##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-37A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1028086 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.57954879261074 40.0 40.0 40.0 40.0 40.0 2 39.33584544483633 40.0 40.0 40.0 40.0 40.0 3 39.30034646906971 40.0 40.0 40.0 40.0 40.0 4 39.24057325943549 40.0 40.0 40.0 40.0 40.0 5 39.26899889697943 40.0 40.0 40.0 40.0 40.0 6 39.381950537211864 40.0 40.0 40.0 40.0 40.0 7 39.415076170670545 40.0 40.0 40.0 40.0 40.0 8 39.405948529597715 40.0 40.0 40.0 40.0 40.0 9 39.421285767922136 40.0 40.0 40.0 40.0 40.0 10-11 39.43195997222023 40.0 40.0 40.0 40.0 40.0 12-13 39.34749038504561 40.0 40.0 40.0 40.0 40.0 14-15 39.37742173320131 40.0 40.0 40.0 40.0 40.0 16-17 39.48114068278335 40.0 40.0 40.0 40.0 40.0 18-19 39.5172310487644 40.0 40.0 40.0 40.0 40.0 20-21 39.49552663882204 40.0 40.0 40.0 40.0 40.0 22-23 39.53410123277625 40.0 40.0 40.0 40.0 40.0 24-25 39.51054094696358 40.0 40.0 40.0 40.0 40.0 26-27 39.48703513130225 40.0 40.0 40.0 40.0 40.0 28-29 39.50440916421389 40.0 40.0 40.0 40.0 40.0 30-31 39.4854115168138 40.0 40.0 40.0 40.0 40.0 32-33 39.48499482383059 40.0 40.0 40.0 40.0 40.0 34-35 39.47874691194572 40.0 40.0 40.0 40.0 40.0 36-37 39.461811635747644 40.0 40.0 40.0 40.0 40.0 38-39 39.401883258107816 40.0 40.0 40.0 40.0 40.0 40-41 39.45469934413957 40.0 40.0 40.0 40.0 40.0 42-43 39.42525984338016 40.0 40.0 40.0 40.0 40.0 44-45 39.32712561824226 40.0 40.0 40.0 40.0 40.0 46-47 39.31547810600059 40.0 40.0 40.0 40.0 40.0 48-49 39.39062361185002 40.0 40.0 40.0 40.0 40.0 50-51 39.354123916251396 40.0 40.0 40.0 40.0 40.0 52-53 39.31221052109367 40.0 40.0 40.0 40.0 40.0 54-55 39.22067636493266 40.0 40.0 40.0 40.0 40.0 56-57 39.2345598563075 40.0 40.0 40.0 40.0 40.0 58-59 39.17008904006936 40.0 40.0 40.0 40.0 40.0 60-61 39.100094087523075 40.0 40.0 40.0 40.0 40.0 62-63 39.41512431563632 40.0 40.0 40.0 40.0 40.0 64-65 39.41728981001515 40.0 40.0 40.0 40.0 40.0 66-67 39.41891022723115 40.0 40.0 40.0 40.0 40.0 68-69 39.413785756535525 40.0 40.0 40.0 40.0 40.0 70-71 39.4168257541261 40.0 40.0 40.0 40.0 40.0 72-73 39.41568693216225 40.0 40.0 40.0 40.0 40.0 74-75 39.41742629496295 40.0 40.0 40.0 40.0 40.0 76-77 39.41645398437049 40.0 40.0 40.0 40.0 40.0 78-79 39.4021045682059 40.0 40.0 40.0 40.0 40.0 80-81 39.39446038543417 40.0 40.0 40.0 40.0 40.0 82-83 39.40135631497667 40.0 40.0 40.0 40.0 40.0 84-85 39.38291394121909 40.0 40.0 40.0 40.0 40.0 86-87 39.38113876042842 40.0 40.0 40.0 40.0 40.0 88-89 39.415597538607514 40.0 40.0 40.0 40.0 40.0 90-91 39.47859076363509 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.004143780490487359 1101 2 0.04062089984873296 1101 3 -0.03669926449802574 1101 4 -0.05076701811344719 1101 5 -0.012059912659886152 1101 6 0.10099800542233339 1101 7 -0.04228272716001413 1101 8 0.08045160453865208 1101 9 -0.11191834152712943 1101 10-11 -0.009242256858350117 1101 12-13 0.030620455343040476 1101 14-15 -0.035956896675799044 1101 16-17 0.052583011005765457 1101 18-19 0.06528065992677767 1101 20-21 0.03314574657949265 1101 22-23 -0.003367607096294023 1101 24-25 0.0017220343211405975 1101 26-27 -0.005284218350702474 1101 28-29 0.023947595051836856 1101 30-31 0.020228134721357094 1101 32-33 0.08968294781643493 1101 34-35 0.047274283632425806 1101 36-37 -0.054186559537342305 1101 38-39 0.08512317929726976 1101 40-41 0.010049697329336027 1101 42-43 -0.012162229078441555 1101 44-45 0.04900690674422492 1101 46-47 0.036242790762067045 1101 48-49 0.030873220081183206 1101 50-51 -0.010261740298119548 1101 52-53 0.06327124922191985 1101 54-55 0.0051955067895193 1101 56-57 0.0973920005654918 1101 58-59 -0.014214337239010888 1101 60-61 0.10772621192718645 1101 62-63 0.020245924192046516 1101 64-65 -0.02858530390287939 1101 66-67 -0.12931474005241483 1101 68-69 -0.01948976346191955 1101 70-71 -9.538647126277056E-4 1101 72-73 0.010753227758272033 1101 74-75 0.19879502075756506 1101 76-77 0.11816460444210009 1101 78-79 0.01827726502121152 1101 80-81 -0.03431861302589567 1101 82-83 -0.048818015839124485 1101 84-85 -0.06474771180226213 1101 86-87 0.07957448000166067 1101 88-89 -0.08234014727594996 1101 90-91 0.10445146905033909 1102 1 -0.010895990692240787 1102 2 0.05159195493743596 1102 3 0.020971905235690258 1102 4 -0.026307445254765582 1102 5 -0.10830853869258306 1102 6 0.010753468789111764 1102 7 -0.01722629108347462 1102 8 -0.16386046628123552 1102 9 -0.061368163650072916 1102 10-11 -0.08175818306006022 1102 12-13 -0.19015393036060857 1102 14-15 -0.11722189212545686 1102 16-17 -0.0028691276556074286 1102 18-19 -0.06674430195671022 1102 20-21 0.011274550843815234 1102 22-23 -0.08758737095121205 1102 24-25 -0.013039988631497579 1102 26-27 -0.09902129123893388 1102 28-29 -0.005576450853446602 1102 30-31 -8.364092396888623E-4 1102 32-33 -0.04154610216133392 1102 34-35 -0.05875552962780972 1102 36-37 -0.048544081124582306 1102 38-39 -0.06324107374344834 1102 40-41 -0.14095336229691924 1102 42-43 -0.007146629835844465 1102 44-45 0.022855263785267255 1102 46-47 -0.08622170383252836 1102 48-49 -0.15285394539734654 1102 50-51 -0.09476181670780903 1102 52-53 -0.09497329264296894 1102 54-55 -0.021194877923825572 1102 56-57 -0.1257568821193047 1102 58-59 -0.05213060163242744 1102 60-61 -0.03388932999908434 1102 62-63 -0.07028675793128514 1102 64-65 -0.0850052729949411 1102 66-67 0.006688728782982878 1102 68-69 -0.04735347429372894 1102 70-71 -0.09040465663351682 1102 72-73 0.05680385410304467 1102 74-75 0.11419375932390352 1102 76-77 0.09616187331900505 1102 78-79 -0.05478952184198249 1102 80-81 -0.03881811087632059 1102 82-83 0.19561547143493385 1102 84-85 0.08827216627197032 1102 86-87 0.10853061226806204 1102 88-89 0.09145350774938521 1102 90-91 -0.0015893827532238447 1103 1 0.018820498413859355 1103 2 0.12035125782177403 1103 3 0.005636063786141676 1103 4 -0.06620876142977039 1103 5 0.050083688491177725 1103 6 0.09281223371768021 1103 7 -0.004931833551509612 1103 8 0.002519056239073336 1103 9 0.08359860967013333 1103 10-11 -0.05255152131586982 1103 12-13 0.037920563035079624 1103 14-15 -0.0032461763161251156 1103 16-17 0.009088375010733785 1103 18-19 -0.042852736962508686 1103 20-21 0.018177730525728464 1103 22-23 0.029037103106190898 1103 24-25 0.08180548093707785 1103 26-27 -0.08179264245723772 1103 28-29 0.04046037391830737 1103 30-31 -0.029395531633419125 1103 32-33 0.07942019513937737 1103 34-35 0.019381473297634955 1103 36-37 0.08121901630131134 1103 38-39 0.05146615990446435 1103 40-41 0.020064078474483438 1103 42-43 0.007487087078416721 1103 44-45 0.04078050458438298 1103 46-47 0.07082932390905938 1103 48-49 0.06978613344976026 1103 50-51 0.10348222916666572 1103 52-53 0.0800868294503303 1103 54-55 0.12060697823513777 1103 56-57 0.08370851449123506 1103 58-59 0.11682180366300088 1103 60-61 0.19903221921255465 1103 62-63 -0.015285826109185052 1103 64-65 0.12849935568605275 1103 66-67 0.0782340891216009 1103 68-69 0.03225788353564951 1103 70-71 0.02353417565328897 1103 72-73 0.05602116827083847 1103 74-75 0.028398029719134854 1103 76-77 -0.07404462695613034 1103 78-79 -0.21407423082642651 1103 80-81 -0.09027169232587084 1103 82-83 0.014272426644524216 1103 84-85 0.03569481335524216 1103 86-87 -0.05455288735046082 1103 88-89 -0.2274044425865398 1103 90-91 -0.1546701328488993 1104 1 -0.014746812335069137 1104 2 -0.020410357281086533 1104 3 0.029217092427607838 1104 4 0.01907128118447332 1104 5 -0.05338759700995155 1104 6 -0.10957679884155169 1104 7 -0.1171808519632691 1104 8 -0.09730653464698236 1104 9 -0.08508278296802274 1104 10-11 0.03484205046250821 1104 12-13 -0.0061542524607034466 1104 14-15 0.05213229718886225 1104 16-17 -0.023191630373332828 1104 18-19 -0.028460405235797737 1104 20-21 -0.08595653871726938 1104 22-23 0.024539409945958823 1104 24-25 0.01881916159943131 1104 26-27 -0.02415315096433801 1104 28-29 -0.08799093615474618 1104 30-31 -0.03796563464648273 1104 32-33 -0.09241059862478096 1104 34-35 -0.08904272115806577 1104 36-37 -0.18289404356320205 1104 38-39 -0.125163977113111 1104 40-41 -0.12249997380941835 1104 42-43 -0.06136567235945023 1104 44-45 -0.1432866570515614 1104 46-47 -0.12819153319587429 1104 48-49 -0.14926808081612108 1104 50-51 -0.1630920955870181 1104 52-53 -0.13749720686325162 1104 54-55 -0.15801264915725 1104 56-57 -0.18402630452423097 1104 58-59 -0.19712245206932266 1104 60-61 -0.1486671612354229 1104 62-63 -0.16030369738320616 1104 64-65 -0.05487963461965961 1104 66-67 0.010437997705416535 1104 68-69 -0.05058298057679167 1104 70-71 0.03310606244797043 1104 72-73 -0.010368858862911168 1104 74-75 -0.1278223539056853 1104 76-77 -0.0927030671228124 1104 78-79 -0.21090923561226305 1104 80-81 -0.19206854939931617 1104 82-83 -0.12316828556257065 1104 84-85 -0.09483826236347426 1104 86-87 -0.21076514485523745 1104 88-89 -0.1653571302929251 1104 90-91 0.38623113006728715 1201 1 0.03203169026567565 1201 2 -0.03852119750355598 1201 3 -0.083766972625547 1201 4 -0.07850418243038604 1201 5 -0.06838309921398178 1201 6 0.12193113444434545 1201 7 -0.014334811911162149 1201 8 -0.04570331788597315 1201 9 -0.0927692776856972 1201 10-11 -0.02089429536137999 1201 12-13 -0.006052402132254997 1201 14-15 -0.03814270582034851 1201 16-17 0.07973987848586717 1201 18-19 -0.02419408728186312 1201 20-21 0.069216353995877 1201 22-23 -0.06865648954557457 1201 24-25 0.010386239299833733 1201 26-27 0.08366218798001768 1201 28-29 0.06295481180770679 1201 30-31 0.02958606151759824 1201 32-33 -0.02957908358007444 1201 34-35 0.00769403951325387 1201 36-37 0.01607233518685547 1201 38-39 0.029667484996359406 1201 40-41 0.06303870299250747 1201 42-43 0.015684293341983846 1201 44-45 0.013619515247548009 1201 46-47 0.09929781717315933 1201 48-49 -0.014201296348758774 1201 50-51 0.01485289125996303 1201 52-53 0.05819861777619195 1201 54-55 0.008522675200360652 1201 56-57 -0.00422430822331421 1201 58-59 -0.021250633238409478 1201 60-61 0.021631321904393985 1201 62-63 0.013577330215682082 1201 64-65 0.0040335747028663604 1201 66-67 0.03773892033693471 1201 68-69 0.030538532316413125 1201 70-71 0.004562553363257393 1201 72-73 -0.013762314499750516 1201 74-75 0.02782145061181751 1201 76-77 -0.06947259818355178 1201 78-79 -0.11307361127044402 1201 80-81 0.05620020482066934 1201 82-83 0.12326363252822148 1201 84-85 0.0672439416785906 1201 86-87 -0.1790231705248928 1201 88-89 0.10243972994832262 1201 90-91 -0.605651986815829 1202 1 0.12543321497766868 1202 2 0.07451855866059987 1202 3 -0.03161975599984146 1202 4 0.01975339500446438 1202 5 0.06246464360710746 1202 6 -0.019617636413435946 1202 7 0.00717056443292563 1202 8 0.05314466109572891 1202 9 0.08466114718201823 1202 10-11 -0.03593477476743345 1202 12-13 -0.013454561525904296 1202 14-15 -0.03179588919168452 1202 16-17 -0.0480230162529125 1202 18-19 -0.06279158840731469 1202 20-21 -0.003816317349716769 1202 22-23 -0.05213262625305504 1202 24-25 -0.057967499786961696 1202 26-27 -0.06476476089857641 1202 28-29 -0.02302241345262246 1202 30-31 -0.06211067872785492 1202 32-33 0.028102468652669188 1202 34-35 -0.0691464626830296 1202 36-37 -0.18114643374196504 1202 38-39 -0.1147639686688251 1202 40-41 -0.21453160370983682 1202 42-43 -0.10320530729556765 1202 44-45 -0.050797298978565664 1202 46-47 -0.012491040025729205 1202 48-49 -0.1176096928460666 1202 50-51 -0.06878432087488306 1202 52-53 -0.13495579995494467 1202 54-55 0.011679867873787941 1202 56-57 -0.058317781621511244 1202 58-59 -0.08992711978672219 1202 60-61 7.21945151134662E-4 1202 62-63 -0.07067258227413475 1202 64-65 -0.13887847777522921 1202 66-67 -0.07720095026785856 1202 68-69 -0.21316179774923683 1202 70-71 -0.19818308126491502 1202 72-73 -0.2427708541688247 1202 74-75 -0.25559942510141553 1202 76-77 -0.11587037037315895 1202 78-79 -0.24428889576815038 1202 80-81 -0.03393599212164844 1202 82-83 -0.1828737638347917 1202 84-85 -0.023964831824123678 1202 86-87 0.1615546669415906 1202 88-89 0.04265985272405004 1202 90-91 0.1130829819191348 1203 1 0.007784506029608451 1203 2 -0.021292512200915326 1203 3 -0.01940773435856613 1203 4 -0.10677287393305335 1203 5 -0.049568966569452755 1203 6 -0.08019898848807827 1203 7 0.042856597657852546 1203 8 0.08585904004991107 1203 9 -0.04237473521602908 1203 10-11 -0.05254677097478577 1203 12-13 -0.007779618237940156 1203 14-15 -0.012584396800356501 1203 16-17 -0.002201285586906465 1203 18-19 -0.042400504492214 1203 20-21 -0.004169774025100992 1203 22-23 0.0027373147813989362 1203 24-25 0.04421888221929038 1203 26-27 0.03565574033664376 1203 28-29 0.0013118630552284571 1203 30-31 0.05114460114811692 1203 32-33 -0.037000815382832286 1203 34-35 0.012818421633419064 1203 36-37 0.06563597140674204 1203 38-39 0.06436218635073487 1203 40-41 0.09143695994693246 1203 42-43 0.024602870772142182 1203 44-45 0.027191071042615533 1203 46-47 0.07938036473057508 1203 48-49 -0.008929762450414103 1203 50-51 0.07067302315081037 1203 52-53 0.0636874249315369 1203 54-55 0.02669222317597786 1203 56-57 0.05376546583079289 1203 58-59 0.07825941935988112 1203 60-61 0.036903352337368744 1203 62-63 0.007507565257228066 1203 64-65 -0.11094492902419262 1203 66-67 -0.09803013588593501 1203 68-69 -0.03144590990360996 1203 70-71 -0.0065007236916869715 1203 72-73 -0.006399282112887761 1203 74-75 -0.058045647782542176 1203 76-77 -0.14248172216147026 1203 78-79 -0.1433487039956276 1203 80-81 -0.17562125382174543 1203 82-83 -0.5270872894172314 1203 84-85 -0.0953839076377605 1203 86-87 -0.38299008901133647 1203 88-89 0.1464423322629429 1203 90-91 -0.3926649738288148 1204 1 0.005192447389347876 1204 2 -0.05259816495355096 1204 3 -0.1526388110399921 1204 4 -0.19420599845141595 1204 5 -0.13078004420953704 1204 6 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22-23 0.06683347688583297 2401 24-25 0.12741217549774575 2401 26-27 0.023228278044854278 2401 28-29 0.05203638507590824 2401 30-31 0.022068903513023486 2401 32-33 0.06587585640617277 2401 34-35 -0.00642186056128935 2401 36-37 0.04491967395610885 2401 38-39 0.17409649433620444 2401 40-41 0.02912505447001479 2401 42-43 0.060449281106308206 2401 44-45 0.024452752974276848 2401 46-47 0.04458411726327682 2401 48-49 0.07127360779309555 2401 50-51 0.017519445699562652 2401 52-53 0.035816443234928386 2401 54-55 0.016721087380517474 2401 56-57 -0.020400710771284025 2401 58-59 0.0896730607477707 2401 60-61 0.02468292559353813 2401 62-63 0.07915617531519814 2401 64-65 -0.026981277799059455 2401 66-67 0.14157626236266196 2401 68-69 0.13666904144761816 2401 70-71 0.10197721393649317 2401 72-73 0.08483857726407251 2401 74-75 0.16044905084815042 2401 76-77 0.07254708717776026 2401 78-79 0.15629406932769996 2401 80-81 0.10735973613797967 2401 82-83 0.24449322672040807 2401 84-85 0.16745135676804068 2401 86-87 0.1490856190277654 2401 88-89 0.22487485993906375 2401 90-91 0.39182814499265817 2402 1 0.002135358520462205 2402 2 0.06692395215232949 2402 3 0.039978418813078065 2402 4 0.12046158089639647 2402 5 0.1669818115885633 2402 6 0.09788677024473458 2402 7 0.0247882277651712 2402 8 0.11078279695926341 2402 9 0.09562804076689702 2402 10-11 0.11879914305578865 2402 12-13 0.10792478928406979 2402 14-15 0.03762161347715676 2402 16-17 0.028855487047337647 2402 18-19 0.01546968900314738 2402 20-21 0.04469924661376723 2402 22-23 0.07824746284617845 2402 24-25 0.01898948528711486 2402 26-27 0.07974746646834774 2402 28-29 0.07522630313679457 2402 30-31 -0.032746723052120785 2402 32-33 -0.02706570489515059 2402 34-35 0.06451472582126883 2402 36-37 0.06450562013022676 2402 38-39 -0.3072766136848699 2402 40-41 0.06926768314757936 2402 42-43 0.026438039920435585 2402 44-45 0.08187118827994055 2402 46-47 0.07369852788284703 2402 48-49 0.06801449638914647 2402 50-51 0.1161606224822549 2402 52-53 0.08830724270061552 2402 54-55 0.06804089976171213 2402 56-57 0.021212107075790243 2402 58-59 -0.030043009750123417 2402 60-61 0.03983924629281432 2402 62-63 0.13351610026108318 2402 64-65 0.10238580112157081 2402 66-67 0.13190891158126306 2402 68-69 0.1245177578349157 2402 70-71 0.01634384461039673 2402 72-73 0.1444530783518232 2402 74-75 0.11915888493017945 2402 76-77 0.13782992298799002 2402 78-79 0.15380953489767535 2402 80-81 0.1305133201161368 2402 82-83 0.21752501577149985 2402 84-85 -0.003078194615106611 2402 86-87 -0.040349050996660196 2402 88-89 0.19667150130091926 2402 90-91 0.28414393155151885 2403 1 -0.06446094040503425 2403 2 0.04084437352353376 2403 3 0.06367040056302642 2403 4 0.0572833522871008 2403 5 0.1370824576094023 2403 6 -0.050840758604181246 2403 7 0.0654236635081773 2403 8 0.0366190819338712 2403 9 0.08153404952803811 2403 10-11 0.032718178683346366 2403 12-13 0.05335876031502096 2403 14-15 0.06303854672425047 2403 16-17 -0.008796438241944315 2403 18-19 0.04009489389862608 2403 20-21 0.058962330702343024 2403 22-23 -0.014820774368885736 2403 24-25 0.0458826954863909 2403 26-27 0.07292987056127487 2403 28-29 0.01559420566338332 2403 30-31 0.0869149423065565 2403 32-33 -0.03724411555311491 2403 34-35 0.055980360723687284 2403 36-37 0.04266653750298843 2403 38-39 0.08406145882499061 2403 40-41 -0.013286135775437913 2403 42-43 0.09483761495548038 2403 44-45 0.025203667860182577 2403 46-47 -0.009710675063566043 2403 48-49 0.06813034784693173 2403 50-51 0.06843734506027488 2403 52-53 0.08090993009518144 2403 54-55 0.024787701954615216 2403 56-57 0.0880716603648466 2403 58-59 0.06266305548552964 2403 60-61 0.14647241096893993 2403 62-63 0.06307554512983415 2403 64-65 -0.02012613865446866 2403 66-67 0.0638933466399152 2403 68-69 0.004957922662534031 2403 70-71 -0.030273332404505027 2403 72-73 0.04847651280781662 2403 74-75 -0.09488596290180595 2403 76-77 0.004212595928549945 2403 78-79 0.10392416961055062 2403 80-81 -0.08031482577747795 2403 82-83 -0.02806533606095485 2403 84-85 -0.0825342123749877 2403 86-87 -0.30528626454835717 2403 88-89 -0.22756322972466592 2403 90-91 0.006655966668596136 2404 1 -0.014010978759351644 2404 2 -0.029029088405906123 2404 3 0.00616386785583245 2404 4 0.0623660791232723 2404 5 0.010647288881578731 2404 6 0.04140293787907012 2404 7 0.051874922340580554 2404 8 0.07547543721335614 2404 9 0.12480271061929926 2404 10-11 0.048915862456667014 2404 12-13 0.053089032786594714 2404 14-15 0.11610315036510599 2404 16-17 0.07343771643509456 2404 18-19 0.03322697619514514 2404 20-21 0.005038652679054678 2404 22-23 0.064907876619273 2404 24-25 0.05337114241711305 2404 26-27 0.05826635955077819 2404 28-29 0.03369401420361129 2404 30-31 0.039107686791957974 2404 32-33 0.023188025295119985 2404 34-35 0.0411813180174434 2404 36-37 0.05794310980778761 2404 38-39 0.13939632830025772 2404 40-41 0.025937571893805966 2404 42-43 0.07167600692743292 2404 44-45 0.019772025989119868 2404 46-47 0.06284293848438693 2404 48-49 0.07585494470496457 2404 50-51 -0.007178344996901842 2404 52-53 0.02953293731169282 2404 54-55 0.12533193137517884 2404 56-57 -0.03669223892067919 2404 58-59 0.021543376610850373 2404 60-61 -0.06247206669860361 2404 62-63 -0.009043763623402867 2404 64-65 0.03383179749646814 2404 66-67 0.05785298767639091 2404 68-69 0.1362098642489542 2404 70-71 0.08830686175437563 2404 72-73 0.06014281215384898 2404 74-75 0.07739629162852424 2404 76-77 0.09615693154908911 2404 78-79 0.1273676366660439 2404 80-81 0.1822747728133507 2404 82-83 0.19741461006272232 2404 84-85 0.1500382993376448 2404 86-87 0.1832192333396634 2404 88-89 0.06412320135880378 2404 90-91 0.3137002658697554 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 20.0 31 596.0 32 4110.0 33 7459.0 34 10124.0 35 12440.0 36 18060.0 37 26280.0 38 66408.0 39 448134.0 40 434455.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 32.82244870565303 13.13606060193408 20.865472343753343 33.17601834865955 2 30.310905025889294 18.673857164261292 25.347866103413185 25.667371706436228 3 27.262213472413787 19.08916180163916 20.110185334689902 33.53843939125715 4 27.47026999686797 19.844254274447856 20.013209011697462 32.67226671698671 5 27.786002338325783 21.486432068912524 20.636697708168384 30.090867884593315 6 28.416591608095043 24.55874314016532 22.574181537342206 24.450483714397432 7 27.4575278721819 23.849853027859538 22.182385520277485 26.510233579681074 8 26.233116684790964 25.431043706460354 22.754030304857764 25.581809303890918 9 25.246915141340313 25.002383069120675 24.02376843960525 25.726933349933763 10-11 25.735979285779592 23.91677349949323 23.490058224701045 26.857188990026128 12-13 25.872203298167662 23.283606624348547 23.527263283421814 27.316926794061974 14-15 26.081378406086653 23.180842847777324 23.554984699723562 27.18279404641246 16-17 26.60244376443216 22.94195232694541 22.892880556684947 27.562723351937485 18-19 26.56450919475608 23.026624231824965 22.92108831362357 27.487778259795387 20-21 26.340695233667223 23.27280986220997 23.138920284878893 27.247574619243913 22-23 26.595780897707 22.664786797991606 22.966026188470614 27.773406115830777 24-25 26.52793637886325 22.872697420254724 23.01670288283276 27.582663318049267 26-27 26.545979616491227 23.23244358934953 23.04510517602613 27.176471618133114 28-29 26.777672295897425 22.85776676270273 22.820561703981962 27.543999237417882 30-31 26.60916465644697 22.93216481207688 22.96791105750525 27.4907594739709 32-33 26.54894511591844 23.134242812014012 23.081403901322105 27.235408170745444 34-35 26.751327089464272 22.805741766833187 22.900813092880227 27.54211805082231 36-37 26.758290318035684 22.98397653478155 22.834782831452614 27.422950315730148 38-39 26.89034996795705 23.14348191651575 22.95196082867597 27.01420728685123 40-41 26.98137787671812 22.919940693598768 22.73249474891239 27.36618668077072 42-43 26.794316329952544 23.06778659207476 22.84526702908344 27.292630048889254 44-45 26.810892123374465 23.311017674444617 22.958134689789812 26.919955512391102 46-47 26.998855756674196 23.00749439879827 22.762603235601706 27.23104660892583 48-49 27.021912496365097 23.118043178505047 22.769283435852962 27.090760889276893 50-51 26.981920076864096 23.344056481192784 22.888545724367816 26.7854777175753 52-53 27.17902855789522 23.040943941123864 22.68088611129767 27.099141389683247 54-55 27.19067891351029 23.16533689074029 22.683271233772956 26.960712961976462 56-57 27.20703376614232 23.429857178635928 22.717870794235296 26.64523826098646 58-59 27.280637758141935 23.101843953634113 22.593211431843844 27.024306856380107 60-61 27.502133759962167 23.232489312726077 22.518372504609292 26.74700442270247 62-63 27.270696157129287 23.4903437303378 22.683316835452676 26.555643277080236 64-65 27.52511577378901 23.084741052898746 22.492224311784582 26.897918861527657 66-67 27.518143003824203 23.107728937684847 22.55052897283334 26.823599085657605 68-69 27.425498633296318 23.472334927820963 22.53793136584949 26.564235073033228 70-71 27.736744981210776 23.11559513136048 22.29435905545171 26.85330083197704 72-73 27.691825372880242 23.208666294727507 22.28213326771023 26.817375064682018 74-75 27.62965105070368 23.45652593021014 22.30884904569115 26.604973973395023 76-77 27.838342832562642 23.011210882890634 22.147318708104812 27.00312757644191 78-79 27.916199146394195 23.130913634084816 21.901434789874504 27.05145242964649 80-81 27.999794452134648 23.22016424559116 21.874146896066648 26.905894406207548 82-83 28.453837686702073 22.652113479507964 21.249286936679976 27.64476189710998 84-85 29.01847872346015 22.30844809196803 20.879328734231088 27.79374445034073 86-87 29.465873825063817 21.700689891269565 20.451889944853352 28.38154633881327 88-89 28.49977532456492 20.970112094825176 20.54140707545451 29.988705505155387 90-91 23.49552424000709 24.807232119117256 23.451209784631747 28.24603385624391 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.5 7 1.0 8 1.5 9 2.5 10 2.5 11 3.5 12 8.0 13 20.5 14 43.5 15 60.0 16 72.5 17 100.0 18 139.0 19 201.0 20 277.5 21 373.0 22 535.0 23 773.0 24 1057.5 25 1394.5 26 1810.5 27 2317.0 28 2993.5 29 3688.0 30 4425.5 31 5373.0 32 6583.5 33 7947.0 34 9454.0 35 11278.5 36 13530.5 37 15993.5 38 18773.0 39 22094.0 40 25596.0 41 29127.5 42 32687.5 43 36408.0 44 40351.5 45 44331.5 46 48117.0 47 51706.0 48 55003.5 49 57873.5 50 60300.5 51 62247.5 52 63351.0 53 64097.0 54 64970.5 55 65526.0 56 65556.5 57 65281.5 58 64786.0 59 64024.5 60 62932.5 61 61094.5 62 58670.0 63 55463.0 64 51755.5 65 47695.0 66 42886.5 67 38524.5 68 34310.5 69 29707.5 70 25484.0 71 21486.0 72 17656.0 73 14251.0 74 11305.5 75 8927.5 76 6946.5 77 5184.5 78 3819.0 79 2759.0 80 1940.0 81 1385.5 82 1007.0 83 719.0 84 513.5 85 366.5 86 252.0 87 171.0 88 120.0 89 74.5 90 42.5 91 30.0 92 21.0 93 13.0 94 8.0 95 5.5 96 3.5 97 2.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.024122495588890423 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.060156132507553695 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.1880186287906057E-4 66-67 0.0 68-69 0.0 70-71 1.4917980940787548E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.017242002675483E-4 84-85 5.254087680215207E-4 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 7560.0 32-33 17855.0 34-35 20818.0 36-37 24439.0 38-39 27366.0 40-41 30323.0 42-43 32943.0 44-45 35057.0 46-47 36450.0 48-49 38243.0 50-51 39661.0 52-53 40530.0 54-55 41590.0 56-57 41589.0 58-59 41892.0 60-61 42099.0 62-63 42244.0 64-65 41568.0 66-67 41229.0 68-69 39788.0 70-71 38659.0 72-73 37567.0 74-75 36586.0 76-77 35091.0 78-79 33264.0 80-81 31692.0 82-83 29665.0 84-85 28367.0 86-87 26537.0 88-89 24589.0 90-91 22825.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.83406798823505 #Duplication Level Percentage of deduplicated Percentage of total 1 99.83811233291253 99.67244894461038 2 0.15843767886323024 0.31634956007059756 3 0.0025798398185548706 0.007726677115330889 4 8.701484056749808E-4 0.0034748182036804147 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.726812737455816E-4 0.0 0.0 0.0 9.726812737455816E-5 2 9.726812737455816E-4 0.0 0.0 0.0 1.9453625474911632E-4 3 9.726812737455816E-4 0.0 0.0 0.0 1.9453625474911632E-4 4 0.0010699494011201397 0.0 0.0 0.0 1.9453625474911632E-4 5 0.0010699494011201397 0.0 0.0 0.0 1.9453625474911632E-4 6 0.001167217528494698 0.0 0.0 0.0 1.9453625474911632E-4 7 0.0016535581653674887 0.0 0.0 0.0 1.9453625474911632E-4 8 0.008462327081586559 0.0 9.726812737455816E-5 0.0 1.9453625474911632E-4 9 0.008656863336335675 0.0 9.726812737455816E-5 0.0 1.9453625474911632E-4 10-11 0.008754131463710235 0.0 9.726812737455816E-5 0.0 2.431703184363954E-4 12-13 0.008851399591084793 0.0 9.726812737455816E-5 0.0 3.8907250949823263E-4 14-15 0.00904593584583391 0.0 9.726812737455816E-5 0.0 3.8907250949823263E-4 16-17 0.00904593584583391 0.0 9.726812737455816E-5 0.0 3.8907250949823263E-4 18-19 0.00904593584583391 0.0 9.726812737455816E-5 4.863406368727908E-5 3.8907250949823263E-4 20-21 0.00904593584583391 0.0 9.726812737455816E-5 9.726812737455816E-5 3.8907250949823263E-4 22-23 0.009094569909521188 0.0 9.726812737455816E-5 9.726812737455816E-5 3.8907250949823263E-4 24-25 0.009240472100583026 0.0 9.726812737455816E-5 9.726812737455816E-5 3.8907250949823263E-4 26-27 0.009386374291644864 0.0 9.726812737455816E-5 9.726812737455816E-5 4.863406368727908E-4 28-29 0.009435008355332142 1.9453625474911632E-4 9.726812737455816E-5 9.726812737455816E-5 4.863406368727908E-4 30-31 0.00948364241901942 1.9453625474911632E-4 9.726812737455816E-5 9.726812737455816E-5 5.83608764247349E-4 32-33 0.009726812737455817 1.9453625474911632E-4 9.726812737455816E-5 9.726812737455816E-5 5.83608764247349E-4 34-35 0.010213153374328607 1.9453625474911632E-4 9.726812737455816E-5 9.726812737455816E-5 5.83608764247349E-4 36-37 0.010359055565390445 1.9453625474911632E-4 9.726812737455816E-5 9.726812737455816E-5 5.83608764247349E-4 38-39 0.010456323692765002 1.9453625474911632E-4 9.726812737455816E-5 9.726812737455816E-5 5.83608764247349E-4 40-41 0.010748128074888678 1.9453625474911632E-4 9.726812737455816E-5 9.726812737455816E-5 5.83608764247349E-4 42-43 0.010942664329637794 1.9453625474911632E-4 9.726812737455816E-5 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