62-63 0.09228043314411849 2401 64-65 0.1447834940621746 2401 66-67 0.17409130486928603 2401 68-69 0.341492884315997 2401 70-71 0.16471022718493344 2401 72-73 0.18758348091164123 2401 74-75 0.23620138180370276 2401 76-77 0.1233002525111786 2401 78-79 0.22662106843583985 2401 80-81 -0.16142502285499205 2401 82-83 0.14368090875571227 2401 84-85 -0.2595047063393068 2401 86-87 0.2059384679623122 2401 88-89 0.13769446035634303 2401 90-91 0.13657986819525547 2402 1 0.37244374802781977 2402 2 0.309726066118472 2402 3 0.30506288914009616 2402 4 0.15959998945540121 2402 5 0.14265517267173777 2402 6 -0.0250975126877222 2402 7 0.07531148374410179 2402 8 0.13210206951362835 2402 9 -0.022591242281293944 2402 10-11 0.09903080473711867 2402 12-13 0.1512193269829467 2402 14-15 0.12943090221598652 2402 16-17 0.0590709405195895 2402 18-19 0.040153329218973965 2402 20-21 0.054326485570612704 2402 22-23 0.03916581855143875 2402 24-25 0.01788410346838276 2402 26-27 0.032463603639513394 2402 28-29 0.01924432242397245 2402 30-31 0.08726037571969414 2402 32-33 0.02896061499696856 2402 34-35 0.04467731844391665 2402 36-37 0.035803814483358565 2402 38-39 0.07493703275384433 2402 40-41 0.026022417075317605 2402 42-43 0.03074310008259573 2402 44-45 0.09554572598591449 2402 46-47 0.12277635527387787 2402 48-49 0.14201407044140524 2402 50-51 0.040387133644479434 2402 52-53 0.16058313648549927 2402 54-55 0.07792272976764991 2402 56-57 0.029023168239966424 2402 58-59 -0.004515058190023069 2402 60-61 0.11496266457186977 2402 62-63 0.15347377549650076 2402 64-65 0.17577264038383333 2402 66-67 0.0966625805705803 2402 68-69 0.1377014608375262 2402 70-71 0.19592450717852472 2402 72-73 -0.02601845196024044 2402 74-75 0.20137110958437887 2402 76-77 0.3404199020417309 2402 78-79 -0.20974829088813607 2402 80-81 -0.29583153669386064 2402 82-83 -0.11899595775677341 2402 84-85 0.3578350324957569 2402 86-87 0.4170321574938072 2402 88-89 0.5048115774734541 2402 90-91 0.603246534861924 2403 1 -0.17146433530626837 2403 2 -0.19612495449236178 2403 3 0.03907993994643988 2403 4 -0.1930381308030391 2403 5 0.0691964710728854 2403 6 0.13111026279293725 2403 7 -0.05850093331152095 2403 8 0.2329780825259249 2403 9 0.18137443943497544 2403 10-11 0.08489241815387061 2403 12-13 0.13604753995853258 2403 14-15 0.0578783038187467 2403 16-17 0.08755896794050955 2403 18-19 0.11739414402241266 2403 20-21 0.08427463498936305 2403 22-23 0.0993437897281666 2403 24-25 0.1810680842568786 2403 26-27 0.10824911135691906 2403 28-29 0.08882340885465112 2403 30-31 0.110833581939346 2403 32-33 0.0932404725932372 2403 34-35 0.07129584101564745 2403 36-37 0.09972013367580246 2403 38-39 0.05050611972094288 2403 40-41 0.05775116728285923 2403 42-43 0.024881532856277033 2403 44-45 -0.010910630251856901 2403 46-47 -0.015053429049167733 2403 48-49 -0.032988856049605886 2403 50-51 0.12068681710889706 2403 52-53 0.08881040135055684 2403 54-55 0.01652748070313237 2403 56-57 0.015076856253550375 2403 58-59 -0.11405743145369485 2403 60-61 0.06799289292941069 2403 62-63 0.1676761686474464 2403 64-65 -0.00855683532333984 2403 66-67 0.1398466032026775 2403 68-69 0.16703097784223786 2403 70-71 0.09452212306718621 2403 72-73 0.2462555106363027 2403 74-75 0.14730558484712475 2403 76-77 0.28994513915606746 2403 78-79 0.138456620143522 2403 80-81 -0.0534554143469137 2403 82-83 0.2867668957793512 2403 84-85 -0.11889357575365977 2403 86-87 0.4339012409798997 2403 88-89 0.09880557146745161 2403 90-91 -0.012138080522689165 2404 1 -0.6128207772579941 2404 2 -0.4318299932756773 2404 3 -0.590922773511231 2404 4 -0.4116567537292468 2404 5 -0.28884008529830396 2404 6 -0.20073604792067812 2404 7 -0.21093031467903245 2404 8 -0.021694862149423955 2404 9 -0.14125926410005007 2404 10-11 0.03435456781002699 2404 12-13 0.13880104205219368 2404 14-15 0.04059226354242895 2404 16-17 0.08945300921136834 2404 18-19 -0.018303842161657258 2404 20-21 -0.006578980543572754 2404 22-23 0.04325112096485384 2404 24-25 0.00667583303134478 2404 26-27 0.021249920612795847 2404 28-29 0.037375295066340186 2404 30-31 -0.11605092636897041 2404 32-33 0.03482838017691847 2404 34-35 0.032118961951304925 2404 36-37 -0.02372880546245426 2404 38-39 0.06343296397518117 2404 40-41 7.901838793173965E-4 2404 42-43 0.10588897591501478 2404 44-45 0.018519133709560265 2404 46-47 -0.04731181324947187 2404 48-49 -0.004442882070534893 2404 50-51 0.04598845941689689 2404 52-53 0.11872143463580187 2404 54-55 -0.013294559605697032 2404 56-57 0.10142983151927609 2404 58-59 0.05758063172246608 2404 60-61 0.08316695238561067 2404 62-63 0.1048611256546721 2404 64-65 0.28357124097467334 2404 66-67 0.11300045201782893 2404 68-69 0.23164890331381827 2404 70-71 0.05924667485217583 2404 72-73 0.08052170964764827 2404 74-75 0.15425147761262537 2404 76-77 0.21177844098936305 2404 78-79 0.036963132335941395 2404 80-81 0.059166372251127086 2404 82-83 0.2748011694645669 2404 84-85 0.21493429542026377 2404 86-87 0.3987485041600607 2404 88-89 -0.4558952832334029 2404 90-91 0.20324653486192545 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 18.0 31 367.0 32 2407.0 33 4614.0 34 6386.0 35 7940.0 36 11580.0 37 17833.0 38 47090.0 39 276469.0 40 287795.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 25.060604688372656 12.40924391311567 32.23761868103669 30.29253271747498 2 26.355203671137346 18.7715577426732 28.7429524578657 26.13028612832376 3 26.73342500781145 20.483509914881605 22.958871769946366 29.824193307360574 4 26.037473264110588 21.48622111127715 22.063278586080884 30.413027038531382 5 26.85332355218649 21.636711904470797 21.9686369337916 29.54132760955111 6 27.462381075292193 23.617394139462853 24.20592332969559 24.714301455549368 7 25.734831297858562 23.322903128910383 23.974375810378582 26.967889762852472 8 24.82252803400458 24.226300718944483 24.215130890763607 26.736040356287333 9 24.934679146685504 24.53573514827947 24.492565271796636 26.03702043323839 10-11 24.44177274229848 23.330601253737743 24.646980599215997 27.580645404747784 12-13 24.97996223390526 23.293318178593477 24.281244198104453 27.44547538939681 14-15 25.32479294308369 22.998374337168812 24.04894196066711 27.627890759080394 16-17 25.323660865903193 22.678977628645477 23.839734097711844 28.157627407739483 18-19 25.59000089056738 22.925694982181106 23.715733910541754 27.768570216709758 20-21 25.667736856961294 23.07135557940465 23.583884654920233 27.677022908713823 22-23 25.38607605445442 22.645770031350988 23.840715231268273 28.127438682926314 24-25 25.518830971820332 22.997695090860514 23.886753036608358 27.596720900710793 26-27 25.696567089157867 23.065317834442016 23.789922701770116 27.448192374629997 28-29 25.541925346302406 22.672109693750482 23.919205915782516 27.866759044164596 30-31 25.71094768874076 22.935459232961406 23.940974737960683 27.412618340337147 32-33 25.757762229967746 22.934309857585053 23.900384618659384 27.407543293787818 34-35 25.50734931375873 22.592049210754247 24.138964459435357 27.761637016051665 36-37 25.725661831437435 22.946984705868537 24.11463636805897 27.212717094635057 38-39 25.725105559465167 22.925404644616467 24.04741379310345 27.302076002814918 40-41 25.528281097531032 22.605788272405206 24.187921005492473 27.678009624571292 42-43 25.7802501511428 22.987936056782583 24.17862723082842 27.0531865612462 44-45 25.814966469592832 22.816449746171685 24.134999059895126 27.23358472434036 46-47 25.513748530352615 22.664670290959478 24.265455442747673 27.556125735940235 48-49 25.841487467667395 22.922315374242373 24.099366065044318 27.13683109304591 50-51 25.93508200542602 22.855070868486997 24.01564351435176 27.194203611735222 52-53 25.68103884422958 22.628169093259878 24.176176288717745 27.514615773792794 54-55 25.84760691013697 22.931569547115824 24.162655972844718 27.058167569902487 56-57 25.971590060715467 22.76318283928269 24.088722760666307 27.176504339335533 58-59 25.71003676266834 22.619755777650514 24.250244776560567 27.41996268312058 60-61 26.087437153010924 22.814480601365847 23.989535055108828 27.108547190514404 62-63 26.189494027745177 22.675652207603374 23.976079073975736 27.158774690675713 64-65 25.905022751718835 22.578238574958036 23.997250887202064 27.519487786121065 66-67 26.21193771626298 22.740484429065745 23.972318339100347 27.07525951557093 68-69 26.41731689230426 22.64885190605251 23.771680568042367 27.162150633600863 70-71 26.152100228855385 22.31434248377841 23.98721124445299 27.54634604291321 72-73 26.17091730444508 22.653450582224075 23.84114383456503 27.334488278765818 74-75 26.375350255693107 22.682636090140203 23.64090470464995 27.30110894951674 76-77 26.166461159062887 22.22194821208385 23.837854500616523 27.77373612823674 78-79 26.292247638028304 22.674179022844257 23.577759629576907 27.45581370955053 80-81 26.375105130361646 22.50817680590599 23.362302588543127 27.754415475189237 82-83 26.521964197575453 22.09060156259405 23.469006946200054 27.918427293630444 84-85 27.167130964599316 21.8008243324699 22.939576104133067 28.092468598797716 86-87 27.62046344801548 21.211477653499458 22.73349379394969 28.43456510453537 88-89 26.70738916256158 20.835467980295565 22.32512315270936 30.1320197044335 90-91 23.405313961588217 22.390287590805055 24.450194049159123 29.75420439844761 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 2.0 10 4.5 11 7.5 12 8.5 13 16.0 14 27.5 15 46.0 16 75.5 17 116.5 18 172.5 19 236.0 20 343.5 21 495.5 22 666.0 23 930.0 24 1282.5 25 1632.5 26 2039.0 27 2588.0 28 3250.5 29 3959.5 30 4781.5 31 5740.0 32 6731.5 33 7783.0 34 9098.5 35 10514.0 36 12009.5 37 13601.0 38 15366.0 39 17509.0 40 19732.5 41 22145.0 42 24730.5 43 27309.5 44 30099.0 45 33015.5 46 35961.0 47 38746.5 48 41417.5 49 43886.5 50 46019.5 51 47892.5 52 49023.5 53 49801.0 54 50103.5 55 49663.0 56 48847.5 57 47646.5 58 46209.5 59 44433.5 60 42310.5 61 39923.5 62 37393.0 63 34701.0 64 31872.0 65 29096.5 66 25904.5 67 22896.0 68 20264.5 69 17541.0 70 14894.5 71 12410.0 72 10217.5 73 8165.5 74 6349.0 75 4978.5 76 3825.5 77 2791.5 78 2115.0 79 1569.0 80 1096.5 81 780.5 82 560.5 83 388.5 84 258.5 85 174.0 86 111.5 87 78.0 88 66.0 89 49.0 90 28.5 91 19.5 92 13.0 93 7.0 94 4.5 95 5.0 96 5.0 97 3.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0013584926165926287 2 0.00513208321823882 3 0.0015094362406584765 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 10768.0 32-33 23183.0 34-35 25782.0 36-37 27129.0 38-39 28784.0 40-41 29956.0 42-43 30448.0 44-45 30976.0 46-47 30945.0 48-49 30717.0 50-51 30538.0 52-53 29779.0 54-55 28365.0 56-57 27748.0 58-59 26826.0 60-61 24966.0 62-63 24251.0 64-65 22498.0 66-67 21273.0 68-69 20235.0 70-71 18883.0 72-73 17430.0 74-75 16152.0 76-77 14742.0 78-79 13434.0 80-81 12322.0 82-83 11191.0 84-85 9739.0 86-87 8734.0 88-89 7940.0 90-91 6765.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.83962447021112 #Duplication Level Percentage of deduplicated Percentage of total 1 99.84767743770696 99.68754619603435 2 0.14568135250984554 0.29089543053790856 3 0.005455310073674005 0.016339683273725175 4 9.441625785216555E-4 0.003770593491137132 5 0.0 0.0 6 2.4173713101522905E-4 0.0014480966628640014 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001660379864724324 0.0 0.0 0.0 0.0 2 0.001660379864724324 0.0 0.0 0.0 0.0 3 0.001660379864724324 0.0 0.0 0.0 1.5094362406584765E-4 4 0.001660379864724324 0.0 0.0 0.0 1.5094362406584765E-4 5 0.001660379864724324 0.0 0.0 0.0 1.5094362406584765E-4 6 0.002113210736921867 0.0 0.0 0.0 1.5094362406584765E-4 7 0.0028679288572511052 0.0 0.0 0.0 1.5094362406584765E-4 8 0.007547181203292382 0.0 0.0 0.0 1.5094362406584765E-4 9 0.00769812482735823 0.0 0.0 0.0 1.5094362406584765E-4 10-11 0.007849068451424078 0.0 0.0 0.0 1.5094362406584765E-4 12-13 0.007849068451424078 0.0 0.0 0.0 1.5094362406584765E-4 14-15 0.007849068451424078 0.0 0.0 0.0 1.5094362406584765E-4 16-17 0.007849068451424078 0.0 0.0 0.0 1.5094362406584765E-4 18-19 0.007924540263457002 0.0 0.0 0.0 3.018872481316953E-4 20-21 0.008150955699555772 0.0 0.0 0.0 3.018872481316953E-4 22-23 0.008452842947687469 1.5094362406584765E-4 0.0 0.0 3.018872481316953E-4 24-25 0.008603786571753315 1.5094362406584765E-4 0.0 0.0 3.018872481316953E-4 26-27 0.008905673819885012 1.5094362406584765E-4 0.0 0.0 3.018872481316953E-4 28-29 0.009207561068016706 1.5094362406584765E-4 0.0 0.0 3.018872481316953E-4 30-31 0.009584920128181325 1.5094362406584765E-4 0.0 0.0 3.018872481316953E-4 32-33 0.010566053684609335 1.5094362406584765E-4 0.0 0.0 3.773590601646191E-4 34-35 0.01124529999290565 1.5094362406584765E-4 0.0 0.0 4.5283087219754296E-4 36-37 0.011698130865103192 1.5094362406584765E-4 0.0 7.547181203292382E-5 4.5283087219754296E-4 38-39 0.011924546301201964 1.5094362406584765E-4 0.0 1.5094362406584765E-4 4.5283087219754296E-4 40-41 0.012150961737300736 1.5094362406584765E-4 0.0 1.5094362406584765E-4 4.5283087219754296E-4 42-43 0.012377377173399507 1.5094362406584765E-4 0.0 1.5094362406584765E-4 4.5283087219754296E-4 44-45 0.01245284898543243 1.5094362406584765E-4 7.547181203292382E-5 1.5094362406584765E-4 4.5283087219754296E-4 46-47 0.012528320797465355 1.5094362406584765E-4 1.5094362406584765E-4 1.5094362406584765E-4 4.5283087219754296E-4 48-49 0.012528320797465355 1.5094362406584765E-4 1.5094362406584765E-4 1.5094362406584765E-4 6.037744962633906E-4 50-51 0.012528320797465355 1.5094362406584765E-4 1.5094362406584765E-4 1.5094362406584765E-4 6.037744962633906E-4 52-53 0.012603792609498279 1.5094362406584765E-4 1.5094362406584765E-4 1.5094362406584765E-4 6.037744962633906E-4 54-55 0.012981151669662898 1.5094362406584765E-4 3.018872481316953E-4 1.5094362406584765E-4 6.037744962633906E-4 56-57 0.013132095293728746 1.5094362406584765E-4 3.018872481316953E-4 1.5094362406584765E-4 6.037744962633906E-4 58-59 0.013132095293728746 1.5094362406584765E-4 3.018872481316953E-4 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