##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-5A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 731038 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.280420443260134 40.0 40.0 40.0 40.0 40.0 2 39.17360793830143 40.0 40.0 40.0 40.0 40.0 3 39.13545397092901 40.0 40.0 40.0 40.0 40.0 4 39.0529138020185 40.0 40.0 40.0 40.0 40.0 5 39.198219518000435 40.0 40.0 40.0 40.0 40.0 6 39.25713847980543 40.0 40.0 40.0 40.0 40.0 7 39.29651810165819 40.0 40.0 40.0 40.0 40.0 8 39.30960634057327 40.0 40.0 40.0 40.0 40.0 9 39.33044793841086 40.0 40.0 40.0 40.0 40.0 10-11 39.320273364722496 40.0 40.0 40.0 40.0 40.0 12-13 39.322120053950684 40.0 40.0 40.0 40.0 40.0 14-15 39.32735644385107 40.0 40.0 40.0 40.0 40.0 16-17 39.46426861531138 40.0 40.0 40.0 40.0 40.0 18-19 39.48062344228344 40.0 40.0 40.0 40.0 40.0 20-21 39.490609243295154 40.0 40.0 40.0 40.0 40.0 22-23 39.49248602671817 40.0 40.0 40.0 40.0 40.0 24-25 39.49548176702169 40.0 40.0 40.0 40.0 40.0 26-27 39.48673529966979 40.0 40.0 40.0 40.0 40.0 28-29 39.48280663932655 40.0 40.0 40.0 40.0 40.0 30-31 39.47386009276157 40.0 40.0 40.0 40.0 40.0 32-33 39.46970932679335 40.0 40.0 40.0 40.0 40.0 34-35 39.46871129769732 40.0 40.0 40.0 40.0 40.0 36-37 39.46201780101377 40.0 40.0 40.0 40.0 40.0 38-39 39.450863376385925 40.0 40.0 40.0 40.0 40.0 40-41 39.44174659983817 40.0 40.0 40.0 40.0 40.0 42-43 39.43234320567463 40.0 40.0 40.0 40.0 40.0 44-45 39.4132151502027 40.0 40.0 40.0 40.0 40.0 46-47 39.41044600427051 40.0 40.0 40.0 40.0 40.0 48-49 39.411461909650775 40.0 40.0 40.0 40.0 40.0 50-51 39.40966433466864 40.0 40.0 40.0 40.0 40.0 52-53 39.394315856675064 40.0 40.0 40.0 40.0 40.0 54-55 39.37370004714239 40.0 40.0 40.0 40.0 40.0 56-57 39.37073176150745 40.0 40.0 40.0 40.0 40.0 58-59 39.36391483261323 40.0 40.0 40.0 40.0 40.0 60-61 39.32833340593169 40.0 40.0 40.0 40.0 40.0 62-63 39.366043275252174 40.0 40.0 40.0 40.0 40.0 64-65 39.36046374431825 40.0 40.0 40.0 40.0 40.0 66-67 39.36098200150611 40.0 40.0 40.0 40.0 40.0 68-69 39.34974491319298 40.0 40.0 40.0 40.0 40.0 70-71 39.35448583480019 40.0 40.0 40.0 40.0 40.0 72-73 39.34500710526257 40.0 40.0 40.0 40.0 40.0 74-75 39.34230077498948 40.0 40.0 40.0 40.0 40.0 76-77 39.330256210928646 40.0 40.0 40.0 40.0 40.0 78-79 39.31892861006918 40.0 40.0 40.0 40.0 40.0 80-81 39.3096276994086 40.0 40.0 40.0 40.0 40.0 82-83 39.333627091349314 40.0 40.0 40.0 40.0 40.0 84-85 39.350130738666856 40.0 40.0 40.0 40.0 40.0 86-87 39.34069791419505 40.0 40.0 40.0 40.0 40.0 88-89 39.37997485674519 40.0 40.0 40.0 40.0 40.0 90-91 39.482314312666176 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23254945325894738 1101 2 0.20628171369307324 1101 3 0.252156052889994 1101 4 0.21668203345227965 1101 5 0.10868779399218198 1101 6 -0.01775116491571538 1101 7 0.12426622316498737 1101 8 -0.034977822471645936 1101 9 0.12286942081293972 1101 10-11 -0.07965464790702015 1101 12-13 -0.028309731106865854 1101 14-15 0.031618730062305644 1101 16-17 -0.062753261522289 1101 18-19 -0.04074398413356306 1101 20-21 -0.06956232995612766 1101 22-23 -0.04496649016296317 1101 24-25 -0.011623170625654211 1101 26-27 0.058787943603768156 1101 28-29 0.0035212936610093948 1101 30-31 0.029426680555417306 1101 32-33 -0.049614065416200503 1101 34-35 -0.029072403409323044 1101 36-37 0.023756817744221337 1101 38-39 0.0016514041860773432 1101 40-41 0.10059721423715473 1101 42-43 0.08946206009481017 1101 44-45 0.0030315485427792055 1101 46-47 0.039700810479203597 1101 48-49 0.10118894837224701 1101 50-51 0.0456332091058016 1101 52-53 -0.015960555590076808 1101 54-55 0.16199506180721812 1101 56-57 0.10919784148071443 1101 58-59 0.19332268203430658 1101 60-61 0.12476705801965693 1101 62-63 0.11701742418835437 1101 64-65 0.1527688022176008 1101 66-67 0.13739267266911526 1101 68-69 0.2125567463660616 1101 70-71 -0.08970237914815726 1101 72-73 0.1372627443294121 1101 74-75 0.051114962810245856 1101 76-77 0.11573267088356687 1101 78-79 0.009238469355011603 1101 80-81 0.2954591734688634 1101 82-83 0.5766628415843869 1101 84-85 -0.15510414750151114 1101 86-87 -0.37745120297586254 1101 88-89 -0.9365146964522282 1101 90-91 -0.9843306986999778 1102 1 0.2613914864898632 1102 2 0.17678265546679484 1102 3 0.164951330494425 1102 4 -0.04445016384164546 1102 5 0.04270111409407917 1102 6 0.07778117878793012 1102 7 0.15909536824815262 1102 8 0.017554622400204778 1102 9 0.10225384068146326 1102 10-11 -2.8447387087737752E-5 1102 12-13 -0.1046337558282886 1102 14-15 -0.07480681525139232 1102 16-17 0.0900198272795194 1102 18-19 0.06358544683504874 1102 20-21 0.04221489992191607 1102 22-23 0.05002932101850632 1102 24-25 0.03189279974229464 1102 26-27 0.03562156054909593 1102 28-29 0.022726202478892787 1102 30-31 -0.030674055699066116 1102 32-33 0.034911689888105 1102 34-35 -0.057776641287098585 1102 36-37 0.026457841454735842 1102 38-39 -0.02915004928581766 1102 40-41 -1.5978317257037133E-4 1102 42-43 0.06275265315218803 1102 44-45 0.08573494075455557 1102 46-47 -0.04550137757706807 1102 48-49 -0.014673532692398794 1102 50-51 0.030253074747932374 1102 52-53 0.018860909537892212 1102 54-55 0.12748567358901397 1102 56-57 0.006356664803753631 1102 58-59 0.11993675791495662 1102 60-61 0.024207867950323703 1102 62-63 -0.07170165900847536 1102 64-65 -0.19412792893853492 1102 66-67 0.24218756209022985 1102 68-69 0.009606266009285491 1102 70-71 0.17324051491083026 1102 72-73 0.18692772328350316 1102 74-75 0.18262730566472385 1102 76-77 0.13050988206937575 1102 78-79 0.007081882088940006 1102 80-81 -0.13792884483236634 1102 82-83 0.1670199291860186 1102 84-85 -0.3078991785574132 1102 86-87 0.21392170982561964 1102 88-89 0.2775615498820869 1102 90-91 0.037891523522247894 1103 1 0.2562172559023068 1103 2 0.0833776193125857 1103 3 0.03499965722673437 1103 4 -0.051324914032406355 1103 5 0.03318111118721134 1103 6 -0.14110621629219366 1103 7 -0.08030000255958925 1103 8 0.01506924759550543 1103 9 0.00484458956413647 1103 10-11 0.026097056773373595 1103 12-13 -0.058051146184737945 1103 14-15 0.02617062016156524 1103 16-17 0.0063673437215143736 1103 18-19 6.304575950366598E-5 1103 20-21 -0.07251930305035614 1103 22-23 -0.024212167299502596 1103 24-25 0.01698055091412698 1103 26-27 0.00685807085032053 1103 28-29 -0.08357807425523589 1103 30-31 -0.06105085886756001 1103 32-33 0.010960558598142711 1103 34-35 -0.009507231327795296 1103 36-37 -0.044505739909091346 1103 38-39 -0.06729538092992726 1103 40-41 0.013335753484319923 1103 42-43 0.05365441102797064 1103 44-45 0.049776839767005754 1103 46-47 0.03564771698087554 1103 48-49 -0.014111053479219038 1103 50-51 0.06348775246289051 1103 52-53 -0.0693397806894609 1103 54-55 0.06071291298664505 1103 56-57 0.031210175677927054 1103 58-59 -0.02860498964628988 1103 60-61 0.03771032286728371 1103 62-63 -0.18918840734328768 1103 64-65 -0.13177862332650392 1103 66-67 0.08010006875213804 1103 68-69 -0.22622770007851756 1103 70-71 0.17857040919218292 1103 72-73 0.19131227974607157 1103 74-75 0.2563608979746448 1103 76-77 -0.24565980961981637 1103 78-79 0.06476607380944444 1103 80-81 -0.10699724457196425 1103 82-83 0.11868612408972012 1103 84-85 0.2660845612799818 1103 86-87 -0.253962622590727 1103 88-89 0.5271159170835134 1103 90-91 0.5712248568555793 1104 1 0.316462613992492 1104 2 0.06826930808358611 1104 3 0.2165978004628073 1104 4 0.1565552804857191 1104 5 0.25563358687244175 1104 6 0.16244705514900915 1104 7 0.09049464819195663 1104 8 -0.003957056560103922 1104 9 -0.1410329524340952 1104 10-11 0.15039524421811024 1104 12-13 0.11059938431923655 1104 14-15 0.06287210115183939 1104 16-17 0.016905584216644343 1104 18-19 -0.0938554997754153 1104 20-21 -0.11683848961092735 1104 22-23 -0.018688847228773398 1104 24-25 0.04665285418556664 1104 26-27 -0.03181345876948427 1104 28-29 0.006477739184198583 1104 30-31 0.028850224244735045 1104 32-33 0.007119828266397121 1104 34-35 0.030880078903706476 1104 36-37 0.027669127945969763 1104 38-39 -0.045285315301669016 1104 40-41 -0.05772529154167927 1104 42-43 -0.021504821183555123 1104 44-45 -0.04177542075112228 1104 46-47 0.021484235556471276 1104 48-49 0.038349538668818184 1104 50-51 0.03241061361000419 1104 52-53 0.003940681863845441 1104 54-55 0.048245963815233495 1104 56-57 0.035093917858944224 1104 58-59 -0.05523326763184855 1104 60-61 -0.08250815896612096 1104 62-63 -0.09918785801946939 1104 64-65 0.08177636253721232 1104 66-67 -0.038529576848880254 1104 68-69 0.1201589632174489 1104 70-71 0.25862287419362673 1104 72-73 0.1595664860755619 1104 74-75 -0.03167565440094222 1104 76-77 0.2774712120283169 1104 78-79 0.2115231776698394 1104 80-81 0.5285308636834145 1104 82-83 -0.06594884226440456 1104 84-85 0.6730532023949678 1104 86-87 0.5437176281929723 1104 88-89 0.2138386061591433 1104 90-91 0.5712248568555793 1201 1 0.3221351510011701 1201 2 0.21142270267265673 1201 3 0.2966705416572992 1201 4 0.2152778430176241 1201 5 0.07468986558607327 1201 6 0.17393569731338232 1201 7 0.004969507814209351 1201 8 0.10369672087735893 1201 9 0.03269726706565024 1201 10-11 0.08627770976175952 1201 12-13 0.06168731183328191 1201 14-15 0.0014137678185051072 1201 16-17 -0.02704222078590135 1201 18-19 0.04976847929553685 1201 20-21 -0.024431649386322363 1201 22-23 0.0030138709948204223 1201 24-25 0.10778257598239804 1201 26-27 0.09441803689156814 1201 28-29 0.023723464043818865 1201 30-31 0.017344034862396995 1201 32-33 -0.01643614702787488 1201 34-35 0.05486282695031264 1201 36-37 -0.04461146981186204 1201 38-39 -0.0022108406722480822 1201 40-41 -0.10113539271436878 1201 42-43 -0.02273618396584709 1201 44-45 -0.065601499956351 1201 46-47 0.045632778910913885 1201 48-49 -0.06147111481234191 1201 50-51 0.024308967774878454 1201 52-53 -0.08273089773771147 1201 54-55 0.022315980492038534 1201 56-57 -0.06037322550820079 1201 58-59 -0.1619619128595815 1201 60-61 0.04024987661267687 1201 62-63 -0.2574641481559752 1201 64-65 -0.2544652704570751 1201 66-67 -0.17582756070525818 1201 68-69 -0.3322338540863683 1201 70-71 -0.200749369896009 1201 72-73 -0.5479316278170216 1201 74-75 -0.6149334512180644 1201 76-77 -0.17622196580279592 1201 78-79 -0.39171688564309903 1201 80-81 -0.3616435253904555 1201 82-83 -0.11746502321447139 1201 84-85 -0.6499575502932089 1201 86-87 -0.1808977564224179 1201 88-89 0.47669574901628664 1201 90-91 0.5712248568555793 1202 1 -0.04180009787191352 1202 2 0.03215605692795975 1202 3 -0.10121807010730066 1202 4 -0.011145027680690589 1202 5 -0.10034676751892846 1202 6 0.09032648504115315 1202 7 0.0211218952386929 1202 8 -0.07451753421803886 1202 9 0.0266031543969234 1202 10-11 0.0025363587532893916 1202 12-13 -0.0828488694044367 1202 14-15 -0.05611062868894123 1202 16-17 -0.05819392785320332 1202 18-19 0.004846028216363152 1202 20-21 -0.00829971200435864 1202 22-23 -0.02885673372080788 1202 24-25 -0.010722274217108918 1202 26-27 -0.003699694003181264 1202 28-29 -0.022440147307840164 1202 30-31 0.09534836240601408 1202 32-33 -0.011304530716216732 1202 34-35 -0.11350291100796284 1202 36-37 0.004812717588528415 1202 38-39 -0.06892551880045517 1202 40-41 -0.0697301936983763 1202 42-43 -0.03935944202282826 1202 44-45 0.03578973888419057 1202 46-47 0.016379104488628116 1202 48-49 -0.09050232156575788 1202 50-51 0.07502732142106794 1202 52-53 -0.07608671265263212 1202 54-55 -0.13330118633619037 1202 56-57 -0.09703882273060316 1202 58-59 0.022732394196289363 1202 60-61 -0.04869059026951561 1202 62-63 -0.055900185239345035 1202 64-65 -0.1342971304402809 1202 66-67 -0.18342214672932045 1202 68-69 -0.37714179141758564 1202 70-71 -0.27988398241667056 1202 72-73 -0.53424273405642 1202 74-75 -0.3548078810182602 1202 76-77 -0.11606645497226964 1202 78-79 -0.2473122678934203 1202 80-81 -0.27712881637571485 1202 82-83 -0.3761488509396287 1202 84-85 -0.08789917855741436 1202 86-87 0.4014577411873219 1202 88-89 -0.4524047800842439 1202 90-91 -0.2708804063023109 1203 1 0.20139851142839404 1203 2 0.27025648757076226 1203 3 0.10743113379614044 1203 4 0.23649117792161434 1203 5 -0.0435651310762708 1203 6 0.13440218335414045 1203 7 0.07962926357657096 1203 8 4.98071645019138E-4 1203 9 0.10556961166847145 1203 10-11 0.12972216729503572 1203 12-13 0.02909297406282718 1203 14-15 0.011670178074922433 1203 16-17 0.013476097037163015 1203 18-19 0.03330796176304318 1203 20-21 0.045853818081383224 1203 22-23 -0.04033948825441058 1203 24-25 -0.0037798381221278987 1203 26-27 -0.016236535692563336 1203 28-29 -0.07187161979015855 1203 30-31 0.01191112168063313 1203 32-33 0.08089478038390041 1203 34-35 -0.002022482312391105 1203 36-37 -0.0032891570126381 1203 38-39 0.047961656015672816 1203 40-41 -0.03835362673651588 1203 42-43 -0.002136205340704578 1203 44-45 0.023381487028551362 1203 46-47 -0.04225016368317824 1203 48-49 -0.04986543457830095 1203 50-51 0.06856101253615066 1203 52-53 -0.012645100878884818 1203 54-55 -0.08161449130827236 1203 56-57 0.08911958895064487 1203 58-59 0.1488842191773614 1203 60-61 -0.019368824373529492 1203 62-63 0.01698531225868294 1203 64-65 0.023393767021360645 1203 66-67 0.04743433533128183 1203 68-69 -0.03853994934016214 1203 70-71 0.10379479813914116 1203 72-73 -0.16040302264039497 1203 74-75 -0.05288679089861148 1203 76-77 0.08035949762199834 1203 78-79 0.10945269291778459 1203 80-81 0.04008002790143905 1203 82-83 -0.6816846702122845 1203 84-85 0.030196059537829 1203 86-87 -0.0031451169050669137 1203 88-89 0.4865479657650553 1203 90-91 0.5712248568555793 1204 1 0.2802254879503181 1204 2 0.10297790707001298 1204 3 0.09882560821170472 1204 4 -0.08733674174740003 1204 5 -0.1437298674352263 1204 6 -0.14914052863389315 1204 7 -0.14362849223635976 1204 8 0.008823922678082852 1204 9 -0.07302371588023249 1204 10-11 -0.1623918654761809 1204 12-13 -0.14066042441052673 1204 14-15 -0.24903579896259487 1204 16-17 -0.01510050536974461 1204 18-19 -0.006912860409713062 1204 20-21 -0.1500124310855071 1204 22-23 -0.052725650400262225 1204 24-25 -0.17199854764540135 1204 26-27 -0.1107962117529766 1204 28-29 -0.1051614426042562 1204 30-31 -0.13526157483978096 1204 32-33 -0.11808958523775459 1204 34-35 -0.024494091061384893 1204 36-37 -0.1702922975076504 1204 38-39 -0.11526684865320647 1204 40-41 -0.16874175219818 1204 42-43 -0.15428091882674977 1204 44-45 -0.1699478156064771 1204 46-47 -0.19178158071493812 1204 48-49 0.009233778309798879 1204 50-51 -0.06238052360164659 1204 52-53 -0.008335575877183032 1204 54-55 -0.1012059598842967 1204 56-57 0.02333132397760096 1204 58-59 -0.20441953012132785 1204 60-61 -0.2722458593271284 1204 62-63 -0.04709870863209886 1204 64-65 -0.10326182594612021 1204 66-67 -0.07296958016006272 1204 68-69 0.006442764431831449 1204 70-71 -0.09153157093248865 1204 72-73 -0.14657725815016676 1204 74-75 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2401 52-53 0.23195706126964666 2401 54-55 0.20828627575326664 2401 56-57 0.09429905881729184 2401 58-59 0.2162035216304119 2401 60-61 0.08307601622608018 2401 62-63 0.14885493937656946 2401 64-65 0.15644574445702375 2401 66-67 0.13659272025919478 2401 68-69 0.29595905325189165 2401 70-71 0.30098686706697464 2401 72-73 0.10673132528325624 2401 74-75 0.27857837907133387 2401 76-77 0.17494476083480492 2401 78-79 0.10334520158212257 2401 80-81 0.2819690422712924 2401 82-83 0.07381643986580855 2401 84-85 -0.2714030197801449 2401 86-87 0.47836551551691286 2401 88-89 -0.09473282241228276 2401 90-91 0.5712248568555793 2402 1 0.4198962837834088 2402 2 0.3374503943609213 2402 3 0.35229534912899396 2402 4 0.3741622259315278 2402 5 0.2424681819217085 2402 6 0.13534870138789756 2402 7 0.22442729659378102 2402 8 0.2068952025122357 2402 9 0.030599467657225432 2402 10-11 0.1787192648862188 2402 12-13 0.14798673111087624 2402 14-15 0.17925563275482403 2402 16-17 0.043544312887640046 2402 18-19 0.07179353290902668 2402 20-21 0.09368181800743258 2402 22-23 0.07260155772196697 2402 24-25 0.037549723928421486 2402 26-27 0.053313313640991566 2402 28-29 0.0804601052019791 2402 30-31 0.1262224004043162 2402 32-33 0.12517549747462908 2402 34-35 0.13647548997764147 2402 36-37 0.05696160270387196 2402 38-39 0.19616673760891956 2402 40-41 0.10609231890282445 2402 42-43 0.11256534534575735 2402 44-45 0.07872812483154235 2402 46-47 0.09511494376830854 2402 48-49 0.1619992418818086 2402 50-51 0.07562450059110404 2402 52-53 0.13356698166326453 2402 54-55 0.17298276003182878 2402 56-57 0.0725905931159403 2402 58-59 0.21718014589528423 2402 60-61 0.13629678869776285 2402 62-63 0.3206229557127287 2402 64-65 0.2160064702270077 2402 66-67 0.2446099836817197 2402 68-69 0.3695080285137351 2402 70-71 0.1207282545210191 2402 72-73 0.3446942221865825 2402 74-75 0.20984514342354998 2402 76-77 0.42792275500644905 2402 78-79 0.2805725456143975 2402 80-81 0.43299154266923523 2402 82-83 0.24976803828213434 2402 84-85 0.33629818477584905 2402 86-87 -0.2055085622832209 2402 88-89 0.7624100347305713 2402 90-91 0.5712248568555793 2403 1 -0.331381905821857 2403 2 -0.06511311486060123 2403 3 -0.03558445587421488 2403 4 -0.012980897069915898 2403 5 -0.006278081579296213 2403 6 0.03406805360194198 2403 7 0.12921668198940495 2403 8 0.03728639815845014 2403 9 0.006948423265058068 2403 10-11 0.06424372095261077 2403 12-13 0.11240900588296654 2403 14-15 0.046873722596565415 2403 16-17 0.013220144492741781 2403 18-19 0.08382471859897578 2403 20-21 0.14639190429601 2403 22-23 0.026810156103373117 2403 24-25 0.06059021286698396 2403 26-27 0.05061394810901021 2403 28-29 0.06892827768235321 2403 30-31 0.037983495100384346 2403 32-33 0.0698508426921336 2403 34-35 0.05907839800558179 2403 36-37 -0.008710343915701912 2403 38-39 0.10840416994125235 2403 40-41 0.07329966547708722 2403 42-43 0.0550783004075015 2403 44-45 0.05939565523713952 2403 46-47 0.025357116622352294 2403 48-49 0.09625670342153825 2403 50-51 0.07729809741358906 2403 52-53 0.1654884909766281 2403 54-55 0.010827640942991934 2403 56-57 0.11375565098539653 2403 58-59 0.021933416316414878 2403 60-61 0.06084416768329959 2403 62-63 0.24531327724748309 2403 64-65 0.08483332598304827 2403 66-67 0.21668928310550228 2403 68-69 0.198791655776958 2403 70-71 0.021903635430724933 2403 72-73 -0.05387734299175406 2403 74-75 0.03639906856307107 2403 76-77 0.09288536364935851 2403 78-79 0.1812481676433535 2403 80-81 0.2504869010768971 2403 82-83 0.16792280968646622 2403 84-85 -0.11855099550608372 2403 86-87 -0.010489115864523058 2403 88-89 -0.03892181876554446 2403 90-91 0.5712248568555793 2404 1 -0.42236060407384457 2404 2 -0.2611384783196158 2404 3 -0.4782945778011296 2404 4 -0.4181124447554012 2404 5 -0.16193727880622077 2404 6 -0.22306664750918515 2404 7 -0.11940155270195163 2404 8 0.02789868326581768 2404 9 0.027583255516972827 2404 10-11 0.02828721081420582 2404 12-13 0.09783915143285782 2404 14-15 0.05128438649646938 2404 16-17 0.0410837187732227 2404 18-19 0.0040442591048446275 2404 20-21 -0.029864836352871293 2404 22-23 -0.0628659526157378 2404 24-25 -0.02319174120376033 2404 26-27 2.5840667394305683E-4 2404 28-29 -0.016223536986117892 2404 30-31 0.03592429505160055 2404 32-33 -0.004426509118516719 2404 34-35 0.049100558893329094 2404 36-37 -0.019182846880887894 2404 38-39 0.05401509689910711 2404 40-41 0.016769669232665763 2404 42-43 0.07059724553928959 2404 44-45 0.07600547670168112 2404 46-47 0.0681670337408633 2404 48-49 0.10240209953692414 2404 50-51 0.051797908689216854 2404 52-53 0.046726061893636484 2404 54-55 0.023689382869491737 2404 56-57 0.05290604597211512 2404 58-59 0.05698439095387897 2404 60-61 0.17351859415529702 2404 62-63 0.15606941897800652 2404 64-65 0.15567142185182803 2404 66-67 0.11288928055319758 2404 68-69 0.01898054009237171 2404 70-71 0.09762266961180188 2404 72-73 0.03402357420738156 2404 74-75 0.32578557270991837 2404 76-77 0.25402946656715386 2404 78-79 0.2209738294389254 2404 80-81 0.27019753035008165 2404 82-83 0.020665289564810507 2404 84-85 -0.060941600345401525 2404 86-87 -0.30793608989804255 2404 88-89 -0.4838527504779293 2404 90-91 -0.6560478704171473 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 23.0 31 401.0 32 2767.0 33 5255.0 34 6898.0 35 8746.0 36 12561.0 37 19543.0 38 53287.0 39 311891.0 40 309666.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.784556626796093 13.85561781153219 26.974879348646848 31.38494621302487 2 27.807220345011558 17.947030739134597 26.831694004021944 27.414054911831897 3 26.59411190177201 19.572687453661526 20.264036966816864 33.5691636777496 4 26.287689559229477 21.394920647079907 19.318010828438467 32.99937896525215 5 26.897097004533283 21.647848675445054 19.399675529863018 32.05537879015865 6 27.032384089472778 24.525811243738353 22.450816510222452 25.99098815656642 7 25.34081675644768 24.357283752691377 22.723032181637617 27.578867309223327 8 24.361934673710532 25.722602655402316 23.46266541547772 26.452797255409433 9 24.21761933032209 25.720003611303376 23.893149193338786 26.169227865035744 10-11 24.12911503916349 24.85178609046315 23.760734736087592 27.258364134285767 12-13 24.26782191897001 24.592428847747996 23.604860486048604 27.53488874723339 14-15 24.510285375042063 24.01530426598891 23.59138649427253 27.883023864696497 16-17 24.78687838388702 24.053947947986288 23.182721007663076 27.976452660463615 18-19 24.726758390124726 24.22363816928805 23.155909815905602 27.893693624681614 20-21 24.862592642242948 24.35872006653553 23.01843406225121 27.76025322897031 22-23 24.628063110262392 23.977618126554297 23.274371510099336 28.11994725308397 24-25 24.615888640535786 24.197716124196006 23.387361532505835 27.799033702762372 26-27 24.979686418489873 24.285946831765244 23.126773163638553 27.607593586106333 28-29 24.92202867703184 23.99711095729634 23.188261075347658 27.89259929032417 30-31 25.080724313048236 24.067164817126343 23.27042046406648 27.58169040575894 32-33 25.160864092045504 24.089138891794722 23.186053320171673 27.5639436959881 34-35 25.096606559974184 23.808251401187533 23.304502854940132 27.790639183898154 36-37 25.133424228390233 24.005832136107593 23.274831402894247 27.585912232607928 38-39 25.332109056762203 24.04894221863272 23.124324965306823 27.494623759298253 40-41 25.304729977848588 23.816913254125254 23.23106003557087 27.647296732455285 42-43 25.335415912136266 23.902354853813573 23.223389828375975 27.53883940567419 44-45 25.48366332217009 23.91134381994016 23.117797332908676 27.48719552498108 46-47 25.41961339962024 23.806135520197046 23.1080841739331 27.66616690624961 48-49 25.5405839696906 23.99453327684238 23.13449184272829 27.33039091073873 50-51 25.72153355111374 23.940495915711008 22.938609371808678 27.399361161366574 52-53 25.55850607057456 23.744599896952163 23.090774576248794 27.606119456224487 54-55 25.736491068589114 23.948466318248947 22.974222994117365 27.340819619044577 56-57 25.761638960972228 23.92972302869916 22.95595565376018 27.35268235656843 58-59 25.846945921225633 23.589435774309724 22.93989024181101 27.623728062653637 60-61 25.867574069485848 23.92842014402289 22.85963762498102 27.34436816151024 62-63 25.954112585285067 23.759428612576283 22.67255036094259 27.61390844119606 64-65 26.020053557922278 23.46327602771929 22.77540423966148 27.74126617469695 66-67 26.13198367079767 23.64490705972081 22.642011168613333 27.581098100868186 68-69 26.27379689945968 23.737144203740172 22.507027554747598 27.482031342052547 70-71 26.307567013054133 23.4238326063634 22.50056774132922 27.768032639253242 72-73 26.28760848046776 23.652770424253585 22.40279919891347 27.656821896365187 74-75 26.56713146864641 23.52464181026217 22.11763416705476 27.790592554036653 76-77 26.450923059660607 23.209488670490472 22.300619773343804 28.038968496505113 78-79 26.652415769912796 23.414385308620982 21.894559558222202 28.038639363244023 80-81 27.08582915389145 23.172044102418322 21.545615139393693 28.196511604296532 82-83 27.1601344377696 22.580517109058672 21.457114880779105 28.802233572392623 84-85 27.96932792061344 22.377988272440234 20.636896707262064 29.01578709968426 86-87 28.798317118064688 21.038653694451746 20.29450433867999 29.868524848803574 88-89 28.20569704499068 20.898038867690126 20.174815866536516 30.721448220782676 90-91 24.933949801849405 22.611184500220165 22.699251431087628 29.7556142668428 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.5 14 7.0 15 10.5 16 11.5 17 18.0 18 33.0 19 48.0 20 66.0 21 94.0 22 128.5 23 186.5 24 281.0 25 379.0 26 495.0 27 684.5 28 968.5 29 1329.0 30 1744.0 31 2292.5 32 3037.5 33 3897.5 34 5101.0 35 6660.0 36 8496.0 37 10605.5 38 13162.0 39 16374.0 40 19907.0 41 24079.0 42 28650.0 43 33098.5 44 37696.0 45 42443.0 46 47318.0 47 52123.5 48 56256.0 49 59839.0 50 62859.5 51 64595.0 52 65265.5 53 65500.5 54 64603.5 55 62576.5 56 60043.0 57 56973.0 58 53509.0 59 49512.0 60 45272.5 61 41347.0 62 38137.0 63 34989.5 64 31313.0 65 27742.0 66 24337.5 67 21326.5 68 18618.0 69 15786.0 70 13207.5 71 10993.5 72 8968.5 73 7084.0 74 5455.5 75 4249.0 76 3246.0 77 2339.0 78 1730.5 79 1262.5 80 858.0 81 573.5 82 393.0 83 263.0 84 162.5 85 101.0 86 61.0 87 35.5 88 24.5 89 15.0 90 9.0 91 7.0 92 8.0 93 9.5 94 7.0 95 2.5 96 0.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 8.207507680859271E-4 2 0.006566006144687417 3 5.471671787239514E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 12352.0 32-33 26688.0 34-35 29008.0 36-37 30895.0 38-39 32678.0 40-41 34480.0 42-43 34850.0 44-45 35584.0 46-47 36040.0 48-49 35794.0 50-51 35485.0 52-53 34520.0 54-55 33159.0 56-57 32014.0 58-59 29956.0 60-61 28518.0 62-63 26982.0 64-65 25058.0 66-67 23060.0 68-69 21241.0 70-71 19490.0 72-73 17832.0 74-75 15884.0 76-77 14462.0 78-79 12740.0 80-81 11764.0 82-83 10447.0 84-85 8972.0 86-87 7992.0 88-89 7002.0 90-91 6091.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.87879703200643 #Duplication Level Percentage of deduplicated Percentage of total 1 99.88210422063575 99.7610441458259 2 0.1144415108752307 0.22860560873486646 3 0.0034542684890058305 0.010350245439224066 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0013679179468098785 0.0 0.0 0.0 1.3679179468098786E-4 2 0.0013679179468098785 0.0 0.0 0.0 1.3679179468098786E-4 3 0.0013679179468098785 0.0 0.0 0.0 1.3679179468098786E-4 4 0.0013679179468098785 0.0 0.0 0.0 2.735835893619757E-4 5 0.0013679179468098785 0.0 0.0 0.0 2.735835893619757E-4 6 0.0015047097414908664 0.0 0.0 0.0 2.735835893619757E-4 7 0.0016415015361718542 0.0 0.0 0.0 4.1037538404296354E-4 8 0.006155630760644453 0.0 0.0 0.0 4.1037538404296354E-4 9 0.006429214350006429 0.0 0.0 0.0 4.1037538404296354E-4 10-11 0.006566006144687417 0.0 0.0 0.0 4.7877128138345746E-4 12-13 0.006566006144687417 0.0 0.0 0.0 5.471671787239514E-4 14-15 0.006566006144687417 0.0 0.0 0.0 5.471671787239514E-4 16-17 0.006566006144687417 0.0 0.0 0.0 5.471671787239514E-4 18-19 0.006566006144687417 0.0 0.0 0.0 5.471671787239514E-4 20-21 0.006566006144687417 0.0 0.0 0.0 5.471671787239514E-4 22-23 0.006976381528730381 0.0 0.0 0.0 5.471671787239514E-4 24-25 0.007113173323411368 0.0 0.0 0.0 5.471671787239514E-4 26-27 0.007249965118092356 0.0 0.0 0.0 5.471671787239514E-4 28-29 0.0074551528101138385 0.0 1.3679179468098786E-4 0.0 6.155630760644453E-4 30-31 0.007865528194156802 0.0 1.3679179468098786E-4 0.0 6.839589734049393E-4 32-33 0.008481091270221248 0.0 1.3679179468098786E-4 0.0 6.839589734049393E-4 34-35 0.008754674859583223 0.0 1.3679179468098786E-4 0.0 6.839589734049393E-4 36-37 0.009096654346285692 6.839589734049393E-5 1.3679179468098786E-4 0.0 6.839589734049393E-4 38-39 0.009438633832988161 1.3679179468098786E-4 1.3679179468098786E-4 0.0 6.839589734049393E-4 40-41 0.009438633832988161 1.3679179468098786E-4 1.3679179468098786E-4 0.0 6.839589734049393E-4 42-43 0.00957542562766915 1.3679179468098786E-4 1.3679179468098786E-4 0.0 6.839589734049393E-4 44-45 0.009849009217031125 1.3679179468098786E-4 1.3679179468098786E-4 0.0 8.207507680859271E-4 46-47 0.010327780498414582 1.3679179468098786E-4 1.3679179468098786E-4 0.0 8.207507680859271E-4 48-49 0.010396176395755076 1.3679179468098786E-4 1.3679179468098786E-4 0.0 8.207507680859271E-4 50-51 0.01046457229309557 1.3679179468098786E-4 1.3679179468098786E-4 0.0 8.207507680859271E-4 52-53 0.010943343574479028 1.3679179468098786E-4 1.3679179468098786E-4 0.0 8.207507680859271E-4 54-55 0.01114853126650051 1.3679179468098786E-4 1.3679179468098786E-4 0.0 8.207507680859271E-4 56-57 0.011353718958521991 1.3679179468098786E-4 1.3679179468098786E-4 0.0 8.207507680859271E-4 58-59 0.011695698445224462 1.3679179468098786E-4 1.3679179468098786E-4 0.0 8.89146665426421E-4 60-61 0.011900886137245943 1.3679179468098786E-4 1.3679179468098786E-4 0.0 9.57542562766915E-4 62-63 0.011969282034586437 1.3679179468098786E-4 1.3679179468098786E-4 0.0 9.57542562766915E-4 64-65 0.012037677931926932 1.3679179468098786E-4 1.3679179468098786E-4 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