##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-37B_S51_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1165759
Sequences flagged as poor quality	0
Sequence length	61
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.49877461808144	40.0	40.0	40.0	40.0	40.0
2	39.18183432424712	40.0	40.0	40.0	40.0	40.0
3	39.174901501939935	40.0	40.0	40.0	40.0	40.0
4	39.09379897560302	40.0	40.0	40.0	40.0	40.0
5	39.0938470129761	40.0	40.0	40.0	40.0	40.0
6	39.202716856571556	40.0	40.0	40.0	40.0	40.0
7	39.2525590623791	40.0	40.0	40.0	40.0	40.0
8	39.23779443264002	40.0	40.0	40.0	40.0	40.0
9	39.250376793145065	40.0	40.0	40.0	40.0	40.0
10	39.253917833788975	40.0	40.0	40.0	40.0	40.0
11	39.2555442419917	40.0	40.0	40.0	40.0	40.0
12	39.25928429460978	40.0	40.0	40.0	40.0	40.0
13	39.08748549228443	40.0	40.0	40.0	40.0	40.0
14	39.252802680485416	40.0	40.0	40.0	40.0	40.0
15	39.16558053594268	40.0	40.0	40.0	40.0	40.0
16	39.349052419925556	40.0	40.0	40.0	40.0	40.0
17	39.3011248465592	40.0	40.0	40.0	40.0	40.0
18	39.37435439057301	40.0	40.0	40.0	40.0	40.0
19	39.32160592369435	40.0	40.0	40.0	40.0	40.0
20	39.333378511338964	40.0	40.0	40.0	40.0	40.0
21	39.310687715042306	40.0	40.0	40.0	40.0	40.0
22	39.37005504568268	40.0	40.0	40.0	40.0	40.0
23	39.36659292358026	40.0	40.0	40.0	40.0	40.0
24	39.360797557642705	40.0	40.0	40.0	40.0	40.0
25	39.32929190338655	40.0	40.0	40.0	40.0	40.0
26	39.29336681080738	40.0	40.0	40.0	40.0	40.0
27	39.31438316152824	40.0	40.0	40.0	40.0	40.0
28	39.31464736708016	40.0	40.0	40.0	40.0	40.0
29	39.31950600424273	40.0	40.0	40.0	40.0	40.0
30	39.31661003689442	40.0	40.0	40.0	40.0	40.0
31	39.26641098202973	40.0	40.0	40.0	40.0	40.0
32	39.288768947955795	40.0	40.0	40.0	40.0	40.0
33	39.299567063175154	40.0	40.0	40.0	40.0	40.0
34	39.28585239316188	40.0	40.0	40.0	40.0	40.0
35	39.29609121610899	40.0	40.0	40.0	40.0	40.0
36	39.256830957341954	40.0	40.0	40.0	40.0	40.0
37	39.26235525524572	40.0	40.0	40.0	40.0	40.0
38	39.24532257524926	40.0	40.0	40.0	40.0	40.0
39	37.994908038453914	40.0	40.0	40.0	40.0	40.0
40	39.23923898507324	40.0	40.0	40.0	40.0	40.0
41	39.2552217053439	40.0	40.0	40.0	40.0	40.0
42	39.205039806683885	40.0	40.0	40.0	40.0	40.0
43	39.238995366966925	40.0	40.0	40.0	40.0	40.0
44	39.194927939651336	40.0	40.0	40.0	40.0	40.0
45	39.0258947175188	40.0	40.0	40.0	40.0	40.0
46	39.0505962210028	40.0	40.0	40.0	40.0	40.0
47	39.095607239575244	40.0	40.0	40.0	40.0	40.0
48	39.20424719002813	40.0	40.0	40.0	40.0	40.0
49	39.195864668426324	40.0	40.0	40.0	40.0	40.0
50	39.172477330220055	40.0	40.0	40.0	40.0	40.0
51	39.12488258722429	40.0	40.0	40.0	40.0	40.0
52	39.13598265164584	40.0	40.0	40.0	40.0	40.0
53	39.039118720078505	40.0	40.0	40.0	40.0	40.0
54	38.92226781007052	40.0	40.0	40.0	40.0	40.0
55	38.98896084010503	40.0	40.0	40.0	40.0	40.0
56	38.98553989289381	40.0	40.0	40.0	40.0	40.0
57	38.88315509466365	40.0	40.0	40.0	40.0	40.0
58	38.87259373506874	40.0	40.0	40.0	40.0	40.0
59	38.8485235799166	40.0	40.0	40.0	40.0	40.0
60	38.778883113919775	40.0	40.0	40.0	40.0	40.0
61	38.67501601960611	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.07828566758694677
1101	2	-0.12128333109262002
1101	3	-0.0876771719505598
1101	4	-0.17860115433964552
1101	5	-0.07572880224777379
1101	6	-0.11335157489755687
1101	7	-0.10142349285151653
1101	8	-0.053242700420632616
1101	9	-0.07300331636457003
1101	10	-0.065369595601382
1101	11	-0.07778202656555067
1101	12	-0.06424405105021691
1101	13	-0.016477048671490024
1101	14	-0.03420041065228929
1101	15	-0.07852643286955896
1101	16	0.010451118019574324
1101	17	-0.04092911865767235
1101	18	0.045818598366302865
1101	19	-0.08712201636910777
1101	20	-0.043020296588018425
1101	21	-0.07407592319403733
1101	22	-0.006029034610392614
1101	23	-0.013840562009548307
1101	24	-0.07234713637974721
1101	25	-0.01640793158626508
1101	26	-0.06489030843879817
1101	27	-0.11786735390267467
1101	28	-0.00829256888135177
1101	29	-0.018623364359569905
1101	30	-0.058304307403417965
1101	31	0.015814208743925917
1101	32	4.0637443794366845E-4
1101	33	0.07328991664258666
1101	34	-0.04695624171457524
1101	35	-0.022891244875879124
1101	36	-0.17569768703513944
1101	37	-0.0968555192905356
1101	38	-0.11115774690639313
1101	39	1.0676753712159766
1101	40	-0.16107667280272153
1101	41	-0.18266310380388262
1101	42	-0.034601076255569296
1101	43	-0.002379503718856313
1101	44	-0.07089878211296963
1101	45	-0.14001434031454352
1101	46	0.051503648372765554
1101	47	-0.015620966857539997
1101	48	-0.06565315064154476
1101	49	-0.08202107187680241
1101	50	-0.08804826123610354
1101	51	-0.09175548784759968
1101	52	-0.12194428565277349
1101	53	-0.058597429738753704
1101	54	-0.09945416158094389
1101	55	-0.08661532888953616
1101	56	0.024575339778891703
1101	57	-0.08654265827782126
1101	58	-0.06535568300131445
1101	59	-0.0595601341590708
1101	60	-0.061167456615990545
1101	61	-0.09021803931652528
1102	1	0.08932262752273346
1102	2	0.10474953046625046
1102	3	0.036737127319149465
1102	4	-0.0578865435976752
1102	5	-0.0310591639154012
1102	6	0.0286651992186151
1102	7	-0.09813122274897523
1102	8	0.03272729053738743
1102	9	0.09137267499087187
1102	10	0.07290339708389837
1102	11	0.035307451456780825
1102	12	0.03143696089954773
1102	13	-0.10241940807685523
1102	14	-0.039707320304167126
1102	15	-0.026380401877538873
1102	16	0.06802939468078506
1102	17	0.08785802079465554
1102	18	-0.05026865619790044
1102	19	0.029242094594508217
1102	20	0.03928351644866268
1102	21	0.043673292834142785
1102	22	0.08222261710976397
1102	23	0.005192286615397279
1102	24	-0.021713785918770157
1102	25	-0.04959283840913997
1102	26	0.030530849335015375
1102	27	0.030640483463891144
1102	28	0.00744624592492471
1102	29	0.07159130340922104
1102	30	0.08341792611965104
1102	31	0.08581139855623121
1102	32	0.04541405836120305
1102	33	0.08698811189066191
1102	34	0.05862215723095687
1102	35	-0.0217248406109789
1102	36	0.02614492599261098
1102	37	0.004043740771756177
1102	38	-4.040169407346639E-4
1102	39	1.2923581080801085
1102	40	0.004796713544003239
1102	41	0.06190729610111134
1102	42	0.08057958118725139
1102	43	-0.021779793203464237
1102	44	0.09817163366546566
1102	45	-0.02137738716943005
1102	46	0.1269360233811483
1102	47	0.13495453371449173
1102	48	0.1220556292549162
1102	49	0.03773760472468268
1102	50	0.06352432685269349
1102	51	0.009768127723425835
1102	52	0.043917342586439645
1102	53	-0.002649414683666862
1102	54	0.14051823038262512
1102	55	0.0815661739612139
1102	56	0.07196462837811879
1102	57	0.115629181760184
1102	58	0.11165204325113365
1102	59	0.003014162382754648
1102	60	0.019354415227695654
1102	61	-0.07129924433441204
1103	1	0.10446027391625279
1103	2	0.05956572125365511
1103	3	0.03827441065024573
1103	4	-0.041123271048540744
1103	5	0.024022143983863486
1103	6	0.0029057316156126944
1103	7	0.004406727732956028
1103	8	-0.004486902697621531
1103	9	-0.0016679786632991522
1103	10	-0.05124102186555035
1103	11	-0.04745588845744919
1103	12	-0.0673767042876463
1103	13	0.0402586854436251
1103	14	-0.050135614590367084
1103	15	-0.02280473344145406
1103	16	-0.04246413738351862
1103	17	-0.07067539377074183
1103	18	-0.15275342370853906
1103	19	-0.019760104225895248
1103	20	-0.09205035383570248
1103	21	0.012812396441070462
1103	22	-0.13238721783984175
1103	23	-0.044143192158387023
1103	24	-0.019821358523870458
1103	25	0.09889106280999016
1103	26	-0.06778585053161379
1103	27	-0.004249613925949802
1103	28	-0.08103751347294974
1103	29	-0.04165681110671926
1103	30	-0.10538900846706412
1103	31	-0.0786616675188796
1103	32	0.03560475476857761
1103	33	-0.04014991742024421
1103	34	-0.07352807166264341
1103	35	-0.09249482957847022
1103	36	-0.02065224774610641
1103	37	-0.10654108872659407
1103	38	-0.05309918219500531
1103	39	1.2291634424503144
1103	40	-0.04271961215087572
1103	41	-0.0643281000027045
1103	42	0.016221390330109386
1103	43	-0.08951451181531667
1103	44	-0.0192277072202387
1103	45	-0.0183235027738462
1103	46	-0.06335885255775509
1103	47	-0.03228404686745989
1103	48	0.08864845038107916
1103	49	0.07116723863083507
1103	50	-0.05396926607657093
1103	51	0.004743814241720656
1103	52	0.002579696311109103
1103	53	-0.05628000931065458
1103	54	-0.022393620024310223
1103	55	0.03408353081481863
1103	56	-0.06162892726397473
1103	57	-0.009653057313819602
1103	58	0.030977612264607046
1103	59	-0.028992531580570358
1103	60	-0.009492028397467323
1103	61	0.046076755136120084
1104	1	0.08102519944860376
1104	2	0.1423107510192736
1104	3	0.03439962868124269
1104	4	-0.11445772141779997
1104	5	-0.09480304922276162
1104	6	0.13148097652452861
1104	7	0.027261428134103483
1104	8	-0.023550853246611325
1104	9	-0.032582783835636064
1104	10	0.012752197411387556
1104	11	-0.053498059350133076
1104	12	-0.058254131160261124
1104	13	-0.004255656534382979
1104	14	0.029990898814610034
1104	15	-0.019981952160115668
1104	16	0.00603635092594601
1104	17	-0.10478196554023356
1104	18	-0.13942168475620065
1104	19	0.05248396400440214
1104	20	-0.06482249413590324
1104	21	-0.11855800673499317
1104	22	-0.005056171643623486
1104	23	-0.12379982355361818
1104	24	-0.06614864253825203
1104	25	-0.08661172511541082
1104	26	-0.05342973390579431
1104	27	0.018437603778657774
1104	28	-0.08283562631223162
1104	29	-0.0145580526521627
1104	30	-0.03280997484367987
1104	31	0.03597558596842987
1104	32	-0.018175800010588716
1104	33	-0.018350997698178162
1104	34	-0.027926255231385255
1104	35	-0.01456361887993296
1104	36	-0.13655434451461446
1104	37	-0.0435164195576192
1104	38	-0.15268305602470633
1104	39	1.1724842157929416
1104	40	-0.16175764795103476
1104	41	-0.07352714526410864
1104	42	-0.05339587463529227
1104	43	-0.09427759176686124
1104	44	0.05234788406673374
1104	45	-0.01831216279959591
1104	46	-0.009434948873391136
1104	47	0.14413076939042924
1104	48	-0.04038359195191532
1104	49	-0.006158010367009581
1104	50	-0.07043316406914357
1104	51	-0.032233515623232734
1104	52	-0.0010717753958360277
1104	53	0.03315109834677088
1104	54	0.09103229159876491
1104	55	0.00389448399417347
1104	56	0.014056096228308945
1104	57	-0.00917416147839134
1104	58	-0.10319579993001327
1104	59	-0.06182204778891531
1104	60	0.002068057655492339
1104	61	0.08688612399022588
1201	1	0.058386820681867846
1201	2	0.02154370958146501
1201	3	0.10414354166324102
1201	4	-0.009951221655065012
1201	5	-0.0899971139012763
1201	6	-0.0017506141967515987
1201	7	-0.0201322089232292
1201	8	-0.009549227811248784
1201	9	0.002870846825011597
1201	10	-0.005577590400584143
1201	11	0.10818491023896826
1201	12	0.03936466719041931
1201	13	-0.050182588213928625
1201	14	-0.006665510844030109
1201	15	0.09513369731428156
1201	16	0.0018826044557300747
1201	17	-0.02324655871549197
1201	18	0.08626559054570748
1201	19	0.007654471022952691
1201	20	-0.0019729023115786504
1201	21	0.042003347693743365
1201	22	0.09338405079422074
1201	23	-0.06724589763072686
1201	24	0.028560921519122928
1201	25	-0.0011585801426221565
1201	26	0.02779137890941996
1201	27	0.041254140047129795
1201	28	0.03270570158715458
1201	29	-0.08457020923937364
1201	30	0.031071433852581265
1201	31	0.02699949872018692
1201	32	0.1387812468345473
1201	33	0.0797234118402983
1201	34	0.08947666026288914
1201	35	0.08022077708447028
1201	36	0.06324022750983715
1201	37	0.0421088388013402
1201	38	0.16283560644130546
1201	39	1.4221286383154776
1201	40	0.1013461564959286
1201	41	0.04663534070940756
1201	42	0.11717770113902759
1201	43	0.10311660293209002
1201	44	0.0554476519079401
1201	45	0.09558545348036773
1201	46	0.12349932892495019
1201	47	0.10138073997933361
1201	48	0.1269748021553596
1201	49	0.06680108428204079
1201	50	0.052503858765021505
1201	51	0.09607212091028572
1201	52	0.08518955417287799
1201	53	0.1358475294044581
1201	54	0.16000716282852068
1201	55	0.15317406499305264
1201	56	0.16978100694268505
1201	57	0.1206954619120495
1201	58	0.06062074245453175
1201	59	0.08553694353454233
1201	60	0.20203646995626912
1201	61	0.23784942289728406
1202	1	0.022793537724183466
1202	2	0.014530237682514269
1202	3	-0.043678331102398715
1202	4	-0.002241444477100174
1202	5	0.033928709158075776
1202	6	-0.0010134520536766445
1202	7	0.14704973234285745
1202	8	0.0806236097631583
1202	9	-0.09649565445828756
1202	10	0.023949752186396722
1202	11	0.09933287945723634
1202	12	0.029448619353118488
1202	13	0.016541886568909092
1202	14	0.03157825250779922
1202	15	-0.030219508102298676
1202	16	-0.0773857968890681
1202	17	-0.051723792966583915
1202	18	0.026563280611618723
1202	19	0.05257441982147526
1202	20	-0.03620052285562281
1202	21	-0.09575730497292767
1202	22	-0.06566302804866098
1202	23	-0.11480676457861705
1202	24	-0.0026267744797294768
1202	25	-0.0115231841896275
1202	26	-0.03475352560938916
1202	27	0.10280370181379794
1202	28	0.002947126535481459
1202	29	0.007829762073896518
1202	30	0.04571296920137513
1202	31	0.035511635164724
1202	32	0.040783885144811904
1202	33	0.03851361401657982
1202	34	-0.058946764628942105
1202	35	-0.0042312302263027846
1202	36	-0.2597332828988854
1202	37	-0.12360920971836009
1202	38	-0.09615692414158161
1202	39	1.0291029919403272
1202	40	-0.1984345638453604
1202	41	-0.1842859163780588
1202	42	-0.1653918478924652
1202	43	-0.2128651679521809
1202	44	-0.14246038972260067
1202	45	-0.09773966501540343
1202	46	-0.10275033693979196
1202	47	-0.07898579824900054
1202	48	0.008061333950912797
1202	49	-0.02245464708843059
1202	50	0.026318086081317915
1202	51	-0.0720895157983037
1202	52	-0.03517215946831698
1202	53	0.02034430540670229
1202	54	-0.07184945565747114
1202	55	-0.1016441110956805
1202	56	-0.06441140715467242
1202	57	-0.001774292055735316
1202	58	-0.09657647667082614
1202	59	-0.061298279428754654
1202	60	-0.15046525233440633
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2402	60	-0.09108721104018258
2402	61	0.17095325515178672
2403	1	-0.12662252039788768
2403	2	0.05426885240015622
2403	3	0.12343826716124795
2403	4	0.060796693469619356
2403	5	0.038364654480389504
2403	6	0.049474315547520575
2403	7	-0.02047989343483181
2403	8	0.08024338587838997
2403	9	0.0027726009477859748
2403	10	-0.0036932697733931263
2403	11	-0.08875865757918433
2403	12	0.05475661933820675
2403	13	0.047897682932926955
2403	14	0.13066310580475005
2403	15	0.04337841632261075
2403	16	0.021813869110339112
2403	17	0.047985820360629816
2403	18	0.08548903630659055
2403	19	0.069504074675109
2403	20	-0.06890275909394461
2403	21	-0.05021393894779891
2403	22	0.006485572391952132
2403	23	-0.06003365013495454
2403	24	-0.031027262060945304
2403	25	0.011172454045990321
2403	26	-0.03721827102713604
2403	27	0.02355311563530904
2403	28	0.040884523791163474
2403	29	0.01965248827914934
2403	30	-0.032370105989762976
2403	31	0.07430341785559591
2403	32	0.15486297916821457
2403	33	-0.06185654511829597
2403	34	0.06255787820290948
2403	35	0.02032079521142549
2403	36	-0.029840975722720486
2403	37	0.024011595237965366
2403	38	0.05668711323964715
2403	39	1.2716739018309937
2403	40	0.08729846679994324
2403	41	0.031496356988000684
2403	42	-0.011593523114171944
2403	43	0.15851962980226375
2403	44	-0.012657919758424896
2403	45	-0.0701085716480705
2403	46	-0.022053408925117424
2403	47	-0.008210477015737183
2403	48	0.12138743557506615
2403	49	0.14888169075913282
2403	50	0.0336765365571523
2403	51	-0.07014813737489334
2403	52	0.013823043000897428
2403	53	0.026982567112568745
2403	54	0.0073713090398612735
2403	55	-0.04380833053705402
2403	56	0.04108655566540165
2403	57	-0.09385706157669205
2403	58	0.06047984492402492
2403	59	-0.017864783195584266
2403	60	0.03213316787262954
2403	61	-0.08279217562785135
2404	1	-0.0049479282492157495
2404	2	-0.0026062247123803672
2404	3	-0.02883030258878705
2404	4	0.15707684184656046
2404	5	0.23013226297367595
2404	6	0.09230178934645039
2404	7	0.05114532758928192
2404	8	0.013839089654695158
2404	9	0.07559174372754995
2404	10	0.022420216202341692
2404	11	0.12216273174909276
2404	12	3.633532158104913E-4
2404	13	0.14846519302774652
2404	14	0.014792189618013651
2404	15	0.1546081030174662
2404	16	0.06374964501242175
2404	17	0.012912573474835654
2404	18	0.07024491376230202
2404	19	0.07929930578735878
2404	20	0.046303509071670135
2404	21	0.0673386131287117
2404	22	0.1428707351562295
2404	23	0.031212897228670045
2404	24	0.1003951954789315
2404	25	0.11047514659623658
2404	26	0.07125045250869988
2404	27	0.09394377205926219
2404	28	0.1435611836063515
2404	29	0.06117266817721401
2404	30	0.08959448660583291
2404	31	0.09796763767531758
2404	32	0.11509808121651588
2404	33	0.05305880584450762
2404	34	0.06532464748256928
2404	35	0.16052577418906822
2404	36	0.13372110985109487
2404	37	0.23387504826779093
2404	38	0.11091352978984048
2404	39	1.4568191713514977
2404	40	0.1808081788736544
2404	41	0.10353717401613238
2404	42	0.15738750653257227
2404	43	0.14831002502718604
2404	44	0.20288330809093935
2404	45	0.1609716685572522
2404	46	0.2496702869609777
2404	47	0.11302590581694005
2404	48	0.17376628341917666
2404	49	0.15496008790313454
2404	50	0.08707176109932391
2404	51	0.13738797803245006
2404	52	0.11568837385237174
2404	53	0.16809910703136666
2404	54	0.08376946681889308
2404	55	0.015004004566257834
2404	56	0.12055829036235366
2404	57	0.06890225142171857
2404	58	0.175621023421364
2404	59	0.0211807669668147
2404	60	0.2706971920031691
2404	61	0.04367246605238506
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	20.0
18	92.0
19	115.0
20	190.0
21	253.0
22	361.0
23	580.0
24	876.0
25	1163.0
26	1566.0
27	1991.0
28	2704.0
29	3215.0
30	4145.0
31	5181.0
32	6954.0
33	8593.0
34	10796.0
35	13823.0
36	20831.0
37	30635.0
38	79692.0
39	519222.0
40	452758.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	33.10263956786952	14.230471306676595	20.28034954051395	32.38653958493994
2	30.71901313638622	18.85679374011596	24.77782096793431	25.64637215556351
3	27.070541227253	19.857980815915482	19.850603641579127	33.22087431525239
4	27.33477045387675	19.775991051308292	20.47107587601876	32.418162618796195
5	27.564530919340964	21.381949442380456	20.389634564262423	30.663885074016157
6	29.190595997972135	24.587328941916812	21.878535786556228	24.343539273554825
7	28.41436351767389	23.674533072444646	21.533181386547305	26.377922023334154
8	26.22471711563025	26.095788237534517	22.230409544339782	25.449085102495456
9	25.443251992907623	25.699222566585373	23.353540483067256	25.503984957439744
10	26.816177271631613	23.848840111892766	22.7267385454455	26.608244071030118
11	25.84453562014104	24.64728987723878	22.938446111074416	26.569728391545766
12	26.775173942470097	24.039960231917576	23.011531542969003	26.173334282643324
13	26.211077932917526	22.39510910917265	22.8025689700873	28.591243987822523
14	26.228405699634315	24.04836677220592	23.022168389864458	26.701059138295307
15	26.236211772759205	23.269646642230512	22.839626372174695	27.654515212835584
16	26.950596135221772	23.639877539011064	22.066224665647017	27.343301660120144
17	26.548454697754853	23.236792510287287	22.370747298541122	27.844005493416734
18	27.36414644879431	23.717252022073172	22.439543679268183	26.479057849864336
19	26.788470001089422	22.631006923386394	22.97850584897908	27.60201722654511
20	26.517916653442093	23.505458675420908	22.45790081826518	27.51872385287182
21	26.251566575938938	23.35422673125406	23.426025447798388	26.968181245008616
22	27.650912409854868	22.75736237078161	22.10293894364101	27.48878627572251
23	26.60215361837224	23.81178270980537	22.40445924071785	27.181604431104546
24	26.490295163923243	24.49425653158157	22.452839737887505	26.562608566607675
25	26.945277711774047	22.65905731802199	22.087584140461278	28.308080829742682
26	26.824154906803205	23.45879379871826	23.050218784500057	26.666832509978477
27	26.62102544350934	23.63095631258262	22.193009018158985	27.55500922574906
28	27.066657859814935	23.19321575042526	21.840020107071872	27.90010628268793
29	26.882829126774915	24.436783245936766	22.084753366690716	26.5956342605976
30	27.6191734312152	24.260160118858188	22.06442326415666	26.056243185769958
31	27.025568749630068	23.137372304224115	22.780523247086233	27.05653569905958
32	26.89157879115666	24.053513633606947	22.094103498235913	26.96080407700048
33	26.838137213609333	24.83557922349302	22.16144160156602	26.164841961331632
34	27.27613511883674	23.753108489833664	21.62307989901858	27.347676492311017
35	27.098568400501303	24.24780765149572	21.637662672988156	27.015961275014817
36	27.038693246202687	25.65701830309695	21.46035329772277	25.843935152977586
37	28.21792497420136	24.047508961972415	21.27060567407157	26.46396038975466
38	27.43311439156807	25.6946761723478	21.296597324146756	25.575612111937374
39	27.66107570899163	24.79350403076591	21.22752004759367	26.317900212648798
40	28.462143547680096	24.873923340930673	20.72040619030177	25.94352692108746
41	27.548404086951077	25.077052804224543	21.69179049872229	25.68275261010209
42	27.443837019486878	25.693646800067594	21.682697710247144	25.179818470198384
43	27.81518306957098	25.87867646743452	20.376853191783205	25.929287271211287
44	27.705211797635705	25.827122072400897	21.22497016964913	25.242695960314265
45	27.504655765042347	26.053755536092794	20.33902376048566	26.102564938379203
46	27.86759527483811	25.62459307627048	20.853881462635073	25.653930186256336
47	28.66055505468969	26.017212820145502	19.98474813404829	25.337483991116518
48	28.559161885089456	26.632176976544898	19.81370077348749	24.99496036487816
49	28.742990618129475	26.098876354375133	19.58989808356616	25.568234943929234
50	28.547324103867094	26.76222100794418	19.560646754603653	25.129808133585072
51	28.459741679026283	26.852376863485507	19.5787465505306	25.109134906957614
52	28.689291697512093	26.515257441718227	19.19968020834495	25.595770652424733
53	28.488135197755284	27.044354793743818	19.21735109915514	25.250158909345753
54	28.371901911115422	27.269444199015407	19.19813614992464	25.16051773994453
55	28.6240123387424	26.83582112597887	18.844117866557326	25.69604866872141
56	28.227532448816607	27.499079998524568	18.816925282155232	25.456462270503593
57	28.06334757012384	27.504484202995645	18.8493505089817	25.58281771789881
58	28.092084212946244	27.286171498568745	18.582571526361797	26.03917276212322
59	27.883207421087892	27.6383883804457	18.58900510311308	25.889399095353326
60	27.81278120091717	27.899248472454424	18.5460288104145	25.741941516213902
61	28.102721059841702	27.397172142784225	18.272215783879858	26.227891013494215
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.0
4	1.5
5	1.0
6	2.5
7	5.0
8	6.0
9	7.5
10	9.0
11	13.0
12	16.5
13	16.0
14	24.0
15	32.0
16	49.5
17	93.0
18	119.0
19	154.0
20	242.0
21	295.0
22	407.0
23	519.0
24	716.5
25	1139.5
26	1365.0
27	1745.0
28	2125.0
29	2582.5
30	3766.5
31	4493.0
32	5401.0
33	6309.0
34	7472.5
35	10328.0
36	12020.0
37	14345.5
38	16671.0
39	19056.5
40	24844.5
41	28247.0
42	31771.5
43	39351.0
44	43406.0
45	47660.5
46	51915.0
47	56336.5
48	64646.0
49	68534.0
50	71652.0
51	74770.0
52	76796.0
53	80156.0
54	81490.0
55	82099.0
56	82708.0
57	81624.0
58	78018.0
59	75496.0
60	72046.0
61	68203.0
62	67810.0
63	58821.0
64	49832.0
65	44683.0
66	35339.0
67	31144.0
68	27104.5
69	23065.0
70	19684.5
71	13662.0
72	11020.0
73	9238.5
74	7457.0
75	6098.0
76	3746.5
77	2754.0
78	2124.5
79	1495.0
80	1176.0
81	648.5
82	440.0
83	326.0
84	159.0
85	106.0
86	81.0
87	56.0
88	42.5
89	18.5
90	8.0
91	6.5
92	5.0
93	5.0
94	6.0
95	7.0
96	7.0
97	7.0
98	5.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.031738978639667376
3	8.578102335045236E-5
4	1.7156204670090472E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.105015702216324
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1165759.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.37232731240483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.27381731269193	78.00061186672353
2	8.973630892946796	15.680940311185102
3	1.3167074355243615	3.451313790939351
4	0.2655832647145779	0.9281851173335665
5	0.08964148708430221	0.3916092675150182
6	0.031781675745673074	0.16661033854725996
7	0.015189019912999726	0.09289700135752728
8	0.005591834110291118	0.03908572481288205
9	0.007334847504803764	0.05767764272786613
>10	0.019240106265511786	0.2928251652266984
>50	9.527936778003893E-4	0.05845845586808252
>100	4.23463856800173E-4	0.05161363666833868
>500	0.0	0.0
>1k	0.0	0.0
>5k	1.0586596420004324E-4	0.7881716810948278
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGACGTTATCTGGGGGGGGGGGGGGG	8521	0.7309400999692046	TruSeq Adapter, Index 7 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.837394349947116	0.0	0.0	0.0	0.0
2	0.8386810652973727	0.0	0.0	0.0	8.578102335045236E-5
3	0.8398819996242791	0.0	0.0	0.0	8.578102335045236E-5
4	0.8409113719044845	0.0	0.0	0.0	8.578102335045236E-5
5	0.8427127733948441	0.0	0.0	0.0	8.578102335045236E-5
6	0.8470018245623666	0.0	0.0	0.0	1.7156204670090472E-4
7	0.8511193136831884	0.0	0.0	0.0	1.7156204670090472E-4
8	0.8846596938132153	0.0	0.0	0.0	1.7156204670090472E-4
9	0.888777182934037	0.0	0.0	0.0	1.7156204670090472E-4
10	0.8916937377279524	0.0	0.0	0.0	1.7156204670090472E-4
11	0.895811226848774	0.0	0.0	0.0	1.7156204670090472E-4
12	0.9043893291838193	0.0	0.0	0.0	1.7156204670090472E-4
13	0.9169133585929854	0.0	0.0	0.0	1.7156204670090472E-4
14	0.9383586144305984	0.0	0.0	0.0	1.7156204670090472E-4
15	0.9640071404123837	0.0	0.0	0.0	1.7156204670090472E-4
16	0.9969470533789574	0.0	0.0	0.0	1.7156204670090472E-4
17	1.0328035211394464	0.0	0.0	0.0	1.7156204670090472E-4
18	1.0766376240715276	0.0	0.0	0.0	2.573430700513571E-4
19	1.1391719900940074	0.0	0.0	0.0	3.4312409340180944E-4
20	1.214573509619055	0.0	0.0	0.0	3.4312409340180944E-4
21	1.317339175592897	0.0	0.0	0.0	3.4312409340180944E-4
22	1.4480694551789863	0.0	0.0	0.0	3.4312409340180944E-4
23	1.5933825087346527	0.0	0.0	0.0	3.4312409340180944E-4
24	1.7707776650233882	0.0	8.578102335045236E-5	0.0	3.4312409340180944E-4
25	1.9960386323416761	0.0	8.578102335045236E-5	0.0	3.4312409340180944E-4
26	2.2532101403463325	0.0	8.578102335045236E-5	0.0	3.4312409340180944E-4
27	2.557732773240438	0.0	8.578102335045236E-5	0.0	3.4312409340180944E-4
28	2.9072904433935314	0.0	8.578102335045236E-5	0.0	3.4312409340180944E-4
29	3.306515326066537	0.0	8.578102335045236E-5	0.0	3.4312409340180944E-4
30	3.767502545551868	0.0	8.578102335045236E-5	0.0	3.4312409340180944E-4
31	4.2824460287246335	0.0	8.578102335045236E-5	0.0	3.4312409340180944E-4
32	4.86000965894323	0.0	8.578102335045236E-5	0.0	3.4312409340180944E-4
33	5.4936740784330205	0.0	8.578102335045236E-5	0.0	3.4312409340180944E-4
34	6.192103170552404	8.578102335045236E-5	8.578102335045236E-5	0.0	3.4312409340180944E-4
35	6.931106686716551	8.578102335045236E-5	8.578102335045236E-5	0.0	3.4312409340180944E-4
36	7.755204978044347	8.578102335045236E-5	1.7156204670090472E-4	0.0	3.4312409340180944E-4
37	8.626311270168191	8.578102335045236E-5	1.7156204670090472E-4	0.0	3.4312409340180944E-4
38	9.583455928712539	8.578102335045236E-5	1.7156204670090472E-4	0.0	3.4312409340180944E-4
39	10.567450047565577	8.578102335045236E-5	1.7156204670090472E-4	0.0	3.4312409340180944E-4
40	11.666648080778273	8.578102335045236E-5	1.7156204670090472E-4	0.0	3.4312409340180944E-4
41	12.797241968537236	8.578102335045236E-5	1.7156204670090472E-4	0.0	3.4312409340180944E-4
42	14.006582835731914	8.578102335045236E-5	1.7156204670090472E-4	0.0	5.146861401027142E-4
43	15.292183032685143	8.578102335045236E-5	1.7156204670090472E-4	0.0	5.146861401027142E-4
44	16.57503823689116	8.578102335045236E-5	1.7156204670090472E-4	8.578102335045236E-5	5.146861401027142E-4
45	17.884056653219062	8.578102335045236E-5	1.7156204670090472E-4	8.578102335045236E-5	5.146861401027142E-4
46	19.279799684154273	8.578102335045236E-5	1.7156204670090472E-4	8.578102335045236E-5	5.146861401027142E-4
47	20.672540379272217	8.578102335045236E-5	1.7156204670090472E-4	1.7156204670090472E-4	5.146861401027142E-4
48	22.095819118702924	8.578102335045236E-5	1.7156204670090472E-4	1.7156204670090472E-4	5.146861401027142E-4
49	23.580688632899253	8.578102335045236E-5	1.7156204670090472E-4	1.7156204670090472E-4	5.146861401027142E-4
50	25.07602343194434	8.578102335045236E-5	1.7156204670090472E-4	1.7156204670090472E-4	5.146861401027142E-4
>>END_MODULE