##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-5A_S59_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1250126
Sequences flagged as poor quality	0
Sequence length	61
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.37323917749091	40.0	40.0	40.0	40.0	40.0
2	39.24220118612044	40.0	40.0	40.0	40.0	40.0
3	39.18519573227019	40.0	40.0	40.0	40.0	40.0
4	39.07681945659878	40.0	40.0	40.0	40.0	40.0
5	39.10183773475634	40.0	40.0	40.0	40.0	40.0
6	39.14990968910334	40.0	40.0	40.0	40.0	40.0
7	39.18846260296962	40.0	40.0	40.0	40.0	40.0
8	38.91208726160403	40.0	40.0	40.0	40.0	40.0
9	39.21692693376507	40.0	40.0	40.0	40.0	40.0
10	39.21899712508979	40.0	40.0	40.0	40.0	40.0
11	39.22023859994913	40.0	40.0	40.0	40.0	40.0
12	39.21942908154858	40.0	40.0	40.0	40.0	40.0
13	39.19620262277562	40.0	40.0	40.0	40.0	40.0
14	39.237382471846836	40.0	40.0	40.0	40.0	40.0
15	39.22167525513428	40.0	40.0	40.0	40.0	40.0
16	39.215739853422775	40.0	40.0	40.0	40.0	40.0
17	39.226327586179316	40.0	40.0	40.0	40.0	40.0
18	39.226929125544146	40.0	40.0	40.0	40.0	40.0
19	39.23939826865452	40.0	40.0	40.0	40.0	40.0
20	39.018159769495234	40.0	40.0	40.0	40.0	40.0
21	39.07959357696744	40.0	40.0	40.0	40.0	40.0
22	39.0941713075322	40.0	40.0	40.0	40.0	40.0
23	39.2112667043162	40.0	40.0	40.0	40.0	40.0
24	39.23669774086772	40.0	40.0	40.0	40.0	40.0
25	39.069936950355405	40.0	40.0	40.0	40.0	40.0
26	39.22087533576615	40.0	40.0	40.0	40.0	40.0
27	39.22648757005294	40.0	40.0	40.0	40.0	40.0
28	39.03701546884074	40.0	40.0	40.0	40.0	40.0
29	39.189843263799006	40.0	40.0	40.0	40.0	40.0
30	39.025026277351245	40.0	40.0	40.0	40.0	40.0
31	39.06473107510763	40.0	40.0	40.0	40.0	40.0
32	39.02695568286717	40.0	40.0	40.0	40.0	40.0
33	39.104533463026925	40.0	40.0	40.0	40.0	40.0
34	38.99335586972833	40.0	40.0	40.0	40.0	40.0
35	39.134263266262764	40.0	40.0	40.0	40.0	40.0
36	38.9693934851367	40.0	40.0	40.0	40.0	40.0
37	38.99634276864892	40.0	40.0	40.0	40.0	40.0
38	38.97001102288889	40.0	40.0	40.0	40.0	40.0
39	39.035050866872616	40.0	40.0	40.0	40.0	40.0
40	38.88262463143715	40.0	40.0	40.0	40.0	40.0
41	38.807241830023536	40.0	40.0	40.0	40.0	40.0
42	38.7845017222264	40.0	40.0	40.0	40.0	40.0
43	38.72019460438388	40.0	40.0	40.0	40.0	40.0
44	38.56170018062179	40.0	40.0	40.0	40.0	40.0
45	38.55181797674794	40.0	40.0	40.0	40.0	40.0
46	38.308373715929434	40.0	40.0	40.0	40.0	40.0
47	38.17432482805733	40.0	40.0	40.0	40.0	40.0
48	38.10291602606457	40.0	40.0	40.0	40.0	40.0
49	37.83305362819428	40.0	40.0	40.0	40.0	40.0
50	37.75744204984137	40.0	40.0	40.0	24.0	40.0
51	37.47466095417582	40.0	40.0	40.0	24.0	40.0
52	37.357235990612146	40.0	40.0	40.0	24.0	40.0
53	36.98466394587425	40.0	40.0	40.0	24.0	40.0
54	36.81032151959082	40.0	40.0	40.0	24.0	40.0
55	36.638487640445845	40.0	40.0	40.0	24.0	40.0
56	36.2755242271579	40.0	40.0	40.0	24.0	40.0
57	36.08052148343447	40.0	40.0	40.0	24.0	40.0
58	35.80669148549826	40.0	40.0	40.0	24.0	40.0
59	35.45507732820532	40.0	40.0	40.0	12.0	40.0
60	35.14257602833634	40.0	40.0	40.0	12.0	40.0
61	34.70025101469772	40.0	40.0	40.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.04646670110643214
1101	2	0.057527355152110715
1101	3	0.07193296875974653
1101	4	0.03654210063231034
1101	5	0.08529697110071055
1101	6	0.11264115166348887
1101	7	0.10595304646795256
1101	8	0.4538661840654896
1101	9	0.04841276313612752
1101	10	0.10189779428709755
1101	11	0.025439826673427035
1101	12	0.03750406236503068
1101	13	0.07175092639156588
1101	14	0.09648127532443596
1101	15	0.12897011986668616
1101	16	0.04813211290506558
1101	17	0.053743819169319806
1101	18	0.11284523673027991
1101	19	0.08516075116157396
1101	20	0.13398111757126685
1101	21	0.03717298725585749
1101	22	0.026543822634181424
1101	23	0.08226156373494575
1101	24	0.04193369845616246
1101	25	0.02036804314560925
1101	26	0.06605179123670979
1101	27	0.04885219790481443
1101	28	0.10268739161050178
1101	29	0.07885055994256618
1101	30	0.10765762060376716
1101	31	0.14176283849002402
1101	32	0.11100198813883821
1101	33	0.10853813866717843
1101	34	0.08488938617604447
1101	35	0.2165411151945591
1101	36	0.05118056369882851
1101	37	0.17454092323870185
1101	38	0.11855214416292625
1101	39	0.10897937343825248
1101	40	0.10598410845236828
1101	41	0.11444409891591789
1101	42	0.1255200124545226
1101	43	0.03929528613280553
1101	44	0.09514734205951925
1101	45	0.052178149054469714
1101	46	0.06711969808699081
1101	47	0.24172234855772246
1101	48	-0.0032756894240080214
1101	49	0.09346852439544051
1101	50	0.1943998973817216
1101	51	0.09769707965246255
1101	52	0.14531184971018973
1101	53	0.20299813506233022
1101	54	0.13484160523972832
1101	55	0.32421500280414506
1101	56	0.016528896494151013
1101	57	0.17807423476102002
1101	58	-0.07661046286273177
1101	59	0.10393217753128425
1101	60	0.08696832796933762
1101	61	0.42880444358149106
1102	1	0.09656833632449491
1102	2	0.01386500428834836
1102	3	0.058379503082413464
1102	4	0.01970273980315085
1102	5	0.007242133198076317
1102	6	0.020668898409283543
1102	7	-0.03722127236470385
1102	8	0.33587481020278886
1102	9	-0.010346432224650925
1102	10	-2.3258037295192935E-5
1102	11	0.04909024569194287
1102	12	-0.02635875738050686
1102	13	-0.018189568972459824
1102	14	-0.022536073174727278
1102	15	0.0039766268552696715
1102	16	-0.15751383940099117
1102	17	-0.11970697332667868
1102	18	-0.08860595580748765
1102	19	-0.021781140878260885
1102	20	0.07999238877970782
1102	21	-0.0672038419966583
1102	22	0.008690601998210923
1102	23	0.04766337698421097
1102	24	-0.028907010778780773
1102	25	-0.0679525985365359
1102	26	-0.08220596059794616
1102	27	-0.06925022856306384
1102	28	-0.024761878329037756
1102	29	-0.006067076567745744
1102	30	0.12100185307725297
1102	31	0.010451966597720741
1102	32	-0.018472982276058758
1102	33	0.12608924573861202
1102	34	0.12529284786242556
1102	35	0.0824955728997665
1102	36	-0.10471735497974777
1102	37	0.09142284371683473
1102	38	-0.003963109007038668
1102	39	-0.08585333622323077
1102	40	-0.044892970510765906
1102	41	0.09920238124760772
1102	42	0.029777270862261673
1102	43	6.419125471097686E-4
1102	44	0.008975820679843594
1102	45	0.012139394219182975
1102	46	0.07491836728556933
1102	47	-0.04912391961727991
1102	48	-0.092585456469088
1102	49	-0.10173318278597066
1102	50	0.05657225670378807
1102	51	0.01537555018045822
1102	52	-0.03375063283925073
1102	53	-0.025929238742314453
1102	54	0.07137377941943157
1102	55	-0.017808344113156238
1102	56	-0.06234444958710128
1102	57	-0.1704176740155745
1102	58	-0.05094671059080724
1102	59	0.07389825285334695
1102	60	-0.11974196260043612
1102	61	-0.04861095846838026
1103	1	-0.04654817565410241
1103	2	0.024853385334864697
1103	3	0.06320993314847811
1103	4	-0.029642778905426326
1103	5	-0.014423418962998369
1103	6	0.02454412967427544
1103	7	0.027848307897961888
1103	8	0.3883468075677783
1103	9	0.08611375379903308
1103	10	0.0984793073583603
1103	11	0.022756801325307663
1103	12	0.030649150420217097
1103	13	0.04714192063888589
1103	14	-8.692371442933222E-4
1103	15	0.11177774445070554
1103	16	0.035428871973174125
1103	17	0.05133960295002282
1103	18	5.253903828119633E-4
1103	19	0.0266677234718955
1103	20	-0.0010870423557562958
1103	21	0.06872608287323345
1103	22	-0.03664719012780182
1103	23	0.04458619891108384
1103	24	0.06727585946394754
1103	25	-0.006600333608034248
1103	26	-0.010481058366202944
1103	27	-0.06051555178455459
1103	28	-0.01637281918124245
1103	29	-0.0520144498641244
1103	30	0.10793719850776284
1103	31	6.093105647408947E-4
1103	32	0.018370730188820517
1103	33	0.2125224120897613
1103	34	-0.05557045028625396
1103	35	0.14216211736346196
1103	36	0.07976080572846911
1103	37	0.034697900722235886
1103	38	-0.02277205185039577
1103	39	0.055310153690591335
1103	40	-0.053404254021280906
1103	41	0.08859813391248395
1103	42	0.03092325264967144
1103	43	-0.034908943475990384
1103	44	0.059664780282830066
1103	45	-0.012026621900176337
1103	46	0.08591727606081179
1103	47	0.02014648117912543
1103	48	0.1271629064209563
1103	49	0.031508833199616504
1103	50	0.04704363511535803
1103	51	0.20470802092476958
1103	52	0.1312404452316258
1103	53	0.1420393891396401
1103	54	-0.04169608124030333
1103	55	0.0020479793510546074
1103	56	0.23295115138219558
1103	57	0.020148595180117468
1103	58	0.21839855262776098
1103	59	-0.038340430785133606
1103	60	-7.87101585395078E-4
1103	61	0.26419148504313483
1104	1	0.028355260938596416
1104	2	0.039489317775107224
1104	3	-0.01317490032421631
1104	4	0.04647332043938235
1104	5	0.013814336321502196
1104	6	-0.013830586992099825
1104	7	-0.008999533805237547
1104	8	0.41295949450595515
1104	9	0.08600083378568257
1104	10	0.008267327811090297
1104	11	0.04757749881439821
1104	12	-0.04161474856056202
1104	13	-0.10423213762887684
1104	14	-0.05584526295930914
1104	15	0.06696762061595507
1104	16	-0.125994292288496
1104	17	0.0061775407670126015
1104	18	-0.04235010612933365
1104	19	0.024964620936387405
1104	20	0.022723059773554155
1104	21	0.010524472328931722
1104	22	0.01822289242524988
1104	23	-0.0632565482132037
1104	24	0.026839900357011004
1104	25	-0.012504215679236097
1104	26	-0.03293276115866206
1104	27	-0.01883127097392645
1104	28	-0.05933720467414361
1104	29	-0.0384531955372438
1104	30	-0.08074981822300487
1104	31	-0.10157503277824986
1104	32	-0.022741198609040225
1104	33	-0.02445070106074354
1104	34	-0.030033731385252338
1104	35	0.024983960073377887
1104	36	-0.004746775157919103
1104	37	-0.06712468514089664
1104	38	0.015850210291475264
1104	39	0.062356155805325386
1104	40	0.037877494073548235
1104	41	-0.04131052749181663
1104	42	-0.04557605122557362
1104	43	-0.11053098546259577
1104	44	0.011100130603608704
1104	45	-0.08100524616749283
1104	46	-0.15189899041595112
1104	47	-0.12476788048946474
1104	48	-0.0646719171809309
1104	49	-0.017692509644149368
1104	50	0.07431394753945142
1104	51	0.024823855669815487
1104	52	-0.10343369913441336
1104	53	-0.1331815671610883
1104	54	-0.16695435024109173
1104	55	0.011478221656211929
1104	56	-0.10149415553802044
1104	57	-0.21047670779177707
1104	58	-0.01284096286527614
1104	59	-0.12132346229213908
1104	60	0.032032053783240144
1104	61	-0.21177231440265132
1201	1	-0.06101649231479911
1201	2	-0.06796903994933956
1201	3	-0.060876746134198356
1201	4	-0.0638792214894437
1201	5	-0.0793358214872697
1201	6	-0.06352909241409321
1201	7	-0.19728013458384197
1201	8	0.23065147470924074
1201	9	-0.028586550518589604
1201	10	-0.10724214276197586
1201	11	-0.047750460969950836
1201	12	-0.1176239264403165
1201	13	-0.013344724259958696
1201	14	-0.18848547942955207
1201	15	-0.07489345854223473
1201	16	-0.031043968279050205
1201	17	-0.08100719988215133
1201	18	-0.05872865068626254
1201	19	-0.01272629492758881
1201	20	-0.034961060693284196
1201	21	-0.0775291376234577
1201	22	-0.08199591117548977
1201	23	-0.038135070255258086
1201	24	-0.14431097792760283
1201	25	-0.023687965674113798
1201	26	0.011356035032640932
1201	27	0.01330154219768076
1201	28	-0.11796183266709193
1201	29	-0.10131266590562404
1201	30	0.04974022815763135
1201	31	0.014725528456061454
1201	32	-0.02375334435802756
1201	33	0.06746830271765702
1201	34	-0.06337109010444664
1201	35	0.04098896246814121
1201	36	-0.04311211372016288
1201	37	-0.04481756593899178
1201	38	-0.03570884211904257
1201	39	-0.029754224368488735
1201	40	-0.05709432140276505
1201	41	-6.302509273368173E-4
1201	42	0.02951718080448984
1201	43	0.054651405452858626
1201	44	0.09342779974144833
1201	45	0.11569591600916596
1201	46	0.07342164180160182
1201	47	0.10572093780734804
1201	48	-0.1223468270191077
1201	49	-0.1965086777770111
1201	50	-0.07721522149810056
1201	51	-0.08791935609686163
1201	52	0.014630185785946992
1201	53	0.07019730079124287
1201	54	0.06292758996760028
1201	55	0.11293534310961206
1201	56	-0.005611511227932908
1201	57	-0.03137398676739167
1201	58	-0.007605232175869503
1201	59	-0.035726019981375146
1201	60	-0.009610221774202898
1201	61	0.035422537469969484
1202	1	-0.12270012210452563
1202	2	-0.139228106854425
1202	3	-0.09168006914620008
1202	4	-0.08759177134160012
1202	5	-0.12446639163730566
1202	6	-0.05885633283542546
1202	7	-0.03506965683499175
1202	8	0.23960742501183319
1202	9	0.027345513633171947
1202	10	-0.14935226261381018
1202	11	-0.112714942520455
1202	12	-0.04759918313467182
1202	13	-0.09649855797398743
1202	14	-0.09138828406697286
1202	15	-0.07983281961831779
1202	16	-0.080624613423673
1202	17	-0.16485543749642062
1202	18	-0.0041440607694767095
1202	19	-0.15681872091338533
1202	20	-0.07030590921351632
1202	21	-0.045369556993811955
1202	22	-0.09970682731847091
1202	23	0.006208095472189257
1202	24	-0.1274287997727015
1202	25	-0.012425602417174275
1202	26	-0.08977014962609786
1202	27	-0.03866146632788059
1202	28	-0.06719853087987815
1202	29	-0.05266254265410453
1202	30	-0.03478071323499421
1202	31	-0.07367715140566133
1202	32	0.008438786454441072
1202	33	-0.09969341950734645
1202	34	-0.10032381432224469
1202	35	-0.05971200313561553
1202	36	-0.022159612703610776
1202	37	-0.11115793855074685
1202	38	-0.08693662984845218
1202	39	-0.11401269762024668
1202	40	0.05403251942632181
1202	41	-0.043059672572589136
1202	42	-0.0762745300589529
1202	43	-0.10232572623536385
1202	44	-0.12943090198159268
1202	45	-0.18484353868743852
1202	46	-0.010355812081762394
1202	47	-0.19176603307780482
1202	48	-0.05712504402342944
1202	49	-0.05901360551302304
1202	50	-0.17638374516130995
1202	51	-0.30090036900688943
1202	52	-0.0015116048771162127
1202	53	-0.21682607799006348
1202	54	-0.23016923959292512
1202	55	-0.012783796346219845
1202	56	-0.02930752765389144
1202	57	-0.1422698762993022
1202	58	0.057989586248361036
1202	59	-0.0919221022827088
1202	60	-0.11692042449984541
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2403	7	0.03155015311468645
2403	8	0.43088985681018244
2403	9	0.03672782071956249
2403	10	0.0991582746004056
2403	11	0.2491958978919655
2403	12	0.12017422874566108
2403	13	0.14280665741355136
2403	14	0.13462815755040936
2403	15	0.08181371758546163
2403	16	0.19341382866227264
2403	17	0.12861718689087098
2403	18	0.11150432068335192
2403	19	0.02425547258658156
2403	20	-0.09558217132659763
2403	21	0.08872262021854738
2403	22	0.11919522452281228
2403	23	0.10142538207671237
2403	24	0.08940033179221984
2403	25	0.07465975968801075
2403	26	0.056553849562071434
2403	27	0.06575722650023152
2403	28	0.0566911509186383
2403	29	0.16313469697623617
2403	30	0.09012572016614229
2403	31	0.11968002795050126
2403	32	0.07509723035461491
2403	33	0.2080928224991112
2403	34	0.0981690700480371
2403	35	0.14384192874985757
2403	36	0.034308865712603165
2403	37	0.11083587349116186
2403	38	0.08295120770100795
2403	39	0.18562439123326868
2403	40	0.04817216680016401
2403	41	0.11459921444333787
2403	42	0.190723657669416
2403	43	0.06353817653759819
2403	44	0.05078942900433958
2403	45	0.009700851525906273
2403	46	0.036055570315383534
2403	47	0.08154697827549029
2403	48	0.02204531799365128
2403	49	0.08254249431136174
2403	50	0.08782783836731767
2403	51	0.18411181178173308
2403	52	0.23211885747077332
2403	53	0.30302713910206336
2403	54	-0.009628638378174514
2403	55	0.1852661027943654
2403	56	0.04725843622728121
2403	57	0.019176965146833425
2403	58	0.16325326622350644
2403	59	0.004278783818271847
2403	60	0.07540773436400627
2403	61	0.2800281416446282
2404	1	-0.09391526404979089
2404	2	0.06337844633421241
2404	3	0.015642216553729327
2404	4	-0.05325622094244409
2404	5	0.019455804430613455
2404	6	0.010577058856441113
2404	7	-0.009497310403098425
2404	8	0.2568443090012096
2404	9	-0.034979620186575744
2404	10	-0.042074478786105374
2404	11	-0.049397678725924266
2404	12	-0.08969167163748182
2404	13	0.0016573891515037076
2404	14	-0.12841279465108357
2404	15	-0.03589376519409626
2404	16	-0.045943519075514416
2404	17	-0.12321948584744291
2404	18	-0.05323067293095107
2404	19	-0.016720020996316975
2404	20	-0.05556060651701955
2404	21	-0.006872819766371663
2404	22	-0.11354272116737008
2404	23	-0.05520501174277115
2404	24	-0.08666560837067294
2404	25	0.03947195741428544
2404	26	0.05249814744955472
2404	27	0.007447185254918054
2404	28	-0.04421724051406528
2404	29	-0.003600537410207494
2404	30	-0.017897226466182303
2404	31	0.045704618114434936
2404	32	0.03464829498623345
2404	33	0.02176370791250548
2404	34	-0.06318150466460537
2404	35	-0.02209326166950376
2404	36	0.04578584580179523
2404	37	0.11654243505555684
2404	38	0.056734261529285845
2404	39	-0.06964590167127938
2404	40	0.08513723390230865
2404	41	0.09209774887375488
2404	42	0.05052386913016704
2404	43	0.09706674467611265
2404	44	-0.02482689534714666
2404	45	0.03738676949588182
2404	46	0.055984961266538846
2404	47	-0.11907248658224745
2404	48	-0.05085446845192365
2404	49	-0.0011041045203654676
2404	50	0.021391998909997767
2404	51	-0.0540322537082929
2404	52	0.03367471660860133
2404	53	0.07101883769720985
2404	54	0.013128778450749223
2404	55	-0.047018824869304865
2404	56	-0.12499335909546261
2404	57	-0.07891020483830147
2404	58	-0.3265065897319346
2404	59	-0.11709236121030386
2404	60	-0.07123584958172557
2404	61	-0.1438506976242664
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	6.0
14	40.0
15	165.0
16	515.0
17	903.0
18	1113.0
19	1151.0
20	1208.0
21	1255.0
22	1380.0
23	1758.0
24	2351.0
25	3532.0
26	5874.0
27	5718.0
28	4798.0
29	4694.0
30	5415.0
31	6970.0
32	10122.0
33	13679.0
34	22004.0
35	36563.0
36	60185.0
37	77186.0
38	149257.0
39	415514.0
40	416770.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.03291606639048	26.91191798540779	22.313155787569023	21.74201016063271
2	31.03031214453583	17.245701633275363	21.86379612935016	29.86019009283864
3	33.190600597381696	17.78618406843492	18.180145617787705	30.843069716395682
4	31.631931501304667	18.597885333158416	20.39666401626716	29.373519149269754
5	28.143803104647052	19.317652780599715	21.711011530037773	30.82753258471546
6	26.658352838033927	20.02654132463448	24.383302163141956	28.931803674189645
7	27.586819248619737	22.37382471846838	24.811738976711148	25.227617056200735
8	26.00513868202085	24.50536985871824	26.015777609616954	23.473713849643957
9	25.669252539344033	24.227957821851557	26.13496559546798	23.96782404333643
10	27.4644315852962	23.740086999230478	24.97156286646306	23.82391854901026
11	26.43709514080981	24.32994754128784	24.85805430812574	24.374903009776613
12	27.48466954530983	23.04463710058026	24.625197780063772	24.845495574046137
13	27.570980845130812	23.58194294015163	23.771203862650644	25.075872352066913
14	28.15772170165247	23.38948234018011	23.92686817168829	24.525927786479123
15	27.45219281896385	23.06943460099222	24.64863541754991	24.82973716249402
16	28.054932062848064	23.545306633091386	23.37420388024887	25.02555742381168
17	28.266990687338716	23.310050346925028	23.78000297570005	24.642955990036207
18	27.482989714636762	22.978243793025662	24.45601483370476	25.082751658632812
19	28.866450261813608	23.346926629795718	23.21853957121122	24.568083537179454
20	27.763521437039145	23.32012933096344	24.18692195826661	24.72942727373081
21	27.75032276746504	23.61353975519268	23.68329272409341	24.952844753248872
22	28.93724312589291	23.548586302500706	23.129588537475424	24.384582034130958
23	28.541123054796074	23.38116317875158	23.599541166250443	24.4781726002019
24	28.784858486264586	23.011520438739772	23.388522436938356	24.815098638057282
25	28.38977831034632	24.387941695477096	22.954886147476333	24.26739384670025
26	27.91206646370046	24.59856046510512	23.241417265139674	24.24795580605475
27	27.96206142420844	24.283552217936432	23.205020933889863	24.549365423965263
28	28.676549403820097	24.77278290348333	22.783943378507445	23.76672431418913
29	28.32724727527247	24.87983201679832	22.985621437856217	23.807299270072992
30	28.816615285179253	24.253795217442082	22.929688687380313	23.999900809998355
31	28.942842561469806	24.644475836835646	23.05703585078624	23.35564575090831
32	29.446391803706184	24.801739984609554	22.484133599333187	23.267734612351074
33	29.007478488047937	24.64541375661037	22.982772040101782	23.36433571523991
34	29.56781956378797	25.391680518603728	21.721410481823433	23.319089435784875
35	29.3619203184319	25.379121784524123	21.783804192537392	23.475153704506585
36	29.674208839748957	25.354324284112163	21.632219472277196	23.33924740386169
37	30.229297070292972	26.270092990700928	21.174922507749226	22.325687431256874
38	30.063929555900764	26.239195089135016	21.384404452031237	22.312470902932986
39	30.468528772299752	25.978981318683076	21.032039970370985	22.520449938646184
40	30.896725610058507	25.886270663917077	20.7351898928588	22.481813833165617
41	30.97642235776422	26.244255574442555	20.744725527447255	22.034596540345966
42	32.02335766423358	25.26139386061394	20.663453654634537	22.051794820517948
43	31.994880511948804	25.524247575242477	21.08941105889411	21.39146085391461
44	32.48168584606672	25.522067375608536	20.608082705263307	21.388164073061436
45	32.391132712541086	25.26783364516931	21.0282508201193	21.3127828221703
46	33.04557544245576	25.703909609039094	19.97056294370563	21.279952004799522
47	33.43910933777875	26.12440665980869	20.02870110692842	20.407782895484136
48	33.24195580441956	26.489031096890308	19.882971702829717	20.386041395860413
49	33.87088000870314	26.242996694727882	19.79931590786194	20.08680738870704
50	33.76630440868971	26.58420538155476	19.45858084250977	20.19090936724576
51	34.22295032660708	26.567641981688244	19.643939890859002	19.565467800845674
52	34.28441840597193	27.281868242627084	19.006636184162108	19.427077167238878
53	34.65048583297137	27.367350840438647	19.02727815947416	18.954885167115822
54	34.972826652118755	27.780088663346458	18.93175226097933	18.31533242355546
55	34.33180147426437	28.16004927546666	19.555640881038943	17.95250836923003
56	34.34616781402135	28.616647015251313	19.294196886383887	17.742988284343447
57	34.69008851185691	28.86278462381461	18.699559640513073	17.74756722381541
58	33.860163600556106	29.44106076386389	19.419366811772967	17.27940882380703
59	33.306322657850465	29.900257814631043	18.810469049845498	17.982950477672997
60	33.16966878326686	30.26170329520893	19.13587357353466	17.432754347989547
61	32.81295370898471	30.68938567953457	18.484249023888243	18.013411587592483
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.0
4	1.0
5	0.0
6	0.5
7	2.0
8	3.0
9	1.5
10	0.0
11	0.0
12	1.5
13	3.0
14	3.0
15	3.0
16	5.5
17	10.5
18	13.0
19	20.0
20	41.0
21	55.0
22	65.0
23	75.0
24	108.0
25	191.5
26	242.0
27	344.5
28	447.0
29	619.5
30	1092.5
31	1393.0
32	1968.0
33	2543.0
34	3369.5
35	5380.5
36	6565.0
37	8540.0
38	10515.0
39	13531.5
40	20570.0
41	24592.0
42	30226.5
43	42657.0
44	49453.0
45	57485.0
46	65517.0
47	74484.0
48	91625.5
49	99800.0
50	106559.5
51	113319.0
52	116734.0
53	119506.5
54	118864.0
55	114031.5
56	109199.0
57	101571.0
58	85573.5
59	77204.0
60	69168.5
61	54067.5
62	47002.0
63	40828.5
64	34655.0
65	29887.0
66	21263.5
67	17408.0
68	14582.5
69	11757.0
70	9561.0
71	5991.5
72	4618.0
73	3532.0
74	2446.0
75	1935.0
76	1101.0
77	778.0
78	567.5
79	357.0
80	285.5
81	156.0
82	98.0
83	76.5
84	41.0
85	27.0
86	23.0
87	19.0
88	16.5
89	14.0
90	14.0
91	15.0
92	16.0
93	19.0
94	26.0
95	30.0
96	33.0
97	36.0
98	48.0
99	297.5
100	535.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.599435576893849E-4
2	0.0
3	3.199677472510771E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	7.999193681276927E-5
30	0.0
31	0.0
32	0.0
33	5.599435576893849E-4
34	0.0
35	0.0
36	0.0
37	7.999193681276927E-5
38	0.0
39	0.0
40	0.0
41	7.999193681276927E-5
42	7.999193681276927E-5
43	7.999193681276927E-5
44	0.0
45	2.3997581043830782E-4
46	7.999193681276927E-5
47	0.0
48	7.999193681276927E-5
49	1.5998387362553855E-4
50	3.199677472510771E-4
51	0.0
52	7.999193681276928E-4
53	3.999596840638464E-4
54	3.199677472510771E-4
55	8.79911304940462E-4
56	3.199677472510771E-4
57	8.79911304940462E-4
58	6.399354945021542E-4
59	2.3997581043830782E-4
60	6.399354945021542E-4
61	0.0010398951785660006
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1250126.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.4429400896596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.08560668056332	65.21121933747354
2	13.473055873829942	21.40678341218617
3	3.039242357241285	7.243390455128257
4	0.8124687860050881	2.5817963636528276
5	0.2571045731404795	1.021257160038831
6	0.10669944481769678	0.5085910561351336
7	0.061831534424287043	0.34384552194442525
8	0.04501131697586508	0.28606650862962624
9	0.025031761614554355	0.17897370644553143
>10	0.09242924860018537	1.0960969389412079
>50	0.0013022372855918643	0.05937880818784323
>100	1.0809275086324602E-4	0.01674505156536294
>500	1.0809275086324602E-4	0.045855679671301594
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.7468847140208267	0.0	0.0	0.0	0.0
2	0.7486445366307076	7.999193681276927E-5	0.0	0.0	0.0
3	0.7504843511774013	7.999193681276927E-5	0.0	0.0	0.0
4	0.7546439318916653	7.999193681276927E-5	0.0	0.0	0.0
5	0.756483746438359	7.999193681276927E-5	0.0	0.0	0.0
6	0.7636030288146954	7.999193681276927E-5	0.0	0.0	0.0
7	0.7677626095289595	7.999193681276927E-5	0.0	0.0	7.999193681276927E-5
8	0.7979195697073735	7.999193681276927E-5	0.0	0.0	7.999193681276927E-5
9	0.8033590214106419	7.999193681276927E-5	0.0	0.0	7.999193681276927E-5
10	0.8077585779353441	7.999193681276927E-5	0.0	0.0	7.999193681276927E-5
11	0.8142379248171784	7.999193681276927E-5	0.0	0.0	7.999193681276927E-5
12	0.8229570459297703	7.999193681276927E-5	0.0	0.0	7.999193681276927E-5
13	0.8415951672071456	7.999193681276927E-5	0.0	0.0	7.999193681276927E-5
14	0.8703922644597425	7.999193681276927E-5	0.0	0.0	7.999193681276927E-5
15	0.8985494262178373	7.999193681276927E-5	0.0	0.0	7.999193681276927E-5
16	0.9351857332780855	7.999193681276927E-5	0.0	0.0	7.999193681276927E-5
17	0.9776614517256661	7.999193681276927E-5	0.0	0.0	7.999193681276927E-5
18	1.0385353156401835	7.999193681276927E-5	0.0	0.0	7.999193681276927E-5
19	1.134685623689132	7.999193681276927E-5	0.0	0.0	7.999193681276927E-5
20	1.27963101319387	7.999193681276927E-5	0.0	0.0	7.999193681276927E-5
21	1.4974490571350407	7.999193681276927E-5	0.0	0.0	7.999193681276927E-5
22	1.7831802554302527	7.999193681276927E-5	0.0	0.0	7.999193681276927E-5
23	2.173220939329316	7.999193681276927E-5	0.0	0.0	1.5998387362553855E-4
24	2.6815696977744645	7.999193681276927E-5	0.0	0.0	1.5998387362553855E-4
25	3.2916681998454558	7.999193681276927E-5	0.0	0.0	1.5998387362553855E-4
26	4.03127364761632	7.999193681276927E-5	0.0	0.0	1.5998387362553855E-4
27	4.905745500853514	7.999193681276927E-5	0.0	0.0	1.5998387362553855E-4
28	5.887006589735755	7.999193681276927E-5	0.0	0.0	1.5998387362553855E-4
29	6.98337607569157	7.999193681276927E-5	0.0	0.0	1.5998387362553855E-4
30	8.185494902113867	7.999193681276927E-5	0.0	0.0	1.5998387362553855E-4
31	9.486963714057623	7.999193681276927E-5	0.0	0.0	1.5998387362553855E-4
32	10.862745035300442	7.999193681276927E-5	0.0	0.0	1.5998387362553855E-4
33	12.333956737160895	7.999193681276927E-5	0.0	0.0	1.5998387362553855E-4
34	13.861402770600723	7.999193681276927E-5	0.0	0.0	2.3997581043830782E-4
35	15.461881442350611	7.999193681276927E-5	0.0	0.0	2.3997581043830782E-4
36	17.120834219910634	7.999193681276927E-5	0.0	0.0	2.3997581043830782E-4
37	18.848260095382386	7.999193681276927E-5	0.0	0.0	2.3997581043830782E-4
38	20.634480044411525	7.999193681276927E-5	0.0	0.0	2.3997581043830782E-4
39	22.45997603441573	7.999193681276927E-5	0.0	0.0	2.3997581043830782E-4
40	24.348345686754776	7.999193681276927E-5	0.0	0.0	2.3997581043830782E-4
41	26.271831799354626	7.999193681276927E-5	0.0	0.0	2.3997581043830782E-4
42	28.267790606706843	7.999193681276927E-5	0.0	0.0	2.3997581043830782E-4
43	30.225913227946624	7.999193681276927E-5	0.0	0.0	2.3997581043830782E-4
44	32.25322887452945	7.999193681276927E-5	0.0	0.0	2.3997581043830782E-4
45	34.29790277140064	7.999193681276927E-5	0.0	0.0	2.3997581043830782E-4
46	36.33537739395869	7.999193681276927E-5	0.0	0.0	2.3997581043830782E-4
47	38.38285100861833	7.999193681276927E-5	0.0	0.0	2.3997581043830782E-4
48	40.46032159958276	7.999193681276927E-5	0.0	0.0	2.3997581043830782E-4
49	42.47387863303379	7.999193681276927E-5	0.0	0.0	2.3997581043830782E-4
50	44.53111126398459	7.999193681276927E-5	0.0	0.0	2.3997581043830782E-4
>>END_MODULE