##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-3C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 637934 Sequences flagged as poor quality 0 Sequence length 31-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.26839767123245 40.0 40.0 40.0 40.0 40.0 2 39.142243554975906 40.0 40.0 40.0 40.0 40.0 3 39.1198211727232 40.0 40.0 40.0 40.0 40.0 4 39.047061294742086 40.0 40.0 40.0 40.0 40.0 5 39.17954521941141 40.0 40.0 40.0 40.0 40.0 6 39.24207206388122 40.0 40.0 40.0 40.0 40.0 7 39.292654099013376 40.0 40.0 40.0 40.0 40.0 8 39.30811651362053 40.0 40.0 40.0 40.0 40.0 9 39.323045957732305 40.0 40.0 40.0 40.0 40.0 10-11 39.31409518853047 40.0 40.0 40.0 40.0 40.0 12-13 39.3173619841551 40.0 40.0 40.0 40.0 40.0 14-15 39.325895782322306 40.0 40.0 40.0 40.0 40.0 16-17 39.463101198556586 40.0 40.0 40.0 40.0 40.0 18-19 39.47397379666235 40.0 40.0 40.0 40.0 40.0 20-21 39.49313565353155 40.0 40.0 40.0 40.0 40.0 22-23 39.49117933830145 40.0 40.0 40.0 40.0 40.0 24-25 39.491533606924854 40.0 40.0 40.0 40.0 40.0 26-27 39.48282424200623 40.0 40.0 40.0 40.0 40.0 28-29 39.485435797433595 40.0 40.0 40.0 40.0 40.0 30-31 39.44886461608881 40.0 40.0 40.0 40.0 40.0 32-33 39.442915166347504 40.0 40.0 40.0 40.0 40.0 34-35 39.43868403380073 40.0 40.0 40.0 40.0 40.0 36-37 39.42551841213471 40.0 40.0 40.0 40.0 40.0 38-39 39.40333046123284 40.0 40.0 40.0 40.0 40.0 40-41 39.391957297014315 40.0 40.0 40.0 40.0 40.0 42-43 39.38406996046611 40.0 40.0 40.0 40.0 40.0 44-45 39.36688822749045 40.0 40.0 40.0 40.0 40.0 46-47 39.349503942619556 40.0 40.0 40.0 40.0 40.0 48-49 39.33584091470168 40.0 40.0 40.0 40.0 40.0 50-51 39.32510794317564 40.0 40.0 40.0 40.0 40.0 52-53 39.31051604775913 40.0 40.0 40.0 40.0 40.0 54-55 39.27797088831477 40.0 40.0 40.0 40.0 40.0 56-57 39.26691282494051 40.0 40.0 40.0 40.0 40.0 58-59 39.24732797275288 40.0 40.0 40.0 40.0 40.0 60-61 39.22303207549156 40.0 40.0 40.0 40.0 40.0 62-63 39.36312701862586 40.0 40.0 40.0 40.0 40.0 64-65 39.35261804603522 40.0 40.0 40.0 40.0 40.0 66-67 39.36311458984884 40.0 40.0 40.0 40.0 40.0 68-69 39.34689602047297 40.0 40.0 40.0 40.0 40.0 70-71 39.355028628617895 40.0 40.0 40.0 40.0 40.0 72-73 39.33222181680207 40.0 40.0 40.0 40.0 40.0 74-75 39.3574019412784 40.0 40.0 40.0 40.0 40.0 76-77 39.32861251331954 40.0 40.0 40.0 40.0 40.0 78-79 39.32047816486036 40.0 40.0 40.0 40.0 40.0 80-81 39.3107374149872 40.0 40.0 40.0 40.0 40.0 82-83 39.31670924222374 40.0 40.0 40.0 40.0 40.0 84-85 39.30802280404824 40.0 40.0 40.0 40.0 40.0 86-87 39.31151741078982 40.0 40.0 40.0 40.0 40.0 88-89 39.36860978871762 40.0 40.0 40.0 40.0 40.0 90-91 39.47038290977642 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1488071491389178 1101 2 0.2305040267307774 1101 3 0.19328026750518745 1101 4 0.07986802231778256 1101 5 0.007249905006609936 1101 6 -0.02234012385188322 1101 7 0.030459904893774592 1101 8 0.019411200459835243 1101 9 -0.03441974490217348 1101 10-11 -0.014268250414254169 1101 12-13 0.13282210739941291 1101 14-15 -0.05456115435833908 1101 16-17 -0.031391022398665314 1101 18-19 0.050199771601000975 1101 20-21 0.05158258988895881 1101 22-23 0.01675605289704407 1101 24-25 0.09915871685980449 1101 26-27 0.020071318212444567 1101 28-29 0.001414068998982998 1101 30-31 0.039735224545822234 1101 32-33 0.05552759829285492 1101 34-35 0.007473241061710212 1101 36-37 0.04277749827288346 1101 38-39 0.06073438392315467 1101 40-41 0.017817628113292017 1101 42-43 0.0889080760697496 1101 44-45 0.062494390321397475 1101 46-47 0.12817245726191828 1101 48-49 0.05699481083733815 1101 50-51 0.07680887083485999 1101 52-53 0.14517626696039798 1101 54-55 0.0973382378638874 1101 56-57 0.048801933343824544 1101 58-59 0.12947045317264383 1101 60-61 0.06150138142314887 1101 62-63 -0.13347215847577587 1101 64-65 -0.08038875642316157 1101 66-67 0.10314115830558279 1101 68-69 0.07249893861926182 1101 70-71 0.08345965413154488 1101 72-73 0.08821689188447124 1101 74-75 -0.011891413641357929 1101 76-77 0.01443004731142139 1101 78-79 0.2112054816082889 1101 80-81 0.3256548529882153 1101 82-83 -0.14942382746052374 1101 84-85 0.13919299173456068 1101 86-87 -0.05174174842647261 1101 88-89 0.33380993575335083 1101 90-91 0.5785425768701415 1102 1 0.3284650874602235 1102 2 0.17033949379396773 1102 3 0.2707322434720183 1102 4 0.12311685269532546 1102 5 0.08646387035918934 1102 6 -0.0020666679348622097 1102 7 0.02775798635027371 1102 8 0.09930517886866852 1102 9 0.10484692901796677 1102 10-11 0.05005191786630547 1102 12-13 -0.036659813320135015 1102 14-15 -0.07041150312947764 1102 16-17 0.08375332170606953 1102 18-19 0.02493653099122639 1102 20-21 0.018793871456679767 1102 22-23 0.04422858036956967 1102 24-25 -0.0050132387972965375 1102 26-27 0.019743400805310785 1102 28-29 -0.13210271674552843 1102 30-31 0.053942964001088 1102 32-33 0.051449192231125096 1102 34-35 0.05836685736230862 1102 36-37 -0.07209075712223978 1102 38-39 0.09321653346786718 1102 40-41 -0.038243535197835854 1102 42-43 0.017123419346766866 1102 44-45 -0.01730597183114213 1102 46-47 -0.08180972869372027 1102 48-49 0.11271415619672354 1102 50-51 -0.09438193481501855 1102 52-53 0.023563604053862264 1102 54-55 -0.07584108043747051 1102 56-57 -0.046702703398807444 1102 58-59 0.14211745952417232 1102 60-61 0.015230005247289569 1102 62-63 -0.10774297180027759 1102 64-65 -0.11297844144284142 1102 66-67 0.0544777466126547 1102 68-69 -0.08222385140310706 1102 70-71 0.13012061948992226 1102 72-73 0.008106266572553977 1102 74-75 0.1026281542431633 1102 76-77 0.14817411098400157 1102 78-79 0.2283147544904196 1102 80-81 0.32335786583805515 1102 82-83 0.07818499538853985 1102 84-85 -0.05469857724665417 1102 86-87 -0.5955689089203062 1102 88-89 0.563597169795905 1102 90-91 -0.6309354451078804 1103 1 0.36753712653226245 1103 2 0.24604156949604317 1103 3 0.116886089625865 1103 4 0.33557106514953716 1103 5 9.937848891041767E-4 1103 6 0.07363540776721322 1103 7 0.11567007426236131 1103 8 0.09197917154266122 1103 9 0.08042690459794244 1103 10-11 0.07569294350733458 1103 12-13 0.09398731732699872 1103 14-15 0.04680461408663916 1103 16-17 0.10084733880009367 1103 18-19 0.03226253831723369 1103 20-21 0.065191917854726 1103 22-23 -0.04734651120551803 1103 24-25 -0.04896928275334034 1103 26-27 0.04660542766734466 1103 28-29 0.00587042122761261 1103 30-31 -0.0266431165849923 1103 32-33 -0.014390550600325014 1103 34-35 0.0380414510147844 1103 36-37 -0.03551921713089712 1103 38-39 0.029978223264535586 1103 40-41 0.048947631729866714 1103 42-43 -0.1046789091107172 1103 44-45 -0.05451908834322694 1103 46-47 0.03640984711551454 1103 48-49 -0.04005050717999126 1103 50-51 -0.00878475871968476 1103 52-53 -0.11962125767035303 1103 54-55 -0.060876402143492214 1103 56-57 -0.05534191569934421 1103 58-59 0.20420436393924035 1103 60-61 -0.01837634884615369 1103 62-63 -0.08988081326290853 1103 64-65 0.07037302983239613 1103 66-67 -0.1077696281328997 1103 68-69 -0.007081449584809718 1103 70-71 -0.03986280639063722 1103 72-73 -0.3546329638460719 1103 74-75 -0.16625380980024573 1103 76-77 -0.025798598072526602 1103 78-79 0.1336683946234345 1103 80-81 0.0674539025155454 1103 82-83 -0.19769892279444434 1103 84-85 0.2882590128161695 1103 86-87 -0.6359729493243478 1103 88-89 -0.3221441531993037 1103 90-91 -0.7519047535771932 1104 1 0.3318289243527843 1104 2 0.24048336581356722 1104 3 0.18438040331009375 1104 4 0.1461512625996022 1104 5 0.21629079737251544 1104 6 0.07050632374715349 1104 7 -0.030669713343549176 1104 8 0.11602130109475439 1104 9 0.07457239698493368 1104 10-11 0.0014537789985311633 1104 12-13 -0.021526841563485277 1104 14-15 -0.10402983217571204 1104 16-17 -0.03750369404097853 1104 18-19 -0.059155097062884465 1104 20-21 0.010611874998012638 1104 22-23 0.024376217023657887 1104 24-25 0.03252145524384531 1104 26-27 0.01707809685044026 1104 28-29 -0.08872353503106467 1104 30-31 0.04918062981320048 1104 32-33 0.05843159235070061 1104 34-35 0.0028264031481697316 1104 36-37 0.14417563616381557 1104 38-39 0.025578362042679714 1104 40-41 -0.011533635876809 1104 42-43 0.030708488473855766 1104 44-45 0.09039751383343031 1104 46-47 -0.0013053831735874155 1104 48-49 0.05149763333592006 1104 50-51 0.05407524740101621 1104 52-53 0.0688097618539274 1104 54-55 0.1273212546154241 1104 56-57 0.0023784860747682046 1104 58-59 0.05751042612683932 1104 60-61 0.18440790749072278 1104 62-63 0.2056314594841666 1104 64-65 0.10986232835298892 1104 66-67 0.13458021268130693 1104 68-69 0.12990257715056686 1104 70-71 0.16752632478139873 1104 72-73 0.05160623202907999 1104 74-75 -0.018657044294791092 1104 76-77 0.11244329599231406 1104 78-79 -0.0785119627166111 1104 80-81 0.258083173001431 1104 82-83 -0.37383518569377827 1104 84-85 0.06728510164485613 1104 86-87 -0.4454552402644296 1104 88-89 -0.7810148620903163 1104 90-91 -1.4338200604924936 1201 1 0.27583774141969997 1201 2 0.08260872821888654 1201 3 0.11692703337938326 1201 4 0.1363036658821386 1201 5 0.07645604717770738 1201 6 0.12031713588606152 1201 7 0.08448848140412224 1201 8 -0.23179233306716895 1201 9 0.08028945056827297 1201 10-11 -0.003673598134660949 1201 12-13 0.013306188741672997 1201 14-15 0.0396687027591085 1201 16-17 -0.026035891077157203 1201 18-19 0.07179519461635664 1201 20-21 -0.0039489213459944494 1201 22-23 0.05567820858566819 1201 24-25 -0.10384122385282524 1201 26-27 -0.03254177243794487 1201 28-29 0.10065667290008662 1201 30-31 -0.06944542405796739 1201 32-33 0.013929782693118398 1201 34-35 -0.06791935161349727 1201 36-37 -0.08759205931815472 1201 38-39 -0.016904637888742968 1201 40-41 -0.1283425357560617 1201 42-43 0.029812630112850513 1201 44-45 0.03148587100542244 1201 46-47 -0.04116724995056842 1201 48-49 0.009906767217508161 1201 50-51 0.013922730072060574 1201 52-53 -0.010062254424838102 1201 54-55 -0.01855555402548248 1201 56-57 0.010733019802195543 1201 58-59 0.09685300186809798 1201 60-61 0.0286322221246067 1201 62-63 -0.32228370980409693 1201 64-65 -0.18695749587868704 1201 66-67 -0.32598797590883066 1201 68-69 -0.25882069380654116 1201 70-71 -0.4055887824059212 1201 72-73 -0.22722216162341624 1201 74-75 -0.3706756319678135 1201 76-77 -0.38893265604355065 1201 78-79 -0.26311877052518895 1201 80-81 -0.5182820705737825 1201 82-83 -0.42450189696583607 1201 84-85 -0.752863773990299 1201 86-87 -0.02982459317598085 1201 88-89 -0.0848013065706894 1201 90-91 0.043744042071608646 1202 1 0.10205166761186746 1202 2 0.10566507741966547 1202 3 0.15038274797598206 1202 4 0.31731187221273416 1202 5 -0.1682344077857536 1202 6 0.0634723260918193 1202 7 0.04201492909490412 1202 8 0.05505998808185808 1202 9 0.027679079282705743 1202 10-11 0.027245103734756526 1202 12-13 0.07343212678352273 1202 14-15 -0.02431119208192456 1202 16-17 -0.0058507045919782286 1202 18-19 0.00649554772023464 1202 20-21 0.037575532676790147 1202 22-23 0.003274224057655317 1202 24-25 0.05127878232473648 1202 26-27 6.654356546675899E-4 1202 28-29 0.03788842276495075 1202 30-31 0.028078636847190808 1202 32-33 0.002191722115632899 1202 34-35 0.05121128850841217 1202 36-37 0.019806703406629822 1202 38-39 -0.03740798223621766 1202 40-41 -0.035598574163984154 1202 42-43 -0.015362130430396803 1202 44-45 0.08989056080573476 1202 46-47 0.07100420852113842 1202 48-49 0.07004435756213212 1202 50-51 0.056465669815182196 1202 52-53 0.06281502500894476 1202 54-55 0.05418810001104646 1202 56-57 0.08800166577928792 1202 58-59 0.06133414324359876 1202 60-61 -0.05037102066544463 1202 62-63 -0.05821777214225676 1202 64-65 -0.03534233813560661 1202 66-67 -0.15901634107962082 1202 68-69 -0.2842531120942411 1202 70-71 -0.12973679150428552 1202 72-73 -0.16783565029242453 1202 74-75 -0.036175214448192605 1202 76-77 -0.3059487015922002 1202 78-79 -0.18188961666900383 1202 80-81 -0.32750854674183216 1202 82-83 -0.07217166785542162 1202 84-85 -0.2562607271234114 1202 86-87 0.12554220219080747 1202 88-89 0.24993896801141346 1202 90-91 0.29282829115585685 1203 1 0.27530391171840307 1203 2 0.28822447619507585 1203 3 0.20978530510572568 1203 4 0.277066129225247 1203 5 -0.009279812481267413 1203 6 0.1709817889845624 1203 7 0.011107969700255182 1203 8 0.12037371606623282 1203 9 0.022593698377640692 1203 10-11 0.03507119965978234 1203 12-13 0.0941813884888063 1203 14-15 0.07967783405663198 1203 16-17 0.15101294380440322 1203 18-19 0.08829825886908793 1203 20-21 0.0994630230935698 1203 22-23 0.12614021711991796 1203 24-25 0.14953472220227582 1203 26-27 0.033193033609784095 1203 28-29 0.13614229988658622 1203 30-31 0.06061586099656324 1203 32-33 0.06353831908246832 1203 34-35 0.012163382546148682 1203 36-37 0.03492089688533184 1203 38-39 0.017806365801320112 1203 40-41 0.06880432964312888 1203 42-43 0.06821752943776005 1203 44-45 -0.03144078620969282 1203 46-47 6.589214092045381E-4 1203 48-49 0.12136610971942474 1203 50-51 0.019362965799352594 1203 52-53 0.0327534077969176 1203 54-55 -0.050781893357672914 1203 56-57 0.12289806082389276 1203 58-59 2.8540975819879577E-4 1203 60-61 0.011369681813071963 1203 62-63 0.18867870528589492 1203 64-65 -0.007609910422473831 1203 66-67 0.039555319259498845 1203 68-69 0.058778143637930214 1203 70-71 0.012519097260103251 1203 72-73 0.11174133158107935 1203 74-75 0.10651849959372583 1203 76-77 -0.11160111128116057 1203 78-79 -0.1038928932366261 1203 80-81 0.07173083978724293 1203 82-83 0.2818791499911768 1203 84-85 0.23055888601199115 1203 86-87 0.5020127904261003 1203 88-89 0.40727932350845464 1203 90-91 0.6356854340129985 1204 1 0.17560180853702434 1204 2 0.0844000579393267 1204 3 -0.17563515710680377 1204 4 -0.09252618148926217 1204 5 -0.149075478803681 1204 6 -0.08357626442467847 1204 7 -0.1434734579167838 1204 8 -0.1686110641747689 1204 9 -0.036624939983582294 1204 10-11 -0.19358027432439684 1204 12-13 -0.2300059433536603 1204 14-15 -0.1497952184657123 1204 16-17 -0.1423734815829647 1204 18-19 -0.1873078542322446 1204 20-21 -0.07075956235905068 1204 22-23 -0.17176853726689245 1204 24-25 -0.13196699605066442 1204 26-27 -0.12706318749364698 1204 28-29 -0.1938471402762758 1204 30-31 -0.13867429249406626 1204 32-33 -0.297073079931117 1204 34-35 -0.1149977330400489 1204 36-37 -0.10791909875500494 1204 38-39 -0.17264430595183455 1204 40-41 -0.1542571970596427 1204 42-43 -0.07965856532954518 1204 44-45 -0.08725900386951224 1204 46-47 -0.09345456596621204 1204 48-49 -0.04776819774428276 1204 50-51 -0.10482159414005565 1204 52-53 -0.15026060437023858 1204 54-55 -0.19050283453364614 1204 56-57 -0.23589779229066465 1204 58-59 -0.11226826345695429 1204 60-61 -0.2731215701045855 1204 62-63 -0.23983239447984772 1204 64-65 -0.23573150878422666 1204 66-67 -0.208215355456133 1204 68-69 -0.2093644549634277 1204 70-71 -0.31519124706874635 1204 72-73 -0.009415434749215024 1204 74-75 -0.05194278121390994 1204 76-77 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0.07320483495556829 2401 50-51 0.039398586874689556 2401 52-53 0.046211176719864966 2401 54-55 0.1753362233083493 2401 56-57 0.11678415689900845 2401 58-59 0.08946133685133617 2401 60-61 -0.08100897657127604 2401 62-63 -0.00872804590680687 2401 64-65 0.14904146244452932 2401 66-67 0.04678702931698098 2401 68-69 0.1585580132874469 2401 70-71 0.13274600849110385 2401 72-73 -0.0875711880943868 2401 74-75 0.11375575463151932 2401 76-77 0.03299713274815019 2401 78-79 0.09218062842958119 2401 80-81 0.07823130484337781 2401 82-83 0.2504968951542992 2401 84-85 0.08918109697009413 2401 86-87 0.0656930317534048 2401 88-89 -0.21761863567522255 2401 90-91 0.07259019591776195 2402 1 0.28480757681004576 2402 2 0.2265682419542543 2402 3 0.19420621584574604 2402 4 0.26963699921547146 2402 5 0.21271360541087603 2402 6 0.2333507264067336 2402 7 0.17603350870892598 2402 8 0.1539964805037357 2402 9 0.08374659495919445 2402 10-11 0.1156993918267446 2402 12-13 0.08061899589021237 2402 14-15 0.20740657664458695 2402 16-17 0.06322617159456456 2402 18-19 -0.009099251210813009 2402 20-21 0.10049646660670675 2402 22-23 -0.012176302441183395 2402 24-25 -0.027665946046582235 2402 26-27 0.12790798818750204 2402 28-29 0.07982010642423631 2402 30-31 0.13564854448417663 2402 32-33 0.026032619641682686 2402 34-35 0.04265376586236158 2402 36-37 0.023431708749683366 2402 38-39 0.13568357200702508 2402 40-41 0.0719477593373341 2402 42-43 -0.02647181084154937 2402 44-45 0.09713317715733893 2402 46-47 0.043473464777605386 2402 48-49 0.07885069428505886 2402 50-51 0.11451524350271569 2402 52-53 0.033860084829754555 2402 54-55 0.11935006758078259 2402 56-57 0.15439479455686467 2402 58-59 0.0971240353057965 2402 60-61 0.25532687932302167 2402 62-63 0.08090806591859945 2402 64-65 0.24475646803473694 2402 66-67 0.25547989257262316 2402 68-69 0.09173849201601314 2402 70-71 0.173258728772133 2402 72-73 0.1322272894370755 2402 74-75 0.17322126135579197 2402 76-77 0.20277598373957062 2402 78-79 0.3960894401222532 2402 80-81 0.028144025493801905 2402 82-83 0.14611418572454937 2402 84-85 0.415959094253715 2402 86-87 0.3970919972500795 2402 88-89 0.03130097517987451 2402 90-91 -0.38574313741557376 2403 1 -0.3523705170734033 2403 2 -0.13752041196662645 2403 3 -0.23392427168106877 2403 4 -0.07136690335484275 2403 5 0.027745394200259454 2403 6 0.22370567572201594 2403 7 0.04237746725961955 2403 8 0.02983282195182113 2403 9 -0.028431984236725327 2403 10-11 0.07656543933340032 2403 12-13 0.10167366049980586 2403 14-15 0.13422968172971395 2403 16-17 0.08785405208859487 2403 18-19 0.1008988766346377 2403 20-21 0.051095934441015345 2403 22-23 0.08881903230761878 2403 24-25 -0.011231127130649554 2403 26-27 0.07038019952446462 2403 28-29 0.07013491464026345 2403 30-31 0.044239035847418506 2403 32-33 0.11684902899219907 2403 34-35 0.0020976931779230767 2403 36-37 0.10084087291089361 2403 38-39 0.01910122920831725 2403 40-41 0.05899753381136463 2403 42-43 0.00818670275233302 2403 44-45 0.17607735069599784 2403 46-47 0.19567561238483222 2403 48-49 0.1225294092693403 2403 50-51 0.00711149137730871 2403 52-53 0.09854284236153887 2403 54-55 0.09026817937966314 2403 56-57 0.1490804847357694 2403 58-59 -0.12691464259592777 2403 60-61 0.08574167457761206 2403 62-63 0.15553181886224365 2403 64-65 0.17479492651976614 2403 66-67 0.04902881701124784 2403 68-69 0.18603903852651626 2403 70-71 0.11786016322205484 2403 72-73 0.22509413388688415 2403 74-75 0.10369638005978032 2403 76-77 0.26382128496840096 2403 78-79 -0.024992361733588098 2403 80-81 0.08886196544315084 2403 82-83 0.01889513695314804 2403 84-85 0.36924880607843136 2403 86-87 0.1690442958337499 2403 88-89 0.4004766024200208 2403 90-91 0.28530949416337137 2404 1 -0.4540583312461024 2404 2 -0.1950879658680833 2404 3 -0.3906193053546829 2404 4 -0.3217480239786994 2404 5 -0.2482448492401872 2404 6 -0.20715991795853483 2404 7 -0.09979027718208755 2404 8 0.10350367531368221 2404 9 4.278683807967809E-5 2404 10-11 0.04279727721206683 2404 12-13 0.06055324532433559 2404 14-15 0.04616862537907451 2404 16-17 -0.04604517668089869 2404 18-19 -0.047643859680619016 2404 20-21 -0.031689067296632345 2404 22-23 0.018147702996451187 2404 24-25 0.07003051576931796 2404 26-27 0.059202373465083724 2404 28-29 5.15007919908328E-4 2404 30-31 0.04793651187137726 2404 32-33 0.09487999156938542 2404 34-35 0.09469964366444827 2404 36-37 0.019973670882762917 2404 38-39 0.11554360751794945 2404 40-41 0.05273102268512986 2404 42-43 0.038287184405284336 2404 44-45 5.812843934904777E-4 2404 46-47 0.07986360023458872 2404 48-49 0.09714316967343706 2404 50-51 0.014076020036604575 2404 52-53 0.032954419932053725 2404 54-55 0.07917239496296702 2404 56-57 0.07361557586744283 2404 58-59 0.013029109387780125 2404 60-61 -0.010306828224038611 2404 62-63 0.10376810046130203 2404 64-65 0.19543717184647846 2404 66-67 0.1875099947928618 2404 68-69 0.1166937813479052 2404 70-71 0.062376500506388766 2404 72-73 -0.039444874241247874 2404 74-75 0.11750073333716671 2404 76-77 0.038403642607399036 2404 78-79 -0.0362795807845373 2404 80-81 0.1593881647650548 2404 82-83 0.3233911970485508 2404 84-85 0.27784647346487645 2404 86-87 -0.19656959905142912 2404 88-89 -0.19609771283770527 2404 90-91 -0.49553149720392753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 11.0 31 344.0 32 2655.0 33 4677.0 34 6248.0 35 7810.0 36 11376.0 37 17721.0 38 50330.0 39 300632.0 40 236130.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.720116691199408 14.011095382985095 26.21789777136112 32.05089015445438 2 25.38188522123006 19.0633778970603 27.400421390588942 28.154315491120695 3 25.83394991284283 19.637012327409987 21.690222094029423 32.83881566571776 4 26.83866983104835 21.626375142256098 19.985923308680835 31.549031718014714 5 27.120987437571912 21.570883508325313 20.208673624544232 31.099455429558542 6 27.253289525248693 24.259406145463323 23.196757031291636 25.290547297996348 7 25.14586148410337 24.197957782466524 23.380945364253982 27.27523536917612 8 23.86579175902209 25.513924637971954 24.318816680095434 26.30146692291052 9 23.918305028419866 25.341806519169698 24.63593412484677 26.103954327563667 10-11 23.735527499710003 24.562650681731967 24.555831794511658 27.145990024046373 12-13 24.303846479416364 24.150303949938394 24.20485504770086 27.340994522944378 14-15 24.679433922631492 23.56615261139867 24.10633388406951 27.64807958190032 16-17 24.603485627039788 23.377888621706948 23.95937510776977 28.059250643483498 18-19 24.798254991895714 23.58472820072296 23.786786720883352 27.830230086497977 20-21 24.71007972611587 23.895732160380227 23.655660303416965 27.73852781008694 22-23 24.705220289246224 23.65448463320657 23.710916803305672 27.929378274241536 24-25 24.975937949693854 23.799640715183703 23.546401351863985 27.678019983258455 26-27 25.012540482244244 23.725416735900577 23.674392648769306 27.587650133085866 28-29 24.895600485316663 23.56301749083761 23.80575420027777 27.735627823567953 30-31 25.19719908329075 23.72243837136757 23.549458094411023 27.53090445093066 32-33 25.27669190426181 23.69972733198088 23.597018659671747 27.426562104085555 34-35 25.184252498643637 23.429809194485664 23.700509206905345 27.685429099965354 36-37 25.39493464093524 23.66217704277046 23.620115735892007 27.322772580402294 38-39 25.427984022045752 23.643199589684745 23.611556633502413 27.317259754767086 40-41 25.333132541528286 23.46171083635498 23.758660273792252 27.446496348324484 42-43 25.495231452672662 23.725144428798906 23.574840719427435 27.204783399100997 44-45 25.59904946817823 23.612119280727534 23.60385720777343 27.184974043320803 46-47 25.51552276524149 23.529885804436613 23.64487690373292 27.30971452658898 48-49 25.815097144625927 23.617490174365503 23.58620945222769 26.98120322878088 50-51 25.89931962732532 23.54394357445698 23.489892579422047 27.066844218795655 52-53 25.75933687616665 23.441432434162895 23.637946404385364 27.16128428528509 54-55 26.008739130492124 23.55146867774819 23.4339533552537 27.005838836505987 56-57 26.049993691603028 23.678178191659025 23.32689262541306 26.94493549132489 58-59 26.073147050625895 23.504332405288935 23.452723335569218 26.969797208515956 60-61 26.376507167158238 23.51480369376627 23.187071818054715 26.92161732102078 62-63 26.40413897412778 23.421888861418534 23.178109684268698 26.99586248018499 64-65 26.39127332612506 23.30943677538368 23.29005038462999 27.00923951386127 66-67 26.715558926080124 23.4192006403014 23.011937110728365 26.85330332289011 68-69 26.75838450929063 23.5328805011584 22.79688429093439 26.911850698616586 70-71 26.737501354546694 23.436853117124524 22.855023214846387 26.970622313482394 72-73 26.917094514277046 23.589999747085155 22.69436505728521 26.79854068135259 74-75 27.14519259127104 23.420438450531407 22.497078088760563 26.937290869436993 76-77 27.07228222662491 23.188257599965915 22.59730299737969 27.142157176029485 78-79 27.41267231566966 23.246743391931197 22.306816744656633 27.033767547742503 80-81 27.898256243534803 23.09923156494754 21.734766760258115 27.267745431259545 82-83 28.092028817104346 22.778681540010844 21.615926872724454 27.513362770160356 84-85 29.124707946905005 22.405191148113005 20.65134216889596 27.81875873608603 86-87 30.223546082882674 21.4841377714798 19.918158419850197 28.37415772578733 88-89 29.3670945596464 20.839798749235904 19.859406592373162 29.93370009874453 90-91 26.873194766947954 22.076230390213514 21.062468142946138 29.98810669989239 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.5 9 1.5 10 1.0 11 0.5 12 0.0 13 2.0 14 6.0 15 9.0 16 13.5 17 20.5 18 29.5 19 42.0 20 63.5 21 90.5 22 125.0 23 187.5 24 277.0 25 381.0 26 531.5 27 756.0 28 1076.0 29 1469.0 30 1956.5 31 2520.0 32 3256.0 33 4164.5 34 5348.5 35 6957.5 36 8759.0 37 10571.0 38 12806.0 39 15581.5 40 18552.5 41 21935.5 42 25380.0 43 28800.0 44 32373.5 45 35861.5 46 39225.0 47 42311.5 48 44657.5 49 46466.5 50 47828.0 51 48425.5 52 48282.0 53 47749.0 54 46880.5 55 45471.0 56 43789.5 57 41821.5 58 39566.5 59 37292.5 60 34906.5 61 32554.0 62 30517.5 63 28171.0 64 25668.5 65 23368.0 66 20866.5 67 18544.0 68 16459.5 69 14157.5 70 11890.5 71 9921.0 72 8196.5 73 6496.5 74 4931.0 75 3772.0 76 2848.0 77 2063.0 78 1540.5 79 1118.5 80 723.0 81 493.0 82 363.0 83 242.5 84 152.0 85 98.5 86 63.0 87 41.0 88 29.5 89 17.5 90 9.0 91 5.5 92 3.5 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0017243163085836465 2 0.007210777290440704 3 9.405361683183527E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.005663155510250311 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 6772.0 32-33 15096.0 34-35 17067.0 36-37 18899.0 38-39 20234.0 40-41 22182.0 42-43 23349.0 44-45 24133.0 46-47 25066.0 48-49 25603.0 50-51 26748.0 52-53 26972.0 54-55 26651.0 56-57 26119.0 58-59 26193.0 60-61 25511.0 62-63 25102.0 64-65 24147.0 66-67 23589.0 68-69 23074.0 70-71 21819.0 72-73 21161.0 74-75 20307.0 76-77 18743.0 78-79 17918.0 80-81 16960.0 82-83 15683.0 84-85 14761.0 86-87 13626.0 88-89 12635.0 90-91 11814.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.72059938365628 #Duplication Level Percentage of deduplicated Percentage of total 1 99.73642353052773 99.45775934846425 2 0.250160850183585 0.4989237984526428 3 0.010989019154716626 0.03287494730240466 4 0.001907234057800811 0.007607620936352793 5 2.739704155208541E-4 0.0013660247024564467 6 2.453956606408122E-4 0.001468260141915006 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.837801402652939E-4 0.0 0.0 0.0 0.0 2 7.837801402652939E-4 0.0 0.0 0.0 1.567560280530588E-4 3 7.837801402652939E-4 1.567560280530588E-4 0.0 0.0 1.567560280530588E-4 4 7.837801402652939E-4 1.567560280530588E-4 0.0 0.0 1.567560280530588E-4 5 9.405361683183527E-4 1.567560280530588E-4 0.0 0.0 1.567560280530588E-4 6 9.405361683183527E-4 1.567560280530588E-4 0.0 0.0 1.567560280530588E-4 7 0.0012540482244244703 1.567560280530588E-4 0.0 0.0 1.567560280530588E-4 8 0.008464825514865174 1.567560280530588E-4 0.0 0.0 1.567560280530588E-4 9 0.008464825514865174 1.567560280530588E-4 0.0 0.0 1.567560280530588E-4 10-11 0.008621581542918233 1.567560280530588E-4 0.0 0.0 1.567560280530588E-4 12-13 0.008621581542918233 1.567560280530588E-4 0.0 0.0 1.567560280530588E-4 14-15 0.008621581542918233 2.351340420795882E-4 0.0 0.0 1.567560280530588E-4 16-17 0.008621581542918233 3.135120561061176E-4 0.0 0.0 1.567560280530588E-4 18-19 0.008621581542918233 3.135120561061176E-4 0.0 0.0 1.567560280530588E-4 20-21 0.008621581542918233 4.7026808415917635E-4 0.0 0.0 1.567560280530588E-4 22-23 0.008621581542918233 7.054021262387646E-4 0.0 0.0 1.567560280530588E-4 24-25 0.008778337570971292 7.837801402652939E-4 0.0 0.0 1.567560280530588E-4 26-27 0.008778337570971292 7.837801402652939E-4 0.0 0.0 2.351340420795882E-4 28-29 0.008778337570971292 7.837801402652939E-4 0.0 0.0 3.9189007013264697E-4 30-31 0.00893509359902435 7.837801402652939E-4 0.0 0.0 4.7026808415917635E-4 32-33 0.009483739697210055 7.837801402652939E-4 0.0 0.0 4.7026808415917635E-4 34-35 0.009718873739289645 7.837801402652939E-4 0.0 0.0 4.7026808415917635E-4 36-37 0.009718873739289645 7.837801402652939E-4 0.0 0.0 4.7026808415917635E-4 38-39 0.009718873739289645 7.837801402652939E-4 0.0 0.0 6.270241122122352E-4 40-41 0.009954007781369234 7.837801402652939E-4 0.0 0.0 7.054021262387646E-4 42-43 0.010032385795395763 7.837801402652939E-4 0.0 0.0 7.837801402652939E-4 44-45 0.010189141823448822 8.621581542918233E-4 0.0 0.0 0.0011756702103979408 46-47 0.010502653879554938 9.405361683183527E-4 0.0 0.0 0.001410804252477529 48-49 0.010816165935661056 9.405361683183527E-4 0.0 0.0 0.0015675602805305879 50-51 0.010972921963714116 9.405361683183527E-4 0.0 0.0 0.0015675602805305879 52-53 0.011129677991767173 9.405361683183527E-4 0.0 0.0 0.0015675602805305879 54-55 0.01136481203384676 9.405361683183527E-4 0.0 0.0 0.0015675602805305879 56-57 0.012070214160085526 9.405361683183527E-4 0.0 0.0 0.0015675602805305879 58-59 0.012148592174112054 9.405361683183527E-4 0.0 0.0 0.0015675602805305879 60-61 0.012226970188138585 9.405361683183527E-4 0.0 0.0 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