##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-36C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 767678 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.55976854879259 40.0 40.0 40.0 40.0 40.0 2 39.34983938578414 40.0 40.0 40.0 40.0 40.0 3 39.29838552101272 40.0 40.0 40.0 40.0 40.0 4 39.207891850489396 40.0 40.0 40.0 40.0 40.0 5 39.23919143182428 40.0 40.0 40.0 40.0 40.0 6 39.330031601791376 40.0 40.0 40.0 40.0 40.0 7 39.372512954650254 40.0 40.0 40.0 40.0 40.0 8 39.37269011226061 40.0 40.0 40.0 40.0 40.0 9 39.39370413115916 40.0 40.0 40.0 40.0 40.0 10-11 39.397093573086636 40.0 40.0 40.0 40.0 40.0 12-13 39.31332668123875 40.0 40.0 40.0 40.0 40.0 14-15 39.341132610287126 40.0 40.0 40.0 40.0 40.0 16-17 39.451616432931516 40.0 40.0 40.0 40.0 40.0 18-19 39.49057808091413 40.0 40.0 40.0 40.0 40.0 20-21 39.4698636667978 40.0 40.0 40.0 40.0 40.0 22-23 39.511641599733224 40.0 40.0 40.0 40.0 40.0 24-25 39.488334952936 40.0 40.0 40.0 40.0 40.0 26-27 39.46025547169516 40.0 40.0 40.0 40.0 40.0 28-29 39.484033670367005 40.0 40.0 40.0 40.0 40.0 30-31 39.462059948756455 40.0 40.0 40.0 40.0 40.0 32-33 39.46255250731049 40.0 40.0 40.0 40.0 40.0 34-35 39.45758457128471 40.0 40.0 40.0 40.0 40.0 36-37 39.44059068944306 40.0 40.0 40.0 40.0 40.0 38-39 39.38492205069617 40.0 40.0 40.0 40.0 40.0 40-41 39.42895138784033 40.0 40.0 40.0 40.0 40.0 42-43 39.40027830271839 40.0 40.0 40.0 40.0 40.0 44-45 39.30694608686032 40.0 40.0 40.0 40.0 40.0 46-47 39.2821024610995 40.0 40.0 40.0 40.0 40.0 48-49 39.374517825829955 40.0 40.0 40.0 40.0 40.0 50-51 39.3329403713452 40.0 40.0 40.0 40.0 40.0 52-53 39.28033833904311 40.0 40.0 40.0 40.0 40.0 54-55 39.18979052562545 40.0 40.0 40.0 40.0 40.0 56-57 39.208006486079924 40.0 40.0 40.0 40.0 40.0 58-59 39.16655504757469 40.0 40.0 40.0 40.0 40.0 60-61 39.078641062075945 40.0 40.0 40.0 40.0 40.0 62-63 39.390963604998944 40.0 40.0 40.0 40.0 40.0 64-65 39.389679264424345 40.0 40.0 40.0 40.0 40.0 66-67 39.392611179376985 40.0 40.0 40.0 40.0 40.0 68-69 39.386004146375825 40.0 40.0 40.0 40.0 40.0 70-71 39.389233325583064 40.0 40.0 40.0 40.0 40.0 72-73 39.3973186038439 40.0 40.0 40.0 40.0 40.0 74-75 39.395951424506976 40.0 40.0 40.0 40.0 40.0 76-77 39.39601780938808 40.0 40.0 40.0 40.0 40.0 78-79 39.391640832727475 40.0 40.0 40.0 40.0 40.0 80-81 39.393447645798425 40.0 40.0 40.0 40.0 40.0 82-83 39.36616690988323 40.0 40.0 40.0 40.0 40.0 84-85 39.37185539023581 40.0 40.0 40.0 40.0 40.0 86-87 39.3477063285592 40.0 40.0 40.0 40.0 40.0 88-89 39.41314949300711 40.0 40.0 40.0 40.0 40.0 90-91 39.51668035630269 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05203241880040821 1101 2 0.02893912517186692 1101 3 0.04033802470604542 1101 4 -0.017158537096953808 1101 5 0.046144220130287295 1101 6 0.12833679569682488 1101 7 -0.030575756624941164 1101 8 -0.041466046042614835 1101 9 0.08316765738125298 1101 10-11 -0.05159739617698733 1101 12-13 0.05573168972399145 1101 14-15 7.121705400692235E-4 1101 16-17 0.015246090564644987 1101 18-19 0.03940831126602973 1101 20-21 0.02775373120377367 1101 22-23 -0.018430191475729885 1101 24-25 0.07353411091366269 1101 26-27 0.043011574228032146 1101 28-29 -6.896366919306729E-4 1101 30-31 0.0028579150410479315 1101 32-33 0.05197298541299489 1101 34-35 -0.003828357497290824 1101 36-37 -0.11399014126801177 1101 38-39 -0.02748841332118701 1101 40-41 -0.028858701785111407 1101 42-43 -0.0025394637690965283 1101 44-45 0.06878276700231112 1101 46-47 0.004856937055876642 1101 48-49 0.009917338135622344 1101 50-51 0.06295035464788157 1101 52-53 0.1045539894592693 1101 54-55 -0.033696007166547304 1101 56-57 0.05800917297649022 1101 58-59 0.14763316067315913 1101 60-61 0.04226236308164033 1101 62-63 -0.02812831328328258 1101 64-65 0.1582732806356546 1101 66-67 -0.014368359301229816 1101 68-69 0.010337103794590519 1101 70-71 -0.06407086763427117 1101 72-73 0.08018062103985102 1101 74-75 0.1713752541786846 1101 76-77 0.0018885466111342453 1101 78-79 0.020099239928832446 1101 80-81 0.020444515685056786 1101 82-83 0.1892759790081655 1101 84-85 0.24575698713816507 1101 86-87 0.27506400328149283 1101 88-89 -0.3264743190635926 1101 90-91 0.5176196156346009 1102 1 0.026983395013218114 1102 2 -0.008618576739152672 1102 3 0.11090304669062334 1102 4 -0.10869721412458944 1102 5 0.023039096078797172 1102 6 -0.028178800566408313 1102 7 0.08864116196106409 1102 8 -0.030353175623751838 1102 9 -0.07335362279933122 1102 10-11 -0.05490543607809428 1102 12-13 -0.015150413650225403 1102 14-15 0.017234912299400662 1102 16-17 -0.028651615162715416 1102 18-19 -0.049570166986896425 1102 20-21 -0.006250710371645596 1102 22-23 0.01062802401781937 1102 24-25 -0.031673981360683 1102 26-27 0.027022714706049555 1102 28-29 0.05990498226230301 1102 30-31 0.002200204604150713 1102 32-33 0.0768727125733264 1102 34-35 0.005539290999699631 1102 36-37 -0.043422830276519164 1102 38-39 -0.01657710272980495 1102 40-41 -0.022025618981174944 1102 42-43 0.0015899404427131003 1102 44-45 0.06230037948203204 1102 46-47 -0.04108013402341015 1102 48-49 0.05142839650439157 1102 50-51 0.08414673394135264 1102 52-53 0.10612234163392031 1102 54-55 -0.09028559804661285 1102 56-57 -0.06880354888173912 1102 58-59 0.017269377461182955 1102 60-61 -0.00933505300987747 1102 62-63 -0.06336084813096221 1102 64-65 0.017944768625724805 1102 66-67 0.013276078909022715 1102 68-69 -0.037408812011349823 1102 70-71 0.07907750033503191 1102 72-73 0.1507320774937 1102 74-75 -0.06559183020657855 1102 76-77 -0.03952712219309973 1102 78-79 -0.01148841971723158 1102 80-81 -0.25948783079336124 1102 82-83 -0.1370257695527215 1102 84-85 -0.08346960001328796 1102 86-87 -0.16453073358750459 1102 88-89 0.37064815711514143 1102 90-91 -0.6667775474859639 1103 1 0.03753087783704956 1103 2 0.01842281363261833 1103 3 0.10655574101470933 1103 4 -0.09573190160215006 1103 5 0.0016616513178249193 1103 6 0.059638689199708494 1103 7 -0.08115112811466219 1103 8 0.042746673561133264 1103 9 0.03196640050120436 1103 10-11 -0.02254960815096041 1103 12-13 0.0987022343919719 1103 14-15 0.04258063002775714 1103 16-17 -0.005419703002544907 1103 18-19 -0.05260083272793281 1103 20-21 0.012649855384538 1103 22-23 0.03730953280738447 1103 24-25 -0.009245618542585987 1103 26-27 0.06235813870399909 1103 28-29 0.1092670103665796 1103 30-31 0.09898520927224297 1103 32-33 0.0770263267099196 1103 34-35 -9.344553177754733E-4 1103 36-37 0.06562156997090796 1103 38-39 0.04740681710577377 1103 40-41 0.08859609481389441 1103 42-43 0.024950163692096794 1103 44-45 -0.03424974378520318 1103 46-47 0.18451421786086541 1103 48-49 0.04383909684052867 1103 50-51 0.1415208904745242 1103 52-53 0.2540352584625154 1103 54-55 -0.06607028691502848 1103 56-57 0.1267507630784408 1103 58-59 0.10433894847459868 1103 60-61 0.21799017826216271 1103 62-63 -0.09306417673813883 1103 64-65 -0.024583121804262476 1103 66-67 0.00515183730460933 1103 68-69 -3.551245703121708E-4 1103 70-71 0.04221446577074062 1103 72-73 -0.049807586253798775 1103 74-75 0.09647755198220409 1103 76-77 0.07312123335164955 1103 78-79 -0.20599173128080395 1103 80-81 0.059074657593328084 1103 82-83 0.08386322101643628 1103 84-85 -0.1032892368050824 1103 86-87 -0.07043787065904183 1103 88-89 0.02513882685953206 1103 90-91 -0.6458419228269392 1104 1 -0.024542324254440473 1104 2 0.046898230390560514 1104 3 -0.059793456263939504 1104 4 -0.20434770332347085 1104 5 -0.18809138924523694 1104 6 -0.03341952516197466 1104 7 -0.1360415853023298 1104 8 -0.15046360863740915 1104 9 -0.07507792774944733 1104 10-11 0.031973378220072846 1104 12-13 -0.042681170213235475 1104 14-15 -0.11808427785850029 1104 16-17 -0.0810297997989693 1104 18-19 -0.03134387617996026 1104 20-21 -0.009597605654022345 1104 22-23 -0.0056075664113208745 1104 24-25 -0.06011532593105784 1104 26-27 0.007420854781607034 1104 28-29 -0.004829106665340532 1104 30-31 -0.0414886455363046 1104 32-33 -0.03505824641039368 1104 34-35 -0.06953911344073305 1104 36-37 -0.09068519122036633 1104 38-39 -0.09405815200926781 1104 40-41 -0.12448388285503142 1104 42-43 -0.07278735986535168 1104 44-45 -0.14612398556656814 1104 46-47 -0.17357483837999865 1104 48-49 -0.09292557048846106 1104 50-51 -0.1681328010381904 1104 52-53 -0.0773136818043696 1104 54-55 -0.0826856117415602 1104 56-57 -0.0012522336977340842 1104 58-59 0.014535311224157965 1104 60-61 -0.07493513983283151 1104 62-63 -0.11103550666192064 1104 64-65 -0.025622399209467517 1104 66-67 -0.05806957399597934 1104 68-69 -0.052570704864351114 1104 70-71 0.15583250236393553 1104 72-73 0.045598720867552345 1104 74-75 -0.11927226891564402 1104 76-77 0.037838167000082024 1104 78-79 0.15954399238074757 1104 80-81 0.24444557952396906 1104 82-83 -0.018050737659152105 1104 84-85 -0.1077975837216485 1104 86-87 0.11035956560149174 1104 88-89 -0.21811703535254168 1104 90-91 0.5176196156346009 1201 1 0.17429204820422228 1201 2 0.10771837055754929 1201 3 0.1126434868211561 1201 4 0.12124648454975784 1201 5 -0.00441825955193309 1201 6 0.0834388763763485 1201 7 0.15154962548872675 1201 8 -0.013087435757391574 1201 9 -0.029191548296701342 1201 10-11 -0.01641015176237204 1201 12-13 -0.035841648892535716 1201 14-15 0.04873536454288541 1201 16-17 -2.4355207973059123E-4 1201 18-19 0.09144134430324868 1201 20-21 0.01865415911526469 1201 22-23 0.03437299853678866 1201 24-25 0.033904511771993384 1201 26-27 0.016120259840747053 1201 28-29 -0.01137416951220871 1201 30-31 0.01642495567101321 1201 32-33 -0.042529166629428516 1201 34-35 0.04954002565101234 1201 36-37 -0.034048387965974314 1201 38-39 0.05520794931759099 1201 40-41 -0.011412288500579848 1201 42-43 0.061587515418160876 1201 44-45 0.061504066687732006 1201 46-47 -0.05942891768874148 1201 48-49 0.03813515634043796 1201 50-51 -0.06972898818808204 1201 52-53 0.004010973390379036 1201 54-55 -0.03648431739822655 1201 56-57 -0.04730252322742956 1201 58-59 -0.0149563882002397 1201 60-61 -0.04923281339999619 1201 62-63 -0.03760764087445523 1201 64-65 -0.01824686830614297 1201 66-67 0.1136367026974483 1201 68-69 0.06863866495594806 1201 70-71 -0.07716018682013015 1201 72-73 -0.09601934324771122 1201 74-75 -0.02546090606031015 1201 76-77 0.049038055286693805 1201 78-79 0.015623364206817314 1201 80-81 0.06836868581288513 1201 82-83 0.14166977873048126 1201 84-85 0.1188383726749791 1201 86-87 -0.06419043897628285 1201 88-89 0.10367150575387996 1201 90-91 -0.698573321488567 1202 1 0.047301260805888035 1202 2 0.12475974139173474 1202 3 0.06873943858854403 1202 4 0.11784783292870316 1202 5 0.03587181287941377 1202 6 0.13763194999690853 1202 7 0.03178600095966999 1202 8 -0.03964092971756372 1202 9 -0.02537171311434605 1202 10-11 0.11592272601177456 1202 12-13 0.01801502999226301 1202 14-15 0.03474323649520272 1202 16-17 -0.05914970130056929 1202 18-19 0.05511347340784312 1202 20-21 0.012703955395323874 1202 22-23 0.003068569779806296 1202 24-25 0.05860448256914452 1202 26-27 -0.0015209426383222535 1202 28-29 -0.0495165212742279 1202 30-31 0.01844292971892969 1202 32-33 0.05340503448141476 1202 34-35 0.02588865172003807 1202 36-37 -0.17597621185574752 1202 38-39 -0.07947885609751637 1202 40-41 -0.15634008461282178 1202 42-43 -0.05586663535398628 1202 44-45 -0.11337654057599167 1202 46-47 -0.06790769443593803 1202 48-49 0.08791180211473204 1202 50-51 -0.05733263255781651 1202 52-53 0.0024620529806114178 1202 54-55 0.01716341868101523 1202 56-57 -0.07020847937399566 1202 58-59 -0.2019921458805669 1202 60-61 0.05766415923812218 1202 62-63 -0.23211430654374254 1202 64-65 -0.31362298193528915 1202 66-67 -0.1548868592906203 1202 68-69 0.046366236073581035 1202 70-71 -0.05602830847463025 1202 72-73 0.0925151431157758 1202 74-75 -0.08460148650393506 1202 76-77 -0.13595936273973308 1202 78-79 -0.3855730515160616 1202 80-81 -0.1271641267364032 1202 82-83 -0.3165057048681703 1202 84-85 -0.0680644355019524 1202 86-87 -0.18445277406360105 1202 88-89 -0.15927560857572587 1202 90-91 -0.3592572612422771 1203 1 0.06903480053900779 1203 2 0.13413709652877515 1203 3 -0.03404368177503159 1203 4 -0.09003712864936375 1203 5 -0.056139860160293154 1203 6 -0.11846953673687466 1203 7 0.0661728138802431 1203 8 0.1070572860628829 1203 9 0.13732877452003578 1203 10-11 0.046870281733262686 1203 12-13 0.0034755619913440228 1203 14-15 0.0424460922990022 1203 16-17 0.08127033351201618 1203 18-19 -6.085104528565921E-4 1203 20-21 -0.022462913666259965 1203 22-23 -0.04727642831870327 1203 24-25 0.020700665000603635 1203 26-27 0.07201960469180335 1203 28-29 0.004442392723987609 1203 30-31 0.10443339547877883 1203 32-33 -0.04967423716448849 1203 34-35 0.054527907309442014 1203 36-37 -0.02712662466264959 1203 38-39 0.09317919106707251 1203 40-41 0.051235363218381735 1203 42-43 -0.036412710910219914 1203 44-45 -0.03583683381305747 1203 46-47 0.00679410076207887 1203 48-49 -0.0014776241116294386 1203 50-51 0.05833406577181677 1203 52-53 -0.02127559494707043 1203 54-55 -0.06248493927292742 1203 56-57 0.01745656989608335 1203 58-59 0.06986973639526894 1203 60-61 0.08985280689202568 1203 62-63 -0.05476497924656343 1203 64-65 -0.18467228937775815 1203 66-67 -0.04411873587297066 1203 68-69 -0.09798454189596129 1203 70-71 -0.070550484548761 1203 72-73 -0.11790882030972938 1203 74-75 -0.19430771065404429 1203 76-77 -0.03174083206273082 1203 78-79 0.09526874005808139 1203 80-81 -0.14494916338772867 1203 82-83 0.09600006787564297 1203 84-85 0.17267134933892692 1203 86-87 0.11265278888235741 1203 88-89 -0.2524345152451133 1203 90-91 0.15398325199824114 1204 1 0.0061792952023438374 1204 2 -0.06338033624131612 1204 3 -0.19018901602672145 1204 4 -0.2045791005256632 1204 5 -0.08154268660682362 1204 6 -0.011681942534558232 1204 7 0.004346935454648815 1204 8 -0.08421200058867129 1204 9 -0.13809240221079477 1204 10-11 -0.1710667441129914 1204 12-13 -0.17116082586830572 1204 14-15 -0.06643092057636579 1204 16-17 -0.16632879572928516 1204 18-19 -0.18084952761163464 1204 20-21 -0.17813365426361827 1204 22-23 -0.17555748428959106 1204 24-25 -0.11687317604069136 1204 26-27 -0.11591344944908144 1204 28-29 -0.01607573886514757 1204 30-31 -0.23938000909519985 1204 32-33 -0.11492488697879111 1204 34-35 -0.11684481152571635 1204 36-37 -0.07381582849004786 1204 38-39 -0.08488072545696213 1204 40-41 -0.11956351091963313 1204 42-43 -0.21701644306423162 1204 44-45 -0.18043012041699313 1204 46-47 -0.24816809413219687 1204 48-49 -0.1044318160167208 1204 50-51 -0.1264012736956488 1204 52-53 -0.17814457854247934 1204 54-55 -0.31112994229123814 1204 56-57 -0.21189002628317155 1204 58-59 -0.2994541084582423 1204 60-61 -0.20316771122021038 1204 62-63 -0.1041706940189755 1204 64-65 -0.17978461328679884 1204 66-67 -0.2583077410331498 1204 68-69 -0.12117925946763819 1204 70-71 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2401 30-31 -0.0061599371904037525 2401 32-33 -0.018371908875813858 2401 34-35 -0.044715076567719336 2401 36-37 0.08308263584799391 2401 38-39 0.08355134731519342 2401 40-41 0.07314884961573398 2401 42-43 0.06648361350030996 2401 44-45 0.06208011521781742 2401 46-47 0.025501001600225948 2401 48-49 0.08082879969511225 2401 50-51 -0.007170068336897373 2401 52-53 0.04419076730079752 2401 54-55 0.14279827254140542 2401 56-57 0.09047709178106089 2401 58-59 0.07647925281873569 2401 60-61 0.13040560594976824 2401 62-63 0.20962347867561704 2401 64-65 0.11065811208518994 2401 66-67 0.11281347437805778 2401 68-69 0.1439477671951579 2401 70-71 0.12345256307042973 2401 72-73 0.1925966539614663 2401 74-75 0.24641985742063355 2401 76-77 0.18535228382567936 2401 78-79 0.21810016185219894 2401 80-81 0.16136518927633148 2401 82-83 -0.0992857212330236 2401 84-85 -0.08353580075447553 2401 86-87 -0.004413204326382925 2401 88-89 0.10810909474295727 2401 90-91 -0.35083504592350323 2402 1 0.03352463012588203 2402 2 0.17465040652983532 2402 3 0.0416256059846134 2402 4 0.0670540473409531 2402 5 -0.028788241462827102 2402 6 -0.01958244911291729 2402 7 0.015715174749367122 2402 8 0.10907604951976424 2402 9 0.10857115912544657 2402 10-11 0.030102020357936965 2402 12-13 0.08446144310449455 2402 14-15 0.03871966235558233 2402 16-17 0.09219576282751518 2402 18-19 0.07785930329971791 2402 20-21 0.06850567867045498 2402 22-23 0.03956103866511995 2402 24-25 0.02555263300879318 2402 26-27 -0.0025211148377835 2402 28-29 0.01067207588653929 2402 30-31 0.07259713214394026 2402 32-33 0.02228904879233795 2402 34-35 0.10766117975924061 2402 36-37 0.0019894649945868537 2402 38-39 -0.23533960159227973 2402 40-41 0.1090254563210209 2402 42-43 0.05463981773727511 2402 44-45 0.05018663191791717 2402 46-47 0.11610414871233843 2402 48-49 0.09777887196480606 2402 50-51 0.06701049866171616 2402 52-53 0.0667985019407169 2402 54-55 0.09979915157845909 2402 56-57 0.10805968834797142 2402 58-59 -0.07673514688250549 2402 60-61 0.022385351589825575 2402 62-63 -0.08360532842809931 2402 64-65 -0.03848300306704289 2402 66-67 -0.09673969084275313 2402 68-69 -0.03999870986064735 2402 70-71 0.06716320297660872 2402 72-73 -0.12842868351686576 2402 74-75 -0.13441336548638105 2402 76-77 -0.11362673412803304 2402 78-79 0.10968879553097821 2402 80-81 0.15980444771959412 2402 82-83 0.17470859171054798 2402 84-85 0.1446564574377902 2402 86-87 0.19115843051650216 2402 88-89 0.41061307690288373 2402 90-91 0.5176196156346009 2403 1 0.0036225719545086577 2403 2 -0.05308660972681878 2403 3 0.07988320509676328 2403 4 0.09930474553900126 2403 5 0.020140427016983153 2403 6 0.08214209434662934 2403 7 -0.036690211708673814 2403 8 -0.047630876285133184 2403 9 1.8547521106171416E-4 2403 10-11 -0.04197165637694411 2403 12-13 0.032809630986562865 2403 14-15 0.002195855115331824 2403 16-17 4.0584108506891425E-4 2403 18-19 -0.013961446483918394 2403 20-21 0.03562757274530526 2403 22-23 0.05425231295932775 2403 24-25 0.012324548891626819 2403 26-27 0.0318824082364344 2403 28-29 0.013322145649254935 2403 30-31 -0.0025764753991097677 2403 32-33 0.10384734723961486 2403 34-35 0.06964156185271975 2403 36-37 0.10334981024860213 2403 38-39 0.07518426500133302 2403 40-41 0.11420642593147079 2403 42-43 0.06300616655335745 2403 44-45 0.07806510384953924 2403 46-47 0.07843557909358623 2403 48-49 0.09059339279805556 2403 50-51 0.056684478050513576 2403 52-53 0.05332806105638355 2403 54-55 -0.09567895026633977 2403 56-57 -0.03933668629115772 2403 58-59 0.051258115164898754 2403 60-61 -0.01042765876750451 2403 62-63 0.06576192097652012 2403 64-65 0.12200541526431152 2403 66-67 0.0292678681042986 2403 68-69 0.14687293835260817 2403 70-71 0.0558032424414705 2403 72-73 0.055345749535000266 2403 74-75 0.19646024124951111 2403 76-77 0.25457795426533636 2403 78-79 0.19361200307043447 2403 80-81 0.014306847082522722 2403 82-83 -0.11456821952165086 2403 84-85 -0.04743640465507326 2403 86-87 0.12495446629765183 2403 88-89 0.03337672117338286 2403 90-91 -0.7961058745614764 2404 1 0.026251729714623195 2404 2 -0.10806425413758092 2404 3 0.06176594558502302 2404 4 0.12205907647421554 2404 5 0.08685206540357626 2404 6 0.027792581389228133 2404 7 0.0925508083903992 2404 8 0.08167471278208893 2404 9 0.15748171197301275 2404 10-11 0.0923680466284651 2404 12-13 0.05479253267067463 2404 14-15 0.007033146979523508 2404 16-17 0.048653586551935746 2404 18-19 0.0032495818958437894 2404 20-21 -0.04253401279667912 2404 22-23 0.058862197448839026 2404 24-25 0.04587747335612846 2404 26-27 0.05805464652537751 2404 28-29 0.045423684316439505 2404 30-31 0.05380186250502561 2404 32-33 -0.04096635155543993 2404 34-35 0.09283603639723736 2404 36-37 0.052575368263781286 2404 38-39 0.17074702613386705 2404 40-41 0.021562970727345032 2404 42-43 0.12151912802281828 2404 44-45 0.055296237987626284 2404 46-47 0.1411098523931429 2404 48-49 0.0894968935264231 2404 50-51 0.12224787512586488 2404 52-53 0.022251855344094906 2404 54-55 0.035091816207263093 2404 56-57 0.10521108286526015 2404 58-59 0.07327378267256535 2404 60-61 -0.021999372901923664 2404 62-63 0.04233375534668937 2404 64-65 -0.0829194351232303 2404 66-67 0.03804501558941098 2404 68-69 0.1384141587241814 2404 70-71 0.028657944771886434 2404 72-73 0.06157989696516353 2404 74-75 0.03298013149617418 2404 76-77 0.14642302859382283 2404 78-79 0.07599364900599426 2404 80-81 0.12167991388686517 2404 82-83 0.10041709136444155 2404 84-85 0.13525307215204663 2404 86-87 0.21248808533032815 2404 88-89 0.17018876686393014 2404 90-91 0.22671052472551168 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 1.0 30 16.0 31 427.0 32 3348.0 33 5924.0 34 8008.0 35 9879.0 36 13973.0 37 20884.0 38 51675.0 39 337448.0 40 316095.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.348299677729464 12.528950940368228 23.41124794510198 32.711501436800326 2 30.569036839190904 17.835901110713007 25.321259893814535 26.273802156281555 3 27.304191736889344 19.458444111260334 20.21410046152223 33.02326369032809 4 27.790036968624864 20.84454680217487 19.553640979681585 31.811775249518675 5 28.000281367969382 21.476582629696306 19.87460888549626 30.648527116838046 6 27.835629000700813 24.520567216984205 22.503966506790608 25.139837275524375 7 26.667561138915012 23.451499196277606 22.427371892903015 27.453567771904364 8 25.89497158965087 25.094896558192364 22.836527815047454 26.17360403710931 9 25.162893817459924 24.73172345697024 23.882409030869713 26.22297369470012 10-11 25.585010903008815 23.440817634476954 23.470712981223897 27.503458481290334 12-13 25.769267844070036 22.988870333655516 23.429875546778728 27.811986275495716 14-15 25.99318985303734 22.986981520898084 23.42805186549569 27.591776760568887 16-17 26.345485998035635 22.689526077339718 22.961645377358735 28.003342547265913 18-19 26.3616386036854 22.831252165621525 22.95676051677917 27.85034871391391 20-21 26.292013057557988 22.962687480949043 23.068656389788426 27.676643071704543 22-23 26.419670747370645 22.33143323112034 22.98072889935624 28.268167122152775 24-25 26.338386667326663 22.673438603164346 23.074192565111932 27.91398216439705 26-27 26.449110173796825 22.931489504714218 23.018961074825643 27.600439246663317 28-29 26.582369170407382 22.543826969119866 22.853071209543586 28.020732650929165 30-31 26.457023497742192 22.686976622101046 22.98215573238223 27.87384414777453 32-33 26.49662173296859 22.794502488382058 23.05138261758017 27.65749316106918 34-35 26.66415918058937 22.526068340189738 22.844765342960287 27.9650071362606 36-37 26.640698302188238 22.63623088261759 22.985093490233357 27.737977324960816 38-39 26.796573033192832 22.924557507614345 22.94514920873568 27.333720250457144 40-41 26.7760582440815 22.63201914364705 22.836683665172036 27.755238947099414 42-43 26.64417655240367 22.832748049638464 22.944873185194975 27.578202212762886 44-45 26.781856608884453 23.06050943736264 22.878261025599816 27.27937292815309 46-47 26.897521281087716 22.79962543007608 22.817442722286994 27.485410566549213 48-49 26.861946726149633 23.059423586860447 22.83175974518015 27.24686994180977 50-51 26.89268272995116 23.203688008143754 22.82869865870229 27.07493060320279 52-53 27.06558785540743 22.91715268176016 22.70046107422138 27.31679838861103 54-55 27.047405440285416 23.095391733367425 22.72745118370149 27.129751642645665 56-57 27.16439069707498 23.287062146589268 22.752965562423142 26.79558159391261 58-59 27.346213420368425 22.961728531651637 22.6528645977393 27.03919345024064 60-61 27.38178716716896 23.162404638995813 22.59943902366246 26.856369170172766 62-63 27.277040297040813 23.390882512357553 22.682477484722863 26.649599705878774 64-65 27.549850680921622 22.958011682239533 22.486462180635407 27.005675456203438 66-67 27.54452843232526 23.21609088952514 22.526723581318603 26.712657096831 68-69 27.51713107285891 23.299345912724363 22.594540155452147 26.588982858964584 70-71 27.857235756496856 23.06882564765365 22.288877508718024 26.785061087131467 72-73 27.80060421568334 23.397111655463405 22.24790356514906 26.5543805637042 74-75 27.88891242189982 23.488033463941544 22.178597903208725 26.444456210949912 76-77 28.04491234132886 23.07079957296867 22.02852223710112 26.855765848601347 78-79 27.986603249518144 23.342811705300804 21.95220262218672 26.718382422994335 80-81 28.20112499386651 23.316189295161454 21.785269806716954 26.697415904255084 82-83 28.65654559531424 22.64898284354978 21.433220004263866 27.261251556872118 84-85 29.22777666836776 22.45593793220765 21.035153505365848 27.281131894058742 86-87 29.5385294303066 22.165717527896277 20.41839157753168 27.877361464265448 88-89 29.08143906729675 21.549791314545892 20.73057155669001 28.638198061467353 90-91 24.347385317225648 24.241784235870576 23.861620342992314 27.549210103911463 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.5 5 2.0 6 1.0 7 0.0 8 0.0 9 0.5 10 1.5 11 3.5 12 6.5 13 11.5 14 20.5 15 34.5 16 54.5 17 77.5 18 109.5 19 159.5 20 228.0 21 318.5 22 449.0 23 602.5 24 799.0 25 1069.0 26 1419.5 27 1853.0 28 2330.0 29 2900.0 30 3586.0 31 4327.0 32 5142.0 33 6019.5 34 7123.5 35 8534.0 36 10102.0 37 11849.0 38 13875.5 39 16233.5 40 18591.5 41 21239.5 42 24141.5 43 26850.0 44 29684.5 45 32686.0 46 35839.5 47 38766.0 48 40971.0 49 42654.5 50 44132.0 51 45524.5 52 46452.5 53 46952.0 54 47397.5 55 47652.5 56 47538.0 57 47075.0 58 46579.5 59 46039.5 60 45326.0 61 44193.0 62 42922.5 63 41365.5 64 39314.5 65 36747.5 66 33472.5 67 30441.5 68 27348.5 69 23950.5 70 20782.5 71 17769.0 72 14815.5 73 12042.0 74 9561.5 75 7519.5 76 5964.0 77 4609.5 78 3498.5 79 2582.5 80 1831.0 81 1301.5 82 948.0 83 682.5 84 463.0 85 323.0 86 242.0 87 176.5 88 124.0 89 79.5 90 43.0 91 26.5 92 20.5 93 15.0 94 9.5 95 6.5 96 3.5 97 2.5 98 3.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01993023116462892 3 1.302629487884243E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.05976481698786732 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 1.3516582142972998E-4 64-65 2.9582691758705816E-4 66-67 0.0 68-69 3.62273556385162E-4 70-71 2.0321692390542286E-4 72-73 2.3044023302116364E-4 74-75 2.6474567207012583E-4 76-77 0.0 78-79 7.328584410635242E-4 80-81 8.921362648930997E-4 82-83 0.0 84-85 7.396012070291698E-4 86-87 0.0 88-89 0.0017246434299708535 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 5964.0 32-33 13518.0 34-35 15833.0 36-37 18100.0 38-39 20158.0 40-41 22378.0 42-43 24447.0 44-45 25961.0 46-47 27661.0 48-49 28765.0 50-51 30125.0 52-53 30424.0 54-55 30947.0 56-57 31652.0 58-59 31702.0 60-61 32068.0 62-63 32061.0 64-65 31474.0 66-67 30757.0 68-69 30220.0 70-71 29310.0 72-73 28414.0 74-75 27153.0 76-77 25868.0 78-79 24681.0 80-81 23360.0 82-83 21899.0 84-85 20386.0 86-87 18991.0 88-89 17494.0 90-91 15907.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.86175114110016 #Duplication Level Percentage of deduplicated Percentage of total 1 99.86560103551382 99.72753798164875 2 0.13066258500512298 0.26096389094468875 3 0.0034314724811552646 0.010280185528819815 4 3.0490699988553973E-4 0.0012179418777499688 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0022144701294032135 0.0 0.0 0.0 0.0 2 0.0022144701294032135 0.0 0.0 0.0 0.0 3 0.0022144701294032135 0.0 0.0 0.0 0.0 4 0.0022144701294032135 0.0 0.0 0.0 0.0 5 0.0022144701294032135 0.0 0.0 0.0 1.302629487884243E-4 6 0.0022144701294032135 0.0 0.0 0.0 2.605258975768486E-4 7 0.0026052589757684865 0.0 0.0 0.0 2.605258975768486E-4 8 0.011723665390958189 0.0 0.0 0.0 2.605258975768486E-4 9 0.012505243083688734 0.0 0.0 0.0 2.605258975768486E-4 10-11 0.012505243083688734 0.0 0.0 0.0 3.9078884636527294E-4 12-13 0.012505243083688734 0.0 0.0 0.0 3.9078884636527294E-4 14-15 0.012635506032477158 0.0 0.0 0.0 3.9078884636527294E-4 16-17 0.012635506032477158 0.0 0.0 0.0 3.9078884636527294E-4 18-19 0.012635506032477158 0.0 0.0 0.0 4.559203207594851E-4 20-21 0.01270063750687137 0.0 0.0 0.0 5.210517951536972E-4 22-23 0.012765768981265583 0.0 0.0 0.0 6.513147439421216E-4 24-25 0.012830900455659794 0.0 0.0 0.0 6.513147439421216E-4 26-27 0.012896031930054007 0.0 0.0 0.0 6.513147439421216E-4 28-29 0.013026294878842432 0.0 0.0 0.0 6.513147439421216E-4 30-31 0.013351952250813492 0.0 0.0 0.0 6.513147439421216E-4 32-33 0.013417083725207704 0.0 0.0 0.0 6.513147439421216E-4 34-35 0.013547346673996128 0.0 0.0 0.0 6.513147439421216E-4 36-37 0.013677609622784553 0.0 0.0 0.0 6.513147439421216E-4 38-39 0.013938135520361402 0.0 0.0 0.0 6.513147439421216E-4 40-41 0.014068398469149826 0.0 0.0 0.0 6.513147439421216E-4 42-43 0.014198661417938251 0.0 6.513147439421216E-5 0.0 8.46709167124758E-4 44-45 0.014328924366726675 0.0 1.302629487884243E-4 0.0 9.118406415189702E-4 46-47 0.014328924366726675 0.0 1.302629487884243E-4 0.0 0.0010421035903073945 48-49 0.014654581738697735 0.0 1.302629487884243E-4 0.0 0.0010421035903073945 50-51 0.015045370585063007 0.0 1.302629487884243E-4 0.0 0.0010421035903073945 52-53 0.015175633533851433 0.0 1.302629487884243E-4 0.0 0.0010421035903073945 54-55 0.015371027957034069 6.513147439421216E-5 1.302629487884243E-4 0.0 0.0010421035903073945 56-57 0.015501290905822493 1.302629487884243E-4 1.302629487884243E-4 0.0 0.0011723665390958188 58-59 0.01576181680339934 1.302629487884243E-4 1.302629487884243E-4 0.0 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