##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-37B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 754830 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.595935508657575 40.0 40.0 40.0 40.0 40.0 2 39.32885550388829 40.0 40.0 40.0 40.0 40.0 3 39.3231747545805 40.0 40.0 40.0 40.0 40.0 4 39.254006862472345 40.0 40.0 40.0 40.0 40.0 5 39.253866433501585 40.0 40.0 40.0 40.0 40.0 6 39.36942092921585 40.0 40.0 40.0 40.0 40.0 7 39.421437939668536 40.0 40.0 40.0 40.0 40.0 8 39.40315037823086 40.0 40.0 40.0 40.0 40.0 9 39.41811533722825 40.0 40.0 40.0 40.0 40.0 10-11 39.41939774518766 40.0 40.0 40.0 40.0 40.0 12-13 39.34971847965767 40.0 40.0 40.0 40.0 40.0 14-15 39.37685836545977 40.0 40.0 40.0 40.0 40.0 16-17 39.485409959858515 40.0 40.0 40.0 40.0 40.0 18-19 39.51020494680921 40.0 40.0 40.0 40.0 40.0 20-21 39.48646450194084 40.0 40.0 40.0 40.0 40.0 22-23 39.52826994157625 40.0 40.0 40.0 40.0 40.0 24-25 39.504365221308106 40.0 40.0 40.0 40.0 40.0 26-27 39.482468900282186 40.0 40.0 40.0 40.0 40.0 28-29 39.49850429898123 40.0 40.0 40.0 40.0 40.0 30-31 39.482641431976674 40.0 40.0 40.0 40.0 40.0 32-33 39.487858189161315 40.0 40.0 40.0 40.0 40.0 34-35 39.48632012415675 40.0 40.0 40.0 40.0 40.0 36-37 39.46841829340669 40.0 40.0 40.0 40.0 40.0 38-39 39.41245106340412 40.0 40.0 40.0 40.0 40.0 40-41 39.457675662886764 40.0 40.0 40.0 40.0 40.0 42-43 39.436868317281466 40.0 40.0 40.0 40.0 40.0 44-45 39.348679756283644 40.0 40.0 40.0 40.0 40.0 46-47 39.3324097074891 40.0 40.0 40.0 40.0 40.0 48-49 39.41496467522853 40.0 40.0 40.0 40.0 40.0 50-51 39.37771127999129 40.0 40.0 40.0 40.0 40.0 52-53 39.332989652467916 40.0 40.0 40.0 40.0 40.0 54-55 39.24615188316186 40.0 40.0 40.0 40.0 40.0 56-57 39.259603302018476 40.0 40.0 40.0 40.0 40.0 58-59 39.21094277897958 40.0 40.0 40.0 40.0 40.0 60-61 39.137811515388165 40.0 40.0 40.0 40.0 40.0 62-63 39.40954870629191 40.0 40.0 40.0 40.0 40.0 64-65 39.40840720144338 40.0 40.0 40.0 40.0 40.0 66-67 39.41047456725539 40.0 40.0 40.0 40.0 40.0 68-69 39.411838229084836 40.0 40.0 40.0 40.0 40.0 70-71 39.41073142436983 40.0 40.0 40.0 40.0 40.0 72-73 39.41511875796603 40.0 40.0 40.0 40.0 40.0 74-75 39.417453247281976 40.0 40.0 40.0 40.0 40.0 76-77 39.41587192766241 40.0 40.0 40.0 40.0 40.0 78-79 39.41301374260599 40.0 40.0 40.0 40.0 40.0 80-81 39.423276374759396 40.0 40.0 40.0 40.0 40.0 82-83 39.412416900603134 40.0 40.0 40.0 40.0 40.0 84-85 39.38984042047817 40.0 40.0 40.0 40.0 40.0 86-87 39.39204341001996 40.0 40.0 40.0 40.0 40.0 88-89 39.41337780066006 40.0 40.0 40.0 40.0 40.0 90-91 39.49915169593022 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.011287051342556254 1101 2 -0.07564258521239964 1101 3 0.019575884783705533 1101 4 0.059330971703005275 1101 5 0.06843998875248047 1101 6 0.13373096536945894 1101 7 0.13564809090134133 1101 8 0.006663165858540765 1101 9 -0.1014737600427651 1101 10-11 0.050794928024110675 1101 12-13 -9.579888188611108E-4 1101 14-15 -0.026659050423695874 1101 16-17 0.08738514102635975 1101 18-19 -0.05993661941256789 1101 20-21 0.022662871216077463 1101 22-23 0.058585266733182095 1101 24-25 3.527598621886341E-4 1101 26-27 0.006812245928735194 1101 28-29 0.02203659994329854 1101 30-31 0.059776313960838934 1101 32-33 0.05816261216422447 1101 34-35 -0.017229311363735178 1101 36-37 -0.02740097138110542 1101 38-39 -0.059413826731550046 1101 40-41 -0.043801384328581605 1101 42-43 -0.0443385488278949 1101 44-45 0.12028900109401519 1101 46-47 -0.08675014519209157 1101 48-49 -0.08309080313719619 1101 50-51 -0.06533138574977215 1101 52-53 0.020247283301145558 1101 54-55 0.004286348109957316 1101 56-57 0.05267916693453856 1101 58-59 0.10332990842202605 1101 60-61 0.03547943467649617 1101 62-63 -0.08704293009701303 1101 64-65 -0.032945798860140485 1101 66-67 -0.03686144969989158 1101 68-69 -0.03638948211397519 1101 70-71 0.018203656296634563 1101 72-73 -0.04502842384157901 1101 74-75 -0.18328149135648175 1101 76-77 -0.18139219452351085 1101 78-79 -0.11375624563420672 1101 80-81 -0.07618213214404079 1101 82-83 0.0010218463114526344 1101 84-85 0.20161660943465165 1101 86-87 -0.41087500399383003 1101 88-89 -0.003269033444865954 1101 90-91 -0.40326767245400674 1102 1 0.08196948904870283 1102 2 0.002882284551333214 1102 3 0.05773848690807881 1102 4 -0.1606538033479552 1102 5 -0.023400913015116487 1102 6 -0.10843021531788821 1102 7 -0.17527469176872046 1102 8 0.03989215555311887 1102 9 -0.1714397731189905 1102 10-11 -0.13294403119572706 1102 12-13 -0.10543858032221465 1102 14-15 -0.08895833249859919 1102 16-17 -5.579915524549506E-4 1102 18-19 -0.029087874062682317 1102 20-21 -0.0985726193163714 1102 22-23 -0.04731676179661548 1102 24-25 -0.1018995882838567 1102 26-27 -0.16865280668348248 1102 28-29 -0.05627662811865264 1102 30-31 -0.07938659547887994 1102 32-33 -0.011097041045005085 1102 34-35 -0.013988482752360198 1102 36-37 -0.1535442623369292 1102 38-39 -0.0886199357533144 1102 40-41 -0.07354216960199267 1102 42-43 -0.13098346624970247 1102 44-45 -0.09941983883933858 1102 46-47 -0.05672464100829444 1102 48-49 -0.019128486915796827 1102 50-51 -0.11553869855818277 1102 52-53 -0.055816784762846794 1102 54-55 -0.09961623581443746 1102 56-57 -0.1949252402653201 1102 58-59 0.009090861200689915 1102 60-61 -0.21190803401815117 1102 62-63 -0.04514916060023211 1102 64-65 -0.0378176971575499 1102 66-67 -0.12361036117482627 1102 68-69 -0.10806727560442653 1102 70-71 -0.12094825062576575 1102 72-73 -0.1366080840102839 1102 74-75 -0.15719520819261845 1102 76-77 -0.1776570182647248 1102 78-79 -0.07870254096236806 1102 80-81 -0.09217418619520856 1102 82-83 -0.018893676342891297 1102 84-85 -0.0847131772409142 1102 86-87 0.06984362874248262 1102 88-89 -0.33776270561832433 1102 90-91 -0.46140720733772866 1103 1 0.010984427204405733 1103 2 0.15485619630586456 1103 3 0.0900951580216045 1103 4 0.13190676453253758 1103 5 0.0706287656580713 1103 6 0.023252838851455238 1103 7 0.017485161320195175 1103 8 0.03717133601328726 1103 9 0.1367935058638281 1103 10-11 0.019649146991604027 1103 12-13 -0.03581228126264335 1103 14-15 -0.029171122985609088 1103 16-17 -0.03164154311132705 1103 18-19 -0.01796084209469484 1103 20-21 -0.05736504977242163 1103 22-23 0.025827497636491614 1103 24-25 0.00695460411695592 1103 26-27 0.0359949855328594 1103 28-29 -0.05165848163578346 1103 30-31 -0.048418332883557014 1103 32-33 -0.05061501605431573 1103 34-35 -0.03838292074254923 1103 36-37 0.08103353749830688 1103 38-39 -0.041663927516211174 1103 40-41 -0.08175866624659278 1103 42-43 0.05334973011965616 1103 44-45 -0.007021364592404211 1103 46-47 0.026019588597598897 1103 48-49 0.028533609919165315 1103 50-51 0.021566061364197253 1103 52-53 -0.05751689409994043 1103 54-55 -0.04566222179983015 1103 56-57 0.12494785808431175 1103 58-59 0.07918684089454331 1103 60-61 0.04141531072647098 1103 62-63 -0.07104743149589865 1103 64-65 -0.08642158564904179 1103 66-67 -0.10673679653366719 1103 68-69 -0.11568470566652422 1103 70-71 -0.0017550509085495491 1103 72-73 -0.10458084060406492 1103 74-75 -0.1701518374846529 1103 76-77 0.014522254542910673 1103 78-79 -0.045179381098435556 1103 80-81 -0.1933945091847704 1103 82-83 -0.08998156035175242 1103 84-85 -0.9656668903155889 1103 86-87 -0.42143658038866505 1103 88-89 -0.015240412769031764 1103 90-91 0.17465440546806832 1104 1 -0.15408386689614417 1104 2 -0.08829986297922687 1104 3 -0.11240411544540763 1104 4 -0.30813973357847857 1104 5 -0.06613380336256114 1104 6 -0.020978489250836674 1104 7 0.030155896571578467 1104 8 0.025721176176027427 1104 9 -0.002119600791210985 1104 10-11 -0.058235188957247885 1104 12-13 0.012865428705389093 1104 14-15 0.002273224587767686 1104 16-17 -0.03704694851673196 1104 18-19 -0.07297391968842248 1104 20-21 -0.0015673165553309332 1104 22-23 -0.023663929274299278 1104 24-25 -0.06654147260493204 1104 26-27 -0.028317714263707217 1104 28-29 -0.10751433749164363 1104 30-31 -0.12380339536539964 1104 32-33 -0.13759117182627278 1104 34-35 -0.015064195315055429 1104 36-37 -0.1932983131654069 1104 38-39 -0.11528864745623935 1104 40-41 -0.053279311963429166 1104 42-43 -0.031937190793328796 1104 44-45 -0.1085939427442213 1104 46-47 -0.05266889313944745 1104 48-49 -0.08581472217842645 1104 50-51 -0.04574033472427885 1104 52-53 -0.06239909269734767 1104 54-55 -0.15008336254351917 1104 56-57 -0.027133309198148936 1104 58-59 -0.124085335473751 1104 60-61 -0.016100151251485784 1104 62-63 -0.2778613025000638 1104 64-65 -0.2386615287886542 1104 66-67 -0.06577141733637148 1104 68-69 -0.08321132091012373 1104 70-71 -0.10896120731409553 1104 72-73 -0.1178643084946458 1104 74-75 -0.22088649501630897 1104 76-77 -0.14664497593793158 1104 78-79 -0.14286141951036768 1104 80-81 0.0384824059385096 1104 82-83 0.13030032314959072 1104 84-85 0.07335287743630658 1104 86-87 0.11852742974826214 1104 88-89 0.08291513058443911 1104 90-91 0.27855050936416603 1201 1 0.05893637915635708 1201 2 0.055961301410967224 1201 3 0.07126032318677034 1201 4 0.12962448225025724 1201 5 0.16679693382624095 1201 6 0.14541500101361748 1201 7 0.09421789405293168 1201 8 0.0678019666439198 1201 9 0.05924395631428325 1201 10-11 0.0386521499946042 1201 12-13 0.05705258360222132 1201 14-15 0.039945994131507234 1201 16-17 -0.07036826983805611 1201 18-19 0.05818330204944999 1201 20-21 0.027535851128476452 1201 22-23 0.0921217919307793 1201 24-25 0.0012488984112550838 1201 26-27 0.04878777832565362 1201 28-29 0.04566283968553364 1201 30-31 0.06742551096028393 1201 32-33 0.059455151512096904 1201 34-35 0.03848253136712998 1201 36-37 0.05314421253915924 1201 38-39 0.14606573817453494 1201 40-41 0.10116910549970015 1201 42-43 0.17246608495138105 1201 44-45 -0.010443120051419896 1201 46-47 0.09701136317735148 1201 48-49 0.10470871719603281 1201 50-51 0.013994551268972089 1201 52-53 0.060019700505272056 1201 54-55 0.006826797618487035 1201 56-57 0.13274938276913417 1201 58-59 0.035855095015215 1201 60-61 0.16421308568386905 1201 62-63 0.04879894607829982 1201 64-65 -0.06203923631780128 1201 66-67 -0.014949228060672226 1201 68-69 0.04178924614887336 1201 70-71 0.06547361964447163 1201 72-73 -0.0990088619651388 1201 74-75 -0.013254358074405559 1201 76-77 -0.044158311678728523 1201 78-79 -0.02896784661520968 1201 80-81 -0.22447309826939943 1201 82-83 -0.0377820507445179 1201 84-85 -0.17767857691460875 1201 86-87 0.08456358666872177 1201 88-89 0.16933612180750401 1201 90-91 -0.2305403997267348 1202 1 0.05094518003645021 1202 2 0.11121642692351941 1202 3 0.009615758730326718 1202 4 -7.40554253397363E-4 1202 5 0.01903495762861951 1202 6 0.03281974148766409 1202 7 0.08099097136066291 1202 8 0.021477334180673324 1202 9 -0.1364284109224414 1202 10-11 -0.04285440066045254 1202 12-13 -0.030683389995139976 1202 14-15 -0.04028682915082271 1202 16-17 -0.007733206331707265 1202 18-19 -0.02380169645040553 1202 20-21 -0.02355085754238928 1202 22-23 0.05052483223480664 1202 24-25 0.01281005452685946 1202 26-27 -0.04636973742591266 1202 28-29 -0.0034444710455403538 1202 30-31 0.08619458786797907 1202 32-33 -0.011054581263188368 1202 34-35 -0.034465924831458494 1202 36-37 -0.22719032782448778 1202 38-39 -0.1790786873986292 1202 40-41 -0.11312093896926712 1202 42-43 -0.2746048368937295 1202 44-45 -0.09493746337444975 1202 46-47 -0.10466558970257722 1202 48-49 -0.1267814115758057 1202 50-51 -0.03564050668922647 1202 52-53 -0.03656605195810414 1202 54-55 -0.015285424475791842 1202 56-57 -0.08658160525168057 1202 58-59 0.02133677124200517 1202 60-61 -0.07342919062441666 1202 62-63 -0.07336657020499615 1202 64-65 -0.15169032620393352 1202 66-67 -0.1255628050812163 1202 68-69 -0.2358837817467787 1202 70-71 -0.13695856573928467 1202 72-73 -0.08406640443501345 1202 74-75 0.00474901639499592 1202 76-77 0.11525985814336082 1202 78-79 0.10366291959911678 1202 80-81 0.054398486158390824 1202 82-83 0.032384274686300785 1202 84-85 -0.0038982620505763066 1202 86-87 -0.1727658703246675 1202 88-89 -0.14901546124995946 1202 90-91 -0.2678364370227726 1203 1 0.007380823600804831 1203 2 0.18472880018727977 1203 3 0.03431073924159023 1203 4 -0.03045383395140533 1203 5 -0.05714041321243002 1203 6 -0.01752185654878957 1203 7 0.05614055680378982 1203 8 0.017875388082977395 1203 9 -0.0805974533773508 1203 10-11 0.04428700070435099 1203 12-13 0.018333053499432594 1203 14-15 -0.0509673685704044 1203 16-17 0.06444132389664503 1203 18-19 -0.037546507054813105 1203 20-21 -0.05014990511234174 1203 22-23 -0.07111658300802759 1203 24-25 0.048042828905614954 1203 26-27 -0.01482455647610692 1203 28-29 0.001331953733497926 1203 30-31 -0.024740910968255037 1203 32-33 6.877968579033222E-5 1203 34-35 -0.04431968756437499 1203 36-37 0.025545332195562764 1203 38-39 0.021880299166582518 1203 40-41 0.053253473775669136 1203 42-43 -0.07675019898471191 1203 44-45 0.060904036744616974 1203 46-47 -0.00869191065901731 1203 48-49 0.018415437758214637 1203 50-51 0.09910952335612677 1203 52-53 0.06139340716715935 1203 54-55 -0.08754445328349902 1203 56-57 -0.05106968031446968 1203 58-59 -0.05746826261537308 1203 60-61 0.00925208744923367 1203 62-63 -0.1858593930189798 1203 64-65 -0.0704244234508522 1203 66-67 -0.06995165472486065 1203 68-69 -0.060892207185489156 1203 70-71 0.007533607842169943 1203 72-73 -0.05880316215457526 1203 74-75 0.052044301840645346 1203 76-77 -0.08405886119017936 1203 78-79 0.11836131613894452 1203 80-81 -0.022918083740222528 1203 82-83 0.005480381342188423 1203 84-85 0.0648663771663962 1203 86-87 0.17273264718877357 1203 88-89 0.2585284109997943 1203 90-91 0.32477547084337033 1204 1 0.03992675535933188 1204 2 -0.07258463034756346 1204 3 -0.14316487366362196 1204 4 -0.2383895159999625 1204 5 -0.08123806179931137 1204 6 -0.01633478026371904 1204 7 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-0.10304501910199093 2401 28-29 -0.1297833911111823 2401 30-31 -0.0722415129768379 2401 32-33 -0.0924917402007992 2401 34-35 -0.08086665265788895 2401 36-37 -0.05032670325322641 2401 38-39 0.09620014309469127 2401 40-41 -0.025238176794907474 2401 42-43 0.056690068617186284 2401 44-45 0.07010201814951955 2401 46-47 0.05453539255220363 2401 48-49 0.01959351422096489 2401 50-51 0.1235187341441133 2401 52-53 0.016364340456327398 2401 54-55 0.0889801277847937 2401 56-57 0.05518497945027434 2401 58-59 0.016039785261924067 2401 60-61 0.10442680321038011 2401 62-63 0.044862935083052946 2401 64-65 0.08930440945555063 2401 66-67 0.03379452115814274 2401 68-69 -0.04479259360130072 2401 70-71 0.271125715459668 2401 72-73 0.1855384889539451 2401 74-75 0.19280834372278122 2401 76-77 0.06334048855481456 2401 78-79 0.020863143464410427 2401 80-81 -0.019874166424855844 2401 82-83 -0.04329612643869751 2401 84-85 0.1115854259400777 2401 86-87 -0.12087994936499769 2401 88-89 -0.27174760377479856 2401 90-91 0.1937020245156873 2402 1 0.0682088490785091 2402 2 0.12489406857303464 2402 3 0.05964815050453609 2402 4 0.16519419208748332 2402 5 0.16710549716665213 2402 6 0.08838740016436475 2402 7 0.007153492071331868 2402 8 0.054956956028206605 2402 9 0.08087452956269203 2402 10-11 0.05750706860748522 2402 12-13 0.08356426088460012 2402 14-15 0.08943219370687672 2402 16-17 0.12048877191707419 2402 18-19 0.03982449100911367 2402 20-21 0.14714519295437611 2402 22-23 0.08717770134338565 2402 24-25 0.11904790761153805 2402 26-27 0.06541198214731736 2402 28-29 0.12557225228284352 2402 30-31 0.11322452016056417 2402 32-33 0.1253889216605586 2402 34-35 0.11597240301698974 2402 36-37 0.06112493554774545 2402 38-39 -0.21218457482102338 2402 40-41 0.08389024467468431 2402 42-43 0.1491950027956861 2402 44-45 0.12352807304456803 2402 46-47 0.08820902478897352 2402 48-49 0.12572709684880579 2402 50-51 0.13696820976417 2402 52-53 0.1636485445822231 2402 54-55 0.113323712028091 2402 56-57 0.079602954685285 2402 58-59 0.09005167947395876 2402 60-61 0.17377314248404474 2402 62-63 0.20870200814363926 2402 64-65 0.13326102589091704 2402 66-67 0.11871547356139445 2402 68-69 -0.06168354540371013 2402 70-71 0.11409455601970109 2402 72-73 0.07331862932006317 2402 74-75 0.10825696221147041 2402 76-77 0.2236919517449678 2402 78-79 -4.332416239947179E-4 2402 80-81 0.044244945794929436 2402 82-83 0.12012394128861814 2402 84-85 0.17398719591537315 2402 86-87 0.30724787127517317 2402 88-89 0.350741217540957 2402 90-91 0.11254260422582973 2403 1 -8.146058712199533E-4 2403 2 0.0044057458554149775 2403 3 0.013967967000795056 2403 4 0.09879705908240766 2403 5 -0.047570284455872525 2403 6 0.09573809006010947 2403 7 0.08071434795363785 2403 8 0.11413766404344727 2403 9 -0.01480490105372212 2403 10-11 0.03132039270540332 2403 12-13 0.07294541260143461 2403 14-15 0.04759926442307716 2403 16-17 0.012002100532313875 2403 18-19 0.027128928526991558 2403 20-21 0.0767903822553464 2403 22-23 -0.01612722304163583 2403 24-25 0.08525205049951268 2403 26-27 -7.630490975074622E-4 2403 28-29 0.008197118645341561 2403 30-31 -0.00252563167170905 2403 32-33 0.023793874151792238 2403 34-35 -0.009536283766522047 2403 36-37 0.0016086794663081605 2403 38-39 0.01657105120834501 2403 40-41 0.11284898344698746 2403 42-43 0.001234107833681719 2403 44-45 0.014058804252059076 2403 46-47 -0.026779503529517967 2403 48-49 0.09999409800831671 2403 50-51 -0.05392525490132982 2403 52-53 0.02907383523722018 2403 54-55 -0.005971237208086677 2403 56-57 -0.009216440716073748 2403 58-59 0.06385934596935527 2403 60-61 0.06200941199438148 2403 62-63 0.19656041907502697 2403 64-65 0.02559883554812359 2403 66-67 0.1230582269424616 2403 68-69 -0.027134814981188526 2403 70-71 0.05015079618758023 2403 72-73 0.06740338143011826 2403 74-75 -0.00434850404498377 2403 76-77 0.2579187195359367 2403 78-79 0.17167215093861898 2403 80-81 0.12772041901500586 2403 82-83 -0.1571851015314749 2403 84-85 -0.16399910412626184 2403 86-87 0.042920663307022267 2403 88-89 0.23148592169314952 2403 90-91 -0.06475951394585167 2404 1 -0.01648102883248015 2404 2 -0.09423370992689684 2404 3 -0.09017836510906108 2404 4 0.09511549916984308 2404 5 0.060740070055089745 2404 6 0.12931871634590664 2404 7 -0.07120195593834922 2404 8 -0.10844083817031702 2404 9 -0.012442071943176813 2404 10-11 0.06957348263022567 2404 12-13 -0.015793046957689683 2404 14-15 0.0027549946547935633 2404 16-17 -0.037768234952309854 2404 18-19 6.992622796957448E-4 2404 20-21 0.01255730586421322 2404 22-23 -0.024444752635766065 2404 24-25 0.050036618627537166 2404 26-27 0.03820693060195168 2404 28-29 0.01960178962448822 2404 30-31 0.03950365503843045 2404 32-33 -0.02993982773224957 2404 34-35 0.06212767738728786 2404 36-37 0.09781731695161966 2404 38-39 0.10562405978879497 2404 40-41 0.0483850404573829 2404 42-43 0.021090440518221953 2404 44-45 0.07039372835451019 2404 46-47 0.06356235375112362 2404 48-49 0.05932475062984821 2404 50-51 0.09321115844210226 2404 52-53 0.03995296298806039 2404 54-55 0.04466625324347717 2404 56-57 0.023309388692510424 2404 58-59 -0.1445998805968287 2404 60-61 -0.10705046939025209 2404 62-63 0.06682453846499214 2404 64-65 0.028217742249452726 2404 66-67 0.04971714082072509 2404 68-69 0.017222718713455265 2404 70-71 0.01511215431749946 2404 72-73 -0.10191309696353557 2404 74-75 0.03632518920804273 2404 76-77 0.07303056417534037 2404 78-79 -0.17942986231423674 2404 80-81 -0.09724625994027747 2404 82-83 -0.34428337075791404 2404 84-85 -0.5088814248706512 2404 86-87 -0.08755396122666781 2404 88-89 -0.21714941507545404 2404 90-91 -0.5609441817929195 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 12.0 31 401.0 32 2969.0 33 5448.0 34 7093.0 35 9158.0 36 13043.0 37 19784.0 38 48830.0 39 321703.0 40 326389.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 33.23927241895526 13.359034484585933 20.74983771180266 32.651855384656145 2 30.136928218025126 18.971859091402965 25.2331383777968 25.65807431277511 3 27.143065325967434 19.312427963912405 20.18361750327888 33.36088920684128 4 27.35838183217656 19.961872159124784 20.023210554973378 32.65653545372528 5 27.707033371752583 21.583800325901194 20.639084297126505 30.070082005219717 6 28.46680709563743 24.742127366426878 22.263556032484136 24.527509505451558 7 27.6581481923082 23.85000596160725 21.884795251911026 26.607050594173522 8 26.25147384179219 25.466528887299127 22.633970562908203 25.648026708000476 9 25.450366307645428 25.157320191301352 23.672217585416583 25.72009591563663 10-11 25.899606533921542 24.053892929533802 23.224169680590332 26.82233085595432 12-13 26.080508193897963 23.4099731065273 23.282792151875256 27.22672654769948 14-15 26.20272114250891 23.363406329902098 23.34114966283799 27.092722864751 16-17 26.766622948213502 23.108117059470345 22.586542665235882 27.53871732708027 18-19 26.665540585297354 23.29107216194375 22.6774902958282 27.3658969569307 20-21 26.422505729766964 23.47316614337003 22.88747135116516 27.21685677569784 22-23 26.63573254905078 22.87137501159201 22.71763178464025 27.775260654716956 24-25 26.630830783090232 23.084270630473085 22.775856815441887 27.50904177099479 26-27 26.621755892055166 23.411827828782638 22.774929454314215 27.191486824847978 28-29 26.91970377435979 23.067313169852817 22.529841156286846 27.483141899500552 30-31 26.800124002580738 23.151189086713046 22.611526145864662 27.43716076484156 32-33 26.678342403038197 23.34244326852674 22.86236130593022 27.11685302250485 34-35 26.908579004195882 23.05397241926005 22.556343706862382 27.481104869681687 36-37 26.779701788110543 23.210962919659845 22.572736611198017 27.436598681031594 38-39 27.005995341748857 23.287733271990106 22.72622710412054 26.980044282140497 40-41 27.009396777019624 23.0885882302366 22.45991286597993 27.44210212676384 42-43 26.91227878816742 23.20681976521339 22.6450094979272 27.235891948691993 44-45 26.857346071932326 23.48888968804817 22.674066881640513 26.979697358378985 46-47 27.147528990868896 23.023418175015824 22.51483574207104 27.314217092044235 48-49 27.025444960387112 23.295147344521276 22.528915524343894 27.150492170747714 50-51 26.97107279605005 23.49463532693791 22.696238955424487 26.838052921587558 52-53 27.258430021311618 23.140368156002218 22.492412809661527 27.108789013024637 54-55 27.268461438418516 23.2313174665106 22.579373130446093 26.920847964624787 56-57 27.177635307058967 23.530705896732186 22.513472371915245 26.778186424293605 58-59 27.368114808437983 23.157470513328025 22.38876620991935 27.085648468314645 60-61 27.50164027222086 23.29546770271835 22.371254711762358 26.83163731329843 62-63 27.471829823889205 23.53415799909945 22.402176983378965 26.59183519363238 64-65 27.658240046992454 23.103398692410533 22.27950107232126 26.95886018827575 66-67 27.562763710412884 23.294173970064914 22.287628304650745 26.855434014871456 68-69 27.480609138574536 23.385902451064723 22.480122964614015 26.653365445746722 70-71 27.785304038845275 23.1145320119685 22.16685127526503 26.93331267392119 72-73 27.84191934757355 23.226571205044923 22.09004053957943 26.841468907802092 74-75 27.78121271287558 23.331163900639986 22.09675669812344 26.790866688360992 76-77 27.922769556558347 23.040877807749517 22.01924773741397 27.017104898278166 78-79 27.9493663321219 23.06491313913053 21.8711598008419 27.11456072790567 80-81 28.065361840618365 23.162319757718212 21.738914252135785 27.03340414952764 82-83 28.80975841799463 22.413441609187593 21.18231343368104 27.594486539136735 84-85 28.822569882108695 22.30814858752873 20.787424927708166 28.08185660265441 86-87 29.09328455709433 21.46851319571466 20.706558662137446 28.73164358505357 88-89 28.627544339959528 20.77303722345979 20.742428622442908 29.856989814137773 90-91 23.45799722969119 24.590564654118797 23.42540536136234 28.526032754827668 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 1.5 8 4.5 9 5.0 10 5.5 11 9.0 12 10.0 13 13.0 14 20.5 15 31.5 16 45.0 17 64.5 18 93.5 19 129.0 20 168.5 21 239.0 22 343.5 23 493.5 24 699.5 25 914.5 26 1165.5 27 1502.5 28 1969.0 29 2480.5 30 3048.0 31 3785.5 32 4621.0 33 5494.0 34 6647.5 35 8032.0 36 9657.0 37 11496.5 38 13443.5 39 15724.5 40 18279.5 41 21163.0 42 24038.5 43 26807.0 44 29911.0 45 32931.5 46 35787.5 47 38609.5 48 40979.0 49 43042.0 50 44845.0 51 46287.0 52 47386.0 53 47979.0 54 48562.0 55 49130.0 56 49285.5 57 48934.5 58 48226.5 59 47633.0 60 46692.5 61 45202.5 62 43435.0 63 41356.0 64 38685.0 65 35487.5 66 31960.0 67 28604.5 68 25421.0 69 22016.5 70 18682.5 71 15664.0 72 12938.0 73 10382.5 74 8132.0 75 6338.5 76 4925.0 77 3693.0 78 2768.5 79 2067.5 80 1435.5 81 965.0 82 672.5 83 466.5 84 320.5 85 230.5 86 163.0 87 112.5 88 72.5 89 40.5 90 28.5 91 24.5 92 15.0 93 8.5 94 5.5 95 4.0 96 3.5 97 2.5 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016957460620272112 3 0.0 4 1.3248016109587588E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.053526844251252856 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 4.1877011405204055E-4 72-73 0.0 74-75 8.135306416316171E-4 76-77 0.0 78-79 3.7351341660192436E-4 80-81 4.5201621834191406E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 5904.0 32-33 13457.0 34-35 15973.0 36-37 18541.0 38-39 20809.0 40-41 22908.0 42-43 25143.0 44-45 26536.0 46-47 27680.0 48-49 29215.0 50-51 29888.0 52-53 30491.0 54-55 30708.0 56-57 30591.0 58-59 30499.0 60-61 31230.0 62-63 30917.0 64-65 30212.0 66-67 29469.0 68-69 28762.0 70-71 28046.0 72-73 26970.0 74-75 25786.0 76-77 25160.0 78-79 23654.0 80-81 22543.0 82-83 21243.0 84-85 19922.0 86-87 18806.0 88-89 17267.0 90-91 16500.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.88102532507992 #Duplication Level Percentage of deduplicated Percentage of total 1 99.88457031156057 99.76573296873707 2 0.11257043489083937 0.22487300916374375 3 0.002292581555809743 0.006869561893069323 4 3.0589271189186923E-4 0.0012221151081291666 5 2.607792809010175E-4 0.0013023450979965329 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0018547222553422626 0.0 0.0 0.0 0.0 2 0.0019872024164381383 0.0 0.0 0.0 1.324801610958759E-4 3 0.0019872024164381383 0.0 0.0 0.0 1.324801610958759E-4 4 0.0021196825775340144 0.0 0.0 0.0 1.324801610958759E-4 5 0.00225216273862989 0.0 0.0 0.0 1.324801610958759E-4 6 0.002384642899725766 0.0 0.0 0.0 2.649603221917518E-4 7 0.0026496032219175177 0.0 0.0 0.0 2.649603221917518E-4 8 0.007948809665752553 0.0 0.0 0.0 2.649603221917518E-4 9 0.008213769987944305 0.0 0.0 0.0 2.649603221917518E-4 10-11 0.00834625014904018 0.0 0.0 0.0 2.649603221917518E-4 12-13 0.00834625014904018 0.0 0.0 0.0 2.649603221917518E-4 14-15 0.00834625014904018 0.0 0.0 0.0 2.649603221917518E-4 16-17 0.008478730310136058 0.0 0.0 0.0 2.649603221917518E-4 18-19 0.008478730310136058 0.0 0.0 0.0 4.6368056383556564E-4 20-21 0.008544970390683995 0.0 0.0 0.0 5.299206443835036E-4 22-23 0.008677450551779872 0.0 0.0 0.0 5.299206443835036E-4 24-25 0.008942410873971622 0.0 1.324801610958759E-4 0.0 5.299206443835036E-4 26-27 0.00933985135725925 0.0 1.324801610958759E-4 0.0 5.299206443835036E-4 28-29 0.009406091437807188 0.0 1.324801610958759E-4 0.0 5.299206443835036E-4 30-31 0.009406091437807188 0.0 1.324801610958759E-4 0.0 5.299206443835036E-4 32-33 0.009472331518355127 0.0 1.324801610958759E-4 0.0 5.299206443835036E-4 34-35 0.009604811679451002 1.324801610958759E-4 1.324801610958759E-4 0.0 5.299206443835036E-4 36-37 0.00967105175999894 1.324801610958759E-4 1.324801610958759E-4 0.0 5.299206443835036E-4 38-39 0.009803531921094816 1.324801610958759E-4 1.324801610958759E-4 0.0 5.299206443835036E-4 40-41 0.009936012082190693 1.324801610958759E-4 1.324801610958759E-4 0.0 5.299206443835036E-4 42-43 0.010200972404382443 1.324801610958759E-4 1.324801610958759E-4 0.0 6.624008054793794E-4 44-45 0.010532172807122132 1.324801610958759E-4 1.324801610958759E-4 1.324801610958759E-4 6.624008054793794E-4 46-47 0.010730893048765948 1.324801610958759E-4 1.324801610958759E-4 1.9872024164381384E-4 6.624008054793794E-4 48-49 0.010730893048765948 1.324801610958759E-4 1.324801610958759E-4 2.649603221917518E-4 6.624008054793794E-4 50-51 0.010863373209861823 1.324801610958759E-4 1.324801610958759E-4 2.649603221917518E-4 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