##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-6C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 646483 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.23877348669648 40.0 40.0 40.0 40.0 40.0 2 39.1335858792884 40.0 40.0 40.0 40.0 40.0 3 39.12094826932804 40.0 40.0 40.0 40.0 40.0 4 39.07308312824931 40.0 40.0 40.0 40.0 40.0 5 39.183526867682524 40.0 40.0 40.0 40.0 40.0 6 39.235184838580444 40.0 40.0 40.0 40.0 40.0 7 39.27815580610782 40.0 40.0 40.0 40.0 40.0 8 39.29249183659895 40.0 40.0 40.0 40.0 40.0 9 39.31369579710526 40.0 40.0 40.0 40.0 40.0 10-11 39.301082936442256 40.0 40.0 40.0 40.0 40.0 12-13 39.29789646440819 40.0 40.0 40.0 40.0 40.0 14-15 39.297952150327234 40.0 40.0 40.0 40.0 40.0 16-17 39.438481754353944 40.0 40.0 40.0 40.0 40.0 18-19 39.45573201460827 40.0 40.0 40.0 40.0 40.0 20-21 39.45978471204966 40.0 40.0 40.0 40.0 40.0 22-23 39.46993501762614 40.0 40.0 40.0 40.0 40.0 24-25 39.46936578378704 40.0 40.0 40.0 40.0 40.0 26-27 39.45324780388657 40.0 40.0 40.0 40.0 40.0 28-29 39.40648710020217 40.0 40.0 40.0 40.0 40.0 30-31 39.25762194545308 40.0 40.0 40.0 40.0 40.0 32-33 39.21190925326698 40.0 40.0 40.0 40.0 40.0 34-35 39.18155648957755 40.0 40.0 40.0 40.0 40.0 36-37 39.16158319481101 40.0 40.0 40.0 40.0 40.0 38-39 39.11761130897437 40.0 40.0 40.0 40.0 40.0 40-41 39.07269100469012 40.0 40.0 40.0 40.0 40.0 42-43 39.040475408446426 40.0 40.0 40.0 40.0 40.0 44-45 39.02266860836197 40.0 40.0 40.0 40.0 40.0 46-47 38.95486875464859 40.0 40.0 40.0 40.0 40.0 48-49 38.91810124262045 40.0 40.0 40.0 40.0 40.0 50-51 38.87021078603607 40.0 40.0 40.0 40.0 40.0 52-53 38.813297635969604 40.0 40.0 40.0 40.0 40.0 54-55 38.76153867879435 40.0 40.0 40.0 40.0 40.0 56-57 38.73419720425445 40.0 40.0 40.0 40.0 40.0 58-59 38.687648489530524 40.0 40.0 40.0 40.0 40.0 60-61 38.62623267160885 40.0 40.0 40.0 40.0 40.0 62-63 39.342152107568594 40.0 40.0 40.0 40.0 40.0 64-65 39.34678601697989 40.0 40.0 40.0 40.0 40.0 66-67 39.334838250374986 40.0 40.0 40.0 40.0 40.0 68-69 39.34347726623205 40.0 40.0 40.0 40.0 40.0 70-71 39.337873310406216 40.0 40.0 40.0 40.0 40.0 72-73 39.34806244089768 40.0 40.0 40.0 40.0 40.0 74-75 39.33912686530007 40.0 40.0 40.0 40.0 40.0 76-77 39.33708822627012 40.0 40.0 40.0 40.0 40.0 78-79 39.327193602737026 40.0 40.0 40.0 40.0 40.0 80-81 39.299017724837995 40.0 40.0 40.0 40.0 40.0 82-83 39.30251857227682 40.0 40.0 40.0 40.0 40.0 84-85 39.2897732241113 40.0 40.0 40.0 40.0 40.0 86-87 39.33493481960551 40.0 40.0 40.0 40.0 40.0 88-89 39.320268300879164 40.0 40.0 40.0 40.0 40.0 90-91 39.45766854738572 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2336977952607171 1101 2 0.035241421267805606 1101 3 0.1500785525863364 1101 4 8.178873653719165E-4 1101 5 0.1771410290688138 1101 6 -0.08818602658456598 1101 7 0.05301613027139496 1101 8 0.007984696146934311 1101 9 0.15185725875179656 1101 10-11 -0.025534022227425623 1101 12-13 -0.013188782000177923 1101 14-15 -0.04617591366610441 1101 16-17 -0.03794255585238915 1101 18-19 -0.13249767111597777 1101 20-21 -0.06438109332740538 1101 22-23 -0.0358548122963569 1101 24-25 0.07390489283672252 1101 26-27 -0.004206281428423608 1101 28-29 0.023426369879857134 1101 30-31 -0.02661174002247435 1101 32-33 -0.042866023039238144 1101 34-35 -0.2247636315263577 1101 36-37 0.020844548723289336 1101 38-39 -0.10451328461969212 1101 40-41 0.11090490187582702 1101 42-43 0.03960254680274744 1101 44-45 0.03155246229117381 1101 46-47 -0.22759689659739024 1101 48-49 -0.11248369597350916 1101 50-51 -0.013292318819644322 1101 52-53 -0.04937837756635588 1101 54-55 0.08675518503579127 1101 56-57 0.03574889617173227 1101 58-59 0.2058427869863948 1101 60-61 -0.03372696450986723 1101 62-63 0.0174441899044524 1101 64-65 -0.19588535600072277 1101 66-67 -0.2535325297164164 1101 68-69 -0.17727505013946399 1101 70-71 -0.20718947995919024 1101 72-73 0.0010614674988786987 1101 74-75 -0.2804975881795073 1101 76-77 -0.2344547474729879 1101 78-79 -0.21008300156817938 1101 80-81 -0.24263507627667025 1101 82-83 -0.5006697363020223 1101 84-85 -0.47098384671031823 1101 86-87 -0.5395120686141084 1101 88-89 -0.7657674821324463 1101 90-91 -0.5795688588945183 1102 1 0.28946929656281384 1102 2 0.23801224430859236 1102 3 0.27793164621152044 1102 4 -0.0790662220607885 1102 5 0.12416630895627634 1102 6 -0.092180232055874 1102 7 0.1981958695176047 1102 8 0.05875213625478892 1102 9 0.06881127933409914 1102 10-11 0.02662427223846464 1102 12-13 -0.009961597956902324 1102 14-15 -0.07832298751284839 1102 16-17 0.07802756715574333 1102 18-19 0.0026278909616834767 1102 20-21 0.11844466050560953 1102 22-23 0.10036434146799422 1102 24-25 0.08011223983018567 1102 26-27 0.06346744976238483 1102 28-29 -0.06108247682471557 1102 30-31 2.968119315056583E-4 1102 32-33 0.06383750967854951 1102 34-35 0.09867619606649924 1102 36-37 -0.02115215181257213 1102 38-39 0.03375393903700541 1102 40-41 0.03984438691578873 1102 42-43 0.02032408925519036 1102 44-45 0.0751948643062974 1102 46-47 0.10546337099861347 1102 48-49 -0.009166750649171718 1102 50-51 -0.09991362381142466 1102 52-53 0.014081337550805983 1102 54-55 -0.15430321474270414 1102 56-57 0.09340392899304106 1102 58-59 -0.1294686196131707 1102 60-61 0.1362261289457507 1102 62-63 0.06687172446210354 1102 64-65 0.008979355842519965 1102 66-67 0.07708835163006 1102 68-69 -0.12201090026142225 1102 70-71 -0.0010496815106648683 1102 72-73 0.11435448976953211 1102 74-75 0.060265398456273545 1102 76-77 0.04735316397110978 1102 78-79 0.17274784339313953 1102 80-81 0.2722511392185396 1102 82-83 0.07301614250148702 1102 84-85 0.022535167823996005 1102 86-87 0.5557166157450197 1102 88-89 0.7048207531616697 1102 90-91 -0.5795688588945183 1103 1 0.30955284412065254 1103 2 0.03578430943284161 1103 3 0.09845837071880226 1103 4 -0.019404696782295616 1103 5 0.034332600730053286 1103 6 -0.03555132245861614 1103 7 -0.0871779522476075 1103 8 -0.028008789195084205 1103 9 0.1131289742783963 1103 10-11 -0.18522033465956156 1103 12-13 -0.05308766907943152 1103 14-15 -0.02614927821551305 1103 16-17 -0.0714107629642271 1103 18-19 -0.06564947621912154 1103 20-21 0.044078631156857284 1103 22-23 -0.0016299548044997891 1103 24-25 -0.1549623370764195 1103 26-27 5.030109057457821E-5 1103 28-29 -0.0282693073731366 1103 30-31 -7.808095624284306E-5 1103 32-33 0.08073805129102851 1103 34-35 -0.0991789308396207 1103 36-37 -0.1483222095160457 1103 38-39 -0.10024558048986165 1103 40-41 -0.1909060728799119 1103 42-43 0.06448921782979511 1103 44-45 -0.14570846442971686 1103 46-47 0.0674299650540604 1103 48-49 -0.014564596021315879 1103 50-51 0.04229026542124359 1103 52-53 -0.060454419733794396 1103 54-55 -0.17466871722877642 1103 56-57 -0.1346583132040564 1103 58-59 0.2168457206789114 1103 60-61 -0.015928474006784654 1103 62-63 -0.05833709446881841 1103 64-65 0.023864234363948356 1103 66-67 0.03964602919140958 1103 68-69 0.06147482924232861 1103 70-71 -0.1333188640169567 1103 72-73 -0.2893555437451525 1103 74-75 -0.17737365298800967 1103 76-77 0.042892067422641844 1103 78-79 -0.03470257690809575 1103 80-81 0.022026267959198265 1103 82-83 0.26957029845414127 1103 84-85 0.05751769404612617 1103 86-87 0.009040466307702388 1103 88-89 0.23423251786755372 1103 90-91 0.5632882839626205 1104 1 0.33481049558848497 1104 2 0.14332126550196733 1104 3 0.2518602159197201 1104 4 0.191895532241233 1104 5 0.08635575032405995 1104 6 0.0016926526611058534 1104 7 -0.012140215416458489 1104 8 -0.012549701125131207 1104 9 -0.034350465654974016 1104 10-11 0.05044373597337426 1104 12-13 0.015050083366951128 1104 14-15 -0.002315704147221709 1104 16-17 0.0014871979456216877 1104 18-19 -0.04330178987725475 1104 20-21 0.05255396558325032 1104 22-23 0.04758001162273473 1104 24-25 -0.002075306834896651 1104 26-27 -0.04198560094981474 1104 28-29 -0.009491167408526735 1104 30-31 -0.03668280716902217 1104 32-33 -0.038802010068799575 1104 34-35 -0.13125545924530257 1104 36-37 -0.19839786604296705 1104 38-39 -0.03290060249319282 1104 40-41 -0.10068150423765587 1104 42-43 -0.07726543281865617 1104 44-45 -0.03854629508735741 1104 46-47 -0.054408341332560894 1104 48-49 0.03340878755980725 1104 50-51 -0.14879087177865813 1104 52-53 -0.1664757151654186 1104 54-55 0.0320714250396108 1104 56-57 -0.03441999303099408 1104 58-59 0.10734032792141335 1104 60-61 -0.08902304581202714 1104 62-63 -0.22225420234675397 1104 64-65 -0.27002485086561734 1104 66-67 -0.1820156242687645 1104 68-69 0.07840903718516046 1104 70-71 -0.039571665349399154 1104 72-73 -0.2279767361000964 1104 74-75 -0.29979195011018334 1104 76-77 -0.36018117774383285 1104 78-79 -0.12121163439172733 1104 80-81 -0.5217826700353783 1104 82-83 -0.31430611271639464 1104 84-85 -0.4350503371863397 1104 86-87 -0.3346909077659532 1104 88-89 -1.1560815024774342 1104 90-91 -1.1256006049262695 1201 1 0.20644528057446365 1201 2 0.12268242887900982 1201 3 0.10264473412033226 1201 4 0.05114190375500982 1201 5 0.13647631585714493 1201 6 0.17595447677676646 1201 7 0.010627637750324936 1201 8 -0.004605193410831987 1201 9 0.13700400011018132 1201 10-11 -0.06502423913362776 1201 12-13 -0.05919365688683342 1201 14-15 -0.08997557884580942 1201 16-17 -0.020434160102063004 1201 18-19 -0.06991333497092711 1201 20-21 -0.1038462693907789 1201 22-23 -0.11867457275242543 1201 24-25 -0.0712439487213885 1201 26-27 -0.05010049194518018 1201 28-29 0.023701819150943493 1201 30-31 -0.017346628176738932 1201 32-33 -0.10335824417229844 1201 34-35 -0.23226556808883458 1201 36-37 -0.16684171865548336 1201 38-39 -0.21005852728844587 1201 40-41 -0.06943501195536328 1201 42-43 -0.12537332564391335 1201 44-45 -0.008521073891067488 1201 46-47 -0.13980137143639126 1201 48-49 0.09132116470308205 1201 50-51 -0.08851836602777752 1201 52-53 -0.08958857679166954 1201 54-55 -0.16917745524700223 1201 56-57 -0.11674020366918114 1201 58-59 -0.39910601545846447 1201 60-61 -0.0932935716015777 1201 62-63 -0.04254502529779103 1201 64-65 -4.4200952284967343E-4 1201 66-67 -0.001557325370789897 1201 68-69 -0.2180085611525513 1201 70-71 -0.015471000916761568 1201 72-73 -0.12683091891606324 1201 74-75 0.2532048147868906 1201 76-77 -0.10594942697291998 1201 78-79 -0.09481961715385978 1201 80-81 0.09058737172506426 1201 82-83 0.08234318864638368 1201 84-85 -0.07018500865657273 1201 86-87 -0.34730489105007933 1201 88-89 -0.5611890990550847 1201 90-91 0.06328828396262054 1202 1 0.12121900543053954 1202 2 0.3160551806679379 1202 3 0.2283682277028376 1202 4 0.13601778148736798 1202 5 0.008355682901068917 1202 6 0.07224285835804523 1202 7 0.22248573281405015 1202 8 0.025811708075991646 1202 9 0.053932744239993724 1202 10-11 0.05226339916902134 1202 12-13 -0.07168164017302558 1202 14-15 0.04766518635898365 1202 16-17 0.06611024092702422 1202 18-19 0.00297007856155318 1202 20-21 0.06280604802566359 1202 22-23 -0.019536166902739183 1202 24-25 -0.05318269158419753 1202 26-27 0.03784625065596714 1202 28-29 -0.07124911205273321 1202 30-31 0.01835480766312969 1202 32-33 -0.15350283294332456 1202 34-35 0.03105946402555304 1202 36-37 0.01050964002680388 1202 38-39 0.017849603075795528 1202 40-41 0.08474152913188959 1202 42-43 -0.010539943640445415 1202 44-45 0.14150093069230252 1202 46-47 -0.07238531467761788 1202 48-49 -0.05903916976644297 1202 50-51 -0.04233345264674426 1202 52-53 0.002537922243313062 1202 54-55 -0.22391695747275975 1202 56-57 -0.09857656205599596 1202 58-59 -0.38904752560588207 1202 60-61 -0.11294194988306572 1202 62-63 -0.14631644818146583 1202 64-65 -0.1290354834921601 1202 66-67 -0.10783311458060041 1202 68-69 -0.2653481812238496 1202 70-71 -0.1638564517349934 1202 72-73 -0.08457836897266446 1202 74-75 -0.48362386243361044 1202 76-77 0.11839286641942692 1202 78-79 -0.43553009833107126 1202 80-81 -0.41009381731181094 1202 82-83 -0.693133664249828 1202 84-85 -0.3774499659215351 1202 86-87 -0.982404722376323 1202 88-89 -0.3254231492773556 1202 90-91 -0.8336347929604528 1203 1 0.1439904177366742 1203 2 0.06346393251882887 1203 3 0.08880547024766372 1203 4 0.15918776493737852 1203 5 -0.05528570021223089 1203 6 0.13916709626930412 1203 7 -0.11194974258918222 1203 8 0.11657307181787502 1203 9 -0.0344343354978065 1203 10-11 0.09911693713078051 1203 12-13 -0.025794530092390744 1203 14-15 -0.029669044116566567 1203 16-17 -0.04228182450721363 1203 18-19 0.0893214454534288 1203 20-21 -0.07801813709991734 1203 22-23 -4.260939813605091E-5 1203 24-25 -0.022108428065813257 1203 26-27 -0.07386938383287145 1203 28-29 -0.04662270107159827 1203 30-31 0.027086229997827616 1203 32-33 0.09426058972957918 1203 34-35 -0.07079509848289689 1203 36-37 -0.10571584526935851 1203 38-39 -0.02979468296661736 1203 40-41 -0.11003152395515059 1203 42-43 -0.15861243879466969 1203 44-45 -0.2497244709941029 1203 46-47 -0.046317162504891485 1203 48-49 -0.19804106686895295 1203 50-51 -0.09018505071738048 1203 52-53 -0.0869109052783017 1203 54-55 0.013889067037531788 1203 56-57 -0.16122916359778117 1203 58-59 -0.1037489644350984 1203 60-61 -0.26009484351062895 1203 62-63 -0.24602536765865324 1203 64-65 -0.11969480522174791 1203 66-67 -0.005506505355185709 1203 68-69 0.1320219456003855 1203 70-71 -0.1803918466016512 1203 72-73 -0.039026955187416945 1203 74-75 -0.21232511974071855 1203 76-77 -0.003346152620466114 1203 78-79 -0.2650378641498179 1203 80-81 -0.3512479429109234 1203 82-83 -0.07296060059849907 1203 84-85 -0.277039353821813 1203 86-87 -0.8322736548539496 1203 88-89 -0.08002773168681188 1203 90-91 0.5632882839626205 1204 1 0.2515411744617637 1204 2 0.16055989291578499 1204 3 -0.14390679235719972 1204 4 0.06041277778383147 1204 5 -0.12454026731303713 1204 6 0.01904267065928167 1204 7 -0.015196354402000622 1204 8 -0.03204828650208924 1204 9 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0.1844020650475784 2401 50-51 0.08548384009387888 2401 52-53 0.08020534101395072 2401 54-55 0.08747188366252345 2401 56-57 0.2429670309381322 2401 58-59 0.21654982026449687 2401 60-61 -0.0737334389895139 2401 62-63 0.11152424006360206 2401 64-65 0.19176983136659942 2401 66-67 0.11590587233796157 2401 68-69 0.06216512071650726 2401 70-71 -0.016139409631861668 2401 72-73 0.007569348651493613 2401 74-75 -0.01963975075283031 2401 76-77 -0.06865790467921329 2401 78-79 -0.3399294671593296 2401 80-81 -0.0923589240402336 2401 82-83 -0.06483066154387984 2401 84-85 -0.12597386838243807 2401 86-87 0.21570107572947705 2401 88-89 0.4259745993155164 2401 90-91 -0.13115616048182233 2402 1 0.2964140520184486 2402 2 0.39215287311848357 2402 3 0.2977935671957681 2402 4 0.30091082780152334 2402 5 0.2070575087827038 2402 6 0.15941637498621475 2402 7 0.0861790938228566 2402 8 0.006544056333368076 2402 9 0.15727579967522587 2402 10-11 0.14963890120957757 2402 12-13 0.12991779936810133 2402 14-15 0.10304430724713143 2402 16-17 0.005917259199925695 2402 18-19 0.062396279684868716 2402 20-21 0.03343360841198262 2402 22-23 0.07127310123498631 2402 24-25 0.11649557281518241 2402 26-27 0.10572018591442145 2402 28-29 0.053631885522726463 2402 30-31 0.08797360313202773 2402 32-33 0.12675692474272893 2402 34-35 0.1891364387309764 2402 36-37 0.05619754830182444 2402 38-39 0.05209145223772538 2402 40-41 0.10442247745693578 2402 42-43 0.15309905226805398 2402 44-45 0.14058826447033823 2402 46-47 0.20658869854224804 2402 48-49 0.17494462912979714 2402 50-51 0.2577922131659207 2402 52-53 0.1675122005077796 2402 54-55 0.18983547224159025 2402 56-57 0.16694833864738712 2402 58-59 0.2370708218996711 2402 60-61 0.06749748528598332 2402 62-63 0.08503455160201412 2402 64-65 0.23189432337844806 2402 66-67 0.009956470727139788 2402 68-69 0.2449802162394903 2402 70-71 0.14896580355092937 2402 72-73 0.21615328877926743 2402 74-75 0.22607383397380687 2402 76-77 0.12983545310891742 2402 78-79 0.2957402297160172 2402 80-81 0.10072897249905566 2402 82-83 0.27003858716430784 2402 84-85 -0.11824603853578708 2402 86-87 -0.1940942620429098 2402 88-89 0.1022566505975675 2402 90-91 0.5632882839626205 2403 1 -0.24761585351834015 2403 2 -0.10270261618279619 2403 3 -0.08749261620680926 2403 4 0.10115255125020184 2403 5 0.09224506264931875 2403 6 0.11743999830807894 2403 7 0.1787286864931943 2403 8 0.1325014926023229 2403 9 0.03459539976709891 2403 10-11 0.15325885580755738 2403 12-13 0.1370026139873346 2403 14-15 0.14200194195458948 2403 16-17 0.06441150315662014 2403 18-19 0.08936091664708812 2403 20-21 0.02861329255568279 2403 22-23 0.14281799359611114 2403 24-25 0.09362649416247848 2403 26-27 0.09216798641679702 2403 28-29 0.0712651953559913 2403 30-31 0.007839537728706603 2403 32-33 0.14607035249065348 2403 34-35 0.1226119129745058 2403 36-37 0.09876504731001035 2403 38-39 0.143700041113199 2403 40-41 0.13640922654658993 2403 42-43 0.1495939529193393 2403 44-45 0.026381757046664234 2403 46-47 0.12176267346963954 2403 48-49 0.09972468665102951 2403 50-51 0.061356320006602516 2403 52-53 -0.004928430245129789 2403 54-55 -0.06668774129862953 2403 56-57 -0.01945497024119902 2403 58-59 0.005737753169022142 2403 60-61 0.13750285642980487 2403 62-63 0.0673408133660871 2403 64-65 0.15459766915532924 2403 66-67 0.12297189178293166 2403 68-69 0.19236099640108506 2403 70-71 0.38811014254737586 2403 72-73 0.27655662086957733 2403 74-75 0.15208263162821112 2403 76-77 0.1922918704375718 2403 78-79 0.28124769098650404 2403 80-81 0.35070345965967675 2403 82-83 0.36166774349275954 2403 84-85 -0.05274911122067749 2403 86-87 0.22249958916018642 2403 88-89 0.39375677295664957 2403 90-91 0.19965192032626078 2404 1 -0.3523000298801122 2404 2 -0.2713467653038677 2404 3 -0.31552878985726096 2404 4 -0.2685323455578299 2404 5 -0.15719111929278995 2404 6 -0.04980732582112779 2404 7 -0.1225517943996195 2404 8 0.02004983280720296 2404 9 -0.1250427361644384 2404 10-11 0.021756393388905337 2404 12-13 0.18441986486296003 2404 14-15 0.14450393441613585 2404 16-17 0.007498857483689392 2404 18-19 0.048423333386253375 2404 20-21 -0.013830124870302996 2404 22-23 -0.029092661789647423 2404 24-25 0.04735325977043203 2404 26-27 0.007829096611445152 2404 28-29 0.01894224176403725 2404 30-31 -0.010157702831634197 2404 32-33 0.028763603806844174 2404 34-35 0.08099434784519843 2404 36-37 -0.004633178867798904 2404 38-39 0.06941074028572558 2404 40-41 0.06449561108544799 2404 42-43 0.20075825344962794 2404 44-45 -0.026526328110790587 2404 46-47 0.0954734688226111 2404 48-49 0.08937959175753463 2404 50-51 0.13332608536947532 2404 52-53 -0.10129109716953622 2404 54-55 0.16362095235114538 2404 56-57 0.09631559240676069 2404 58-59 0.015805540892941394 2404 60-61 0.2717365181621787 2404 62-63 0.12196797571120044 2404 64-65 0.1086561024053907 2404 66-67 -0.012043567542036726 2404 68-69 0.04823242420393825 2404 70-71 0.07135627428878877 2404 72-73 0.2605200759906765 2404 74-75 0.19045358740193308 2404 76-77 0.10231050872405945 2404 78-79 0.08134703408570232 2404 80-81 0.01871126042485116 2404 82-83 -0.1091597942347633 2404 84-85 0.3420212503923139 2404 86-87 0.26410022298446023 2404 88-89 0.2491245506300288 2404 90-91 0.5632882839626205 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 20.0 31 479.0 32 3099.0 33 5374.0 34 6952.0 35 8922.0 36 13277.0 37 22869.0 38 83010.0 39 351179.0 40 151302.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 29.589514895302088 15.539561223179716 25.288060401033906 29.582863480484285 2 31.613452137862758 16.03907555225543 24.907029267990843 27.440443041890973 3 28.316774404118313 19.44038398772427 19.684630127196705 32.558211480960715 4 27.066295633450533 20.93496658071442 19.93973546094793 32.05900232488712 5 27.000555312359335 21.125226804107765 20.67478959230173 31.19942829123117 6 27.32492579077872 23.114760944990046 23.691574256399626 25.868739007831604 7 26.347483228483963 23.041595834693258 23.29589486498485 27.315026071837927 8 25.446763487980352 23.69946309493057 23.827695391835515 27.026078025253565 9 25.292544428855823 23.655378409022358 24.225540346768593 26.826536815353226 10-11 25.54452321252067 23.162867391717956 23.7857762694456 27.506833126315772 12-13 25.42417975414667 22.865566457277296 23.9411554518835 27.769098336692533 14-15 25.190221552616233 21.998258268198853 24.10218056777982 28.709339611405092 16-17 25.94383765698402 22.263462457636162 23.555917170289085 28.23678271509073 18-19 25.839967949659933 22.27483166610723 23.791190178241344 28.094010205991495 20-21 25.97268605670992 22.376922517684147 23.70224739088267 27.948144034723267 22-23 25.825118371248738 22.049303693987312 23.833496008402385 28.29208192636156 24-25 25.54924104732839 22.19602062235202 24.10751713502134 28.14722119529825 26-27 25.89820613999131 22.388291726155213 23.90202062544568 27.811481508407798 28-29 25.98946917397673 21.991993602306632 23.9070478264703 28.11148939724633 30-31 26.038776021606168 22.157229435622337 23.973443962863623 27.83055057990787 32-33 26.082736867645274 22.158868804507527 23.933795282033714 27.824599045813482 34-35 26.06030452427847 21.93989284495413 23.996398011504983 28.00340461926241 36-37 26.02242035381185 22.18716158663168 24.035406866500875 27.755011193055594 38-39 26.176783107701993 22.208885486132974 23.902372706717422 27.71195869944761 40-41 26.23537564102444 21.898866996043907 23.88807049885743 27.97768686407422 42-43 26.216727447918004 22.159949137248475 24.022481494132816 27.600841920700702 44-45 26.332713676998914 22.109407636394018 23.74248057481828 27.815398111788785 46-47 26.394172085487916 21.929581215739365 23.778379458043897 27.897867240728825 48-49 26.460656705658614 22.138502434131432 23.880453675621457 27.520387184588497 50-51 26.41918616941348 22.217977419346624 23.666898432903675 27.69593797833622 52-53 26.608581481776504 21.95361791777797 23.66980440198537 27.767996198460153 54-55 26.50462014385702 22.268780463817443 23.589481479936165 27.637117912389375 56-57 26.48276175748203 22.250360619095268 23.685039830541513 27.581837792881185 58-59 26.572757427811723 22.061201077821945 23.650721660486088 27.715319833880248 60-61 26.579107286377983 22.204732127104858 23.609080038421578 27.607080548095585 62-63 26.722288283157212 22.07024584087841 23.60194168425675 27.605524191707627 64-65 26.72102131048431 22.013387946962943 23.453429902661806 27.812160839890936 66-67 26.77117169294318 22.248479003832884 23.374576210991606 27.605773092232333 68-69 26.84039996899465 22.14557011084412 23.33090458104023 27.683125339121002 70-71 26.894446184064446 21.92953115659427 23.30731019955023 27.868712459791055 72-73 26.837693003730955 22.09817831747435 23.347202194778312 27.71692648401638 74-75 27.025004252423884 21.987218428790126 23.060773212159503 27.92700410662649 76-77 27.063004771595384 21.958035470984875 23.05150192197311 27.927457835446635 78-79 27.03779864747408 21.792344166260342 23.153349868916635 28.01650731734894 80-81 27.53584845759035 21.759528722860118 22.651110288022295 28.053512531527243 82-83 28.06145606390704 21.3496278140886 22.258533042846768 28.33038307915759 84-85 28.352777480466663 20.852892157612267 22.17397287503249 28.62035748688858 86-87 28.241819190238495 20.716583471991125 21.368829728230725 29.672767609539658 88-89 28.1177872086963 19.421487603305785 22.261931352840445 30.198793835157474 90-91 25.213120830244623 20.237212750185325 24.91660489251297 29.63306152705708 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 2.0 10 3.5 11 4.5 12 4.5 13 4.5 14 8.5 15 14.0 16 28.0 17 52.0 18 75.0 19 99.0 20 130.5 21 183.5 22 255.0 23 353.0 24 472.5 25 622.0 26 835.5 27 1098.0 28 1420.0 29 1788.5 30 2272.5 31 2885.0 32 3569.5 33 4334.5 34 5324.0 35 6424.0 36 7739.0 37 9305.0 38 11095.5 39 13184.5 40 15425.0 41 18168.0 42 21222.5 43 24353.5 44 27709.5 45 31064.0 46 34421.0 47 37767.0 48 40774.0 49 43753.0 50 46481.0 51 48348.5 52 49732.0 53 50730.5 54 50925.0 55 50305.0 56 49569.0 57 48753.0 58 47364.5 59 45283.0 60 42951.5 61 40685.0 62 38378.0 63 35886.5 64 33183.0 65 30269.5 66 26937.0 67 23940.5 68 21102.0 69 18241.5 70 15701.0 71 13093.0 72 10658.5 73 8499.5 74 6576.5 75 5139.0 76 3929.0 77 2825.0 78 2103.0 79 1552.5 80 1072.5 81 744.5 82 516.0 83 353.5 84 241.0 85 162.5 86 104.5 87 69.5 88 53.0 89 39.5 90 30.5 91 22.0 92 12.5 93 6.0 94 3.5 95 3.0 96 2.0 97 1.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.187324337994967E-4 2 0.00665137366334459 3 6.187324337994967E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 10378.0 32-33 22369.0 34-35 23440.0 36-37 24922.0 38-39 25576.0 40-41 26583.0 42-43 27505.0 44-45 27946.0 46-47 28838.0 48-49 29072.0 50-51 28638.0 52-53 28650.0 54-55 27973.0 56-57 26927.0 58-59 26225.0 60-61 25207.0 62-63 24429.0 64-65 23201.0 66-67 22016.0 68-69 21074.0 70-71 19728.0 72-73 18539.0 74-75 17008.0 76-77 15792.0 78-79 14447.0 80-81 12995.0 82-83 11643.0 84-85 10420.0 86-87 9404.0 88-89 8284.0 90-91 7254.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.77232860636862 #Duplication Level Percentage of deduplicated Percentage of total 1 99.78312104988373 99.5559434275806 2 0.2085047230344578 0.416060034851476 3 0.006654433701185669 0.01991785037771971 4 9.501735930968132E-4 0.0037920412785419693 5 5.454554320704334E-4 0.0027210679304330025 6 0.0 0.0 7 2.2416435543205098E-4 0.0015655779812400981 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0013921479760488674 0.0 0.0 0.0 0.0 2 0.0013921479760488674 0.0 0.0 0.0 0.0 3 0.0013921479760488674 0.0 0.0 0.0 0.0 4 0.0013921479760488674 0.0 0.0 0.0 0.0 5 0.0013921479760488674 0.0 0.0 0.0 0.0 6 0.0015468310844987416 0.0 0.0 0.0 0.0 7 0.00185619730139849 0.0 0.0 0.0 0.0 8 0.005259225687295721 0.0 0.0 0.0 0.0 9 0.0054139087957455955 0.0 1.5468310844987416E-4 0.0 1.5468310844987416E-4 10-11 0.0054139087957455955 0.0 1.5468310844987416E-4 0.0 1.5468310844987416E-4 12-13 0.0054139087957455955 0.0 1.5468310844987416E-4 0.0 1.5468310844987416E-4 14-15 0.0054139087957455955 0.0 1.5468310844987416E-4 0.0 1.5468310844987416E-4 16-17 0.0054139087957455955 0.0 1.5468310844987416E-4 0.0 1.5468310844987416E-4 18-19 0.0054139087957455955 0.0 1.5468310844987416E-4 0.0 1.5468310844987416E-4 20-21 0.006032641229545092 0.0 1.5468310844987416E-4 0.0 1.5468310844987416E-4 22-23 0.006651373663344589 0.0 1.5468310844987416E-4 0.0 1.5468310844987416E-4 24-25 0.0068833983260194 1.5468310844987416E-4 1.5468310844987416E-4 0.0 1.5468310844987416E-4 26-27 0.00742478920559396 1.5468310844987416E-4 1.5468310844987416E-4 0.0 1.5468310844987416E-4 28-29 0.007734155422493708 1.5468310844987416E-4 1.5468310844987416E-4 0.0 1.5468310844987416E-4 30-31 0.008198204747843331 1.5468310844987416E-4 1.5468310844987416E-4 0.0 1.5468310844987416E-4 32-33 0.008971620290092703 1.5468310844987416E-4 1.5468310844987416E-4 0.0 1.5468310844987416E-4 34-35 0.00982237738656701 1.5468310844987416E-4 1.5468310844987416E-4 0.0 1.5468310844987416E-4 36-37 0.010518451374591443 1.5468310844987416E-4 1.5468310844987416E-4 0.0 1.5468310844987416E-4 38-39 0.011291866916840813 1.5468310844987416E-4 1.5468310844987416E-4 0.0 1.5468310844987416E-4 40-41 0.011755916242190436 1.5468310844987416E-4 3.093662168997483E-4 0.0 1.5468310844987416E-4 42-43 0.011755916242190436 1.5468310844987416E-4 3.093662168997483E-4 0.0 1.5468310844987416E-4 44-45 0.011755916242190436 1.5468310844987416E-4 3.093662168997483E-4 0.0 1.5468310844987416E-4 46-47 0.011910599350640311 1.5468310844987416E-4 3.093662168997483E-4 0.0 1.5468310844987416E-4 48-49 0.012142624013315121 1.5468310844987416E-4 3.093662168997483E-4 0.0 1.5468310844987416E-4 50-51 0.012297307121764996 1.5468310844987416E-4 3.093662168997483E-4 0.0 1.5468310844987416E-4 52-53 0.012529331784439808 1.5468310844987416E-4 3.093662168997483E-4 0.0 1.5468310844987416E-4 54-55 0.012838698001339556 1.5468310844987416E-4 3.093662168997483E-4 0.0 1.5468310844987416E-4 56-57 0.012838698001339556 1.5468310844987416E-4 3.093662168997483E-4 0.0 1.5468310844987416E-4 58-59 0.012838698001339556 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1.5468310844987416E-4 80 0.01407616286893855 3.093662168997483E-4 3.093662168997483E-4 0.0 1.5468310844987416E-4 >>END_MODULE