##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-37A_S50_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1800986
Sequences flagged as poor quality	0
Sequence length	61
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.481790530298404	40.0	40.0	40.0	40.0	40.0
2	39.19986274185363	40.0	40.0	40.0	40.0	40.0
3	39.15535156852968	40.0	40.0	40.0	40.0	40.0
4	39.0837407953199	40.0	40.0	40.0	40.0	40.0
5	39.109396741562676	40.0	40.0	40.0	40.0	40.0
6	39.22327436193285	40.0	40.0	40.0	40.0	40.0
7	39.25575212689049	40.0	40.0	40.0	40.0	40.0
8	39.24184418979381	40.0	40.0	40.0	40.0	40.0
9	39.25990540737129	40.0	40.0	40.0	40.0	40.0
10	39.26710812854736	40.0	40.0	40.0	40.0	40.0
11	39.27452850827269	40.0	40.0	40.0	40.0	40.0
12	39.26869836856034	40.0	40.0	40.0	40.0	40.0
13	39.10086363802939	40.0	40.0	40.0	40.0	40.0
14	39.2643918386928	40.0	40.0	40.0	40.0	40.0
15	39.1718714082175	40.0	40.0	40.0	40.0	40.0
16	39.34798382663719	40.0	40.0	40.0	40.0	40.0
17	39.30535162405482	40.0	40.0	40.0	40.0	40.0
18	39.38245605462785	40.0	40.0	40.0	40.0	40.0
19	39.33165055142017	40.0	40.0	40.0	40.0	40.0
20	39.33760062543518	40.0	40.0	40.0	40.0	40.0
21	39.32078983401315	40.0	40.0	40.0	40.0	40.0
22	39.37817173481637	40.0	40.0	40.0	40.0	40.0
23	39.3759218561388	40.0	40.0	40.0	40.0	40.0
24	39.372816890303426	40.0	40.0	40.0	40.0	40.0
25	39.33294761869332	40.0	40.0	40.0	40.0	40.0
26	39.29422660698084	40.0	40.0	40.0	40.0	40.0
27	39.3135471347362	40.0	40.0	40.0	40.0	40.0
28	39.322282349779506	40.0	40.0	40.0	40.0	40.0
29	39.328334590052336	40.0	40.0	40.0	40.0	40.0
30	39.3279414720603	40.0	40.0	40.0	40.0	40.0
31	39.277535749861464	40.0	40.0	40.0	40.0	40.0
32	39.299361571938924	40.0	40.0	40.0	40.0	40.0
33	39.297047284098824	40.0	40.0	40.0	40.0	40.0
34	39.29285180451153	40.0	40.0	40.0	40.0	40.0
35	39.2956213985006	40.0	40.0	40.0	40.0	40.0
36	39.263283556896056	40.0	40.0	40.0	40.0	40.0
37	39.182547782159325	40.0	40.0	40.0	40.0	40.0
38	39.24734339967107	40.0	40.0	40.0	40.0	40.0
39	38.034068004970614	40.0	40.0	40.0	40.0	40.0
40	39.24812519364393	40.0	40.0	40.0	40.0	40.0
41	39.265495678478345	40.0	40.0	40.0	40.0	40.0
42	39.21780180412285	40.0	40.0	40.0	40.0	40.0
43	39.242308379965195	40.0	40.0	40.0	40.0	40.0
44	39.19597376103979	40.0	40.0	40.0	40.0	40.0
45	39.05367615295177	40.0	40.0	40.0	40.0	40.0
46	39.052336886572135	40.0	40.0	40.0	40.0	40.0
47	39.1280420836142	40.0	40.0	40.0	40.0	40.0
48	39.20644358146038	40.0	40.0	40.0	40.0	40.0
49	39.18642343693954	40.0	40.0	40.0	40.0	40.0
50	39.17538281807854	40.0	40.0	40.0	40.0	40.0
51	39.13072283737908	40.0	40.0	40.0	40.0	40.0
52	39.13217315403895	40.0	40.0	40.0	40.0	40.0
53	39.06895667151216	40.0	40.0	40.0	40.0	40.0
54	38.9709836722773	40.0	40.0	40.0	40.0	40.0
55	39.00586012328802	40.0	40.0	40.0	40.0	40.0
56	39.00257969800987	40.0	40.0	40.0	40.0	40.0
57	38.89581373758597	40.0	40.0	40.0	40.0	40.0
58	38.83768557889956	40.0	40.0	40.0	40.0	40.0
59	38.75805364394837	40.0	40.0	40.0	40.0	40.0
60	38.71842424094357	40.0	40.0	40.0	40.0	40.0
61	38.59933169941355	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.014878852425482592
1101	2	0.050263621823354754
1101	3	0.06535683572820972
1101	4	-0.0274855172323214
1101	5	-0.0020979214426048998
1101	6	0.00799018450477007
1101	7	-0.06403010594750924
1101	8	-0.0306140237460113
1101	9	-0.05932263909418367
1101	10	-0.1208020243886665
1101	11	-0.046981690228790285
1101	12	-0.00190993475545298
1101	13	0.02669903015695496
1101	14	-0.021141222842118168
1101	15	0.07567351211758933
1101	16	0.019303668515533445
1101	17	0.009374651991315375
1101	18	0.046921732732535304
1101	19	-0.0066848235363110575
1101	20	-0.04017960360889106
1101	21	0.01973701516324411
1101	22	-0.026571570227083896
1101	23	0.05729701461022074
1101	24	-0.011909672555091788
1101	25	0.0215071247977221
1101	26	0.006943345094249764
1101	27	-0.019072393508018592
1101	28	-0.06509325626597473
1101	29	0.0649423975040122
1101	30	0.012057300113717417
1101	31	0.11208104330196988
1101	32	0.08195408724318298
1101	33	0.035153322796723785
1101	34	0.018317457418120853
1101	35	0.050474499818491836
1101	36	-0.04168286530887855
1101	37	-0.10362509329245029
1101	38	0.04013744561029142
1101	39	1.1606172628722646
1101	40	-0.061141625052627546
1101	41	0.030471533467462564
1101	42	-0.03829501505995836
1101	43	0.008524238500100978
1101	44	-0.04724250192955992
1101	45	-0.007938406853035929
1101	46	0.10472868557042148
1101	47	-0.006617999639651373
1101	48	0.05887931670847735
1101	49	-0.004305420160456208
1101	50	0.011264573568830372
1101	51	-0.008300472239419321
1101	52	-0.007571380586718135
1101	53	-0.03311252149718058
1101	54	-0.005090887911308073
1101	55	-0.04963314356848514
1101	56	0.0328470007955346
1101	57	0.09536493909320853
1101	58	-0.06188242618282658
1101	59	-0.006126285687784616
1101	60	-0.04279641618206398
1101	61	0.14347743735490326
1102	1	-0.00442874818101302
1102	2	-0.005369261842851358
1102	3	0.012002577479400145
1102	4	-0.05867428429677091
1102	5	-0.11249490567004727
1102	6	-0.0860837977100033
1102	7	0.0793107915242004
1102	8	-0.02667397239348901
1102	9	-0.028569095067290107
1102	10	-0.03500630743948108
1102	11	-0.05475845121912215
1102	12	-0.1262426086023538
1102	13	-0.1658515288860869
1102	14	-0.09305303981277291
1102	15	-0.09979287973138895
1102	16	0.016169682537693575
1102	17	0.05580501535504112
1102	18	0.013675396493276537
1102	19	-0.014734074059433055
1102	20	-0.06770175912225795
1102	21	-0.08148417506723149
1102	22	-0.008294103187154178
1102	23	-0.04017322772728704
1102	24	0.027070347096461944
1102	25	0.014239945366092854
1102	26	-0.012987351813769976
1102	27	-0.05143141640378701
1102	28	-0.025778029351322118
1102	29	-0.020243246977685203
1102	30	-0.03952637008985249
1102	31	-0.021449755854952457
1102	32	-0.03158567294702408
1102	33	0.03671770254688056
1102	34	0.045376832103343645
1102	35	-0.050058695915943474
1102	36	0.01821209199555085
1102	37	-0.06295668383309305
1102	38	0.046394964610911416
1102	39	1.2513988018133233
1102	40	0.035878902650551936
1102	41	0.0036138533012746166
1102	42	0.0829072363165011
1102	43	0.01603492427096853
1102	44	0.03862899894373584
1102	45	0.02023373241073756
1102	46	0.09046206489675512
1102	47	-0.009970784972061608
1102	48	0.01668192500584098
1102	49	0.08097216281225172
1102	50	-0.013902816252276295
1102	51	0.05466860411846852
1102	52	0.0415567954486491
1102	53	-0.027811447883671292
1102	54	0.08352546393135896
1102	55	-0.09915401811971947
1102	56	0.006398411279278093
1102	57	-0.06797293652422809
1102	58	0.006013793078359697
1102	59	-0.04122469550824093
1102	60	-0.05472513843184856
1102	61	-0.06087213226842891
1103	1	-0.014237813043415315
1103	2	0.021167783373449822
1103	3	-0.051555273485305975
1103	4	-0.005102218553908244
1103	5	-0.017288921576323446
1103	6	-0.050170869494635895
1103	7	9.016447311438469E-5
1103	8	-0.051276315420970775
1103	9	-0.007841460439159675
1103	10	-0.049879387021988464
1103	11	-0.06755211320249543
1103	12	0.029100855733886988
1103	13	-0.0107221541530933
1103	14	-0.07766088937914617
1103	15	-0.029577396616979
1103	16	-0.0237541709980178
1103	17	0.003397061829844006
1103	18	-0.04324025220304151
1103	19	-0.010261578305062358
1103	20	-0.08757568734823451
1103	21	0.07783682336181386
1103	22	-0.04378572331504671
1103	23	-0.07684149685141506
1103	24	-0.02778866718485773
1103	25	0.0022289882216242063
1103	26	-0.02930191983977437
1103	27	-0.030870376275487388
1103	28	-0.027202520816189235
1103	29	-0.00134393273947353
1103	30	-0.03211725450171343
1103	31	0.06184315440389554
1103	32	-0.03196310230951127
1103	33	-0.09361597345839812
1103	34	0.031058625518447514
1103	35	-0.012224868022769897
1103	36	0.005441695380099532
1103	37	-0.0036086590025590226
1103	38	-0.06963340322588607
1103	39	1.1553297087335253
1103	40	-0.0963600020593205
1103	41	-0.0638061692842129
1103	42	0.018690119563416374
1103	43	-0.006791294761981703
1103	44	0.03794019939814319
1103	45	-0.07784028087246497
1103	46	-0.02164385834537086
1103	47	-0.07836337226420653
1103	48	-0.03736641777668126
1103	49	0.046344857129909656
1103	50	-0.02026884775430915
1103	51	-0.034570837744148264
1103	52	0.060303257425083245
1103	53	0.023741955417747818
1103	54	0.013789150959361507
1103	55	-0.0035844674475100646
1103	56	-0.03101603726158686
1103	57	0.028127257410091033
1103	58	0.030396621934940526
1103	59	-0.03779648929944557
1103	60	-0.1287446900202056
1103	61	0.09833724061297744
1104	1	0.03371116079313197
1104	2	-0.013660320307693041
1104	3	-0.01368726517154073
1104	4	-0.0037226136883319327
1104	5	-0.049295391880839645
1104	6	0.05639191326808657
1104	7	-0.05743240903201041
1104	8	-0.0252292788717412
1104	9	0.010252694903805093
1104	10	0.028051307201160114
1104	11	0.044992824797311926
1104	12	0.01831927090267982
1104	13	0.0626381381781087
1104	14	0.05021548932305819
1104	15	-0.03154751829142555
1104	16	0.05465053201031367
1104	17	-0.006092540871449614
1104	18	0.07234525515359991
1104	19	0.05345187383644401
1104	20	0.0328835778541503
1104	21	0.055048627425414054
1104	22	0.08494379868510293
1104	23	0.014869655223492373
1104	24	0.04418718592293658
1104	25	0.05340960940820594
1104	26	0.02876004714017455
1104	27	0.0380611608481658
1104	28	0.07903164647307648
1104	29	0.03403909479101941
1104	30	0.05068550554194218
1104	31	0.05832410448090286
1104	32	-0.052875446549144556
1104	33	0.02526499477161792
1104	34	-0.006900631299352256
1104	35	-0.03469605094450401
1104	36	-0.02519326760820917
1104	37	-0.11165534790102782
1104	38	-0.009455679541886752
1104	39	1.1123919093640282
1104	40	-0.08024648311907612
1104	41	-0.03291232643413622
1104	42	-0.021377400703798344
1104	43	0.012030068857470155
1104	44	-0.03050995654464117
1104	45	-0.004308006261481978
1104	46	-0.05302379587519823
1104	47	0.07912550274537722
1104	48	0.01463592338195241
1104	49	-0.04972591883558408
1104	50	0.019939473184692247
1104	51	0.011012201370668606
1104	52	0.03592685408276708
1104	53	-0.052467949136016045
1104	54	0.035373954373071115
1104	55	0.02750250799785192
1104	56	0.03152484138838929
1104	57	-0.023224137466812067
1104	58	0.027267583829839737
1104	59	0.01985661186707688
1104	60	0.03874659219742682
1104	61	0.1250585492204621
1201	1	0.1263981343502678
1201	2	0.12832296949342492
1201	3	0.1213840699615858
1201	4	0.05717852968928128
1201	5	0.06583578578411675
1201	6	0.05574471611753751
1201	7	0.061224178926288175
1201	8	0.00931265405505144
1201	9	0.03941055989816533
1201	10	0.058048756504256005
1201	11	-0.0034976592709554666
1201	12	0.0434699139049215
1201	13	-0.00416082881375246
1201	14	0.05327692833078146
1201	15	-0.014136359932685139
1201	16	0.06856738025541631
1201	17	0.03236090264170599
1201	18	0.07547805581398137
1201	19	0.01864209362550895
1201	20	-0.014592292987039457
1201	21	-0.047361612393139296
1201	22	0.10835512194712038
1201	23	0.0057781565661017
1201	24	-2.9233930991523494E-4
1201	25	0.04086008418017428
1201	26	0.08961695111486989
1201	27	0.04062909545388749
1201	28	0.05262614738269633
1201	29	0.04498744293751855
1201	30	0.11529480290450067
1201	31	0.0823684007802683
1201	32	0.06744439830676896
1201	33	0.16197547813591484
1201	34	0.09203036632209205
1201	35	0.08086052413976574
1201	36	0.09091250289753816
1201	37	0.13295853492989096
1201	38	0.10870740401441026
1201	39	1.3657203518525662
1201	40	0.16701742432678657
1201	41	0.15207527930044762
1201	42	0.13000899226842932
1201	43	0.18114018904881846
1201	44	0.10121075612682517
1201	45	0.09333811998210706
1201	46	0.17309837816719664
1201	47	0.0543519192862334
1201	48	0.1347489158798183
1201	49	0.1465471947456436
1201	50	0.061309944793599414
1201	51	0.09104574134741483
1201	52	0.09098622649560184
1201	53	0.17584280964568677
1201	54	0.19958603734738745
1201	55	0.16056450632663655
1201	56	0.13810057659422625
1201	57	0.12075342122211907
1201	58	0.17956986504007233
1201	59	0.1639935288411678
1201	60	0.08802482694158442
1201	61	0.2723432142281297
1202	1	0.05436840991229985
1202	2	0.07740930690174253
1202	3	0.07539169439524329
1202	4	-0.032529453920318474
1202	5	-0.04686662921744755
1202	6	0.08420128189548137
1202	7	0.05485998624363475
1202	8	0.04892036589642146
1202	9	0.02681166296029147
1202	10	0.02316270874300841
1202	11	-7.863181262734997E-5
1202	12	0.04685139093059831
1202	13	0.02237073220376118
1202	14	0.06360584104952238
1202	15	-0.08507845425840088
1202	16	-0.01927919206841011
1202	17	0.039198836427935646
1202	18	-0.050680859555093605
1202	19	-0.07821016262251845
1202	20	-0.015396962419679028
1202	21	-0.0237784871215041
1202	22	0.011388397444711984
1202	23	0.0035359112149677685
1202	24	0.033657127982692714
1202	25	-0.06992338258236686
1202	26	0.028927336207878795
1202	27	-0.016562272694912394
1202	28	0.06777883713230182
1202	29	-0.040741527245501175
1202	30	0.04482015090010805
1202	31	0.00430202033673055
1202	32	0.08538987120238062
1202	33	3.4060982164874076E-4
1202	34	-0.012491747491090166
1202	35	0.029215960546046915
1202	36	-0.22997447708348062
1202	37	-0.17257903948658537
1202	38	-0.22470046490889928
1202	39	1.095605432992997
1202	40	-0.22210014535107092
1202	41	-0.1549471119014143
1202	42	-0.13938592732761634
1202	43	-0.11823596502133427
1202	44	-0.10482640862654335
1202	45	-0.05064743933279914
1202	46	-0.014779033963577604
1202	47	-0.1042654689382374
1202	48	-0.019130092265477572
1202	49	-0.09221553545667405
1202	50	-0.11406171969836976
1202	51	0.03397247536583592
1202	52	-0.1045386340711616
1202	53	-0.13937805230875
1202	54	-0.014687148591214338
1202	55	-0.08362813756783538
1202	56	-0.02187686407488343
1202	57	-0.10313158530196631
1202	58	-0.05753725447350888
1202	59	-0.014585063729853687
1202	60	-0.01947951800686809
1202	61	-0.08639278491136082
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2403	1	7.830018018637475E-4
2403	2	0.059933114433285084
2403	3	0.1783123825957773
2403	4	0.025004001418082566
2403	5	0.0988494211493034
2403	6	0.06736199621969519
2403	7	0.087666810169992
2403	8	0.05257695577734722
2403	9	0.051923488001648366
2403	10	0.05916606487686238
2403	11	0.05732109309638389
2403	12	0.09200953231884057
2403	13	0.05756720641973345
2403	14	0.06051879281974237
2403	15	0.04782734580842174
2403	16	0.038184773439390085
2403	17	-0.003065603435445041
2403	18	0.010089372101319327
2403	19	0.010531475593367645
2403	20	0.015686488711516233
2403	21	0.03765539741345947
2403	22	0.012458615824947117
2403	23	0.03917669540895474
2403	24	0.019752162887428426
2403	25	-0.05146020955234576
2403	26	0.024666834705648455
2403	27	0.09783736063374704
2403	28	0.05869879094031205
2403	29	0.08247569068476679
2403	30	0.034716504505610146
2403	31	0.08431706063567646
2403	32	-0.028148513164680367
2403	33	-0.002160399171316385
2403	34	0.1060731512770019
2403	35	0.04896232078964147
2403	36	0.027320869350667465
2403	37	0.05129107352156126
2403	38	0.05506434325995713
2403	39	1.21014817037927
2403	40	0.07416787267616343
2403	41	0.01633594689344875
2403	42	0.1287514574419788
2403	43	-0.009659479511384461
2403	44	0.05026252007804288
2403	45	0.08370339926166537
2403	46	0.035537102584314084
2403	47	0.11444789872736294
2403	48	0.015012036247405547
2403	49	0.1094179567419502
2403	50	0.058776111565258304
2403	51	-0.004797100948984223
2403	52	0.11343346707415947
2403	53	0.04752372078489486
2403	54	0.0331268541283265
2403	55	0.11270538024891863
2403	56	0.09285984492485966
2403	57	0.05304505922221381
2403	58	0.044409201299856704
2403	59	0.04948855949584896
2403	60	0.0077488722883884975
2403	61	0.10794827448923172
2404	1	0.005001688062399978
2404	2	-0.07726565229293669
2404	3	-0.01563316334712539
2404	4	0.029326884691634802
2404	5	0.06879621021686688
2404	6	-0.06908450944104061
2404	7	-0.01702985726436168
2404	8	0.04568974005921689
2404	9	0.022000587973835195
2404	10	-0.033346015868325196
2404	11	0.021690756433379477
2404	12	-0.03640992503837026
2404	13	0.036768655249467486
2404	14	0.007618382993236139
2404	15	0.050180827969818154
2404	16	-0.029756850119426304
2404	17	0.026031347812960348
2404	18	-0.015364174161931032
2404	19	-0.04449307954453019
2404	20	-0.06668137936006957
2404	21	-0.06700054786320209
2404	22	-0.004351749425019591
2404	23	-0.07260743733804276
2404	24	0.015877481901902968
2404	25	-0.036782521875387886
2404	26	0.020680458659015244
2404	27	-0.07079477497905629
2404	28	-0.05457008460391677
2404	29	-0.05810042959633677
2404	30	-0.06205201129390758
2404	31	-0.026106822767609117
2404	32	-0.0516396392570968
2404	33	-0.11415163317744259
2404	34	0.023766948187073922
2404	35	0.014083081701905087
2404	36	0.06503773180796202
2404	37	0.05939271447584815
2404	38	-0.0245167071816752
2404	39	1.2686229911258238
2404	40	0.054346136183816895
2404	41	0.06986373650183708
2404	42	0.016866101045856396
2404	43	-0.0022670504844484185
2404	44	-0.009245350707061561
2404	45	-0.07241307205607228
2404	46	-0.0537477148443557
2404	47	0.09162409891438728
2404	48	0.05403562749829405
2404	49	-0.04142221570540272
2404	50	0.040992422108040216
2404	51	0.05784107563431462
2404	52	-0.0058302271895698254
2404	53	-6.926066931285391E-4
2404	54	-0.008067163489371865
2404	55	0.04939548197868504
2404	56	-0.02159294655642441
2404	57	0.024005377547965168
2404	58	-0.012802844728078355
2404	59	-0.040231403275051036
2404	60	0.02656031143982318
2404	61	0.09722360947260711
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	9.0
17	62.0
18	168.0
19	248.0
20	302.0
21	435.0
22	582.0
23	795.0
24	1350.0
25	1757.0
26	2372.0
27	3013.0
28	4056.0
29	5089.0
30	6260.0
31	7768.0
32	10850.0
33	12894.0
34	16592.0
35	21486.0
36	32065.0
37	48481.0
38	123815.0
39	790843.0
40	709694.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	32.534622701120384	14.138644053868271	20.39577209373088	32.930961151280464
2	30.94436668221911	18.5663978315448	24.80609433668822	25.68314114954787
3	27.139966662705874	19.710925015519276	19.775389703195916	33.373718618578934
4	27.486014597567443	19.663461938883444	20.507333486952973	32.34318997659614
5	27.596549889893645	21.283119357951698	20.3562937191072	30.764037033047455
6	29.271299166123445	24.340500148252126	22.131432448669784	24.256768236954645
7	28.360131616792135	23.614619991493548	21.760746613244077	26.26450177847024
8	26.128687285742362	26.299204991043794	22.321161852451933	25.250945870761903
9	25.245448881890255	25.709861153834623	23.598073499738476	25.446616464536646
10	26.68671494392516	23.771145361485317	22.93787958373913	26.60426011085039
11	25.674380589299417	24.697138123228054	23.168864166628726	26.459617120843802
12	26.67588754160221	23.889025233955177	23.13510488143717	26.29998234300544
13	26.071551916561265	22.34337190849901	22.94143319270666	28.643642982233064
14	25.963444468752115	24.029670413873287	23.20190162499875	26.804983492375843
15	26.057448530971367	23.214339256385113	23.084521478789952	27.643690733853564
16	26.613366233829687	23.688301852429724	22.354254835962077	27.344077077778508
17	26.544515060083757	22.984798327138577	22.58607229595344	27.88461431682423
18	27.321811496591312	23.59535276787271	22.609392854802866	26.47344288073311
19	26.672944709175972	22.473578362074996	23.274639558552927	27.5788373701961
20	26.44534715983356	23.369309922453592	22.565916670090715	27.619426247622137
21	26.120747190705533	23.26508923445268	23.75021238366095	26.863951191180828
22	27.6761174156823	22.592735312767562	22.298785220984506	27.432362050565633
23	26.44745711515803	23.865760200245862	22.58140818418355	27.105374500412548
24	26.441349349745085	24.315402784918927	22.680964760414575	26.562283104921413
25	26.77644357035535	22.4575871217211	22.253143555807764	28.512825752115784
26	26.832857112714926	23.282857279290344	23.29423993301447	26.59004567498026
27	26.50875686984796	23.50329208555758	22.468025848063228	27.51992519653123
28	26.909315230656983	23.17452773092073	21.99978234145074	27.916374696971545
29	26.679552200849976	24.352826729358252	22.290012248845912	26.67760882094586
30	27.537193515107834	24.038943112272943	22.348646796810193	26.07521657580903
31	26.8594536548313	23.092905774947724	23.007174958606008	27.040465611614973
32	26.85556689502306	23.837164753085254	22.166579862364284	27.140688489527403
33	26.629979111303104	24.76521038060516	22.244907477842546	26.35990303024919
34	27.100654863502548	23.64415936603616	21.78928653526457	27.465899235196723
35	27.8650694675028	24.028393335650584	21.91827143575797	26.188265761088648
36	26.940076158282185	25.6084167228396	21.780235937425388	25.671271181452827
37	27.280167641503045	23.813844194235827	21.351803956277283	27.55418420798385
38	27.311872496510244	24.66287911177544	21.378733649234363	26.646514742479955
39	28.409266710136837	24.6545209934577	21.448462061196683	25.48775023520878
40	27.533140179879247	25.53145887863648	20.964127427975566	25.971273513508713
41	27.384721480344652	24.925735125092586	22.05580720782949	25.63373618673327
42	27.414038754326796	26.39481928232646	21.010046718852895	25.18109524449385
43	27.558626219193265	25.941734138966098	20.59871648086104	25.900923160979595
44	27.458181240720357	25.689927628532374	21.545808795848494	25.306082334898772
45	27.421701223663035	25.749006377617594	20.629477408486242	26.19981499023313
46	27.74896639951671	25.483263057014327	21.17973154705256	25.588038996416408
47	28.553692255242407	25.920468010301022	20.211928354801202	25.31391137965537
48	28.486118159719176	26.386379461028568	20.09826839298029	25.029233986271965
49	28.764909888250102	25.91608152423173	19.71497835074787	25.604030236770303
50	28.664575960057437	26.535964188505627	19.73602237885247	25.06343747258446
51	28.522376076216027	26.5697789988373	19.795434278778405	25.112410646168264
52	28.691283552454045	26.342514600335594	19.319639353109906	25.646562494100454
53	28.41843301391571	26.939520906881008	19.38543664414937	25.25660943505391
54	28.397500036091337	27.079444259977592	19.26544681635504	25.257608887576026
55	28.61610251273469	26.68727019532634	19.018193367410962	25.67843392452801
56	28.33086986795011	27.316425557999896	18.995927786223767	25.356776787826224
57	28.16257316825339	27.312705373612012	18.981546774933285	25.543174683201315
58	28.196332453444946	27.078056131474646	18.72585350469132	25.999757910389086
59	28.108325106358407	27.517815241206762	18.643121045915958	25.730738606518873
60	27.945025669272276	27.830532830349597	18.63440359891748	25.590037901460644
61	28.252246269543463	27.301822446148943	18.422019937967313	26.023911346340284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	1.0
3	2.0
4	1.5
5	1.0
6	2.5
7	4.0
8	4.0
9	6.5
10	9.0
11	17.5
12	41.0
13	56.0
14	55.5
15	55.0
16	90.5
17	151.0
18	176.0
19	233.5
20	423.0
21	555.0
22	727.5
23	900.0
24	1224.5
25	2000.0
26	2451.0
27	3023.0
28	3595.0
29	4426.5
30	6365.0
31	7472.0
32	9026.0
33	10580.0
34	12357.5
35	16876.5
36	19618.0
37	22907.5
38	26197.0
39	30281.5
40	39169.5
41	43973.0
42	49458.5
43	61159.0
44	67374.0
45	73815.0
46	80256.0
47	86517.5
48	99109.0
49	105439.0
50	110029.5
51	114620.0
52	117463.0
53	122344.0
54	124382.0
55	125231.5
56	126081.0
57	124234.5
58	118870.0
59	115352.0
60	110924.5
61	105911.0
62	105325.0
63	91047.0
64	76769.0
65	68821.0
66	54330.5
67	47788.0
68	41831.0
69	35874.0
70	30897.0
71	21778.5
72	17637.0
73	14703.5
74	11770.0
75	9497.5
76	5849.0
77	4473.0
78	3518.0
79	2563.0
80	1991.5
81	1096.0
82	772.0
83	573.0
84	273.5
85	173.0
86	136.5
87	100.0
88	71.5
89	33.5
90	24.0
91	18.0
92	12.0
93	11.5
94	9.0
95	7.0
96	7.5
97	8.0
98	6.5
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03475873771367462
3	0.0
4	5.5525140117691085E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	6.66301681412293E-4
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.0943050084786887
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1800986.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.75499638469479
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.80504189536956	65.21310775104331
2	12.271304925684174	19.32853150115489
3	3.0898710284434863	7.300283450227199
4	1.0734040152729027	3.381437173685373
5	0.40528491223652924	1.5959105898979604
6	0.1786351173983929	0.8441044814933978
7	0.07925941502946772	0.43694524608691276
8	0.03277185328635886	0.2064757749669552
9	0.017201953538070337	0.12192658098303757
>10	0.045404192049710926	0.5239573614175532
>50	7.98619249038944E-4	0.040472598129921974
>100	8.648305280437058E-4	0.09925444486405233
>500	7.862095709488235E-5	0.031020883890761207
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	7.862095709488235E-5	0.8765721621586955
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCGATAATCTGGGGGGGGGGGGGGG	14157	0.7860694086461527	TruSeq Adapter, Index 18 (97% over 38bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.9368756892058017	0.0	0.0	0.0	1.1105028023538217E-4
2	0.9383193428488616	0.0	0.0	0.0	2.2210056047076434E-4
3	0.9402071976128632	0.0	0.0	0.0	2.2210056047076434E-4
4	0.941317700415217	0.0	0.0	0.0	2.2210056047076434E-4
5	0.943483180879807	0.0	0.0	0.0	2.2210056047076434E-4
6	0.9489246446113406	0.0	0.0	0.0	2.2210056047076434E-4
7	0.9538663820818152	0.0	0.0	0.0	2.2210056047076434E-4
8	0.9952881366096127	0.0	5.5525140117691085E-5	0.0	2.2210056047076434E-4
9	0.9998411980992634	0.0	5.5525140117691085E-5	0.0	2.2210056047076434E-4
10	1.003339281926678	0.0	5.5525140117691085E-5	0.0	2.7762570058845545E-4
11	1.009280471919271	0.0	5.5525140117691085E-5	0.0	4.442011209415287E-4
12	1.0190528965799845	5.5525140117691085E-5	5.5525140117691085E-5	0.0	4.997262610592197E-4
13	1.0388753716020003	5.5525140117691085E-5	5.5525140117691085E-5	0.0	4.997262610592197E-4
14	1.0611409527891944	5.5525140117691085E-5	5.5525140117691085E-5	0.0	4.997262610592197E-4
15	1.0925126569556898	5.5525140117691085E-5	5.5525140117691085E-5	0.0	4.997262610592197E-4
16	1.1289371488728952	5.5525140117691085E-5	5.5525140117691085E-5	0.0	4.997262610592197E-4
17	1.1729130598461066	5.5525140117691085E-5	5.5525140117691085E-5	0.0	4.997262610592197E-4
18	1.2207202054874386	5.5525140117691085E-5	5.5525140117691085E-5	0.0	4.997262610592197E-4
19	1.286795122227491	5.5525140117691085E-5	5.5525140117691085E-5	5.5525140117691085E-5	4.997262610592197E-4
20	1.3711933352063814	5.5525140117691085E-5	5.5525140117691085E-5	5.5525140117691085E-5	4.997262610592197E-4
21	1.5580354317024119	5.5525140117691085E-5	5.5525140117691085E-5	5.5525140117691085E-5	4.997262610592197E-4
22	1.6937943992901665	5.5525140117691085E-5	5.5525140117691085E-5	5.5525140117691085E-5	4.997262610592197E-4
23	1.8506529201226438	5.5525140117691085E-5	5.5525140117691085E-5	5.5525140117691085E-5	4.997262610592197E-4
24	2.037161865777968	5.5525140117691085E-5	5.5525140117691085E-5	5.5525140117691085E-5	4.997262610592197E-4
25	2.2650370408209723	5.5525140117691085E-5	5.5525140117691085E-5	5.5525140117691085E-5	4.997262610592197E-4
26	2.528226204978828	5.5525140117691085E-5	5.5525140117691085E-5	5.5525140117691085E-5	6.10776541294602E-4
27	2.8362241572116607	5.5525140117691085E-5	5.5525140117691085E-5	5.5525140117691085E-5	6.10776541294602E-4
28	3.1818681544442877	2.2210056047076434E-4	5.5525140117691085E-5	5.5525140117691085E-5	6.10776541294602E-4
29	3.57404221909554	2.2210056047076434E-4	5.5525140117691085E-5	1.1105028023538217E-4	6.66301681412293E-4
30	4.019853569100482	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
31	4.523300014547587	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
32	5.063004376491544	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
33	5.67222621386285	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
34	6.3506323758208	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
35	7.0711821191280775	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
36	7.84403654442622	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
37	8.700123154760782	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
38	9.616176916422448	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
39	10.566489689536732	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
40	11.6325723797964	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
41	12.720587500402557	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
42	13.878064571295946	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
43	15.084070614652196	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
44	16.3272785018873	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
45	17.606300104498313	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
46	18.93673798685831	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
47	20.264399612212422	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
48	21.659080081688586	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
49	23.069918366939	2.7762570058845545E-4	5.5525140117691085E-5	1.1105028023538217E-4	7.773519616476752E-4
50	24.506575842344137	2.7762570058845545E-4	1.1105028023538217E-4	1.1105028023538217E-4	7.773519616476752E-4
>>END_MODULE