##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-6A_S53_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1502461
Sequences flagged as poor quality	0
Sequence length	61
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.47894820564394	40.0	40.0	40.0	40.0	40.0
2	39.178895159341906	40.0	40.0	40.0	40.0	40.0
3	39.13148760600109	40.0	40.0	40.0	40.0	40.0
4	39.006069375511245	40.0	40.0	40.0	40.0	40.0
5	39.028500573392584	40.0	40.0	40.0	40.0	40.0
6	39.14621677367998	40.0	40.0	40.0	40.0	40.0
7	39.183299932577285	40.0	40.0	40.0	40.0	40.0
8	39.20678406960314	40.0	40.0	40.0	40.0	40.0
9	39.203562688149646	40.0	40.0	40.0	40.0	40.0
10	39.22003965493946	40.0	40.0	40.0	40.0	40.0
11	39.20950760119564	40.0	40.0	40.0	40.0	40.0
12	39.21729216265847	40.0	40.0	40.0	40.0	40.0
13	39.04007092363795	40.0	40.0	40.0	40.0	40.0
14	39.21039947126747	40.0	40.0	40.0	40.0	40.0
15	39.11435970717376	40.0	40.0	40.0	40.0	40.0
16	39.295899194721194	40.0	40.0	40.0	40.0	40.0
17	39.244098848489244	40.0	40.0	40.0	40.0	40.0
18	39.33590555761514	40.0	40.0	40.0	40.0	40.0
19	39.28085720694248	40.0	40.0	40.0	40.0	40.0
20	39.28474150077773	40.0	40.0	40.0	40.0	40.0
21	39.26784122849112	40.0	40.0	40.0	40.0	40.0
22	39.334949792373976	40.0	40.0	40.0	40.0	40.0
23	39.32794794673539	40.0	40.0	40.0	40.0	40.0
24	39.3265875120885	40.0	40.0	40.0	40.0	40.0
25	39.28654121471373	40.0	40.0	40.0	40.0	40.0
26	39.24955922316786	40.0	40.0	40.0	40.0	40.0
27	39.27593195430697	40.0	40.0	40.0	40.0	40.0
28	39.28129914853031	40.0	40.0	40.0	40.0	40.0
29	39.2830243181021	40.0	40.0	40.0	40.0	40.0
30	39.28724139927758	40.0	40.0	40.0	40.0	40.0
31	39.24020656775783	40.0	40.0	40.0	40.0	40.0
32	39.257391706007674	40.0	40.0	40.0	40.0	40.0
33	39.261683331547374	40.0	40.0	40.0	40.0	40.0
34	39.24999317785952	40.0	40.0	40.0	40.0	40.0
35	39.26058113987651	40.0	40.0	40.0	40.0	40.0
36	39.22166631945854	40.0	40.0	40.0	40.0	40.0
37	39.23229155365763	40.0	40.0	40.0	40.0	40.0
38	39.223876027397715	40.0	40.0	40.0	40.0	40.0
39	37.99920397268215	40.0	40.0	40.0	40.0	40.0
40	39.207505552556775	40.0	40.0	40.0	40.0	40.0
41	39.23805543039054	40.0	40.0	40.0	40.0	40.0
42	39.20433741707772	40.0	40.0	40.0	40.0	40.0
43	39.219887903912316	40.0	40.0	40.0	40.0	40.0
44	39.19278304062468	40.0	40.0	40.0	40.0	40.0
45	39.02399862625386	40.0	40.0	40.0	40.0	40.0
46	39.05936726477426	40.0	40.0	40.0	40.0	40.0
47	39.14363700621846	40.0	40.0	40.0	40.0	40.0
48	39.222129559436155	40.0	40.0	40.0	40.0	40.0
49	39.18748772846683	40.0	40.0	40.0	40.0	40.0
50	39.18617521519693	40.0	40.0	40.0	40.0	40.0
51	39.14145392126651	40.0	40.0	40.0	40.0	40.0
52	39.15203389638733	40.0	40.0	40.0	40.0	40.0
53	39.05816923034941	40.0	40.0	40.0	40.0	40.0
54	38.94716468513992	40.0	40.0	40.0	40.0	40.0
55	38.99465210744239	40.0	40.0	40.0	40.0	40.0
56	38.988965437372414	40.0	40.0	40.0	40.0	40.0
57	38.89471740031854	40.0	40.0	40.0	40.0	40.0
58	38.87711694346808	40.0	40.0	40.0	40.0	40.0
59	38.812194126835905	40.0	40.0	40.0	40.0	40.0
60	38.75016789121315	40.0	40.0	40.0	40.0	40.0
61	38.59675292736384	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.07693131951371157
1101	2	-0.024736835624551645
1101	3	-0.029322471994561283
1101	4	-0.07532155005973351
1101	5	0.003906793954463694
1101	6	0.056974211693763266
1101	7	-0.0020543929585130627
1101	8	0.02773646000205332
1101	9	0.03939786563447001
1101	10	-0.024923466264439753
1101	11	-0.010929204908698864
1101	12	-0.041542516910681115
1101	13	-0.06297792088100351
1101	14	-8.41518690798182E-4
1101	15	0.06364759975769374
1101	16	0.06071820680933371
1101	17	0.049536497638627
1101	18	-0.05455111366757137
1101	19	-0.029352226077939747
1101	20	-0.0029634844412598227
1101	21	0.11197493556390015
1101	22	0.008566759653881206
1101	23	0.05349409765049984
1101	24	0.05520501214300566
1101	25	0.005710551357076099
1101	26	0.0033998287100374114
1101	27	-0.013699825723570314
1101	28	0.06374526133655678
1101	29	0.04773622718854398
1101	30	0.09282975392939363
1101	31	0.07679039099114249
1101	32	0.033971263307748245
1101	33	-0.008239404211174417
1101	34	0.04283379823426969
1101	35	-0.02720549729101407
1101	36	0.029519537499297144
1101	37	-0.059701326191920145
1101	38	-0.03922935190792742
1101	39	1.2290958912034426
1101	40	-0.05695192993635345
1101	41	-0.032210996275878756
1101	42	0.02922913509372904
1101	43	-0.03784780126419918
1101	44	0.05295860528896412
1101	45	-0.024374272913938455
1101	46	0.044971136049596794
1101	47	-0.08670880180319074
1101	48	0.11251473338441542
1101	49	0.07630279138761864
1101	50	0.0669846861033605
1101	51	0.09377468016065649
1101	52	-0.001048556335682349
1101	53	-0.0488360090156732
1101	54	0.16172604727301376
1101	55	-0.05662345538361535
1101	56	0.03892485091362374
1101	57	-0.027157831500389307
1101	58	-0.020668753148655128
1101	59	0.03457534234090076
1101	60	0.01074415884285429
1101	61	0.06235181478044893
1102	1	0.059523989279675504
1102	2	0.010266291832536467
1102	3	0.14240305158293864
1102	4	0.005490070188599816
1102	5	0.0017683468124261026
1102	6	0.028905650420632867
1102	7	0.020028964253121728
1102	8	-0.06296062611892239
1102	9	-0.07566615248759945
1102	10	-0.027711490768112412
1102	11	-0.03134814034233102
1102	12	-0.114358827754522
1102	13	-0.0840973157791467
1102	14	-0.027987382861027754
1102	15	-0.013300302870220548
1102	16	0.06972084196895878
1102	17	-0.06566827681617582
1102	18	-0.058970687662842636
1102	19	-0.03419004746191234
1102	20	-0.043594854402975614
1102	21	-0.0670763868615154
1102	22	0.015623984927792378
1102	23	0.009035903964282
1102	24	-0.054977121743107205
1102	25	0.0023773364863686197
1102	26	-0.0712298783161458
1102	27	-0.0030292717341851016
1102	28	-0.07617632514642736
1102	29	-0.04702408335489139
1102	30	0.049057856648119014
1102	31	-0.06684926090708387
1102	32	-0.07288849656806917
1102	33	0.022411904628192758
1102	34	-0.002670013923690817
1102	35	-0.0388338937533419
1102	36	-0.06593179046894448
1102	37	-0.008992152511588358
1102	38	-0.0800563819147726
1102	39	1.241752595731377
1102	40	-0.06202178070360276
1102	41	-0.023985930571740255
1102	42	0.015964468119932462
1102	43	-0.09216505808239361
1102	44	0.050588828173992795
1102	45	0.03016609580558338
1102	46	8.031976582074662E-4
1102	47	0.02674237605410923
1102	48	-0.0022354243435458443
1102	49	-0.05441035844054909
1102	50	-0.005131514981137286
1102	51	-0.05991462418963778
1102	52	-0.010222721883401675
1102	53	-0.026862109523648314
1102	54	0.03426690409352773
1102	55	0.02918789974447833
1102	56	-0.053195360414960646
1102	57	-0.031928334638266165
1102	58	0.0013229813072683783
1102	59	0.05558982568669535
1102	60	-0.010234829427723469
1102	61	-0.07328973007188466
1103	1	0.015271641684883264
1103	2	0.03551740148557769
1103	3	0.004425277910392822
1103	4	0.01034274521155254
1103	5	0.031895665829800635
1103	6	-0.06539840194522384
1103	7	0.04696092134064145
1103	8	-0.045335704773172836
1103	9	-0.014294075612056645
1103	10	-0.05716887733081677
1103	11	-0.003582395160144358
1103	12	-0.04121904933830933
1103	13	-0.04056833951946004
1103	14	-0.015380230827766184
1103	15	-0.03359221363932363
1103	16	0.0488570290092909
1103	17	-0.027797982534281118
1103	18	-0.024370959033262807
1103	19	0.026463258776850296
1103	20	0.0019042824352411003
1103	21	0.047412698512580675
1103	22	-0.07681607682065561
1103	23	-0.06488033076759336
1103	24	0.02338126416205455
1103	25	0.007540558645601436
1103	26	-0.013175258825746994
1103	27	0.015588692647916957
1103	28	-0.07363656272649166
1103	29	-0.05456144279471431
1103	30	-0.036657045863080384
1103	31	-0.05087952925519801
1103	32	0.03569152266992859
1103	33	0.04103163850965075
1103	34	-0.06153647971679277
1103	35	0.048742168176303835
1103	36	0.006429939082188696
1103	37	0.017131674221865012
1103	38	0.028474091341365693
1103	39	1.2342267380373428
1103	40	0.08186134315639748
1103	41	-0.029854829072547773
1103	42	0.08754991830534209
1103	43	0.03318273701524532
1103	44	-0.02091540199116082
1103	45	0.019059125322954173
1103	46	-0.015327735384644825
1103	47	0.02504241802662932
1103	48	-0.017351195608149794
1103	49	0.10461985682258756
1103	50	0.05039962148335775
1103	51	0.0940146552914598
1103	52	-0.020374693565756274
1103	53	0.00999833147805873
1103	54	0.06686550590592333
1103	55	-0.00972958425393955
1103	56	-0.11996494116394274
1103	57	0.09482515074460451
1103	58	-0.009114764520425922
1103	59	0.04429617302243116
1103	60	0.1079808215817053
1103	61	0.20210240867951512
1104	1	0.04453409073680348
1104	2	0.09961167757985123
1104	3	0.060479149964265844
1104	4	0.03687614942916895
1104	5	-0.008919857827827116
1104	6	0.07063491366703545
1104	7	0.05842326406614262
1104	8	0.02252465319244834
1104	9	-0.11063538342704504
1104	10	-0.014569196310084465
1104	11	0.01545605956252416
1104	12	0.06706944968703255
1104	13	0.038174173328314964
1104	14	0.0188069036751628
1104	15	0.07718432366352346
1104	16	-0.02131132115906098
1104	17	0.0695619324045822
1104	18	-0.036447514057186936
1104	19	-0.08246071783133857
1104	20	0.014236020872239408
1104	21	0.010075820965177229
1104	22	0.007079723285670525
1104	23	-0.03065881738818632
1104	24	0.04837080873054589
1104	25	0.004724680566560835
1104	26	0.013449231693478225
1104	27	0.05656264267449984
1104	28	-0.01670337116171794
1104	29	0.05627180193327064
1104	30	-0.04707774407324905
1104	31	-0.02427169654210104
1104	32	-0.04375983299195241
1104	33	0.17632208685272843
1104	34	0.03496644425981543
1104	35	0.03329790094256424
1104	36	-0.05957218744625692
1104	37	-0.0429718168289952
1104	38	-0.08131290139299807
1104	39	1.0764330112962455
1104	40	-0.0590690130371172
1104	41	-0.10429005856040874
1104	42	0.07294208559224558
1104	43	0.010132586386149
1104	44	-0.03136977023500265
1104	45	0.020336105652567937
1104	46	-0.02498961736231564
1104	47	0.014593011682485724
1104	48	-0.023574835516001258
1104	49	-0.024516418208428092
1104	50	0.0904974417917046
1104	51	-0.05451236934082715
1104	52	-0.014841466558841887
1104	53	-0.0030252375455148695
1104	54	0.016766267911947352
1104	55	-0.0775300450017653
1104	56	-0.01938581658481553
1104	57	0.10113810484703123
1104	58	0.1285145106877934
1104	59	0.019393107277259958
1104	60	-0.0036527066834040056
1104	61	0.11907423217765256
1201	1	0.022822963010142416
1201	2	-0.03582501173453778
1201	3	-0.005546791975199028
1201	4	-0.024404563161454007
1201	5	-0.0722074405895512
1201	6	-0.0010187603574323134
1201	7	0.047285871480291064
1201	8	-0.041357275223397494
1201	9	-0.05936024065969292
1201	10	0.07762719955914577
1201	11	-0.026171369107977682
1201	12	0.11722351923397412
1201	13	-0.16089243698994693
1201	14	0.027564659708232853
1201	15	-0.07495498693538849
1201	16	0.08349411429492193
1201	17	-4.413401374918635E-4
1201	18	-0.012101881600507625
1201	19	0.025828436184028192
1201	20	-0.1078810321060999
1201	21	0.05294776921132893
1201	22	-0.0038317555349109966
1201	23	-0.07311378742600283
1201	24	-0.061396927472223695
1201	25	0.010877926559864193
1201	26	-0.03695173056392065
1201	27	0.05349711597943951
1201	28	0.015777757540206494
1201	29	-0.032127924993687884
1201	30	0.05777606432049254
1201	31	0.008554631167228877
1201	32	0.06393566888812785
1201	33	0.05996193384513049
1201	34	0.015174109810352832
1201	35	0.0469744394166014
1201	36	0.040047460809653046
1201	37	0.03325440035077065
1201	38	0.026442243108157015
1201	39	1.1921865086901349
1201	40	0.07249498033110768
1201	41	0.07438345660489887
1201	42	0.0078357496473771
1201	43	0.023738906076665955
1201	44	-0.02028577860057368
1201	45	-0.08438888161486346
1201	46	0.07349907502956654
1201	47	-0.1277636954033241
1201	48	0.018477546829608116
1201	49	0.05301569496518255
1201	50	0.05958349231021742
1201	51	0.0246151839463451
1201	52	-0.03969700874971238
1201	53	0.01118270974705382
1201	54	0.018172377379563898
1201	55	0.02645322385598803
1201	56	0.07811634524347255
1201	57	-3.5560472027640344E-4
1201	58	-0.005264755265848464
1201	59	-0.03142916768129567
1201	60	0.052516771985473554
1201	61	0.17104445421204417
1202	1	0.06415648004340824
1202	2	0.03508703263415924
1202	3	0.07865124687320701
1202	4	0.019405806906192424
1202	5	-0.027058537861243792
1202	6	0.14035588275642397
1202	7	0.05196712371526502
1202	8	0.07787636580732027
1202	9	0.01686336344012318
1202	10	0.07518853134238412
1202	11	-0.001867248708151692
1202	12	-0.028409247054824505
1202	13	-0.014771524248963885
1202	14	0.036624781703565645
1202	15	0.004963772285080381
1202	16	-0.13339152166031454
1202	17	-0.09635953793794272
1202	18	0.03923502562535219
1202	19	-0.06271802777285984
1202	20	-0.02782914203502429
1202	21	-0.03021824753942326
1202	22	-0.05448752817631686
1202	23	0.0658758526201666
1202	24	0.0015337159987112159
1202	25	0.041115658010170364
1202	26	0.056082481484622804
1202	27	0.07505554158844774
1202	28	-0.00926477467049125
1202	29	0.03895517703769258
1202	30	0.07925141215064002
1202	31	0.1281159392139557
1202	32	0.07936130318181256
1202	33	0.017779062625493225
1202	34	0.07985867483625952
1202	35	3.8896192835835564E-4
1202	36	-0.18338171125577674
1202	37	-0.16857248516650714
1202	38	-0.14554681952164827
1202	39	1.1305015158910692
1202	40	-0.18180919682993135
1202	41	-0.16264260659716712
1202	42	-0.15201363704768767
1202	43	-0.17676131503407078
1202	44	-0.09655272762848455
1202	45	-0.14994292525804553
1202	46	-0.041624537997236644
1202	47	-0.07658159149211485
1202	48	-0.008971660912827417
1202	49	-0.04050305145821653
1202	50	-0.03906646798272817
1202	51	0.021972175354243006
1202	52	0.012814339623282933
1202	53	-0.05735439376940832
1202	54	-0.04101150986583235
1202	55	-0.011204230465423848
1202	56	-0.04801739553218454
1202	57	0.01801696867326541
1202	58	-0.034031855226992036
1202	59	-0.02228441987395513
1202	60	0.07136149548869497
1202	61	-0.20581131502368066
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2403	2	-0.018396995447034215
2403	3	0.02305330434414543
2403	4	-4.211528029998135E-4
2403	5	-0.008685777396920003
2403	6	0.009856897507816598
2403	7	0.0164381451543818
2403	8	0.03609730120449939
2403	9	0.06430851578325303
2403	10	0.07734845558201187
2403	11	0.09058200104241365
2403	12	0.039857690888474906
2403	13	-0.001998191669173366
2403	14	0.020877839905587336
2403	15	0.16354539546706093
2403	16	-0.015101433908412787
2403	17	0.07891748085941686
2403	18	0.03645849771987031
2403	19	0.052217330281138175
2403	20	0.020183343645783225
2403	21	-0.02078153056629617
2403	22	0.061812183229982054
2403	23	0.027629035809418667
2403	24	0.06002991312639949
2403	25	-0.04716010511837965
2403	26	0.0199216834212379
2403	27	0.09447819478113217
2403	28	0.06649487597390191
2403	29	0.03478241497932544
2403	30	0.022995565827692133
2403	31	0.03478924288365448
2403	32	0.03708446335168247
2403	33	0.03931309429327001
2403	34	0.049309622239455564
2403	35	-0.06210320372717604
2403	36	0.036415396431046076
2403	37	-0.0297917374023271
2403	38	0.029303431286059833
2403	39	1.2389071063343664
2403	40	-0.024674527256316026
2403	41	-0.02155339904518172
2403	42	0.053485656140473736
2403	43	0.05482734148725399
2403	44	-0.04521666848670236
2403	45	0.0842360463107994
2403	46	0.02964716540117962
2403	47	-0.028389414026186444
2403	48	-0.013309623483316102
2403	49	0.1068289964992033
2403	50	-0.017136099998587895
2403	51	-0.08491762793396873
2403	52	0.11314611731845758
2403	53	0.012960885611470019
2403	54	-0.006420841231246754
2403	55	-0.057356036693917645
2403	56	0.04884662053574118
2403	57	-0.0294035485057762
2403	58	-0.01134069541071625
2403	59	0.01214756932134975
2403	60	0.02598859518357699
2403	61	0.04970243320928347
2404	1	-0.014200851474761578
2404	2	-0.03196374141455749
2404	3	-0.10160421918055107
2404	4	-0.03442523251732155
2404	5	-0.004162162127471447
2404	6	0.038975085109825613
2404	7	-0.040735625853109525
2404	8	-0.07917792297293147
2404	9	0.02794069148304601
2404	10	0.012250064993153842
2404	11	0.03234376227439384
2404	12	0.034446646439121764
2404	13	-0.014070732016264742
2404	14	0.051885681410212214
2404	15	0.026407583790636124
2404	16	0.00595124867334107
2404	17	-0.003620887128136019
2404	18	-0.024976498744948117
2404	19	0.009261807217853857
2404	20	0.0522291903020502
2404	21	-0.014933294001011177
2404	22	0.054414579549664666
2404	23	0.05481396211105505
2404	24	0.02602769697607954
2404	25	-0.01811385108702268
2404	26	-0.012992584062068602
2404	27	0.04323950242162766
2404	28	0.05597244816743796
2404	29	0.08562714990784315
2404	30	-0.0060204984667535655
2404	31	0.03097974534151149
2404	32	0.05195644954937961
2404	33	-0.06364136281139565
2404	34	-0.008881654715594323
2404	35	0.07309687409525623
2404	36	-0.0034529464990242786
2404	37	0.003146383700261879
2404	38	0.051481706527752635
2404	39	1.2697897163377547
2404	40	0.02824286356026562
2404	41	-0.009399234594603456
2404	42	0.015359745556509097
2404	43	0.05275244932239076
2404	44	0.09054695109130506
2404	45	0.021899084866824126
2404	46	0.024895077184531544
2404	47	0.1315960650830732
2404	48	0.10973256276052723
2404	49	0.019431199008231204
2404	50	0.08408412444160263
2404	51	-0.055032397386874266
2404	52	-0.002356088954968527
2404	53	-0.049141266767684044
2404	54	0.04181229509852358
2404	55	0.05976821569078794
2404	56	-0.004410311642828901
2404	57	0.0521393035894846
2404	58	0.02380612175241481
2404	59	-0.04255138868546737
2404	60	0.07302085086754317
2404	61	0.055817904853107336
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	13.0
17	43.0
18	144.0
19	228.0
20	321.0
21	394.0
22	543.0
23	820.0
24	1196.0
25	1560.0
26	1996.0
27	2564.0
28	3615.0
29	4352.0
30	5436.0
31	6928.0
32	9322.0
33	11333.0
34	14469.0
35	18621.0
36	27373.0
37	40201.0
38	106133.0
39	669004.0
40	575852.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	27.593727890441084	15.633018094978837	26.93141452590117	29.841839488678907
2	31.389349021028185	16.408363870720326	26.239161003046636	25.963126105204847
3	27.21629860362339	21.398173661861215	19.469603184111392	31.915924550404007
4	26.629327902240323	21.502402726195704	20.796360635224897	31.071908736339072
5	26.849881627543077	21.89381288432778	19.711460064520807	31.54484542360833
6	28.480805824577143	24.655016003743192	22.708742523100433	24.155435648579232
7	26.966357196626067	24.36316150635524	22.9847563430931	25.685724953925593
8	24.577210323595754	26.981532299340884	23.562342050808642	24.878915326254724
9	24.40036713099375	26.86033115002652	23.99895904119974	24.74034267777999
10	25.888192771725855	24.409618619052342	23.900121201149314	25.80206740807249
11	24.7685630442321	25.825695309229324	23.656254638223555	25.749487008315025
12	26.377257046938325	24.681039973749737	23.60620342225189	25.335499557060047
13	24.47091804712402	23.21504518253718	24.036031550902155	28.278005219436643
14	24.423462572406205	24.360366092697248	24.07909423272884	27.137077102167712
15	24.905205526133457	23.44406943008837	23.776723655389393	27.874001388388788
16	24.970099057479693	24.847966103612674	23.42104054614396	26.76089429276367
17	25.264016836377117	23.28672757562426	23.27175214531359	28.177503442685037
18	26.659061366651116	23.840286037374682	23.375714910403662	26.124937685570544
19	24.969100695458984	23.244796370754383	24.83891428795822	26.947188645828412
20	25.18501312180483	23.55561974653585	23.211584194198718	28.047782937460607
21	25.21383250546936	24.074701439837707	24.717979368516055	25.99348668617688
22	26.49306704134084	23.50703279486123	23.452721900934534	26.547178262863397
23	25.07486051218634	24.972095781521116	23.304964321869253	26.64807938442329
24	25.026939135192194	25.595805814593525	23.374783105850998	26.002471944363283
25	25.006239762629445	23.621378524966705	23.425366781567043	27.947014930836804
26	25.43080985130396	23.943849457656473	24.57394900766143	26.05139168337814
27	25.479796147786864	24.610489057619468	22.9381661154599	26.971548679133768
28	25.46135972913773	24.28568861354804	22.901226720693582	27.351724936620652
29	25.684327247096594	25.985366675075095	22.620420763001505	25.709885314826806
30	27.253952016058985	25.223350223400143	22.5752282421973	24.94746951834357
31	25.761068007755277	24.89715207249972	23.909638919080095	25.43214100066491
32	25.966530911617674	25.223283665932094	22.096680046936328	26.713505375513908
33	26.178117102540433	27.08469637481439	21.873512856573317	24.863673666071865
34	26.01897819643904	25.676606580803096	21.760165488488553	26.54424973426931
35	26.35076717465545	25.735975842301396	22.93257528814392	24.980681694899236
36	26.374461633280333	26.362947191308127	22.674066082247727	24.588525093163817
37	27.712067068629402	26.176186935967056	21.072493728622575	25.03925226678097
38	27.913802754281143	26.343778640510468	20.74503098582925	24.99738761937914
39	26.870310913644563	28.138914419408508	20.477766466498235	24.513008200448695
40	26.370135397857247	26.84695309894899	20.472278481770907	26.310633021422852
41	26.61579901242029	26.793973354383244	21.656335838334574	24.933891794861896
42	27.889642393379926	27.42227585275092	20.081519586864484	24.606562167004668
43	26.359685875373806	28.572788245418685	20.046377243735446	25.021148635472066
44	26.39356362660994	27.233851660708662	21.308839297658974	25.06374541502242
45	26.350301272379117	27.615891527300874	19.753724056730924	26.280083143589085
46	26.045002166445585	27.523975663927384	21.098517698629117	25.332504470997918
47	27.717591338477337	27.473791333019626	19.5605742844573	25.248043044045737
48	27.584409844914443	27.939959839223782	19.419805239537	25.05582507632478
49	27.454090322477587	27.780088800973868	19.46579644995777	25.300024426590774
50	27.44057915646396	27.877795164067486	19.315110342298404	25.36651533717015
51	27.36084330974315	28.071144608745254	19.210681674931994	25.357330406579603
52	26.9435279850858	28.10701908402281	19.262463385072888	25.686989545818495
53	27.197378168218677	27.92804605244329	19.113041869306425	25.76153391003161
54	26.890881027860292	28.204592332180333	19.188584595540252	25.715942044419123
55	26.78884842934359	28.123192548758336	19.20974987037933	25.878209151518742
56	26.73919655818021	27.993871388342196	19.089147738277397	26.177784315200192
57	26.678629262257058	28.15334308178382	19.1485169997757	26.01951065618342
58	26.411600700450794	28.030544553236325	19.29347916518299	26.264375581129894
59	26.51523067820063	27.905283398371072	19.285159481677063	26.29432644175123
60	26.461851588826597	27.90628176039178	19.415479004113916	26.216387646667698
61	26.753972316086738	27.60151511420263	19.453217088496807	26.19129548121382
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	2.5
10	5.0
11	3.0
12	2.5
13	4.0
14	10.0
15	16.0
16	17.5
17	32.0
18	45.0
19	65.0
20	120.0
21	155.0
22	234.5
23	314.0
24	412.5
25	656.5
26	802.0
27	1069.0
28	1336.0
29	1805.0
30	2982.5
31	3691.0
32	4618.5
33	5546.0
34	7096.0
35	10734.0
36	12822.0
37	16024.0
38	19226.0
39	23494.0
40	33074.5
41	38387.0
42	45196.5
43	60136.0
44	68266.0
45	76764.5
46	85263.0
47	94179.5
48	110627.5
49	118159.0
50	123488.0
51	128817.0
52	130693.5
53	130286.5
54	128003.0
55	122159.0
56	116315.0
57	108580.0
58	92017.0
59	83189.0
60	75133.5
61	68926.0
62	70774.0
63	54732.5
64	38691.0
65	33545.5
66	24487.5
67	20575.0
68	17483.0
69	14391.0
70	12001.5
71	7891.5
72	6171.0
73	4949.0
74	3727.0
75	2974.5
76	1742.0
77	1262.0
78	968.5
79	675.0
80	506.5
81	259.5
82	181.0
83	138.5
84	65.0
85	34.0
86	30.0
87	26.0
88	20.0
89	10.5
90	7.0
91	5.5
92	4.0
93	2.5
94	1.5
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.031747912258621024
3	6.655746804742352E-5
4	6.655746804742352E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.1073685107300624
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1502461.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.4809225115567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.57067090634771	76.59673327448446
2	9.642750450107904	16.678279089481205
3	1.3739641621105565	3.564650647114169
4	0.2690039812373208	0.9305484982673997
5	0.07368892671879634	0.31863431807640036
6	0.031597234321328015	0.16395347837533608
7	0.015832082533630554	0.09584211719512321
8	0.005991809763568456	0.0414541788693722
9	0.005512135114385741	0.042902507673038166
>10	0.010569449480481361	0.1428247772732641
>50	8.475253024093177E-5	0.004031212350233413
>100	2.505823005588292E-4	0.03871811815108035
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	8.352743351960973E-5	1.381427782688919
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTGATCTGGGGGGGGGGGGGGG	19124	1.2728450189389275	TruSeq Adapter, Index 3 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4469593553509874	0.0	0.0	0.0	0.0
2	1.4492888667326473	0.0	0.0	0.0	1.3311493609484705E-4
3	1.450353786221406	6.655746804742352E-5	6.655746804742352E-5	6.655746804742352E-5	1.3311493609484705E-4
4	1.451751493050402	6.655746804742352E-5	6.655746804742352E-5	6.655746804742352E-5	1.3311493609484705E-4
5	1.4542141193681566	6.655746804742352E-5	6.655746804742352E-5	6.655746804742352E-5	1.996724041422706E-4
6	1.4606036363007093	6.655746804742352E-5	6.655746804742352E-5	6.655746804742352E-5	1.996724041422706E-4
7	1.465262659064029	6.655746804742352E-5	6.655746804742352E-5	6.655746804742352E-5	1.996724041422706E-4
8	1.510987639612609	6.655746804742352E-5	6.655746804742352E-5	6.655746804742352E-5	1.996724041422706E-4
9	1.5170443692049245	6.655746804742352E-5	6.655746804742352E-5	6.655746804742352E-5	1.996724041422706E-4
10	1.5225686390528606	6.655746804742352E-5	6.655746804742352E-5	6.655746804742352E-5	1.996724041422706E-4
11	1.5312211098990256	1.996724041422706E-4	6.655746804742352E-5	6.655746804742352E-5	1.996724041422706E-4
12	1.5487257239954981	1.996724041422706E-4	6.655746804742352E-5	6.655746804742352E-5	1.996724041422706E-4
13	1.5742837917257086	1.996724041422706E-4	6.655746804742352E-5	6.655746804742352E-5	2.662298721896941E-4
14	1.6204081170825733	1.996724041422706E-4	6.655746804742352E-5	6.655746804742352E-5	3.993448082845412E-4
15	1.685767550705143	1.996724041422706E-4	6.655746804742352E-5	6.655746804742352E-5	3.993448082845412E-4
16	1.7675001214673791	1.996724041422706E-4	6.655746804742352E-5	6.655746804742352E-5	3.993448082845412E-4
17	1.8977530864361871	2.662298721896941E-4	6.655746804742352E-5	6.655746804742352E-5	3.993448082845412E-4
18	2.08005399141808	2.662298721896941E-4	6.655746804742352E-5	6.655746804742352E-5	3.993448082845412E-4
19	2.3364333583367554	2.662298721896941E-4	6.655746804742352E-5	6.655746804742352E-5	3.993448082845412E-4
20	2.6700859456584896	2.662298721896941E-4	6.655746804742352E-5	6.655746804742352E-5	3.993448082845412E-4
21	3.093457999908151	2.662298721896941E-4	6.655746804742352E-5	6.655746804742352E-5	3.993448082845412E-4
22	3.595567538857914	2.662298721896941E-4	6.655746804742352E-5	6.655746804742352E-5	3.993448082845412E-4
23	4.182804079440332	3.3278734023711764E-4	6.655746804742352E-5	6.655746804742352E-5	5.324597443793882E-4
24	4.900360142459605	3.3278734023711764E-4	6.655746804742352E-5	6.655746804742352E-5	5.324597443793882E-4
25	5.693525489180751	3.3278734023711764E-4	6.655746804742352E-5	6.655746804742352E-5	5.324597443793882E-4
26	6.5979083650091415	3.3278734023711764E-4	6.655746804742352E-5	6.655746804742352E-5	5.324597443793882E-4
27	7.613974672221109	3.3278734023711764E-4	6.655746804742352E-5	6.655746804742352E-5	5.324597443793882E-4
28	8.645815099360316	3.3278734023711764E-4	6.655746804742352E-5	6.655746804742352E-5	5.324597443793882E-4
29	9.780486814632793	3.3278734023711764E-4	6.655746804742352E-5	6.655746804742352E-5	5.324597443793882E-4
30	11.009603577064563	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	5.324597443793882E-4
31	12.234327546605202	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	5.324597443793882E-4
32	13.577923154078542	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	5.990172124268118E-4
33	14.970371943098689	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	6.655746804742353E-4
34	16.367679427286298	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	6.655746804742353E-4
35	17.79420563994673	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	6.655746804742353E-4
36	19.27377815464095	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	6.655746804742353E-4
37	20.742501802043446	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	6.655746804742353E-4
38	22.293290807548416	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	6.655746804742353E-4
39	23.8630486914469	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	6.655746804742353E-4
40	25.508482416515303	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	7.321321485216588E-4
41	27.178076502484924	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	7.321321485216588E-4
42	28.938588089807322	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	7.321321485216588E-4
43	30.666153730446247	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	7.321321485216588E-4
44	32.45209027056276	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	7.321321485216588E-4
45	34.22491498947394	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	7.321321485216588E-4
46	36.00006921976677	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	7.986896165690824E-4
47	37.79106412745489	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	7.986896165690824E-4
48	39.5938397069874	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	7.986896165690824E-4
49	41.364601144389106	3.3278734023711764E-4	1.3311493609484705E-4	6.655746804742352E-5	7.986896165690824E-4
50	43.13995504708608	3.3278734023711764E-4	1.996724041422706E-4	6.655746804742352E-5	7.986896165690824E-4
>>END_MODULE