##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-5B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 813949 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.246490873506815 40.0 40.0 40.0 40.0 40.0 2 39.143631849169914 40.0 40.0 40.0 40.0 40.0 3 39.099498862950874 40.0 40.0 40.0 40.0 40.0 4 39.05092333794869 40.0 40.0 40.0 40.0 40.0 5 39.190730623171724 40.0 40.0 40.0 40.0 40.0 6 39.262061873655476 40.0 40.0 40.0 40.0 40.0 7 39.305499484611445 40.0 40.0 40.0 40.0 40.0 8 39.317018633845606 40.0 40.0 40.0 40.0 40.0 9 39.339540929468555 40.0 40.0 40.0 40.0 40.0 10-11 39.33728525988729 40.0 40.0 40.0 40.0 40.0 12-13 39.33803960690412 40.0 40.0 40.0 40.0 40.0 14-15 39.349236868649015 40.0 40.0 40.0 40.0 40.0 16-17 39.47763803383259 40.0 40.0 40.0 40.0 40.0 18-19 39.49294611824574 40.0 40.0 40.0 40.0 40.0 20-21 39.504624982646334 40.0 40.0 40.0 40.0 40.0 22-23 39.51195468020724 40.0 40.0 40.0 40.0 40.0 24-25 39.512529654806386 40.0 40.0 40.0 40.0 40.0 26-27 39.497486943285146 40.0 40.0 40.0 40.0 40.0 28-29 39.49569076195192 40.0 40.0 40.0 40.0 40.0 30-31 39.49046621274347 40.0 40.0 40.0 40.0 40.0 32-33 39.49368785123549 40.0 40.0 40.0 40.0 40.0 34-35 39.487334871340515 40.0 40.0 40.0 40.0 40.0 36-37 39.48802858436568 40.0 40.0 40.0 40.0 40.0 38-39 39.467861675595216 40.0 40.0 40.0 40.0 40.0 40-41 39.46141986559441 40.0 40.0 40.0 40.0 40.0 42-43 39.45775514295112 40.0 40.0 40.0 40.0 40.0 44-45 39.43831908504887 40.0 40.0 40.0 40.0 40.0 46-47 39.4402205061523 40.0 40.0 40.0 40.0 40.0 48-49 39.44138069786655 40.0 40.0 40.0 40.0 40.0 50-51 39.42933373168167 40.0 40.0 40.0 40.0 40.0 52-53 39.421313332649575 40.0 40.0 40.0 40.0 40.0 54-55 39.40662391900878 40.0 40.0 40.0 40.0 40.0 56-57 39.40388875895921 40.0 40.0 40.0 40.0 40.0 58-59 39.38815977392234 40.0 40.0 40.0 40.0 40.0 60-61 39.359765291381336 40.0 40.0 40.0 40.0 40.0 62-63 39.351457275020664 40.0 40.0 40.0 40.0 40.0 64-65 39.35685002298334 40.0 40.0 40.0 40.0 40.0 66-67 39.352363325390414 40.0 40.0 40.0 40.0 40.0 68-69 39.34563834283548 40.0 40.0 40.0 40.0 40.0 70-71 39.34559487889 40.0 40.0 40.0 40.0 40.0 72-73 39.34733383561546 40.0 40.0 40.0 40.0 40.0 74-75 39.335467147641566 40.0 40.0 40.0 40.0 40.0 76-77 39.33412508494703 40.0 40.0 40.0 40.0 40.0 78-79 39.33396268509766 40.0 40.0 40.0 40.0 40.0 80-81 39.32397740928499 40.0 40.0 40.0 40.0 40.0 82-83 39.32550159252501 40.0 40.0 40.0 40.0 40.0 84-85 39.30164762409201 40.0 40.0 40.0 40.0 40.0 86-87 39.307470443154145 40.0 40.0 40.0 40.0 40.0 88-89 39.326085594646884 40.0 40.0 40.0 40.0 40.0 90-91 39.42005523226532 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2294919697610851 1101 2 0.18706900902322587 1101 3 0.19488880917779738 1101 4 0.29424455497954227 1101 5 0.140989227313149 1101 6 0.10928032451673175 1101 7 0.20079150244010435 1101 8 0.11313128108970005 1101 9 0.09604741141355788 1101 10-11 0.007573624097766185 1101 12-13 0.09759837813702177 1101 14-15 0.012963892504501473 1101 16-17 0.05716093878885431 1101 18-19 0.028265446797121285 1101 20-21 0.00966025987732877 1101 22-23 0.02007740523753654 1101 24-25 0.019436812029866246 1101 26-27 0.09637333953575222 1101 28-29 0.0882951447053486 1101 30-31 0.1307265410267462 1101 32-33 0.12512756948866155 1101 34-35 0.11140426611959953 1101 36-37 0.09184544362008751 1101 38-39 0.0824377322897476 1101 40-41 0.16478898392653463 1101 42-43 0.08520673644928678 1101 44-45 0.0062512092777922135 1101 46-47 0.13800161035744196 1101 48-49 0.05622557743763679 1101 50-51 0.12072375988029194 1101 52-53 0.16338971520169565 1101 54-55 0.14243548991233723 1101 56-57 0.02444726758491811 1101 58-59 -0.042836833832090804 1101 60-61 0.12155926342727241 1101 62-63 -0.022499966499843538 1101 64-65 -0.008835280201182627 1101 66-67 -0.03331517721814237 1101 68-69 -0.07779232672297809 1101 70-71 0.14470843597256788 1101 72-73 -0.016798420379622314 1101 74-75 -0.1334308365194019 1101 76-77 0.06105202870105586 1101 78-79 0.05157508667797117 1101 80-81 -0.01915729434622193 1101 82-83 -0.05484567613549984 1101 84-85 -0.052163711490997855 1101 86-87 -0.3883710516913652 1101 88-89 -0.10476867758995922 1101 90-91 0.577190446607311 1102 1 0.38935848964549535 1102 2 0.14329234740220187 1102 3 0.20584014168547782 1102 4 -0.05088869578877109 1102 5 0.04215201801082458 1102 6 0.1376276480340053 1102 7 -0.04451834573200131 1102 8 0.13055020682705276 1102 9 0.08245858521292604 1102 10-11 0.06284058432894568 1102 12-13 -0.14721837338813515 1102 14-15 -0.10367639922523608 1102 16-17 0.10422873384412412 1102 18-19 -0.034925461876795794 1102 20-21 -0.018784536232935523 1102 22-23 -0.012518576598161246 1102 24-25 0.11877858534301566 1102 26-27 0.02592358952428242 1102 28-29 -0.011224372747982159 1102 30-31 -0.012047872990955 1102 32-33 0.07233717909395665 1102 34-35 -0.023013818210102954 1102 36-37 -0.06942864490457623 1102 38-39 -0.06898534313195626 1102 40-41 -0.0636075778746985 1102 42-43 0.0848237622806991 1102 44-45 -0.0038878617400754933 1102 46-47 -0.05206122187293971 1102 48-49 0.020898793866955145 1102 50-51 -0.03508625833685386 1102 52-53 -0.07555781136834128 1102 54-55 0.14597663070528455 1102 56-57 0.013969626417541292 1102 58-59 0.00828342122908765 1102 60-61 -0.02386861245663141 1102 62-63 -0.11094537835452911 1102 64-65 -0.02942948260396605 1102 66-67 -0.1149855720790427 1102 68-69 -0.0776213074963863 1102 70-71 -0.09073455648186268 1102 72-73 0.0899753383647024 1102 74-75 0.039638670972685475 1102 76-77 -0.17193565259604782 1102 78-79 -0.05234876639459429 1102 80-81 0.004561076898305316 1102 82-83 -0.11680962875169598 1102 84-85 -0.016611112391679228 1102 86-87 -0.2954364424409661 1102 88-89 -0.37377127306641 1102 90-91 0.577190446607311 1103 1 0.3072497378198591 1103 2 0.13276263163992752 1103 3 0.09957680048785988 1103 4 0.251532380494794 1103 5 -0.007803043218252981 1103 6 0.07945440907209189 1103 7 0.04264040047629436 1103 8 0.02002891708432486 1103 9 0.03579528895407691 1103 10-11 0.03176823290775843 1103 12-13 0.07931235349648347 1103 14-15 0.04539392995125979 1103 16-17 0.04460341084671171 1103 18-19 -0.0024094524396502948 1103 20-21 0.056119968878846294 1103 22-23 0.030468166464416413 1103 24-25 0.07831683947176771 1103 26-27 0.04372776882997442 1103 28-29 0.08395875274353415 1103 30-31 0.08798863976041815 1103 32-33 0.0670521943597393 1103 34-35 0.09523104360993528 1103 36-37 0.040562579255521314 1103 38-39 0.1449640187702883 1103 40-41 0.058908488404064485 1103 42-43 0.06856434317254667 1103 44-45 0.0012655932361766986 1103 46-47 0.07802801849896213 1103 48-49 0.0211060081817962 1103 50-51 0.06566413012792793 1103 52-53 -0.05706706252001936 1103 54-55 0.01939698114572508 1103 56-57 -0.019026169840891782 1103 58-59 0.02327572317634008 1103 60-61 0.05446794584978676 1103 62-63 0.023564742748668266 1103 64-65 -0.0642969942542777 1103 66-67 -0.020518287127799795 1103 68-69 0.06651789337018954 1103 70-71 0.001586897395959852 1103 72-73 -0.12025921795487449 1103 74-75 0.05039282339937046 1103 76-77 0.16159564591449538 1103 78-79 0.05321250264688615 1103 80-81 -0.07220375993344419 1103 82-83 -8.24709038440119E-4 1103 84-85 0.21075045316461427 1103 86-87 0.13592857104199396 1103 88-89 0.5153736322075275 1103 90-91 0.577190446607311 1104 1 0.31939373555599815 1104 2 0.2562400839876702 1104 3 0.19770709377915097 1104 4 0.1253868061411012 1104 5 -0.060777392863514024 1104 6 0.1264376120312818 1104 7 -0.011013107497753083 1104 8 -0.02253975673775699 1104 9 0.08868878641734312 1104 10-11 -0.023926792661747243 1104 12-13 -0.0536248041450591 1104 14-15 -0.05144677103716333 1104 16-17 0.04720685110704892 1104 18-19 0.0013218757595723218 1104 20-21 -0.008436455837660617 1104 22-23 0.01502539970275052 1104 24-25 -0.009282457114245801 1104 26-27 -0.0022528670724355493 1104 28-29 0.07335094646536078 1104 30-31 -0.04338402815945841 1104 32-33 -0.015848251900820287 1104 34-35 -0.01966911335146193 1104 36-37 0.002346738435839768 1104 38-39 -0.015026835319034149 1104 40-41 -0.036052109978392366 1104 42-43 -0.10738456033906374 1104 44-45 0.04265985218263779 1104 46-47 -0.06820543699137716 1104 48-49 -0.04133367793389908 1104 50-51 -0.0073245189452748605 1104 52-53 -0.006639639763633909 1104 54-55 -0.02350078733330463 1104 56-57 -0.015799752090877917 1104 58-59 0.030896635290488916 1104 60-61 0.021034560013653447 1104 62-63 -0.04051643263728266 1104 64-65 -0.02193078323367814 1104 66-67 -0.11512812408082596 1104 68-69 -0.14629586126535088 1104 70-71 0.122151741797353 1104 72-73 -0.10940597843277544 1104 74-75 0.10875031557848303 1104 76-77 -0.2686055940379646 1104 78-79 -0.10794436988044964 1104 80-81 -0.3935913946436713 1104 82-83 -0.3476502062606954 1104 84-85 -0.45964886295843854 1104 86-87 -0.1789121227476258 1104 88-89 0.19674372410393914 1104 90-91 0.15613781502836588 1201 1 0.32339456018703316 1201 2 0.28542603806575784 1201 3 0.21764873155183295 1201 4 0.11621729627952249 1201 5 0.023936064353691222 1201 6 0.14200439295378686 1201 7 0.20351065157885273 1201 8 0.03509552796566595 1201 9 0.0312629597566314 1201 10-11 0.07233193906540691 1201 12-13 0.0563635502517883 1201 14-15 0.007713685044528518 1201 16-17 -0.024955404949679405 1201 18-19 0.036544709854496205 1201 20-21 0.1387769051212473 1201 22-23 0.015099766108669144 1201 24-25 0.04321186474663108 1201 26-27 0.03790323197393519 1201 28-29 0.0118411280095998 1201 30-31 0.02066260881785098 1201 32-33 0.07985882903870589 1201 34-35 0.08093883214672104 1201 36-37 -0.03182224377226106 1201 38-39 0.03170796113306551 1201 40-41 -0.0037734241892906084 1201 42-43 -0.007586038653535354 1201 44-45 6.278179509067172E-4 1201 46-47 0.0353616746023917 1201 48-49 0.04609429212185745 1201 50-51 0.10701379856806881 1201 52-53 -0.0027855791682682707 1201 54-55 0.09566628527105081 1201 56-57 -0.007216270392319757 1201 58-59 0.0035220657780286047 1201 60-61 0.011039078017027748 1201 62-63 -0.06925709577934214 1201 64-65 -0.15783111684885398 1201 66-67 0.03557807643788635 1201 68-69 0.06016480042663375 1201 70-71 -0.06331241299237433 1201 72-73 -0.11001182794645104 1201 74-75 -0.2624502403654887 1201 76-77 0.031060389905064767 1201 78-79 -0.08349250556155141 1201 80-81 -0.022510197758222716 1201 82-83 -0.04842745229809964 1201 84-85 0.20147791267320514 1201 86-87 0.16889262990067522 1201 88-89 -0.007825390991499148 1201 90-91 -0.5899183067083413 1202 1 0.04363765985452517 1202 2 0.13494269231142653 1202 3 -0.04347470322195335 1202 4 0.15512147750883543 1202 5 0.06725793007421288 1202 6 0.08482765951304572 1202 7 -0.02441926728353394 1202 8 0.078755530948456 1202 9 0.11164852383594592 1202 10-11 0.13016917897181912 1202 12-13 -0.014170043050121706 1202 14-15 0.010146784127286423 1202 16-17 -0.0014452291861957178 1202 18-19 -0.0027729449183055976 1202 20-21 0.009603214915863134 1202 22-23 -0.03648062044091205 1202 24-25 -0.07822233656724364 1202 26-27 -0.008250314317244545 1202 28-29 -0.014477514237889011 1202 30-31 -0.03531400014282582 1202 32-33 0.06944994343458433 1202 34-35 0.0011166767272570155 1202 36-37 0.007005735517786604 1202 38-39 -0.013136892369416842 1202 40-41 0.033606974066458406 1202 42-43 0.06969071714605235 1202 44-45 0.04275467076001149 1202 46-47 -0.08275104115003984 1202 48-49 -0.07611876670630835 1202 50-51 0.027908145126865236 1202 52-53 0.020962325342289034 1202 54-55 -0.01165233027133894 1202 56-57 -0.08688561716871845 1202 58-59 0.06417134267869784 1202 60-61 0.029254104251556612 1202 62-63 -0.013995705628126132 1202 64-65 -0.012486189172932427 1202 66-67 -0.07736953051748685 1202 68-69 -0.09809990493504017 1202 70-71 -0.0014802355261025468 1202 72-73 -0.17549334140044692 1202 74-75 0.16883517150008487 1202 76-77 0.05971672387848059 1202 78-79 -0.09650964427640218 1202 80-81 -0.13810896421315277 1202 82-83 0.1918219940570367 1202 84-85 0.04884650548683567 1202 86-87 0.07151239819208399 1202 88-89 -0.14445851009305954 1202 90-91 0.301328377641795 1203 1 0.1752755671860342 1203 2 0.2387782013497599 1203 3 0.2177202158896563 1203 4 0.2522321822469351 1203 5 0.061523045936134224 1203 6 -0.015245647632951886 1203 7 -0.010541016094769873 1203 8 0.06500612517424287 1203 9 -0.019411684447298683 1203 10-11 0.046549347051929146 1203 12-13 -0.005339921518576318 1203 14-15 -0.08721234804410471 1203 16-17 0.009379469890284042 1203 18-19 0.012336495787323543 1203 20-21 -0.006878892624577304 1203 22-23 0.010507112717711209 1203 24-25 -0.03403299107247193 1203 26-27 -0.034242981918396254 1203 28-29 0.08506735828304812 1203 30-31 0.05468915362238391 1203 32-33 0.022470849025928885 1203 34-35 0.05312930475638922 1203 36-37 0.0250687535600278 1203 38-39 0.04336823376165455 1203 40-41 -0.013517097821896584 1203 42-43 0.08662938710849488 1203 44-45 0.03783345209215838 1203 46-47 0.017322785646115335 1203 48-49 -0.07340683533219305 1203 50-51 0.014096007896483798 1203 52-53 -0.009929018499036601 1203 54-55 -0.024802645196110973 1203 56-57 0.10745107491408135 1203 58-59 0.14254424763889517 1203 60-61 -0.02811181488034009 1203 62-63 -0.03250765216486684 1203 64-65 0.06811122676035097 1203 66-67 -0.022652932132984915 1203 68-69 -0.09195207512543391 1203 70-71 -0.011218911484291993 1203 72-73 0.20743619035830108 1203 74-75 -0.12186295056464047 1203 76-77 -0.16528700263798157 1203 78-79 -0.20507223203448177 1203 80-81 -0.09895022847026524 1203 82-83 -0.16897730719517767 1203 84-85 -0.610855639570481 1203 86-87 0.014156710401614703 1203 88-89 -0.009621024978073933 1203 90-91 0.2914761608930263 1204 1 0.297845608102115 1204 2 0.24130395985338282 1204 3 -0.009583535899842843 1204 4 -0.029012740535002024 1204 5 0.01722611994348 1204 6 -0.09666497474508162 1204 7 -0.01876212821062495 1204 8 -0.045078242819698744 1204 9 -0.14368625959375692 1204 10-11 -0.12573681312188256 1204 12-13 -0.08775221641912623 1204 14-15 -0.17670200775408773 1204 16-17 -0.15326002636098934 1204 18-19 -0.1419649737485571 1204 20-21 -0.12415310533578605 1204 22-23 -0.20040561537158652 1204 24-25 -0.006968873896632033 1204 26-27 -0.045930813487196076 1204 28-29 -0.02565278324006215 1204 30-31 -0.08784264412503262 1204 32-33 -0.16757933542830727 1204 34-35 -0.11102774160217876 1204 36-37 -0.1947924233154552 1204 38-39 -0.11043186173483832 1204 40-41 -0.008744541804709627 1204 42-43 -0.13301501296137275 1204 44-45 -0.07531339215039168 1204 46-47 -0.13591282347611156 1204 48-49 -0.03755203637655313 1204 50-51 -0.031577875907117914 1204 52-53 -0.02532157456093387 1204 54-55 -0.1208059113992519 1204 56-57 0.11207580608593304 1204 58-59 -0.09519432898778035 1204 60-61 -0.14760088060041454 1204 62-63 -0.04137293022078126 1204 64-65 0.013976809073376728 1204 66-67 -0.12065561594581453 1204 68-69 -0.027641284387001974 1204 70-71 -0.06347165873704341 1204 72-73 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46-47 0.13815960413855777 2401 48-49 0.14001794839828108 2401 50-51 0.06149283540158734 2401 52-53 0.0880606648258464 2401 54-55 0.054061357661304044 2401 56-57 0.06077194023797006 2401 58-59 0.06678115394070971 2401 60-61 0.07331897086603334 2401 62-63 0.05362314745889307 2401 64-65 0.1468590133386698 2401 66-67 0.2569416382101224 2401 68-69 0.05162283742780005 2401 70-71 0.027690129327410773 2401 72-73 -0.008754386541944825 2401 74-75 0.046654789968350485 2401 76-77 0.25473310515847913 2401 78-79 -0.08130777903813424 2401 80-81 0.1789998566301918 2401 82-83 0.3472782037527651 2401 84-85 0.04476863142591725 2401 86-87 0.2260407966229181 2401 88-89 0.11063085156113317 2401 90-91 0.39941266882953386 2402 1 0.3250252737279169 2402 2 0.23929752618641942 2402 3 0.29952604704838137 2402 4 0.3786999493583352 2402 5 0.30491660941648746 2402 6 0.3168665441260572 2402 7 0.1737075771683223 2402 8 0.19814857999430302 2402 9 0.030242924949163807 2402 10-11 0.1515591162822716 2402 12-13 0.128559381073039 2402 14-15 0.17358372913266606 2402 16-17 0.1068084454010858 2402 18-19 0.11166154944258722 2402 20-21 0.08129959090583583 2402 22-23 0.14554769834956716 2402 24-25 0.026164411013091637 2402 26-27 0.1265779325331735 2402 28-29 0.15266022755130138 2402 30-31 0.08546684769694934 2402 32-33 0.10874080973941602 2402 34-35 0.11461983260088715 2402 36-37 0.09651784967487487 2402 38-39 0.10680272269257785 2402 40-41 0.04206445053269903 2402 42-43 0.06368187030032857 2402 44-45 0.11678339247208669 2402 46-47 0.05617284993787308 2402 48-49 0.06691575289692508 2402 50-51 0.055329985268414816 2402 52-53 0.11886817163350827 2402 54-55 0.08205439415090154 2402 56-57 0.1600292038171247 2402 58-59 0.06711407646439937 2402 60-61 0.08119899915048023 2402 62-63 0.14298570261257026 2402 64-65 0.09646057086449389 2402 66-67 0.15245766427419483 2402 68-69 0.21989117217214016 2402 70-71 0.2483222306430548 2402 72-73 0.1541261442966615 2402 74-75 0.23693953385097188 2402 76-77 0.059730555157500476 2402 78-79 0.25563279766452496 2402 80-81 0.20200425723862026 2402 82-83 0.32460700738741366 2402 84-85 0.07528789499658473 2402 86-87 0.2764458775026313 2402 88-89 0.642357759191654 2402 90-91 0.577190446607311 2403 1 -0.21744861278615701 2403 2 -0.1245736174489025 2403 3 -0.08512816037129056 2403 4 -0.024895728474774614 2403 5 0.09038004268334277 2403 6 0.19719478911636656 2403 7 0.06211257629091449 2403 8 0.11504451456518439 2403 9 0.07046923595433441 2403 10-11 0.12763410815507825 2403 12-13 0.06961248615430549 2403 14-15 0.1453309967193377 2403 16-17 0.05614147186931007 2403 18-19 0.12342894045387709 2403 20-21 0.05382267953539355 2403 22-23 0.0834497981103226 2403 24-25 0.057320683379238346 2403 26-27 0.06745948310405225 2403 28-29 0.055007655034032155 2403 30-31 0.11566930114793905 2403 32-33 0.08472909994436861 2403 34-35 0.04183408440389513 2403 36-37 0.02448742548819638 2403 38-39 0.10415304699348837 2403 40-41 0.088744207008709 2403 42-43 0.05431920598248752 2403 44-45 0.10389045704371114 2403 46-47 0.07410908420163054 2403 48-49 0.08102533266887946 2403 50-51 0.1468265581327799 2403 52-53 0.1653390472334877 2403 54-55 0.048201475840357944 2403 56-57 0.04492311108713665 2403 58-59 -0.035252129197530735 2403 60-61 0.0010889031538496852 2403 62-63 0.18651568223091886 2403 64-65 0.14271753658026398 2403 66-67 0.1489909190050298 2403 68-69 0.028371377614206494 2403 70-71 0.2064187570136653 2403 72-73 0.12925855510199824 2403 74-75 0.2555756540255416 2403 76-77 0.1450168613104168 2403 78-79 0.17997408170647589 2403 80-81 0.16117969563633494 2403 82-83 0.07708332861120937 2403 84-85 0.2756226661362149 2403 86-87 -0.12630852112805258 2403 88-89 -0.19755820719490202 2403 90-91 -0.8394762200593604 2404 1 -0.47050534837828906 2404 2 -0.28468002733549014 2404 3 -0.44059147548951927 2404 4 -0.4271954578933901 2404 5 -0.15773347561227524 2404 6 -0.23951515435386028 2404 7 -0.08929560926914348 2404 8 0.012242122389380938 2404 9 -0.03821628375153807 2404 10-11 0.03900624966370003 2404 12-13 0.04770358021522014 2404 14-15 0.06621345426408709 2404 16-17 0.06810293014351032 2404 18-19 0.015195033327358942 2404 20-21 -0.0224245454317753 2404 22-23 0.02103921798027386 2404 24-25 0.0261841295597236 2404 26-27 -0.03229380011361371 2404 28-29 0.0022732460943046817 2404 30-31 -0.01675628897010739 2404 32-33 0.04940296027427138 2404 34-35 0.04998124082803912 2404 36-37 0.025447351153935927 2404 38-39 0.016910390514624396 2404 40-41 -0.07100964321280401 2404 42-43 -0.04525998406308673 2404 44-45 -0.04685674747237556 2404 46-47 -0.02085931175302136 2404 48-49 0.008822577289485878 2404 50-51 0.009217583470196189 2404 52-53 -0.06238603415026489 2404 54-55 0.02861867889807712 2404 56-57 0.010248912269261723 2404 58-59 -0.014161684403859454 2404 60-61 0.0340624036956072 2404 62-63 0.0398075107708209 2404 64-65 -0.07549461013127257 2404 66-67 0.036811551840720824 2404 68-69 0.02860518701844228 2404 70-71 -0.08619263159938839 2404 72-73 -0.11304017868061322 2404 74-75 0.04383562872313718 2404 76-77 0.24780203948295565 2404 78-79 -0.09674881686851222 2404 80-81 -0.12911578438971105 2404 82-83 0.002820712275692472 2404 84-85 -0.25464746655458015 2404 86-87 -0.2451494345987797 2404 88-89 -0.20172826231372198 2404 90-91 0.1669340363509022 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 18.0 31 473.0 32 2999.0 33 5779.0 34 7639.0 35 9846.0 36 13710.0 37 21376.0 38 56230.0 39 349329.0 40 346550.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.004614613433983 14.083785457333256 24.137155746889192 32.77444418234358 2 28.951187116259124 17.55233989012893 25.228693281301645 28.267779712310304 3 26.7866752176445 19.12875856019225 19.402119561344666 34.682446660818584 4 26.62906398312425 20.644168123555655 18.451893177582377 34.27487471573772 5 27.17381555846865 21.121470755538738 18.55877948126971 33.1459342047229 6 27.220624388014482 24.09413857624986 22.01304995767548 26.672187078060173 7 25.313625300848088 23.606024456077716 22.370811930477217 28.709538312596983 8 24.590484170384137 25.16668734773309 23.23892528893088 27.003903192951896 9 24.362951487132488 25.21718191188883 23.800385527840195 26.61948107313849 10-11 24.60172566094436 24.10280005258315 23.424624884360075 27.87084940211242 12-13 24.727286353321894 23.764142470842767 23.35453449786166 28.154036677973682 14-15 24.99450211254022 23.199856502065856 23.32572433899421 28.47991704639971 16-17 25.205694705687947 23.20698225564501 22.938046486942056 28.649276551724984 18-19 25.178174553933967 23.383651801279935 22.942715084114607 28.495458560671494 20-21 25.35926698110078 23.56744710049401 22.7614383702173 28.311847548187906 22-23 25.21110044978248 23.170432054096757 22.933623605410165 28.6848438907106 24-25 25.119878518187257 23.4129533914287 22.972815250095522 28.494352840288517 26-27 25.390165722913842 23.5452712639244 22.87108897486206 28.193474038299698 28-29 25.462528979088372 23.069750070336102 22.864086079103235 28.603634871472288 30-31 25.528903566321194 23.335274489279414 22.897101534741775 28.23872040965762 32-33 25.65377445681971 23.303037423437818 22.814417970769302 28.228770148973165 34-35 25.569142742058336 23.04966782255295 22.88351454841683 28.49767488697188 36-37 25.533862355443578 23.29319601724596 22.93461730017131 28.238324327139154 38-39 25.890080724502933 23.312554209800506 22.713308415025033 28.084056650671524 40-41 25.8817432634222 23.01249091116723 22.750197677595434 28.35556814781514 42-43 25.872570344877733 23.249027532052057 22.776577761081892 28.10182436198832 44-45 25.99572086243043 23.251522538838 22.706462805554427 28.046293793177146 46-47 25.9576497600547 22.997775704958027 22.709166037082838 28.335408497904435 48-49 25.98414094067627 23.2143290166539 22.75233444670672 28.049195595963116 50-51 26.178311105819223 23.2698979381176 22.48750992628384 28.06428102977933 52-53 26.186443389575793 22.984989281006893 22.508440497460366 28.320126831956948 54-55 26.379825400968706 23.228743471499932 22.421563181155975 27.969867946375388 56-57 26.462149609112434 23.136910565167103 22.367609923881385 28.03332990183908 58-59 26.474707677169256 23.014182005266505 22.314273659930777 28.196836657633455 60-61 26.397735829309205 23.354129901996412 22.287457808452555 27.960676460241825 62-63 26.656339926683366 23.136562289383562 22.13557626265307 28.07152152128 64-65 26.800421743792434 22.94529682277989 21.96934302126799 28.284938412159683 66-67 26.698881119442202 23.034537263255867 22.159371276582938 28.107210340718986 68-69 26.790216757332097 22.949815151453258 22.01365768636342 28.246310404851226 70-71 26.85900840793377 22.772674862998613 21.967119667310108 28.401197061757504 72-73 26.828954724980342 23.173287648706747 21.716940684731227 28.280816941581683 74-75 27.26306924015111 22.949931678950477 21.619156453456068 28.167842627442347 76-77 27.44373210222917 22.66919739246833 21.2311594232929 28.655911082009606 78-79 27.425041449431692 22.8171630610655 21.122230878328438 28.63556461117437 80-81 27.622526749123814 22.70662191481076 20.788596930418283 28.88225440564714 82-83 28.165093484106517 22.077389542081647 20.418253649073833 29.339263324738003 84-85 28.519402102774144 21.62204957142254 19.963687032892793 29.894861292910523 86-87 29.370277078085643 20.79688573391344 18.778719181741852 31.054118006259063 88-89 28.33578269903208 20.610145441645976 18.798459433436378 32.25561242588557 90-91 25.993864646591124 22.48794841294685 21.323483378200713 30.194703562261317 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 1.5 15 2.0 16 5.0 17 12.5 18 17.5 19 27.5 20 49.0 21 70.0 22 89.0 23 118.0 24 183.5 25 273.5 26 383.0 27 531.5 28 745.5 29 1056.0 30 1436.0 31 1929.0 32 2569.5 33 3306.0 34 4313.5 35 5581.5 36 7155.0 37 9014.5 38 11299.5 39 14192.5 40 17557.0 41 21442.5 42 25542.0 43 29656.0 44 34036.0 45 38894.5 46 43922.0 47 48901.0 48 53463.0 49 57661.0 50 61307.0 51 64348.0 52 66437.0 53 67262.5 54 67445.5 55 66817.0 56 64985.0 57 62502.0 58 59598.5 59 56224.5 60 52913.0 61 49388.0 62 46027.0 63 42598.5 64 38763.5 65 35185.5 66 31592.0 67 28126.5 68 24840.0 69 21379.5 70 18080.0 71 15022.0 72 12189.0 73 9655.5 74 7474.0 75 5780.0 76 4375.0 77 3161.5 78 2324.0 79 1687.5 80 1163.0 81 807.0 82 552.5 83 355.0 84 222.0 85 142.0 86 92.0 87 57.0 88 38.0 89 24.0 90 12.5 91 7.5 92 4.5 93 3.0 94 3.0 95 2.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0015971516642934631 2 0.010565772548402911 3 8.600047423118647E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 10479.0 32-33 22764.0 34-35 25116.0 36-37 27269.0 38-39 28880.0 40-41 31309.0 42-43 32645.0 44-45 34349.0 46-47 35442.0 48-49 35862.0 50-51 36342.0 52-53 36320.0 54-55 35398.0 56-57 35189.0 58-59 34160.0 60-61 33409.0 62-63 32059.0 64-65 30279.0 66-67 29263.0 68-69 27874.0 70-71 26067.0 72-73 24572.0 74-75 22896.0 76-77 20929.0 78-79 19606.0 80-81 17916.0 82-83 16343.0 84-85 14998.0 86-87 13402.0 88-89 12066.0 90-91 10746.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.80340719323004 #Duplication Level Percentage of deduplicated Percentage of total 1 99.80937213071105 99.61315408461982 2 0.1851805774441554 0.3696330514987302 3 0.004542397588856088 0.013600402685824516 4 9.048942559225715E-4 0.0036124611956262127 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.371469219815983E-4 0.0 0.0 0.0 0.0 2 7.371469219815983E-4 0.0 0.0 0.0 0.0 3 7.371469219815983E-4 0.0 0.0 0.0 1.228578203302664E-4 4 7.371469219815983E-4 0.0 0.0 0.0 1.228578203302664E-4 5 7.371469219815983E-4 0.0 0.0 0.0 1.228578203302664E-4 6 7.371469219815983E-4 0.0 0.0 0.0 2.457156406605328E-4 7 8.600047423118648E-4 0.0 0.0 0.0 3.6857346099079916E-4 8 0.0029485876879263933 0.0 0.0 0.0 3.6857346099079916E-4 9 0.0030714455082566598 0.0 0.0 0.0 3.6857346099079916E-4 10-11 0.0030714455082566598 0.0 0.0 0.0 3.6857346099079916E-4 12-13 0.0031943033285869262 0.0 0.0 0.0 3.6857346099079916E-4 14-15 0.0031943033285869262 0.0 0.0 0.0 3.6857346099079916E-4 16-17 0.0031943033285869262 0.0 0.0 0.0 3.6857346099079916E-4 18-19 0.0033171611489171927 0.0 0.0 0.0 3.6857346099079916E-4 20-21 0.003501447879412592 0.0 0.0 0.0 3.6857346099079916E-4 22-23 0.0036243056997428587 1.228578203302664E-4 0.0 0.0 3.6857346099079916E-4 24-25 0.0036857346099079917 1.228578203302664E-4 0.0 0.0 3.6857346099079916E-4 26-27 0.0036857346099079917 1.228578203302664E-4 0.0 0.0 3.6857346099079916E-4 28-29 0.004054308070898792 1.228578203302664E-4 0.0 0.0 3.6857346099079916E-4 30-31 0.004300023711559324 1.228578203302664E-4 0.0 0.0 3.6857346099079916E-4 32-33 0.004361452621724457 1.228578203302664E-4 1.228578203302664E-4 0.0 3.6857346099079916E-4 34-35 0.0047300260827152565 1.228578203302664E-4 1.228578203302664E-4 0.0 4.3000237115593234E-4 36-37 0.0050985995437060555 1.228578203302664E-4 1.228578203302664E-4 0.0 4.914312813210656E-4 38-39 0.0054671730046968545 2.457156406605328E-4 1.228578203302664E-4 0.0 4.914312813210656E-4 40-41 0.0056514597351922545 2.457156406605328E-4 1.228578203302664E-4 0.0 6.14289101651332E-4 42-43 0.0057128886453573875 2.457156406605328E-4 1.228578203302664E-4 0.0 6.14289101651332E-4 44-45 0.0058357464656876535 2.457156406605328E-4 1.228578203302664E-4 0.0 6.14289101651332E-4 46-47 0.0061428910165133195 2.457156406605328E-4 1.228578203302664E-4 0.0 6.14289101651332E-4 48-49 0.0063271777470087194 2.457156406605328E-4 1.842867304953996E-4 0.0 6.757180118164652E-4 50-51 0.0066957512079995184 2.457156406605328E-4 3.07144550825666E-4 0.0 7.371469219815983E-4 52-53 0.006880037938494918 2.457156406605328E-4 3.6857346099079916E-4 0.0 7.371469219815983E-4 54-55 0.006941466848660051 2.457156406605328E-4 3.6857346099079916E-4 0.0 7.371469219815983E-4 56-57 0.007002895758825184 2.457156406605328E-4 3.6857346099079916E-4 0.0 7.371469219815983E-4 58-59 0.007002895758825184 2.457156406605328E-4 3.6857346099079916E-4 0.0 7.371469219815983E-4 60-61 0.007248611399485717 2.457156406605328E-4 3.6857346099079916E-4 0.0 7.371469219815983E-4 62-63 0.007248611399485717 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