##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-4C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 721185 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.28811054029133 40.0 40.0 40.0 40.0 40.0 2 39.174178608817435 40.0 40.0 40.0 40.0 40.0 3 39.15358195192634 40.0 40.0 40.0 40.0 40.0 4 39.06701331835798 40.0 40.0 40.0 40.0 40.0 5 39.22018344807504 40.0 40.0 40.0 40.0 40.0 6 39.27006801306184 40.0 40.0 40.0 40.0 40.0 7 39.29853227673898 40.0 40.0 40.0 40.0 40.0 8 39.32181617754113 40.0 40.0 40.0 40.0 40.0 9 39.3306849144117 40.0 40.0 40.0 40.0 40.0 10-11 39.32618676206521 40.0 40.0 40.0 40.0 40.0 12-13 39.32345514673766 40.0 40.0 40.0 40.0 40.0 14-15 39.33086794650471 40.0 40.0 40.0 40.0 40.0 16-17 39.47206334019704 40.0 40.0 40.0 40.0 40.0 18-19 39.48256549983707 40.0 40.0 40.0 40.0 40.0 20-21 39.49231611861035 40.0 40.0 40.0 40.0 40.0 22-23 39.49604054438181 40.0 40.0 40.0 40.0 40.0 24-25 39.493932902098635 40.0 40.0 40.0 40.0 40.0 26-27 39.486206729202635 40.0 40.0 40.0 40.0 40.0 28-29 39.489237851591476 40.0 40.0 40.0 40.0 40.0 30-31 39.47719860382749 40.0 40.0 40.0 40.0 40.0 32-33 39.48352480104106 40.0 40.0 40.0 40.0 40.0 34-35 39.47788417703137 40.0 40.0 40.0 40.0 40.0 36-37 39.46504349051767 40.0 40.0 40.0 40.0 40.0 38-39 39.4614391469039 40.0 40.0 40.0 40.0 40.0 40-41 39.44783397974734 40.0 40.0 40.0 40.0 40.0 42-43 39.44669562566109 40.0 40.0 40.0 40.0 40.0 44-45 39.42774522266883 40.0 40.0 40.0 40.0 40.0 46-47 39.421853441782986 40.0 40.0 40.0 40.0 40.0 48-49 39.41495618849373 40.0 40.0 40.0 40.0 40.0 50-51 39.4249272486886 40.0 40.0 40.0 40.0 40.0 52-53 39.410536267025414 40.0 40.0 40.0 40.0 40.0 54-55 39.389512745459356 40.0 40.0 40.0 40.0 40.0 56-57 39.3867734647059 40.0 40.0 40.0 40.0 40.0 58-59 39.372261350935545 40.0 40.0 40.0 40.0 40.0 60-61 39.33796723302403 40.0 40.0 40.0 40.0 40.0 62-63 39.39345841206346 40.0 40.0 40.0 40.0 40.0 64-65 39.39901849564472 40.0 40.0 40.0 40.0 40.0 66-67 39.385767089536955 40.0 40.0 40.0 40.0 40.0 68-69 39.388521056798055 40.0 40.0 40.0 40.0 40.0 70-71 39.38485741624694 40.0 40.0 40.0 40.0 40.0 72-73 39.37890766294623 40.0 40.0 40.0 40.0 40.0 74-75 39.385397582374864 40.0 40.0 40.0 40.0 40.0 76-77 39.37551315801841 40.0 40.0 40.0 40.0 40.0 78-79 39.376320553760976 40.0 40.0 40.0 40.0 40.0 80-81 39.35220210570495 40.0 40.0 40.0 40.0 40.0 82-83 39.3534638292197 40.0 40.0 40.0 40.0 40.0 84-85 39.35417383038563 40.0 40.0 40.0 40.0 40.0 86-87 39.36899153023617 40.0 40.0 40.0 40.0 40.0 88-89 39.35430547218388 40.0 40.0 40.0 40.0 40.0 90-91 39.451102243708036 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21148828336734482 1101 2 0.30924571722752603 1101 3 0.23996809891229276 1101 4 0.1861524319535448 1101 5 0.11623635031703827 1101 6 0.10338741167072385 1101 7 0.08211351718434656 1101 8 0.14990891356146818 1101 9 0.07129363093610408 1101 10-11 0.003303400634088405 1101 12-13 0.05762707411123813 1101 14-15 -0.03269717244944559 1101 16-17 0.06621548877327399 1101 18-19 0.02840076265642466 1101 20-21 0.0841947996826562 1101 22-23 0.04188810705732493 1101 24-25 -0.04057765088303 1101 26-27 0.13900055687243196 1101 28-29 0.046295211645350776 1101 30-31 0.08908498565475043 1101 32-33 0.11261576854394661 1101 34-35 0.08205525149650583 1101 36-37 0.16743527086038057 1101 38-39 0.10088518737545371 1101 40-41 0.09037894313283346 1101 42-43 0.09856729144237164 1101 44-45 0.07969366541093592 1101 46-47 0.018232086207902398 1101 48-49 -0.01614644851817104 1101 50-51 0.09023873549627837 1101 52-53 -0.04962738209813011 1101 54-55 0.025957806128907635 1101 56-57 -0.030344630562630925 1101 58-59 0.06836656207784131 1101 60-61 0.048451875840342495 1101 62-63 0.0950040346896941 1101 64-65 0.07278960288007141 1101 66-67 -0.011741739594775424 1101 68-69 0.03890999700364972 1101 70-71 0.003933158819911853 1101 72-73 0.10869380793443639 1101 74-75 0.17262176777636284 1101 76-77 0.42427898052063284 1101 78-79 0.2546807447017301 1101 80-81 0.3490098905555641 1101 82-83 0.4972625122990664 1101 84-85 0.2579731121731328 1101 86-87 0.5285693385086034 1101 88-89 0.486573376685314 1101 90-91 -0.2375656696711843 1102 1 0.32800077672194305 1102 2 0.12936267629185494 1102 3 0.20173311751941725 1102 4 -0.1684674510873876 1102 5 0.02617787078487055 1102 6 0.02761920327093037 1102 7 0.06318741404771089 1102 8 -0.08060676958579194 1102 9 0.04158602859692451 1102 10-11 -0.08268277693103698 1102 12-13 -0.1360146058462277 1102 14-15 0.002783954610627859 1102 16-17 -0.0030561752352369354 1102 18-19 -0.029812953452029944 1102 20-21 -0.09674087283196542 1102 22-23 -0.034486161948528604 1102 24-25 -0.014123637060592387 1102 26-27 -0.028899496290776483 1102 28-29 -0.11111042365502755 1102 30-31 -0.034997948954497815 1102 32-33 -0.04045589267337846 1102 34-35 -0.0224473682009787 1102 36-37 -0.04433977728275096 1102 38-39 -0.025844343212980903 1102 40-41 -0.07749439340507536 1102 42-43 0.045063113227321594 1102 44-45 -0.048280339472881906 1102 46-47 -0.03307941468642639 1102 48-49 -0.026657835699339216 1102 50-51 0.05950057113454932 1102 52-53 0.06698850757248209 1102 54-55 0.09676940759235464 1102 56-57 -0.02912126451495567 1102 58-59 0.02426689782075897 1102 60-61 -0.06006787504748701 1102 62-63 -0.1724351878718693 1102 64-65 0.033626744209122705 1102 66-67 -0.046695548142032806 1102 68-69 -0.05849680783484956 1102 70-71 0.05944382362584122 1102 72-73 0.12664525004036165 1102 74-75 0.10004664090175197 1102 76-77 -0.12704149903240136 1102 78-79 -0.18179828710237445 1102 80-81 -0.3005417446680667 1102 82-83 -0.29044888354758314 1102 84-85 -0.21416434773200166 1102 86-87 -0.13750901965813966 1102 88-89 -0.9307333309439727 1102 90-91 0.11798988588436998 1103 1 0.3831933974490198 1103 2 0.24107184612365273 1103 3 0.15711022006961173 1103 4 0.22953580447419597 1103 5 0.1121246966123266 1103 6 0.07111350603815936 1103 7 -0.014077914221410026 1103 8 -0.08766048635193613 1103 9 -0.025673873736224095 1103 10-11 0.007018796590394061 1103 12-13 -0.04880896287281189 1103 14-15 1.1439411144209544E-4 1103 16-17 0.04767632612124828 1103 18-19 0.008385635804621927 1103 20-21 0.04993302841599956 1103 22-23 0.045806838594067756 1103 24-25 0.028426552847165 1103 26-27 -0.006512084462244161 1103 28-29 0.008010574717197017 1103 30-31 0.06413390128966512 1103 32-33 0.07599688422561002 1103 34-35 0.030947767318060926 1103 36-37 0.0015147108950301913 1103 38-39 0.021410408912394985 1103 40-41 0.06142073006257931 1103 42-43 0.030582004264751106 1103 44-45 -0.03451763323416657 1103 46-47 -0.03754846404996215 1103 48-49 -0.10425649612517418 1103 50-51 -0.06661070410723369 1103 52-53 0.10474329093221257 1103 54-55 -0.04172068619801905 1103 56-57 -0.020913837792299717 1103 58-59 0.0810531345056944 1103 60-61 0.13927827880306154 1103 62-63 -0.11834226817079241 1103 64-65 -0.1503231264278284 1103 66-67 0.028610132495764162 1103 68-69 -0.27693950856456695 1103 70-71 -0.15701331417113096 1103 72-73 -0.31703898928476093 1103 74-75 -0.09946043800304238 1103 76-77 -0.40598750844895193 1103 78-79 -0.04574825186629994 1103 80-81 -0.5207125438727473 1103 82-83 -0.43137144007625494 1103 84-85 -0.25548066322227214 1103 86-87 -0.5896872959854278 1103 88-89 0.33419242430436213 1103 90-91 0.5624343303288128 1104 1 0.2748887301766416 1104 2 0.1982507906174007 1104 3 0.23028876542675647 1104 4 0.16851622052158888 1104 5 0.026387002886650635 1104 6 -0.01889284863672458 1104 7 0.0759820087872356 1104 8 0.027262496038787276 1104 9 -0.03371332523850157 1104 10-11 0.01068612673412872 1104 12-13 0.0189812383235477 1104 14-15 -0.021544543660006354 1104 16-17 -0.034557368360687235 1104 18-19 0.014413123712351705 1104 20-21 0.001405895004587876 1104 22-23 0.050223600230744125 1104 24-25 0.0054189534962887365 1104 26-27 -0.014760557502143001 1104 28-29 0.07917190468237578 1104 30-31 0.024679773114300474 1104 32-33 0.016729279788918916 1104 34-35 -0.011262204074235171 1104 36-37 0.06929784836723485 1104 38-39 -0.018734170584821186 1104 40-41 -0.06998922397751528 1104 42-43 -0.018200769004202755 1104 44-45 0.013891632012395405 1104 46-47 0.0015884265866077385 1104 48-49 0.12610221708643365 1104 50-51 -0.1391038871235395 1104 52-53 -0.01946764417720459 1104 54-55 -0.007017487107134457 1104 56-57 -0.07978535365500505 1104 58-59 0.1438034472224956 1104 60-61 -0.014014030711827274 1104 62-63 -0.13587908812074545 1104 64-65 0.08854903240854384 1104 66-67 -0.3134565149346642 1104 68-69 -0.013225058251975952 1104 70-71 -0.3039389408930262 1104 72-73 -0.297320815707252 1104 74-75 -0.24482528999309494 1104 76-77 -0.05110421828030809 1104 78-79 -0.1424598264883059 1104 80-81 0.02865773077912337 1104 82-83 0.20372158417935537 1104 84-85 -0.03110830332967396 1104 86-87 0.023304872392657217 1104 88-89 0.2368839356915231 1104 90-91 0.5624343303288128 1201 1 0.31193148958004 1201 2 0.36320678022256914 1201 3 0.08005423677325041 1201 4 0.21570310187730257 1201 5 0.1829533537962007 1201 6 0.0626625077346219 1201 7 0.03531266388115739 1201 8 0.17298416978795927 1201 9 0.10631620847700418 1201 10-11 0.04708404518754605 1201 12-13 0.03725654671873002 1201 14-15 -0.03960112200496013 1201 16-17 0.052786596250449236 1201 18-19 0.08332495460926737 1201 20-21 0.024791438960171774 1201 22-23 0.10162309854447926 1201 24-25 0.10664918106065358 1201 26-27 0.08359883815292335 1201 28-29 0.043346604992542836 1201 30-31 0.08239045877367346 1201 32-33 0.041389655691816074 1201 34-35 0.04583636589169515 1201 36-37 0.10732930757594517 1201 38-39 0.039444721915430137 1201 40-41 0.061383464400407206 1201 42-43 0.05678225824096472 1201 44-45 0.03037993757207147 1201 46-47 0.12388715493115399 1201 48-49 0.18091662758375548 1201 50-51 0.04066154068912908 1201 52-53 -0.1332341546207232 1201 54-55 0.0071413143505694165 1201 56-57 0.05887002506793948 1201 58-59 -0.13028638321017638 1201 60-61 0.021852893027535458 1201 62-63 0.07652294464104159 1201 64-65 -0.11397339142183682 1201 66-67 0.0945016387947959 1201 68-69 0.05122398952185847 1201 70-71 -0.02670673390605316 1201 72-73 0.011439601964696067 1201 74-75 0.08546055053404444 1201 76-77 -0.21138009041576566 1201 78-79 0.09342788768901045 1201 80-81 0.315557326479734 1201 82-83 0.27508988031335946 1201 84-85 0.04543568505219753 1201 86-87 0.20555346549272713 1201 88-89 0.5246686147805519 1201 90-91 -0.10423233633785856 1202 1 0.2384397199297652 1202 2 0.19310721550549914 1202 3 0.22487436985975506 1202 4 0.2864690997759354 1202 5 0.08511275740854529 1202 6 0.09875296653284238 1202 7 0.16838816295555858 1202 8 0.005351208363030935 1202 9 0.0371882753204531 1202 10-11 0.08385144399677813 1202 12-13 0.0027476159137052036 1202 14-15 0.13279643640800742 1202 16-17 -0.05972353533508823 1202 18-19 -0.005837500986878297 1202 20-21 -0.008748361910392077 1202 22-23 -0.05351674235210169 1202 24-25 -0.011308991751668884 1202 26-27 0.0612378034803811 1202 28-29 0.014281712812625358 1202 30-31 0.031790068520010095 1202 32-33 0.03939419170306735 1202 34-35 0.015363705174060271 1202 36-37 -0.09970402412684365 1202 38-39 0.08954507953458801 1202 40-41 -0.0061311808412156665 1202 42-43 0.04054640268491738 1202 44-45 0.004644411952000382 1202 46-47 0.056601967949681864 1202 48-49 0.0717911654156893 1202 50-51 0.13182217874722824 1202 52-53 0.04833695162919582 1202 54-55 0.05613667915974929 1202 56-57 0.07320118462696712 1202 58-59 0.06902442445297652 1202 60-61 0.25250562721084435 1202 62-63 0.02886699832131967 1202 64-65 -0.04447052600745138 1202 66-67 0.025282127993008885 1202 68-69 0.010403173228162643 1202 70-71 -0.17713736206069797 1202 72-73 0.07717925608957188 1202 74-75 0.08298701864770663 1202 76-77 0.16216458368090514 1202 78-79 0.03644596701271752 1202 80-81 0.07872977851074836 1202 82-83 -0.023984983462995046 1202 84-85 -0.1108763012501015 1202 86-87 -0.1646094224667678 1202 88-89 -1.173356936903538 1202 90-91 -0.16483839694391378 1203 1 0.2403646850228256 1203 2 0.24951121029221213 1203 3 0.1202271477935355 1203 4 0.1997565876964842 1203 5 -0.07360293841598065 1203 6 0.0648733872364815 1203 7 0.15726926247082673 1203 8 0.04818458656057345 1203 9 0.12289085853065274 1203 10-11 0.07793663048018828 1203 12-13 -0.0287359240854812 1203 14-15 -0.07338252724810701 1203 16-17 0.0704294742142153 1203 18-19 0.10733601892993505 1203 20-21 0.05442496434795174 1203 22-23 0.06582431537167821 1203 24-25 0.026263170232013522 1203 26-27 -0.08987631364038151 1203 28-29 0.04884006501152527 1203 30-31 0.027061413071336915 1203 32-33 0.008374648476959123 1203 34-35 0.09264612792417637 1203 36-37 0.01776318360727913 1203 38-39 0.0038038011639400793 1203 40-41 0.09456332668028722 1203 42-43 0.03902278596473252 1203 44-45 0.03839457007730118 1203 46-47 0.007623035608830264 1203 48-49 -0.0016732297090982229 1203 50-51 -0.04339367084597967 1203 52-53 0.0852855899229823 1203 54-55 0.006607956456981867 1203 56-57 0.07330408244853714 1203 58-59 -0.0038974421681885474 1203 60-61 0.07479972117199196 1203 62-63 -0.09595716060529469 1203 64-65 0.16948179990215095 1203 66-67 0.01173148188988904 1203 68-69 -0.057536396406426604 1203 70-71 -0.18627609680506652 1203 72-73 0.07817497042554322 1203 74-75 0.14851191727927926 1203 76-77 -0.00216273154359925 1203 78-79 0.2209547754379102 1203 80-81 -0.4158915062447619 1203 82-83 -0.38358001805802644 1203 84-85 -0.31505565489614185 1203 86-87 -0.85000209006283 1203 88-89 -1.1004039752310604 1203 90-91 0.14138169874986772 1204 1 0.3870400374534668 1204 2 0.09787045874455202 1204 3 0.05870944750574125 1204 4 0.110757143011881 1204 5 -0.08622500988418835 1204 6 0.007323392631043646 1204 7 0.04490187676163515 1204 8 0.024026564607957823 1204 9 -0.01105381033340791 1204 10-11 -0.02367190989095036 1204 12-13 -0.07848795449494617 1204 14-15 -0.02703303514183375 1204 16-17 -0.14677213507106757 1204 18-19 -0.03725984434282026 1204 20-21 -0.18830433273232927 1204 22-23 -0.1317665625576936 1204 24-25 -0.03403432599480283 1204 26-27 -0.048284127559981016 1204 28-29 -0.13896515120464414 1204 30-31 -0.0734739820976813 1204 32-33 -0.14478039010687382 1204 34-35 -0.13073885673779984 1204 36-37 -0.15406498165795313 1204 38-39 -0.11305469460607753 1204 40-41 -0.14001399709646023 1204 42-43 -0.06002021995333706 1204 44-45 -0.2697809918262024 1204 46-47 -0.06200305660708949 1204 48-49 -0.20926905000938234 1204 50-51 -0.16104296564876108 1204 52-53 -0.2011583033131643 1204 54-55 -0.05697230142951781 1204 56-57 0.0016401752850683238 1204 58-59 -0.11282689758554909 1204 60-61 -0.22726475226907183 1204 62-63 0.013701036773973385 1204 64-65 0.152506010103572 1204 66-67 -0.005630004643670361 1204 68-69 -0.06968291504185231 1204 70-71 0.07501054042939614 1204 72-73 -0.033623145554990685 1204 74-75 -0.10665284526400143 1204 76-77 -0.10436558244175131 1204 78-79 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54-55 0.11924857432536129 2401 56-57 0.058139925828719186 2401 58-59 0.20350247794012688 2401 60-61 0.19318283879342601 2401 62-63 0.04847011029232817 2401 64-65 0.15189347588994195 2401 66-67 0.16150996535375128 2401 68-69 0.16786428220422067 2401 70-71 0.018852247077425943 2401 72-73 0.032107614703811294 2401 74-75 0.05799135359072238 2401 76-77 -0.027344491175419705 2401 78-79 0.10537592410062047 2401 80-81 0.05514487914550159 2401 82-83 0.4945989066870169 2401 84-85 0.10416992202249276 2401 86-87 0.02388422548586533 2401 88-89 0.09976018987212854 2401 90-91 -0.00899424109975655 2402 1 0.21703800393632378 2402 2 0.21615985215834854 2402 3 0.1408762497402165 2402 4 0.2670075168201862 2402 5 0.1680745331725504 2402 6 0.17611727772536057 2402 7 0.19600191982434723 2402 8 0.11478796725631213 2402 9 0.00874905812195692 2402 10-11 -0.002888168458639484 2402 12-13 0.0776054198277123 2402 14-15 0.13596270942448996 2402 16-17 0.04710428689570989 2402 18-19 0.03348935720266155 2402 20-21 0.006272605987028612 2402 22-23 -0.013141489291271569 2402 24-25 0.008706914929135223 2402 26-27 0.04423753836903188 2402 28-29 0.03683726569028778 2402 30-31 0.048811422033949725 2402 32-33 6.978695434938231E-4 2402 34-35 -0.022775962775618552 2402 36-37 0.003915347365612831 2402 38-39 -0.02665492838126937 2402 40-41 0.060845148607498345 2402 42-43 0.10299258483333773 2402 44-45 0.05753420563400624 2402 46-47 -0.01977619646348927 2402 48-49 0.037939063408188645 2402 50-51 0.0855115299547009 2402 52-53 0.09603916021338677 2402 54-55 0.07713206140581264 2402 56-57 0.11283402327470782 2402 58-59 8.505236351439294E-4 2402 60-61 0.034835761358586126 2402 62-63 0.12001458245231333 2402 64-65 0.011582364759590291 2402 66-67 0.040724043840732804 2402 68-69 0.07813298704859051 2402 70-71 0.19017677132892885 2402 72-73 0.1960164833049376 2402 74-75 0.3270391904765333 2402 76-77 0.20144260120001434 2402 78-79 0.007915130843805684 2402 80-81 0.2424659109565539 2402 82-83 0.13106337634188492 2402 84-85 -0.14569329179106916 2402 86-87 0.2758751727709594 2402 88-89 -0.20358618127085037 2402 90-91 -1.0016682337737493 2403 1 -0.4651645419198758 2403 2 -0.3583847546462948 2403 3 -0.014741699813441755 2403 4 0.03194359880906461 2403 5 0.10120619884332882 2403 6 0.14615005154976046 2403 7 -0.016178037442927007 2403 8 0.050710387449335315 2403 9 0.06923577657488522 2403 10-11 0.052401816512485766 2403 12-13 0.022338068713224857 2403 14-15 0.10463914939044372 2403 16-17 0.04793042266262404 2403 18-19 0.09742887048420101 2403 20-21 0.03958624786509546 2403 22-23 0.13258999168723307 2403 24-25 -0.003037793275183276 2403 26-27 0.06219806944984896 2403 28-29 0.03370417408654447 2403 30-31 0.052963603032367246 2403 32-33 0.05784621822637348 2403 34-35 0.05126891755775631 2403 36-37 -0.0020676689938099457 2403 38-39 0.054752461298839705 2403 40-41 -0.01745084242689643 2403 42-43 -0.008640709778539701 2403 44-45 0.06559019104282271 2403 46-47 0.04397093556418952 2403 48-49 0.12203697486610565 2403 50-51 0.005175390542305536 2403 52-53 0.006594681157729099 2403 54-55 0.04207906768377967 2403 56-57 0.0708929540574843 2403 58-59 -0.0967763212992736 2403 60-61 -0.05171176330777172 2403 62-63 0.1723499942620066 2403 64-65 0.0668986216879901 2403 66-67 0.13107339752878033 2403 68-69 0.22815817005797356 2403 70-71 0.172271846245593 2403 72-73 0.026131159512402746 2403 74-75 0.12731170724035223 2403 76-77 0.23897666124310746 2403 78-79 0.10867378788371695 2403 80-81 0.21433782848643546 2403 82-83 0.22907290635960464 2403 84-85 0.2821911505971926 2403 86-87 -0.17661436667510344 2403 88-89 0.32804803106319014 2403 90-91 0.22910099699548425 2404 1 -0.3110541657060395 2404 2 -0.1989197253974524 2404 3 -0.29949481984860427 2404 4 -0.3249613353058862 2404 5 -0.25612022874100404 2404 6 -0.22127198761316436 2404 7 -0.06843535976797455 2404 8 0.02323800510642826 2404 9 -0.027559355681333386 2404 10-11 -0.0051314800666233396 2404 12-13 0.03032508896232855 2404 14-15 0.008662276456121276 2404 16-17 -0.01940344006650463 2404 18-19 0.03985117659299675 2404 20-21 -0.04475234739270917 2404 22-23 0.08807409941646682 2404 24-25 -0.0220139912579711 2404 26-27 -0.02452561685335297 2404 28-29 0.03026253941123258 2404 30-31 0.03311631854619179 2404 32-33 -0.024888164943988045 2404 34-35 -0.028829311811357172 2404 36-37 -0.017495977915025662 2404 38-39 0.02363583744678266 2404 40-41 0.030565212473888437 2404 42-43 -0.0035797249553368715 2404 44-45 0.02579455497769345 2404 46-47 0.004620382546278279 2404 48-49 -0.01817494669202091 2404 50-51 0.11417353679505737 2404 52-53 -0.037310339537576453 2404 54-55 0.07537924366675952 2404 56-57 0.09521328628225945 2404 58-59 0.029328225379543937 2404 60-61 -0.03870451929739005 2404 62-63 0.08519072494682689 2404 64-65 -0.03214321828252764 2404 66-67 -0.051363700809922364 2404 68-69 0.03767617710378346 2404 70-71 0.11715973558846571 2404 72-73 0.15446685505067137 2404 74-75 0.09260244027406372 2404 76-77 0.1779465017571269 2404 78-79 0.15491573835527817 2404 80-81 -0.0030566707376351587 2404 82-83 0.24775003226855574 2404 84-85 0.15834541388697687 2404 86-87 0.34448948528770984 2404 88-89 0.20336029347222961 2404 90-91 0.5624343303288128 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 1.0 30 16.0 31 373.0 32 2672.0 33 4966.0 34 6767.0 35 8332.0 36 12213.0 37 19106.0 38 51561.0 39 309356.0 40 305822.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.252936502764214 13.682834129018929 28.516280933635247 30.547948434581613 2 26.1082869496005 19.440122691559964 28.13558193904803 26.316008419791505 3 25.591704694673584 20.046895430954034 22.682163512803342 31.67923636156904 4 26.099683160354136 21.895907430132354 21.220629935453456 30.78377947406005 5 26.678868806200907 22.048988817016436 21.034547307556313 30.23759506922634 6 26.692596213176923 25.016881937366975 23.836463598105894 24.454058251350208 7 24.804453780929997 24.772977807358725 23.922155896198618 26.50041251551266 8 23.75479245963241 26.085539771348543 24.587033840138105 25.57263392888094 9 23.629720529406463 26.178581085297115 24.90623071749967 25.285467667796752 10-11 23.45556271969051 25.16559551294051 24.751346741820754 26.62749502554823 12-13 23.791953520941227 24.876488002384963 24.58252736815103 26.74903110852278 14-15 24.17645957694628 24.44130146910987 24.494269847542586 26.887969106401272 16-17 24.152956592275213 24.23441973973391 24.27802852250116 27.334595145489715 18-19 24.32843167841816 24.582874019842343 24.034401713845963 27.054292587893535 20-21 24.36947523866969 24.72825973917927 23.873347338061663 27.028917684089382 22-23 24.26235986605379 24.26804495379133 24.136941284136526 27.33265389601836 24-25 24.212026040475052 24.668912969626376 24.12460048392576 26.994460505972807 26-27 24.4589113750286 24.732558220151557 23.982750611840235 26.82577979297961 28-29 24.471806817945467 24.410657459597747 24.053120905176897 27.064414817279893 30-31 24.66194480188135 24.501167524515242 24.02874994453565 26.808137729067756 32-33 24.76129226920552 24.506736795731626 23.909232629280396 26.82273830578246 34-35 24.66688382749622 24.183395980572644 24.101777668790696 27.04794252314044 36-37 24.677717931764384 24.520143437680467 24.11156061139817 26.69057801915698 38-39 24.92710385544907 24.52775457622485 23.98805687369516 26.557084694630916 40-41 24.865798971160245 24.17347450994804 24.08093071660468 26.87979580228703 42-43 24.929924755740874 24.35908188408164 24.084166217155126 26.626827143022357 44-45 25.06025136239846 24.39464853450112 23.98646842115632 26.558631681944107 46-47 25.054755505636006 24.149484536082475 23.9872237153516 26.80853624292992 48-49 25.139794364216094 24.36218821326407 23.970634554232877 26.52738286828696 50-51 25.262015529177535 24.299980533158326 23.890244314691405 26.547759622972738 52-53 25.29715270910981 24.019548185897925 23.952105676833753 26.731193428158516 54-55 25.354226513551115 24.28214948046778 23.926917331195373 26.436706674785732 56-57 25.429859005330602 24.33733726248449 23.676696582950925 26.55610714923398 58-59 25.497962268224878 23.971350300936546 23.902188874794202 26.628498556044374 60-61 25.72021172760718 24.03558825737668 23.745964411742623 26.498235603273518 62-63 25.83588096029066 24.188508975917042 23.54680134396959 26.42880871982271 64-65 25.818287904518844 23.827357855089517 23.671764560781003 26.682589679610636 66-67 25.90880312521048 23.93803461978851 23.51289822859837 26.640264026402637 68-69 25.983541426697137 23.930839498112068 23.4291363560768 26.656482719113995 70-71 26.2418327358514 23.71819696652088 23.28367541986577 26.75629487776195 72-73 26.188833862713594 23.889850355421363 23.316963160916455 26.60435262094859 74-75 26.333257205055567 23.719951601852348 23.21070201610356 26.736089176988525 76-77 26.483914684720837 23.617581880681918 22.954509762864472 26.94399367173277 78-79 26.756039758055962 23.728909483216015 22.864419369672103 26.650631389055924 80-81 26.911296197154666 23.531476816949123 22.489709586540403 27.067517399355808 82-83 27.215852190253287 23.023258430759192 22.37400276805707 27.38688661093045 84-85 27.848196657070943 22.776530166242143 21.811933864866088 27.56333931182083 86-87 28.766044914828015 21.654894929041568 21.23942183639107 28.339638319739347 88-89 27.814979256132176 20.853046073222213 21.27884125482204 30.05313341582357 90-91 25.41856925418569 22.66988987375772 22.848956934371923 29.06258393768466 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.5 10 2.0 11 2.0 12 3.0 13 4.5 14 6.5 15 11.5 16 18.5 17 23.5 18 30.5 19 46.5 20 81.5 21 127.5 22 179.0 23 253.5 24 371.5 25 522.0 26 713.0 27 1047.0 28 1520.5 29 2073.5 30 2793.5 31 3712.0 32 4863.5 33 6200.0 34 8006.0 35 10283.5 36 13077.5 37 16066.0 38 19124.0 39 22745.0 40 27095.5 41 31937.5 42 36453.5 43 40799.0 44 44955.0 45 48815.0 46 52383.0 47 55439.5 48 57801.0 49 59540.5 50 60468.0 51 60176.5 52 59060.0 53 57349.5 54 55032.0 55 52371.0 56 49579.0 57 46563.5 58 43289.5 59 39830.5 60 36765.0 61 33966.0 62 31401.0 63 28803.0 64 25867.0 65 23127.0 66 20436.5 67 18092.5 68 15918.0 69 13556.0 70 11357.5 71 9453.5 72 7706.5 73 6053.0 74 4654.0 75 3620.5 76 2784.5 77 2027.0 78 1440.5 79 1010.5 80 699.5 81 489.0 82 337.5 83 231.5 84 166.0 85 118.5 86 74.5 87 40.5 88 28.5 89 18.5 90 8.0 91 5.0 92 2.5 93 0.5 94 0.0 95 0.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.54642706101763E-4 2 0.0037438382661869004 3 8.319640591526446E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 9891.0 32-33 22092.0 34-35 25351.0 36-37 27383.0 38-39 30007.0 40-41 31932.0 42-43 33036.0 44-45 34207.0 46-47 34969.0 48-49 34655.0 50-51 34736.0 52-53 34032.0 54-55 33245.0 56-57 31584.0 58-59 30372.0 60-61 28912.0 62-63 27455.0 64-65 25758.0 66-67 23573.0 68-69 22051.0 70-71 20267.0 72-73 18319.0 74-75 17331.0 76-77 15729.0 78-79 14049.0 80-81 12875.0 82-83 11543.0 84-85 10503.0 86-87 9463.0 88-89 8378.0 90-91 7487.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.88971528749259 #Duplication Level Percentage of deduplicated Percentage of total 1 99.8925181651071 99.7823519886323 2 0.10486594203247988 0.20950058185958237 3 0.002307146643866741 0.0069138066414702855 4 3.0874621654032087E-4 0.0012336228666521274 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.00180258879483073 0.0 0.0 0.0 0.0 2 0.00180258879483073 0.0 0.0 0.0 0.0 3 0.00180258879483073 0.0 0.0 0.0 0.0 4 0.00180258879483073 1.3866067652544076E-4 0.0 0.0 0.0 5 0.00180258879483073 1.3866067652544076E-4 0.0 0.0 0.0 6 0.0019412494713561708 1.3866067652544076E-4 0.0 0.0 0.0 7 0.0024958921774579338 1.3866067652544076E-4 0.0 0.0 0.0 8 0.009706247356780854 1.3866067652544076E-4 0.0 0.0 1.3866067652544076E-4 9 0.009844908033306295 1.3866067652544076E-4 0.0 0.0 1.3866067652544076E-4 10-11 0.009844908033306295 1.3866067652544076E-4 0.0 0.0 2.773213530508815E-4 12-13 0.009844908033306295 1.3866067652544076E-4 0.0 0.0 2.773213530508815E-4 14-15 0.009844908033306295 1.3866067652544076E-4 0.0 0.0 2.773213530508815E-4 16-17 0.009844908033306295 2.0799101478816113E-4 0.0 0.0 2.773213530508815E-4 18-19 0.009914238371569014 2.773213530508815E-4 0.0 0.0 3.466516913136019E-4 20-21 0.009983568709831735 2.773213530508815E-4 0.0 0.0 4.159820295763223E-4 22-23 0.009983568709831735 2.773213530508815E-4 0.0 0.0 4.159820295763223E-4 24-25 0.009983568709831735 2.773213530508815E-4 0.0 0.0 4.159820295763223E-4 26-27 0.009983568709831735 2.773213530508815E-4 0.0 0.0 4.159820295763223E-4 28-29 0.010260890062882617 2.773213530508815E-4 0.0 0.0 4.159820295763223E-4 30-31 0.010399550739408058 2.773213530508815E-4 0.0 0.0 4.159820295763223E-4 32-33 0.010884863107247102 2.773213530508815E-4 0.0 0.0 4.159820295763223E-4 34-35 0.01102352378377254 2.773213530508815E-4 0.0 0.0 4.159820295763223E-4 36-37 0.011439505813348863 2.773213530508815E-4 0.0 0.0 4.159820295763223E-4 38-39 0.011924818181187905 2.773213530508815E-4 0.0 0.0 4.159820295763223E-4 40-41 0.012063478857713347 2.773213530508815E-4 0.0 0.0 6.239730443644834E-4 42-43 0.012202139534238788 2.773213530508815E-4 0.0 0.0 6.933033826272038E-4 44-45 0.01247946088728967 2.773213530508815E-4 0.0 0.0 6.933033826272038E-4 46-47 0.01261812156381511 2.773213530508815E-4 0.0 0.0 6.933033826272038E-4 48-49 0.012687451902077831 2.773213530508815E-4 0.0 0.0 6.933033826272038E-4 50-51 0.01275678224034055 2.773213530508815E-4 0.0 0.0 6.933033826272038E-4 52-53 0.01275678224034055 2.773213530508815E-4 0.0 0.0 6.933033826272038E-4 54-55 0.012895442916865991 2.773213530508815E-4 0.0 0.0 6.933033826272038E-4 56-57 0.013242094608179593 2.773213530508815E-4 0.0 0.0 6.933033826272038E-4 58-59 0.013311424946442314 2.773213530508815E-4 0.0 0.0 6.933033826272038E-4 60-61 0.013311424946442314 2.773213530508815E-4 0.0 0.0 6.933033826272038E-4 62-63 0.013450085622967754 2.773213530508815E-4 0.0 0.0 6.933033826272038E-4 64-65 0.013588746299493195 2.773213530508815E-4 0.0 0.0 6.933033826272038E-4 66-67 0.013588746299493195 2.773213530508815E-4 0.0 0.0 6.933033826272038E-4 68-69 0.013727406976018637 2.773213530508815E-4 0.0 0.0 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