##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-36B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 436963 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 57 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.59440959532043 40.0 40.0 40.0 40.0 40.0 2 39.332190597373234 40.0 40.0 40.0 40.0 40.0 3 39.30839910930674 40.0 40.0 40.0 40.0 40.0 4 39.19593192100933 40.0 40.0 40.0 40.0 40.0 5 39.21750811853635 40.0 40.0 40.0 40.0 40.0 6 39.36881612401965 40.0 40.0 40.0 40.0 40.0 7 39.39751420600829 40.0 40.0 40.0 40.0 40.0 8 39.42606582250671 40.0 40.0 40.0 40.0 40.0 9 39.40608243718576 40.0 40.0 40.0 40.0 40.0 10-11 39.40486036575179 40.0 40.0 40.0 40.0 40.0 12-13 39.32602531564457 40.0 40.0 40.0 40.0 40.0 14-15 39.370010733174205 40.0 40.0 40.0 40.0 40.0 16-17 39.47592816783114 40.0 40.0 40.0 40.0 40.0 18-19 39.50270389026073 40.0 40.0 40.0 40.0 40.0 20-21 39.488309078800725 40.0 40.0 40.0 40.0 40.0 22-23 39.52662353563116 40.0 40.0 40.0 40.0 40.0 24-25 39.5055736984596 40.0 40.0 40.0 40.0 40.0 26-27 39.47678407553958 40.0 40.0 40.0 40.0 40.0 28-29 39.49484510130148 40.0 40.0 40.0 40.0 40.0 30-31 39.477544105720135 40.0 40.0 40.0 40.0 40.0 32-33 39.48383551657855 40.0 40.0 40.0 40.0 40.0 34-35 39.4696194053854 40.0 40.0 40.0 40.0 40.0 36-37 39.45005351404717 40.0 40.0 40.0 40.0 40.0 38-39 39.39410857309282 40.0 40.0 40.0 40.0 40.0 40-41 39.44210804801513 40.0 40.0 40.0 40.0 40.0 42-43 39.42357197920086 40.0 40.0 40.0 40.0 40.0 44-45 39.326694599288416 40.0 40.0 40.0 40.0 40.0 46-47 39.30995928344802 40.0 40.0 40.0 40.0 40.0 48-49 39.38997201183584 40.0 40.0 40.0 40.0 40.0 50-51 39.336479069589394 40.0 40.0 40.0 40.0 40.0 52-53 39.29910878402998 40.0 40.0 40.0 40.0 40.0 54-55 39.20925248772606 40.0 40.0 40.0 40.0 40.0 56-57 39.20728223164839 40.0 40.0 40.0 40.0 40.0 58-59 39.17193517422441 40.0 40.0 40.0 40.0 40.0 60-61 39.08432251939831 40.0 40.0 40.0 40.0 40.0 62-63 39.39268986917669 40.0 40.0 40.0 40.0 40.0 64-65 39.398981427090945 40.0 40.0 40.0 40.0 40.0 66-67 39.39819943961465 40.0 40.0 40.0 40.0 40.0 68-69 39.39744246165415 40.0 40.0 40.0 40.0 40.0 70-71 39.39788472935022 40.0 40.0 40.0 40.0 40.0 72-73 39.42235084548068 40.0 40.0 40.0 40.0 40.0 74-75 39.40008338169221 40.0 40.0 40.0 40.0 40.0 76-77 39.38623357922327 40.0 40.0 40.0 40.0 40.0 78-79 39.40671642803807 40.0 40.0 40.0 40.0 40.0 80-81 39.400480514077174 40.0 40.0 40.0 40.0 40.0 82-83 39.377516443792246 40.0 40.0 40.0 40.0 40.0 84-85 39.387045310431446 40.0 40.0 40.0 40.0 40.0 86-87 39.38998912360995 40.0 40.0 40.0 40.0 40.0 88-89 39.412205744222504 40.0 40.0 40.0 40.0 40.0 90-91 39.482228226633 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05544525078492768 1101 2 -0.009217061899931878 1101 3 0.024681299723184225 1101 4 0.04115762525333366 1101 5 0.04956241371793624 1101 6 -0.08718677176079126 1101 7 -0.12209069944101714 1101 8 0.06624286581206462 1101 9 0.06965090453712008 1101 10-11 -0.14119892520125887 1101 12-13 -0.012130853305556855 1101 14-15 -0.03692744491815603 1101 16-17 -0.0029224217373879924 1101 18-19 0.0172057125986953 1101 20-21 0.057529617524828325 1101 22-23 -0.03995803400226805 1101 24-25 0.16135164454728113 1101 26-27 -0.026505919210201512 1101 28-29 0.07698698056943698 1101 30-31 0.04041083066511675 1101 32-33 -0.01742941450854829 1101 34-35 0.0068208247212808715 1101 36-37 0.003582580697148785 1101 38-39 -0.041785350262607324 1101 40-41 0.05680660980584662 1101 42-43 -0.07506460175477514 1101 44-45 -0.027057000251332397 1101 46-47 -0.05758909470605289 1101 48-49 0.012291768867129349 1101 50-51 -0.08961460355450157 1101 52-53 -0.03149009806540448 1101 54-55 -0.12754234797657915 1101 56-57 -0.16393143430790502 1101 58-59 -0.09950783674154451 1101 60-61 0.09404974730465909 1101 62-63 -0.11705264110986491 1101 64-65 -0.1096061544140241 1101 66-67 -0.189204096191105 1101 68-69 -0.13378635507886827 1101 70-71 -0.14977071981787304 1101 72-73 0.013995294590991136 1101 74-75 -0.19172066354376227 1101 76-77 0.04288434556953291 1101 78-79 0.08520828439442596 1101 80-81 0.08193831355850278 1101 82-83 -0.048214585321034065 1101 84-85 -0.10838957796919857 1101 86-87 -0.1245492621087223 1101 88-89 0.07178545089576005 1101 90-91 -0.6097158242069369 1102 1 0.04825936083102533 1102 2 0.06808578298151957 1102 3 0.20418707083579335 1102 4 0.15066139209802287 1102 5 -0.10970614639241205 1102 6 -0.05708148874636976 1102 7 0.08520955168196309 1102 8 0.038813869950288904 1102 9 -0.15479762704189426 1102 10-11 0.16156637502628257 1102 12-13 0.03658464675928741 1102 14-15 -0.04652140381887904 1102 16-17 0.029010282020024647 1102 18-19 -0.031733793879809014 1102 20-21 -0.03595179583774666 1102 22-23 -0.020092202054236452 1102 24-25 -0.06685783681891877 1102 26-27 -0.11820412362194332 1102 28-29 0.07848428659756479 1102 30-31 0.06309442750073657 1102 32-33 0.04304271143846705 1102 34-35 0.0036109313073353633 1102 36-37 -0.07972868073182582 1102 38-39 -0.07332545203675522 1102 40-41 -0.008377334730155894 1102 42-43 0.09196600633124774 1102 44-45 -0.16978249234276888 1102 46-47 -0.11801774216188221 1102 48-49 -0.030202889889856976 1102 50-51 0.06841600594921005 1102 52-53 -0.31132124940975103 1102 54-55 0.14732169912999638 1102 56-57 0.023588870602111456 1102 58-59 0.0278373863800212 1102 60-61 -0.0141446354294672 1102 62-63 -0.08458897302174506 1102 64-65 -0.01183668406000038 1102 66-67 -0.06868788182958951 1102 68-69 -0.030819819433972384 1102 70-71 -0.20341609133161143 1102 72-73 -0.31218958768871374 1102 74-75 0.02627241604101016 1102 76-77 0.16522273941015442 1102 78-79 0.20720944719255385 1102 80-81 -0.13035830361616263 1102 82-83 -0.15756505976241897 1102 84-85 -0.8637025202087472 1102 86-87 -0.13100047615487398 1102 88-89 -0.1191615662089589 1102 90-91 0.45695084245972595 1103 1 -0.021913323627373416 1103 2 0.05646010707013005 1103 3 -0.15738627645183811 1103 4 -0.14670473383952753 1103 5 0.008661200546363546 1103 6 -0.03658611110813581 1103 7 -0.26612482822384464 1103 8 0.012004171712021616 1103 9 0.08397788316219135 1103 10-11 -0.0030158681273846355 1103 12-13 -0.14108847377097788 1103 14-15 0.026921819624348586 1103 16-17 -0.06753602881200038 1103 18-19 -0.07715503930105427 1103 20-21 0.010795108052022329 1103 22-23 -0.008963648068537111 1103 24-25 -0.10399371681194225 1103 26-27 0.051140024293630404 1103 28-29 0.02664406332876723 1103 30-31 -0.002525666690793571 1103 32-33 -0.028988956359931706 1103 34-35 -0.0028384363104478894 1103 36-37 0.01352317279248183 1103 38-39 0.12435245854695864 1103 40-41 0.05502707933091244 1103 42-43 0.087679069634504 1103 44-45 0.09637486991556443 1103 46-47 0.007827543372307844 1103 48-49 0.1516605333385428 1103 50-51 0.021080181576145662 1103 52-53 0.1978945855228389 1103 54-55 0.048891794354503304 1103 56-57 0.04817516606297545 1103 58-59 0.008912085947848425 1103 60-61 0.07415220604834616 1103 62-63 0.05863728721549677 1103 64-65 -0.07210411928465987 1103 66-67 0.1884242348499754 1103 68-69 0.10015509826210689 1103 70-71 0.3386497435888245 1103 72-73 -0.03000176547413247 1103 74-75 0.31005558486204876 1103 76-77 0.2615400862649011 1103 78-79 0.2253462083860498 1103 80-81 0.22124209575493836 1103 82-83 0.3882732195570142 1103 84-85 0.3286959864420993 1103 86-87 0.11330101970440865 1103 88-89 0.41221098281064883 1103 90-91 -0.20971582420694546 1104 1 0.03004497448476684 1104 2 -0.05346757208832287 1104 3 0.05503036673403017 1104 4 -0.08554196498445776 1104 5 -0.06369721027358821 1104 6 0.007745625531292433 1104 7 0.15585319375417583 1104 8 0.021847320016703975 1104 9 -0.10289165755350638 1104 10-11 -0.015113172532380759 1104 12-13 -0.1675372386812839 1104 14-15 -0.008693846760550628 1104 16-17 -0.04753039342175214 1104 18-19 0.03899035376741722 1104 20-21 -0.15948760069223056 1104 22-23 -0.04758485592051187 1104 24-25 0.017693143336451556 1104 26-27 -0.05014672314696611 1104 28-29 -0.02202692380375737 1104 30-31 0.03370989262169388 1104 32-33 0.13151127044309874 1104 34-35 -0.044499866153429934 1104 36-37 -0.047196332469390256 1104 38-39 -0.04069523163283151 1104 40-41 -0.13269897536176956 1104 42-43 -0.14502207824624236 1104 44-45 -0.20958845322501674 1104 46-47 -0.06093957927170379 1104 48-49 -0.15289524054493597 1104 50-51 0.1365723603930391 1104 52-53 0.01783590620051001 1104 54-55 -0.05685934115233948 1104 56-57 0.03914984593524906 1104 58-59 0.06178556185998474 1104 60-61 -0.06995692317204316 1104 62-63 -0.09661739873427422 1104 64-65 0.06857648301706831 1104 66-67 0.07415840384295791 1104 68-69 0.01819482756319246 1104 70-71 -0.0823774925830989 1104 72-73 -0.05650926066729056 1104 74-75 0.01723897761704052 1104 76-77 -0.05997957572237311 1104 78-79 -0.22508876020761193 1104 80-81 0.1541244094965748 1104 82-83 -0.2081211481686438 1104 84-85 0.10819832059452494 1104 86-87 -0.1451852174904431 1104 88-89 -0.263714943115275 1104 90-91 0.45695084245972595 1201 1 0.06252041945545983 1201 2 -0.08610455872353384 1201 3 -0.060015150415146934 1201 4 0.055781924660308846 1201 5 -0.06778031510437899 1201 6 0.03967517108394958 1201 7 0.038576730513952384 1201 8 -0.06882490219778248 1201 9 0.13296195784642606 1201 10-11 -0.03265019133667124 1201 12-13 0.03210179925454071 1201 14-15 -0.049992808855357396 1201 16-17 -0.02474466312112611 1201 18-19 -0.00874799329927356 1201 20-21 0.0043602103485866905 1201 22-23 -0.0720932331091646 1201 24-25 -0.0455699147308124 1201 26-27 -0.014008738713457092 1201 28-29 -0.04770787066072302 1201 30-31 -0.11352976418582728 1201 32-33 -0.01382285860152166 1201 34-35 -0.020568645636053873 1201 36-37 0.0684038927171926 1201 38-39 -0.0017917120748052184 1201 40-41 -0.10181740774716985 1201 42-43 -0.00630937949782151 1201 44-45 -0.02035456143181591 1201 46-47 -0.14194648734250848 1201 48-49 0.1298497066692761 1201 50-51 -0.06790580072413377 1201 52-53 0.15871240249463625 1201 54-55 0.04555788260553584 1201 56-57 0.15922571228317395 1201 58-59 -0.1519232763517948 1201 60-61 -0.05272627872722779 1201 62-63 -0.08538670061091835 1201 64-65 -0.0835900166643313 1201 66-67 -0.053721269242188896 1201 68-69 -0.19670809849598925 1201 70-71 -0.18363965150341954 1201 72-73 -0.02211440194471237 1201 74-75 -0.15817797202997497 1201 76-77 0.13232133480888564 1201 78-79 0.09490610291577184 1201 80-81 -0.051641613125191554 1201 82-83 0.03379274068628746 1201 84-85 0.3232503417441137 1201 86-87 0.243025196578877 1201 88-89 -0.12945568385602257 1201 90-91 -0.7298623443534638 1202 1 -0.10181475332167622 1202 2 -0.04408443721924016 1202 3 -0.11419662066897729 1202 4 0.07132825039063562 1202 5 0.0762668343491768 1202 6 0.05954826174536976 1202 7 0.08505737813648295 1202 8 0.09631010788208272 1202 9 0.007534911607372408 1202 10-11 -0.10087522133056837 1202 12-13 0.09295085521449664 1202 14-15 0.020616719035899678 1202 16-17 -0.13065076549757038 1202 18-19 -0.10444456833042892 1202 20-21 -0.20406974724365767 1202 22-23 -0.0255648136340767 1202 24-25 0.015842849089246158 1202 26-27 -0.03039857886883368 1202 28-29 -0.006265106117268715 1202 30-31 -0.059241830946312746 1202 32-33 0.0019040068005224953 1202 34-35 -0.018226972971390865 1202 36-37 -0.25487627394789314 1202 38-39 -0.07881253537696864 1202 40-41 -0.18752397795024933 1202 42-43 -0.19422624267131994 1202 44-45 -2.738744570507379E-4 1202 46-47 -0.02708026531713159 1202 48-49 -0.014587017903963329 1202 50-51 -0.11676662027467444 1202 52-53 0.057195363085746465 1202 54-55 -0.10675044516786869 1202 56-57 -0.13743929412736833 1202 58-59 -0.21323923638254882 1202 60-61 -0.2312948373324346 1202 62-63 -0.2928093387414137 1202 64-65 -0.2511766439677814 1202 66-67 -0.3739048609075013 1202 68-69 0.007675640072996259 1202 70-71 -0.21072478525739058 1202 72-73 -0.3439608480777352 1202 74-75 -0.15021341504053964 1202 76-77 -0.40393982988607036 1202 78-79 -0.5867578049989746 1202 80-81 -0.15118758306612534 1202 82-83 0.09473320584361744 1202 84-85 -0.6792688179230595 1202 86-87 -0.29708855077704044 1202 88-89 -0.8446673240676574 1202 90-91 -1.15477076926188 1203 1 0.04488048862098282 1203 2 0.07007881159792362 1203 3 0.08289061837050582 1203 4 0.13595402154649605 1203 5 -0.027702435817275273 1203 6 0.1138129847455005 1203 7 -0.002933474123402391 1203 8 0.07772431015943226 1203 9 -0.03723423804993331 1203 10-11 0.10248671010246824 1203 12-13 0.09110761297056058 1203 14-15 -0.0786937769003444 1203 16-17 -0.0017593812171696754 1203 18-19 -0.09635402213640276 1203 20-21 0.10349836439164051 1203 22-23 -0.024320008879442412 1203 24-25 -0.022606417242826637 1203 26-27 0.017262790416396 1203 28-29 0.039669421808675054 1203 30-31 -0.06936186079970952 1203 32-33 -0.006699781178490127 1203 34-35 0.08692459422147181 1203 36-37 0.05024120979224023 1203 38-39 0.014676419053898826 1203 40-41 0.06719124068786186 1203 42-43 -0.013892964292175236 1203 44-45 -0.032085107322885165 1203 46-47 0.1167839003040001 1203 48-49 0.13495030313785605 1203 50-51 0.0035190157332820604 1203 52-53 0.10106730184593715 1203 54-55 -0.09942245781436299 1203 56-57 -0.036171586752843154 1203 58-59 0.1324191965275574 1203 60-61 0.24067197212870184 1203 62-63 -0.0272851807708534 1203 64-65 0.04396725983596639 1203 66-67 0.2628405784588992 1203 68-69 0.1509189794110668 1203 70-71 -0.18121836854570716 1203 72-73 0.039372204474638295 1203 74-75 0.0030608486274559255 1203 76-77 0.19876347026551855 1203 78-79 -0.2494698115784999 1203 80-81 -0.011035752405064159 1203 82-83 0.06071649145110314 1203 84-85 -0.2310609207638663 1203 86-87 -0.2633170422594233 1203 88-89 -0.32986360926393843 1203 90-91 0.19888632633069392 1204 1 0.06609741874746078 1204 2 -0.12625404771385007 1204 3 -0.1368905073276423 1204 4 -0.05080772422680724 1204 5 -0.2919206780903778 1204 6 -0.015442389216289598 1204 7 -0.17599118796552204 1204 8 -0.08491592121804814 1204 9 -0.04769293978045397 1204 10-11 -0.19128024990797599 1204 12-13 -0.17168719065399074 1204 14-15 -0.164292452387933 1204 16-17 -0.009795764089190584 1204 18-19 -0.010002408058646495 1204 20-21 -0.03854734777422664 1204 22-23 -0.03222174214957363 1204 24-25 -0.22685749753108553 1204 26-27 -0.02559975020175642 1204 28-29 -0.1110927911163131 1204 30-31 0.0069762196190765735 1204 32-33 -0.1265389314229921 1204 34-35 -0.06699493723149885 1204 36-37 -0.1426361247829604 1204 38-39 -0.007163877957772513 1204 40-41 -0.040414381471578054 1204 42-43 -0.033562282850923 1204 44-45 0.00809172133531888 1204 46-47 0.043355996653154705 1204 48-49 -0.08737372702381663 1204 50-51 -0.12480545571411739 1204 52-53 -0.10254408022178296 1204 54-55 0.07522016445637547 1204 56-57 0.1885425212438392 1204 58-59 -0.06314917925273988 1204 60-61 -0.17240759321122567 1204 62-63 -0.07224380167453859 1204 64-65 -0.1507772681305255 1204 66-67 -0.15074783777974687 1204 68-69 -0.0036187755889045548 1204 70-71 0.16494451446730096 1204 72-73 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36-37 0.03406193347611719 2401 38-39 0.16008816291884642 2401 40-41 0.11534155535279922 2401 42-43 0.1672421044759318 2401 44-45 0.16437569838636534 2401 46-47 0.045327661786998874 2401 48-49 0.11526601292958105 2401 50-51 0.16438811911830697 2401 52-53 0.030215732706679432 2401 54-55 0.13795446861577432 2401 56-57 0.1838520614621757 2401 58-59 0.07786746903098418 2401 60-61 0.21190176517990977 2401 62-63 0.223979382254484 2401 64-65 0.296953296700174 2401 66-67 0.11152126999415657 2401 68-69 0.146395567997331 2401 70-71 0.26091647827512077 2401 72-73 0.2289629783170426 2401 74-75 0.17224582174774383 2401 76-77 0.2019762052961056 2401 78-79 0.3324463051333879 2401 80-81 0.3143414900705608 2401 82-83 0.3001718855050015 2401 84-85 0.20295771876606494 2401 86-87 0.42554644096210126 2401 88-89 -0.0836033246474912 2401 90-91 -0.24555811811374895 2402 1 0.0015227611972576938 2402 2 0.06550265472412775 2402 3 0.11415666159535931 2402 4 0.11675550331132456 2402 5 0.10464171211761197 2402 6 0.05756631921968847 2402 7 0.20320900481278414 2402 8 0.07141473002635479 2402 9 -0.028242985696927292 2402 10-11 0.14629339616360681 2402 12-13 0.054341323120446816 2402 14-15 0.051051242779344363 2402 16-17 0.028906708312831597 2402 18-19 0.08038267268245392 2402 20-21 0.11190174265438202 2402 22-23 0.046542978205444285 2402 24-25 0.0780086359577652 2402 26-27 0.12075077624299269 2402 28-29 0.05537492104866004 2402 30-31 0.1054602166909504 2402 32-33 0.09324372663790825 2402 34-35 0.006310036095925398 2402 36-37 0.02364134510400362 2402 38-39 -0.3185701616321239 2402 40-41 0.004362730949488025 2402 42-43 0.18003605500699393 2402 44-45 0.2529912305961659 2402 46-47 0.06654245978110396 2402 48-49 0.18260907208960475 2402 50-51 0.2915754421831238 2402 52-53 0.0810567684128074 2402 54-55 0.23338447025592757 2402 56-57 0.24851376215367083 2402 58-59 -0.0970334538942339 2402 60-61 0.3584295016554009 2402 62-63 0.1251582502719799 2402 64-65 0.05540518424858476 2402 66-67 0.12265489086345838 2402 68-69 0.09732634747238933 2402 70-71 0.13353149497970662 2402 72-73 0.053399412312749917 2402 74-75 0.16218995237403533 2402 76-77 -0.07275964362570875 2402 78-79 -0.026864827678686254 2402 80-81 0.020088283531897844 2402 82-83 -0.0970709290789813 2402 84-85 -0.10866197670667077 2402 86-87 -0.545768603716418 2402 88-89 -0.8519133109746662 2402 90-91 -0.1716205861117075 2403 1 -0.09348332893672051 2403 2 0.015563556063952433 2403 3 -3.378572840588845E-4 2403 4 0.026621520363704576 2403 5 0.25811308848789594 2403 6 0.0648744799992258 2403 7 0.09358659311973128 2403 8 0.04218208690820546 2403 9 -0.05991168240825573 2403 10-11 0.020836636114886176 2403 12-13 -0.13079874996115848 2403 14-15 0.08881007347362413 2403 16-17 -0.028340551126873947 2403 18-19 0.06454832432679325 2403 20-21 0.10337555507825869 2403 22-23 0.15251130950873204 2403 24-25 0.05364810002597409 2403 26-27 0.01721849853288404 2403 28-29 0.005435822388498934 2403 30-31 0.012890180150144204 2403 32-33 0.03403808145089471 2403 34-35 0.14725114733101918 2403 36-37 0.10740645471094012 2403 38-39 0.10151714903568632 2403 40-41 0.05339264895816598 2403 42-43 0.07464917473076582 2403 44-45 0.1237747211387159 2403 46-47 0.1537275502669999 2403 48-49 0.15036286053525316 2403 50-51 0.09770349517175703 2403 52-53 0.05538095787049002 2403 54-55 0.03656988331950828 2403 56-57 -0.04901503445468336 2403 58-59 0.09987517399466839 2403 60-61 -0.11203950417520758 2403 62-63 0.07772992029975256 2403 64-65 -0.061713848356802714 2403 66-67 0.1265733829117579 2403 68-69 0.0014983309052070126 2403 70-71 0.15722333613251038 2403 72-73 0.16624162140367815 2403 74-75 0.22614110266407295 2403 76-77 0.0794520228509441 2403 78-79 0.2733566562637293 2403 80-81 0.042612745378711736 2403 82-83 -0.1351195230229294 2403 84-85 0.18841403377347632 2403 86-87 -0.15774014529960567 2403 88-89 -0.01993436197690812 2403 90-91 0.45695084245972595 2404 1 0.031714446260075135 2404 2 0.009700487231292243 2404 3 -0.001338975199061565 2404 4 0.09139384946453077 2404 5 0.04153791287512831 2404 6 0.04359999885760146 2404 7 0.09511583700728465 2404 8 0.02350470876348254 2404 9 0.07651312239188712 2404 10-11 0.15246332940064633 2404 12-13 0.14680979697422458 2404 14-15 0.09244311587821841 2404 16-17 0.05407176581545059 2404 18-19 0.06444023260988985 2404 20-21 0.07194770702474784 2404 22-23 0.05601805145753502 2404 24-25 0.06008644893588411 2404 26-27 0.07012975668419585 2404 28-29 -0.014181010611252987 2404 30-31 0.032528776582829266 2404 32-33 -0.06704051450952164 2404 34-35 0.031230909983442245 2404 36-37 0.11896597426378008 2404 38-39 0.10703202834815073 2404 40-41 0.09838539694659687 2404 42-43 0.13142195004964918 2404 44-45 0.16918359267102545 2404 46-47 0.15124605852371786 2404 48-49 0.06552736868373188 2404 50-51 0.03097325597639866 2404 52-53 0.11370560793759665 2404 54-55 -0.06001058472293863 2404 56-57 0.08324482459212135 2404 58-59 -0.0233068454888965 2404 60-61 0.021840724251141808 2404 62-63 0.06713812636520089 2404 64-65 -0.014479079012502893 2404 66-67 0.11414081096175721 2404 68-69 0.20358107031381678 2404 70-71 0.17611393778964413 2404 72-73 0.049660902229696546 2404 74-75 0.18386586623408618 2404 76-77 -0.06498303765327762 2404 78-79 0.15636501188363638 2404 80-81 -0.057449750627924345 2404 82-83 0.12458891768936553 2404 84-85 -0.08462474654163543 2404 86-87 0.2708553332958772 2404 88-89 0.22742837411499295 2404 90-91 0.05695084245972737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 14.0 31 264.0 32 1830.0 33 3255.0 34 4356.0 35 5412.0 36 7783.0 37 11469.0 38 28182.0 39 192347.0 40 182051.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.0056846918389 11.83303849525017 22.775383728141744 33.385893084769194 2 30.829520197222493 16.670290958117686 24.533324787635596 27.96686405702422 3 28.166458029627222 18.474333067101792 19.51149181967352 33.847717083597466 4 28.221382588457146 19.640106828266923 18.72492636676332 33.41358421651261 5 28.640411201863774 20.30652480873667 18.97597737108176 32.07708661831779 6 28.581825005778523 22.778358808411696 21.580316868933984 27.0594993168758 7 27.693191414376045 22.00277826726748 21.197218071095264 29.10681224726121 8 26.837283705943065 23.212949380153468 21.39929467712369 28.550472236779772 9 26.498124555168285 23.189148737993833 22.289759087153833 28.022967619684046 10-11 26.981575099035844 21.91409798999 21.587296864951952 29.517030046022203 12-13 27.256197893185462 21.545645741172596 21.522989360655252 29.67516700498669 14-15 27.500726606142855 21.602401118630183 21.41096614587505 29.485906129351914 16-17 28.058096452102355 21.28441080823777 20.89078480328998 29.766707936369897 18-19 27.984062723846183 21.552168032533647 20.884605790421613 29.579163453198554 20-21 27.742394664994517 21.741085629675737 20.9705398397576 29.545979865572143 22-23 27.957401427580823 21.148586951297936 20.949943130196377 29.944068490924863 24-25 28.077091195364368 21.34871831253447 21.034160787068927 29.540029705032232 26-27 28.05054432526324 21.75859283280278 21.025121120094838 29.16574172183915 28-29 28.17835835070704 21.39139927179189 20.913555609971553 29.516686767529514 30-31 28.15443994237452 21.500848476803913 21.149099630514957 29.195611950306606 32-33 28.108439285163104 21.642950552976302 21.284975127380072 28.963635034480518 34-35 28.32778993175984 21.375949344574284 21.08781103818722 29.20844968547866 36-37 28.24562214448018 21.429475046588458 21.319998248371228 29.004904560560128 38-39 28.321046513523445 21.68679080137485 21.381072821822094 28.61108986327961 40-41 28.431142475958826 21.273699209496925 21.193443116062756 29.101715198481486 42-43 28.342432510322425 21.552525189951492 21.415162182617383 28.689880117108697 44-45 28.37631924500214 21.753656669779723 21.424749492665597 28.445274592552543 46-47 28.444843234020016 21.447115844285747 21.285606066177824 28.82243485551641 48-49 28.39373730618715 21.510130281864566 21.50285455986526 28.59327785208302 50-51 28.59341345862928 21.60030353629597 21.551894730472526 28.25438827460222 52-53 28.56203537443291 21.43909230488979 21.310189883849027 28.688682436828273 54-55 28.608162141078562 21.602296653548038 21.379360064773618 28.410181140599782 56-57 28.6128242354235 21.685252801765913 21.538686720440527 28.16323624237006 58-59 28.71553020074293 21.410877668226654 21.415980761338965 28.457611369691456 60-61 28.872666611520113 21.54391963309161 21.361706234317698 28.221707521070577 62-63 28.772936151943345 21.77808063940413 21.385192562583775 28.06379064606875 64-65 28.97946032144788 21.418117077806116 21.210044290559175 28.392378310186828 66-67 28.698151827102524 21.62934200228764 21.470411173318883 28.20209499729095 68-69 28.769122853898054 21.842808470839735 21.31137684548066 28.07669182978155 70-71 29.00234882259657 21.50145118338769 21.082552885910054 28.413647108105682 72-73 28.70528109028961 21.887580835176756 21.14851636197208 28.258621712561556 74-75 28.96154352372271 21.88251007843386 21.07428549144247 28.081660906400963 76-77 29.247654773592725 21.484714174644882 20.86442803716042 28.40320301460198 78-79 28.994421302683023 21.779126654598553 21.026776401235523 28.199675641482898 80-81 29.425774877650895 21.588091353996738 20.774877650897228 28.211256117455143 82-83 29.387559222349292 21.379268093448783 20.491953929096553 28.741218755105375 84-85 30.132750439835792 20.820227115210322 20.028522684864317 29.01849976008957 86-87 30.375990684746274 20.59685234571611 19.71979115802126 29.307365811516355 88-89 30.046371648803856 20.425022264533364 19.68491846574333 29.843687620919447 90-91 25.27342595329589 22.70913390481821 22.30268992018918 29.71475022169672 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 3.0 14 7.0 15 10.0 16 10.0 17 12.5 18 20.5 19 26.5 20 39.0 21 65.0 22 87.0 23 124.0 24 186.0 25 256.0 26 322.5 27 399.5 28 527.5 29 672.0 30 836.5 31 1049.5 32 1296.0 33 1558.5 34 1896.5 35 2257.5 36 2706.5 37 3311.5 38 4010.5 39 4853.5 40 5805.5 41 6822.0 42 8011.5 43 9334.0 44 10633.0 45 12054.5 46 13768.5 47 15607.0 48 17322.0 49 19115.5 50 20967.0 51 22755.0 52 24526.5 53 26138.0 54 27625.0 55 28947.0 56 30120.0 57 31106.5 58 31826.0 59 32196.0 60 32467.5 61 32668.0 62 32282.5 63 31421.0 64 30188.5 65 28585.5 66 26462.0 67 24224.0 68 21849.5 69 19156.0 70 16583.0 71 14086.5 72 11642.0 73 9295.0 74 7149.0 75 5566.0 76 4350.5 77 3235.5 78 2404.0 79 1758.0 80 1269.5 81 883.0 82 595.0 83 393.0 84 267.5 85 202.0 86 144.5 87 104.5 88 70.5 89 37.5 90 23.0 91 16.5 92 12.5 93 12.5 94 11.0 95 6.0 96 2.5 97 1.0 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.021969823531969527 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06729289577495852 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 8.475438180153914E-4 74-75 0.0 76-77 0.0 78-79 6.729384530490841E-4 80-81 8.156540321857082E-4 82-83 0.0010210645619122497 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.014777597162701344 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 3922.0 32-33 8861.0 34-35 10270.0 36-37 11752.0 38-39 13000.0 40-41 14183.0 42-43 15077.0 44-45 15897.0 46-47 16688.0 48-49 17272.0 50-51 17497.0 52-53 17638.0 54-55 17703.0 56-57 17659.0 58-59 17599.0 60-61 17283.0 62-63 17261.0 64-65 17118.0 66-67 16601.0 68-69 16028.0 70-71 15783.0 72-73 15570.0 74-75 14634.0 76-77 14090.0 78-79 13120.0 80-81 12507.0 82-83 11670.0 84-85 10967.0 86-87 10604.0 88-89 9697.0 90-91 9012.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.80310693958381 #Duplication Level Percentage of deduplicated Percentage of total 1 99.81322592699264 99.61670061176487 2 0.17870128412850672 0.3566988674023661 3 0.0067744735961188145 0.020283405383184985 4 7.093791062563983E-4 0.002831929552096548 5 3.152458555219096E-4 0.0015731257915456863 6 0.0 0.0 7 2.736903209556149E-4 0.0019120601059463573 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0013731139707480954 0.0 0.0 0.0 0.0 2 0.0013731139707480954 0.0 0.0 0.0 0.0 3 0.0013731139707480954 0.0 0.0 2.288523284580159E-4 0.0 4 0.0013731139707480954 0.0 0.0 2.288523284580159E-4 0.0 5 0.0013731139707480954 0.0 0.0 2.288523284580159E-4 0.0 6 0.0013731139707480954 0.0 0.0 2.288523284580159E-4 0.0 7 0.0018308186276641272 0.0 0.0 2.288523284580159E-4 0.0 8 0.006636717525282461 0.0 0.0 2.288523284580159E-4 0.0 9 0.006865569853740477 0.0 0.0 2.288523284580159E-4 0.0 10-11 0.006865569853740477 0.0 0.0 2.288523284580159E-4 0.0 12-13 0.006865569853740477 0.0 0.0 2.288523284580159E-4 1.1442616422900795E-4 14-15 0.006865569853740477 0.0 0.0 2.288523284580159E-4 2.288523284580159E-4 16-17 0.006865569853740477 0.0 0.0 2.288523284580159E-4 2.288523284580159E-4 18-19 0.006865569853740477 0.0 1.1442616422900795E-4 2.288523284580159E-4 4.577046569160318E-4 20-21 0.006865569853740477 0.0 2.288523284580159E-4 2.288523284580159E-4 4.577046569160318E-4 22-23 0.007094422182198493 0.0 2.288523284580159E-4 2.288523284580159E-4 4.577046569160318E-4 24-25 0.007323274510656509 0.0 2.288523284580159E-4 2.288523284580159E-4 5.721308211450398E-4 26-27 0.007323274510656509 0.0 2.288523284580159E-4 2.288523284580159E-4 6.865569853740477E-4 28-29 0.007323274510656509 0.0 2.288523284580159E-4 2.288523284580159E-4 6.865569853740477E-4 30-31 0.007323274510656509 0.0 2.288523284580159E-4 2.288523284580159E-4 6.865569853740477E-4 32-33 0.007666553003343533 0.0 2.288523284580159E-4 2.288523284580159E-4 8.009831496030556E-4 34-35 0.008009831496030557 0.0 2.288523284580159E-4 2.288523284580159E-4 9.154093138320636E-4 36-37 0.008009831496030557 0.0 2.288523284580159E-4 2.288523284580159E-4 9.154093138320636E-4 38-39 0.008124257660259565 0.0 2.288523284580159E-4 2.288523284580159E-4 9.154093138320636E-4 40-41 0.008467536152946589 0.0 2.288523284580159E-4 2.288523284580159E-4 9.154093138320636E-4 42-43 0.009268519302549644 0.0 2.288523284580159E-4 2.288523284580159E-4 9.154093138320636E-4 44-45 0.009382945466778652 0.0 2.288523284580159E-4 2.288523284580159E-4 9.154093138320636E-4 46-47 0.009382945466778652 0.0 2.288523284580159E-4 2.288523284580159E-4 9.154093138320636E-4 48-49 0.009382945466778652 0.0 2.288523284580159E-4 2.288523284580159E-4 9.154093138320636E-4 50-51 0.009840650123694684 0.0 2.288523284580159E-4 2.288523284580159E-4 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