##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-3A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 648321 Sequences flagged as poor quality 0 Sequence length 31-91 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.28724196809914 40.0 40.0 40.0 40.0 40.0 2 39.14756424672346 40.0 40.0 40.0 40.0 40.0 3 39.12638029618044 40.0 40.0 40.0 40.0 40.0 4 39.05388842872589 40.0 40.0 40.0 40.0 40.0 5 39.192511117178064 40.0 40.0 40.0 40.0 40.0 6 39.26888069336023 40.0 40.0 40.0 40.0 40.0 7 39.311205405964024 40.0 40.0 40.0 40.0 40.0 8 39.30204327794411 40.0 40.0 40.0 40.0 40.0 9 39.34054272497729 40.0 40.0 40.0 40.0 40.0 10-11 39.32840676146539 40.0 40.0 40.0 40.0 40.0 12-13 39.33831851814148 40.0 40.0 40.0 40.0 40.0 14-15 39.347462136811856 40.0 40.0 40.0 40.0 40.0 16-17 39.48287345311967 40.0 40.0 40.0 40.0 40.0 18-19 39.49019081596925 40.0 40.0 40.0 40.0 40.0 20-21 39.49645006100373 40.0 40.0 40.0 40.0 40.0 22-23 39.49934677420599 40.0 40.0 40.0 40.0 40.0 24-25 39.50285738083449 40.0 40.0 40.0 40.0 40.0 26-27 39.49353175356035 40.0 40.0 40.0 40.0 40.0 28-29 39.491289037375005 40.0 40.0 40.0 40.0 40.0 30-31 39.48425239966004 40.0 40.0 40.0 40.0 40.0 32-33 39.48540784509927 40.0 40.0 40.0 40.0 40.0 34-35 39.48456094845616 40.0 40.0 40.0 40.0 40.0 36-37 39.47830443723652 40.0 40.0 40.0 40.0 40.0 38-39 39.46224441543585 40.0 40.0 40.0 40.0 40.0 40-41 39.45076880919071 40.0 40.0 40.0 40.0 40.0 42-43 39.451236588516004 40.0 40.0 40.0 40.0 40.0 44-45 39.42969062130855 40.0 40.0 40.0 40.0 40.0 46-47 39.42269292336654 40.0 40.0 40.0 40.0 40.0 48-49 39.429169785448266 40.0 40.0 40.0 40.0 40.0 50-51 39.42516480931164 40.0 40.0 40.0 40.0 40.0 52-53 39.41409963410909 40.0 40.0 40.0 40.0 40.0 54-55 39.39428328000916 40.0 40.0 40.0 40.0 40.0 56-57 39.38920031004015 40.0 40.0 40.0 40.0 40.0 58-59 39.37518866854471 40.0 40.0 40.0 40.0 40.0 60-61 39.349249700255854 40.0 40.0 40.0 40.0 40.0 62-63 39.361423077440925 40.0 40.0 40.0 40.0 40.0 64-65 39.37145876681275 40.0 40.0 40.0 40.0 40.0 66-67 39.35604581281733 40.0 40.0 40.0 40.0 40.0 68-69 39.357637255416066 40.0 40.0 40.0 40.0 40.0 70-71 39.34647899387515 40.0 40.0 40.0 40.0 40.0 72-73 39.34606644129133 40.0 40.0 40.0 40.0 40.0 74-75 39.33983336303891 40.0 40.0 40.0 40.0 40.0 76-77 39.32401827507442 40.0 40.0 40.0 40.0 40.0 78-79 39.313952707641434 40.0 40.0 40.0 40.0 40.0 80-81 39.323388048304295 40.0 40.0 40.0 40.0 40.0 82-83 39.291824201643166 40.0 40.0 40.0 40.0 40.0 84-85 39.30961745436857 40.0 40.0 40.0 40.0 40.0 86-87 39.32743542241852 40.0 40.0 40.0 40.0 40.0 88-89 39.38107951723918 40.0 40.0 40.0 40.0 40.0 90-91 39.458559099530206 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16919652262781426 1101 2 0.27275344622061226 1101 3 0.24479349718264842 1101 4 0.20652270955737606 1101 5 0.025587147808856514 1101 6 0.16058463548553448 1101 7 0.22950078113053252 1101 8 0.11766449267118162 1101 9 0.06725447177415589 1101 10-11 0.1274810295609896 1101 12-13 0.08255168625301224 1101 14-15 0.20351632997303426 1101 16-17 0.08563437570410315 1101 18-19 0.14174179275610044 1101 20-21 0.0388300532622452 1101 22-23 0.1780575800792903 1101 24-25 0.16348242181368988 1101 26-27 0.018758296809899377 1101 28-29 0.10531285069266261 1101 30-31 0.11096156462381401 1101 32-33 0.19592048457203504 1101 34-35 0.08194652077590803 1101 36-37 0.06980046677699647 1101 38-39 0.1252974186165261 1101 40-41 0.022510087475389184 1101 42-43 0.10161547316268127 1101 44-45 0.17446870064797082 1101 46-47 0.2495070586033279 1101 48-49 0.015679677315326046 1101 50-51 0.01884409139334764 1101 52-53 0.18113305389756817 1101 54-55 0.11776918569448469 1101 56-57 0.03145847227248311 1101 58-59 0.06685601233294847 1101 60-61 0.2004981494658722 1101 62-63 0.08772222528435236 1101 64-65 0.17418540965070406 1101 66-67 0.05862884484499631 1101 68-69 0.15426595817079658 1101 70-71 0.2219417810461053 1101 72-73 -0.0359341442766663 1101 74-75 0.17117364645481814 1101 76-77 -0.02530654909280372 1101 78-79 -0.06844595809543108 1101 80-81 -0.0872418898281282 1101 82-83 -0.13577244613624373 1101 84-85 -0.6277184562923281 1101 86-87 0.17571097877990383 1101 88-89 -0.26856626090094693 1101 90-91 0.729714264899954 1102 1 0.38437450627063896 1102 2 0.03196696135307775 1102 3 0.2209723941703885 1102 4 0.101446528288065 1102 5 0.05208452935573149 1102 6 0.05402723412974808 1102 7 0.008963568255921928 1102 8 0.05741931293795233 1102 9 -0.002797304207248885 1102 10-11 0.0139274950940802 1102 12-13 -0.017921444207047443 1102 14-15 -0.05366514098675168 1102 16-17 0.07665512761234083 1102 18-19 -0.015980716451480248 1102 20-21 -0.004545675486170353 1102 22-23 0.012149810123588622 1102 24-25 0.06715715240658682 1102 26-27 -0.06757429714897967 1102 28-29 -0.1393167998046323 1102 30-31 -0.08730693505888354 1102 32-33 0.021948204740944277 1102 34-35 -0.018908154621762208 1102 36-37 -0.037086367196721426 1102 38-39 0.002120052523970628 1102 40-41 -0.0017054683643564772 1102 42-43 -0.09711907339770676 1102 44-45 0.004495774404745134 1102 46-47 -0.12768766720160585 1102 48-49 -0.01708971298987194 1102 50-51 0.026116576696225025 1102 52-53 -0.14188599485328268 1102 54-55 -0.06802323803007937 1102 56-57 -0.06046982453504057 1102 58-59 -0.021800638709322584 1102 60-61 -0.16271100712609865 1102 62-63 0.012322637116525925 1102 64-65 0.013877179105058701 1102 66-67 0.015964219131809898 1102 68-69 -0.01890484336232845 1102 70-71 0.10069293549995706 1102 72-73 -0.0048663511731135145 1102 74-75 0.06725652132256243 1102 76-77 0.16985462716169764 1102 78-79 0.3420759214918192 1102 80-81 0.34400392867619445 1102 82-83 0.10456605296526789 1102 84-85 -0.2153461728530175 1102 86-87 0.04975634063173828 1102 88-89 -0.009888798012958944 1102 90-91 0.729714264899954 1103 1 0.4312791652989034 1103 2 0.11514037494426788 1103 3 -0.014406123939778581 1103 4 -0.023858536002656194 1103 5 -0.019700562349797224 1103 6 0.013828774723037895 1103 7 -0.06053000267245778 1103 8 0.012289167073262774 1103 9 0.003318880961934667 1103 10-11 -0.10195894202319522 1103 12-13 0.015046358287108319 1103 14-15 0.02690610027850937 1103 16-17 0.06281884172351226 1103 18-19 0.0679317051406656 1103 20-21 0.003683238201872996 1103 22-23 -0.08573001168012695 1103 24-25 -0.005224658460996068 1103 26-27 0.027371841683212494 1103 28-29 -0.024713197140627585 1103 30-31 0.07235157172777207 1103 32-33 0.080902287968037 1103 34-35 0.03887043096742815 1103 36-37 -0.03141997778292449 1103 38-39 0.06242955028347552 1103 40-41 0.06547342600902084 1103 42-43 0.057121526969233116 1103 44-45 -0.017972503318610222 1103 46-47 0.023134393526518693 1103 48-49 0.1127314877832788 1103 50-51 0.018752223717953598 1103 52-53 0.04822615406946085 1103 54-55 0.006538710985815044 1103 56-57 0.12230605914263748 1103 58-59 -0.051965282906664356 1103 60-61 -0.01941280258344591 1103 62-63 -0.2093666802626899 1103 64-65 -0.028339387988694398 1103 66-67 -0.05580741929227173 1103 68-69 0.1151653520213145 1103 70-71 0.1048576421718721 1103 72-73 0.06454879715012396 1103 74-75 0.11606184512882578 1103 76-77 -0.09719120057343389 1103 78-79 0.12973101793961916 1103 80-81 0.0703718234651376 1103 82-83 0.34478557521550357 1103 84-85 0.10416346373044405 1103 86-87 -0.6662868395152515 1103 88-89 0.26673002176375604 1103 90-91 -0.17138463619895106 1104 1 0.2578872332888906 1104 2 0.2534807905679983 1104 3 0.2315511494754432 1104 4 0.20914105652310155 1104 5 0.08641737233790536 1104 6 0.21331652139048174 1104 7 0.09153938436986664 1104 8 0.037028674611541135 1104 9 0.17524390570144277 1104 10-11 -0.041382074918011824 1104 12-13 0.0876116996923102 1104 14-15 0.032121770573631636 1104 16-17 -0.030140585484119242 1104 18-19 0.009683262274371884 1104 20-21 0.07865267763372685 1104 22-23 -0.013385870577614867 1104 24-25 0.07003292347042844 1104 26-27 0.06742073859223296 1104 28-29 0.04287222692363457 1104 30-31 0.060616313563734536 1104 32-33 0.060577876299973354 1104 34-35 -0.030667724406342245 1104 36-37 0.0017717531303276246 1104 38-39 0.009380554866154966 1104 40-41 0.08559071300819454 1104 42-43 0.03337571090887792 1104 44-45 0.07800866570413234 1104 46-47 -0.03775580850099658 1104 48-49 0.13083640425357146 1104 50-51 -0.08925297368111984 1104 52-53 -0.05344855360471712 1104 54-55 0.058604928593794625 1104 56-57 0.03911476849737028 1104 58-59 0.08323636949586444 1104 60-61 0.08047552780916334 1104 62-63 0.03391534406567587 1104 64-65 0.13541973802004748 1104 66-67 0.08539471008330679 1104 68-69 -0.30140872231659444 1104 70-71 -0.12567081268496594 1104 72-73 0.05161683213484736 1104 74-75 -0.01743895125728301 1104 76-77 0.0660799937375316 1104 78-79 -0.019302557262030007 1104 80-81 0.1278493180115916 1104 82-83 0.17575037549994477 1104 84-85 0.10691376499272565 1104 86-87 -0.2578863925749033 1104 88-89 -0.1923918087866454 1104 90-91 -0.8911190684333832 1201 1 0.17589963486808102 1201 2 0.1745545531981989 1201 3 0.2568840515788011 1201 4 0.26178085310152 1201 5 0.13199763687518384 1201 6 0.22918095160929397 1201 7 0.2278675355085369 1201 8 0.1308211935150254 1201 9 0.07825619575564247 1201 10-11 0.10426398936500192 1201 12-13 0.1033869099169209 1201 14-15 0.04062276314606805 1201 16-17 0.098099215555294 1201 18-19 0.04851068850058482 1201 20-21 0.06402059858481834 1201 22-23 0.07110267580563345 1201 24-25 0.08605070055427433 1201 26-27 0.07891617722002309 1201 28-29 0.1426620023122993 1201 30-31 0.07858020177108216 1201 32-33 0.07777599954807357 1201 34-35 0.010974277633692964 1201 36-37 0.012825209587902009 1201 38-39 -0.02412692251597548 1201 40-41 -0.006452923106387232 1201 42-43 0.03950542818160585 1201 44-45 -0.05781408637406571 1201 46-47 -0.05910222700656931 1201 48-49 -0.05399282457783272 1201 50-51 -0.07144433191632515 1201 52-53 -0.09568618743821844 1201 54-55 0.11538522653830086 1201 56-57 -0.009219742713106882 1201 58-59 -0.08612278614138802 1201 60-61 0.08400736535430298 1201 62-63 0.08318351517245759 1201 64-65 -0.049712095630432884 1201 66-67 0.05344179739844179 1201 68-69 -0.030385675829990078 1201 70-71 0.0644236928694184 1201 72-73 0.009004275405203543 1201 74-75 0.11053209833810484 1201 76-77 0.003065309656712145 1201 78-79 0.2738522104462717 1201 80-81 -0.16008100980459972 1201 82-83 0.20559075428746354 1201 84-85 -0.29463864550389474 1201 86-87 -0.09253493511818078 1201 88-89 -0.1217125434229942 1201 90-91 -0.30254379961617417 1202 1 0.21010146902808202 1202 2 0.22489005026430675 1202 3 0.1270587178590219 1202 4 0.003139137718960683 1202 5 0.22677077304816606 1202 6 -0.0010993675367316769 1202 7 0.09599285336944519 1202 8 0.022122331525451955 1202 9 0.11465913128661498 1202 10-11 0.04476999659445369 1202 12-13 0.021641286143932348 1202 14-15 0.0734759100374589 1202 16-17 -0.086361965766244 1202 18-19 -0.02136812788107534 1202 20-21 0.06794703531613777 1202 22-23 0.01884975968809499 1202 24-25 0.03266442230225408 1202 26-27 0.03604186694403211 1202 28-29 0.08233200116389838 1202 30-31 0.16907622885145912 1202 32-33 0.04206921594050783 1202 34-35 0.09029794705170957 1202 36-37 0.14541172586365292 1202 38-39 0.022213618506071953 1202 40-41 0.08967273371428774 1202 42-43 0.031103601571984996 1202 44-45 0.17528496960291307 1202 46-47 0.14741011077406796 1202 48-49 0.022976852301084705 1202 50-51 0.11797347912614953 1202 52-53 0.06925879427124926 1202 54-55 0.12293896914366087 1202 56-57 0.20783378843109546 1202 58-59 0.22717614034463196 1202 60-61 0.07633618005991138 1202 62-63 0.04344665395905167 1202 64-65 0.033514237547130676 1202 66-67 -0.3105377492892103 1202 68-69 0.004222280688324531 1202 70-71 -0.013433355930224877 1202 72-73 0.21929797025805442 1202 74-75 0.030459338480753217 1202 76-77 0.12034843390856054 1202 78-79 0.14047250997796112 1202 80-81 -0.12213163355445289 1202 82-83 -0.0663195847819722 1202 84-85 -0.1354642601841789 1202 86-87 -0.17061226319550826 1202 88-89 0.16833429983862658 1202 90-91 0.729714264899954 1203 1 0.3429198994043361 1203 2 0.1555845905489477 1203 3 0.036297088124157995 1203 4 0.04861674224925849 1203 5 -0.016343200840957195 1203 6 0.1093301924324166 1203 7 -0.04062000253139075 1203 8 0.048698200914905954 1203 9 0.07948725687356983 1203 10-11 0.06695513491398941 1203 12-13 -0.04140668802473613 1203 14-15 0.011996875998292467 1203 16-17 0.024675406211656536 1203 18-19 -0.024327319531707303 1203 20-21 0.004626967130192838 1203 22-23 -0.03915296869743656 1203 24-25 0.07506777054772584 1203 26-27 -0.01870723969753385 1203 28-29 -0.02491703694652614 1203 30-31 0.007791484831358275 1203 32-33 0.05699564791082423 1203 34-35 0.04269642718351463 1203 36-37 -0.003727831382690283 1203 38-39 0.06394008365717241 1203 40-41 -0.02055871147883437 1203 42-43 -0.006599406111646999 1203 44-45 -0.07058353653084737 1203 46-47 -0.03709082897024274 1203 48-49 0.018354158603344217 1203 50-51 0.07055630819066039 1203 52-53 -0.09827014121060529 1203 54-55 -0.05049059155507507 1203 56-57 -0.08974161142310066 1203 58-59 -0.08370574563170408 1203 60-61 -0.0085645602393285 1203 62-63 -8.862639931734861E-4 1203 64-65 -0.0630941749924645 1203 66-67 -0.020220016483101233 1203 68-69 0.08834333590808541 1203 70-71 -0.04661446063106922 1203 72-73 -0.007311489066424315 1203 74-75 0.03470646603867067 1203 76-77 -0.2575095742150353 1203 78-79 -4.1628872333632216E-4 1203 80-81 0.13123256934252936 1203 82-83 -0.1501970476541814 1203 84-85 0.2885242607979208 1203 86-87 -0.41812081614462926 1203 88-89 -0.41129313432544023 1203 90-91 0.729714264899954 1204 1 0.24266033704804357 1204 2 0.126596441416865 1204 3 -0.009203060321652856 1204 4 0.056346647277450757 1204 5 -0.13365359064022186 1204 6 0.02479449272320977 1204 7 0.02300728924739559 1204 8 -0.13555019081046282 1204 9 -0.09767594706282523 1204 10-11 -0.033732675171883386 1204 12-13 -0.10405886173380452 1204 14-15 -0.05169944475724719 1204 16-17 -0.04106296592422609 1204 18-19 -0.0449770363453581 1204 20-21 -0.09239962645064992 1204 22-23 0.0016170193799922572 1204 24-25 0.019870726328306887 1204 26-27 -0.02309024462942233 1204 28-29 -0.03164166561418824 1204 30-31 -0.1170953378050612 1204 32-33 0.015065767569190314 1204 34-35 -0.08478547635677813 1204 36-37 -0.10062503381119825 1204 38-39 0.008360816023554207 1204 40-41 -0.10587703702142193 1204 42-43 -0.05191006421540578 1204 44-45 -0.182554879969814 1204 46-47 -0.12062126943307305 1204 48-49 -0.09795154167083098 1204 50-51 -0.09645079049073502 1204 52-53 -0.05334962336903715 1204 54-55 -0.07992104292880953 1204 56-57 -0.07121744746648062 1204 58-59 -0.04926389659236463 1204 60-61 -0.1364516576801975 1204 62-63 -0.13014998133982658 1204 64-65 -0.008937570949164808 1204 66-67 0.04328265015534072 1204 68-69 -0.029847439413430266 1204 70-71 -0.1297133559873842 1204 72-73 0.1783649514537089 1204 74-75 -0.26222714899962796 1204 76-77 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-0.011784494772726362 2401 54-55 0.024782618130160472 2401 56-57 0.0014890230967878892 2401 58-59 0.014596898698158611 2401 60-61 0.07977586197792164 2401 62-63 -0.03392642453125916 2401 64-65 0.08838915344022524 2401 66-67 1.9842864498542667E-4 2401 68-69 -0.043904154826932995 2401 70-71 -0.08083078792561338 2401 72-73 -0.06903540128209329 2401 74-75 0.040141002004176585 2401 76-77 -0.0490351561661555 2401 78-79 -0.020390355969645668 2401 80-81 0.27652872962204356 2401 82-83 -0.06670327031098822 2401 84-85 0.13159058016188396 2401 86-87 -0.5772722698555128 2401 88-89 0.30793541830895776 2401 90-91 0.1873413835440232 2402 1 0.2909808897046773 2402 2 0.21719101965453547 2402 3 0.29313620944534335 2402 4 0.21174811305746744 2402 5 0.14714734816752184 2402 6 0.23438959498092515 2402 7 0.1746649639268938 2402 8 0.08425592177265884 2402 9 0.18838179847008973 2402 10-11 0.17780259285330402 2402 12-13 0.10028404665125379 2402 14-15 0.10447328996313843 2402 16-17 0.045462153825425844 2402 18-19 0.07535752462178635 2402 20-21 -0.024384961269930727 2402 22-23 0.005310069102847592 2402 24-25 0.06929375073583088 2402 26-27 0.04040197994638106 2402 28-29 0.05762717881089685 2402 30-31 0.13667889812204947 2402 32-33 -0.01666733924228936 2402 34-35 0.06626789996138172 2402 36-37 -0.0019655433725063176 2402 38-39 -0.06463217030222523 2402 40-41 -0.0022023178918786357 2402 42-43 0.010662352468820302 2402 44-45 -0.014749860354598354 2402 46-47 0.09818771866176945 2402 48-49 0.01424549443132861 2402 50-51 0.053127610275439 2402 52-53 0.09184492706999947 2402 54-55 0.11818356625482096 2402 56-57 0.12379168631211002 2402 58-59 0.14951961865368446 2402 60-61 0.06067894581634903 2402 62-63 0.2286457757932041 2402 64-65 0.17802232388988415 2402 66-67 0.10756887906543255 2402 68-69 0.011087371061961449 2402 70-71 0.274860605339029 2402 72-73 0.17605101416312152 2402 74-75 0.2674950513257528 2402 76-77 0.1613417591894546 2402 78-79 0.03517996882362695 2402 80-81 0.2792921175994394 2402 82-83 0.4474151646948741 2402 84-85 0.5804895523733649 2402 86-87 0.4360117668681269 2402 88-89 0.10731688588003152 2402 90-91 0.729714264899954 2403 1 -0.32692330713678075 2403 2 -0.11999159966315176 2403 3 -0.11811000442281028 2403 4 0.1409402802829618 2403 5 0.06905892407089453 2403 6 0.15218971214846277 2403 7 0.13630069753278917 2403 8 0.06207199366865268 2403 9 0.04313082311266925 2403 10-11 0.06854604580555446 2403 12-13 0.029265249986792696 2403 14-15 0.10362320208748343 2403 16-17 0.13003997675286882 2403 18-19 0.0460974251540307 2403 20-21 0.04930874533756935 2403 22-23 0.005100974907627176 2403 24-25 0.004500414327885949 2403 26-27 0.04505596177294535 2403 28-29 0.09812819467837386 2403 30-31 0.0798261552798678 2403 32-33 -0.012105826410866882 2403 34-35 -0.02052529801679981 2403 36-37 -0.015620912053648794 2403 38-39 0.01705925121150642 2403 40-41 0.022451186487472796 2403 42-43 0.01927707137585344 2403 44-45 -0.013289958078118502 2403 46-47 0.12629636508750508 2403 48-49 0.03403430159200127 2403 50-51 0.13854501646939354 2403 52-53 0.09663317110602776 2403 54-55 0.018053948185148272 2403 56-57 0.022862095981864172 2403 58-59 0.19169825175904975 2403 60-61 0.04999856321550311 2403 62-63 0.1887807257221965 2403 64-65 0.07408679798134443 2403 66-67 0.14998100511044044 2403 68-69 0.2857501513770231 2403 70-71 0.09333674940511827 2403 72-73 -0.07261285285478891 2403 74-75 0.08838546005726755 2403 76-77 0.08209352815009652 2403 78-79 -0.0731845162330984 2403 80-81 0.03625124371468047 2403 82-83 0.19953772461580144 2403 84-85 0.20635768428535783 2403 86-87 -0.2522320846917836 2403 88-89 -0.19892654389280295 2403 90-91 -0.2513155453981426 2404 1 -0.42346749150525653 2404 2 -0.37167468354949307 2404 3 -0.2190447473411723 2404 4 -0.339064967396574 2404 5 -0.12897975570039222 2404 6 -0.1084144269587739 2404 7 -0.1330233357462447 2404 8 -0.09444004866262645 2404 9 -0.04103321060367193 2404 10-11 -0.009312026328181844 2404 12-13 0.047839849031277026 2404 14-15 0.1470411906956457 2404 16-17 -0.0051380617082514846 2404 18-19 0.025603050010538198 2404 20-21 0.02465339762413521 2404 22-23 -0.019827689448604247 2404 24-25 -0.0769851162890447 2404 26-27 -0.02135998242673054 2404 28-29 -0.020871685062303413 2404 30-31 0.05593828069941509 2404 32-33 0.10542940741753881 2404 34-35 0.012486388045452657 2404 36-37 -0.004062761652917857 2404 38-39 0.003545094226254264 2404 40-41 0.0209086490814272 2404 42-43 0.08616174818278921 2404 44-45 0.09206203157776827 2404 46-47 0.07348923842845068 2404 48-49 0.04710707306384876 2404 50-51 0.02451212492632493 2404 52-53 0.0303322980146703 2404 54-55 -0.11087810925592123 2404 56-57 -0.04215443124747509 2404 58-59 0.007224881654600779 2404 60-61 3.811386673930883E-4 2404 62-63 0.039521839604681475 2404 64-65 0.0960929341117307 2404 66-67 0.14954876131715622 2404 68-69 0.09576147737195129 2404 70-71 -0.05714585088283286 2404 72-73 0.04194582913213907 2404 74-75 -0.004389274123369091 2404 76-77 -0.09207773812988762 2404 78-79 -0.28545664217070055 2404 80-81 0.19891772585619094 2404 82-83 0.26166333742796155 2404 84-85 0.31597861615057354 2404 86-87 0.29583980325655546 2404 88-89 0.2900217627025583 2404 90-91 0.32619520331637375 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 7.0 31 375.0 32 2484.0 33 4498.0 34 6149.0 35 7426.0 36 11104.0 37 17151.0 38 45596.0 39 286549.0 40 266982.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.253600531846793 14.441306356402137 26.81954691218821 31.485546199562865 2 25.298985769927885 18.998881647448997 27.633643129844586 28.06848945277853 3 25.313427402686344 19.760425471529317 22.105886635529586 32.82026049025475 4 26.447392572506523 21.5326975371768 20.37524621290996 31.644663677406715 5 26.770226477316022 21.436140430434925 20.511598421152485 31.28203467109657 6 26.812952225826404 24.304626874650058 23.55515246305457 25.32726843646897 7 24.676819044886717 24.271927023804565 23.961124196192934 27.09012973511578 8 23.41355593911041 25.684653127077482 24.80931513864274 26.092475795169367 9 23.314685163676636 25.719820891194335 25.21235622477137 25.75313772035766 10-11 23.200544174876335 24.859444626967196 25.07284200264992 26.867169195506545 12-13 23.691736038166276 24.426480092423354 24.78563859569565 27.09614527371472 14-15 24.106114100885208 23.925185209178785 24.642962359695275 27.32573833024073 16-17 24.107965035838728 23.73322783004098 24.503987993601935 27.654819140518356 18-19 24.127476975140404 24.02667814246338 24.402109448868693 27.44373543352753 20-21 24.28048759796459 24.12793970887878 24.149225460844242 27.442347232312386 22-23 24.15038229519019 23.919401037449042 24.235216813893118 27.69499985346765 24-25 24.363702548583184 24.250487027259645 24.055213389663454 27.330597034493714 26-27 24.546636619822586 24.11907064555984 24.053825188448315 27.280467546169255 28-29 24.37203175587402 23.948476140677226 24.147605892759913 27.53188621068884 30-31 24.688464510635935 24.08428849289164 24.06971238013268 27.157534616339746 32-33 24.800320272545655 24.061024479655234 23.94547516906544 27.19318007873367 34-35 24.70664602383602 23.849484735594366 24.02543261244698 27.418436628122635 36-37 24.942114474083496 23.98959099770952 23.971716113584495 27.096578414622485 38-39 24.986677663154506 23.93612854182611 24.003622642549004 27.07357115247038 40-41 24.90683785937486 23.789608459355165 24.158675584445817 27.14487809682416 42-43 25.06026981794165 24.060684246026216 23.96498223010956 26.91406370592258 44-45 25.35297885467056 23.849699579922742 23.836535325392866 26.960786240013835 46-47 25.15441723422727 23.802631878844892 23.953267677950194 27.089683208977643 48-49 25.34970063298504 23.873613767150207 23.93761485615673 26.83907074370802 50-51 25.514958241259993 23.841453510316693 23.752525565323477 26.891062683099836 52-53 25.515149166698958 23.676174452601277 23.857796955291867 26.950879425407905 54-55 25.63104032185507 23.849476891509543 23.780857089526684 26.738625697108702 56-57 25.846981624633457 23.728531028814697 23.64909819087363 26.775389155678216 58-59 25.721658557437234 23.686179107104692 23.73489734595607 26.857264989502006 60-61 26.021113743102433 23.64444840003834 23.463023564689937 26.871414292169295 62-63 26.121963527626296 23.595235817289204 23.432215074822583 26.85058558026192 64-65 26.289320926614728 23.452711452769375 23.4847763935837 26.773191227032196 66-67 26.45916362100314 23.461633822476077 23.23229076750721 26.84691178901357 68-69 26.510924852821162 23.499083821363133 23.19651390179181 26.793477424023898 70-71 26.573248555047062 23.446711755755224 23.014238355038966 26.965801334158744 72-73 26.934889637309112 23.39497914016622 22.866727741272154 26.803403481252513 74-75 27.00499275613067 23.35149687620018 22.74156688125758 26.901943486411568 76-77 26.967387096343565 23.265117231129327 22.597640692929645 27.169854979597467 78-79 27.401349741961095 23.424639407172158 22.235807860262007 26.938202990604736 80-81 27.70085596506685 23.026810806639357 21.840292738766664 27.432040489527125 82-83 28.599436837901294 22.59022292505426 21.441307697893386 27.36903253915106 84-85 29.14155348047538 22.12966893039049 20.58892190152801 28.139855687606115 86-87 29.835186356925487 21.455904064599718 19.812287203591552 28.896622374883247 88-89 29.787128835265904 20.693747373105545 19.33147081365738 30.187652977971176 90-91 27.537682524729156 22.008949599623175 20.949128591615636 29.50423928403203 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.5 11 2.0 12 2.5 13 5.0 14 7.0 15 9.0 16 11.5 17 15.5 18 28.5 19 49.0 20 74.5 21 110.5 22 172.5 23 264.5 24 374.5 25 504.0 26 698.0 27 1001.5 28 1410.0 29 1861.5 30 2415.5 31 3206.5 32 4150.0 33 5198.5 34 6678.0 35 8568.5 36 10679.0 37 12818.5 38 15332.0 39 18521.5 40 21883.5 41 25467.0 42 29087.5 43 32542.5 44 36056.0 45 39388.5 46 42500.0 47 45187.0 48 47161.5 49 48536.5 50 49417.0 51 49745.0 52 49453.0 53 48421.5 54 46752.0 55 45034.5 56 42940.5 57 40385.0 58 38026.5 59 35651.0 60 33417.0 61 31168.0 62 28856.5 63 26786.0 64 24676.5 65 22375.5 66 19834.0 67 17642.5 68 15510.5 69 13137.5 70 11048.5 71 9297.0 72 7649.5 73 6094.0 74 4741.0 75 3692.0 76 2833.0 77 2027.5 78 1445.5 79 1042.5 80 737.0 81 521.5 82 364.0 83 236.0 84 156.5 85 105.5 86 60.5 87 36.5 88 24.0 89 15.5 90 9.5 91 6.5 92 5.5 93 4.5 94 3.0 95 1.0 96 0.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0021594241124381288 2 0.007095250655153851 3 7.712228972993316E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 1.8759543917968267E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 7381.0 32-33 16445.0 34-35 18167.0 36-37 20274.0 38-39 21776.0 40-41 23550.0 42-43 25242.0 44-45 26194.0 46-47 26938.0 48-49 27566.0 50-51 27769.0 52-53 27750.0 54-55 27556.0 56-57 26991.0 58-59 26143.0 60-61 25659.0 62-63 25244.0 64-65 24005.0 66-67 23204.0 68-69 22277.0 70-71 21269.0 72-73 20506.0 74-75 19403.0 76-77 18265.0 78-79 16973.0 80-81 15975.0 82-83 14994.0 84-85 14259.0 86-87 13259.0 88-89 11981.0 90-91 11306.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.7813529690974 #Duplication Level Percentage of deduplicated Percentage of total 1 99.79108053744108 99.57289030274033 2 0.20029516328513475 0.39971444771514053 3 0.007041917396714952 0.021079561360225263 4 0.0015823818770721813 0.006315688184321687 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0016966903740585296 0.0 0.0 0.0 0.0 2 0.0016966903740585296 0.0 0.0 0.0 0.0 3 0.0016966903740585296 0.0 0.0 0.0 0.0 4 0.001850934953518396 0.0 0.0 0.0 1.5424457945986634E-4 5 0.0020051795329782624 0.0 0.0 0.0 1.5424457945986634E-4 6 0.0021594241124381288 0.0 0.0 0.0 1.5424457945986634E-4 7 0.002776402430277594 1.5424457945986634E-4 0.0 0.0 3.084891589197327E-4 8 0.00925467476759198 1.5424457945986634E-4 0.0 0.0 3.084891589197327E-4 9 0.009563163926511712 1.5424457945986634E-4 0.0 0.0 3.084891589197327E-4 10-11 0.009871653085431446 1.5424457945986634E-4 0.0 0.0 3.084891589197327E-4 12-13 0.009871653085431446 1.5424457945986634E-4 0.0 0.0 4.62733738379599E-4 14-15 0.009871653085431446 1.5424457945986634E-4 0.0 0.0 4.62733738379599E-4 16-17 0.009871653085431446 1.5424457945986634E-4 0.0 0.0 4.62733738379599E-4 18-19 0.009948775375161378 1.5424457945986634E-4 0.0 0.0 4.62733738379599E-4 20-21 0.010025897664891311 1.5424457945986634E-4 0.0 0.0 4.62733738379599E-4 22-23 0.010025897664891311 1.5424457945986634E-4 0.0 1.5424457945986634E-4 4.62733738379599E-4 24-25 0.010025897664891311 1.5424457945986634E-4 0.0 1.5424457945986634E-4 4.62733738379599E-4 26-27 0.010103019954621244 1.5424457945986634E-4 0.0 1.5424457945986634E-4 4.62733738379599E-4 28-29 0.010334386823811045 1.5424457945986634E-4 0.0 1.5424457945986634E-4 5.398560281095322E-4 30-31 0.010411509113540978 1.5424457945986634E-4 0.0 1.5424457945986634E-4 7.712228972993316E-4 32-33 0.01048863140327091 1.5424457945986634E-4 0.0 1.5424457945986634E-4 8.483451870292648E-4 34-35 0.01071999827246071 1.5424457945986634E-4 0.0 1.5424457945986634E-4 9.25467476759198E-4 36-37 0.01095136514165051 1.5424457945986634E-4 0.0 1.5424457945986634E-4 9.25467476759198E-4 38-39 0.01095136514165051 2.313668691897995E-4 0.0 1.5424457945986634E-4 0.0010025897664891312 40-41 0.011105609721110375 3.084891589197327E-4 0.0 1.5424457945986634E-4 0.0010797120562190644 42-43 0.011105609721110375 3.084891589197327E-4 0.0 1.5424457945986634E-4 0.001311078925408864 44-45 0.011414098880030109 3.084891589197327E-4 0.0 1.5424457945986634E-4 0.001388201215138797 46-47 0.011414098880030109 3.084891589197327E-4 0.0 1.5424457945986634E-4 0.001388201215138797 48-49 0.011722588038949842 3.084891589197327E-4 0.0 1.5424457945986634E-4 0.001388201215138797 50-51 0.011953954908139641 3.856114486496658E-4 0.0 1.5424457945986634E-4 0.001388201215138797 52-53 0.012108199487599506 4.62733738379599E-4 0.0 1.5424457945986634E-4 0.001388201215138797 54-55 0.01218532177732944 4.62733738379599E-4 0.0 1.5424457945986634E-4 0.001388201215138797 56-57 0.01241668864651924 4.62733738379599E-4 0.0 1.5424457945986634E-4 0.001388201215138797 58-59 0.01264805551570904 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