##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-6A_S53_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1502461
Sequences flagged as poor quality	0
Sequence length	61
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.3439377128591	40.0	40.0	40.0	40.0	40.0
2	39.284408713437486	40.0	40.0	40.0	40.0	40.0
3	39.22385206670922	40.0	40.0	40.0	40.0	40.0
4	39.08606479635744	40.0	40.0	40.0	40.0	40.0
5	39.081267334060584	40.0	40.0	40.0	40.0	40.0
6	39.21632042362497	40.0	40.0	40.0	40.0	40.0
7	39.22834336465306	40.0	40.0	40.0	40.0	40.0
8	39.23531060040826	40.0	40.0	40.0	40.0	40.0
9	39.27212486713466	40.0	40.0	40.0	40.0	40.0
10	39.29173269722143	40.0	40.0	40.0	40.0	40.0
11	39.27184000117141	40.0	40.0	40.0	40.0	40.0
12	39.28193810022356	40.0	40.0	40.0	40.0	40.0
13	39.17989485251198	40.0	40.0	40.0	40.0	40.0
14	39.30481523314083	40.0	40.0	40.0	40.0	40.0
15	39.25694710212112	40.0	40.0	40.0	40.0	40.0
16	39.2749389168837	40.0	40.0	40.0	40.0	40.0
17	39.298228706102854	40.0	40.0	40.0	40.0	40.0
18	39.26118548168638	40.0	40.0	40.0	40.0	40.0
19	39.314713659788836	40.0	40.0	40.0	40.0	40.0
20	38.85684087640212	40.0	40.0	40.0	40.0	40.0
21	39.112317724054066	40.0	40.0	40.0	40.0	40.0
22	39.11610351283661	40.0	40.0	40.0	40.0	40.0
23	39.257740467140245	40.0	40.0	40.0	40.0	40.0
24	39.28812262015453	40.0	40.0	40.0	40.0	40.0
25	38.97479402127576	40.0	40.0	40.0	40.0	40.0
26	39.232563108127266	40.0	40.0	40.0	40.0	40.0
27	39.24707796075905	40.0	40.0	40.0	40.0	40.0
28	38.88161356600937	40.0	40.0	40.0	40.0	40.0
29	39.18447933091109	40.0	40.0	40.0	40.0	40.0
30	38.68978695620053	40.0	40.0	40.0	40.0	40.0
31	38.93532810502236	40.0	40.0	40.0	40.0	40.0
32	39.0300100967679	40.0	40.0	40.0	40.0	40.0
33	39.066023011579006	40.0	40.0	40.0	40.0	40.0
34	38.90171125906097	40.0	40.0	40.0	40.0	40.0
35	38.660057066373106	40.0	40.0	40.0	40.0	40.0
36	38.88541799088296	40.0	40.0	40.0	40.0	40.0
37	38.82798422055548	40.0	40.0	40.0	40.0	40.0
38	38.530083642770094	40.0	40.0	40.0	40.0	40.0
39	38.65858215288117	40.0	40.0	40.0	40.0	40.0
40	38.27601248884331	40.0	40.0	40.0	40.0	40.0
41	38.572635163242175	40.0	40.0	40.0	40.0	40.0
42	38.02116926828716	40.0	40.0	40.0	40.0	40.0
43	38.299903957573605	40.0	40.0	40.0	40.0	40.0
44	37.838429084016155	40.0	40.0	40.0	24.0	40.0
45	38.13480948923134	40.0	40.0	40.0	40.0	40.0
46	37.69032540611703	40.0	40.0	40.0	24.0	40.0
47	37.03685619793126	40.0	40.0	40.0	24.0	40.0
48	37.39405947974689	40.0	40.0	40.0	24.0	40.0
49	36.92843142018329	40.0	40.0	40.0	24.0	40.0
50	37.21505716288143	40.0	40.0	40.0	24.0	40.0
51	36.669035668812704	40.0	40.0	40.0	24.0	40.0
52	36.72625113064499	40.0	40.0	40.0	24.0	40.0
53	35.89090166067538	40.0	40.0	40.0	24.0	40.0
54	35.07940106265654	40.0	40.0	40.0	12.0	40.0
55	35.06126548376297	40.0	40.0	40.0	12.0	40.0
56	34.75813082669034	40.0	40.0	40.0	12.0	40.0
57	34.79706428319936	40.0	40.0	40.0	12.0	40.0
58	34.77417383878849	40.0	40.0	40.0	12.0	40.0
59	34.30613906118029	40.0	24.0	40.0	12.0	40.0
60	33.576112790947654	40.0	24.0	40.0	12.0	40.0
61	33.401718913169795	40.0	24.0	40.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.10069937584006539
1101	2	-0.04386690589086584
1101	3	-0.001280447025997944
1101	4	0.03680927424110081
1101	5	-0.05357471741184838
1101	6	-0.061499235718500245
1101	7	-0.042308916414832254
1101	8	0.08674868245719125
1101	9	-0.058149512880568466
1101	10	0.054249947296476364
1101	11	0.0070278583643670345
1101	12	0.036781365390993415
1101	13	-0.11021854780369722
1101	14	-0.09078216404661532
1101	15	-0.08674733283946523
1101	16	0.031732123289380354
1101	17	-0.06960321940595549
1101	18	-0.007017410857955042
1101	19	0.007165143113603278
1101	20	0.06355488096240691
1101	21	0.012648095557054262
1101	22	0.003057415689902143
1101	23	-0.007389235956722473
1101	24	-0.047193702843451035
1101	25	0.028937397647247565
1101	26	0.025577428471805774
1101	27	0.052171126886619845
1101	28	0.06653167880689637
1101	29	-0.022208853801558348
1101	30	0.06982772543945259
1101	31	-0.057472522244836455
1101	32	0.06576383602786962
1101	33	0.0389697108431335
1101	34	0.05780222786642497
1101	35	0.09013602407943466
1101	36	-0.00581343056579442
1101	37	0.06172593963388806
1101	38	-0.024951750836763154
1101	39	0.010828021972209001
1101	40	-0.01708897848696722
1101	41	0.16851122566712462
1101	42	0.08264418001871121
1101	43	0.14004291781546385
1101	44	0.10289292007709605
1101	45	0.0858943802981571
1101	46	0.03234074673522258
1101	47	-0.09427368586860752
1101	48	0.2262267042282815
1101	49	0.12773414220828272
1101	50	0.07472755760957028
1101	51	0.356482869831801
1101	52	0.15849397036348023
1101	53	0.0679864682622977
1101	54	0.2098206023078646
1101	55	0.07026864323902515
1101	56	0.30902628785852926
1101	57	0.07346876235422428
1101	58	0.3557757104300521
1101	59	0.023053978303373412
1101	60	0.2741196479657404
1101	61	0.2772278203387657
1102	1	-0.0448675459111314
1102	2	-0.03597353554473415
1102	3	0.01814864791122517
1102	4	-0.1146440152234689
1102	5	-0.04639089886477166
1102	6	0.019472024941990185
1102	7	-0.09417631363309908
1102	8	-0.040732667000867195
1102	9	-0.03218442823202139
1102	10	-0.11446111767686773
1102	11	-0.01756340950616675
1102	12	-0.0234670660392311
1102	13	-0.009262160978543932
1102	14	-0.09013398550568752
1102	15	-0.04600125958052104
1102	16	0.007386348451341007
1102	17	0.06674263166781458
1102	18	-0.041267360795472996
1102	19	0.00393194392280094
1102	20	0.09708706102927778
1102	21	-0.01788413867039651
1102	22	-0.02924747406707695
1102	23	-0.04734340184802477
1102	24	0.006829102217814409
1102	25	0.005395420152787267
1102	26	0.06131939553630872
1102	27	-0.10498602882655916
1102	28	0.030662943616640348
1102	29	-0.041455227785100135
1102	30	0.030304221470544235
1102	31	-0.04208042384199473
1102	32	0.019586841409001465
1102	33	0.19580513779578013
1102	34	0.04740496847090725
1102	35	0.0637936075476162
1102	36	0.07607982778725386
1102	37	0.04467886149274847
1102	38	0.05040009737475515
1102	39	0.14348640683351022
1102	40	0.11383679208729802
1102	41	0.07990834898362209
1102	42	0.11755629670413725
1102	43	-0.09480771195765669
1102	44	0.06761273869550877
1102	45	0.07262289407346145
1102	46	0.09418243494044987
1102	47	-0.04457865719431453
1102	48	0.13227613601638666
1102	49	0.27802431226248103
1102	50	-0.07438917673160006
1102	51	0.010298352180370784
1102	52	0.06146914396259717
1102	53	0.12210631650948756
1102	54	0.35264351777105674
1102	55	0.15174435361372218
1102	56	-0.16157895956378354
1102	57	0.22936732914439517
1102	58	-0.2546268032857171
1102	59	-0.039087407236394256
1102	60	0.1439350775088215
1102	61	-0.003563053650090353
1103	1	-0.03632670338878796
1103	2	-0.03674326776285852
1103	3	-0.06343978211109658
1103	4	-0.035736730619312596
1103	5	-0.0743275776843575
1103	6	-0.028447256159971346
1103	7	0.04379332034242367
1103	8	0.030827125443934733
1103	9	0.022579276363508427
1103	10	-0.01516955443743484
1103	11	0.02206647493137126
1103	12	-0.020863601642616914
1103	13	0.0075546300131463795
1103	14	0.0016313661398399404
1103	15	-0.01735041082245914
1103	16	-0.11373428964428456
1103	17	0.013900249468697723
1103	18	-0.02525604590881869
1103	19	-0.04308365386008717
1103	20	0.08419484265040467
1103	21	-0.05502699176868475
1103	22	-0.044515422275949845
1103	23	-0.04478859868359564
1103	24	0.07091134430090307
1103	25	0.037146962625371316
1103	26	0.08668286839820638
1103	27	-0.03516459254517201
1103	28	-0.03697846946307237
1103	29	-2.692456174102631E-4
1103	30	-0.045948460205728736
1103	31	-0.06513912379789843
1103	32	0.008472792928969852
1103	33	0.04436731340231148
1103	34	-0.13971463340589452
1103	35	0.029616081463423427
1103	36	0.010168808123864892
1103	37	-0.01710037718369506
1103	38	-0.11775648774630554
1103	39	0.024385829198138254
1103	40	0.001900563428343105
1103	41	0.0885911053539914
1103	42	-0.002233500928838339
1103	43	0.028723338155273836
1103	44	0.2034409245552311
1103	45	0.11116896015789024
1103	46	0.09523050940664746
1103	47	0.16268191715685987
1103	48	0.3965250295484424
1103	49	0.13872153747515625
1103	50	0.12208877636817306
1103	51	0.18356949822847923
1103	52	0.175101614700381
1103	53	0.058138561034475345
1103	54	-0.16814863046486295
1103	55	0.39840601613622084
1103	56	0.41273912847093186
1103	57	0.6601680488351676
1103	58	0.43324094291711646
1103	59	0.4717817425333237
1103	60	0.4252162870536438
1103	61	0.25971226133848546
1104	1	0.032442036643111294
1104	2	0.00926417508669175
1104	3	-6.285115103494832E-4
1104	4	-7.588819572532657E-4
1104	5	0.03220759864029077
1104	6	-0.002674794703295902
1104	7	-0.05083223344102805
1104	8	-0.024212038121547153
1104	9	0.03239072417495237
1104	10	0.003047705885087737
1104	11	0.00745757895405319
1104	12	-0.046431207526012486
1104	13	-0.06941400369233008
1104	14	0.06449686321586512
1104	15	-0.03282055081891855
1104	16	0.007177636636065188
1104	17	0.035511094763748474
1104	18	0.007263181452515255
1104	19	-0.0021281337466731998
1104	20	-0.004674907482502988
1104	21	0.005209761731222784
1104	22	0.02835787207102669
1104	23	0.03572956541351857
1104	24	0.02601359487683652
1104	25	0.011466296313123792
1104	26	-0.0037041597782945246
1104	27	0.09840513986666366
1104	28	0.09979877668259007
1104	29	0.06338732003915482
1104	30	0.07643183396404396
1104	31	-0.03446040680339735
1104	32	0.025746937887326737
1104	33	0.03557659924317136
1104	34	0.02650617113069842
1104	35	0.015588082172264706
1104	36	-0.008823926237290891
1104	37	0.08850940484713732
1104	38	0.12747122800772814
1104	39	0.12067539148770123
1104	40	0.14266008850365353
1104	41	-0.03155172647304738
1104	42	0.19651680918989456
1104	43	0.08528226071419454
1104	44	0.06113647656657406
1104	45	0.15338905585166884
1104	46	0.09261877805807472
1104	47	0.09412937418326806
1104	48	-0.43050385579623196
1104	49	0.008644628029820467
1104	50	0.2135957761909566
1104	51	0.021401148060128605
1104	52	0.1274847442414071
1104	53	0.30569269890440154
1104	54	0.41918541171128965
1104	55	0.41923713433575927
1104	56	0.532825961715659
1104	57	0.4233107034515058
1104	58	0.2266040476486424
1104	59	0.18245159899264962
1104	60	0.3005893155845669
1104	61	0.17957758120380873
1201	1	0.020250495405825575
1201	2	-0.12467945483431464
1201	3	-0.006112338434050457
1201	4	-0.09145034192279411
1201	5	-0.12151917660541756
1201	6	-0.06900883239171662
1201	7	-0.023481237049487902
1201	8	-0.051653538722959524
1201	9	-0.021631099996341163
1201	10	-0.10883422253075992
1201	11	-0.083123264754974
1201	12	-0.003585092857008476
1201	13	-0.061612956912313166
1201	14	-0.004768331890495858
1201	15	-0.058143871473859576
1201	16	-0.04299342412268459
1201	17	-0.07693865221023088
1201	18	-0.02020814045943098
1201	19	-0.0511715328559319
1201	20	-0.027830288213515075
1201	21	0.017128576680889296
1201	22	0.01173786621946249
1201	23	-0.04792163795004711
1201	24	-0.12460517564427676
1201	25	-0.09047993430356627
1201	26	-0.0322660400812822
1201	27	-0.10577443817045662
1201	28	-0.009559436639470675
1201	29	0.014893187858795898
1201	30	-0.07612878933602474
1201	31	-0.044654266994328395
1201	32	-0.04294575740866691
1201	33	-0.04453852488586563
1201	34	0.035020155288130184
1201	35	0.01607527916412721
1201	36	-0.039179939804249386
1201	37	-0.048485881391933106
1201	38	0.00955221800588646
1201	39	-0.002422817283147083
1201	40	-0.07425891066195334
1201	41	-0.08093356278092756
1201	42	-0.060985526018107805
1201	43	0.008879542987202171
1201	44	0.06820759475735372
1201	45	-0.030201507957485774
1201	46	0.054732363442163035
1201	47	0.7101989861377973
1201	48	0.2907659772174327
1201	49	0.047292526342623376
1201	50	-0.05076650071811173
1201	51	-0.03163900017953125
1201	52	-0.11057739071302564
1201	53	-0.19094793913293984
1201	54	0.0010037747900781824
1201	55	0.00870865129988374
1201	56	0.3500143929543498
1201	57	0.44775308178770956
1201	58	0.12689458870170967
1201	59	0.062002978986797075
1201	60	0.2606807733828589
1201	61	0.3016282297522963
1202	1	-0.27854209034101984
1202	2	-0.235080018731189
1202	3	-0.10904622245437423
1202	4	-0.16410056950947194
1202	5	-0.09871303628034411
1202	6	-0.04004822828726162
1202	7	-0.10773448908012284
1202	8	-0.12584612333457557
1202	9	-0.05655163171476829
1202	10	-0.04473842611157153
1202	11	-0.04788745028571384
1202	12	-0.04466679649844707
1202	13	-0.13429620920611285
1202	14	-0.048285091671353086
1202	15	-0.042685212473053014
1202	16	-0.1417145939403781
1202	17	-0.07292666630559097
1202	18	-0.06042604160513321
1202	19	-0.01586915296137903
1202	20	-0.12750865852677862
1202	21	0.0021487759333993495
1202	22	-0.03254104188052054
1202	23	-0.023966341019232118
1202	24	-0.04213009768790954
1202	25	-0.03476260928420061
1202	26	-0.05531986570979086
1202	27	-0.03938600466131703
1202	28	-0.1227041472940158
1202	29	-0.1261331477970984
1202	30	-0.1327310733041287
1202	31	-0.019823815324699012
1202	32	-0.09113860931401518
1202	33	0.05514552154893693
1202	34	-0.03526158158442172
1202	35	-0.010610748237091627
1202	36	0.006801804756861429
1202	37	-0.01827756257140578
1202	38	-0.004759348433374555
1202	39	-0.02840397359166502
1202	40	-0.12402662828832689
1202	41	-0.16616826782642846
1202	42	-0.11274969039239835
1202	43	-0.013003453571521106
1202	44	-0.13260120096057904
1202	45	0.09825789461524437
1202	46	0.09424952158355637
1202	47	-0.4118288547223301
1202	48	0.19831485765406143
1202	49	0.03462290706073645
1202	50	-0.10845601996609844
1202	51	-0.10352449939183828
1202	52	-0.14585861415458368
1202	53	-0.04751699198949666
1202	54	-0.33211283390274815
1202	55	-0.14233345881378057
1202	56	0.30007742370396784
1202	57	0.016747629098958328
1202	58	0.022934874716312947
1202	59	0.10372144026248975
1202	60	0.07509223956117239
1202	61	-0.2733881664987834
1203	1	-0.08490585590985233
1203	2	-0.06707563709937858
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2403	4	0.16333843853391272
2403	5	0.06421234598132486
2403	6	0.11494897851986963
2403	7	6.457416354308521E-4
2403	8	0.023775545755754024
2403	9	-0.047172155269969096
2403	10	0.08917542761786734
2403	11	0.0260868755259267
2403	12	0.0777424356017562
2403	13	0.1098810161643371
2403	14	0.08896392853744572
2403	15	0.07548966965855897
2403	16	0.04567930277017496
2403	17	-0.010903293331381292
2403	18	0.005238988272253664
2403	19	-0.06179344620387894
2403	20	-0.015803405222946765
2403	21	-0.04533043090126654
2403	22	-0.0622148215885332
2403	23	0.011648860615963486
2403	24	0.07947559332482257
2403	25	0.04260629722607945
2403	26	-0.002147762279783194
2403	27	0.11486490158666385
2403	28	0.08930824854090247
2403	29	-0.031100104071860812
2403	30	-0.010508778625542448
2403	31	0.08938676533336576
2403	32	0.12288155239410514
2403	33	0.03766469893733415
2403	34	-0.1231453557785116
2403	35	0.03128352198602613
2403	36	-0.03559966002100623
2403	37	-0.0649640967733518
2403	38	-0.12421453453392672
2403	39	-0.047539699579083106
2403	40	0.07530517911396117
2403	41	0.04793811695599004
2403	42	0.029093063355226434
2403	43	0.07335511414004259
2403	44	-0.015168996569869364
2403	45	0.07781722949479786
2403	46	-0.07484769384588219
2403	47	0.010530889680595124
2403	48	-0.21993298803707262
2403	49	-0.20095157409735265
2403	50	0.09218362482381792
2403	51	0.07667955222317602
2403	52	0.040537558410392194
2403	53	-0.03992066668650551
2403	54	-0.018849189101239006
2403	55	-0.16136267394469428
2403	56	-0.2687717011318327
2403	57	-0.43156381154369683
2403	58	-0.249059073728084
2403	59	-0.3422409650279832
2403	60	-0.39231797432277205
2403	61	-0.2998673450754623
2404	1	-0.08060598090828819
2404	2	0.0226104759484258
2404	3	0.012048664885732308
2404	4	0.05646229733390129
2404	5	0.02366603846568438
2404	6	0.07437620839533565
2404	7	-0.033580474908468716
2404	8	0.0053940958209608425
2404	9	0.037107526925275636
2404	10	0.006331807846052584
2404	11	0.009126952215311235
2404	12	0.012041368413193254
2404	13	0.02765572692988627
2404	14	0.023965797692149238
2404	15	0.06157630078581633
2404	16	-0.01870832450658355
2404	17	0.02720584517652469
2404	18	0.03337097780200793
2404	19	0.042089811201648786
2404	20	0.014483982436750864
2404	21	-0.11344891331909679
2404	22	0.03826482251546537
2404	23	-0.012858496921090534
2404	24	0.03955097076175207
2404	25	0.003374920473440568
2404	26	-0.007339651601967034
2404	27	0.03281031481520813
2404	28	-0.08104721883908894
2404	29	-0.001974089501025844
2404	30	-0.08204354658014523
2404	31	-0.004874538393650596
2404	32	0.04039312792193073
2404	33	0.012988129788489289
2404	34	-0.05207126084631852
2404	35	-0.06170683108853581
2404	36	-0.13262425645690712
2404	37	-0.12782732869277424
2404	38	-0.05313447619691658
2404	39	-0.059113177485222934
2404	40	0.042678273200465355
2404	41	0.03202812560665791
2404	42	-0.023906324277639612
2404	43	-0.06751877065666889
2404	44	-0.07188358540614814
2404	45	0.09456545193427246
2404	46	-0.04054097133690249
2404	47	-0.06499414152478522
2404	48	-0.4094425389889622
2404	49	-0.04679644133891259
2404	50	0.053815078206206124
2404	51	-0.03660314931847353
2404	52	-0.1967992446306468
2404	53	0.006188752101429884
2404	54	-0.08950696383436707
2404	55	-0.0587399312331911
2404	56	-0.2855396773497745
2404	57	-0.4314836782914213
2404	58	-0.12514697580599687
2404	59	-0.1619936999013376
2404	60	-0.15469940040394192
2404	61	-0.020336471224212005
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	6.0
14	29.0
15	123.0
16	469.0
17	917.0
18	1338.0
19	1512.0
20	1687.0
21	1771.0
22	2072.0
23	2537.0
24	3226.0
25	3697.0
26	4475.0
27	6088.0
28	9592.0
29	11818.0
30	14187.0
31	17511.0
32	23603.0
33	27742.0
34	35646.0
35	54746.0
36	74223.0
37	101459.0
38	188357.0
39	516263.0
40	397367.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.53493552816454	32.56185442470543	18.847620435837996	20.05558961129204
2	27.986616624325023	19.652357032894695	23.37358507142615	28.98744127135413
3	29.204751404528967	20.667491535554	19.595650070118293	30.532106989798734
4	29.442627795330463	20.524526094188136	21.694473267525748	28.338372842955657
5	27.01541005057702	21.235160180530478	22.117579091903217	29.631850676989284
6	27.02905433152674	21.17359452258661	23.62770148443121	28.169649661455438
7	28.133043054029354	23.16379593214067	23.8669755820617	24.83618543176828
8	25.495769940118247	25.92932528697916	25.123713693733148	23.45119107916944
9	25.24038893522028	25.088371678199966	25.24997321061911	24.42126617596064
10	27.92751359269891	24.03643089571044	24.02784498233232	24.00821052925833
11	25.952553843327713	25.506219462601692	24.373344798966496	24.1678818951041
12	27.429330944363944	23.12346210650393	24.39184777508368	25.055359174048448
13	27.022997601934428	23.843014893564625	23.30043841404203	25.833549090458916
14	28.002057956912026	23.96201964643342	23.61166113463178	24.424261262022775
15	26.557694342814887	23.35128831963026	24.915189146340573	25.175828191214283
16	27.37249086665145	23.888007741964685	22.806116098853813	25.933385292530055
17	28.17231196017734	24.04674730325779	23.182498580661996	24.598442155902884
18	26.56202057823797	23.455583872060572	24.694750812167506	25.287644737533952
19	28.828901382465165	23.931602883535746	22.630670613080806	24.60882512091828
20	26.56727861821372	24.190045531963893	24.686164898789386	24.556510951033005
21	26.694869284460626	25.093296930835475	23.093045343606256	25.11878844109764
22	28.919020194201376	24.333010973329756	22.431464111214865	24.316504721253995
23	28.378174208848016	24.609823482938992	22.845850907278127	24.166151400934865
24	28.386227662481755	23.988975420992624	22.8890467040409	24.735750212484717
25	27.712599528373783	26.08387172778528	22.134418131319215	24.069110612521722
26	27.206629656277272	26.418655792063824	22.46008382247526	23.914630729183653
27	27.143599734036357	25.97438469284727	22.519386526505514	24.36262904661086
28	27.957464453320252	26.531870045212486	21.78678847570752	23.723877025759737
29	27.545606841042797	26.795703848552478	22.168761784831688	23.489927525573044
30	27.695427701617547	26.23755292150678	22.01235173492024	24.054667641955433
31	28.359937462603025	25.58449104502546	22.763519319303462	23.292052173068054
32	29.47344390303642	25.771384415302627	21.683624400234017	23.071547281426938
33	28.14847206331521	25.352440778434705	22.95442580157356	23.544661356676524
34	28.939253664487797	25.915880678433584	20.89871217955075	24.24615347752787
35	29.744266240521384	26.043205114808305	21.446679813985188	22.765848830685123
36	30.288506656745167	25.393005209453023	21.190766349342844	23.127721784458966
37	29.653149066764463	25.91428329920045	21.952050668869276	22.480516965165815
38	29.554178111777947	26.193092532851104	20.822037976360118	23.430691379010838
39	29.784666623626173	26.815072071754276	20.74316737672392	22.657093927895634
40	30.556866367912377	25.68392790228831	20.250575555704938	23.50863017409437
41	30.404250093679636	25.795944121012127	20.605659647737944	23.194146137570293
42	30.527847311843704	25.58142940149528	21.4322368434189	22.45848644324212
43	31.459185962231302	25.724661072733333	21.51769663239179	21.298456332643575
44	30.884928127918126	26.03555100598285	21.971751679411312	21.107769186687708
45	31.660522303074757	25.585223177173983	21.510042523566337	21.244211996184927
46	32.40217216952719	25.36418582578849	19.4481587209252	22.785483283759113
47	32.417946289454434	25.49563682518215	19.541871635935976	22.54454524942744
48	32.36190490135851	27.42147716313435	19.566897243921805	20.649720691585337
49	33.337770497869826	26.024369351350884	18.941057371871885	21.696802778907408
50	32.480443751950965	27.295550433588627	19.548527382740716	20.675478431719693
51	32.598916045075384	27.298944864459045	19.545066394402248	20.557072696063326
52	32.777090387038335	27.907479794816638	19.194108865388188	20.121320952756843
53	33.28685403481355	26.08606812423084	18.306498471507744	22.32057936944786
54	32.33361797743835	25.77051916821801	18.240673135608844	23.655189718734796
55	33.48133495644812	26.1984171302949	19.62280551708164	20.69744239617534
56	31.74391881053818	27.280841233150145	21.86931973608633	19.10592022022535
57	33.41291387929537	28.121728284461295	19.82973268524108	18.635625151002255
58	31.890145567838367	28.94797269280201	20.81677993638437	18.345101802975254
59	31.363276650774964	29.296933497774653	19.59571662758634	19.74407322386405
60	30.77790371929787	26.435627946415913	23.824179130107204	18.96228920417901
61	31.329066112198582	28.377175846827306	20.35314061396602	19.94061742700809
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	0.5
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	2.0
9	1.5
10	1.0
11	5.0
12	7.0
13	5.0
14	11.5
15	18.0
16	26.0
17	39.5
18	45.0
19	66.5
20	128.5
21	169.0
22	253.0
23	337.0
24	410.5
25	660.5
26	837.0
27	1107.0
28	1377.0
29	1808.0
30	2998.0
31	3757.0
32	4653.0
33	5549.0
34	7021.0
35	10713.5
36	12934.0
37	15785.5
38	18637.0
39	22870.5
40	32388.0
41	37672.0
42	44064.0
43	58954.0
44	67452.0
45	75283.5
46	83115.0
47	91388.0
48	107035.5
49	114410.0
50	120733.0
51	127056.0
52	130749.5
53	132326.5
54	130210.0
55	123526.5
56	116843.0
57	109756.5
58	94751.0
59	86832.0
60	79033.5
61	63846.5
62	56458.0
63	49951.0
64	43444.0
65	37798.5
66	27622.0
67	23091.0
68	19492.0
69	15893.0
70	13189.5
71	8540.0
72	6594.0
73	5316.0
74	4038.0
75	3215.0
76	1856.5
77	1321.0
78	1000.0
79	679.0
80	542.5
81	304.0
82	202.0
83	146.0
84	82.5
85	75.0
86	67.0
87	59.0
88	51.5
89	40.0
90	36.0
91	36.5
92	37.0
93	42.5
94	54.0
95	60.0
96	63.5
97	67.0
98	89.0
99	305.0
100	499.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0014642642970433175
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	6.655746804742353E-4
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1502461.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.66994700459064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.39221456417678	81.9513503660191
2	7.268066348285195	13.034542485531638
3	1.005689986415614	2.7054050335480677
4	0.20546027085916616	0.7369444639796106
5	0.05904267828471122	0.26471769163995745
6	0.02121095977304862	0.11411913832594643
7	0.013773953507618876	0.0864576776750316
8	0.008605227503459156	0.061730423535810264
9	0.005811316662034692	0.04689904114003908
>10	0.016792705870687683	0.24101780050479132
>50	0.0012728855622236477	0.08146079278340419
>100	0.001742318007247376	0.351738563362025
>500	1.5839254611339781E-4	0.08692267344137262
>1k	1.5839254611339781E-4	0.23669384851314948
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCGGTCACCTTTTCCACCCCCCTTGTCTC	2126	0.14150117706882243	Illumina Single End PCR Primer 1 (96% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.454413791772299	0.0	0.0	0.0	0.0
2	1.456610188217864	0.0	0.0	0.0	0.0
3	1.4576085502385752	0.0	0.0	0.0	0.0
4	1.4584072398551444	0.0	0.0	0.0	0.0
5	1.459938061620235	0.0	0.0	0.0	0.0
6	1.4651295441279342	0.0	0.0	0.0	0.0
7	1.469189549678827	0.0	0.0	0.0	0.0
8	1.4861617040309199	0.0	0.0	0.0	0.0
9	1.4909538417303345	0.0	0.0	0.0	6.655746804742352E-5
10	1.4964781115782706	0.0	0.0	0.0	6.655746804742352E-5
11	1.506262059381242	0.0	0.0	0.0	6.655746804742352E-5
12	1.5240994608179514	0.0	0.0	0.0	6.655746804742352E-5
13	1.5498572009523042	0.0	0.0	0.0	6.655746804742352E-5
14	1.596647100989643	0.0	0.0	0.0	6.655746804742352E-5
15	1.662805224228782	0.0	0.0	0.0	6.655746804742352E-5
16	1.7464679615643934	0.0	0.0	0.0	6.655746804742352E-5
17	1.8775196161497703	0.0	0.0	0.0	6.655746804742352E-5
18	2.067008727680785	0.0	0.0	0.0	6.655746804742352E-5
19	2.3215244854941326	0.0	0.0	0.0	6.655746804742352E-5
20	2.6568410095170525	0.0	0.0	0.0	6.655746804742352E-5
21	3.0810783108513298	0.0	0.0	0.0	6.655746804742352E-5
22	3.5791944017182478	0.0	0.0	0.0	1.3311493609484705E-4
23	4.165166350407764	0.0	0.0	0.0	1.3311493609484705E-4
24	4.883254873171417	0.0	0.0	0.0	1.3311493609484705E-4
25	5.673358576362381	6.655746804742352E-5	0.0	0.0	1.3311493609484705E-4
26	6.577075877510298	6.655746804742352E-5	0.0	0.0	1.3311493609484705E-4
27	7.592077265233507	6.655746804742352E-5	0.0	0.0	1.3311493609484705E-4
28	8.65500002995086	6.655746804742352E-5	0.0	0.0	1.3311493609484705E-4
29	9.829739340987885	6.655746804742352E-5	0.0	0.0	1.3311493609484705E-4
30	11.091003360486562	6.655746804742352E-5	0.0	0.0	1.3311493609484705E-4
31	12.36045394855507	6.655746804742352E-5	0.0	0.0	1.3311493609484705E-4
32	13.75283618010717	6.655746804742352E-5	0.0	0.0	1.3311493609484705E-4
33	15.185086335019678	6.655746804742352E-5	0.0	0.0	1.3311493609484705E-4
34	16.626521420522728	1.3311493609484705E-4	0.0	0.0	1.3311493609484705E-4
35	18.10076933777316	1.3311493609484705E-4	0.0	0.0	1.3311493609484705E-4
36	19.620342890763887	1.3311493609484705E-4	0.0	0.0	1.3311493609484705E-4
37	21.125939375464654	1.3311493609484705E-4	0.0	0.0	1.3311493609484705E-4
38	22.70548120716611	1.3311493609484705E-4	0.0	0.0	1.3311493609484705E-4
39	24.316770951126184	1.3311493609484705E-4	0.0	0.0	1.3311493609484705E-4
40	25.9554823719218	1.3311493609484705E-4	0.0	0.0	1.3311493609484705E-4
41	27.61735579159792	1.3311493609484705E-4	0.0	0.0	1.3311493609484705E-4
42	29.372276551604333	1.3311493609484705E-4	0.0	0.0	1.3311493609484705E-4
43	31.08353561257164	1.3311493609484705E-4	0.0	0.0	1.3311493609484705E-4
44	32.85023704442245	1.3311493609484705E-4	0.0	0.0	1.3311493609484705E-4
45	34.600232551793354	1.3311493609484705E-4	0.0	0.0	1.3311493609484705E-4
46	36.34856412246308	1.3311493609484705E-4	0.0	0.0	1.3311493609484705E-4
47	38.111338663699094	1.3311493609484705E-4	0.0	0.0	1.3311493609484705E-4
48	39.89707553141147	1.3311493609484705E-4	0.0	0.0	1.3311493609484705E-4
49	41.65372678558711	1.3311493609484705E-4	0.0	0.0	1.3311493609484705E-4
50	43.41510361999413	1.3311493609484705E-4	0.0	0.0	1.3311493609484705E-4
>>END_MODULE