##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-36C_S49_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1235173
Sequences flagged as poor quality	0
Sequence length	61
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.453922648892096	40.0	40.0	40.0	40.0	40.0
2	39.194856105177166	40.0	40.0	40.0	40.0	40.0
3	39.13477383330108	40.0	40.0	40.0	40.0	40.0
4	39.04193015877128	40.0	40.0	40.0	40.0	40.0
5	39.07237933471667	40.0	40.0	40.0	40.0	40.0
6	39.152151156153835	40.0	40.0	40.0	40.0	40.0
7	39.20314320342171	40.0	40.0	40.0	40.0	40.0
8	39.19590858932312	40.0	40.0	40.0	40.0	40.0
9	39.21947775736678	40.0	40.0	40.0	40.0	40.0
10	39.22804659752116	40.0	40.0	40.0	40.0	40.0
11	39.23085753979402	40.0	40.0	40.0	40.0	40.0
12	39.23172543441283	40.0	40.0	40.0	40.0	40.0
13	39.048282305393656	40.0	40.0	40.0	40.0	40.0
14	39.21855480973111	40.0	40.0	40.0	40.0	40.0
15	39.12509421757114	40.0	40.0	40.0	40.0	40.0
16	39.30608586813345	40.0	40.0	40.0	40.0	40.0
17	39.26978002271746	40.0	40.0	40.0	40.0	40.0
18	39.34768327999398	40.0	40.0	40.0	40.0	40.0
19	39.29989402294254	40.0	40.0	40.0	40.0	40.0
20	39.30803539261302	40.0	40.0	40.0	40.0	40.0
21	39.28865996908935	40.0	40.0	40.0	40.0	40.0
22	39.34605435837733	40.0	40.0	40.0	40.0	40.0
23	39.338288644586626	40.0	40.0	40.0	40.0	40.0
24	39.33541617247138	40.0	40.0	40.0	40.0	40.0
25	39.297121941622756	40.0	40.0	40.0	40.0	40.0
26	39.25696238502623	40.0	40.0	40.0	40.0	40.0
27	39.27534361583357	40.0	40.0	40.0	40.0	40.0
28	39.28974159895011	40.0	40.0	40.0	40.0	40.0
29	39.286571192861246	40.0	40.0	40.0	40.0	40.0
30	39.28688531889865	40.0	40.0	40.0	40.0	40.0
31	39.242033302217585	40.0	40.0	40.0	40.0	40.0
32	39.263419780063195	40.0	40.0	40.0	40.0	40.0
33	39.26719900775033	40.0	40.0	40.0	40.0	40.0
34	39.25610744405844	40.0	40.0	40.0	40.0	40.0
35	39.25680370280114	40.0	40.0	40.0	40.0	40.0
36	39.22369740918884	40.0	40.0	40.0	40.0	40.0
37	39.233409409046345	40.0	40.0	40.0	40.0	40.0
38	39.22749606735251	40.0	40.0	40.0	40.0	40.0
39	37.984144731142926	40.0	40.0	40.0	40.0	40.0
40	39.215627284599	40.0	40.0	40.0	40.0	40.0
41	39.18394589259966	40.0	40.0	40.0	40.0	40.0
42	39.181656334780634	40.0	40.0	40.0	40.0	40.0
43	39.20018977098755	40.0	40.0	40.0	40.0	40.0
44	39.15883200167102	40.0	40.0	40.0	40.0	40.0
45	38.98684961539801	40.0	40.0	40.0	40.0	40.0
46	38.99279210280665	40.0	40.0	40.0	40.0	40.0
47	39.118960663809844	40.0	40.0	40.0	40.0	40.0
48	39.183813117676635	40.0	40.0	40.0	40.0	40.0
49	39.162588560468855	40.0	40.0	40.0	40.0	40.0
50	39.15655863591578	40.0	40.0	40.0	40.0	40.0
51	39.109018736646604	40.0	40.0	40.0	40.0	40.0
52	39.11560242978109	40.0	40.0	40.0	40.0	40.0
53	39.04385215674242	40.0	40.0	40.0	40.0	40.0
54	38.962464367339635	40.0	40.0	40.0	40.0	40.0
55	39.00929505421508	40.0	40.0	40.0	40.0	40.0
56	39.01517601178134	40.0	40.0	40.0	40.0	40.0
57	38.93947487517943	40.0	40.0	40.0	40.0	40.0
58	38.93240865854419	40.0	40.0	40.0	40.0	40.0
59	38.90506997805166	40.0	40.0	40.0	40.0	40.0
60	38.87741393310897	40.0	40.0	40.0	40.0	40.0
61	38.76509282505366	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.05072463451067932
1101	2	0.043811227578345324
1101	3	0.091366760149441
1101	4	0.004937969123290031
1101	5	0.05074739367804426
1101	6	0.06159488017113546
1101	7	0.04515194704169545
1101	8	0.014708824802909248
1101	9	-0.04839661304958298
1101	10	-0.007107397940053772
1101	11	0.11063436396663917
1101	12	-0.01043251412308166
1101	13	8.427698485675705E-4
1101	14	0.0756529180960186
1101	15	0.03972080869608874
1101	16	0.01553008219898544
1101	17	0.05979910383869225
1101	18	-0.10374436092264716
1101	19	-0.021179851585898746
1101	20	0.04845326136427275
1101	21	0.08062187741221294
1101	22	0.014007056447226773
1101	23	-0.01590093877007348
1101	24	-0.05908607090734819
1101	25	0.044476566817721164
1101	26	-0.029934922684894616
1101	27	0.05632540707877354
1101	28	0.04247151270785565
1101	29	0.0618863545828745
1101	30	-0.0045809380237855635
1101	31	0.10191503785510747
1101	32	-0.03610314329147002
1101	33	0.09390832964828633
1101	34	0.027062308044108363
1101	35	0.04035458320680618
1101	36	-0.04022908448664708
1101	37	-0.07162779908716743
1101	38	-0.05967055614868144
1101	39	1.2331664020584654
1101	40	-0.04237982207207125
1101	41	-0.09219714737248808
1101	42	0.08018591103878947
1101	43	-0.056486255603942936
1101	44	0.08638161537264466
1101	45	0.03443208666502784
1101	46	0.047508196213648546
1101	47	-0.07115374451806389
1101	48	0.06893097055971964
1101	49	-0.011925737487089805
1101	50	0.037760899946206905
1101	51	0.001458798228945568
1101	52	0.0028943383631343522
1101	53	-0.1114640877857056
1101	54	0.10373366085130442
1101	55	0.01817061854551838
1101	56	0.07599804821643374
1101	57	0.04229936381586441
1101	58	-0.05844038020838127
1101	59	-0.05348733086301394
1101	60	-0.009224723335670149
1101	61	-0.007433244590671961
1102	1	0.08301816249425542
1102	2	-0.049925792972793204
1102	3	0.0768095853976547
1102	4	-0.15699750073115837
1102	5	-0.084383285809281
1102	6	-0.07379190580741835
1102	7	-0.011844271425829334
1102	8	-0.0598738839194084
1102	9	-0.06238994456818858
1102	10	-0.08266187999534935
1102	11	-0.07759470565368787
1102	12	0.04704064517540729
1102	13	-0.00994335183779782
1102	14	-0.11495625496372242
1102	15	0.11195664136807437
1102	16	-0.024715442646794372
1102	17	0.06116398274402712
1102	18	0.032969475856752695
1102	19	-9.934459331546464E-4
1102	20	-0.013858316975046137
1102	21	-0.06687552510054218
1102	22	0.12221741649154438
1102	23	0.0838195677686926
1102	24	0.05850565905110727
1102	25	-0.01700637155697393
1102	26	-0.005075723746131189
1102	27	-0.08844990548233511
1102	28	0.0601219017878023
1102	29	0.01874697974341899
1102	30	0.09382427509796543
1102	31	0.0414253428186413
1102	32	0.09645880342457502
1102	33	0.09253680526513364
1102	34	-0.02560310364962959
1102	35	0.07602648574320625
1102	36	-0.03073371906843647
1102	37	-0.08156943390027038
1102	38	0.04609375686866457
1102	39	1.3504360819381276
1102	40	-0.05843120695130466
1102	41	0.0018327853931694449
1102	42	0.08921762658167154
1102	43	0.059210490795678083
1102	44	0.04454168704156558
1102	45	0.009218121448995475
1102	46	0.06601994145670176
1102	47	-0.08200427622018225
1102	48	0.06059089590033295
1102	49	-0.04213301249613011
1102	50	0.1254822295018414
1102	51	-0.0016804232533260688
1102	52	0.04734645066659482
1102	53	0.13878006962521283
1102	54	0.10421673596948011
1102	55	0.0033113301483993496
1102	56	-0.11102614515674958
1102	57	-0.07759067908580874
1102	58	0.04118964559396687
1102	59	-0.009766732309941517
1102	60	-0.02234954202275219
1102	61	0.03036930037173846
1103	1	0.05269464460560158
1103	2	0.04366013232904464
1103	3	-0.04898019789516894
1103	4	-0.09272846994419837
1103	5	-0.06586881227561747
1103	6	-0.00894964288109179
1103	7	-0.020156137446690536
1103	8	-0.0336161536644255
1103	9	0.057977247599801274
1103	10	-0.07261413761696645
1103	11	0.024526137690983774
1103	12	0.04257960473596256
1103	13	-0.09280313301567134
1103	14	-0.050176400375150365
1103	15	-0.09082191651393146
1103	16	-0.11321046558185799
1103	17	-0.03249012944536389
1103	18	-0.052694057680220396
1103	19	0.020870275059969856
1103	20	-0.10744770940676318
1103	21	0.01789580034853344
1103	22	-0.029483384068740293
1103	23	-0.02117205911265785
1103	24	0.02143048358544064
1103	25	-0.06396749000684565
1103	26	-0.04282583382750005
1103	27	0.07842305541191052
1103	28	-0.015362138435527584
1103	29	-0.05359121797312838
1103	30	-0.007018353032705704
1103	31	0.027878110686202717
1103	32	0.007797358255501763
1103	33	-0.061953879134748036
1103	34	0.046980104578103976
1103	35	-0.019668610232720596
1103	36	0.021700841744440424
1103	37	0.04689003939647307
1103	38	0.07405269218241983
1103	39	1.2355099722543201
1103	40	-0.025116711690294835
1103	41	-0.030742055494464182
1103	42	-0.0559418556140443
1103	43	-0.03224777247273636
1103	44	0.03014154095982491
1103	45	0.01859092921166905
1103	46	0.05906487892701762
1103	47	-0.07271467957451705
1103	48	0.007638577026085613
1103	49	-0.025000768072715118
1103	50	0.0015508278221290084
1103	51	0.05194220739679167
1103	52	0.11911603908360746
1103	53	-0.01630927253312109
1103	54	0.04964411173354932
1103	55	0.012593992534313259
1103	56	0.03420267963646495
1103	57	0.02827466452409766
1103	58	0.05160490373911841
1103	59	0.077750560000311
1103	60	0.12706064139997153
1103	61	0.07261281077138904
1104	1	0.01702145808622646
1104	2	-0.07021901212599602
1104	3	-0.012225879017051966
1104	4	-0.059682981241408584
1104	5	-0.0227256989149609
1104	6	0.0351669338676075
1104	7	-0.09085728065856813
1104	8	0.0871285863339537
1104	9	-0.06063501958075079
1104	10	0.09127528373679894
1104	11	0.02759078336925569
1104	12	0.022218147980019864
1104	13	0.07443029840879944
1104	14	-0.004483988933856153
1104	15	-0.03247892367979688
1104	16	-0.08357562672533447
1104	17	0.0298975057664137
1104	18	0.02525566378511712
1104	19	0.001011883276369474
1104	20	-0.019810393902581325
1104	21	0.03318348752627287
1104	22	-0.0458216277082002
1104	23	-0.007848807985368467
1104	24	-0.0069760927412119145
1104	25	0.01583220389445472
1104	26	0.019769299352091707
1104	27	0.014991135717281168
1104	28	0.012744689567341538
1104	29	0.05726470199746814
1104	30	-0.052301256519889705
1104	31	-0.033154254423457985
1104	32	-0.10257483534428502
1104	33	-0.08257065769743832
1104	34	-0.0047456892094288605
1104	35	-0.009839112671805594
1104	36	-0.11699221113735803
1104	37	-0.14481012029072104
1104	38	-0.09248454958681407
1104	39	1.090038216869857
1104	40	-0.08259626209916604
1104	41	-0.11771584790006528
1104	42	-0.05041101557524286
1104	43	-0.13961046154187073
1104	44	-2.911458762184793E-4
1104	45	-0.16843843554009652
1104	46	-0.06107801243172872
1104	47	-0.05561229984199656
1104	48	-0.004269036202408927
1104	49	-0.06332517232945634
1104	50	-0.012864531626938458
1104	51	-0.008755652172816042
1104	52	0.0067856814122535525
1104	53	-0.014735005835248671
1104	54	0.062052960607211105
1104	55	0.05969995121478178
1104	56	0.04539162861875212
1104	57	-0.0800753385530939
1104	58	0.0852943156065109
1104	59	-0.016452210724757776
1104	60	0.08349181747310297
1104	61	8.425564936089813E-4
1201	1	0.020678868582884036
1201	2	-0.00979629622229794
1201	3	0.03977599976654744
1201	4	-0.039642796292305604
1201	5	0.03339249015305512
1201	6	0.0019388431131517336
1201	7	0.006669069983793463
1201	8	0.002417090308938441
1201	9	0.005816739424538753
1201	10	-0.04963192524328264
1201	11	0.054035013712493196
1201	12	0.04480413413264017
1201	13	-0.05381762402257095
1201	14	0.036173907908121805
1201	15	-0.0814256329013503
1201	16	-0.0098329802757533
1201	17	-0.06130570528338808
1201	18	0.012972029897134973
1201	19	-0.0032230541640672072
1201	20	0.003950937624814799
1201	21	0.06937907821211553
1201	22	-0.00126698487935073
1201	23	0.0020695669058383714
1201	24	-0.006863969266987624
1201	25	-0.013133894103283694
1201	26	0.04041380641398007
1201	27	-0.032530226601139134
1201	28	-0.009092592736543281
1201	29	0.07885666542387781
1201	30	0.0758631586371763
1201	31	-0.0010476585584697773
1201	32	-0.05846790597049534
1201	33	-0.007029353009436079
1201	34	0.11658101505112484
1201	35	0.0774691734220383
1201	36	-0.015236906441813858
1201	37	-0.006328784621786099
1201	38	0.055012189792314814
1201	39	1.3619706805568796
1201	40	0.07532457846788532
1201	41	-0.017847131220868562
1201	42	-6.224697815824243E-4
1201	43	-0.015381294167923443
1201	44	-0.03474400998711502
1201	45	-0.0383767663285397
1201	46	0.10571801246592827
1201	47	-0.004879159303811775
1201	48	-0.007850483695648336
1201	49	-0.03228960278318027
1201	50	0.01438147260851963
1201	51	-0.021940176063637296
1201	52	-0.01244570566828429
1201	53	0.02029330404125318
1201	54	0.03295210632259682
1201	55	0.012892433010648574
1201	56	-0.013924290716339272
1201	57	-0.03152821554128593
1201	58	0.016864136597874335
1201	59	0.007704284508761816
1201	60	-0.027650901531551142
1201	61	0.016439426037429428
1202	1	0.007189437050371339
1202	2	0.007813557425421891
1202	3	0.047792488609850636
1202	4	0.04741578192825813
1202	5	-0.02402996797451351
1202	6	0.18529034463330163
1202	7	-0.02563963894039034
1202	8	0.06429471492200634
1202	9	0.0962516362440553
1202	10	0.013106580120549438
1202	11	0.10538250034444019
1202	12	0.024073236409563492
1202	13	0.024010953129874224
1202	14	0.012266260557993292
1202	15	0.12146880712242591
1202	16	-0.041598687835914916
1202	17	0.060486306540497026
1202	18	0.017054633564406174
1202	19	-0.06249989389101529
1202	20	-0.007422194003318339
1202	21	0.05420468150579438
1202	22	-0.053992289560611084
1202	23	0.03238718565193466
1202	24	0.10546959762081087
1202	25	-0.023741968750769615
1202	26	0.05616212362081541
1202	27	0.039751304146534494
1202	28	-0.009533126549044368
1202	29	0.002868671247924226
1202	30	0.13470610833302032
1202	31	0.08235811754506983
1202	32	0.09576569040547867
1202	33	-0.05927707202041432
1202	34	-0.0931726182029422
1202	35	-0.022017875548257848
1202	36	-0.17941701015635658
1202	37	-0.12881174971075637
1202	38	-0.30648311075447054
1202	39	1.1296746970293654
1202	40	-0.18680479435403896
1202	41	-0.18639705438297938
1202	42	-0.09195913216045426
1202	43	0.0038041577906327007
1202	44	-0.23801944532089436
1202	45	0.09593391835401377
1202	46	-0.019706870130789866
1202	47	-0.03993174994415227
1202	48	-0.026247880968732318
1202	49	-0.09038129412127205
1202	50	-0.07699990752974628
1202	51	-0.07223130145376899
1202	52	-0.08103073865976995
1202	53	0.05399541834802335
1202	54	0.12700147575107934
1202	55	0.05451010178952487
1202	56	-0.07700546188212343
1202	57	0.10250138608225257
1202	58	0.02344965714070213
1202	59	-0.06601076983992726
1202	60	0.020828260248102026
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2403	4	0.011121954052278227
2403	5	0.04055276166906907
2403	6	0.12527150951304833
2403	7	0.06310275456873882
2403	8	-0.03448100226142259
2403	9	0.006748593647706969
2403	10	0.07106079628977824
2403	11	0.05912779156437864
2403	12	0.09564669337855491
2403	13	0.014122514995996482
2403	14	0.02414687487268452
2403	15	0.11154414760343911
2403	16	-0.013853106844898377
2403	17	0.05461844215336242
2403	18	-0.005511289356491034
2403	19	-7.42432285683492E-4
2403	20	-0.0277061696625438
2403	21	-0.02990137470979448
2403	22	0.07365187155210862
2403	23	0.08353178142127149
2403	24	-0.004027649553918877
2403	25	-0.011788911665867374
2403	26	0.07108533428439046
2403	27	0.0068985078556025314
2403	28	-0.010728432257536724
2403	29	0.010294055194606244
2403	30	-0.015141761849534419
2403	31	0.04867222939670768
2403	32	-0.011956708899070634
2403	33	0.07271365530499452
2403	34	0.0403394072969121
2403	35	0.0014535820002805622
2403	36	0.08409356648810018
2403	37	0.026541771007877912
2403	38	0.061779711615855604
2403	39	1.2841951258905695
2403	40	-0.03047329623755246
2403	41	0.1287652174222913
2403	42	-0.0033254028066451724
2403	43	0.07733356955534276
2403	44	0.08083740776516635
2403	45	0.0568872652100012
2403	46	0.10938614638616428
2403	47	0.12136207793182763
2403	48	0.07817837229119107
2403	49	0.018137975655598382
2403	50	0.13848111246446138
2403	51	0.011693647836295895
2403	52	-0.02738686731812834
2403	53	0.04638892747581025
2403	54	0.030471484444213104
2403	55	-0.01065217001574581
2403	56	0.09435791105325109
2403	57	0.01249124409442004
2403	58	0.02431897038324138
2403	59	0.10010050225511691
2403	60	0.09276393106815561
2403	61	0.06021434341178633
2404	1	0.01776076635474766
2404	2	-0.14754676018019808
2404	3	-0.06335341950357787
2404	4	-0.03368211167536117
2404	5	0.05613884566719918
2404	6	-0.07781942599021363
2404	7	-0.007018959991988538
2404	8	0.016227206596155952
2404	9	0.02293515536661772
2404	10	0.03270451949560993
2404	11	0.06725863693498724
2404	12	0.021607734960667813
2404	13	-0.06868103397703607
2404	14	0.0763690305599809
2404	15	0.10035130942303994
2404	16	-0.021562841570137437
2404	17	0.04175668620791129
2404	18	0.027100528133772173
2404	19	-0.00852064254048912
2404	20	-0.0396913279380513
2404	21	0.004426766681362437
2404	22	0.06449444769579316
2404	23	0.0637542742188657
2404	24	0.011587820703859109
2404	25	-0.031195486647405346
2404	26	0.0032540846032063087
2404	27	0.12364928575979661
2404	28	0.14933713791352687
2404	29	0.15037530543025213
2404	30	-0.010346534178104605
2404	31	-0.10902941024568946
2404	32	0.055055606716010175
2404	33	-0.07091640162672519
2404	34	0.07067656195291505
2404	35	0.07379700868212069
2404	36	0.04583736931405724
2404	37	0.018658213784512157
2404	38	-0.043090146065928536
2404	39	1.311175593453683
2404	40	0.12060423691347211
2404	41	-0.003457703416664515
2404	42	0.11506268877202785
2404	43	-0.02551445801672969
2404	44	0.017942218018646372
2404	45	0.02359041519525107
2404	46	0.04045928823197187
2404	47	0.11858840314124564
2404	48	0.05548358574633028
2404	49	-0.06396501597652104
2404	50	0.06639024127829884
2404	51	0.04960719013365633
2404	52	0.058814398619759345
2404	53	-0.08162482751153277
2404	54	0.012762381800783373
2404	55	-0.019778821468094065
2404	56	0.0012503196398085947
2404	57	0.0631700666923436
2404	58	0.08775558810238238
2404	59	-0.09482188475193709
2404	60	-0.02853423190249771
2404	61	0.06484545159536026
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	5.0
17	48.0
18	109.0
19	151.0
20	237.0
21	308.0
22	459.0
23	619.0
24	972.0
25	1301.0
26	1687.0
27	2243.0
28	3023.0
29	3598.0
30	4603.0
31	5663.0
32	7769.0
33	9359.0
34	12135.0
35	15318.0
36	22695.0
37	32407.0
38	81448.0
39	540799.0
40	488215.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	31.02197020174502	14.0289659829028	22.603797200877935	32.34526661447425
2	31.73010187264583	17.62512583243776	24.59788285658058	26.04688943833583
3	27.011725501975032	20.557882268932804	19.73977692157604	32.690615307516126
4	27.660277273124716	20.523295541025867	20.550012467899208	31.266414717950212
5	27.734900293319235	21.209093786862248	19.368137094965647	31.687868824852877
6	29.092523881270072	24.182442459477336	21.95821961781872	24.76681404143387
7	28.012594187211022	23.083406130153428	21.913529521775494	26.99047016086006
8	25.66725470844975	26.4484408256981	22.241175932440232	25.643128533411918
9	25.023782093682424	25.89515800620642	23.306613729412803	25.774446170698358
10	26.86004308708173	23.35713296841819	22.850159451348112	26.932664493151968
11	25.535532269568716	24.56465612509341	22.968523437607523	26.93128816773035
12	27.17829810075188	23.529983249310014	22.786767521634623	26.50495112830348
13	25.642480850860565	21.826578139256604	22.88513430912107	29.645806700761757
14	25.926651570265864	24.264779103817848	22.82174237940758	26.986826946508707
15	25.729594154017292	22.956298429450772	22.825871355672444	28.48823606085949
16	26.26514666366574	24.09403379121791	22.219235686013214	27.421583859103137
17	26.190258368665763	22.51239300081851	22.42196032458611	28.87538830592962
18	27.657340307794943	23.241197791726343	22.516683897721208	26.58477800275751
19	26.24968324275223	22.1124490253592	23.85876310443962	27.779104627448948
20	26.28781555296303	22.942373254596724	22.34326689459695	28.426544297843297
21	25.878075378914534	22.802230942548128	24.1921576977476	27.127535980789734
22	27.839905826957036	22.218911844737537	22.258501440688875	27.682680887616552
23	26.313884775654913	23.870097549088264	22.401315443261794	27.41470223199503
24	26.178438162103607	24.755722477741983	22.47426069060771	26.591578669546696
25	26.38521081662245	22.05990577838084	22.26773091704563	29.28715248795108
26	26.45677973854675	22.990787525310218	23.809620190855856	26.742812545287176
27	26.27931471947654	23.155703694947995	22.280036885521298	28.28494470005416
28	26.49272612014673	22.841739578180547	21.965101244926824	28.7004330567459
29	26.531101311314288	24.48288620298533	22.08265562799705	26.903356857703336
30	27.90872209803809	23.773673809255868	22.150824216526754	26.166779876179287
31	26.543731121065633	22.65820253519143	23.578316559704593	27.21974978403835
32	26.668248091562884	23.403928032753306	21.943241958818724	27.984581916865086
33	26.387963467465692	25.10118015856888	22.09722848540245	26.413627888562978
34	26.809766729033097	23.352356309601973	21.61931972282425	28.218557238540676
35	26.871863293643887	25.170644112201284	21.6156765084729	26.341816085681923
36	26.840693570860115	24.319427319088096	23.17221960000745	25.667659510044345
37	27.02010163758437	24.9525370130338	21.388420893267583	26.638940456114245
38	28.575673205291892	24.37367073276375	21.269328264137897	25.781327797806462
39	27.212313955392748	25.976723743185747	21.190284402167194	25.620677899254318
40	28.681812183394552	24.33375729553674	20.86630779655967	26.118122724509035
41	27.156681695600536	24.539963227823147	20.906302194105603	27.397052882470714
42	28.596237126297286	25.24310359763369	20.856106796375894	25.304552479693125
43	27.2465476496005	26.110269573573902	20.56351620380303	26.079666573022564
44	27.269702300811304	25.21994894642289	22.031812547715987	25.478536205049817
45	27.245576125773475	25.540956610936284	20.423292931435515	26.790174331854725
46	27.320464420773444	25.316291725936367	21.70829511331611	25.654948739974078
47	29.077465261951158	25.51270145963359	20.098642052570774	25.311191225844475
48	28.751114216389123	26.236891512363048	20.043103273792415	24.968890997455418
49	29.157292136405182	25.619569080606524	19.7005601644466	25.52257861854169
50	28.901538488940414	26.37565749899002	19.581953297230427	25.140850714839136
51	28.82754075744855	26.440830555719725	19.659675203392563	25.071953483439163
52	28.92825539418365	26.379543594298127	19.19455817120355	25.497642840314676
53	29.013344689367397	26.58534472498994	19.28741965700351	25.113890928639147
54	28.743665867048584	27.064791733627597	19.202654203095438	24.98888819622838
55	28.89530454438366	26.657642289784505	18.906177515214466	25.540875650617362
56	28.67460671501077	27.156195933687023	18.883832467192853	25.285364884109352
57	28.623925555367546	27.343942913259923	18.867235601814482	25.164895929558046
58	28.538917220502714	27.285246682043727	18.669611463333478	25.506224634120077
59	28.5547044826919	27.528856281670667	18.583793525279454	25.33264571035798
60	28.210380246329862	27.98668688515698	18.605733771706472	25.19719909680668
61	28.733383906545885	27.376003199551803	18.306261552025504	25.584351341876804
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	2.0
2	0.5
3	1.0
4	1.0
5	1.0
6	1.5
7	2.5
8	3.0
9	3.5
10	4.0
11	5.5
12	18.0
13	29.0
14	34.0
15	39.0
16	55.5
17	109.5
18	147.0
19	183.5
20	311.5
21	403.0
22	540.0
23	677.0
24	917.0
25	1425.5
26	1694.0
27	2090.0
28	2486.0
29	3130.5
30	4555.5
31	5336.0
32	6416.0
33	7496.0
34	8686.0
35	11550.5
36	13225.0
37	15510.5
38	17796.0
39	20662.0
40	26571.0
41	29614.0
42	33641.5
43	41758.0
44	45847.0
45	50473.0
46	55099.0
47	59508.5
48	67683.5
49	71449.0
50	74428.5
51	77408.0
52	79216.5
53	82133.0
54	83241.0
55	83499.0
56	83757.0
57	82129.5
58	78117.0
59	75732.0
60	72719.5
61	74413.0
62	79119.0
63	66073.5
64	53028.0
65	48294.5
66	38956.0
67	34351.0
68	30192.5
69	26034.0
70	22726.0
71	16413.0
72	13408.0
73	11397.5
74	9387.0
75	7608.5
76	4676.5
77	3523.0
78	2790.0
79	2057.0
80	1613.0
81	905.5
82	642.0
83	485.0
84	244.0
85	160.0
86	133.5
87	107.0
88	76.5
89	34.0
90	22.0
91	20.0
92	18.0
93	10.5
94	3.0
95	3.0
96	4.5
97	6.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.032060286291879764
3	1.6192063783777657E-4
4	8.096031891888828E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.110252571906931
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1235173.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.57638421949775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.29237928003522	72.95581430142349
2	9.730606581384743	16.264978286691697
3	1.9625912871996356	4.920788504545063
4	0.594012069361583	1.9858152375993046
5	0.2241564971521048	0.9367094765640533
6	0.08599493886079157	0.43122876306970404
7	0.03965254196729103	0.23198112579166522
8	0.020256826970588563	0.13543938831694355
9	0.012753588753966467	0.09593089504910703
>10	0.036334563991207124	0.4447289598609561
>50	9.467752826587305E-4	0.04764012377608096
>100	2.100326934723813E-4	0.02492635778539131
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	1.0501634673619065E-4	1.5240185795265306
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGAGCGATCTGGGGGGGGGGGGGGG	17364	1.4057949777075762	TruSeq Adapter, Index 3 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.544480004015632	0.0	0.0	0.0	0.0
2	1.5477184167723874	0.0	0.0	0.0	0.0
3	1.5498233850642784	0.0	0.0	0.0	0.0
4	1.5514425914426562	0.0	0.0	0.0	0.0
5	1.5546000438804928	0.0	0.0	0.0	2.4288095675666487E-4
6	1.5603482265237338	0.0	0.0	0.0	4.8576191351332973E-4
7	1.5665821710804884	0.0	0.0	0.0	4.8576191351332973E-4
8	1.6264118467615467	0.0	0.0	0.0	4.8576191351332973E-4
9	1.6341030770588412	0.0	0.0	0.0	4.8576191351332973E-4
10	1.6379891723669477	0.0	0.0	0.0	5.66722232432218E-4
11	1.643575434372351	0.0	0.0	0.0	5.66722232432218E-4
12	1.6549908393399144	0.0	0.0	0.0	5.66722232432218E-4
13	1.6785502921453108	0.0	0.0	0.0	5.66722232432218E-4
14	1.711096340350704	0.0	0.0	0.0	5.66722232432218E-4
15	1.7475284838642036	0.0	8.096031891888828E-5	0.0	5.66722232432218E-4
16	1.790680333847971	0.0	8.096031891888828E-5	0.0	5.66722232432218E-4
17	1.8369896362695752	8.096031891888828E-5	8.096031891888828E-5	0.0	5.66722232432218E-4
18	1.8888042403776637	8.096031891888828E-5	8.096031891888828E-5	0.0	5.66722232432218E-4
19	1.9629638925073654	8.096031891888828E-5	8.096031891888828E-5	0.0	6.476825513511063E-4
20	2.0558253783073304	8.096031891888828E-5	8.096031891888828E-5	0.0	6.476825513511063E-4
21	2.174108404247826	8.096031891888828E-5	8.096031891888828E-5	0.0	6.476825513511063E-4
22	2.3160318433126372	8.096031891888828E-5	8.096031891888828E-5	0.0	8.096031891888829E-4
23	2.487991560696356	8.096031891888828E-5	8.096031891888828E-5	0.0	8.096031891888829E-4
24	2.7036698502962744	8.096031891888828E-5	8.096031891888828E-5	0.0	8.096031891888829E-4
25	2.9552135611772603	1.6192063783777657E-4	8.096031891888828E-5	0.0	8.096031891888829E-4
26	3.267558471566331	1.6192063783777657E-4	8.096031891888828E-5	0.0	8.096031891888829E-4
27	3.6050010808202577	1.6192063783777657E-4	8.096031891888828E-5	0.0	8.096031891888829E-4
28	3.9904531591930845	1.6192063783777657E-4	8.096031891888828E-5	0.0	8.096031891888829E-4
29	4.407965523857792	1.6192063783777657E-4	8.096031891888828E-5	0.0	8.096031891888829E-4
30	4.890812865890041	1.6192063783777657E-4	8.096031891888828E-5	0.0	8.096031891888829E-4
31	5.401996319543902	1.6192063783777657E-4	8.096031891888828E-5	0.0	8.096031891888829E-4
32	5.975762099722063	1.6192063783777657E-4	8.096031891888828E-5	0.0	8.096031891888829E-4
33	6.5895222774461555	1.6192063783777657E-4	8.096031891888828E-5	0.0	8.096031891888829E-4
34	7.286752543975621	1.6192063783777657E-4	8.096031891888828E-5	0.0	8.096031891888829E-4
35	8.008028025223997	1.6192063783777657E-4	8.096031891888828E-5	0.0	8.096031891888829E-4
36	8.810749587304775	2.4288095675666487E-4	8.096031891888828E-5	0.0	8.096031891888829E-4
37	9.658485086704454	2.4288095675666487E-4	8.096031891888828E-5	0.0	8.096031891888829E-4
38	10.57204132538519	2.4288095675666487E-4	8.096031891888828E-5	0.0	8.096031891888829E-4
39	11.498713135730783	2.4288095675666487E-4	8.096031891888828E-5	0.0	8.096031891888829E-4
40	12.564150932703354	2.4288095675666487E-4	8.096031891888828E-5	0.0	8.096031891888829E-4
41	13.643513904529973	2.4288095675666487E-4	8.096031891888828E-5	0.0	8.096031891888829E-4
42	14.807885211221423	2.4288095675666487E-4	8.096031891888828E-5	0.0	8.905635081077712E-4
43	16.019294463204748	2.4288095675666487E-4	1.6192063783777657E-4	0.0	9.715238270266595E-4
44	17.27814646207454	2.4288095675666487E-4	1.6192063783777657E-4	0.0	9.715238270266595E-4
45	18.57173043776054	2.4288095675666487E-4	1.6192063783777657E-4	0.0	9.715238270266595E-4
46	19.889683469441124	2.4288095675666487E-4	1.6192063783777657E-4	0.0	0.001133444464864436
47	21.25240755748385	2.4288095675666487E-4	1.6192063783777657E-4	0.0	0.001133444464864436
48	22.68848169446709	2.4288095675666487E-4	1.6192063783777657E-4	0.0	0.001133444464864436
49	24.086504481558453	2.4288095675666487E-4	1.6192063783777657E-4	0.0	0.001133444464864436
50	25.555448508022764	2.4288095675666487E-4	1.6192063783777657E-4	0.0	0.001133444464864436
>>END_MODULE