##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-36A_S47_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446424
Sequences flagged as poor quality	0
Sequence length	61
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.38752844829131	40.0	40.0	40.0	40.0	40.0
2	39.284447072738025	40.0	40.0	40.0	40.0	40.0
3	39.225265666720425	40.0	40.0	40.0	40.0	40.0
4	39.10516459688547	40.0	40.0	40.0	40.0	40.0
5	39.09631202623515	40.0	40.0	40.0	40.0	40.0
6	39.256222783721306	40.0	40.0	40.0	40.0	40.0
7	39.26101643280827	40.0	40.0	40.0	40.0	40.0
8	39.23183341397416	40.0	40.0	40.0	40.0	40.0
9	39.26521871583965	40.0	40.0	40.0	40.0	40.0
10	39.27442969016003	40.0	40.0	40.0	40.0	40.0
11	39.25457412683906	40.0	40.0	40.0	40.0	40.0
12	39.27760156264	40.0	40.0	40.0	40.0	40.0
13	39.124957439564184	40.0	40.0	40.0	40.0	40.0
14	39.278685733741916	40.0	40.0	40.0	40.0	40.0
15	39.22478182176585	40.0	40.0	40.0	40.0	40.0
16	39.24029174058742	40.0	40.0	40.0	40.0	40.0
17	39.28683941723563	40.0	40.0	40.0	40.0	40.0
18	39.21615325340932	40.0	40.0	40.0	40.0	40.0
19	39.27752092181424	40.0	40.0	40.0	40.0	40.0
20	38.86789240721825	40.0	40.0	40.0	40.0	40.0
21	39.10842607028296	40.0	40.0	40.0	40.0	40.0
22	39.102306327616795	40.0	40.0	40.0	40.0	40.0
23	39.23117036718456	40.0	40.0	40.0	40.0	40.0
24	39.252522265828	40.0	40.0	40.0	40.0	40.0
25	38.9635593068473	40.0	40.0	40.0	40.0	40.0
26	39.19660233320789	40.0	40.0	40.0	40.0	40.0
27	39.19886923642098	40.0	40.0	40.0	40.0	40.0
28	38.87009658978908	40.0	40.0	40.0	40.0	40.0
29	39.133684568929986	40.0	40.0	40.0	40.0	40.0
30	38.701700625414404	40.0	40.0	40.0	40.0	40.0
31	38.89167249072631	40.0	40.0	40.0	40.0	40.0
32	39.008745049549304	40.0	40.0	40.0	40.0	40.0
33	39.00255810619501	40.0	40.0	40.0	40.0	40.0
34	39.0364586133362	40.0	40.0	40.0	40.0	40.0
35	38.709253982760785	40.0	40.0	40.0	40.0	40.0
36	38.80978621221081	40.0	40.0	40.0	40.0	40.0
37	38.99539451283981	40.0	40.0	40.0	40.0	40.0
38	38.887747970539216	40.0	40.0	40.0	40.0	40.0
39	38.740918947010016	40.0	40.0	40.0	40.0	40.0
40	38.46566492840887	40.0	40.0	40.0	40.0	40.0
41	38.52496281561923	40.0	40.0	40.0	40.0	40.0
42	38.318495421393116	40.0	40.0	40.0	40.0	40.0
43	38.74970879701808	40.0	40.0	40.0	40.0	40.0
44	38.22288228231457	40.0	40.0	40.0	40.0	40.0
45	38.59044316613802	40.0	40.0	40.0	40.0	40.0
46	38.24946687454079	40.0	40.0	40.0	40.0	40.0
47	37.65102234646883	40.0	40.0	40.0	24.0	40.0
48	38.06492482483021	40.0	40.0	40.0	40.0	40.0
49	37.71467483827034	40.0	40.0	40.0	24.0	40.0
50	38.03720230095156	40.0	40.0	40.0	40.0	40.0
51	37.59651810834543	40.0	40.0	40.0	24.0	40.0
52	37.7133756249664	40.0	40.0	40.0	24.0	40.0
53	36.982294858699355	40.0	40.0	40.0	24.0	40.0
54	36.32497356772933	40.0	40.0	40.0	24.0	40.0
55	36.30149812734082	40.0	40.0	40.0	24.0	40.0
56	36.077872157410894	40.0	40.0	40.0	24.0	40.0
57	36.21850975753992	40.0	40.0	40.0	24.0	40.0
58	36.26368654015017	40.0	40.0	40.0	24.0	40.0
59	35.80935612780675	40.0	40.0	40.0	24.0	40.0
60	35.16440872354533	40.0	40.0	40.0	12.0	40.0
61	35.082226762002044	40.0	40.0	40.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.02330302311438004
1101	2	-0.011345680021385363
1101	3	0.08013538556719624
1101	4	-0.0035258705793310696
1101	5	0.15364836740609888
1101	6	0.18050221229268004
1101	7	0.16555573168876236
1101	8	0.09724228058178852
1101	9	0.19771753693619587
1101	10	0.2161401695729026
1101	11	0.17108944618463084
1101	12	0.27640417032281306
1101	13	0.13246362806986411
1101	14	0.22895034003936843
1101	15	0.29727644028200473
1101	16	0.20810122977817258
1101	17	0.13211426445383978
1101	18	0.29189521257087137
1101	19	0.25838061006428603
1101	20	0.2894858992371283
1101	21	0.2832255048992849
1101	22	0.36022605979276534
1101	23	0.17693031137004311
1101	24	0.32030697587585166
1101	25	0.3645479604343578
1101	26	0.2606328926006114
1101	27	0.22255585268033684
1101	28	0.24413407921447572
1101	29	0.23449496769359257
1101	30	0.2563111062787371
1101	31	0.2180824111858044
1101	32	0.21047374111717687
1101	33	0.25059895362286966
1101	34	0.26505694679956093
1101	35	0.31236498610631713
1101	36	0.23252610834096998
1101	37	0.11859382404751528
1101	38	0.19379433856302342
1101	39	0.31360047981039685
1101	40	0.1922169930314226
1101	41	0.3655213361835692
1101	42	0.255691338678659
1101	43	0.41568660010715064
1101	44	0.30928052983756515
1101	45	0.2042211465491377
1101	46	0.26320009771662
1101	47	0.13917235615662094
1101	48	0.3545147246239466
1101	49	0.4365141926630045
1101	50	0.2939293458172614
1101	51	0.20961113817817534
1101	52	0.136710411764561
1101	53	0.05819599875812287
1101	54	0.20793815910682412
1101	55	-0.0882989039268196
1101	56	0.6018667020999544
1101	57	0.20137208824734643
1101	58	0.4540532789182947
1101	59	0.18108823764953286
1101	60	-0.08385401093676137
1101	61	0.45503475060053233
1102	1	-0.1096922441545658
1102	2	-0.044671550313729824
1102	3	-0.04223328671904625
1102	4	-0.0941213727886776
1102	5	-0.10931430478903081
1102	6	-0.08069065304014345
1102	7	0.012993727871950966
1102	8	-0.13975288588763846
1102	9	-0.029953674431666855
1102	10	0.033342188277025286
1102	11	-0.16860491805635291
1102	12	0.01303442316482517
1102	13	-0.09702003610879473
1102	14	-0.13824872252514098
1102	15	0.10353201938976042
1102	16	0.06712535478508386
1102	17	-0.07351480690155654
1102	18	0.00870285317408559
1102	19	-0.07772955605137355
1102	20	0.04854404408021651
1102	21	-0.12103676380702666
1102	22	-0.018324578878768705
1102	23	-0.003776079997642512
1102	24	0.001224173920725491
1102	25	-0.040290028576556836
1102	26	-0.02571720543661371
1102	27	0.03653616382130309
1102	28	0.10616862049429443
1102	29	-0.13938206271394193
1102	30	0.2093094556866859
1102	31	0.010214427534705806
1102	32	-0.2286748519057511
1102	33	-0.08196772186375512
1102	34	-0.13150191284778145
1102	35	-0.10877344748861617
1102	36	0.010362039684864044
1102	37	-0.019627510363598333
1102	38	0.1475389920695349
1102	39	-0.13527720482475303
1102	40	0.20423733514884646
1102	41	0.06370432801008263
1102	42	-0.06522136215710361
1102	43	-0.09366303948612398
1102	44	-0.29440000936229893
1102	45	-0.27181813036941804
1102	46	0.05614626399123068
1102	47	-0.2103712837332381
1102	48	0.13830751959095267
1102	49	0.3580650319643439
1102	50	-0.31156364619341304
1102	51	-0.08682278822460887
1102	52	-0.12848535736965516
1102	53	-0.08775975994721819
1102	54	-0.03659564798422821
1102	55	-0.21773517294909794
1102	56	-0.08075342620620063
1102	57	0.10707801923263105
1102	58	-0.1300081855241899
1102	59	-0.22718856557139588
1102	60	-0.144256181615539
1102	61	-0.2583272912099446
1103	1	0.03044506099512745
1103	2	-0.0683971653435762
1103	3	0.05932998344925977
1103	4	-0.022031555776415246
1103	5	-0.03138535245830809
1103	6	-0.18473254485393653
1103	7	-0.02188179957572345
1103	8	-0.037536937919270486
1103	9	-7.405153982702473E-4
1103	10	-0.14905988941396942
1103	11	-0.13027563724998714
1103	12	-0.05252667335330585
1103	13	0.13365300387889079
1103	14	-0.04449621852968022
1103	15	-0.01782525906036625
1103	16	-0.14044949555793806
1103	17	0.047537500076508366
1103	18	-0.09269919003765636
1103	19	-0.13450086969656638
1103	20	0.06266402898704371
1103	21	-0.16553928880570368
1103	22	0.06449557196555844
1103	23	-0.1050693435760337
1103	24	0.04146292579098798
1103	25	-0.07603385059753975
1103	26	-0.08236419950083729
1103	27	-0.06770774731156592
1103	28	-0.26078004765934537
1103	29	-0.10873922849944506
1103	30	-0.0488110167859972
1103	31	-0.12283471900651222
1103	32	-0.09689026860215932
1103	33	-0.08724353408096874
1103	34	-0.10884078422994747
1103	35	-0.28451083014379464
1103	36	-0.16963134612493036
1103	37	-0.22516662756831352
1103	38	-0.17227895744408528
1103	39	-0.10253647768146124
1103	40	-0.36374969287987824
1103	41	0.026921271991973583
1103	42	0.016530417288308286
1103	43	-0.2864827095633231
1103	44	0.04220108358163799
1103	45	0.023815775659542737
1103	46	0.14892363627350136
1103	47	-0.2351147653322414
1103	48	0.20111416039331687
1103	49	0.12690636861351834
1103	50	0.15144520817737117
1103	51	-0.15911656704939503
1103	52	-0.17906982936837323
1103	53	-0.21766400958870946
1103	54	-0.24678315202097423
1103	55	0.10958662877185787
1103	56	0.533733010090458
1103	57	0.3301986446766776
1103	58	0.14428160244779775
1103	59	0.10321014577844068
1103	60	0.1615622175853133
1103	61	0.2901182526857937
1104	1	-0.014653809493488268
1104	2	-0.005538077217991599
1104	3	0.12827684605524325
1104	4	0.04564103112466711
1104	5	-0.05340934322369151
1104	6	-0.02478569147480414
1104	7	0.11362265868955745
1104	8	0.08107171229544718
1104	9	-0.0802681398404701
1104	10	0.09483764599890065
1104	11	-0.04002350645607322
1104	12	-0.0764135153397163
1104	13	0.042742367804549986
1104	14	-0.07116276864673665
1104	15	0.10353201938976042
1104	16	0.187321022150563
1104	17	-0.1070577838407587
1104	18	-0.013659131452051554
1104	19	0.06762334401850723
1104	20	-0.26731898876394666
1104	21	-0.12383201188529114
1104	22	-0.12454400585291836
1104	23	-0.1183812512065856
1104	24	0.05153863932953584
1104	25	0.0044339406757174515
1104	26	-0.07882691892368143
1104	27	1.9793880382934503E-4
1104	28	0.0670351473985562
1104	29	-0.08068185307033104
1104	30	0.016437338286266368
1104	31	-0.07923351096983566
1104	32	0.0983691732514842
1104	33	-0.009291271828814729
1104	34	0.0306224756917004
1104	35	-0.32680279759343733
1104	36	-0.0958573872892785
1104	37	0.01391546657560383
1104	38	-0.06210461380048571
1104	39	-0.14645819713781805
1104	40	-0.04733499189517687
1104	41	-0.157120270173003
1104	42	0.021431328269173378
1104	43	0.09920907791429556
1104	44	0.04662025618626586
1104	45	0.3207744622231701
1104	46	-0.04168741874811133
1104	47	-0.11533284907216057
1104	48	-0.2642082036794875
1104	49	-0.030474450914759643
1104	50	0.13288079825102983
1104	51	-0.16229448633782084
1104	52	0.1901729235527725
1104	53	0.07436462859226367
1104	54	0.03608080205071218
1104	55	0.08694686758200021
1104	56	-0.13106789161500387
1104	57	0.3111311289461227
1104	58	0.048887691484893026
1104	59	-0.03711169624924793
1104	60	-0.13587043738073845
1104	61	0.06592148586901914
1201	1	-0.018313689392577714
1201	2	0.15378514110746266
1201	3	0.13888931333544008
1201	4	0.07359943404309632
1201	5	0.07199780360974017
1201	6	0.19064976316756344
1201	7	0.17196161805185994
1201	8	0.06554979447100351
1201	9	0.09199494511848627
1201	10	0.08535038451291399
1201	11	0.14677842179133194
1201	12	0.1072378366838791
1201	13	-0.03126064064041856
1201	14	0.05034169072148842
1201	15	0.13314862032071773
1201	16	0.029034177582936138
1201	17	-0.008270637244216061
1201	18	0.07127021746833861
1201	19	0.11731006303685376
1201	20	-0.016143444815782004
1201	21	0.015314489909115991
1201	22	0.26313084520536734
1201	23	0.044459715759742835
1201	24	0.04142955358884848
1201	25	-0.11095295245342385
1201	26	0.005243720311646882
1201	27	0.09639711207368151
1201	28	0.1831978403121326
1201	29	0.08718700715541416
1201	30	0.045426192826766965
1201	31	0.0949483459750553
1201	32	-0.077595246556875
1201	33	0.06226589454767151
1201	34	0.17913187412115406
1201	35	-0.1912865650193183
1201	36	0.03800656940806846
1201	37	-0.09549599157266186
1201	38	-0.05768077838848029
1201	39	-0.1160954071012199
1201	40	-0.11214684564210842
1201	41	-0.30406030262631134
1201	42	-0.10305788752160794
1201	43	0.0025598223267664366
1201	44	0.09917921104774763
1201	45	-0.07207041335275477
1201	46	-0.001286608599940564
1201	47	0.4349401613863293
1201	48	0.15316615821038226
1201	49	0.043937184897579584
1201	50	-0.15496461031711561
1201	51	0.1579213162892401
1201	52	0.03816946502549001
1201	53	0.27452689511206074
1201	54	0.009034408039177322
1201	55	-0.04976563641544374
1201	56	0.4697683139541695
1201	57	0.1844033458384189
1201	58	0.08357364062559469
1201	59	0.27672596616532275
1201	60	0.534319900062556
1201	61	0.37354090573660415
1202	1	-0.2177272024968886
1202	2	-0.27987649739029763
1202	3	-0.18468251935642144
1202	4	-0.09170460681973935
1202	5	-0.14120930754386762
1202	6	-0.20354752273683374
1202	7	-0.208055594063552
1202	8	-0.08863808851848631
1202	9	-0.19202089640255338
1202	10	-0.08864338406381478
1202	11	-0.10064175303914169
1202	12	-0.1091049075329309
1202	13	-0.17946014180780878
1202	14	-0.007944428823023486
1202	15	-0.03802028949895231
1202	16	-0.15051368564449774
1202	17	-0.09471769006787412
1202	18	-0.16751999393787287
1202	19	-0.3896106417036478
1202	20	-0.07946570183410273
1202	21	-0.020145655278263064
1202	22	-0.05758027015564693
1202	23	-0.05041808618937438
1202	24	-0.22274852805713863
1202	25	-0.06703018385278625
1202	26	-0.044432357238491704
1202	27	0.057877035256645115
1202	28	-0.1286243330361856
1202	29	0.006489278995282177
1202	30	-0.10233902116922877
1202	31	-0.01367922687761336
1202	32	0.0367258709556566
1202	33	0.02687348222188035
1202	34	0.10122713043026721
1202	35	-0.141255886017305
1202	36	0.011343727655933833
1202	37	0.0047924707320134985
1202	38	-0.0785863269051319
1202	39	-0.006969798501536673
1202	40	-0.24240112277311
1202	41	-0.10130795787401325
1202	42	-0.032898595768287464
1202	43	-0.09444957261956688
1202	44	0.11279351622811618
1202	45	0.10490758542008649
1202	46	0.08016016532370429
1202	47	0.042703144182183905
1202	48	0.3954545727036205
1202	49	-0.009328734317037402
1202	50	-0.08144258119995129
1202	51	-0.12663240383857044
1202	52	0.13158901830734493
1202	53	0.01609415384362478
1202	54	-0.05916461501389847
1202	55	0.25165755053522787
1202	56	0.2123356284657376
1202	57	0.0032418467906651927
1202	58	0.35948942875720036
1202	59	0.19763788953953565
1202	60	0.1991019994625205
1202	61	0.03012732888370806
1203	1	-0.06637174674075652
1203	2	0.10469878789374576
1203	3	0.07374026953240076
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2403	14	-0.10005529945127023
2403	15	0.04090974674100778
2403	16	-0.05139565393216827
2403	17	-0.02083465269600282
2403	18	0.014741585102633792
2403	19	-0.1862773188412703
2403	20	-0.1113267146055179
2403	21	-0.05540135069083618
2403	22	-0.13960970819012175
2403	23	-0.20682688472092536
2403	24	-0.015099078776856345
2403	25	0.08299971071519963
2403	26	0.10190547965130037
2403	27	-0.01611908839691978
2403	28	-0.11624347938134605
2403	29	0.0736855664145395
2403	30	-0.0445351777015901
2403	31	0.014896001153545058
2403	32	0.15053883671669155
2403	33	0.05145090077620296
2403	34	-0.047165107243586135
2403	35	0.077792241619413
2403	36	0.005618211097697667
2403	37	0.10033781361904204
2403	38	-0.02893516667129603
2403	39	-0.04373749938413596
2403	40	0.18641996321120757
2403	41	-0.016641934123796887
2403	42	-0.023074748593920447
2403	43	-0.021347668267523545
2403	44	0.08579730775984018
2403	45	0.08387563121583952
2403	46	-0.10259145427079375
2403	47	0.18002339720226956
2403	48	-0.28558033430908836
2403	49	-0.05567526209699736
2403	50	0.23199070784125553
2403	51	-0.007889713871946924
2403	52	-0.09707772584436469
2403	53	-0.1406266790577817
2403	54	0.5052217924338791
2403	55	-0.21897404681897115
2403	56	0.01829422841569084
2403	57	-0.6273251657315484
2403	58	-0.12583710264570414
2403	59	0.23891438235698814
2403	60	-0.14482270182739398
2403	61	-0.024279737752380015
2404	1	-0.015654277894604718
2404	2	0.022462911123518836
2404	3	0.10462334767493786
2404	4	0.057416241033550364
2404	5	0.1908747231043293
2404	6	0.05979936425583787
2404	7	0.045615061193153394
2404	8	0.16210358934091573
2404	9	0.13763387792239
2404	10	0.07590150771597592
2404	11	0.04619261594062607
2404	12	-0.011510401609193366
2404	13	0.04207538887047235
2404	14	0.051585609002799515
2404	15	0.03686444320636184
2404	16	-0.0021434392412089665
2404	17	0.18016153550723857
2404	18	0.045944531474987116
2404	19	0.06844797251881829
2404	20	0.032700262673465375
2404	21	0.012177119662659663
2404	22	0.1529919920612599
2404	23	-0.045304613811197214
2404	24	0.13179439543352345
2404	25	0.05270502516830078
2404	26	0.032916306313587995
2404	27	0.07995042898484428
2404	28	0.0571699226190816
2404	29	-0.06459553386937245
2404	30	-0.056336126866355585
2404	31	0.0049695477349231965
2404	32	0.16913563815702304
2404	33	0.06665943842796196
2404	34	0.06542058571594112
2404	35	0.11488277944187075
2404	36	0.03209948949091057
2404	37	0.011192979835605854
2404	38	0.22368373256167473
2404	39	0.23299218190327764
2404	40	0.09722359689866522
2404	41	0.24127837404072494
2404	42	-0.0434172144576479
2404	43	0.042855421429671026
2404	44	0.06842440388572157
2404	45	0.21950280625090812
2404	46	0.08720929198464233
2404	47	-0.06168348899296916
2404	48	-0.054225044897577845
2404	49	0.334799594054779
2404	50	0.1847353786530732
2404	51	0.12444582086661882
2404	52	0.19020324077705197
2404	53	0.05800545437281812
2404	54	-0.0027858794713893076
2404	55	0.34474028904750753
2404	56	-0.15412717239949103
2404	57	-0.03350294973616741
2404	58	-0.005798517664537428
2404	59	0.4635833261172877
2404	60	0.3297596833879197
2404	61	0.25990927712280154
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	10.0
15	44.0
16	124.0
17	269.0
18	395.0
19	503.0
20	556.0
21	552.0
22	592.0
23	750.0
24	962.0
25	1070.0
26	1250.0
27	1579.0
28	2263.0
29	2967.0
30	3753.0
31	4296.0
32	4563.0
33	5445.0
34	6892.0
35	10658.0
36	15624.0
37	24306.0
38	50592.0
39	177371.0
40	129035.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.663163978235694	25.4868184373873	23.123791774095636	21.72622581028137
2	28.154848305646652	20.58715481246528	19.94874827518234	31.30924860670573
3	30.35679085353834	18.033528663333513	18.13298568177338	33.47669480135477
4	30.197973227245846	18.377820188878736	20.291919789258642	31.132286794616775
5	27.4568571582173	19.30026163467914	20.745300431876423	32.497580775227135
6	27.850877192982455	19.380230453559843	22.27232406859846	30.496568284859237
7	28.87658369621705	21.571420891349927	22.11865849506299	27.43333691737003
8	26.709809508449368	23.628209952869916	22.920362704514094	26.741617834166618
9	27.14235793774528	23.1441409960038	22.461382004551727	27.252119061699194
10	29.339148432879952	22.010689389459348	21.66908589143953	26.981076286221175
11	27.451481103166497	23.06126014730391	22.053921832159563	27.43333691737003
12	28.91018404028457	21.11557622350053	21.808863322760423	28.165376413454474
13	28.583588695948247	21.990753185312617	21.269689801623567	28.155968317115565
14	29.313836173682418	21.878976040714658	21.2625217282225	27.54466605738043
15	28.172320484561762	21.340474526459154	22.35878895399889	28.128416034980198
16	28.863367560883823	21.852319767754423	21.062711682167627	28.22160098919413
17	29.63191943085497	22.005313334408545	21.03538340232604	27.327383832410447
18	28.553124383993694	21.361530742074798	22.068033976667923	28.017310897263588
19	30.21678941992366	21.95872085730158	20.70856405569593	27.115925667078834
20	28.38937870723796	21.954240811425908	22.390821282009927	27.2655591993262
21	28.67766965933731	22.998091500456965	20.657939537300862	27.66629930290486
22	30.249941759403615	22.00195330000179	20.815861154418222	26.932243786176368
23	29.543886529398062	22.05952188950415	21.056887622529256	27.339703958568535
24	29.551726609680486	21.18120889557909	21.2625217282225	28.004542766517933
25	28.801094923212013	22.99786749816318	20.843637438847374	27.357400139777432
26	28.61875705607225	23.08881242943928	21.068535741805995	27.22389477268247
27	28.942888375176963	22.739144848843253	20.742388402057237	27.57557837392255
28	28.894951884307297	23.776947475942155	20.60977904413741	26.718321595613137
29	28.384450656774725	23.923220973782772	20.74955647545831	26.942771893984197
30	28.77981470530258	23.321326810386537	20.659283551063563	27.23957493324732
31	29.135754350124543	22.923050732039496	21.714334354783794	26.226860563052167
32	30.05640377757468	22.88138630539577	20.732084296543196	26.330125620486356
33	29.051594563900156	22.457176265559795	21.647406159628872	26.843823010911176
34	29.490573983477592	23.055212085371753	21.543196602333207	25.911017328817447
35	29.08535383402326	23.20327760156264	20.75403652133398	26.95733204308012
36	30.3926312205437	22.874442234288477	20.10106983495511	26.631856710212716
37	30.003315233947998	23.54734512481408	19.782762575488775	26.666577065749152
38	29.257835600236547	23.790611615862947	20.283183699801086	26.668369084099417
39	29.88840205723707	23.699442682293068	19.74669820618963	26.665457054280235
40	30.131444545992153	23.76507535437163	19.7294500295683	26.374030070067917
41	30.210965360285293	23.965781409601632	20.76299661308532	25.06025661702776
42	31.11055857211978	23.491120549074424	20.047533286740858	25.350787592064943
43	30.804347436517748	24.02200598534129	20.657939537300862	24.5157070408401
44	30.626937619841225	24.173431535938928	20.620083149651453	24.579547694568394
45	31.0912943748544	23.890516638890382	20.364720534738275	24.653468451516943
46	31.39795351504399	24.007669838539147	19.112771714782358	25.4816049316345
47	31.511746680286006	24.304696880096053	19.185796462555775	24.997759977062163
48	31.6026916115621	25.3391394727882	19.049603067935415	24.00856584771428
49	32.27716251814419	24.409529953586727	18.76780818235579	24.545499345913303
50	31.725892873143017	24.941087396734943	19.247621095640017	24.085398634482015
51	32.0802645019085	24.7354532910417	19.039074960127593	24.145207246922208
52	32.52154902066197	25.194657993297852	18.818432700750858	23.46536028528932
53	32.53633317205168	24.24802429976883	18.681791301542926	24.53385122663656
54	32.21645789652886	24.11944698313711	18.320027597082593	25.34406752325144
55	33.23029227819293	24.28386466677419	19.310341737899396	23.175501317133488
56	32.27156246079959	25.154337580416826	20.604850993674177	21.969248965109404
57	33.477142805942336	25.924233464150674	18.88854541870509	21.710078311201908
58	32.31703492643765	26.392846262745728	19.918507965521567	21.371610845295056
59	31.982599501818896	27.135637868931777	18.720095693779903	22.16166693546942
60	32.107592781750085	24.917343153593894	21.600093184954215	21.374970879701806
61	32.44135619948748	26.65112090747809	19.136067953335843	21.771454939698582
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	0.5
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	0.0
11	1.5
12	1.5
13	0.0
14	1.0
15	2.0
16	4.5
17	8.5
18	10.0
19	14.0
20	25.0
21	32.0
22	54.5
23	77.0
24	106.0
25	189.0
26	243.0
27	309.0
28	375.0
29	537.5
30	804.0
31	908.0
32	1083.0
33	1258.0
34	1592.5
35	2293.5
36	2660.0
37	3135.5
38	3611.0
39	4385.5
40	5907.0
41	6654.0
42	7918.0
43	10345.0
44	11508.0
45	13038.0
46	14568.0
47	16188.0
48	19428.0
49	21048.0
50	23217.0
51	25386.0
52	27589.0
53	31599.5
54	33407.0
55	34081.0
56	34755.0
57	35015.5
58	34675.5
59	34075.0
60	33173.0
61	30604.5
62	28938.0
63	26851.0
64	24764.0
65	22675.0
66	18195.5
67	15805.0
68	13736.0
69	11667.0
70	9837.5
71	6749.0
72	5490.0
73	4454.0
74	3418.0
75	2726.0
76	1602.5
77	1171.0
78	908.0
79	645.0
80	519.0
81	279.0
82	165.0
83	133.5
84	75.0
85	48.0
86	38.5
87	29.0
88	31.0
89	26.0
90	19.0
91	20.5
92	22.0
93	21.5
94	23.0
95	25.0
96	22.5
97	20.0
98	27.0
99	81.0
100	128.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011200114689174417
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	2.2400229378348834E-4
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	446424.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.94031610262114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.41120384345136	67.47858317848289
2	11.765692798287695	18.811064029229026
3	2.445575607446985	5.865002613365149
4	0.6348595966203462	2.0300350733845205
5	0.2357860699313702	0.9424406481454232
6	0.12291116884885663	0.5895334614192141
7	0.07862081675488292	0.4399481060542114
8	0.05069487514484766	0.3242051475089626
9	0.03825929290736973	0.2752613971990116
>10	0.21163700949287942	2.6773239231553276
>50	0.0020820279871300667	0.11127567499888422
>100	0.002379460556720076	0.30363042088370756
>500	2.974325695900095E-4	0.15169632617369258
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCGCCCCTGTTTCCACCCCCCTTGTCTC	638	0.14291346343386557	Illumina Single End PCR Primer 1 (96% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1708599896062937	0.0	0.0	0.0	2.2400229378348834E-4
2	1.1726520079565614	0.0	0.0	0.0	2.2400229378348834E-4
3	1.172876010250345	0.0	0.0	0.0	2.2400229378348834E-4
4	1.1735480171316954	0.0	0.0	0.0	4.480045875669767E-4
5	1.1742200240130458	0.0	0.0	0.0	4.480045875669767E-4
6	1.1780280630073652	0.0	0.0	0.0	4.480045875669767E-4
7	1.181164095120334	0.0	0.0	0.0	4.480045875669767E-4
8	1.1950522373349104	0.0	0.0	0.0	4.480045875669767E-4
9	1.1984122717416625	0.0	0.0	0.0	4.480045875669767E-4
10	1.2019963084421985	0.0	0.0	0.0	4.480045875669767E-4
11	1.2084923749619196	0.0	0.0	0.0	4.480045875669767E-4
12	1.2199164919448775	0.0	0.0	0.0	4.480045875669767E-4
13	1.2405247029729585	0.0	0.0	0.0	4.480045875669767E-4
14	1.2653889575829256	0.0	0.0	0.0	4.480045875669767E-4
15	1.308621400283139	0.0	0.0	0.0	4.480045875669767E-4
16	1.3520778452771356	0.0	0.0	0.0	4.480045875669767E-4
17	1.3919502535705965	0.0	0.0	0.0	4.480045875669767E-4
18	1.4488468361916027	0.0	0.0	0.0	4.480045875669767E-4
19	1.5147035105639481	0.0	0.0	0.0	4.480045875669767E-4
20	1.5996003799078902	0.0	0.0	0.0	4.480045875669767E-4
21	1.9188036485493611	0.0	0.0	0.0	4.480045875669767E-4
22	2.061045105101876	0.0	0.0	0.0	4.480045875669767E-4
23	2.2368869057219145	0.0	0.0	0.0	4.480045875669767E-4
24	2.4747773417199794	0.0	0.0	0.0	4.480045875669767E-4
25	2.7574682364747414	0.0	0.0	0.0	4.480045875669767E-4
26	3.102655771195097	0.0	0.0	0.0	4.480045875669767E-4
27	3.501379854129706	0.0	0.0	0.0	4.480045875669767E-4
28	4.023753203232801	0.0	0.0	0.0	8.960091751339534E-4
29	4.5898069996236766	0.0	0.0	0.0	8.960091751339534E-4
30	5.222389477268247	0.0	0.0	0.0	8.960091751339534E-4
31	5.9654050857480785	0.0	0.0	0.0	8.960091751339534E-4
32	6.7680053043743165	0.0	0.0	0.0	8.960091751339534E-4
33	7.633326165259932	0.0	0.0	0.0	8.960091751339534E-4
34	8.561367668404925	0.0	0.0	0.0	8.960091751339534E-4
35	9.568705983549272	0.0	0.0	0.0	8.960091751339534E-4
36	10.655341110692973	0.0	0.0	0.0	8.960091751339534E-4
37	11.827545114061968	0.0	0.0	0.0	8.960091751339534E-4
38	13.017669300933642	0.0	0.0	0.0	8.960091751339534E-4
39	14.295602386968442	0.0	0.0	0.0	8.960091751339534E-4
40	15.664032399691774	0.0	0.0	0.0	0.0011200114689174417
41	17.04903858215508	0.0	0.0	0.0	0.0011200114689174417
42	18.485117287601025	0.0	0.0	0.0	0.0011200114689174417
43	19.9668924609788	0.0	0.0	0.0	0.0011200114689174417
44	21.484059996774366	0.0	0.0	0.0	0.0011200114689174417
45	22.995627475225348	0.0	0.0	0.0	0.0015680160564844185
46	24.609115997347814	0.0	0.0	0.0	0.0015680160564844185
47	26.202444313029766	0.0	0.0	0.0	0.0015680160564844185
48	27.790396573660914	0.0	0.0	0.0	0.0015680160564844185
49	29.483853914664085	0.0	0.0	0.0	0.0015680160564844185
50	31.148414959769188	0.0	0.0	0.0	0.0015680160564844185
>>END_MODULE