##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-37C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1017861 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.58241449470999 40.0 40.0 40.0 40.0 40.0 2 39.319197807952165 40.0 40.0 40.0 40.0 40.0 3 39.301668891921395 40.0 40.0 40.0 40.0 40.0 4 39.20974278413261 40.0 40.0 40.0 40.0 40.0 5 39.228346503107986 40.0 40.0 40.0 40.0 40.0 6 39.331559024267555 40.0 40.0 40.0 40.0 40.0 7 39.3704562803762 40.0 40.0 40.0 40.0 40.0 8 39.372216835108134 40.0 40.0 40.0 40.0 40.0 9 39.389818452617796 40.0 40.0 40.0 40.0 40.0 10-11 39.3967742157328 40.0 40.0 40.0 40.0 40.0 12-13 39.3254992577572 40.0 40.0 40.0 40.0 40.0 14-15 39.35188792968785 40.0 40.0 40.0 40.0 40.0 16-17 39.46139404103311 40.0 40.0 40.0 40.0 40.0 18-19 39.494086127673626 40.0 40.0 40.0 40.0 40.0 20-21 39.47553742603361 40.0 40.0 40.0 40.0 40.0 22-23 39.510113856410655 40.0 40.0 40.0 40.0 40.0 24-25 39.48414763901947 40.0 40.0 40.0 40.0 40.0 26-27 39.45919531252302 40.0 40.0 40.0 40.0 40.0 28-29 39.47707398161438 40.0 40.0 40.0 40.0 40.0 30-31 39.449243368878484 40.0 40.0 40.0 40.0 40.0 32-33 39.45002323511736 40.0 40.0 40.0 40.0 40.0 34-35 39.445105597042705 40.0 40.0 40.0 40.0 40.0 36-37 39.42426440666517 40.0 40.0 40.0 40.0 40.0 38-39 39.36963045114895 40.0 40.0 40.0 40.0 40.0 40-41 39.411047340957 40.0 40.0 40.0 40.0 40.0 42-43 39.38689042899105 40.0 40.0 40.0 40.0 40.0 44-45 39.29215774540733 40.0 40.0 40.0 40.0 40.0 46-47 39.26706251524364 40.0 40.0 40.0 40.0 40.0 48-49 39.34586773246335 40.0 40.0 40.0 40.0 40.0 50-51 39.310353456531026 40.0 40.0 40.0 40.0 40.0 52-53 39.26853086078276 40.0 40.0 40.0 40.0 40.0 54-55 39.17415076418253 40.0 40.0 40.0 40.0 40.0 56-57 39.18655560302203 40.0 40.0 40.0 40.0 40.0 58-59 39.13878301323266 40.0 40.0 40.0 40.0 40.0 60-61 39.05480709588761 40.0 40.0 40.0 40.0 40.0 62-63 39.388953153434336 40.0 40.0 40.0 40.0 40.0 64-65 39.39427931648861 40.0 40.0 40.0 40.0 40.0 66-67 39.397570856233614 40.0 40.0 40.0 40.0 40.0 68-69 39.39481872773048 40.0 40.0 40.0 40.0 40.0 70-71 39.39850337323134 40.0 40.0 40.0 40.0 40.0 72-73 39.38460163837194 40.0 40.0 40.0 40.0 40.0 74-75 39.39604220166052 40.0 40.0 40.0 40.0 40.0 76-77 39.39282158173556 40.0 40.0 40.0 40.0 40.0 78-79 39.385656798324774 40.0 40.0 40.0 40.0 40.0 80-81 39.38112969824766 40.0 40.0 40.0 40.0 40.0 82-83 39.39067312237352 40.0 40.0 40.0 40.0 40.0 84-85 39.348640687619266 40.0 40.0 40.0 40.0 40.0 86-87 39.36176595388328 40.0 40.0 40.0 40.0 40.0 88-89 39.38184639392256 40.0 40.0 40.0 40.0 40.0 90-91 39.43612085655505 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03722293950761468 1101 2 -0.0010961254259171938 1101 3 0.0670079619978523 1101 4 0.026802670447885646 1101 5 0.053644813474825526 1101 6 0.017022084825498496 1101 7 0.08456148006744257 1101 8 0.1701944920113192 1101 9 -0.01384121748445466 1101 10-11 -0.030612046859936015 1101 12-13 0.08905921758005775 1101 14-15 -0.02489997679541034 1101 16-17 0.07374217825866936 1101 18-19 0.06415767215716528 1101 20-21 0.040859625615951245 1101 22-23 0.06786870936512202 1101 24-25 0.041440915030676706 1101 26-27 0.08957319132209562 1101 28-29 0.02600485179833356 1101 30-31 0.07084239719058871 1101 32-33 0.06240135507040634 1101 34-35 0.1067862573855507 1101 36-37 -0.05113629946861664 1101 38-39 0.015256526961863415 1101 40-41 -0.017936425889374163 1101 42-43 -0.0316373663851266 1101 44-45 0.020707074149541427 1101 46-47 0.020426301918789136 1101 48-49 -0.0195373074199523 1101 50-51 0.07290159757785375 1101 52-53 0.06776083976024694 1101 54-55 -0.03208647632678918 1101 56-57 0.02938771452292599 1101 58-59 0.05282960141278181 1101 60-61 -0.07303731177650974 1101 62-63 -0.09261384862001876 1101 64-65 0.0018401206847329377 1101 66-67 -0.026665547006416546 1101 68-69 -0.11452723864165648 1101 70-71 -0.016222864322600117 1101 72-73 0.025717973952055218 1101 74-75 0.048537094522593804 1101 76-77 -0.10665601038520123 1101 78-79 -0.005119856997552574 1101 80-81 0.043540387180229345 1101 82-83 0.006747642927535935 1101 84-85 -0.17024544945329012 1101 86-87 -0.3557747648920042 1101 88-89 -0.38631096888530436 1101 90-91 -0.36471932923249994 1102 1 0.01098100567580218 1102 2 0.04605928386114755 1102 3 0.09124320686645149 1102 4 -0.13871398738626795 1102 5 -0.06286605943041934 1102 6 -0.07714533520309175 1102 7 -0.2258046315607558 1102 8 -0.07536171175711814 1102 9 -0.17208552050963277 1102 10-11 -0.1601405684743824 1102 12-13 -0.15321039840576134 1102 14-15 -0.08235915051696452 1102 16-17 -0.011327004859488454 1102 18-19 -0.056932156788846555 1102 20-21 -0.06942810668301291 1102 22-23 -0.05264574405039468 1102 24-25 -0.07946187233422108 1102 26-27 -0.04351312836758581 1102 28-29 -0.07424320096520631 1102 30-31 -0.04406526216215667 1102 32-33 0.03838696061177416 1102 34-35 -0.09406148514828061 1102 36-37 -0.04441917297447162 1102 38-39 -0.045949971273785195 1102 40-41 -0.15333646416110724 1102 42-43 -0.045865583365610973 1102 44-45 -0.0948301065512922 1102 46-47 -0.11191460705982337 1102 48-49 -0.09626522931219483 1102 50-51 -0.03530764388088414 1102 52-53 -0.06423170528557165 1102 54-55 -0.11615678944578889 1102 56-57 -0.30492477586978595 1102 58-59 -0.30524748120745926 1102 60-61 -0.28301849513187705 1102 62-63 -0.12922350384552317 1102 64-65 -0.006900631483134134 1102 66-67 0.011756080441585937 1102 68-69 0.01545144584020619 1102 70-71 0.060491683302373644 1102 72-73 -0.03225996676982845 1102 74-75 -0.20560524574959516 1102 76-77 0.11427032911591084 1102 78-79 0.041343952416305285 1102 80-81 -0.05638925784695914 1102 82-83 -0.04163505978618076 1102 84-85 0.1887967376628339 1102 86-87 0.30249620388155307 1102 88-89 0.04919627749150379 1102 90-91 0.2929837519999978 1103 1 0.032362499220866425 1103 2 -0.03849640439060664 1103 3 -0.05637754642745563 1103 4 -0.10547080336989012 1103 5 0.025131173604542312 1103 6 -0.008273955953740142 1103 7 -0.06226678864938151 1103 8 -0.010794793710857675 1103 9 -0.08449488348281875 1103 10-11 0.028501057628027127 1103 12-13 -0.036141550788613586 1103 14-15 0.050497415260501555 1103 16-17 0.013131241162781748 1103 18-19 0.021217004100833492 1103 20-21 -0.0691147989364751 1103 22-23 -0.03478720249795941 1103 24-25 -0.026798888500373153 1103 26-27 0.028094413196292578 1103 28-29 -0.0481436095853951 1103 30-31 0.06804346618682189 1103 32-33 -0.0019246449735916826 1103 34-35 0.014474216486391356 1103 36-37 0.0012082529452754898 1103 38-39 0.020493683516392025 1103 40-41 -0.00516574341993703 1103 42-43 0.02558813507778268 1103 44-45 -0.008890740334713598 1103 46-47 -0.04040958839281927 1103 48-49 -0.017798882102695757 1103 50-51 -0.016731372875284478 1103 52-53 -0.01954711763180228 1103 54-55 -0.004735499284500122 1103 56-57 0.011319358919955391 1103 58-59 0.008098544452948886 1103 60-61 0.07056527305945082 1103 62-63 -0.05847555646779057 1103 64-65 -0.05116324237276615 1103 66-67 -0.038573921121709986 1103 68-69 -0.24716516583673354 1103 70-71 -0.3289662691025441 1103 72-73 -0.046424073750259254 1103 74-75 -0.13002686981914024 1103 76-77 -0.01686474711330277 1103 78-79 -0.24340472306489858 1103 80-81 -0.3479296834346286 1103 82-83 -0.024103657258827127 1103 84-85 0.098086710837471 1103 86-87 -0.272865733708727 1103 88-89 -0.16597856751842244 1103 90-91 -0.285871045733046 1104 1 0.05902663564759081 1104 2 0.002859154327751412 1104 3 0.033692496593118904 1104 4 0.0068107603443934295 1104 5 -0.04744425321639767 1104 6 0.0418473244256532 1104 7 -0.03429247318435813 1104 8 0.02324733175134952 1104 9 -0.07361831781561534 1104 10-11 0.04174539063793503 1104 12-13 -0.0630795077021844 1104 14-15 -0.03901827125230284 1104 16-17 -0.0029169356455369666 1104 18-19 -0.06195986811966492 1104 20-21 -0.011197433366845644 1104 22-23 0.033792048278066034 1104 24-25 0.039364069783381694 1104 26-27 0.032887571496807766 1104 28-29 0.06332624102429918 1104 30-31 0.03469174095528871 1104 32-33 0.03609837704273389 1104 34-35 -0.011968362045152503 1104 36-37 -0.06264034554860132 1104 38-39 0.01800865042217481 1104 40-41 -0.062125856633386434 1104 42-43 0.003186493741054619 1104 44-45 -0.030443931783047162 1104 46-47 -0.025086008176025132 1104 48-49 -0.08462014403770013 1104 50-51 -0.031422855069337174 1104 52-53 -0.08143436438898277 1104 54-55 -0.1456056924560798 1104 56-57 -0.02211892290929285 1104 58-59 -0.0703442104434373 1104 60-61 -0.05774167791536655 1104 62-63 -0.03145963250831585 1104 64-65 -0.02875048422518489 1104 66-67 -0.13871763830133688 1104 68-69 -0.19236150264951846 1104 70-71 -0.22898407074108462 1104 72-73 -0.2606238744406042 1104 74-75 -0.11912826830996437 1104 76-77 -0.11820910547457686 1104 78-79 0.12253402394178181 1104 80-81 -0.1605441721025045 1104 82-83 0.1093015786307916 1104 84-85 -0.06465594561208121 1104 86-87 0.36860646160471333 1104 88-89 0.2929901063835132 1104 90-91 0.18565661061713 1201 1 0.048538271176930436 1201 2 0.05923411335757578 1201 3 0.05335817997561776 1201 4 0.12349381508054336 1201 5 0.05743939149025579 1201 6 0.1650856069559694 1201 7 0.13745449502278717 1201 8 -0.13145604419096912 1201 9 0.011599643508539259 1201 10-11 -0.012663000053642293 1201 12-13 -0.021126049819521597 1201 14-15 0.050132826748352954 1201 16-17 -0.023893664586864816 1201 18-19 0.02129152486624264 1201 20-21 0.06484320904547758 1201 22-23 0.05411325443997583 1201 24-25 0.024942568636213025 1201 26-27 0.055830868776389764 1201 28-29 0.05283787655363881 1201 30-31 0.08582251774977578 1201 32-33 0.0024837829270012435 1201 34-35 0.05822318625536127 1201 36-37 0.06448230251476161 1201 38-39 0.13699049796632323 1201 40-41 0.14172327667779427 1201 42-43 0.12731747734320464 1201 44-45 0.08289358529660973 1201 46-47 0.09250026331660166 1201 48-49 0.10943826774767729 1201 50-51 -0.11472944796760487 1201 52-53 -0.04429112053045969 1201 54-55 0.07865116266027172 1201 56-57 -0.017805009108499803 1201 58-59 -0.07759888898466727 1201 60-61 -0.014257127721300833 1201 62-63 -0.01279886322666357 1201 64-65 -0.05951394133775523 1201 66-67 -0.058405482563486544 1201 68-69 -0.05195079607893405 1201 70-71 0.1383429570942738 1201 72-73 0.10842854487111708 1201 74-75 0.2305067456161467 1201 76-77 0.11420770878230257 1201 78-79 0.1903866764254758 1201 80-81 0.004353287970133124 1201 82-83 0.2611078300928398 1201 84-85 0.20986217994052225 1201 86-87 0.16563777717261274 1201 88-89 0.117507539650191 1201 90-91 -0.16989894493842428 1202 1 0.11609905179440005 1202 2 0.12241069157429507 1202 3 0.07926404042965629 1202 4 -0.003424259890010717 1202 5 -0.02216284502222976 1202 6 0.07058212309249257 1202 7 0.16635572738171334 1202 8 0.06023254837642611 1202 9 0.11100184019426962 1202 10-11 0.07185967691713557 1202 12-13 0.07641647849729338 1202 14-15 -0.00762639997376624 1202 16-17 0.006935243641791544 1202 18-19 -0.004769688185504606 1202 20-21 -0.02844330992324018 1202 22-23 -0.01515336288322544 1202 24-25 0.02590553746563984 1202 26-27 0.022610616924843896 1202 28-29 -0.053139971904144545 1202 30-31 0.021504586811715853 1202 32-33 0.014126563300386863 1202 34-35 0.03763895412591012 1202 36-37 -0.16361618812358358 1202 38-39 -0.11821183347720421 1202 40-41 -0.102088083553177 1202 42-43 -0.08303195615152958 1202 44-45 -0.028790256854613006 1202 46-47 -0.024455121450586148 1202 48-49 0.07016762380344232 1202 50-51 0.0760305221575166 1202 52-53 0.02233525702828132 1202 54-55 0.09603670917731222 1202 56-57 -0.08457802738810472 1202 58-59 -0.09140256805915214 1202 60-61 -0.11442643457159107 1202 62-63 -0.006237931439507349 1202 64-65 -0.00333712094338523 1202 66-67 -0.03386075030961422 1202 68-69 -0.13925006114503446 1202 70-71 -0.1317133330525806 1202 72-73 -0.16456587851514115 1202 74-75 -0.2974590416042844 1202 76-77 -0.049711039033141446 1202 78-79 -0.08731305788168697 1202 80-81 -0.1439839415505162 1202 82-83 -0.5760822359335762 1202 84-85 -0.693680338457419 1202 86-87 -0.45682012569687913 1202 88-89 -0.5544844848670323 1202 90-91 0.1949445363137272 1203 1 0.10027117684436604 1203 2 -0.015422147287033283 1203 3 0.014056405014208906 1203 4 0.0018200383397655173 1203 5 0.1255661563438153 1203 6 -0.032814082619573526 1203 7 0.10047889314319747 1203 8 -0.04754012222455373 1203 9 0.027153352792872454 1203 10-11 0.017989605359922223 1203 12-13 -0.03742148409371282 1203 14-15 0.0028888690247441673 1203 16-17 -0.03326526012223496 1203 18-19 -0.039901108447253364 1203 20-21 0.025826416018588816 1203 22-23 -0.019605074690993263 1203 24-25 0.040976823078764824 1203 26-27 0.00900827260382897 1203 28-29 -0.02149094435129939 1203 30-31 0.008798855561295227 1203 32-33 -0.022992339097854142 1203 34-35 -0.09598195546619337 1203 36-37 0.033222024128860994 1203 38-39 0.039024349512274625 1203 40-41 -0.034471372796929245 1203 42-43 0.0759764262299143 1203 44-45 -6.787555855183314E-4 1203 46-47 0.03098330336194266 1203 48-49 0.04000703034769515 1203 50-51 0.05420592739989161 1203 52-53 -0.013594570026519648 1203 54-55 -0.07518136351917093 1203 56-57 0.06949746291955705 1203 58-59 -0.09581487939817634 1203 60-61 0.04491361360427959 1203 62-63 0.11971102095044017 1203 64-65 -0.0012504463496583185 1203 66-67 -0.033366763999353566 1203 68-69 0.02065488382116598 1203 70-71 0.19069213677317265 1203 72-73 0.05697704144918703 1203 74-75 0.10852294702240073 1203 76-77 -0.008096697323317414 1203 78-79 0.021966726524510705 1203 80-81 -0.1870992258450599 1203 82-83 -0.06497198628142087 1203 84-85 -0.40492628298152766 1203 86-87 -0.044592537637456076 1203 88-89 -0.0035270861014140564 1203 90-91 -0.7894446039577758 1204 1 -0.039294091848127266 1204 2 -0.09087099135786048 1204 3 -0.33518420457019005 1204 4 -0.2974479270144954 1204 5 -0.1325930097896446 1204 6 -0.1499805556694227 1204 7 -0.22067624620814996 1204 8 -0.1726444051052738 1204 9 -0.20780632982263825 1204 10-11 -0.1628373095199862 1204 12-13 -0.13433733288520955 1204 14-15 -0.1171119879415059 1204 16-17 -0.084482424005202 1204 18-19 -0.06903028683724699 1204 20-21 -0.12147904864417569 1204 22-23 -0.1401098789781301 1204 24-25 -0.10961261302145431 1204 26-27 -0.06584069508758006 1204 28-29 -0.08195183231543268 1204 30-31 -0.07237377575910386 1204 32-33 -0.09032766238095036 1204 34-35 -0.044650697476342316 1204 36-37 -0.047199212303056015 1204 38-39 -0.07445479966453661 1204 40-41 -0.08438112202485115 1204 42-43 -0.10099099743615625 1204 44-45 -0.09383737955291593 1204 46-47 -0.07208589245825436 1204 48-49 0.0075779449863730974 1204 50-51 -0.09134759003594439 1204 52-53 -0.1461427114070375 1204 54-55 -0.057078243595100275 1204 56-57 -0.05805110777727407 1204 58-59 0.059275929656998017 1204 60-61 -0.057485900936526946 1204 62-63 -0.1400064587116816 1204 64-65 -0.07279449302671992 1204 66-67 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28-29 0.06835451153929739 2401 30-31 0.022010383141186196 2401 32-33 0.06826935661848665 2401 34-35 0.018897062785910634 2401 36-37 0.027153479913906153 2401 38-39 0.12960295459615168 2401 40-41 0.09947743094754458 2401 42-43 0.02903107491827228 2401 44-45 0.13772509527759524 2401 46-47 0.07643961573252511 2401 48-49 0.010965668404587348 2401 50-51 0.09441733348369752 2401 52-53 -0.048570766871328885 2401 54-55 0.09519987503433924 2401 56-57 0.1036919557454965 2401 58-59 0.05872611872609923 2401 60-61 0.11439562723327157 2401 62-63 0.135677011164546 2401 64-65 0.029992168425494015 2401 66-67 -0.06061366145840452 2401 68-69 0.12279749932581296 2401 70-71 0.031802394659372624 2401 72-73 -0.012634806780127406 2401 74-75 0.18878853740795165 2401 76-77 0.13235092332173792 2401 78-79 0.07599986814332027 2401 80-81 0.22392808864356084 2401 82-83 0.21575387962136006 2401 84-85 0.24801821705917604 2401 86-87 0.25819702101122743 2401 88-89 0.508988176413844 2401 90-91 0.22575686124369554 2402 1 -0.01947764943116681 2402 2 -0.02545639153245105 2402 3 -0.007074905407044696 2402 4 0.09687255235598968 2402 5 -0.05684924563096416 2402 6 -0.004984192086588735 2402 7 0.02156906671345382 2402 8 -5.237817691821078E-4 2402 9 -0.055376362992461736 2402 10-11 0.042797803712666393 2402 12-13 0.04801343183291351 2402 14-15 0.06284081557889465 2402 16-17 0.057052341892017466 2402 18-19 0.03509884597308144 2402 20-21 0.019853848070582103 2402 22-23 0.03583899459278683 2402 24-25 0.03608876338800826 2402 26-27 0.010904194931470101 2402 28-29 0.015783629399628296 2402 30-31 -0.020669840237189874 2402 32-33 0.024769778753373828 2402 34-35 -0.017900674851425435 2402 36-37 -0.004917212449186081 2402 38-39 -0.25600511890566224 2402 40-41 0.03054543635273177 2402 42-43 0.04068896387629195 2402 44-45 -0.022223742185282447 2402 46-47 0.07743884110421817 2402 48-49 0.041881923192185866 2402 50-51 0.0716066489742957 2402 52-53 0.004499001803893066 2402 54-55 -0.038630320454636546 2402 56-57 0.05930993226716197 2402 58-59 0.025520237314871963 2402 60-61 0.09364143511974277 2402 62-63 -0.05517253336913086 2402 64-65 0.10997906799896384 2402 66-67 0.03352783995310915 2402 68-69 0.10599115175551077 2402 70-71 -0.10080201333801853 2402 72-73 0.005147015180313019 2402 74-75 0.001901722258857319 2402 76-77 0.0377101572084797 2402 78-79 0.008395250538697496 2402 80-81 0.09169772638837514 2402 82-83 0.11448737472844783 2402 84-85 0.13484929436095427 2402 86-87 0.3478121418699942 2402 88-89 0.45086851829418606 2402 90-91 0.6067092421960751 2403 1 -0.10395990229124408 2403 2 0.01971814645522585 2403 3 0.046899867253564764 2403 4 0.12385993868629441 2403 5 0.06048721667470147 2403 6 0.025523288112886178 2403 7 0.055596640782098916 2403 8 0.005343041346101529 2403 9 0.0537465469517997 2403 10-11 0.09090575325798511 2403 12-13 0.11372185072407603 2403 14-15 0.1731370901462128 2403 16-17 -0.012762853179850708 2403 18-19 0.08438016014146399 2403 20-21 -0.026494054540151524 2403 22-23 -0.001121991033492975 2403 24-25 0.06835629052206826 2403 26-27 0.10653341171058628 2403 28-29 0.07973200135621283 2403 30-31 0.10488017442986575 2403 32-33 0.10209094107652561 2403 34-35 0.02657781403946302 2403 36-37 0.04710787125758742 2403 38-39 0.0758878540518566 2403 40-41 -0.08127545571169037 2403 42-43 0.03247003935651804 2403 44-45 0.05591393196613126 2403 46-47 0.010695895181363824 2403 48-49 0.07563456208294639 2403 50-51 0.0520773839015618 2403 52-53 0.15121847179869263 2403 54-55 -0.028447825108393943 2403 56-57 0.11955872028143233 2403 58-59 0.0313584697769258 2403 60-61 0.17553671667649695 2403 62-63 0.0458228155282967 2403 64-65 0.1779726601935181 2403 66-67 -0.00891770703243111 2403 68-69 0.062005595559654125 2403 70-71 -0.22525903708343264 2403 72-73 0.020120738666477678 2403 74-75 0.00755043385117915 2403 76-77 0.0812821594580555 2403 78-79 -0.0435858608851305 2403 80-81 0.009818252006525086 2403 82-83 0.11677443455982939 2403 84-85 -0.21901397320985438 2403 86-87 0.050921108982649343 2403 88-89 0.32556586395926956 2403 90-91 0.2924235279103584 2404 1 0.003879103706196929 2404 2 -0.08969690313041667 2404 3 -0.02745945523650306 2404 4 -0.04288622818242516 2404 5 0.04245557530820321 2404 6 -0.09098138294594804 2404 7 -0.017783962415890642 2404 8 0.08069859090934273 2404 9 0.1254089845738946 2404 10-11 0.05656561380335745 2404 12-13 0.052593604682769524 2404 14-15 0.0883669887675822 2404 16-17 0.042511099824807275 2404 18-19 -0.01631223827178019 2404 20-21 0.021168476456765006 2404 22-23 0.07742151949288711 2404 24-25 0.0613739471193 2404 26-27 0.06377117132566212 2404 28-29 0.09375281604819463 2404 30-31 0.08383753800520566 2404 32-33 -0.0058879987396665 2404 34-35 0.06244448715382589 2404 36-37 0.05729527961261027 2404 38-39 0.09613211835859659 2404 40-41 0.04192160401579059 2404 42-43 0.03688173228075442 2404 44-45 0.04833734172451187 2404 46-47 0.09311839106585751 2404 48-49 0.07245033667069833 2404 50-51 0.023128236611611896 2404 52-53 0.08174817283330071 2404 54-55 -0.05331365648137165 2404 56-57 0.12330780345806858 2404 58-59 -0.009321494022486831 2404 60-61 -0.029458518488610252 2404 62-63 0.07852617386562599 2404 64-65 0.07054856359109607 2404 66-67 -0.029195363046838452 2404 68-69 0.18769956926313114 2404 70-71 0.11994040960956198 2404 72-73 0.20917060585556158 2404 74-75 0.1664230485688094 2404 76-77 0.08321692267067249 2404 78-79 0.12861738924348032 2404 80-81 0.11717465367063795 2404 82-83 0.0975536117332183 2404 84-85 0.05785045440165959 2404 86-87 0.05761439811264779 2404 88-89 0.21697921450196844 2404 90-91 0.43279619871780994 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 1.0 30 37.0 31 666.0 32 4360.0 33 7940.0 34 10589.0 35 13044.0 36 18724.0 37 28192.0 38 70576.0 39 420736.0 40 442996.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 31.760819994085637 14.267075759853261 23.40083763893105 30.57126660713005 2 29.831287891442553 19.546629196922503 26.28980750901534 24.332275402619608 3 27.144472575331996 20.239109269340315 21.865853982027016 30.750564173300678 4 26.62740786806843 20.665493618480323 22.40757824496665 30.2995202684846 5 26.91890150030309 22.04446383150548 22.66743690936189 28.369197758829547 6 27.79593677329223 24.48733176730418 23.574535226322652 24.14219623308094 7 27.377706779216414 23.9494390687923 22.844769570697768 25.828084581293513 8 26.122132589813347 25.03662091385759 23.27439601281511 25.56685048351396 9 25.34127940848505 24.78904290467952 24.239459022400897 25.630218664434533 10-11 25.621573083161646 23.99733362413925 23.939761912481174 26.441331380217925 12-13 25.641909848201276 23.60734913706292 23.952091690319207 26.798649324416594 14-15 25.756512922687875 23.546338841944035 23.968646013551947 26.728502221816143 16-17 26.209816468063913 23.356971138495336 23.467742648554175 26.965469744886583 18-19 26.15239212426844 23.47127947725672 23.487047838555558 26.889280559919282 20-21 25.9121333855998 23.68727164121624 23.66727873452269 26.73331623866127 22-23 26.06082755896925 23.093182664430607 23.593103577011007 27.25288619958914 24-25 26.004434790212024 23.36645180432299 23.651019147015163 26.978094258449826 26-27 26.051150402658124 23.691054083023126 23.56903349278536 26.688762021533392 28-29 26.274854818094024 23.33324491261577 23.439546264175558 26.95235400511465 30-31 26.113104963300128 23.459180174701405 23.49671022575996 26.93100463623851 32-33 26.07379712467174 23.576351432788066 23.599806557732865 26.750044884807327 34-35 26.28534314073644 23.26314370089479 23.47096430199967 26.980548856369097 36-37 26.264618079772134 23.398198384438782 23.469380687324808 26.86780284846428 38-39 26.367767824229315 23.554786215840505 23.489776022952874 26.58766993697731 40-41 26.321202853637693 23.393724090214025 23.408259734520993 26.876813321627296 42-43 26.276642657651035 23.44902983977657 23.51738203733647 26.756945465235926 44-45 26.18805210166647 23.64379642323071 23.597300238252416 26.5708512368504 46-47 26.39558192028758 23.251055943423392 23.5094401376576 26.84392199863143 48-49 26.363731059136935 23.463362344408072 23.45191157643092 26.72099502002407 50-51 26.358472300863944 23.64156740603561 23.530546914893023 26.469413378207424 52-53 26.412901089417325 23.4284346585523 23.412907094552573 26.745757157477808 54-55 26.45080830549015 23.50563533649148 23.47008292460469 26.573473433413685 56-57 26.434075038586112 23.62889993570671 23.579615173216673 26.357409852490505 58-59 26.410163073515147 23.41570983701527 23.450224511855957 26.72390257761363 60-61 26.592056236268537 23.52988589831113 23.366115840753466 26.511942024666872 62-63 26.443123708712697 23.59158175577114 23.530847192924423 26.43444734259174 64-65 26.697568205190247 23.422206763051236 23.378500998124736 26.501724033633778 66-67 26.670774537312873 23.39211662278714 23.396530037531 26.540578802368987 68-69 26.585596779738164 23.539138434001725 23.49170256364953 26.383562222610585 70-71 26.77344685845931 23.187901164815095 23.42660175762473 26.612050219100862 72-73 26.724011768446232 23.372124123800823 23.32594861650106 26.577915491251886 74-75 26.63499334095767 23.4998044916931 23.497158514607264 26.368043652741967 76-77 26.85428392745466 23.266277534570218 23.315613925370023 26.563824612605103 78-79 26.93311289521257 23.212802110862004 23.17814990627205 26.67593508765338 80-81 26.96528900177018 23.127324969855565 23.37515072218374 26.53223530619051 82-83 26.985016286644953 22.90358306188925 22.86970684039088 27.241693811074917 84-85 27.327035287579882 22.78150180605724 22.646915809947206 27.244547096415673 86-87 27.565960516986156 22.49685902300066 22.539153366754157 27.398027093259024 88-89 26.997667853197495 21.367783642240497 22.932776891289226 28.70177161327278 90-91 22.62065516379095 24.426106526631656 25.756439109777446 27.196799199799948 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.0 4 0.5 5 1.0 6 2.0 7 3.5 8 5.0 9 4.5 10 9.0 11 20.5 12 27.5 13 32.5 14 43.0 15 64.0 16 97.0 17 151.5 18 230.5 19 329.0 20 466.5 21 643.5 22 836.5 23 1116.0 24 1485.5 25 1874.5 26 2426.0 27 3167.0 28 3989.5 29 4902.0 30 5976.5 31 7348.0 32 8985.0 33 10616.0 34 12682.5 35 15167.0 36 17939.5 37 20792.0 38 24037.5 39 27915.5 40 31946.0 41 36323.0 42 40541.0 43 44688.0 44 48987.5 45 53058.5 46 56830.0 47 60385.0 48 63253.5 49 65561.0 50 67936.0 51 69437.0 52 69841.0 53 70010.0 54 69867.5 55 69526.0 56 69032.0 57 68217.0 58 66869.0 59 64845.0 60 62568.0 61 59791.0 62 56835.0 63 53367.0 64 49049.5 65 44668.0 66 39827.5 67 35343.5 68 31236.5 69 26776.0 70 22453.5 71 18764.0 72 15591.5 73 12495.0 74 9777.0 75 7689.5 76 5974.5 77 4449.5 78 3314.0 79 2434.0 80 1701.5 81 1174.0 82 837.5 83 596.0 84 402.5 85 274.0 86 173.5 87 114.0 88 80.5 89 51.0 90 35.0 91 25.0 92 17.5 93 12.5 94 8.0 95 4.0 96 1.0 97 0.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014835031502336764 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.05493145774772146 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 1.3556803685281515E-4 64-65 1.5123079179905663E-4 66-67 0.0 68-69 0.0 70-71 2.1899088997897687E-4 72-73 2.523245398231205E-4 74-75 0.0 76-77 3.4743816469263886E-4 78-79 4.174946978173377E-4 80-81 0.0 82-83 6.51461553996391E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 11700.0 32-33 27090.0 34-35 31787.0 36-37 36392.0 38-39 40388.0 40-41 43078.0 42-43 44752.0 44-45 46927.0 46-47 47015.0 48-49 47363.0 50-51 47122.0 52-53 45778.0 54-55 44690.0 56-57 42892.0 58-59 41298.0 60-61 40898.0 62-63 38886.0 64-65 36507.0 66-67 34765.0 68-69 32436.0 70-71 30611.0 72-73 28666.0 74-75 26598.0 76-77 24659.0 78-79 22620.0 80-81 21224.0 82-83 19560.0 84-85 17821.0 86-87 16131.0 88-89 14704.0 90-91 13503.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.92369789397858 #Duplication Level Percentage of deduplicated Percentage of total 1 99.927380745949 99.8511340499478 2 0.06945858502399839 0.13881117332162476 3 0.002580221512651796 0.007734758247892874 4 5.804475143453448E-4 0.002320018482670201 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002456131043433239 0.0 0.0 0.0 9.824524173732955E-5 2 0.002456131043433239 0.0 0.0 0.0 9.824524173732955E-5 3 0.002456131043433239 0.0 0.0 0.0 9.824524173732955E-5 4 0.002652621526907898 0.0 0.0 0.0 9.824524173732955E-5 5 0.0027508667686452276 0.0 0.0 0.0 9.824524173732955E-5 6 0.0031438477355945457 0.0 0.0 0.0 9.824524173732955E-5 7 0.0036350739442811936 9.824524173732955E-5 0.0 9.824524173732955E-5 9.824524173732955E-5 8 0.01110171231631824 9.824524173732955E-5 0.0 9.824524173732955E-5 9.824524173732955E-5 9 0.011494693283267559 9.824524173732955E-5 0.0 9.824524173732955E-5 9.824524173732955E-5 10-11 0.011494693283267559 9.824524173732955E-5 0.0 9.824524173732955E-5 9.824524173732955E-5 12-13 0.011543815904136223 9.824524173732955E-5 0.0 9.824524173732955E-5 9.824524173732955E-5 14-15 0.011592938525004887 9.824524173732955E-5 0.0 9.824524173732955E-5 9.824524173732955E-5 16-17 0.011642061145873552 9.824524173732955E-5 4.912262086866478E-5 9.824524173732955E-5 9.824524173732955E-5 18-19 0.011789429008479548 1.964904834746591E-4 9.824524173732955E-5 9.824524173732955E-5 9.824524173732955E-5 20-21 0.01193679687108554 1.964904834746591E-4 9.824524173732955E-5 9.824524173732955E-5 1.4736786260599434E-4 22-23 0.012035042112822872 1.964904834746591E-4 9.824524173732955E-5 9.824524173732955E-5 2.456131043433239E-4 24-25 0.012280655217166194 1.964904834746591E-4 9.824524173732955E-5 9.824524173732955E-5 2.947357252119887E-4 26-27 0.01232977783803486 1.964904834746591E-4 1.964904834746591E-4 9.824524173732955E-5 3.4385834608065345E-4 28-29 0.012477145700640853 2.947357252119887E-4 1.964904834746591E-4 9.824524173732955E-5 3.929809669493182E-4 30-31 0.012821004046721507 2.947357252119887E-4 1.964904834746591E-4 9.824524173732955E-5 3.929809669493182E-4 32-33 0.012919249288458837 2.947357252119887E-4 1.964904834746591E-4 9.824524173732955E-5 3.929809669493182E-4 34-35 0.012968371909327502 2.947357252119887E-4 1.964904834746591E-4 9.824524173732955E-5 3.929809669493182E-4 36-37 0.012968371909327502 2.947357252119887E-4 1.964904834746591E-4 9.824524173732955E-5 4.42103587817983E-4 38-39 0.013115739771933496 2.947357252119887E-4 1.964904834746591E-4 9.824524173732955E-5 4.912262086866478E-4 40-41 0.01345959811801415 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