##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-6B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 563128 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.214317171229276 40.0 40.0 40.0 40.0 40.0 2 39.13120640422781 40.0 40.0 40.0 40.0 40.0 3 39.125999772698215 40.0 40.0 40.0 40.0 40.0 4 39.03847082723644 40.0 40.0 40.0 40.0 40.0 5 39.20097739767868 40.0 40.0 40.0 40.0 40.0 6 39.25953602022986 40.0 40.0 40.0 40.0 40.0 7 39.29277890639428 40.0 40.0 40.0 40.0 40.0 8 39.321553891832764 40.0 40.0 40.0 40.0 40.0 9 39.331619099032544 40.0 40.0 40.0 40.0 40.0 10-11 39.33168657925019 40.0 40.0 40.0 40.0 40.0 12-13 39.33716313165036 40.0 40.0 40.0 40.0 40.0 14-15 39.34019618985381 40.0 40.0 40.0 40.0 40.0 16-17 39.47426517594579 40.0 40.0 40.0 40.0 40.0 18-19 39.48425935133753 40.0 40.0 40.0 40.0 40.0 20-21 39.49688170362688 40.0 40.0 40.0 40.0 40.0 22-23 39.49491057095368 40.0 40.0 40.0 40.0 40.0 24-25 39.48727109999858 40.0 40.0 40.0 40.0 40.0 26-27 39.46949894162606 40.0 40.0 40.0 40.0 40.0 28-29 39.4710225739086 40.0 40.0 40.0 40.0 40.0 30-31 39.465749122942015 40.0 40.0 40.0 40.0 40.0 32-33 39.46326516743972 40.0 40.0 40.0 40.0 40.0 34-35 39.45657432893697 40.0 40.0 40.0 40.0 40.0 36-37 39.45117516784353 40.0 40.0 40.0 40.0 40.0 38-39 39.44262156439717 40.0 40.0 40.0 40.0 40.0 40-41 39.423287914838866 40.0 40.0 40.0 40.0 40.0 42-43 39.419877139717926 40.0 40.0 40.0 40.0 40.0 44-45 39.40272035216624 40.0 40.0 40.0 40.0 40.0 46-47 39.39646962277758 40.0 40.0 40.0 40.0 40.0 48-49 39.391528496550386 40.0 40.0 40.0 40.0 40.0 50-51 39.388323365889946 40.0 40.0 40.0 40.0 40.0 52-53 39.37181170765483 40.0 40.0 40.0 40.0 40.0 54-55 39.35144086499226 40.0 40.0 40.0 40.0 40.0 56-57 39.341356037629566 40.0 40.0 40.0 40.0 40.0 58-59 39.33132780920771 40.0 40.0 40.0 40.0 40.0 60-61 39.29180235137726 40.0 40.0 40.0 40.0 40.0 62-63 39.32053554417203 40.0 40.0 40.0 40.0 40.0 64-65 39.32672531243621 40.0 40.0 40.0 40.0 40.0 66-67 39.329553608790704 40.0 40.0 40.0 40.0 40.0 68-69 39.31798729476692 40.0 40.0 40.0 40.0 40.0 70-71 39.31888792589237 40.0 40.0 40.0 40.0 40.0 72-73 39.31757787351235 40.0 40.0 40.0 40.0 40.0 74-75 39.33770508176461 40.0 40.0 40.0 40.0 40.0 76-77 39.31314522956671 40.0 40.0 40.0 40.0 40.0 78-79 39.31013247218709 40.0 40.0 40.0 40.0 40.0 80-81 39.2846128032312 40.0 40.0 40.0 40.0 40.0 82-83 39.29445528342973 40.0 40.0 40.0 40.0 40.0 84-85 39.282420887219146 40.0 40.0 40.0 40.0 40.0 86-87 39.29769539946788 40.0 40.0 40.0 40.0 40.0 88-89 39.362786839400094 40.0 40.0 40.0 40.0 40.0 90-91 39.40426219387666 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18060942115285172 1101 2 0.24470675995152646 1101 3 0.2741691300171709 1101 4 0.27735272400538236 1101 5 0.06828351438442581 1101 6 0.06162819801237873 1101 7 0.09375321150451299 1101 8 -0.12104045645045858 1101 9 0.18404548477449367 1101 10-11 0.0258610926277143 1101 12-13 -0.13125260720283194 1101 14-15 -0.047092235727042464 1101 16-17 -0.03547154005835296 1101 18-19 0.05609966441897285 1101 20-21 -0.08881025772955553 1101 22-23 0.0628609078277762 1101 24-25 -0.08801976351635687 1101 26-27 0.10107616784719653 1101 28-29 0.12394421899800534 1101 30-31 -0.04520535430830108 1101 32-33 -0.15146266669074038 1101 34-35 -0.14145037889849732 1101 36-37 0.0017851333818370563 1101 38-39 -0.0185938462990336 1101 40-41 -0.005686281931296833 1101 42-43 0.02517765467929678 1101 44-45 0.11448541186754113 1101 46-47 -0.01710830289133014 1101 48-49 -0.024992485414337295 1101 50-51 0.09189479799544387 1101 52-53 0.022047716247733717 1101 54-55 -0.055389331010609055 1101 56-57 -0.05716234044150781 1101 58-59 0.022525464780265736 1101 60-61 -0.008370062560821623 1101 62-63 -0.00886823341568288 1101 64-65 0.029714693859411057 1101 66-67 0.07508242901620577 1101 68-69 0.19651356509375262 1101 70-71 0.15239115877203346 1101 72-73 0.14416200844048888 1101 74-75 0.031246707133902873 1101 76-77 -0.41786909926086224 1101 78-79 -0.38934855915900357 1101 80-81 -0.1813656925013447 1101 82-83 -0.24552854442747218 1101 84-85 0.2433023420831688 1101 86-87 0.2882881219603277 1101 88-89 -1.8591508197827338 1101 90-91 -1.8061555017050352 1102 1 0.33384135268800463 1102 2 0.09181875763573544 1102 3 0.08731811728892325 1102 4 0.1265712881582175 1102 5 0.18225879160570457 1102 6 0.005060439657050608 1102 7 0.11150855745431443 1102 8 -0.1313296722631776 1102 9 -0.035137740651045135 1102 10-11 -0.14583983798743105 1102 12-13 -0.0704301577962454 1102 14-15 -0.09431658362290563 1102 16-17 0.15465826456119203 1102 18-19 -0.09898231970869631 1102 20-21 0.022456576889219093 1102 22-23 0.021056311213762058 1102 24-25 0.08162905608990911 1102 26-27 -0.1296702445411384 1102 28-29 -0.015961888325108475 1102 30-31 -0.056258379595696795 1102 32-33 -0.01199639446652867 1102 34-35 -0.10939731797681418 1102 36-37 0.050086446297072484 1102 38-39 0.04174345410608282 1102 40-41 -0.02436890089267507 1102 42-43 0.03431472066844066 1102 44-45 -0.103399061699875 1102 46-47 -0.11331715351404625 1102 48-49 0.06052483266203268 1102 50-51 0.009930427219885019 1102 52-53 -0.1741022654045068 1102 54-55 -0.06383134712152128 1102 56-57 -0.18136328717919525 1102 58-59 -0.285547908097989 1102 60-61 -0.1790881316480153 1102 62-63 0.016351751271287185 1102 64-65 -0.027687385298484912 1102 66-67 -0.3969214129467744 1102 68-69 -0.2328783743054217 1102 70-71 -0.190040245893087 1102 72-73 -0.34250306842254474 1102 74-75 -0.25507327473469843 1102 76-77 -0.17861995241538153 1102 78-79 -0.5251064709169171 1102 80-81 -0.28769016588143614 1102 82-83 0.016597358565306308 1102 84-85 0.2710311540964341 1102 86-87 -0.556819245049418 1102 88-89 -0.7081458409887063 1102 90-91 0.06051116496163189 1103 1 0.21613049903383086 1103 2 0.16910538538108 1103 3 0.309962400065821 1103 4 0.04619352561131507 1103 5 0.04997859110859082 1103 6 -0.0529306805558889 1103 7 -0.09007857373586603 1103 8 -0.07653703703635273 1103 9 -0.20903402936248483 1103 10-11 0.0525529370698834 1103 12-13 0.0037887319826310772 1103 14-15 0.059181793737757005 1103 16-17 -0.004628765059194961 1103 18-19 -0.08909784502269247 1103 20-21 -0.018380534344835553 1103 22-23 0.04782229056398535 1103 24-25 0.015413260198393175 1103 26-27 0.02106868256258565 1103 28-29 -0.07940034226224668 1103 30-31 -0.07123373034178115 1103 32-33 0.0235553708628089 1103 34-35 -0.10962722076355647 1103 36-37 -0.01195735248033003 1103 38-39 -0.046529173850728966 1103 40-41 0.003645844891394745 1103 42-43 -0.07350860426970485 1103 44-45 -0.1087704344738043 1103 46-47 0.11013404793205694 1103 48-49 -0.06537247536981283 1103 50-51 -0.05236757705986861 1103 52-53 0.02941031958096829 1103 54-55 0.03641989556311387 1103 56-57 0.10439251456660514 1103 58-59 0.02532838726489217 1103 60-61 0.04081463707338884 1103 62-63 0.048135468519284075 1103 64-65 -0.17713684887838355 1103 66-67 -0.2124570431958901 1103 68-69 -0.13677465611366557 1103 70-71 -0.08133698776566689 1103 72-73 -0.09152414748800197 1103 74-75 0.11895555204699804 1103 76-77 0.05259040496333256 1103 78-79 -0.2059146727839476 1103 80-81 -0.4086891943435944 1103 82-83 0.07620970925270854 1103 84-85 0.07220112902015074 1103 86-87 0.30490611667594436 1103 88-89 0.45417855237016624 1103 90-91 0.5938444982949633 1104 1 0.4655604059957099 1104 2 0.24970319696367937 1104 3 0.1784829590872974 1104 4 0.1655629485424015 1104 5 0.3916459844291893 1104 6 0.21111686110780425 1104 7 -0.040993761331485246 1104 8 0.1633327465793144 1104 9 0.012524075206975738 1104 10-11 0.01604980717323201 1104 12-13 0.05309418294406498 1104 14-15 0.016664611675274443 1104 16-17 0.028970391493771785 1104 18-19 0.058497458943250535 1104 20-21 -0.005549147122856368 1104 22-23 0.08412327854909307 1104 24-25 0.13295151522486037 1104 26-27 0.11167846411720461 1104 28-29 0.10842252329092616 1104 30-31 0.17928351513777585 1104 32-33 0.21276037363128353 1104 34-35 0.07028887344055335 1104 36-37 -0.015291424645369034 1104 38-39 0.11167897537262661 1104 40-41 0.16147226222266653 1104 42-43 0.1508599349334716 1104 44-45 0.14225536064729738 1104 46-47 0.05874158841220378 1104 48-49 0.08065131701429351 1104 50-51 -0.035223251821399515 1104 52-53 -0.025475460569587938 1104 54-55 0.15083844752625453 1104 56-57 0.11313506498579784 1104 58-59 0.05445411179960047 1104 60-61 0.09934881180721788 1104 62-63 0.27803662513790073 1104 64-65 0.19532293771184328 1104 66-67 0.25665664291648227 1104 68-69 -0.05543049374939102 1104 70-71 -0.14547005719870754 1104 72-73 0.06423919430018543 1104 74-75 -0.3828315300990397 1104 76-77 0.18135047326966713 1104 78-79 0.2622121750718165 1104 80-81 0.14137395778547557 1104 82-83 0.10351949423844076 1104 84-85 -0.062109374842407306 1104 86-87 0.3756131873830171 1104 88-89 -0.0989875169249288 1104 90-91 0.5938444982949633 1201 1 0.21148038863272234 1201 2 0.19589813557808355 1201 3 0.23311074326893788 1201 4 0.19984222381447125 1201 5 0.12657277534971456 1201 6 -0.15229948268432736 1201 7 0.11886430636774747 1201 8 0.2059805764271161 1201 9 0.026124238237322572 1201 10-11 -0.07942515435627229 1201 12-13 -0.004555719483789744 1201 14-15 0.061760420394755045 1201 16-17 -0.056041353126516924 1201 18-19 0.10036294502794618 1201 20-21 -0.06499086913718344 1201 22-23 -0.10922036405608537 1201 24-25 -0.03110344821003963 1201 26-27 0.04434264116412123 1201 28-29 0.018946371841785492 1201 30-31 0.005418970389293065 1201 32-33 -0.026496245964857223 1201 34-35 0.03315579231822596 1201 36-37 0.03398691630853534 1201 38-39 -0.013997014481688552 1201 40-41 -0.028978696954247596 1201 42-43 -0.011689057269023806 1201 44-45 0.03864240132883623 1201 46-47 0.16175257951115896 1201 48-49 0.07121326461472677 1201 50-51 0.0013148016077195734 1201 52-53 0.11165907843729883 1201 54-55 0.05340675356297453 1201 56-57 -0.1389816285126031 1201 58-59 0.20052996750322905 1201 60-61 0.025787063799697307 1201 62-63 -0.0534384608056655 1201 64-65 -0.08563047575120208 1201 66-67 0.022845923349159136 1201 68-69 -0.15554413481256546 1201 70-71 0.033834655067920494 1201 72-73 -0.28206856177715167 1201 74-75 0.11220775223121393 1201 76-77 0.010207326671583417 1201 78-79 -0.39728376764605144 1201 80-81 0.11201849055766644 1201 82-83 -0.17049202264172436 1201 84-85 -0.5850558668720538 1201 86-87 -0.06428749164810199 1201 88-89 -1.2966631984715846 1201 90-91 0.06051116496163189 1202 1 0.05673083283146951 1202 2 0.14527775546979882 1202 3 0.1936652950701543 1202 4 0.3413434208289772 1202 5 0.1430930736595073 1202 6 0.04056277525356933 1202 7 -0.12233396657553186 1202 8 -0.002905795585213866 1202 9 -0.06370907861646202 1202 10-11 -0.03993536118959895 1202 12-13 0.027527265531809064 1202 14-15 0.019438541846035662 1202 16-17 -3.860213931972112E-4 1202 18-19 -0.04635417902111527 1202 20-21 0.1380827165429892 1202 22-23 0.08148816665730152 1202 24-25 -0.016259869091726387 1202 26-27 0.04796574489239447 1202 28-29 -0.11978985649884066 1202 30-31 0.03350970978170409 1202 32-33 -0.009765785622356304 1202 34-35 0.005735108612974216 1202 36-37 0.15173523929195198 1202 38-39 0.03960780445210332 1202 40-41 -0.01715537348629681 1202 42-43 -0.011551433194163963 1202 44-45 -0.11996612000501017 1202 46-47 -0.09938642899778927 1202 48-49 0.1233653424266663 1202 50-51 -0.02727616248550646 1202 52-53 -0.06016515127034694 1202 54-55 -0.10323894845808468 1202 56-57 -0.05966390913767583 1202 58-59 -0.15627643071536568 1202 60-61 -0.10504361701290321 1202 62-63 0.14248958340275664 1202 64-65 -0.09146131890259568 1202 66-67 -0.07114277962222815 1202 68-69 -0.2281561887723953 1202 70-71 -0.25258169390980356 1202 72-73 -0.41678621088622947 1202 74-75 -0.10108347821667962 1202 76-77 0.13698195147518533 1202 78-79 -0.07387511864421015 1202 80-81 0.03958101388726476 1202 82-83 -0.942531747622624 1202 84-85 -0.9990400793275924 1202 86-87 -0.9495741700624123 1202 88-89 0.3868384850301041 1202 90-91 0.5938444982949633 1203 1 0.2764171873728358 1203 2 0.1491153803835772 1203 3 0.0680041712594317 1203 4 0.15797426890303967 1203 5 0.16405521616240293 1203 6 0.041705169835744016 1203 7 0.09330498201101278 1203 8 0.10262090874323349 1203 9 0.13872278529949256 1203 10-11 0.07045735119945817 1203 12-13 0.06252421572590805 1203 14-15 -0.16008728459436128 1203 16-17 0.035724820524244194 1203 18-19 0.11756120169157924 1203 20-21 0.0955872342461106 1203 22-23 0.026214282686737533 1203 24-25 0.040905464591247664 1203 26-27 0.017481367309322593 1203 28-29 0.05889684577739729 1203 30-31 -0.03413099458094848 1203 32-33 -5.725750460356949E-4 1203 34-35 0.043825351660352396 1203 36-37 0.10576474614916265 1203 38-39 0.0174388323762642 1203 40-41 0.06747273289569478 1203 42-43 0.04704073921762131 1203 44-45 0.01801148669145647 1203 46-47 -0.006773403309161097 1203 48-49 0.028042350869228017 1203 50-51 0.12954544824086156 1203 52-53 0.03676564867001275 1203 54-55 -0.046720252107448346 1203 56-57 0.10684998764561016 1203 58-59 0.058435833379803626 1203 60-61 0.056562401785924976 1203 62-63 0.11202603531009458 1203 64-65 -0.111542302675808 1203 66-67 -0.4573104379217554 1203 68-69 -0.3285968278232474 1203 70-71 -0.18115783552626397 1203 72-73 -0.09057510769068244 1203 74-75 -0.0351529641696402 1203 76-77 0.21926925041455547 1203 78-79 0.47303096439073045 1203 80-81 0.19917587733454667 1203 82-83 0.21306794680059227 1203 84-85 -0.11385951181808451 1203 86-87 -0.09935064926992965 1203 88-89 0.45417855237016624 1203 90-91 -0.02154011708964987 1204 1 0.2836474607227615 1204 2 0.10586618673275439 1204 3 0.03360934839570007 1204 4 -0.06738227802262742 1204 5 -0.19176759654420295 1204 6 -0.11368925460300972 1204 7 0.04160961318564915 1204 8 -0.11560089997587397 1204 9 0.02085630217609946 1204 10-11 -0.04422607464736217 1204 12-13 -0.0665589617295339 1204 14-15 -0.07798025700750344 1204 16-17 -0.17900018482246338 1204 18-19 -0.06587604420393944 1204 20-21 -0.14041388177806624 1204 22-23 -0.1613005768277489 1204 24-25 -0.08314257655834467 1204 26-27 -0.15737617133627424 1204 28-29 -0.18136644039269356 1204 30-31 -0.2315673958589457 1204 32-33 -0.15207288201095537 1204 34-35 -0.05186363300147434 1204 36-37 -0.13685968602651855 1204 38-39 -0.14908131744350328 1204 40-41 -0.0410546817635975 1204 42-43 -0.0758247920167463 1204 44-45 -0.18481084400715275 1204 46-47 -0.15062399277131533 1204 48-49 -0.11331724404188037 1204 50-51 -0.12345630968631838 1204 52-53 -0.06805659183911672 1204 54-55 -0.06323527917810168 1204 56-57 -0.18745225594378212 1204 58-59 -0.17402941175012643 1204 60-61 0.10828066073061393 1204 62-63 -0.36468604953427075 1204 64-65 -0.22521817063054783 1204 66-67 -0.2612126832361952 1204 68-69 -0.027226505718324745 1204 70-71 -0.3591752424352279 1204 72-73 -0.1102575350609527 1204 74-75 -0.30755625124319863 1204 76-77 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0.010224108217769867 2401 54-55 0.022752877890468426 2401 56-57 0.08623470607085437 2401 58-59 0.11098663309797274 2401 60-61 0.12136673030877887 2401 62-63 0.056542225501416965 2401 64-65 0.0937850806677929 2401 66-67 0.14947579279000678 2401 68-69 0.13344122349063525 2401 70-71 0.0951964757361381 2401 72-73 0.3064407538743268 2401 74-75 -0.03419311654009505 2401 76-77 0.004265034233881693 2401 78-79 0.02020815901536821 2401 80-81 0.03188946863997444 2401 82-83 0.13670317867112658 2401 84-85 0.4617001489671111 2401 86-87 0.03520914697897126 2401 88-89 0.7504748486664639 2401 90-91 0.5938444982949633 2402 1 0.2711001069244787 2402 2 0.24686503736585053 2402 3 0.22634506606488713 2402 4 0.29947366602340253 2402 5 0.178949889543496 2402 6 0.262953436403933 2402 7 0.21260422309194382 2402 8 0.11305696314669689 2402 9 0.20237122209251623 2402 10-11 0.0888323398878228 2402 12-13 0.1315863404547386 2402 14-15 0.1796249962961909 2402 16-17 0.0937027646679951 2402 18-19 0.042595014312176716 2402 20-21 0.02042660901224025 2402 22-23 0.04257029717541627 2402 24-25 0.0057195502074876 2402 26-27 0.05553371144720387 2402 28-29 0.08336299769328548 2402 30-31 0.08183589962285254 2402 32-33 0.053068386438944515 2402 34-35 0.06803090596550021 2402 36-37 0.016459023512446436 2402 38-39 -0.03511725295621204 2402 40-41 0.09747150503277169 2402 42-43 0.09199221983148931 2402 44-45 0.04990496028198521 2402 46-47 0.08000783559020874 2402 48-49 0.034435827666300156 2402 50-51 0.0061293692950386 2402 52-53 0.10995083332391431 2402 54-55 0.07634228812604249 2402 56-57 0.11824860877337784 2402 58-59 0.0527825541261322 2402 60-61 0.2803176989792888 2402 62-63 0.15654190901647524 2402 64-65 0.07951507435736715 2402 66-67 0.15032902607588738 2402 68-69 0.02767823343868514 2402 70-71 0.2599453432966854 2402 72-73 0.010511865942319787 2402 74-75 0.25748433138385707 2402 76-77 0.09442170129673144 2402 78-79 0.07887284798724181 2402 80-81 0.09357184682546915 2402 82-83 0.004611314147538792 2402 84-85 0.06199252329331273 2402 86-87 0.04257786827174215 2402 88-89 0.39180038472884604 2402 90-91 0.5938444982949633 2403 1 -0.1781583922208867 2403 2 -0.26339162776109504 2403 3 -0.10639966624833619 2403 4 -0.09645407248182636 2403 5 0.027231155074666447 2403 6 0.01001611591587448 2403 7 0.040909435459319354 2403 8 0.13727860976130302 2403 9 0.030293129159815635 2403 10-11 0.040879547508708924 2403 12-13 0.06119751945636409 2403 14-15 0.016105980780118045 2403 16-17 0.02920060354074394 2403 18-19 0.02900034472460078 2403 20-21 0.008337549649944265 2403 22-23 0.044943866520860354 2403 24-25 -0.0012263965988310588 2403 26-27 0.02627744716664182 2403 28-29 0.009456213644241984 2403 30-31 0.10154071556159039 2403 32-33 -0.037551629114283 2403 34-35 0.10550042697217776 2403 36-37 0.1161026510770995 2403 38-39 -0.030378989584093574 2403 40-41 0.06079826495759022 2403 42-43 0.1381898613784287 2403 44-45 0.15052630927433341 2403 46-47 0.10853490484944928 2403 48-49 0.0856310206022215 2403 50-51 0.08662840985624598 2403 52-53 0.16723737330240596 2403 54-55 0.10788398153535184 2403 56-57 0.20847953533026953 2403 58-59 0.14985183917971767 2403 60-61 0.010215517729321277 2403 62-63 0.22287800763762533 2403 64-65 0.14840241004767307 2403 66-67 0.2772850689166404 2403 68-69 0.35330870495065625 2403 70-71 0.27204522112958784 2403 72-73 0.28227131112137727 2403 74-75 0.19890124864627978 2403 76-77 0.08128442261615731 2403 78-79 0.2413969868851069 2403 80-81 0.04904528354543913 2403 82-83 -0.13108911679309188 2403 84-85 0.4327162149679822 2403 86-87 0.4680454464819377 2403 88-89 0.23884694168972231 2403 90-91 -0.35487345042298557 2404 1 -0.28913696224777397 2404 2 -0.16618796363238175 2404 3 -0.2953292541485979 2404 4 -0.17584196684423858 2404 5 -0.15394454522699164 2404 6 -0.12573717719928368 2404 7 -0.1196379389236597 2404 8 -0.0017718184525605807 2404 9 0.043545364209954585 2404 10-11 0.04271857456660655 2404 12-13 0.08760301305793661 2404 14-15 0.0484366384695889 2404 16-17 0.02537111850422491 2404 18-19 0.051514844235256874 2404 20-21 0.04070973375684872 2404 22-23 -0.03703927281696906 2404 24-25 0.046988271874390364 2404 26-27 -0.04172814984507767 2404 28-29 -0.04900392747376969 2404 30-31 0.01366987958017063 2404 32-33 -0.029069648785103652 2404 34-35 0.10473436667568592 2404 36-37 0.026020229565247632 2404 38-39 0.008321895569039839 2404 40-41 0.020022478116885623 2404 42-43 0.07103002625451182 2404 44-45 -0.06877684886854496 2404 46-47 -0.018636572605160495 2404 48-49 -0.0593262792739182 2404 50-51 -0.0012354585086242764 2404 52-53 -0.07660767150263581 2404 54-55 0.07522527696699655 2404 56-57 0.013877236324489672 2404 58-59 -0.028640743499821042 2404 60-61 0.010551670357287435 2404 62-63 0.06611262620709368 2404 64-65 0.06338960235198954 2404 66-67 0.19819092719733078 2404 68-69 0.2342154170696844 2404 70-71 0.15533708139913216 2404 72-73 0.16821867483901798 2404 74-75 0.005235756860841434 2404 76-77 0.12939669046527058 2404 78-79 0.10627738352576444 2404 80-81 -0.05934416467178494 2404 82-83 0.13202143517268894 2404 84-85 -0.08821569365859716 2404 86-87 0.15006752439876436 2404 88-89 -0.06606261903379362 2404 90-91 0.34384449829496333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 19.0 31 359.0 32 2257.0 33 4191.0 34 5498.0 35 6893.0 36 9927.0 37 15505.0 38 41363.0 39 236826.0 40 240290.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.255866401951973 15.22742851460252 26.264468445558865 30.252236637886636 2 30.1656422433007 16.96487716687297 25.744075896718233 27.125404693108095 3 28.02638144774765 19.93969345951062 19.77987047234796 32.25405462039377 4 26.775972780611156 21.561882911167622 19.549729368811352 32.11241493940987 5 27.208556491596937 21.81582162492364 20.025642482703752 30.949979400775668 6 27.36429373073262 24.009283857311303 22.905627139833218 25.720795272122853 7 26.29579775823614 23.937896890227446 22.557038541859047 27.209266809677374 8 25.333671918285006 24.7874373144294 22.974172834595333 26.904717932690257 9 25.034983165461494 24.689768578369396 23.485601852509554 26.789646403659557 10-11 25.292651049139803 23.971903368328338 23.0222968845449 27.713148697986963 12-13 25.29194073105937 23.760228580358284 23.030199173189754 27.917631515392593 14-15 25.17962168459036 23.103095566194543 23.156014263187057 28.56126848602804 16-17 25.717510051000836 23.227134860990752 22.62611697518149 28.429238112826923 18-19 25.68714395306218 23.389087383330256 22.644496455512776 28.279272208094785 20-21 25.73491284397153 23.479031410265517 22.70434075378955 28.081714991973406 22-23 25.623304115582958 23.15459362702618 22.863274424287198 28.35882783310366 24-25 25.406213152249578 23.370974272279128 22.987935247403787 28.23487732806751 26-27 25.73216036140984 23.40453680157975 22.898523959028854 27.964778877981562 28-29 25.871737864215593 23.10265161739427 22.81772527737921 28.207885241010928 30-31 25.965371809101 23.181087680355162 22.89953385127636 27.954006659267485 32-33 25.997277052991258 23.223515128653293 22.90919511920447 27.87001269915098 34-35 25.93999353081571 22.913735915647962 23.002160860669466 28.144109692866863 36-37 25.87552720376444 23.141346328573565 23.068258141853327 27.914868325808662 38-39 26.09624050926097 23.160734701388748 22.930810013886436 27.81221477546384 40-41 26.081427213243106 22.8285890050892 23.036183658632066 28.05380012303563 42-43 26.139224639308406 23.08426216691796 22.996576949718825 27.77993624405481 44-45 26.166954396692244 23.011303860652475 22.991657096791364 27.830084645863913 46-47 26.143835216589125 22.88837992937621 22.984364202617776 27.98342065141689 48-49 26.235814776448034 23.038249513826894 22.938604117020514 27.787331592704557 50-51 26.20814468204724 23.08002078256791 22.927309968210253 27.784524567174596 52-53 26.18655130358114 22.94316526894134 22.913661228431604 27.95662219904592 54-55 26.21517938473682 23.01186047251105 23.04440616273219 27.72855398001994 56-57 26.52557955521482 22.991158823445005 22.79149951520096 27.691762106139215 58-59 26.30478377482174 22.99739911709838 22.907620158308728 27.790196949771158 60-61 26.411008853535794 23.03442764201246 22.915298260511232 27.639265243940507 62-63 26.56160004006778 22.993898010801605 22.64274842720046 27.801753521930156 64-65 26.609723431953768 22.84191696402649 22.722036469894075 27.82632313412567 66-67 26.4355810252411 23.129501353273085 22.56419666392601 27.870720957559804 68-69 26.67647922271687 22.952533770876816 22.584160765057646 27.78682624134866 70-71 26.849853250444873 22.674308428277598 22.534260821335305 27.941577499942227 72-73 26.990646167078808 22.64810235458553 22.49059240941834 27.87065906891732 74-75 26.79899958843828 22.78595624782347 22.452907841833667 27.96213632190458 76-77 26.88281983701415 22.536277984792026 22.364355175861128 28.2165470023327 78-79 26.753143711193733 22.551687870764177 22.348718989584007 28.346449428458083 80-81 27.44319231479771 22.363984851283945 21.961019767227047 28.231803066691295 82-83 27.509819090692694 21.75970007141157 22.08700309450131 28.64347774339443 84-85 27.74001699235344 21.5904033661043 21.41643403325646 29.253145608285795 86-87 28.41568696779516 20.48970211261381 21.143816847874128 29.950794071716903 88-89 27.25518333415805 21.050027215597012 21.63887376911277 30.055915681132166 90-91 23.828464885021752 21.205717837165942 25.730267246737103 29.235550031075203 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.5 9 3.0 10 2.5 11 4.0 12 6.0 13 6.5 14 8.0 15 17.0 16 28.0 17 41.0 18 64.0 19 86.0 20 120.0 21 180.5 22 254.0 23 335.0 24 444.0 25 578.0 26 741.0 27 985.5 28 1309.5 29 1632.0 30 1984.0 31 2508.0 32 3150.5 33 3803.5 34 4653.0 35 5720.0 36 6990.5 37 8288.5 38 9822.0 39 11894.5 40 14165.5 41 16598.0 42 19239.0 43 22041.5 44 24947.5 45 28092.0 46 31217.0 47 34093.5 48 36630.5 49 39029.0 50 41391.5 51 43448.0 52 44905.5 53 45713.0 54 46050.0 55 45860.5 56 45139.0 57 43934.0 58 42480.5 59 40694.0 60 38623.0 61 36292.0 62 34200.5 63 31987.0 64 29214.0 65 26623.0 66 23929.5 67 21333.5 68 18869.0 69 16251.5 70 13749.5 71 11420.0 72 9380.0 73 7456.5 74 5745.0 75 4513.5 76 3504.0 77 2589.0 78 1912.5 79 1366.5 80 905.5 81 621.5 82 454.0 83 326.5 84 228.5 85 149.0 86 95.5 87 69.5 88 49.0 89 26.5 90 14.0 91 11.5 92 9.5 93 6.5 94 4.0 95 3.0 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.001065477120654629 2 0.008346237445127927 3 8.878976005455243E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 11289.0 32-33 23311.0 34-35 24336.0 36-37 25064.0 38-39 25644.0 40-41 26107.0 42-43 26377.0 44-45 26210.0 46-47 26126.0 48-49 25657.0 50-51 25378.0 52-53 24355.0 54-55 23606.0 56-57 22602.0 58-59 21624.0 60-61 20740.0 62-63 19847.0 64-65 18681.0 66-67 17667.0 68-69 16395.0 70-71 15420.0 72-73 14299.0 74-75 13284.0 76-77 12097.0 78-79 11167.0 80-81 9950.0 82-83 9138.0 84-85 7954.0 86-87 7079.0 88-89 6270.0 90-91 5454.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.75244349360644 #Duplication Level Percentage of deduplicated Percentage of total 1 99.7606693250237 99.51370529728783 2 0.232045320379521 0.46294175418227934 3 0.006015856199243527 0.018002890667421056 4 9.841568401701638E-4 0.003926881983516821 5 2.8534155738129774E-4 0.001423175878952778 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002486113281527468 0.0 0.0 0.0 0.0 2 0.002486113281527468 0.0 0.0 0.0 0.0 3 0.002486113281527468 0.0 0.0 0.0 0.0 4 0.002486113281527468 0.0 0.0 0.0 0.0 5 0.0028412723217456776 0.0 0.0 0.0 1.7757952010910485E-4 6 0.0030188518418547824 0.0 0.0 0.0 1.7757952010910485E-4 7 0.003551590402182097 0.0 0.0 0.0 1.7757952010910485E-4 8 0.006570442244036879 0.0 0.0 0.0 1.7757952010910485E-4 9 0.007280760324473299 0.0 0.0 0.0 1.7757952010910485E-4 10-11 0.007280760324473299 0.0 0.0 0.0 1.7757952010910485E-4 12-13 0.007280760324473299 0.0 0.0 0.0 1.7757952010910485E-4 14-15 0.007369550084527851 0.0 0.0 0.0 1.7757952010910485E-4 16-17 0.0075471296046369565 0.0 0.0 0.0 1.7757952010910485E-4 18-19 0.007902288644855166 0.0 0.0 0.0 1.7757952010910485E-4 20-21 0.008257447685073376 0.0 0.0 0.0 1.7757952010910485E-4 22-23 0.008523816965237033 0.0 0.0 0.0 1.7757952010910485E-4 24-25 0.008523816965237033 0.0 0.0 0.0 1.7757952010910485E-4 26-27 0.009234135045673452 0.0 0.0 0.0 1.7757952010910485E-4 28-29 0.009855663366055319 0.0 0.0 0.0 3.551590402182097E-4 30-31 0.010743560966600844 0.0 0.0 0.0 3.551590402182097E-4 32-33 0.0110099302467645 0.0 0.0 0.0 3.551590402182097E-4 34-35 0.0110099302467645 0.0 0.0 0.0 3.551590402182097E-4 36-37 0.011187509766873605 0.0 0.0 0.0 3.551590402182097E-4 38-39 0.011897827847310026 0.0 1.7757952010910485E-4 0.0 3.551590402182097E-4 40-41 0.012252986887528235 0.0 1.7757952010910485E-4 0.0 4.439488002727621E-4 42-43 0.012252986887528235 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 44-45 0.012341776647582788 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 46-47 0.012608145927746445 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 48-49 0.012785725447855549 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 50-51 0.012874515207910102 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 52-53 0.013318464008182863 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 54-55 0.013584833288346521 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 56-57 0.013673623048401074 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 58-59 0.013673623048401074 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 60-61 0.014206361608728388 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 62-63 0.014383941128837494 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 64-65 0.014561520648946598 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 66-67 0.014561520648946598 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 68-69 0.014739100169055703 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 70-71 0.014739100169055703 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 72-73 0.014739100169055703 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 74-75 0.014739100169055703 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 76-77 0.014739100169055703 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 78-79 0.014739100169055703 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 80 0.014739100169055703 0.0 1.7757952010910485E-4 0.0 5.327385603273145E-4 >>END_MODULE