##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-4B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 788627 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.3148192998718 40.0 40.0 40.0 40.0 40.0 2 39.20543171867055 40.0 40.0 40.0 40.0 40.0 3 39.17448426188807 40.0 40.0 40.0 40.0 40.0 4 39.08092926060102 40.0 40.0 40.0 40.0 40.0 5 39.22220771036244 40.0 40.0 40.0 40.0 40.0 6 39.27702957164794 40.0 40.0 40.0 40.0 40.0 7 39.29935951977297 40.0 40.0 40.0 40.0 40.0 8 39.32101741380907 40.0 40.0 40.0 40.0 40.0 9 39.34836621114925 40.0 40.0 40.0 40.0 40.0 10-11 39.3344661037474 40.0 40.0 40.0 40.0 40.0 12-13 39.335254816281974 40.0 40.0 40.0 40.0 40.0 14-15 39.341861234778925 40.0 40.0 40.0 40.0 40.0 16-17 39.47746653360841 40.0 40.0 40.0 40.0 40.0 18-19 39.49271835734764 40.0 40.0 40.0 40.0 40.0 20-21 39.500273259728615 40.0 40.0 40.0 40.0 40.0 22-23 39.500460927662886 40.0 40.0 40.0 40.0 40.0 24-25 39.50815277691481 40.0 40.0 40.0 40.0 40.0 26-27 39.49668220844582 40.0 40.0 40.0 40.0 40.0 28-29 39.49889618285958 40.0 40.0 40.0 40.0 40.0 30-31 39.49063560345226 40.0 40.0 40.0 40.0 40.0 32-33 39.48660061255732 40.0 40.0 40.0 40.0 40.0 34-35 39.48727418884478 40.0 40.0 40.0 40.0 40.0 36-37 39.486139517543066 40.0 40.0 40.0 40.0 40.0 38-39 39.46780359330708 40.0 40.0 40.0 40.0 40.0 40-41 39.460319989674126 40.0 40.0 40.0 40.0 40.0 42-43 39.457465931840545 40.0 40.0 40.0 40.0 40.0 44-45 39.434924700556024 40.0 40.0 40.0 40.0 40.0 46-47 39.431009971688994 40.0 40.0 40.0 40.0 40.0 48-49 39.43980231600151 40.0 40.0 40.0 40.0 40.0 50-51 39.43426273455452 40.0 40.0 40.0 40.0 40.0 52-53 39.42605567532813 40.0 40.0 40.0 40.0 40.0 54-55 39.419376458764035 40.0 40.0 40.0 40.0 40.0 56-57 39.403247481135686 40.0 40.0 40.0 40.0 40.0 58-59 39.39228969807161 40.0 40.0 40.0 40.0 40.0 60-61 39.35859100763308 40.0 40.0 40.0 40.0 40.0 62-63 39.407919032387056 40.0 40.0 40.0 40.0 40.0 64-65 39.405668094550165 40.0 40.0 40.0 40.0 40.0 66-67 39.40405098784046 40.0 40.0 40.0 40.0 40.0 68-69 39.39942701494718 40.0 40.0 40.0 40.0 40.0 70-71 39.402356803291674 40.0 40.0 40.0 40.0 40.0 72-73 39.39064894665904 40.0 40.0 40.0 40.0 40.0 74-75 39.38194572135865 40.0 40.0 40.0 40.0 40.0 76-77 39.39141313265992 40.0 40.0 40.0 40.0 40.0 78-79 39.381669139550596 40.0 40.0 40.0 40.0 40.0 80-81 39.37755813753235 40.0 40.0 40.0 40.0 40.0 82-83 39.342752021414015 40.0 40.0 40.0 40.0 40.0 84-85 39.357118304129266 40.0 40.0 40.0 40.0 40.0 86-87 39.35799468224249 40.0 40.0 40.0 40.0 40.0 88-89 39.403984472545964 40.0 40.0 40.0 40.0 40.0 90-91 39.409838018794645 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27560844062617207 1101 2 0.21111650121775227 1101 3 0.22610019330067388 1101 4 0.11856400802876976 1101 5 -0.046016712832994244 1101 6 0.05025869832734742 1101 7 0.13589344982148788 1101 8 -0.0012444732326244434 1101 9 0.08701268409485152 1101 10-11 0.02622406079173345 1101 12-13 0.08308538971347446 1101 14-15 0.13118203020803065 1101 16-17 0.07183607010422577 1101 18-19 0.06912334302855783 1101 20-21 0.08426693430438092 1101 22-23 0.11044917037364854 1101 24-25 0.12382810371769182 1101 26-27 0.10939792802813031 1101 28-29 0.09062979163287821 1101 30-31 0.1532569007246991 1101 32-33 0.08720841169753157 1101 34-35 0.09585158879336575 1101 36-37 0.15093564155847616 1101 38-39 0.09306607000255696 1101 40-41 0.07835244726435775 1101 42-43 0.11345084705944686 1101 44-45 0.12443363569672528 1101 46-47 0.11389782641582258 1101 48-49 0.15591972971854062 1101 50-51 0.1373432173682474 1101 52-53 0.07809956303411525 1101 54-55 0.13065946195045086 1101 56-57 0.26949460932371494 1101 58-59 0.15582806229001278 1101 60-61 0.03556566973633579 1101 62-63 0.09794678148197988 1101 64-65 0.16201277253922797 1101 66-67 0.1069614766936624 1101 68-69 0.06933683224153953 1101 70-71 -0.06441213512619726 1101 72-73 0.20264503230566078 1101 74-75 -0.005070399497618894 1101 76-77 0.1333639510526723 1101 78-79 0.12750442959854524 1101 80-81 0.040227152303423 1101 82-83 -0.15923582323397056 1101 84-85 0.37639384362330475 1101 86-87 0.43391357145309684 1101 88-89 0.12156750794198956 1101 90-91 0.29932047441064924 1102 1 0.3692293676569207 1102 2 0.2551734080557537 1102 3 0.1728647642047605 1102 4 -0.028292348097892273 1102 5 0.08248259877783681 1102 6 0.05943722058526646 1102 7 0.20748592343323935 1102 8 -0.0012444732326244434 1102 9 0.022763028289439546 1102 10-11 -0.0352720383363021 1102 12-13 -0.12434921331543336 1102 14-15 0.007271979726148459 1102 16-17 0.006668562073016915 1102 18-19 0.0021201305457694275 1102 20-21 0.028277948531091113 1102 22-23 -0.02172155014034871 1102 24-25 0.08619616246023298 1102 26-27 -0.051226211485406736 1102 28-29 -0.02501958881686761 1102 30-31 -0.06978119014267037 1102 32-33 0.03295156965727841 1102 34-35 -0.05779185873491599 1102 36-37 -0.023437987776219416 1102 38-39 -0.02009261448720423 1102 40-41 0.054174633839068065 1102 42-43 -0.06523423998437039 1102 44-45 -0.011856469762491884 1102 46-47 -0.07343976414007614 1102 48-49 -0.11152045501867747 1102 50-51 0.01775820517946869 1102 52-53 0.11655179302127294 1102 54-55 0.018708307786319267 1102 56-57 0.04231557429967836 1102 58-59 -0.024141182688182994 1102 60-61 0.0206391245357338 1102 62-63 0.06864958413807187 1102 64-65 0.07170501464145218 1102 66-67 0.11806694411810525 1102 68-69 0.07581424127792502 1102 70-71 0.24053647827347646 1102 72-73 0.13362533591248393 1102 74-75 0.21894820282636118 1102 76-77 0.17879176398298569 1102 78-79 0.07180039681242079 1102 80-81 0.310733365612208 1102 82-83 0.14496409263131937 1102 84-85 -0.22101492417085922 1102 86-87 0.2454725806808753 1102 88-89 0.3836121612909906 1102 90-91 0.7203731059895944 1103 1 0.32200415838682517 1103 2 0.21260975504617363 1103 3 0.19803953557140375 1103 4 0.22977336817669425 1103 5 0.1129818431668781 1103 6 0.15650886611758352 1103 7 -0.004415757724515856 1103 8 0.06629355411227067 1103 9 0.046169739772039975 1103 10-11 -0.010205498277727543 1103 12-13 0.07531894780293413 1103 14-15 0.08679620166036983 1103 16-17 0.0830659138727512 1103 18-19 0.0684278723185443 1103 20-21 0.058752248985854294 1103 22-23 0.0334704980716225 1103 24-25 0.015645837967127818 1103 26-27 0.0038237831347345264 1103 28-29 0.04858700179335074 1103 30-31 0.0791231052662269 1103 32-33 0.05612794755137429 1103 34-35 0.07426368883138679 1103 36-37 0.030795824511812953 1103 38-39 -0.028447342872532033 1103 40-41 0.01890322122601873 1103 42-43 0.061797230330753905 1103 44-45 0.13333767885601588 1103 46-47 0.07565888985678981 1103 48-49 0.0488003787788287 1103 50-51 -0.05590892962846539 1103 52-53 0.06831003479611297 1103 54-55 0.07113430659826037 1103 56-57 -9.359605396994652E-4 1103 58-59 0.08542277995059067 1103 60-61 0.10204427763142121 1103 62-63 0.021219003891559396 1103 64-65 0.09436149527471827 1103 66-67 0.03077189057258778 1103 68-69 -0.07511673079125814 1103 70-71 0.06668955496780171 1103 72-73 0.2788674321048745 1103 74-75 0.10366503136539507 1103 76-77 0.2360048136124675 1103 78-79 0.19609859276166475 1103 80-81 0.020929899582213807 1103 82-83 -0.26549097563105306 1103 84-85 -0.06978938829015391 1103 86-87 -0.2547847533386829 1103 88-89 0.357404135082966 1103 90-91 0.33942072503721477 1104 1 0.33602654802162846 1104 2 0.20992514757114122 1104 3 0.19643308540752713 1104 4 0.03737917795996282 1104 5 -0.00784029725242874 1104 6 0.1252511865202237 1104 7 0.11175368343131709 1104 8 0.007629783962379122 1104 9 0.13265834340816696 1104 10-11 -0.010798928920145556 1104 12-13 0.03230135933079481 1104 14-15 -0.06833367420984615 1104 16-17 -0.07896856770445737 1104 18-19 -0.05688158505405028 1104 20-21 -0.016019588986850408 1104 22-23 0.006505145031148629 1104 24-25 -0.04805342170382687 1104 26-27 0.03207920278916987 1104 28-29 0.021571315006298164 1104 30-31 0.018101685507737386 1104 32-33 -0.061502399793646134 1104 34-35 -0.042188034032854205 1104 36-37 0.04461407606234502 1104 38-39 -0.01985166634617741 1104 40-41 -0.050051931760386026 1104 42-43 -0.049992008182449865 1104 44-45 -0.0798615650274499 1104 46-47 -0.1004582964239944 1104 48-49 0.03275724676162639 1104 50-51 0.052376168785663424 1104 52-53 -0.038600138173698895 1104 54-55 0.004058418863429836 1104 56-57 -0.009766315550756133 1104 58-59 0.06122920580789781 1104 60-61 0.03420643676651025 1104 62-63 -0.1955194113010279 1104 64-65 -0.10796563589217101 1104 66-67 -0.2645772941489142 1104 68-69 -0.042858284188746154 1104 70-71 -0.10641943681071808 1104 72-73 -0.15842020138586577 1104 74-75 0.22162865312439095 1104 76-77 -0.5887429914268907 1104 78-79 0.19835774657773442 1104 80-81 0.1695201613868207 1104 82-83 0.16440558679410344 1104 84-85 0.05134003783926744 1104 86-87 0.4138444387079119 1104 88-89 -0.129673538201601 1104 90-91 -0.0574046717881842 1201 1 0.2270519812930516 1201 2 0.1307716213587966 1201 3 0.11707928412859303 1201 4 0.03595647707202687 1201 5 0.23018454184192905 1201 6 0.16277326040593465 1201 7 0.13130917250552443 1201 8 0.04618896690659113 1201 9 0.03728253680241522 1201 10-11 0.0858250650300505 1201 12-13 0.09975333694320199 1201 14-15 -0.02859112323734081 1201 16-17 -0.023174094797688838 1201 18-19 0.06713272505651702 1201 20-21 0.007862173932338123 1201 22-23 0.08515175095048733 1201 24-25 -0.043096406051617464 1201 26-27 0.03265842155021659 1201 28-29 0.05293306080654503 1201 30-31 0.056157272226428745 1201 32-33 0.012554820549354417 1201 34-35 0.09597044821671119 1201 36-37 -0.024940193495773144 1201 38-39 0.10621867054103262 1201 40-41 -0.015250947834296369 1201 42-43 -0.07056078690062151 1201 44-45 0.017962343141547876 1201 46-47 0.0976051845481436 1201 48-49 -0.0372888053668774 1201 50-51 0.09894841896034023 1201 52-53 4.979444648967046E-5 1201 54-55 0.05552312033955076 1201 56-57 0.13801586800026655 1201 58-59 -7.098079980636385E-4 1201 60-61 -0.03298138041075305 1201 62-63 0.04413364103135109 1201 64-65 -0.12123024042237773 1201 66-67 -0.08359238089116872 1201 68-69 -0.18260847538088143 1201 70-71 0.03491974577431023 1201 72-73 -0.1857680979836971 1201 74-75 -0.15822230643670565 1201 76-77 -0.18925499657429157 1201 78-79 -0.10203872547647563 1201 80-81 0.13862294892083327 1201 82-83 -0.019641798865322357 1201 84-85 -0.4430694228623224 1201 86-87 0.3880792434511875 1201 88-89 -1.1144573367785071 1201 90-91 -1.946293560677077 1202 1 0.18630150049872896 1202 2 0.15637276482621587 1202 3 0.008763047935119062 1202 4 0.13876897330797533 1202 5 0.051535423331650065 1202 6 0.05291045240647918 1202 7 0.0840881436257419 1202 8 0.05482921230240834 1202 9 0.05674717884652125 1202 10-11 -0.004075242919213906 1202 12-13 0.026107289895620056 1202 14-15 -0.006843561336332016 1202 16-17 0.038090544837430684 1202 18-19 0.009187674126444278 1202 20-21 0.039814218964394854 1202 22-23 0.04712021726678017 1202 24-25 0.05321904320500437 1202 26-27 -0.04498815547524515 1202 28-29 0.0678712099792591 1202 30-31 0.06789949419882646 1202 32-33 -0.02710513501550338 1202 34-35 -0.046229508589576085 1202 36-37 0.02622828355789153 1202 38-39 0.01740662469823917 1202 40-41 0.05633921419458687 1202 42-43 0.04413907382056692 1202 44-45 0.06031755288704943 1202 46-47 0.08554294211711522 1202 48-49 0.06585262913997525 1202 50-51 0.061742061726739905 1202 52-53 -0.01502964167286791 1202 54-55 0.13819381792604446 1202 56-57 0.07171721989240609 1202 58-59 0.1236607463044308 1202 60-61 0.05502685303943622 1202 62-63 -0.04808012179771737 1202 64-65 -0.07050672775774558 1202 66-67 0.04601496439210706 1202 68-69 0.08956588336987181 1202 70-71 -0.15699643839993627 1202 72-73 0.07984359521138629 1202 74-75 0.2400433522367038 1202 76-77 -0.07810300471456344 1202 78-79 -0.19855286774350844 1202 80-81 -0.08333465541932128 1202 82-83 -0.31718831839327777 1202 84-85 -0.21262071359135604 1202 86-87 -0.2252659062847897 1202 88-89 0.32396630854169217 1202 90-91 -2.25740467178818 1203 1 0.1759452625711262 1203 2 0.2661735521949993 1203 3 0.1334799571442531 1203 4 0.14490007117170478 1203 5 -0.029989919500508222 1203 6 0.06499486551562228 1203 7 0.03721439491106082 1203 8 0.17964862680498328 1203 9 0.060658396449241536 1203 10-11 0.021501429438629316 1203 12-13 -0.07283434294964053 1203 14-15 0.012126324530427723 1203 16-17 0.0378751235566952 1203 18-19 0.055690641975012056 1203 20-21 0.033585420742866745 1203 22-23 0.047467774277336616 1203 24-25 -0.0019640408589438607 1203 26-27 -0.005761461815517066 1203 28-29 0.011724736321511386 1203 30-31 0.0195105891155265 1203 32-33 -0.03707353143094139 1203 34-35 0.05555917086867623 1203 36-37 -0.013765791418016704 1203 38-39 -0.06375337445687279 1203 40-41 0.01847067146222514 1203 42-43 -0.007584727666134938 1203 44-45 -0.1276841340308792 1203 46-47 -0.07736977199576955 1203 48-49 0.11300346363891123 1203 50-51 0.011396520802570365 1203 52-53 0.053889091099634356 1203 54-55 0.031570025617952524 1203 56-57 -0.019397957051978665 1203 58-59 -0.012618149276001134 1203 60-61 -0.04917857412264226 1203 62-63 -0.030256986908938188 1203 64-65 0.05507336383701045 1203 66-67 0.0876084547574223 1203 68-69 0.15361270562475937 1203 70-71 0.20272095746062035 1203 72-73 0.11827825736462927 1203 74-75 0.017538630695035806 1203 76-77 0.07947705423116247 1203 78-79 0.05388770840792745 1203 80-81 -0.07806940132415008 1203 82-83 0.16591803709482633 1203 84-85 0.2930720215067453 1203 86-87 -0.27639005357576707 1203 88-89 -0.6490404007733304 1203 90-91 -0.946293560677077 1204 1 0.23894020326680732 1204 2 0.1493280976788185 1204 3 -0.011748806498317776 1204 4 -0.0849617399368654 1204 5 -0.20049427191921865 1204 6 -0.10586051438306043 1204 7 -0.013832729723013415 1204 8 -0.08941232637954499 1204 9 -0.046450071042066554 1204 10-11 -0.11290506038318426 1204 12-13 -0.017592600951608972 1204 14-15 -0.18980464865300206 1204 16-17 -0.008222996675051775 1204 18-19 -0.05770975156221425 1204 20-21 -0.05100737127818178 1204 22-23 -0.04629681832145849 1204 24-25 -0.0926965933439945 1204 26-27 -0.07828647654901744 1204 28-29 -0.04059687569972681 1204 30-31 -0.10401887948039956 1204 32-33 -0.12729601847007643 1204 34-35 -0.02239532872911809 1204 36-37 -0.11171834868471109 1204 38-39 -0.15531778742752778 1204 40-41 -0.07755662844566302 1204 42-43 -0.05008105377365979 1204 44-45 -0.06703354972074038 1204 46-47 -0.16199816322277627 1204 48-49 -0.11027208350049733 1204 50-51 -0.06921077491433181 1204 52-53 -0.1555563248212195 1204 54-55 -0.19662155039144125 1204 56-57 -0.17160555323458482 1204 58-59 -0.0664252425619054 1204 60-61 -0.11550004493676624 1204 62-63 -0.09090490139867313 1204 64-65 0.046361349407007424 1204 66-67 -0.026832650895450172 1204 68-69 0.020551648484548934 1204 70-71 -0.08171521699204476 1204 72-73 0.052601894947194694 1204 74-75 -0.15525887077471623 1204 76-77 -0.060034510683962594 1204 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0.05290746404675417 2401 54-55 0.1114956890779979 2401 56-57 0.04395911659862861 2401 58-59 0.12062885689044833 2401 60-61 0.0768214949247934 2401 62-63 0.06269028159289292 2401 64-65 0.0664787787704455 2401 66-67 0.1850199748832395 2401 68-69 0.026866299577967823 2401 70-71 0.23411008268935518 2401 72-73 0.10602133014967308 2401 74-75 0.29837795010382706 2401 76-77 0.08066356760076587 2401 78-79 0.2365654103611874 2401 80-81 0.37269929056980544 2401 82-83 0.41269594561663325 2401 84-85 0.16263999024091902 2401 86-87 0.05926568412915145 2401 88-89 0.5998283775072082 2401 90-91 -0.7539858683693836 2402 1 0.3426791613349067 2402 2 0.2655735695562029 2402 3 0.23144307991346835 2402 4 0.28424412419484923 2402 5 0.18340220362103565 2402 6 0.18338037606255142 2402 7 0.12107409599204999 2402 8 0.14728530795633787 2402 9 0.13328438003780008 2402 10-11 0.1318854693898146 2402 12-13 0.14756353489899965 2402 14-15 0.12758851541170912 2402 16-17 0.0377742754110173 2402 18-19 0.062312152501519336 2402 20-21 0.08610815625520019 2402 22-23 0.02245998726861842 2402 24-25 0.014322296387462075 2402 26-27 0.045649390976826965 2402 28-29 0.09334407669804534 2402 30-31 0.05667632219218177 2402 32-33 0.05768484984226063 2402 34-35 0.08410689187401488 2402 36-37 0.06375816610689355 2402 38-39 0.10939394897435051 2402 40-41 0.04832943215578922 2402 42-43 0.055337763917926 2402 44-45 0.17087871244741137 2402 46-47 0.05905478718007373 2402 48-49 0.15713185088836212 2402 50-51 0.10326366094656692 2402 52-53 0.037751486283340796 2402 54-55 0.15443352268948018 2402 56-57 0.08970678414779343 2402 58-59 0.05186496623505832 2402 60-61 0.3297785600796601 2402 62-63 0.21428974832937797 2402 64-65 0.11082326009700694 2402 66-67 0.03287297252160215 2402 68-69 -0.0367031668968707 2402 70-71 0.13414598500054353 2402 72-73 0.0642005034898645 2402 74-75 0.1641434630594958 2402 76-77 -0.02541431708318953 2402 78-79 0.1593583402404235 2402 80-81 -0.06011830156714382 2402 82-83 0.09766854125919622 2402 84-85 0.27184084850402 2402 86-87 -0.2180333836461017 2402 88-89 -0.3168382891594561 2402 90-91 0.12778051339699914 2403 1 -0.2513510604096325 2403 2 -0.1688573411366363 2403 3 -0.07391569197530856 2403 4 -0.038464149071877785 2403 5 0.041609277568248615 2403 6 0.10616050283266532 2403 7 0.10184573619922332 2403 8 0.10285387899243403 2403 9 0.10584318493491196 2403 10-11 0.14433051630341254 2403 12-13 0.186256747107862 2403 14-15 0.18901520999457233 2403 16-17 0.0718292131505649 2403 18-19 0.12436328824475851 2403 20-21 0.047980262050927536 2403 22-23 0.08787183414014521 2403 24-25 0.09900854363698386 2403 26-27 0.16877751412608433 2403 28-29 0.10938355989625848 2403 30-31 0.12074645080895152 2403 32-33 0.10556625305336809 2403 34-35 0.16871682592362447 2403 36-37 0.11961747064451345 2403 38-39 0.16054979815820758 2403 40-41 0.0544705761298232 2403 42-43 0.1392033266911099 2403 44-45 0.14028469171711322 2403 46-47 0.08121573201583487 2403 48-49 0.11247439821418226 2403 50-51 0.06285160763604125 2403 52-53 0.09475558760337321 2403 54-55 -0.04267388865069677 2403 56-57 0.11302401056656919 2403 58-59 0.010844010272080595 2403 60-61 0.025113792946925173 2403 62-63 0.032385285662897445 2403 64-65 0.22835544493551652 2403 66-67 0.19175734121117927 2403 68-69 0.26620478692070293 2403 70-71 0.20430268406813923 2403 72-73 0.23921012654885487 2403 74-75 0.3197771310706514 2403 76-77 0.07440155609201327 2403 78-79 0.010544947439598218 2403 80-81 0.028432325652531176 2403 82-83 0.2476951223346333 2403 84-85 0.24846377265670583 2403 86-87 0.2067631305030062 2403 88-89 0.4871523211691766 2403 90-91 0.5098467902001218 2404 1 -0.34023358452662933 2404 2 -0.21363957311817927 2404 3 -0.39429557793223324 2404 4 -0.3075924670407275 2404 5 -0.12147739433419957 2404 6 -0.2020884309733333 2404 7 -0.19362364489111883 2404 8 -0.16146752077244741 2404 9 -0.003709693627889976 2404 10-11 0.12697438950957718 2404 12-13 0.04959600195218883 2404 14-15 0.04734826865769293 2404 16-17 0.035164338470274004 2404 18-19 0.013714001951640853 2404 20-21 0.031211361235676804 2404 22-23 0.01633331344194744 2404 24-25 -0.018032034435847777 2404 26-27 0.016858743769702755 2404 28-29 -0.005510423083286753 2404 30-31 0.03995249097748399 2404 32-33 0.04198925075050397 2404 34-35 -0.03358522174512757 2404 36-37 0.0876680100344771 2404 38-39 0.1605660657716399 2404 40-41 -0.010050798517660553 2404 42-43 0.13877462142524877 2404 44-45 0.041262907084835376 2404 46-47 0.043186787815457706 2404 48-49 0.0759844985223097 2404 50-51 0.08834562759857789 2404 52-53 0.019796691584829773 2404 54-55 0.09336045451727415 2404 56-57 0.01936800540494943 2404 58-59 0.05442274068833797 2404 60-61 0.152408519581968 2404 62-63 0.04820000366710531 2404 64-65 -0.01819628438947518 2404 66-67 0.030031823254297763 2404 68-69 -0.024843292238365677 2404 70-71 0.05818438027181827 2404 72-73 -0.059743114988620505 2404 74-75 0.2732251406864279 2404 76-77 0.051447509668726354 2404 78-79 0.030897672698984024 2404 80-81 -0.1859983242404155 2404 82-83 -0.0011289391759987666 2404 84-85 0.07622275745883655 2404 86-87 0.15816919290108444 2404 88-89 0.3141140917929235 2404 90-91 -0.8272459416294566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 16.0 31 396.0 32 2939.0 33 5414.0 34 7132.0 35 9012.0 36 13125.0 37 20487.0 38 54480.0 39 333897.0 40 341729.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.63592939049148 13.704970334217156 29.251260748219355 30.407839527072007 2 25.61590472180939 19.601545024321705 28.829210394168875 25.953339859700026 3 25.1211925627139 20.08975160438284 23.14495809774277 31.644097735160486 4 25.830589112470154 21.94053716142105 21.468831272578797 30.760042453529994 5 26.290755959407935 22.226477155867094 21.26290375551433 30.21986312921064 6 26.33136619893334 25.283848110511194 24.10077780849224 24.284007882063232 7 24.31631176715989 25.113393277176666 24.31504374057698 26.255251215086474 8 23.370617541626142 26.476902261779017 24.975812392931005 25.17666780366384 9 23.156574654431054 26.579358809678087 25.378410833004704 24.885655702886154 10-11 22.940629727361607 25.533236878777927 25.45518984259986 26.070943551260612 12-13 23.18529545653395 25.226754853688753 25.25274939863839 26.335200291138904 14-15 23.673929500258044 24.756126787441975 25.248755114902227 26.321188597397754 16-17 23.624286259537143 24.620067535095806 25.032683385174487 26.722962820192564 18-19 23.655606516135006 24.978348446096824 24.850848373185293 26.515196664582874 20-21 23.85145322186534 25.166333387013122 24.58671843596529 26.39549495515624 22-23 23.65148542974055 24.75397114225103 24.866762106800806 26.727781321207615 24-25 23.724650563574414 25.02577264029763 24.85427204495915 26.3953047511688 26-27 23.93019767266401 25.14205067795041 24.682391041645797 26.24536060773978 28-29 23.991379955289382 24.764876170864046 24.68949199051009 26.554251883336484 30-31 24.16982194348145 24.938564034155608 24.56842660526029 26.323187417102655 32-33 24.18724646602783 24.94271033214046 24.57744608537378 26.292597116457934 34-35 24.14974020634097 24.59597560409344 24.708089640084335 26.546194549481257 36-37 24.180270997999823 24.908085787532748 24.62894411139316 26.282699103074275 38-39 24.438769371627114 24.864057470016345 24.53729652638591 26.15987663197063 40-41 24.319457480178823 24.565061007236892 24.65482747548596 26.46065403709833 42-43 24.45868653844426 24.819357995006463 24.618277124069838 26.103678342479437 44-45 24.579401152425632 24.795578746621956 24.450233518740173 26.174786582212246 46-47 24.454260141810995 24.60360339416483 24.55403928862025 26.388097175403928 48-49 24.60400538000272 24.68066241283515 24.58028773407415 26.135044473087977 50-51 24.838652409334667 24.641311478249197 24.37155086373192 26.14848524868421 52-53 24.802322431722626 24.457961865953763 24.429225895349248 26.310489806974363 54-55 24.968069297679207 24.637742798998325 24.42766390986456 25.966523993457912 56-57 25.072296658114457 24.58894793647822 24.27097028568349 26.067785119723837 58-59 24.93450589995953 24.36741327326178 24.323959702696005 26.374121124082688 60-61 25.270993839025557 24.46949104310587 24.1404052730539 26.119109844814677 62-63 25.280723094192325 24.47600311327876 24.07295867083335 26.170315121695566 64-65 25.25005301477345 24.315225842934897 24.0848324733159 26.34988866897576 66-67 25.485418621807582 24.539925022959835 23.81625104762096 26.158405307611627 68-69 25.749519566360167 24.32732109053409 23.786707571749066 26.13645177135668 70-71 25.609311028977526 23.999522239106057 23.87046044206154 26.52070628985488 72-73 25.81311846635853 24.057759717095266 23.755312218878927 26.373809597667275 74-75 25.885721102230708 24.291377237816462 23.668321419723064 26.15458024022977 76-77 26.04665992347949 24.027002066933463 23.586679273494877 26.339658736092176 78-79 26.25658674415646 23.826103258722622 23.302511499601614 26.6147984975193 80-81 26.606546426185705 23.866065464261858 23.028557114228455 26.49883099532398 82-83 27.00530444045147 23.52567758040047 22.622954347381672 26.846063631766388 84-85 27.804630969609264 23.19247467438495 21.68740955137482 27.31548480463097 86-87 28.388973191704604 21.684791772045187 21.606811667509696 28.319423368740516 88-89 27.69610435395734 21.233915036136082 21.769786708972326 29.30019390093425 90-91 25.258689307508625 22.30476695852127 24.6749801008225 27.761563633147606 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 1.5 12 4.0 13 6.0 14 8.0 15 11.0 16 17.0 17 28.5 18 45.0 19 74.5 20 123.0 21 180.5 22 258.0 23 391.5 24 581.0 25 801.0 26 1136.5 27 1598.0 28 2157.5 29 2895.0 30 3890.5 31 5164.0 32 6753.0 33 8547.5 34 10882.0 35 13816.0 36 17248.5 37 20798.5 38 24803.5 39 29645.0 40 34482.0 41 39564.5 42 44794.0 43 49909.5 44 54720.5 45 58712.0 46 62252.5 47 65044.0 48 66408.0 49 67042.5 50 67178.5 51 66122.5 52 63939.5 53 61109.0 54 57666.0 55 54039.0 56 50397.5 57 46661.5 58 43050.5 59 39527.0 60 36177.5 61 32981.0 62 30193.5 63 27505.5 64 24588.0 65 22102.5 66 19715.0 67 17444.0 68 15234.5 69 12876.0 70 10784.0 71 9060.5 72 7541.0 73 5963.5 74 4574.0 75 3591.5 76 2753.5 77 1943.5 78 1436.5 79 1100.5 80 762.5 81 505.5 82 351.5 83 242.5 84 167.5 85 114.5 86 73.0 87 47.5 88 31.5 89 17.5 90 7.5 91 3.5 92 3.0 93 3.0 94 3.0 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0010144212663274273 2 0.003930882407018781 3 7.608159497455705E-4 4 0.0 5 0.0 6 1.268026582909284E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 12120.0 32-33 26440.0 34-35 28852.0 36-37 31525.0 38-39 33211.0 40-41 35118.0 42-43 36702.0 44-45 37530.0 46-47 37858.0 48-49 37957.0 50-51 37499.0 52-53 36836.0 54-55 35727.0 56-57 34314.0 58-59 32622.0 60-61 31057.0 62-63 29943.0 64-65 27534.0 66-67 25645.0 68-69 23726.0 70-71 22238.0 72-73 20483.0 74-75 18474.0 76-77 16655.0 78-79 15158.0 80-81 13835.0 82-83 12123.0 84-85 11194.0 86-87 9841.0 88-89 8749.0 90-91 7661.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.90981484231801 #Duplication Level Percentage of deduplicated Percentage of total 1 99.9123915214827 99.82228537364522 2 0.08495039230889613 0.1697475593272818 3 0.0026580862084071523 0.007967067027506332 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0012680265829092842 0.0 0.0 0.0 0.0 2 0.0012680265829092842 0.0 0.0 0.0 0.0 3 0.0012680265829092842 0.0 0.0 0.0 1.268026582909284E-4 4 0.0012680265829092842 0.0 0.0 0.0 1.268026582909284E-4 5 0.0013948292412002126 0.0 0.0 0.0 1.268026582909284E-4 6 0.0017752372160729979 0.0 0.0 0.0 1.268026582909284E-4 7 0.0020288425326548546 0.0 0.0 0.0 1.268026582909284E-4 8 0.009890607346692415 0.0 0.0 0.0 1.268026582909284E-4 9 0.010271015321565201 0.0 0.0 0.0 1.268026582909284E-4 10-11 0.010271015321565201 0.0 0.0 0.0 2.536053165818568E-4 12-13 0.010271015321565201 0.0 6.34013291454642E-5 0.0 2.536053165818568E-4 14-15 0.010271015321565201 0.0 1.268026582909284E-4 0.0 2.536053165818568E-4 16-17 0.01039781797985613 0.0 1.268026582909284E-4 0.0 3.8040797487278523E-4 18-19 0.01039781797985613 0.0 1.268026582909284E-4 0.0 3.8040797487278523E-4 20-21 0.01039781797985613 0.0 1.268026582909284E-4 0.0 3.8040797487278523E-4 22-23 0.01039781797985613 0.0 1.268026582909284E-4 0.0 4.4380930401824946E-4 24-25 0.010714824625583451 0.0 1.268026582909284E-4 0.0 6.340132914546421E-4 26-27 0.011222035258747164 0.0 1.268026582909284E-4 0.0 6.340132914546421E-4 28-29 0.011412239246183557 0.0 1.268026582909284E-4 0.0 6.974146206001063E-4 30-31 0.011539041904474485 0.0 1.268026582909284E-4 0.0 7.608159497455705E-4 32-33 0.011539041904474485 6.34013291454642E-5 1.268026582909284E-4 0.0 7.608159497455705E-4 34-35 0.011539041904474485 1.268026582909284E-4 1.268026582909284E-4 0.0 7.608159497455705E-4 36-37 0.011665844562765412 1.268026582909284E-4 1.268026582909284E-4 0.0 8.876186080364989E-4 38-39 0.011982851208492734 1.268026582909284E-4 1.268026582909284E-4 0.0 8.876186080364989E-4 40-41 0.012173055195929127 1.268026582909284E-4 1.268026582909284E-4 0.0 8.876186080364989E-4 42-43 0.012426660512510984 1.268026582909284E-4 1.268026582909284E-4 0.0 8.876186080364989E-4 44-45 0.012426660512510984 1.268026582909284E-4 1.268026582909284E-4 0.0 9.510199371819631E-4 46-47 0.012553463170801912 1.268026582909284E-4 1.268026582909284E-4 0.0 0.0010144212663274273 48-49 0.01280706848738377 1.268026582909284E-4 1.268026582909284E-4 0.0 0.0010778225954728915 50-51 0.012933871145674698 1.9020398743639262E-4 1.268026582909284E-4 0.0 0.0011412239246183556 52-53 0.013060673803965625 2.536053165818568E-4 1.268026582909284E-4 0.0 0.0011412239246183556 54-55 0.013187476462256554 2.536053165818568E-4 1.9020398743639262E-4 0.0 0.0011412239246183556 56-57 0.013250877791402018 2.536053165818568E-4 2.536053165818568E-4 0.0 0.0011412239246183556 58-59 0.013504483107983876 2.536053165818568E-4 2.536053165818568E-4 0.0 0.0011412239246183556 60-61 0.01356788443712934 2.536053165818568E-4 2.536053165818568E-4 0.0 0.0011412239246183556 62-63 0.01388489108285666 2.536053165818568E-4 2.536053165818568E-4 0.0 0.0011412239246183556 64-65 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