##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-5C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 609943 Sequences flagged as poor quality 0 Sequence length 31-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.263003264239444 40.0 40.0 40.0 40.0 40.0 2 39.160472372008535 40.0 40.0 40.0 40.0 40.0 3 39.131095856498064 40.0 40.0 40.0 40.0 40.0 4 39.072831395720584 40.0 40.0 40.0 40.0 40.0 5 39.191235902371204 40.0 40.0 40.0 40.0 40.0 6 39.258343812454605 40.0 40.0 40.0 40.0 40.0 7 39.277939086111324 40.0 40.0 40.0 40.0 40.0 8 39.31377850061399 40.0 40.0 40.0 40.0 40.0 9 39.334462400584975 40.0 40.0 40.0 40.0 40.0 10-11 39.32191696601158 40.0 40.0 40.0 40.0 40.0 12-13 39.3175296708053 40.0 40.0 40.0 40.0 40.0 14-15 39.328484792841294 40.0 40.0 40.0 40.0 40.0 16-17 39.46502542040814 40.0 40.0 40.0 40.0 40.0 18-19 39.47318683877018 40.0 40.0 40.0 40.0 40.0 20-21 39.48588310711001 40.0 40.0 40.0 40.0 40.0 22-23 39.48906045318989 40.0 40.0 40.0 40.0 40.0 24-25 39.48099740467552 40.0 40.0 40.0 40.0 40.0 26-27 39.47770201477843 40.0 40.0 40.0 40.0 40.0 28-29 39.474167258251995 40.0 40.0 40.0 40.0 40.0 30-31 39.465022141413215 40.0 40.0 40.0 40.0 40.0 32-33 39.460562399503516 40.0 40.0 40.0 40.0 40.0 34-35 39.45668270684722 40.0 40.0 40.0 40.0 40.0 36-37 39.44915789664351 40.0 40.0 40.0 40.0 40.0 38-39 39.43917385701347 40.0 40.0 40.0 40.0 40.0 40-41 39.43063052416349 40.0 40.0 40.0 40.0 40.0 42-43 39.42384367671274 40.0 40.0 40.0 40.0 40.0 44-45 39.40162403176559 40.0 40.0 40.0 40.0 40.0 46-47 39.39899046512956 40.0 40.0 40.0 40.0 40.0 48-49 39.38566225168391 40.0 40.0 40.0 40.0 40.0 50-51 39.387570472215515 40.0 40.0 40.0 40.0 40.0 52-53 39.38476434527591 40.0 40.0 40.0 40.0 40.0 54-55 39.35146399389134 40.0 40.0 40.0 40.0 40.0 56-57 39.35322333322118 40.0 40.0 40.0 40.0 40.0 58-59 39.340649343009424 40.0 40.0 40.0 40.0 40.0 60-61 39.30128111444442 40.0 40.0 40.0 40.0 40.0 62-63 39.36055103298396 40.0 40.0 40.0 40.0 40.0 64-65 39.359966519648864 40.0 40.0 40.0 40.0 40.0 66-67 39.34426189247138 40.0 40.0 40.0 40.0 40.0 68-69 39.339455352659996 40.0 40.0 40.0 40.0 40.0 70-71 39.33677930436233 40.0 40.0 40.0 40.0 40.0 72-73 39.345693714731155 40.0 40.0 40.0 40.0 40.0 74-75 39.32728222774178 40.0 40.0 40.0 40.0 40.0 76-77 39.324143440557165 40.0 40.0 40.0 40.0 40.0 78-79 39.30132474226 40.0 40.0 40.0 40.0 40.0 80-81 39.291325842004284 40.0 40.0 40.0 40.0 40.0 82-83 39.27175041907466 40.0 40.0 40.0 40.0 40.0 84-85 39.281066347211336 40.0 40.0 40.0 40.0 40.0 86-87 39.30604367806124 40.0 40.0 40.0 40.0 40.0 88-89 39.373703454098745 40.0 40.0 40.0 40.0 40.0 90-91 39.47955529541943 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20734477534880114 1101 2 -0.00254889248191148 1101 3 0.10939614038474588 1101 4 0.38253892168854264 1101 5 0.1515218553430131 1101 6 0.2635237543341731 1101 7 -0.014568001502041739 1101 8 -0.041296267632965566 1101 9 0.13223578464820207 1101 10-11 -0.01629699919200789 1101 12-13 0.040733565757662404 1101 14-15 -0.02356325585571284 1101 16-17 -0.0710663706259993 1101 18-19 -0.08842499371640855 1101 20-21 0.006020993525922336 1101 22-23 0.00920786757616554 1101 24-25 0.08577342382412212 1101 26-27 0.0887854470080498 1101 28-29 -0.014050997733043857 1101 30-31 0.11169340017462304 1101 32-33 0.06775120403516866 1101 34-35 0.08170922480137932 1101 36-37 0.16555497534351815 1101 38-39 0.13086083254466985 1101 40-41 0.10780438338569809 1101 42-43 0.07264449388920724 1101 44-45 -0.0054792567552297555 1101 46-47 0.07395347020595011 1101 48-49 0.10548612434030957 1101 50-51 0.11661755332547585 1101 52-53 0.1472002973466573 1101 54-55 0.12945997771220874 1101 56-57 0.1139071918863479 1101 58-59 0.15673547012192302 1101 60-61 0.12442675032851014 1101 62-63 0.06953039704540487 1101 64-65 0.10270836756497204 1101 66-67 0.07335162362623038 1101 68-69 -0.07731669829562549 1101 70-71 0.1225293577312172 1101 72-73 0.1537451428513208 1101 74-75 0.1727568729030864 1101 76-77 0.35523965908169686 1101 78-79 0.15294370480865638 1101 80-81 0.16674170312285952 1101 82-83 0.15462124093199492 1101 84-85 0.011265870890575513 1101 86-87 0.16609184505612262 1101 88-89 0.5698802174865634 1101 90-91 -0.13515251882938628 1102 1 0.3748815600605866 1102 2 0.17162557292079583 1102 3 -9.754867051015026E-6 1102 4 -0.09876835659298422 1102 5 -0.13131769273437754 1102 6 -0.08648998437180211 1102 7 0.12867013068047584 1102 8 0.061765578094835405 1102 9 0.043004236269105434 1102 10-11 -0.01582287914265379 1102 12-13 -0.12122961451031955 1102 14-15 -0.058578099045895726 1102 16-17 0.11680369892956577 1102 18-19 0.003610867000055862 1102 20-21 -0.02666365737625398 1102 22-23 -0.0024484815917062974 1102 24-25 0.04642415359185037 1102 26-27 -0.08855161463294081 1102 28-29 -0.024670209292949608 1102 30-31 0.004038516468725106 1102 32-33 -0.05381454483393355 1102 34-35 0.018942049991970578 1102 36-37 -0.19892673519986914 1102 38-39 0.004755855291392663 1102 40-41 -0.04663409667769258 1102 42-43 -0.06166125691342472 1102 44-45 0.005796898509096593 1102 46-47 -0.05028821846264009 1102 48-49 0.03718254247159791 1102 50-51 0.011447862323677782 1102 52-53 0.02073723368405922 1102 54-55 0.06549716589894672 1102 56-57 0.05280050267406011 1102 58-59 -0.026965917296664088 1102 60-61 -0.016898842360433264 1102 62-63 -0.01221063801391864 1102 64-65 0.033639565316875064 1102 66-67 0.2242746629177148 1102 68-69 0.046199767173597195 1102 70-71 0.11495368780085613 1102 72-73 -0.07612460451774439 1102 74-75 -0.14493396980272877 1102 76-77 0.20478415449888843 1102 78-79 0.233919614043117 1102 80-81 0.33738755250934815 1102 82-83 0.267641699162148 1102 84-85 0.176793239047349 1102 86-87 0.44867410796625506 1102 88-89 0.3345860998395054 1102 90-91 0.3648474811706137 1103 1 0.4109788063699682 1103 2 0.30381030142917353 1103 3 0.08488336866128066 1103 4 -0.019705499691838213 1103 5 -0.03332938146722597 1103 6 -0.05733336381148035 1103 7 0.02033080493283279 1103 8 0.04910043420639454 1103 9 0.006385297419924996 1103 10-11 -0.04204586040402347 1103 12-13 -0.0016034001651874519 1103 14-15 0.012110358859153791 1103 16-17 -0.0547485478733023 1103 18-19 -0.07797409133504374 1103 20-21 -0.05105895227353585 1103 22-23 -1.510956943135966E-4 1103 24-25 -0.0416158686858239 1103 26-27 -0.05335763665683402 1103 28-29 0.07986657708100608 1103 30-31 -0.02029068562368508 1103 32-33 0.07384399221008664 1103 34-35 -0.07940983793120182 1103 36-37 0.06694023058634002 1103 38-39 -0.042688328056442515 1103 40-41 0.07171948293658659 1103 42-43 0.010607636598621184 1103 44-45 -0.10624137722644633 1103 46-47 -0.007169735689913637 1103 48-49 -0.05032475884278398 1103 50-51 -0.12241573116804716 1103 52-53 -0.0040181975237914 1103 54-55 0.06531370461410546 1103 56-57 0.022979519650569102 1103 58-59 -0.05835999802594927 1103 60-61 -0.004931161796477568 1103 62-63 0.009058706206793943 1103 64-65 0.06804953322053109 1103 66-67 -0.040731575079291815 1103 68-69 -0.1121570371682239 1103 70-71 0.00624205254700172 1103 72-73 -0.029283789040874808 1103 74-75 0.13473057381009568 1103 76-77 -0.07743002110530739 1103 78-79 -0.11889742604609665 1103 80-81 -0.05431360045700018 1103 82-83 -0.12649809972073456 1103 84-85 0.07049715619366026 1103 86-87 0.18981534377962106 1103 88-89 -0.314222346615999 1103 90-91 -0.40203705041979987 1104 1 0.2968687554881555 1104 2 0.20384873857152286 1104 3 0.3145599451012089 1104 4 0.11808734651822306 1104 5 0.12237896508067081 1104 6 0.07696394317546407 1104 7 0.09756705374352492 1104 8 0.01816801463137807 1104 9 0.056555248192807994 1104 10-11 0.0058152763701571075 1104 12-13 -0.011210962112762957 1104 14-15 0.12357031528556917 1104 16-17 0.0466151950425413 1104 18-19 0.09329698876589276 1104 20-21 0.059075526015512025 1104 22-23 0.05153996620499157 1104 24-25 0.11927115024661816 1104 26-27 0.07532664100067876 1104 28-29 -0.03965311376716585 1104 30-31 0.05693522135498341 1104 32-33 0.021530432877774786 1104 34-35 0.12730153722754523 1104 36-37 0.01069128222670912 1104 38-39 -0.08308534228620346 1104 40-41 -0.048604561827161774 1104 42-43 -0.008414511519916346 1104 44-45 -0.04230175092565247 1104 46-47 -0.04680696063653755 1104 48-49 -0.05257521442668178 1104 50-51 -0.04466268316932087 1104 52-53 -0.007846333418932261 1104 54-55 0.01555080611994697 1104 56-57 0.023610094219563393 1104 58-59 -0.06701759942956187 1104 60-61 0.05427967976865489 1104 62-63 0.11622182887268906 1104 64-65 -0.22560695025781285 1104 66-67 -0.011632830559804574 1104 68-69 -0.07520399741941475 1104 70-71 -0.13160937386238913 1104 72-73 -0.1315739551603201 1104 74-75 -0.1508830821338094 1104 76-77 -0.09971337278485493 1104 78-79 0.15384658340519053 1104 80-81 0.4080652615449267 1104 82-83 0.47666392044359185 1104 84-85 0.2206590715729746 1104 86-87 -0.37291838783954745 1104 88-89 -0.455152720589858 1104 90-91 -0.41293029660716485 1201 1 0.2968554783943773 1201 2 0.18818010827800435 1201 3 0.22905546117769404 1201 4 0.2609936857496322 1201 5 0.2457829131904674 1201 6 0.1593317136941721 1201 7 -0.005011772777137935 1201 8 0.07476992503920599 1201 9 0.1458171496551941 1201 10-11 0.07922017877106668 1201 12-13 0.0844496687317502 1201 14-15 0.021483571053018125 1201 16-17 0.12151211085663505 1201 18-19 0.14231767862708722 1201 20-21 0.06841928076103443 1201 22-23 0.12749656006616306 1201 24-25 0.0423071568951201 1201 26-27 0.07055435560622669 1201 28-29 0.09148391164934111 1201 30-31 0.07155828051356394 1201 32-33 0.12718810370187583 1201 34-35 0.05472616210385439 1201 36-37 0.020862476830551202 1201 38-39 -0.05260643162606016 1201 40-41 0.04348327750953729 1201 42-43 0.09648109138151995 1201 44-45 0.03795851247645743 1201 46-47 0.053835826942957965 1201 48-49 0.041834495151327644 1201 50-51 0.03832019794779029 1201 52-53 0.17053340645674098 1201 54-55 0.07051965337735311 1201 56-57 0.1362397367077648 1201 58-59 0.06069639112581626 1201 60-61 0.04371402147823744 1201 62-63 0.040994262085028765 1201 64-65 0.06120091724291399 1201 66-67 -0.13391531187888006 1201 68-69 -0.07727198492613496 1201 70-71 -0.14581094757533464 1201 72-73 -0.030932112458756933 1201 74-75 -0.29472206617901264 1201 76-77 -0.12314982651460582 1201 78-79 -0.3748770712177887 1201 80-81 -0.13096163574518727 1201 82-83 -0.14543117413432327 1201 84-85 -0.16960646596979245 1201 86-87 0.3744273004503995 1201 88-89 0.10313195147269028 1201 90-91 0.3648474811706137 1202 1 0.059018358498299506 1202 2 0.1981885228618836 1202 3 0.2518679730727129 1202 4 0.21830202315257452 1202 5 0.01707468544437063 1202 6 -0.15496716681792577 1202 7 -0.026693701506665946 1202 8 0.1376015175498253 1202 9 -0.009950646703771326 1202 10-11 0.015503394713263674 1202 12-13 0.07028417435672907 1202 14-15 0.05214780486020487 1202 16-17 0.0782268111103761 1202 18-19 0.05733281111022137 1202 20-21 -0.06414402501231109 1202 22-23 0.02958449449234024 1202 24-25 -0.044192173744576735 1202 26-27 0.06336672982276781 1202 28-29 0.13344749812478085 1202 30-31 0.04408413774423536 1202 32-33 -0.012870081138046885 1202 34-35 0.07086890312033489 1202 36-37 0.12279375181176277 1202 38-39 0.07132135629572645 1202 40-41 -0.023504188110806012 1202 42-43 0.10098677334263328 1202 44-45 0.07484543315123204 1202 46-47 0.039521253284100055 1202 48-49 0.013879694970796663 1202 50-51 0.115319635170124 1202 52-53 -0.04214605313541142 1202 54-55 -0.039400057945243816 1202 56-57 0.024162046223281664 1202 58-59 0.04943143145995066 1202 60-61 0.03971318587267092 1202 62-63 -0.14069884892549567 1202 64-65 -0.17912018809199992 1202 66-67 -0.12026498112686568 1202 68-69 -0.0534120987977289 1202 70-71 0.08580416150813619 1202 72-73 -0.1265925963924417 1202 74-75 -0.022666653954033222 1202 76-77 -0.10975485259331919 1202 78-79 0.1627801140244074 1202 80-81 0.12719761173384114 1202 82-83 0.2054090792083869 1202 84-85 -0.5358569891984075 1202 86-87 -0.28470180573752657 1202 88-89 -0.332080566827166 1202 90-91 0.3648474811706137 1203 1 0.23925564404687805 1203 2 0.13579088636755188 1203 3 0.09345012179632306 1203 4 0.1307477872263476 1203 5 0.10649568808828747 1203 6 0.07105704760772369 1203 7 0.03994411658187147 1203 8 0.1433296784369844 1203 9 0.05153758031935496 1203 10-11 -0.01398928186340953 1203 12-13 -0.0034682944542367977 1203 14-15 -0.046871543265815774 1203 16-17 -0.03366012164247678 1203 18-19 -0.09997984394589565 1203 20-21 0.01238861563550131 1203 22-23 -0.008979102038317421 1203 24-25 0.005805368931945054 1203 26-27 -0.09145104265943615 1203 28-29 -0.02397863333283823 1203 30-31 0.03837116031924381 1203 32-33 0.0010396964589887148 1203 34-35 -8.571233125707067E-4 1203 36-37 0.11855952315730889 1203 38-39 -0.08415135027460963 1203 40-41 0.11057670283744869 1203 42-43 0.042063034153059675 1203 44-45 0.019749181800172266 1203 46-47 0.16605352273757035 1203 48-49 0.06948624038487594 1203 50-51 -0.02419817830000426 1203 52-53 0.030859470571002134 1203 54-55 -0.05302901111559066 1203 56-57 -0.01022208987622264 1203 58-59 -0.08924017034853904 1203 60-61 -0.19262686420989894 1203 62-63 -0.09518800885535228 1203 64-65 0.030710996954624648 1203 66-67 -0.04195459305370264 1203 68-69 0.03707983706865292 1203 70-71 -0.016014341748494587 1203 72-73 0.002739010718961765 1203 74-75 -0.051978868378846244 1203 76-77 -0.19832270087847803 1203 78-79 0.18801686049278032 1203 80-81 0.4385423056872284 1203 82-83 0.10038093721328067 1203 84-85 -0.42738636957592036 1203 86-87 -0.17266902144864815 1203 88-89 -0.6088482911100002 1203 90-91 -0.7069473906242649 1204 1 0.0414404621000628 1204 2 0.03288914414144273 1204 3 -0.095362869822182 1204 4 -0.02043957538614194 1204 5 -0.07598365335693558 1204 6 -0.2511939722823513 1204 7 -0.1401815567030127 1204 8 -0.03385509041745394 1204 9 -0.2317385840674433 1204 10-11 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48-49 0.16110467903936865 2401 50-51 0.0760838794865819 2401 52-53 0.09670522990036545 2401 54-55 0.1532497914772648 2401 56-57 0.17920125877448356 2401 58-59 0.0529258225429885 2401 60-61 0.15695570476722054 2401 62-63 0.11018303845282418 2401 64-65 0.10871544010753098 2401 66-67 0.19521155369086785 2401 68-69 0.0672609325513136 2401 70-71 0.010218152324654284 2401 72-73 0.11194869237699834 2401 74-75 0.2027224647093604 2401 76-77 0.17606111269838465 2401 78-79 0.18676658144751457 2401 80-81 0.05504557208045924 2401 82-83 0.2325854146848414 2401 84-85 0.3249230190803942 2401 86-87 0.013004520194598967 2401 88-89 -0.23077605814921043 2401 90-91 0.17437129069442392 2402 1 0.36979039651554046 2402 2 0.27920923759938887 2402 3 0.16512552991547835 2402 4 0.24313744543694327 2402 5 0.23952196231776668 2402 6 0.1001064458189802 2402 7 0.11352089451850134 2402 8 0.1911777018435714 2402 9 0.12015209473236155 2402 10-11 0.11119462111893341 2402 12-13 0.12201432099942622 2402 14-15 0.11501139469341837 2402 16-17 0.05590374775939466 2402 18-19 0.08055054472318801 2402 20-21 0.11012525825277919 2402 22-23 0.04597152468642207 2402 24-25 0.02886285471842598 2402 26-27 0.14598796804641978 2402 28-29 0.0018815546845871722 2402 30-31 0.0790059269183061 2402 32-33 0.003924698727793441 2402 34-35 0.0033041207631754332 2402 36-37 0.10045134800217426 2402 38-39 0.0985292752468041 2402 40-41 0.06949720691855532 2402 42-43 0.05271506743971344 2402 44-45 0.09568251442519227 2402 46-47 0.09812380361365314 2402 48-49 0.06598828338593421 2402 50-51 0.055736783128502054 2402 52-53 0.055870512157483176 2402 54-55 0.11171796759303732 2402 56-57 0.1306299288993671 2402 58-59 0.16266768962031364 2402 60-61 0.1759162863907875 2402 62-63 0.035212081785168436 2402 64-65 0.08088296831431308 2402 66-67 -0.03195367442652497 2402 68-69 -0.03602748678493839 2402 70-71 0.021038601215494168 2402 72-73 0.1515774566080097 2402 74-75 0.05597495034995603 2402 76-77 0.040358945415221115 2402 78-79 0.03738239265355503 2402 80-81 0.26303964742594843 2402 82-83 0.10192272873654673 2402 84-85 0.04583745611746082 2402 86-87 -0.06138853364376473 2402 88-89 0.21580105241083203 2402 90-91 0.3648474811706137 2403 1 -0.30152998292142286 2403 2 -0.2389083674597714 2403 3 0.031825346316949776 2403 4 0.09660286771356397 2403 5 0.09204389214232833 2403 6 0.19485093786304475 2403 7 0.12309606791711047 2403 8 0.08882152069302407 2403 9 0.09494554638474284 2403 10-11 0.11554233633721367 2403 12-13 0.016298541725660698 2403 14-15 0.1389620780879568 2403 16-17 0.02114752580917667 2403 18-19 0.035186407636288664 2403 20-21 0.1026283182282981 2403 22-23 0.03566960710175948 2403 24-25 0.07598647772944389 2403 26-27 0.028159785671874715 2403 28-29 0.08839598756912181 2403 30-31 0.05551423985180293 2403 32-33 0.027528405617964324 2403 34-35 0.04021863665735026 2403 36-37 0.07440292708134422 2403 38-39 0.06663511330398109 2403 40-41 0.05852222774249327 2403 42-43 0.12366082035396175 2403 44-45 0.10514046062517934 2403 46-47 0.1317882989918715 2403 48-49 0.0612464997761748 2403 50-51 0.017278483553674562 2403 52-53 0.08935764028254312 2403 54-55 0.12068012335609524 2403 56-57 -0.0429709600234105 2403 58-59 0.10450868679329517 2403 60-61 0.09667576270513933 2403 62-63 0.16970122047789005 2403 64-65 0.15154092899709326 2403 66-67 0.15106492017021367 2403 68-69 0.31429214079074796 2403 70-71 0.08431011342219108 2403 72-73 -0.053719975546329124 2403 74-75 0.11660633238609819 2403 76-77 -0.02775460510785166 2403 78-79 -0.037730184671723066 2403 80-81 -0.07555119487444983 2403 82-83 0.2580716844907798 2403 84-85 0.1213232480363331 2403 86-87 0.10213273376876941 2403 88-89 -0.04597344105002321 2403 90-91 -1.0947484784253447 2404 1 -0.4572426940073413 2404 2 -0.25435935188287573 2404 3 -0.5496403582558216 2404 4 -0.40496865986912667 2404 5 -0.1780729000419683 2404 6 -0.10311208895047486 2404 7 -0.09655819767795037 2404 8 -0.019957428072530092 2404 9 0.05137688392711226 2404 10-11 0.0703376951206991 2404 12-13 0.01685207337568073 2404 14-15 0.17119964163057233 2404 16-17 0.05498248056776589 2404 18-19 0.015218352134091617 2404 20-21 0.03447406851733348 2404 22-23 -0.002266937347179976 2404 24-25 0.03721996845670361 2404 26-27 0.013073719650257942 2404 28-29 0.011300405393704693 2404 30-31 0.019205025229716455 2404 32-33 0.07075412233482581 2404 34-35 0.03979552941596154 2404 36-37 0.05889690622404942 2404 38-39 0.0774348678060619 2404 40-41 0.018746454057065876 2404 42-43 -2.6792090176286365E-4 2404 44-45 0.04030811625865027 2404 46-47 0.0747929230606843 2404 48-49 0.12391105114221546 2404 50-51 0.06819614354625259 2404 52-53 0.08333483333778702 2404 54-55 0.12949473335748962 2404 56-57 0.01709242511905984 2404 58-59 0.07206830010578358 2404 60-61 -0.04307059000493041 2404 62-63 -0.030344312247208904 2404 64-65 0.13437581516371466 2404 66-67 -0.04758253355160491 2404 68-69 0.11989952633995671 2404 70-71 0.0043372926353555386 2404 72-73 -0.11297606382268555 2404 74-75 0.07451381017315128 2404 76-77 0.08011034367822134 2404 78-79 -0.07616518161297137 2404 80-81 -0.13924776389231397 2404 82-83 -0.031085160022463754 2404 84-85 0.38166636308873336 2404 86-87 -0.3264697682868274 2404 88-89 -0.05605122811701335 2404 90-91 -0.5142733979502623 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 14.0 31 315.0 32 2378.0 33 4321.0 34 5956.0 35 7289.0 36 10636.0 37 16742.0 38 45218.0 39 273338.0 40 243736.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.009410761714268 13.95055251336197 24.55421844771617 32.48581827720759 2 28.692598161304762 17.29904949917116 25.562356428792782 28.4459959107313 3 26.85658823220797 18.77410088927363 19.633863224994098 34.735447653524304 4 26.545103394907393 20.31140614778758 18.740931529667527 34.4025589276375 5 26.92890975058325 20.816535315595065 18.93816307425448 33.316391859567204 6 27.132863234761277 23.809110031593118 22.329627522571784 26.72839921107382 7 25.493365773523102 23.474980448992774 22.486527429612273 28.54512634787185 8 24.545900190673557 24.88576801438823 23.366773616551054 27.20155817838716 9 24.308337008540143 24.737885343384548 23.936662934077447 27.017114713997863 10-11 24.411953248090395 23.86468899552909 23.608189617718377 28.11516813866214 12-13 24.67017409823541 23.46104472057225 23.560972090834717 28.307809090357622 14-15 24.853715839021024 22.926732497954728 23.47899721777281 28.740554445251444 16-17 25.230144456121312 22.885745061423773 23.098387882146366 28.785722600308556 18-19 25.08062228765639 23.077894163880888 23.148556504460252 28.69292704400247 20-21 25.20825716501378 23.203971518650103 23.00665799919009 28.581113317146027 22-23 25.181123482030287 22.819509363989752 23.128472660560085 28.87089449341988 24-25 25.019960881590574 23.078467987992322 23.205365091492155 28.69620603892495 26-27 25.249818425656166 23.118553700919595 23.09953553036923 28.532092343055005 28-29 25.321136565220026 22.806475359172907 23.044448415671628 28.827939659935435 30-31 25.364419298196715 23.073959369973913 23.113881133155065 28.447740198674303 32-33 25.42619409032002 23.01519019242468 23.062505684952257 28.496110032303047 34-35 25.468988271114434 22.718704756614695 23.118753787693713 28.69355318457715 36-37 25.5362059963904 22.9370457631356 23.12574150979566 28.40100673067834 38-39 25.74443212572286 23.00937126687134 23.01503808191107 28.231158525494727 40-41 25.73929709789798 22.818199777375078 23.02933254312883 28.413170581598113 42-43 25.709866389748754 22.947194026183013 23.129615023325314 28.21332456074292 44-45 25.880426985677524 22.973785205400958 22.9690100081905 28.176777800731017 46-47 25.889763676320882 22.71759123736789 23.026703832996187 28.365941253315043 48-49 25.927630430232735 22.966174112031414 23.00040124373996 28.10579421399589 50-51 25.981094559155693 22.974829612625257 22.867054836101232 28.177020992117818 52-53 26.046641688174947 22.877112647396295 22.859389646091355 28.2168560183374 54-55 26.12716306970991 22.970275269683704 22.88588684625387 28.01667481435251 56-57 26.136908193893184 23.052313066020012 22.750030045745596 28.060748694341207 58-59 26.318545622744054 22.773790152722828 22.729187488126605 28.17847673640651 60-61 26.233075042622477 22.904101680543405 22.83157581388636 28.03124746294776 62-63 26.532501174311417 22.87538821645384 22.52517584941859 28.066934759816153 64-65 26.430808094097834 22.775175644028103 22.479146383180275 28.314869878693788 66-67 26.448019862468048 22.913194563235862 22.515114341151573 28.123671233144513 68-69 26.721494629293613 22.806218105854935 22.363714722570695 28.108572542280758 70-71 26.893700162714946 22.576931812171573 22.24901297675012 28.280355048363358 72-73 26.73765274448941 22.82567929104411 22.1262961626798 28.310371801786676 74-75 27.098687384584668 22.620572626395383 22.019997005440118 28.260742983579828 76-77 27.05399695376603 22.50970372917997 21.965803567041714 28.470495750012283 78-79 27.255279447916358 22.570269229630068 21.743328495690548 28.431122826763023 80-81 27.74267997867352 22.651348607570903 21.189900598163877 28.4160708155917 82-83 27.78448115834416 22.19231483200297 20.81214033785038 29.21106367180249 84-85 28.35121637658228 21.533821202531644 20.307555379746837 29.80740704113924 86-87 29.515566647197293 20.46938883874051 19.630435936919238 30.384608577142956 88-89 28.304834905660375 19.73172169811321 19.902712264150942 32.06073113207547 90-91 24.958354457883683 22.148185702904325 22.314767871369597 30.578691967842396 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 1.0 16 2.0 17 6.0 18 14.5 19 23.0 20 32.0 21 45.0 22 62.5 23 87.0 24 128.0 25 195.0 26 288.5 27 415.5 28 569.0 29 747.0 30 988.0 31 1324.5 32 1790.0 33 2356.0 34 3077.0 35 3990.0 36 5181.0 37 6542.0 38 8179.5 39 10286.5 40 12589.0 41 15341.5 42 18294.0 43 21333.0 44 24831.5 45 28365.0 46 31933.0 47 35608.5 48 38989.5 49 42247.0 50 45008.5 51 47053.0 52 48595.0 53 49444.5 54 49705.5 55 49163.5 56 47826.0 57 46126.5 58 44153.0 59 41696.0 60 39115.0 61 36585.0 62 34147.0 63 31522.0 64 28620.5 65 25932.0 66 23290.0 67 20938.5 68 18557.5 69 15971.0 70 13502.0 71 11188.5 72 9113.5 73 7180.5 74 5531.5 75 4288.0 76 3271.0 77 2359.5 78 1686.5 79 1211.0 80 837.5 81 573.0 82 395.0 83 253.0 84 164.0 85 112.5 86 64.5 87 35.5 88 25.0 89 16.0 90 7.0 91 4.0 92 2.5 93 1.5 94 2.5 95 2.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.918492383714543E-4 2 0.00885328629068618 3 0.0011476482228667268 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 7009.0 32-33 15479.0 34-35 16907.0 36-37 18559.0 38-39 20253.0 40-41 21471.0 42-43 22687.0 44-45 23689.0 46-47 25098.0 48-49 25475.0 50-51 25947.0 52-53 25943.0 54-55 26201.0 56-57 26075.0 58-59 25772.0 60-61 24945.0 62-63 24406.0 64-65 23674.0 66-67 22712.0 68-69 21703.0 70-71 20871.0 72-73 20068.0 74-75 18763.0 76-77 17612.0 78-79 16373.0 80-81 14874.0 82-83 13771.0 84-85 12404.0 86-87 11551.0 88-89 10330.0 90-91 9321.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.73558902610877 #Duplication Level Percentage of deduplicated Percentage of total 1 99.74651584423557 99.48277511026926 2 0.2445521959934583 0.48781114630071915 3 0.007512294488014757 0.022477293470992258 4 6.426607102580487E-4 0.0025638457792609565 5 2.778309598282292E-4 0.00138548172140788 6 4.99173612868566E-4 0.0029871224583442337 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.836984767429087E-4 0.0 0.0 0.0 0.0 2 9.836984767429087E-4 0.0 0.0 0.0 0.0 3 9.836984767429087E-4 0.0 0.0 0.0 0.0 4 9.836984767429087E-4 0.0 0.0 0.0 0.0 5 9.836984767429087E-4 0.0 0.0 0.0 1.6394974612381812E-4 6 9.836984767429087E-4 0.0 0.0 0.0 3.2789949224763625E-4 7 0.001311597968990545 0.0 0.0 0.0 4.918492383714543E-4 8 0.004262693399219271 0.0 0.0 0.0 4.918492383714543E-4 9 0.004426643145343089 0.0 0.0 0.0 4.918492383714543E-4 10-11 0.004426643145343089 0.0 0.0 0.0 4.918492383714543E-4 12-13 0.004426643145343089 0.0 0.0 0.0 4.918492383714543E-4 14-15 0.004590592891466907 0.0 0.0 0.0 4.918492383714543E-4 16-17 0.004590592891466907 0.0 0.0 0.0 5.738241114333634E-4 18-19 0.004590592891466907 0.0 0.0 0.0 8.197487306190906E-4 20-21 0.004590592891466907 0.0 0.0 0.0 8.197487306190906E-4 22-23 0.0047545426375907255 0.0 0.0 0.0 8.197487306190906E-4 24-25 0.004918492383714544 0.0 0.0 0.0 9.836984767429087E-4 26-27 0.005820215987395544 0.0 0.0 0.0 9.836984767429087E-4 28-29 0.0064760149718908165 0.0 0.0 0.0 0.0010656733498048178 30-31 0.0068858893372003615 0.0 8.197487306190906E-5 0.0 0.0011476482228667268 32-33 0.007377738575571816 0.0 1.6394974612381812E-4 0.0 0.0011476482228667268 34-35 0.007541688321695634 0.0 1.6394974612381812E-4 0.0 0.0011476482228667268 36-37 0.007705638067819452 0.0 1.6394974612381812E-4 0.0 0.0011476482228667268 38-39 0.007951562687005179 8.197487306190906E-5 1.6394974612381812E-4 0.0 0.0011476482228667268 40-41 0.008279462179252815 1.6394974612381812E-4 1.6394974612381812E-4 0.0 0.0011476482228667268 42-43 0.00868933654456236 1.6394974612381812E-4 1.6394974612381812E-4 0.0 0.0011476482228667268 44-45 0.009017236036809997 1.6394974612381812E-4 1.6394974612381812E-4 0.0 0.0011476482228667268 46-47 0.009017236036809997 1.6394974612381812E-4 1.6394974612381812E-4 0.0 0.0011476482228667268 48-49 0.009181185782933815 1.6394974612381812E-4 1.6394974612381812E-4 0.0 0.001311597968990545 50-51 0.009345135529057634 1.6394974612381812E-4 1.6394974612381812E-4 0.0 0.001393572842052454 52-53 0.009345135529057634 1.6394974612381812E-4 1.6394974612381812E-4 0.0 0.0014755477151143631 54-55 0.009345135529057634 1.6394974612381812E-4 1.6394974612381812E-4 0.0 0.0014755477151143631 56-57 0.009427110402119541 1.6394974612381812E-4 1.6394974612381812E-4 0.0 0.0014755477151143631 58-59 0.00967303502130527 1.6394974612381812E-4 1.6394974612381812E-4 0.0 0.0014755477151143631 60-61 0.009836984767429087 1.6394974612381812E-4 1.6394974612381812E-4 0.0 0.0015575225881762722 62-63 0.010000934513552906 1.6394974612381812E-4 1.6394974612381812E-4 0.0 0.0016394974612381813 64-65 0.010000934513552906 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