FastQCFastQC Report
Mon 8 Jul 2024
AM-AL-4B_S57_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAM-AL-4B_S57_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271232
Sequences flagged as poor quality0
Sequence length61
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGATTGATCTGGGGGGGGGGGGGGG168081.3221819463323767TruSeq Adapter, Index 20 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGATTGATCTGGGGGGGGGGGGGGT22910.1802188742888788TruSeq Adapter, Index 20 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)