##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-3B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 919372 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.232282471078086 40.0 40.0 40.0 40.0 40.0 2 39.10600931940498 40.0 40.0 40.0 40.0 40.0 3 39.09777761341437 40.0 40.0 40.0 40.0 40.0 4 39.04490456529022 40.0 40.0 40.0 40.0 40.0 5 39.17065997224192 40.0 40.0 40.0 40.0 40.0 6 39.249422431833906 40.0 40.0 40.0 40.0 40.0 7 39.28370670414152 40.0 40.0 40.0 40.0 40.0 8 39.30455571846869 40.0 40.0 40.0 40.0 40.0 9 39.32532641846826 40.0 40.0 40.0 40.0 40.0 10-11 39.31808886935865 40.0 40.0 40.0 40.0 40.0 12-13 39.32108874318557 40.0 40.0 40.0 40.0 40.0 14-15 39.323240211796744 40.0 40.0 40.0 40.0 40.0 16-17 39.4638818671876 40.0 40.0 40.0 40.0 40.0 18-19 39.477752204765864 40.0 40.0 40.0 40.0 40.0 20-21 39.48848344304591 40.0 40.0 40.0 40.0 40.0 22-23 39.49372615219954 40.0 40.0 40.0 40.0 40.0 24-25 39.491831380551076 40.0 40.0 40.0 40.0 40.0 26-27 39.48220741984746 40.0 40.0 40.0 40.0 40.0 28-29 39.48220089365349 40.0 40.0 40.0 40.0 40.0 30-31 39.47015702221823 40.0 40.0 40.0 40.0 40.0 32-33 39.47469027168573 40.0 40.0 40.0 40.0 40.0 34-35 39.467367757488944 40.0 40.0 40.0 40.0 40.0 36-37 39.46608076461894 40.0 40.0 40.0 40.0 40.0 38-39 39.45086873064118 40.0 40.0 40.0 40.0 40.0 40-41 39.440515400498384 40.0 40.0 40.0 40.0 40.0 42-43 39.4361074509703 40.0 40.0 40.0 40.0 40.0 44-45 39.41791555255815 40.0 40.0 40.0 40.0 40.0 46-47 39.413822546385546 40.0 40.0 40.0 40.0 40.0 48-49 39.41503060475905 40.0 40.0 40.0 40.0 40.0 50-51 39.413077616919296 40.0 40.0 40.0 40.0 40.0 52-53 39.39912857256054 40.0 40.0 40.0 40.0 40.0 54-55 39.37527655466137 40.0 40.0 40.0 40.0 40.0 56-57 39.37394883828971 40.0 40.0 40.0 40.0 40.0 58-59 39.35468311888888 40.0 40.0 40.0 40.0 40.0 60-61 39.320849950100865 40.0 40.0 40.0 40.0 40.0 62-63 39.36054806156682 40.0 40.0 40.0 40.0 40.0 64-65 39.361449741759955 40.0 40.0 40.0 40.0 40.0 66-67 39.351575952640076 40.0 40.0 40.0 40.0 40.0 68-69 39.351848372146925 40.0 40.0 40.0 40.0 40.0 70-71 39.348157822770546 40.0 40.0 40.0 40.0 40.0 72-73 39.335657454259675 40.0 40.0 40.0 40.0 40.0 74-75 39.34044968503814 40.0 40.0 40.0 40.0 40.0 76-77 39.32603027518326 40.0 40.0 40.0 40.0 40.0 78-79 39.32212983164723 40.0 40.0 40.0 40.0 40.0 80-81 39.31409467235588 40.0 40.0 40.0 40.0 40.0 82-83 39.301449042090965 40.0 40.0 40.0 40.0 40.0 84-85 39.29540101648534 40.0 40.0 40.0 40.0 40.0 86-87 39.28854755416886 40.0 40.0 40.0 40.0 40.0 88-89 39.3434028101749 40.0 40.0 40.0 40.0 40.0 90-91 39.42363604210515 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27724781905982354 1101 2 0.15278432585036938 1101 3 0.18587797140611428 1101 4 0.07310373806818404 1101 5 0.12858217339429245 1101 6 -0.01186998276516249 1101 7 -0.003662664426627771 1101 8 0.06555649483146908 1101 9 0.024982919011300453 1101 10-11 0.1042517515214243 1101 12-13 -0.07858077819380327 1101 14-15 0.00849890636343531 1101 16-17 -0.04072621983491587 1101 18-19 0.026309108502161394 1101 20-21 0.012116268800880903 1101 22-23 0.030700495138113126 1101 24-25 0.041448177719658474 1101 26-27 -0.03609422094844916 1101 28-29 0.024380041855650347 1101 30-31 0.010972220891851237 1101 32-33 0.022831063869041657 1101 34-35 0.012944398169267401 1101 36-37 0.018458507719437023 1101 38-39 0.08121101471728309 1101 40-41 0.06316481673356833 1101 42-43 0.05241395479059463 1101 44-45 0.11574482933927044 1101 46-47 0.06492317889524912 1101 48-49 -0.03303298448812342 1101 50-51 0.11468092536538421 1101 52-53 0.04462503730596268 1101 54-55 -0.00515069160230297 1101 56-57 0.08549750792600008 1101 58-59 -0.03532467361668523 1101 60-61 -0.018796259238108348 1101 62-63 -0.03707957846938115 1101 64-65 0.09360537697000382 1101 66-67 0.047853773459358706 1101 68-69 0.01573911164769015 1101 70-71 0.0704536189154652 1101 72-73 0.07892559224815443 1101 74-75 -0.028198063866270218 1101 76-77 -0.05954448267352319 1101 78-79 0.20680677840066153 1101 80-81 0.013322777263809371 1101 82-83 -0.03151399797788201 1101 84-85 -0.32127399287426783 1101 86-87 0.15789343566343206 1101 88-89 0.2902991893848892 1101 90-91 -0.13562141806105643 1102 1 0.38174746915321833 1102 2 0.2762012672860976 1102 3 0.3558515174738446 1102 4 0.21486968280775187 1102 5 0.11809655596216118 1102 6 0.10863851508494093 1102 7 0.14435031272439147 1102 8 0.028021359410168145 1102 9 -0.005375408517828362 1102 10-11 -0.041570793714143406 1102 12-13 -0.049312703408084246 1102 14-15 -0.025563633445678136 1102 16-17 0.15718195077392494 1102 18-19 0.0030349421980844227 1102 20-21 0.03529117081875199 1102 22-23 0.013003327313633406 1102 24-25 0.06667371444992654 1102 26-27 -0.009438451294535355 1102 28-29 0.06221876055224129 1102 30-31 0.027716451568814193 1102 32-33 0.05112329728324028 1102 34-35 0.009076979775301197 1102 36-37 -0.038945089822661316 1102 38-39 0.0495880847113952 1102 40-41 0.05208338315182459 1102 42-43 0.0036294980775650743 1102 44-45 -0.04129381244336372 1102 46-47 -0.034374248152637676 1102 48-49 6.482823110331992E-5 1102 50-51 -0.01954194054771108 1102 52-53 -0.03682148070614488 1102 54-55 0.0503589086467926 1102 56-57 -0.08153162256555646 1102 58-59 0.037617183270171495 1102 60-61 -0.0977614026640552 1102 62-63 0.08382327867479944 1102 64-65 0.07034742708898989 1102 66-67 -0.017280858606966376 1102 68-69 0.028979967449082267 1102 70-71 0.017648748662885794 1102 72-73 -0.003868463183700044 1102 74-75 -0.06036508623766679 1102 76-77 -0.2281352436718791 1102 78-79 -0.05845152878537618 1102 80-81 0.04568892138414071 1102 82-83 0.1351090869330278 1102 84-85 0.06412932465859456 1102 86-87 0.002503158281122353 1102 88-89 0.10810180977237138 1102 90-91 0.20756989355130884 1103 1 0.384403851871312 1103 2 0.21207872546609963 1103 3 0.1697165253107542 1103 4 0.023770969254769625 1103 5 -0.049454535256479915 1103 6 0.07735140065528867 1103 7 0.15839747015030525 1103 8 0.04774110440074253 1103 9 0.07987010168549347 1103 10-11 0.0692506909832602 1103 12-13 0.006051817549689531 1103 14-15 0.07790927190659147 1103 16-17 0.06255038915002586 1103 18-19 0.07245200962286447 1103 20-21 0.006396790635164962 1103 22-23 0.05506893847442029 1103 24-25 0.024928129421844858 1103 26-27 0.00539799338094582 1103 28-29 0.08327457319269627 1103 30-31 -0.03297119836570772 1103 32-33 0.05797524475885041 1103 34-35 0.0404036086403039 1103 36-37 0.052762502706457326 1103 38-39 0.051513103984717645 1103 40-41 0.021674581134142556 1103 42-43 0.13408885186604635 1103 44-45 0.05679782570148717 1103 46-47 0.039333009695106114 1103 48-49 0.06787705165582025 1103 50-51 -0.0024469574456702503 1103 52-53 0.05384292005953739 1103 54-55 0.05825348710447287 1103 56-57 0.006107864692253884 1103 58-59 0.017069369890563735 1103 60-61 0.03069516089936286 1103 62-63 -0.0965450977708997 1103 64-65 -0.13542293040848108 1103 66-67 -0.10812883032118492 1103 68-69 -0.04699608050901816 1103 70-71 0.01818471745642114 1103 72-73 -0.0840283428684856 1103 74-75 -0.21660218135487241 1103 76-77 -0.12957733887137124 1103 78-79 -0.2675837208345655 1103 80-81 -0.07315355813918245 1103 82-83 -0.15779601973725477 1103 84-85 0.18746294532236618 1103 86-87 0.20321468433981948 1103 88-89 0.055055214790293405 1103 90-91 0.2456651316465468 1104 1 0.3104449038062356 1104 2 0.10452944140210008 1104 3 0.12135271721138707 1104 4 0.0589724499469213 1104 5 0.009511926970816376 1104 6 0.17413476580534137 1104 7 0.07605547263352008 1104 8 0.056281843743335 1104 9 -0.05170299833051217 1104 10-11 -0.03433086057376755 1104 12-13 -0.06882300614424963 1104 14-15 -0.03884535864112593 1104 16-17 0.08550661268420612 1104 18-19 0.07206574903155882 1104 20-21 -0.009234817108833226 1104 22-23 -0.05309079660656124 1104 24-25 -0.06308712030558894 1104 26-27 0.013002946754909317 1104 28-29 0.022565159822981684 1104 30-31 -0.044148066692251575 1104 32-33 -0.007092611980105801 1104 34-35 -0.037633602904229235 1104 36-37 -0.12600331665264264 1104 38-39 -0.21115203992721376 1104 40-41 0.041169135977312976 1104 42-43 -0.04790545061277385 1104 44-45 -0.0687159193050988 1104 46-47 -0.16046067591253177 1104 48-49 -0.0500140415996313 1104 50-51 -0.13785943585564553 1104 52-53 -0.04090462313610743 1104 54-55 -0.04246164747154779 1104 56-57 -0.08067172017238278 1104 58-59 -0.09551833183758873 1104 60-61 -0.13974087280011105 1104 62-63 -0.06839136423436543 1104 64-65 -0.24844578564332664 1104 66-67 -0.044441160379953715 1104 68-69 -0.1317951010271372 1104 70-71 -0.09500084700659528 1104 72-73 0.01291110894090508 1104 74-75 -0.16407057190902208 1104 76-77 -0.03191446981245605 1104 78-79 -0.22639793192414004 1104 80-81 -0.18945960163861741 1104 82-83 -0.022921306003802044 1104 84-85 -0.27139060464124043 1104 86-87 -0.007896001948608955 1104 88-89 0.20493114531138445 1104 90-91 0.6012206872021011 1201 1 0.3450905179615589 1201 2 0.2064557372511615 1201 3 0.13485593267061802 1201 4 0.19891960512401852 1201 5 0.17068342235117484 1201 6 0.1785953634866928 1201 7 -0.009313424307158868 1201 8 0.09166269669482574 1201 9 0.11052554249236834 1201 10-11 0.07670584102729094 1201 12-13 0.03985899450978536 1201 14-15 0.07356833274182861 1201 16-17 0.05927601885411349 1201 18-19 0.05371606588447264 1201 20-21 0.05114974183355514 1201 22-23 0.06003504096600665 1201 24-25 0.09862854117468345 1201 26-27 0.09027900247811971 1201 28-29 0.0870058233704114 1201 30-31 0.06770060075260886 1201 32-33 0.048181686373908406 1201 34-35 0.13724710761803038 1201 36-37 0.12077202409591337 1201 38-39 0.00976424915768348 1201 40-41 -0.002874628434682336 1201 42-43 0.11886999685683008 1201 44-45 0.037406111352083826 1201 46-47 0.07460391652188747 1201 48-49 0.14618731590785927 1201 50-51 0.06336655822288861 1201 52-53 0.12672379359614183 1201 54-55 0.11464468457850074 1201 56-57 0.13094153944713582 1201 58-59 0.08076407746791858 1201 60-61 0.17124900109862295 1201 62-63 8.659855179686815E-4 1201 64-65 -0.02924857407936088 1201 66-67 0.083735130868682 1201 68-69 -0.06157947654125451 1201 70-71 -0.2808049799219674 1201 72-73 -0.009696541796586189 1201 74-75 -0.11873748539063911 1201 76-77 -0.044215413516781155 1201 78-79 0.07684209637790218 1201 80-81 0.08771321930841225 1201 82-83 0.05678277644384622 1201 84-85 0.05503477120019795 1201 86-87 0.27619791819950734 1201 88-89 0.03241300006563108 1201 90-91 0.2746900749572063 1202 1 0.16038992416878983 1202 2 0.14103770620307898 1202 3 -0.14285159575145912 1202 4 0.16761637979320199 1202 5 0.09165849866679565 1202 6 0.0688714512846147 1202 7 -0.020275696729058268 1202 8 0.09413818842325128 1202 9 0.06367984296358031 1202 10-11 0.024820979942127508 1202 12-13 0.023707946870850094 1202 14-15 -0.10832429452103298 1202 16-17 -0.018278426605029097 1202 18-19 -0.07481543989752026 1202 20-21 -0.007716367311417116 1202 22-23 -0.047288547723638885 1202 24-25 -0.01724868455438866 1202 26-27 -0.0663581883700104 1202 28-29 -0.02713747399180022 1202 30-31 0.016472377400368998 1202 32-33 -0.009352695547619305 1202 34-35 -0.0026895396783714887 1202 36-37 0.03596404894524596 1202 38-39 -0.05298326022878541 1202 40-41 0.05304320411359953 1202 42-43 0.018588759225863782 1202 44-45 0.012002037073678196 1202 46-47 0.0666966152441546 1202 48-49 0.07228239168842521 1202 50-51 -0.03087927143673852 1202 52-53 -0.06045833742907547 1202 54-55 0.01630033146107479 1202 56-57 -0.012552907555097192 1202 58-59 -0.06631520750013209 1202 60-61 0.043270323029773294 1202 62-63 -0.07773094890607268 1202 64-65 -0.05797098418938873 1202 66-67 -0.010051281998251227 1202 68-69 -0.182097649528572 1202 70-71 -0.12512482959004956 1202 72-73 -0.19799065878320476 1202 74-75 -0.171617125332034 1202 76-77 -0.0905380493218928 1202 78-79 -0.10708454764477437 1202 80-81 -0.0414200485433156 1202 82-83 0.10367216910662336 1202 84-85 0.08974406629379672 1202 86-87 -0.10315199208780967 1202 88-89 -0.6568037595686818 1202 90-91 0.1594698454512553 1203 1 0.13321005540546338 1203 2 0.2304094449831524 1203 3 0.1366054960435008 1203 4 0.11794443559014667 1203 5 -0.07666127919067378 1203 6 0.13006115292775178 1203 7 0.01929057111622967 1203 8 0.006215142893864822 1203 9 -0.034298626927757425 1203 10-11 -0.0044364499051852135 1203 12-13 -0.04355363163767123 1203 14-15 -0.02286933987166151 1203 16-17 0.014351271104096952 1203 18-19 0.024845395025032246 1203 20-21 0.07802861314235088 1203 22-23 -0.009565810238989059 1203 24-25 0.016048646813374035 1203 26-27 -0.03950316286532285 1203 28-29 -0.07397053937431508 1203 30-31 -0.022069826558841044 1203 32-33 -0.05561830613822849 1203 34-35 0.03346369549500139 1203 36-37 -0.046265324073225145 1203 38-39 0.054190941620696265 1203 40-41 -0.01371509816944183 1203 42-43 -0.09242559099033087 1203 44-45 -1.9888726539107893E-4 1203 46-47 -0.03329594883204834 1203 48-49 -0.1180899878217403 1203 50-51 -0.08203277816592447 1203 52-53 -0.0025489318676577 1203 54-55 -0.24870146030901452 1203 56-57 -0.07780548799026832 1203 58-59 -0.09005501221275836 1203 60-61 -0.09795660658696903 1203 62-63 -0.21077453436898708 1203 64-65 -0.34078928113260076 1203 66-67 -0.24946380547206104 1203 68-69 -0.045561471881065074 1203 70-71 -0.08266858468135752 1203 72-73 -0.06922756801647978 1203 74-75 -0.16306713948091556 1203 76-77 -0.18570268354408626 1203 78-79 -0.2504277556782455 1203 80-81 -0.1185219308103811 1203 82-83 -0.08955009368116862 1203 84-85 -0.3468478493922049 1203 86-87 -0.163401337860563 1203 88-89 -0.09536879533083464 1203 90-91 0.07212015810156913 1204 1 0.19864614245452117 1204 2 0.04971054945536224 1204 3 -0.15059539592024862 1204 4 -0.20327686862095362 1204 5 -0.06978731354143974 1204 6 -0.12695654975735238 1204 7 -0.07136064501026596 1204 8 -0.069493774520069 1204 9 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0.09812831704235236 2401 42-43 0.11365548593332164 2401 44-45 0.11629968675918434 2401 46-47 0.10749351966670417 2401 48-49 0.08592505596028133 2401 50-51 0.024381688545474844 2401 52-53 0.10322692721169346 2401 54-55 0.073263467689749 2401 56-57 0.10016750619059422 2401 58-59 0.18699432883116884 2401 60-61 0.2073197578728454 2401 62-63 0.0923631922611392 2401 64-65 0.14795032521487883 2401 66-67 0.07391412704050282 2401 68-69 0.09774588822637753 2401 70-71 0.17652982802273698 2401 72-73 0.03049271014443633 2401 74-75 0.14320269853249812 2401 76-77 0.30487192853460243 2401 78-79 0.36907634752625285 2401 80-81 0.333439511251612 2401 82-83 0.1580726616297241 2401 84-85 0.03757616460971036 2401 86-87 0.10699024861980178 2401 88-89 0.5058659577827171 2401 90-91 0.6012206872021011 2402 1 0.2918235303687524 2402 2 0.28357298331779646 2402 3 0.21008408054870387 2402 4 0.23738032993097846 2402 5 0.25240706128451507 2402 6 0.19618463654173013 2402 7 0.15932718567756154 2402 8 0.1727228782228991 2402 9 0.07607616937860229 2402 10-11 0.16015203863767624 2402 12-13 0.1687866826037876 2402 14-15 0.10615876482184206 2402 16-17 0.10859151895650854 2402 18-19 0.046417809373110686 2402 20-21 0.009553348089134772 2402 22-23 0.02139562376427051 2402 24-25 0.08580139793495078 2402 26-27 0.06695266830534052 2402 28-29 0.10193023918805721 2402 30-31 0.1727377458152688 2402 32-33 0.10449499287625486 2402 34-35 0.11113493604098323 2402 36-37 0.1362747827969315 2402 38-39 0.11853834472564984 2402 40-41 0.09628194707849502 2402 42-43 0.09724402694011758 2402 44-45 0.12059352697466608 2402 46-47 0.13013814898282305 2402 48-49 0.1626392421629319 2402 50-51 0.1473629674913397 2402 52-53 0.04427833090375799 2402 54-55 0.1498577027336978 2402 56-57 0.20479634283747572 2402 58-59 0.12015063575817919 2402 60-61 0.13765009178275278 2402 62-63 0.14600793134760437 2402 64-65 0.2212088284383853 2402 66-67 0.10382688238000526 2402 68-69 0.24513994801192496 2402 70-71 0.19584884793805202 2402 72-73 0.1663767963409981 2402 74-75 0.18491235797703354 2402 76-77 0.17628783799636238 2402 78-79 0.34484632179675145 2402 80-81 0.1898018622635007 2402 82-83 0.24336340670183176 2402 84-85 0.23830619191129898 2402 86-87 0.08033374013022154 2402 88-89 0.40361537258122127 2402 90-91 0.33092210155516 2403 1 -0.2518386001445947 2403 2 -0.10812392671821414 2403 3 4.1606050005782436E-4 2403 4 0.10620057798998062 2403 5 0.13282639845125743 2403 6 0.17150942709142925 2403 7 0.10903489178664927 2403 8 0.12903244076083098 2403 9 0.05850389159871128 2403 10-11 0.08180122118651667 2403 12-13 0.1990720194635216 2403 14-15 0.11647545057105191 2403 16-17 0.05592144137293786 2403 18-19 0.055152545097470806 2403 20-21 0.019690756496444806 2403 22-23 0.049258956125875386 2403 24-25 0.07044692029673172 2403 26-27 0.005648548869721992 2403 28-29 0.014713150240872608 2403 30-31 0.052858944621590354 2403 32-33 0.10545597552935249 2403 34-35 0.03441508074421051 2403 36-37 0.0383075861241835 2403 38-39 0.044404660985840394 2403 40-41 0.058581492148789494 2403 42-43 -0.002869981178669434 2403 44-45 0.04418973832061113 2403 46-47 0.08520684017237556 2403 48-49 0.0396069059844919 2403 50-51 0.06325065937657115 2403 52-53 0.09401474043494318 2403 54-55 0.06744029760007919 2403 56-57 0.02659890319897329 2403 58-59 0.12121101295063141 2403 60-61 0.08289594272815748 2403 62-63 0.17528046710328482 2403 64-65 0.17972817837481614 2403 66-67 0.20402925335273636 2403 68-69 0.18131684798922265 2403 70-71 0.2020655455907132 2403 72-73 0.38336563963358117 2403 74-75 0.355871915816536 2403 76-77 0.27667066296181275 2403 78-79 0.2348510050760808 2403 80-81 0.17038213455363405 2403 82-83 0.33362810592876 2403 84-85 0.2972012730629672 2403 86-87 0.23726219263589599 2403 88-89 -0.027580644648075747 2403 90-91 0.6012206872021011 2404 1 -0.4806626783454817 2404 2 -0.2767306645724261 2404 3 -0.3284810619588967 2404 4 -0.3089806592643214 2404 5 -0.20443756018450898 2404 6 -0.14541416600539492 2404 7 -0.10349567980912866 2404 8 0.002842989962616116 2404 9 -6.014062867194525E-4 2404 10-11 0.012582321115907291 2404 12-13 0.03138347530180141 2404 14-15 0.0648782118737401 2404 16-17 -0.06753271396048177 2404 18-19 -0.04698717945009889 2404 20-21 -0.010017169989637864 2404 22-23 -0.025699115392264105 2404 24-25 -0.030061751918616153 2404 26-27 0.008437588799310447 2404 28-29 0.03561790356298644 2404 30-31 -0.04129483459983163 2404 32-33 0.09604185168328172 2404 34-35 0.03394177483870919 2404 36-37 -0.007229147959350257 2404 38-39 0.06159637568988785 2404 40-41 0.057255731771817864 2404 42-43 0.149645732603652 2404 44-45 0.07270419568784092 2404 46-47 0.12228804443623886 2404 48-49 0.01765602506831243 2404 50-51 0.0941004186721699 2404 52-53 0.05892684157174699 2404 54-55 0.16373634683835547 2404 56-57 0.1951400944768693 2404 58-59 0.12580183522657507 2404 60-61 0.04830910370517216 2404 62-63 0.15608076807105675 2404 64-65 0.1088792359651265 2404 66-67 0.10099952200285855 2404 68-69 0.05287841328766518 2404 70-71 -0.031846160014822544 2404 72-73 -0.04455690809876245 2404 74-75 0.0960409059702485 2404 76-77 0.024771900251153056 2404 78-79 -0.1771995241325044 2404 80-81 0.23810031423145972 2404 82-83 0.010506784144105552 2404 84-85 -0.18282540045818507 2404 86-87 -0.020997994563785483 2404 88-89 -0.00450237409345533 2404 90-91 -0.599483538150011 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 17.0 31 510.0 32 3951.0 33 6787.0 34 8934.0 35 11154.0 36 15912.0 37 24659.0 38 65213.0 39 416308.0 40 365927.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.813013604014092 14.247286974322382 25.420047184946938 32.51965223671659 2 25.21328705179261 19.25658734190434 27.00867289386175 28.521452712441302 3 25.33259513945012 19.760246519049645 21.3264430403291 33.58071530117114 4 26.646014888423835 21.632266373132964 19.505053449528592 32.21666528891461 5 26.776429997868114 21.753762350822083 19.860839790639698 31.608967860670113 6 26.814499462676693 24.747218753670985 22.98666916112303 25.45161262252929 7 24.673799071540138 24.43700700043073 23.443829048524428 27.44536487950471 8 23.377914489455843 26.032878965206685 24.48149388930705 26.10771265603042 9 23.404019265324592 25.96500654794795 24.93060480414892 25.700369382578543 10-11 23.2922038086868 25.038939624004215 24.74542405033001 26.92343251697898 12-13 23.832627054119552 24.62893148801573 24.437170155279908 27.101271302584806 14-15 24.26602071849045 24.08002419042564 24.343845581549147 27.31010950953477 16-17 24.31192161605966 23.775142162258586 24.192981731007688 27.71995449067407 18-19 24.30653750603673 24.068712120882516 24.092750268661653 27.5320001044191 20-21 24.40127608846038 24.248291224879594 23.926984941895117 27.423447744764907 22-23 24.433036899100692 24.06925597037978 23.95004416057918 27.54766296994035 24-25 24.49144633510701 24.296041210739507 23.82365353741467 27.38885891673882 26-27 24.65150124215225 24.18161527651484 23.838391858790565 27.328491622542344 28-29 24.563288853695784 23.935632148901643 23.982294435767024 27.518784561635552 30-31 24.82285433348144 24.14820793140524 23.819722299116624 27.209215435996697 32-33 24.875429761804934 24.16259891693405 23.736312720949428 27.225658600311593 34-35 24.798768051668752 23.95200092926165 23.88343405033463 27.36579696873497 36-37 24.914550710699974 24.138658449047718 23.798248361812576 27.14854247843973 38-39 25.032881305766725 24.05954710867383 23.833047955891207 27.07452362966824 40-41 25.024177652975816 23.867945832239 23.89832130996242 27.209555204822767 42-43 25.16068836823554 24.05275590250703 23.821502030785513 26.96505369847192 44-45 25.267548636484545 24.088839248495017 23.691370555688625 26.952241559331814 46-47 25.197342279931313 23.803870486246677 23.88375411597638 27.115033117845627 48-49 25.373762394165233 24.04522376492913 23.74131774692364 26.839696093981996 50-51 25.457795462254694 24.033127903741562 23.624860410782958 26.884216223220786 52-53 25.43117204651178 23.843250021760085 23.664091991966497 27.06148593976164 54-55 25.598124431117114 23.89158280237597 23.6055893733747 26.904703393132216 56-57 25.74849751978776 24.017285607455445 23.405780274953422 26.828436597803375 58-59 25.657418718516357 23.859627794433976 23.552168976856272 26.930784510193394 60-61 25.836382120264865 23.9408503632125 23.421235619976372 26.80153189654626 62-63 26.000789939151787 23.88283714087821 23.267257022158365 26.849115897811636 64-65 25.99776249481828 23.701073303266856 23.292154248013468 27.009009953901398 66-67 26.245357138446806 23.84746578998281 23.108998678662527 26.79817839290786 68-69 26.408549633159744 23.76040494425638 23.039177718953745 26.79186770363013 70-71 26.267362377036807 23.730857083748084 23.03118995979373 26.970590579421376 72-73 26.587382849356285 23.753375527022396 22.77731952054598 26.88192210307534 74-75 26.664291241676903 23.760880685171664 22.51050360467099 27.064324468480443 76-77 26.80825095211085 23.6105765335062 22.32396078113605 27.2572117332469 78-79 27.325581395348834 23.691113554339253 21.98696192547623 26.996343124835683 80-81 27.637846914434377 23.46226190733574 21.671026744211186 27.228864434018696 82-83 28.108686561661145 23.10376302264828 21.11366405726593 27.67388635842465 84-85 29.10087410122656 22.660839325156257 20.342238827012547 27.896047746604637 86-87 29.99219968798752 21.377004016330865 19.40203140040495 29.22876489527666 88-89 29.605933412119484 20.87832973362131 19.369167447736256 30.14656940652295 90-91 26.99416661204693 22.855082912761354 20.701972864914467 29.44877761027725 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 3.0 12 6.0 13 9.0 14 11.0 15 10.0 16 11.5 17 21.0 18 39.5 19 61.5 20 88.5 21 132.5 22 191.0 23 288.5 24 432.0 25 625.5 26 886.5 27 1228.0 28 1715.0 29 2332.0 30 3131.5 31 4080.0 32 5203.5 33 6571.0 34 8507.0 35 10804.5 36 13565.0 37 16727.5 38 20113.5 39 24234.0 40 29020.0 41 34202.5 42 39160.5 43 43800.0 44 48679.5 45 53634.0 46 58157.5 47 61977.5 48 64708.0 49 66806.5 50 68030.0 51 68011.0 52 67362.5 53 66020.0 54 64062.5 55 61384.0 56 58080.0 57 55050.0 58 52221.0 59 49000.5 60 45746.5 61 42728.0 62 40054.5 63 37251.5 64 34157.5 65 30973.5 66 27524.5 67 24488.5 68 21629.5 69 18598.0 70 15710.5 71 13023.5 72 10562.0 73 8352.5 74 6511.5 75 5111.5 76 3925.0 77 2868.0 78 2100.0 79 1512.0 80 1051.5 81 726.5 82 490.5 83 328.0 84 207.5 85 130.5 86 85.5 87 55.0 88 33.0 89 20.0 90 14.0 91 10.5 92 6.0 93 3.0 94 3.0 95 3.0 96 2.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0011964688939841543 2 0.007722662861170452 3 0.0011964688939841543 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.987634182120205E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 8456.0 32-33 19129.0 34-35 20890.0 36-37 23303.0 38-39 25907.0 40-41 28115.0 42-43 29944.0 44-45 31964.0 46-47 32885.0 48-49 34178.0 50-51 35184.0 52-53 36199.0 54-55 37031.0 56-57 37031.0 58-59 36902.0 60-61 36384.0 62-63 36383.0 64-65 36144.0 66-67 35419.0 68-69 34510.0 70-71 33907.0 72-73 32770.0 74-75 31596.0 76-77 30394.0 78-79 29017.0 80-81 27839.0 82-83 26369.0 84-85 25054.0 86-87 24071.0 88-89 21945.0 90-91 20452.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.67244213111248 #Duplication Level Percentage of deduplicated Percentage of total 1 99.68546674209787 99.35893915164687 2 0.3028145117571285 0.6036452379914693 3 0.01008419559109618 0.030153492044770608 4 8.867686383261023E-4 0.0035354558314897528 5 7.477819155878367E-4 0.0037266624854060534 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0013052387934372593 0.0 1.0876989945310495E-4 0.0 1.0876989945310495E-4 2 0.0013052387934372593 0.0 1.0876989945310495E-4 0.0 2.175397989062099E-4 3 0.0014140086928903642 0.0 1.0876989945310495E-4 0.0 2.175397989062099E-4 4 0.0014140086928903642 0.0 2.175397989062099E-4 0.0 2.175397989062099E-4 5 0.0014140086928903642 0.0 2.175397989062099E-4 0.0 2.175397989062099E-4 6 0.0014140086928903642 0.0 2.175397989062099E-4 0.0 2.175397989062099E-4 7 0.0017403183912496792 0.0 2.175397989062099E-4 0.0 2.175397989062099E-4 8 0.007287583263358031 0.0 2.175397989062099E-4 0.0 2.175397989062099E-4 9 0.007505123062264241 0.0 2.175397989062099E-4 0.0 2.175397989062099E-4 10-11 0.007505123062264241 0.0 2.175397989062099E-4 0.0 2.175397989062099E-4 12-13 0.007505123062264241 0.0 2.175397989062099E-4 0.0 2.175397989062099E-4 14-15 0.007505123062264241 0.0 2.175397989062099E-4 0.0 2.175397989062099E-4 16-17 0.007505123062264241 0.0 2.175397989062099E-4 0.0 2.175397989062099E-4 18-19 0.007505123062264241 0.0 2.175397989062099E-4 0.0 3.263096983593148E-4 20-21 0.007505123062264241 0.0 2.175397989062099E-4 5.4384949726552474E-5 4.350795978124198E-4 22-23 0.007559508011990793 0.0 2.175397989062099E-4 1.0876989945310495E-4 4.350795978124198E-4 24-25 0.007613892961717346 0.0 2.175397989062099E-4 1.0876989945310495E-4 4.350795978124198E-4 26-27 0.007722662861170451 0.0 2.175397989062099E-4 1.0876989945310495E-4 4.350795978124198E-4 28-29 0.007777047810897004 1.0876989945310495E-4 2.175397989062099E-4 1.0876989945310495E-4 4.350795978124198E-4 30-31 0.007994587609803212 1.0876989945310495E-4 2.175397989062099E-4 1.0876989945310495E-4 5.438494972655248E-4 32-33 0.008157742458982872 1.0876989945310495E-4 2.175397989062099E-4 1.0876989945310495E-4 5.982344469920773E-4 34-35 0.008157742458982872 1.0876989945310495E-4 2.175397989062099E-4 1.0876989945310495E-4 6.526193967186296E-4 36-37 0.008157742458982872 1.0876989945310495E-4 3.263096983593148E-4 1.0876989945310495E-4 7.070043464451821E-4 38-39 0.008375282257889081 1.0876989945310495E-4 3.263096983593148E-4 1.0876989945310495E-4 7.613892961717346E-4 40-41 0.008375282257889081 1.0876989945310495E-4 3.263096983593148E-4 1.0876989945310495E-4 8.157742458982871E-4 42-43 0.008375282257889081 1.0876989945310495E-4 3.263096983593148E-4 1.0876989945310495E-4 8.701591956248396E-4 44-45 0.008538437107068739 1.0876989945310495E-4 3.263096983593148E-4 1.0876989945310495E-4 9.245441453513921E-4 46-47 0.00859282205679529 1.0876989945310495E-4 3.263096983593148E-4 1.0876989945310495E-4 9.789290950779446E-4 48-49 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