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"TTCTC":102931, "TTCTG":84352, "TTCCA":78417, "TTCCT":103869, "TTCCC":75825, "TTCCG":63935, "TTCGA":58392, "TTCGT":49057, "TTCGC":60179, "TTCGG":68019, "TTGAA":64586, "TTGAT":59436, "TTGAC":49973, "TTGAG":62441, "TTGTA":26118, "TTGTT":66563, "TTGTC":53739, "TTGTG":48689, "TTGCA":39690, "TTGCT":61601, "TTGCC":73825, "TTGCG":43887, "TTGGA":59488, "TTGGT":65830, "TTGGC":75242, "TTGGG":73526, "TCAAA":64031, "TCAAT":46132, "TCAAC":67250, "TCAAG":70165, "TCATA":33120, "TCATT":44268, "TCATC":84809, "TCATG":48334, "TCACA":35620, "TCACT":113876, "TCACC":71858, "TCACG":38609, "TCAGA":89339, "TCAGT":29813, "TCAGC":65675, "TCAGG":58460, "TCTAA":19576, "TCTAT":33389, "TCTAC":33880, "TCTAG":29455, "TCTTA":25667, "TCTTT":86578, "TCTTC":123946, "TCTTG":73340, "TCTCA":47901, "TCTCT":67988, "TCTCC":107768, "TCTCG":60396, "TCTGA":361948, "TCTGT":40705, "TCTGC":71464, "TCTGG":82891, "TCCAA":67922, "TCCAT":58587, "TCCAC":68897, "TCCAG":266712, "TCCTA":32012, "TCCTT":92171, "TCCTC":124048, "TCCTG":102117, 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"CTCGC":58711, "CTCGG":59016, "CTGAA":362267, "CTGAT":51093, "CTGAC":42188, "CTGAG":57284, "CTGTA":22839, "CTGTT":59991, "CTGTC":86781, "CTGTG":41528, "CTGCA":59234, "CTGCT":89420, "CTGCC":82110, "CTGCG":53142, "CTGGA":84004, "CTGGT":71415, "CTGGC":81962, "CTGGG":86312, "CCAAA":106281, "CCAAT":51793, "CCAAC":104405, "CCAAG":94873, "CCATA":42830, "CCATT":50793, "CCATC":87282, "CCATG":56793, "CCACA":92409, "CCACT":43657, "CCACC":141837, "CCACG":60547, "CCAGA":159533, "CCAGT":205536, "CCAGC":123835, "CCAGG":81689, "CCTAA":25946, "CCTAT":32502, "CCTAC":34804, "CCTAG":45085, "CCTTA":31178, "CCTTT":71359, "CCTTC":103373, "CCTTG":70903, "CCTCA":76747, "CCTCT":66488, "CCTCC":136451, "CCTCG":75288, "CCTGA":69341, "CCTGT":57067, "CCTGC":102586, "CCTGG":80492, "CCCAA":108743, "CCCAT":61169, "CCCAC":101725, "CCCAG":113432, "CCCTA":35708, "CCCTT":57921, "CCCTC":75772, "CCCTG":66207, "CCCCA":114990, "CCCCT":65393, "CCCCC":81383, "CCCCG":75311, "CCCGA":70640, "CCCGT":42895, "CCCGC":85980, "CCCGG":71664, "CCGAA":82471, "CCGAT":56714, "CCGAC":59858, "CCGAG":75052, "CCGTA":30643, "CCGTT":45386, "CCGTC":59833, "CCGTG":41349, "CCGCA":72548, "CCGCT":60664, "CCGCC":135033, "CCGCG":61714, "CCGGA":72178, "CCGGT":56955, "CCGGC":93777, "CCGGG":67104, "CGAAA":75102, "CGAAT":41229, "CGAAC":55099, "CGAAG":89300, "CGATA":37410, "CGATT":47901, "CGATC":63123, "CGATG":68251, "CGACA":53378, "CGACT":56701, "CGACC":68755, "CGACG":61865, "CGAGA":101212, "CGAGT":31101, "CGAGC":54939, "CGAGG":70538, "CGTAA":21807, "CGTAT":20260, "CGTAC":18701, "CGTAG":38925, "CGTTA":19044, "CGTTT":42906, "CGTTC":49574, "CGTTG":45044, "CGTCA":49226, "CGTCT":406473, "CGTCC":48465, "CGTCG":58057, "CGTGA":37586, "CGTGT":23952, "CGTGC":32234, "CGTGG":48881, "CGCAA":51458, "CGCAT":33852, "CGCAC":40081, "CGCAG":70720, "CGCTA":25336, "CGCTT":52309, "CGCTC":54024, "CGCTG":58711, "CGCCA":90600, "CGCCT":83509, "CGCCC":83914, "CGCCG":105471, "CGCGA":50863, "CGCGT":30421, "CGCGC":55713, "CGCGG":59798, "CGGAA":694525, "CGGAT":48961, "CGGAC":38694, "CGGAG":83733, "CGGTA":31132, "CGGTT":55024, "CGGTC":54047, "CGGTG":62323, "CGGCA":78455, "CGGCT":69407, "CGGCC":82425, "CGGCG":101322, "CGGGA":63491, "CGGGT":48432, "CGGGC":61471, "CGGGG":62952, "GAAAA":108034, "GAAAT":62376, "GAAAC":79033, "GAAAG":91062, "GAATA":40912, "GAATT":45661, "GAATC":54936, "GAATG":47048, "GAACA":69696, "GAACT":302806, "GAACC":75819, "GAACG":50449, "GAAGA":756275, "GAAGT":46599, "GAAGC":91977, "GAAGG":88005, "GATAA":41514, "GATAT":36704, "GATAC":28423, "GATAG":52802, "GATTA":32176, "GATTT":61771, "GATTC":56428, "GATTG":55760, "GATCA":63925, "GATCT":57731, "GATCC":60743, "GATCG":750593, "GATGA":86564, "GATGT":43562, "GATGC":54280, "GATGG":76052, "GACAA":61733, "GACAT":38743, "GACAC":32756, "GACAG":55822, "GACTA":40261, "GACTT":42634, "GACTC":32550, "GACTG":36061, "GACCA":69835, "GACCT":52424, "GACCC":60970, "GACCG":57960, "GACGA":74025, "GACGT":29564, "GACGC":44908, "GACGG":53866, "GAGAA":105168, "GAGAT":217891, "GAGAC":57779, "GAGAG":67830, "GAGTA":23561, "GAGTT":43109, "GAGTC":28795, "GAGTG":27679, "GAGCA":587182, "GAGCT":44384, "GAGCC":59151, "GAGCG":48558, "GAGGA":120381, "GAGGT":52270, "GAGGC":76341, "GAGGG":59059, "GTAAA":21248, "GTAAT":19722, "GTAAC":18515, "GTAAG":21076, "GTATA":13022, "GTATT":25530, "GTATC":26085, "GTATG":21569, "GTACA":19696, "GTACT":17973, "GTACC":21726, "GTACG":18228, "GTAGA":81171, "GTAGT":19761, "GTAGC":25407, "GTAGG":25810, "GTTAA":16790, "GTTAT":23969, "GTTAC":19317, "GTTAG":28894, "GTTTA":23740, "GTTTT":68045, "GTTTC":70934, "GTTTG":64067, "GTTCA":53328, "GTTCT":61120, "GTTCC":67581, "GTTCG":47497, "GTTGA":59821, "GTTGT":54578, "GTTGC":52378, "GTTGG":75642, "GTCAA":49247, "GTCAT":39932, "GTCAC":131710, "GTCAG":48934, "GTCTA":24450, "GTCTT":57308, "GTCTC":53111, "GTCTG":381580, "GTCCA":46446, "GTCCT":49548, "GTCCC":42616, "GTCCG":35312, "GTCGA":82688, "GTCGT":41699, "GTCGC":46308, "GTCGG":50451, "GTGAA":48312, "GTGAT":43012, "GTGAC":29512, "GTGAG":40826, "GTGTA":16698, "GTGTT":38312, "GTGTC":25681, "GTGTG":33678, "GTGCA":31154, "GTGCT":42799, "GTGCC":42677, "GTGCG":32738, "GTGGA":59627, "GTGGT":63774, "GTGGC":58397, "GTGGG":64362, "GCAAA":66714, "GCAAT":40770, "GCAAC":60003, "GCAAG":69409, "GCATA":30011, "GCATT":36831, "GCATC":54277, "GCATG":37483, "GCACA":519001, "GCACT":26343, "GCACC":67478, "GCACG":35042, "GCAGA":135697, "GCAGT":32935, "GCAGC":72179, "GCAGG":94846, "GCTAA":18913, "GCTAT":27000, "GCTAC":27939, "GCTAG":37069, "GCTTA":29321, "GCTTT":64080, "GCTTC":94687, "GCTTG":62565, "GCTCA":51513, "GCTCT":49024, "GCTCC":71509, "GCTCG":53175, "GCTGA":67633, "GCTGT":44423, "GCTGC":68992, "GCTGG":106041, "GCCAA":89076, "GCCAT":60384, "GCCAC":71482, "GCCAG":108813, "GCCTA":37958, "GCCTT":69714, "GCCTC":82912, "GCCTG":78534, "GCCCA":85259, "GCCCT":59173, "GCCCC":73521, "GCCCG":66809, "GCCGA":86726, "GCCGT":57196, "GCCGC":96468, "GCCGG":88777, "GCGAA":61096, "GCGAT":53628, "GCGAC":50909, "GCGAG":64122, "GCGTA":22224, "GCGTT":38691, "GCGTC":39484, "GCGTG":34760, "GCGCA":48638, "GCGCT":46879, "GCGCC":70688, "GCGCG":52775, "GCGGA":71561, "GCGGT":57569, "GCGGC":91943, "GCGGG":71433, "GGAAA":90039, "GGAAT":55748, "GGAAC":66613, "GGAAG":707079, "GGATA":47247, "GGATT":61175, "GGATC":57550, "GGATG":70936, "GGACA":49632, "GGACT":33698, "GGACC":46574, "GGACG":47955, "GGAGA":168787, "GGAGT":36427, "GGAGC":67664, "GGAGG":116958, "GGTAA":24178, "GGTAT":26596, "GGTAC":21031, "GGTAG":48427, "GGTTA":29162, "GGTTT":83076, "GGTTC":64744, "GGTTG":88840, "GGTCA":51280, "GGTCT":56915, "GGTCC":42668, "GGTCG":55248, "GGTGA":63996, "GGTGT":43576, "GGTGC":52941, "GGTGG":96861, "GGCAA":75555, "GGCAT":51563, "GGCAC":45244, "GGCAG":100413, "GGCTA":37330, "GGCTT":77475, "GGCTC":56444, "GGCTG":90711, "GGCCA":79249, "GGCCT":64538, "GGCCC":57017, "GGCCG":78084, "GGCGA":93674, "GGCGT":48778, "GGCGC":69186, "GGCGG":116634, "GGGAA":67863, "GGGAT":57953, "GGGAC":40270, "GGGAG":87727, "GGGTA":27779, "GGGTT":75875, "GGGTC":47826, "GGGTG":62539, "GGGCA":65217, "GGGCT":59279, "GGGCC":52312, "GGGCG":69252, "GGGGA":70116, "GGGGT":62150, "GGGGC":61427, "GGGGG":139186 }, "overrepresented_sequences": { } }, "read2_before_filtering": { "total_reads": 1185823, "total_bases": 72335203, "q20_bases": 69853253, "q30_bases": 66456659, "total_cycles": 61, "quality_curves": { "A":[39.3728,39.197,39.1768,39.0712,39.018,39.1588,39.1093,38.9472,39.14,39.1368,39.1395,39.1284,39.1489,39.1533,39.1166,39.1314,39.1419,39.1224,39.159,39.1115,38.5558,39.0799,39.1172,39.1297,38.5959,39.0897,39.1211,38.5482,39.0056,38.7592,39.1002,39.1149,39.0615,39.1008,39.048,38.5686,38.7704,38.9727,38.6475,38.7874,38.2265,38.4622,38.3453,38.146,37.8878,37.4922,36.9788,36.6432,36.7929,36.4058,36.0958,35.7847,35.5359,35.0146,34.8298,34.5509,34.2038,33.8568,33.5866,32.8907,32.5061], 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"GC":[0.584768,0.572759,0.60454,0.588208,0.581452,0.52908,0.535316,0.521436,0.517626,0.529522,0.530867,0.525978,0.535686,0.538559,0.535899,0.540747,0.54175,0.53675,0.541571,0.541149,0.535575,0.540315,0.54061,0.534776,0.540073,0.539035,0.5351,0.539977,0.540543,0.536543,0.539558,0.540564,0.536268,0.539369,0.54056,0.535642,0.540467,0.541453,0.536983,0.540048,0.541135,0.537698,0.540415,0.540551,0.539189,0.540617,0.542093,0.537744,0.541149,0.540513,0.537276,0.539548,0.541351,0.53736,0.541563,0.544119,0.539988,0.539413,0.540576,0.538961,0.540702,0.545662,0.541872,0.541776,0.545483,0.543262,0.544153,0.543661,0.543298,0.545364,0.548012,0.545769,0.549794,0.548732,0.54408,0.551266,0.548378,0.549449,0.552274,0.558138,0.553528,0.556503,0.565315,0.564875,0.570543,0.58052,0.583028,0.582251,0.559448,0.538293,0.527188] }, "kmer_count": { "AAAAA":95298, "AAAAT":56866, "AAAAC":79036, "AAAAG":74471, "AAATA":35455, "AAATT":36359, "AAATC":58524, "AAATG":40741, "AAACA":64396, "AAACT":41718, "AAACC":102128, 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"CTTGT":47849, "CTTGC":53732, "CTTGG":64345, "CTCAA":57732, "CTCAT":45204, "CTCAC":37489, "CTCAG":45120, "CTCTA":24526, "CTCTT":65431, "CTCTC":55604, "CTCTG":49696, "CTCCA":77783, "CTCCT":111795, "CTCCC":78483, "CTCCG":63176, "CTCGA":53505, "CTCGT":43046, "CTCGC":52955, "CTCGG":53163, "CTGAA":58249, "CTGAT":46416, "CTGAC":38357, "CTGAG":42739, "CTGTA":17640, "CTGTT":55213, "CTGTC":35777, "CTGTG":38350, "CTGCA":49857, "CTGCT":81680, "CTGCC":75001, "CTGCG":47962, "CTGGA":71755, "CTGGT":65151, "CTGGC":74689, "CTGGG":70840, "CCAAA":92957, "CCAAT":46661, "CCAAC":95139, "CCAAG":69865, "CCATA":34040, "CCATT":46380, "CCATC":79335, "CCATG":51814, "CCACA":78997, "CCACT":39891, "CCACC":127254, "CCACG":54260, "CCAGA":73143, "CCAGT":48659, "CCAGC":112564, "CCAGG":73948, "CCTAA":22181, "CCTAT":29673, "CCTAC":31496, "CCTAG":22568, "CCTTA":22637, "CCTTT":65892, "CCTTC":94288, "CCTTG":65540, "CCTCA":66142, "CCTCT":60408, "CCTCC":122130, "CCTCG":68411, "CCTGA":59584, "CCTGT":52356, "CCTGC":93052, "CCTGG":73582, "CCCAA":94237, "CCCAT":55975, "CCCAC":91728, "CCCAG":82275, "CCCTA":26984, "CCCTT":53053, "CCCTC":68206, "CCCTG":60729, "CCCCA":97084, "CCCCT":58888, "CCCCC":69942, "CCCCG":67707, "CCCGA":59234, "CCCGT":38114, "CCCGC":76830, "CCCGG":64336, "CCGAA":68619, "CCGAT":50284, "CCGAC":53281, "CCGAG":54622, "CCGTA":23388, "CCGTT":41091, "CCGTC":53206, "CCGTG":37374, "CCGCA":59646, "CCGCT":54756, "CCGCC":118944, "CCGCG":54415, "CCGGA":59700, "CCGGT":50284, "CCGGC":82967, "CCGGG":59318, "CGAAA":64480, "CGAAT":36643, "CGAAC":47569, "CGAAG":66864, "CGATA":29654, "CGATT":43025, "CGATC":54668, "CGATG":61718, "CGACA":43680, "CGACT":33812, "CGACC":61353, "CGACG":55305, "CGAGA":55337, "CGAGT":27873, "CGAGC":48119, "CGAGG":63311, "CGTAA":18566, "CGTAT":18456, "CGTAC":17259, "CGTAG":22615, "CGTTA":14473, "CGTTT":38894, "CGTTC":44952, "CGTTG":41125, "CGTCA":41585, "CGTCT":40788, "CGTCC":44519, "CGTCG":51784, "CGTGA":32703, "CGTGT":21698, "CGTGC":29123, "CGTGG":44156, "CGCAA":44089, "CGCAT":30659, "CGCAC":35337, "CGCAG":49430, "CGCTA":19034, "CGCTT":47477, "CGCTC":48251, "CGCTG":52684, "CGCCA":74789, "CGCCT":75337, "CGCCC":75026, "CGCCG":92565, "CGCGA":43034, "CGCGT":26992, "CGCGC":49701, "CGCGG":52675, "CGGAA":54777, "CGGAT":44055, "CGGAC":34343, "CGGAG":59031, "CGGTA":23604, "CGGTT":49508, "CGGTC":48042, "CGGTG":55364, "CGGCA":62743, "CGGCT":62653, "CGGCC":73546, "CGGCG":88421, "CGGGA":52822, "CGGGT":43609, "CGGGC":55000, "CGGGG":55856, "GAAAA":96330, "GAAAT":57096, "GAAAC":72962, "GAAAG":71979, "GAATA":35028, "GAATT":41155, "GAATC":49909, "GAATG":42850, "GAACA":59639, "GAACT":45705, "GAACC":68033, "GAACG":45407, "GAAGA":106600, "GAAGT":42445, "GAAGC":84218, "GAAGG":80206, "GATAA":37411, "GATAT":33582, "GATAC":26167, "GATAG":32107, "GATTA":26962, "GATTT":55724, "GATTC":51124, "GATTG":50607, "GATCA":55085, "GATCT":51817, "GATCC":54873, "GATCG":50723, "GATGA":76429, "GATGT":39981, "GATGC":49826, "GATGG":69091, "GACAA":54850, "GACAT":35296, "GACAC":29614, "GACAG":35470, "GACTA":18978, "GACTT":39453, "GACTC":29823, "GACTG":32736, "GACCA":58994, "GACCT":47498, "GACCC":55507, "GACCG":51518, "GACGA":64353, "GACGT":26985, "GACGC":40269, "GACGG":49019, "GAGAA":93006, "GAGAT":57163, "GAGAC":52667, "GAGAG":49948, "GAGTA":19158, "GAGTT":39368, "GAGTC":26600, "GAGTG":25224, "GAGCA":56947, "GAGCT":39988, "GAGCC":53091, "GAGCG":43124, "GAGGA":103478, "GAGGT":47423, "GAGGC":68821, "GAGGG":53840, "GTAAA":18975, "GTAAT":18144, "GTAAC":17028, "GTAAG":14887, "GTATA":10981, "GTATT":23457, "GTATC":23833, "GTATG":19881, "GTACA":16956, "GTACT":16616, "GTACC":19833, "GTACG":16585, "GTAGA":26151, "GTAGT":18355, "GTAGC":23680, "GTAGG":23605, "GTTAA":14637, "GTTAT":22238, "GTTAC":18157, "GTTAG":14000, "GTTTA":18591, "GTTTT":62796, "GTTTC":64867, "GTTTG":59302, "GTTCA":46324, "GTTCT":56446, "GTTCC":61507, "GTTCG":43010, "GTTGA":52936, "GTTGT":50415, "GTTGC":47756, "GTTGG":69434, "GTCAA":43091, "GTCAT":36484, "GTCAC":28608, "GTCAG":33843, "GTCTA":18171, "GTCTT":52092, "GTCTC":49884, "GTCTG":38672, "GTCCA":39866, "GTCCT":45636, "GTCCC":39335, "GTCCG":32028, "GTCGA":44162, "GTCGT":37599, "GTCGC":41438, "GTCGG":44876, "GTGAA":41589, "GTGAT":39294, "GTGAC":26799, "GTGAG":30583, "GTGTA":13153, "GTGTT":35110, "GTGTC":23364, "GTGTG":30868, "GTGCA":26057, "GTGCT":39007, "GTGCC":38849, "GTGCG":29533, "GTGGA":50691, "GTGGT":57491, "GTGGC":52859, "GTGGG":58169, "GCAAA":57386, "GCAAT":37218, "GCAAC":54564, "GCAAG":51367, "GCATA":23879, "GCATT":33851, "GCATC":49058, "GCATG":34405, "GCACA":36488, "GCACT":23854, "GCACC":60396, "GCACG":31032, "GCAGA":60099, "GCAGT":30282, "GCAGC":65221, "GCAGG":84742, "GCTAA":15962, "GCTAT":24777, "GCTAC":25840, "GCTAG":17445, "GCTTA":22512, "GCTTT":58909, "GCTTC":87201, "GCTTG":57619, "GCTCA":44075, "GCTCT":44560, "GCTCC":65075, "GCTCG":47348, "GCTGA":58102, "GCTGT":41037, "GCTGC":62860, "GCTGG":96279, "GCCAA":76470, "GCCAT":55154, "GCCAC":64652, "GCCAG":75137, "GCCTA":28785, "GCCTT":64025, "GCCTC":75902, "GCCTG":70878, "GCCCA":71069, "GCCCT":54221, "GCCCC":66187, "GCCCG":59292, "GCCGA":72455, "GCCGT":51809, "GCCGC":85294, "GCCGG":77796, "GCGAA":50514, "GCGAT":48046, "GCGAC":45705, "GCGAG":47234, "GCGTA":16955, "GCGTT":35214, "GCGTC":35649, "GCGTG":31456, "GCGCA":39214, "GCGCT":42351, "GCGCC":63356, "GCGCG":46771, "GCGGA":58597, "GCGGT":51389, "GCGGC":80995, "GCGGG":63160, "GGAAA":77847, "GGAAT":50676, "GGAAC":60142, "GGAAG":75326, "GGATA":38089, "GGATT":55819, "GGATC":52050, "GGATG":64318, "GGACA":41002, "GGACT":30883, "GGACC":42056, "GGACG":42634, "GGAGA":96839, "GGAGT":33329, "GGAGC":60459, "GGAGG":103062, "GGTAA":20720, "GGTAT":24397, "GGTAC":19314, "GGTAG":27567, "GGTTA":22017, "GGTTT":76315, "GGTTC":59226, "GGTTG":80637, "GGTCA":42913, "GGTCT":51903, "GGTCC":38780, "GGTCG":48464, "GGTGA":54939, "GGTGT":39455, "GGTGC":48037, "GGTGG":85808, "GGCAA":64094, "GGCAT":47328, "GGCAC":41188, "GGCAG":66529, "GGCTA":28329, "GGCTT":71400, "GGCTC":51282, "GGCTG":82564, "GGCCA":64658, "GGCCT":58958, "GGCCC":51418, "GGCCG":68695, "GGCGA":78977, "GGCGT":44239, "GGCGC":61286, "GGCGG":101898, "GGGAA":57321, "GGGAT":52704, "GGGAC":36747, "GGGAG":64991, "GGGTA":21451, "GGGTT":69236, "GGGTC":43581, "GGGTG":55408, "GGGCA":53037, "GGGCT":54430, "GGGCC":47157, "GGGCG":61503, "GGGGA":59122, "GGGGT":54353, "GGGGC":55230, "GGGGG":48052 }, "overrepresented_sequences": { } }, "command": "/redser4/software/fastp -l 30 -q 25 -y -Y 30 --detect_adapter_for_pe -i /scratch3/cwanket/andy-lake/raw/AM-AL-6B_S63_R1_001.fastq.gz -I /scratch3/cwanket/andy-lake/raw/AM-AL-6B_S63_R2_001.fastq.gz -o AM-AL-6B.trim.R1.fastq.gz -O AM-AL-6B.trim.R2.fastq.gz -m --merged_out AM-AL-6B.trim.merged.fastq.gz " }