##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-36A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 260649 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 57 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.568292991724505 40.0 40.0 40.0 40.0 40.0 2 39.3434849164969 40.0 40.0 40.0 40.0 40.0 3 39.33923398900437 40.0 40.0 40.0 40.0 40.0 4 39.23972852380021 40.0 40.0 40.0 40.0 40.0 5 39.275869080641016 40.0 40.0 40.0 40.0 40.0 6 39.35890795667737 40.0 40.0 40.0 40.0 40.0 7 39.38519618337304 40.0 40.0 40.0 40.0 40.0 8 39.414308130857975 40.0 40.0 40.0 40.0 40.0 9 39.41977141673285 40.0 40.0 40.0 40.0 40.0 10-11 39.43263929652521 40.0 40.0 40.0 40.0 40.0 12-13 39.3458329017184 40.0 40.0 40.0 40.0 40.0 14-15 39.37710100556687 40.0 40.0 40.0 40.0 40.0 16-17 39.483473943886224 40.0 40.0 40.0 40.0 40.0 18-19 39.519476383949296 40.0 40.0 40.0 40.0 40.0 20-21 39.503531569275154 40.0 40.0 40.0 40.0 40.0 22-23 39.54056988517125 40.0 40.0 40.0 40.0 40.0 24-25 39.519783310122044 40.0 40.0 40.0 40.0 40.0 26-27 39.48749467674919 40.0 40.0 40.0 40.0 40.0 28-29 39.51075968064332 40.0 40.0 40.0 40.0 40.0 30-31 39.491778848031785 40.0 40.0 40.0 40.0 40.0 32-33 39.49120489019896 40.0 40.0 40.0 40.0 40.0 34-35 39.4840523849346 40.0 40.0 40.0 40.0 40.0 36-37 39.46665631566364 40.0 40.0 40.0 40.0 40.0 38-39 39.420993500952136 40.0 40.0 40.0 40.0 40.0 40-41 39.45097344696768 40.0 40.0 40.0 40.0 40.0 42-43 39.43954411483777 40.0 40.0 40.0 40.0 40.0 44-45 39.341240617134076 40.0 40.0 40.0 40.0 40.0 46-47 39.31568395256689 40.0 40.0 40.0 40.0 40.0 48-49 39.40613212632664 40.0 40.0 40.0 40.0 40.0 50-51 39.37104797285313 40.0 40.0 40.0 40.0 40.0 52-53 39.332630416613256 40.0 40.0 40.0 40.0 40.0 54-55 39.23335668117776 40.0 40.0 40.0 40.0 40.0 56-57 39.2544810589055 40.0 40.0 40.0 40.0 40.0 58-59 39.204872959268805 40.0 40.0 40.0 40.0 40.0 60-61 39.11255049145807 40.0 40.0 40.0 40.0 40.0 62-63 39.369749049545675 40.0 40.0 40.0 40.0 40.0 64-65 39.39162897522472 40.0 40.0 40.0 40.0 40.0 66-67 39.39723166315272 40.0 40.0 40.0 40.0 40.0 68-69 39.383171461806334 40.0 40.0 40.0 40.0 40.0 70-71 39.40329352067605 40.0 40.0 40.0 40.0 40.0 72-73 39.419091351573314 40.0 40.0 40.0 40.0 40.0 74-75 39.397744742541406 40.0 40.0 40.0 40.0 40.0 76-77 39.40418312959001 40.0 40.0 40.0 40.0 40.0 78-79 39.40250860122725 40.0 40.0 40.0 40.0 40.0 80-81 39.413478827247104 40.0 40.0 40.0 40.0 40.0 82-83 39.37206272607639 40.0 40.0 40.0 40.0 40.0 84-85 39.39710465504156 40.0 40.0 40.0 40.0 40.0 86-87 39.368710984580616 40.0 40.0 40.0 40.0 40.0 88-89 39.38640295017382 40.0 40.0 40.0 40.0 40.0 90-91 39.4391051045403 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11481126674298991 1101 2 -0.06294958630423508 1101 3 -0.03161253877964754 1101 4 -0.02853719173271685 1101 5 -0.017792712492315843 1101 6 -0.04756756286050745 1101 7 -0.029880907787493527 1101 8 -0.12035338472021095 1101 9 -0.15449986407131178 1101 10-11 0.055099183424019316 1101 12-13 0.09242848132191739 1101 14-15 -0.14338956509568135 1101 16-17 -0.15664829021810078 1101 18-19 0.003145720937361318 1101 20-21 -0.047202579888256935 1101 22-23 -0.03763662097469478 1101 24-25 0.048084526579849296 1101 26-27 -0.00737438891459874 1101 28-29 0.15497754604401592 1101 30-31 0.03302460766055759 1101 32-33 0.06469035576524362 1101 34-35 -0.04137268991204479 1101 36-37 -0.05870775074982504 1101 38-39 0.08178278026351649 1101 40-41 0.060930944884205473 1101 42-43 0.010238422696765781 1101 44-45 0.051409374753148995 1101 46-47 0.1714705437441424 1101 48-49 -0.008526146460681616 1101 50-51 0.059579391738715515 1101 52-53 -0.007291318055202112 1101 54-55 -0.08495570823093601 1101 56-57 -0.04006843050576947 1101 58-59 -0.07634208701558265 1101 60-61 0.26839058723012954 1101 62-63 -0.08878082636012863 1101 64-65 -0.13792046575332506 1101 66-67 -0.06498544126450412 1101 68-69 -0.45330676748859844 1101 70-71 -0.2497765411857742 1101 72-73 -0.5841253688782828 1101 74-75 0.030299098545839342 1101 76-77 0.036322214205384284 1101 78-79 0.19815481597083107 1101 80-81 -0.503768915943418 1101 82-83 0.2679608333725696 1101 84-85 -0.24311375445521577 1101 86-87 0.1209526980641158 1101 88-89 -0.07961529625414698 1101 90-91 -2.665169936058092 1102 1 0.11435084076674684 1102 2 0.10243377998518355 1102 3 0.2026702860981544 1102 4 0.12531908136566017 1102 5 -0.16805601859748265 1102 6 0.07776696538785899 1102 7 -0.22589904528219762 1102 8 -0.02377903620241284 1102 9 -0.07520793344764343 1102 10-11 -0.15222816711225562 1102 12-13 0.08016552875812977 1102 14-15 -0.1932800083790127 1102 16-17 -0.028497763811436982 1102 18-19 0.09714250617741982 1102 20-21 -0.0766780642604985 1102 22-23 -0.13304757171322024 1102 24-25 0.04459433324205264 1102 26-27 -0.065478502738479 1102 28-29 -0.11810850017123897 1102 30-31 -0.08242309493714828 1102 32-33 -0.038205379159215624 1102 34-35 -0.010391545332886665 1102 36-37 -0.10836511873031895 1102 38-39 -0.06155828025726606 1102 40-41 -0.1596786770838321 1102 42-43 0.1058003872278519 1102 44-45 -0.0580438147584843 1102 46-47 0.20013964042110644 1102 48-49 -0.18791398759422862 1102 50-51 -0.3222993769718485 1102 52-53 -0.10578533237175947 1102 54-55 -0.33374470488828933 1102 56-57 -0.08817935008980982 1102 58-59 -0.32573866569561716 1102 60-61 -0.2155182198068175 1102 62-63 -0.13245242952674374 1102 64-65 -0.33868616311130495 1102 66-67 -0.2526284846010327 1102 68-69 -0.27954730262868566 1102 70-71 -0.11107200229678682 1102 72-73 -0.008770713440732436 1102 74-75 -0.3771615353861151 1102 76-77 -0.11904671070830375 1102 78-79 -0.2027819129475361 1102 80-81 -0.6936653962746817 1102 82-83 -0.4657307623879987 1102 84-85 -0.1872693891768833 1102 86-87 0.11131843036532985 1102 88-89 -0.1567525839177506 1102 90-91 -1.2043640752522364 1103 1 0.0576459804062992 1103 2 0.19087150723110824 1103 3 0.09791605563884787 1103 4 -0.20882529472757483 1103 5 -0.08974780924625492 1103 6 -0.013952930054450974 1103 7 -0.09017058082591944 1103 8 0.06321610933325417 1103 9 -0.03467413890744098 1103 10-11 -0.15280598594442552 1103 12-13 -0.18495111596190128 1103 14-15 -0.07933963771019137 1103 16-17 -0.07482546963483827 1103 18-19 -0.1290519475479357 1103 20-21 -0.06169408202744364 1103 22-23 -0.04853272334153047 1103 24-25 -0.12473016902794853 1103 26-27 -0.02661527284912779 1103 28-29 -0.021856461099680757 1103 30-31 -0.014995174888412066 1103 32-33 -0.1256534276640835 1103 34-35 0.0677629858228741 1103 36-37 -0.009945348029482659 1103 38-39 0.03492730955300516 1103 40-41 -0.009482400362024634 1103 42-43 0.07205479479770105 1103 44-45 -0.04010832235490369 1103 46-47 -0.012551626036540142 1103 48-49 0.009752261449342825 1103 50-51 0.23252028086427146 1103 52-53 0.1864114030239037 1103 54-55 0.10120366195835118 1103 56-57 0.15955053876972158 1103 58-59 0.04261725083038215 1103 60-61 -0.36871484718886904 1103 62-63 0.0015380493921171023 1103 64-65 0.10533972233444899 1103 66-67 0.2062949199235149 1103 68-69 0.0387572200260351 1103 70-71 -0.13817524348127108 1103 72-73 0.07135842241684287 1103 74-75 0.16201141037545597 1103 76-77 -0.009831066770026098 1103 78-79 0.03385488021098837 1103 80-81 -0.011488525526381466 1103 82-83 -0.04306812934178339 1103 84-85 0.48585923334130143 1103 86-87 -0.06276685036131369 1103 88-89 0.21668100004215063 1103 90-91 0.5538776829895227 1104 1 -0.08268929029582495 1104 2 -0.05548331158441755 1104 3 -0.10163915937171453 1104 4 -0.035800196146027474 1104 5 -0.004595778746711687 1104 6 0.15224714843702003 1104 7 -0.049168132639628936 1104 8 0.027080132207906615 1104 9 -0.11401780072134216 1104 10-11 0.013831402158508865 1104 12-13 0.12128895580173094 1104 14-15 0.21551543339933943 1104 16-17 0.08314421770748481 1104 18-19 0.06173838678834187 1104 20-21 0.16160293342790766 1104 22-23 0.04633356083495954 1104 24-25 -0.11406654234832558 1104 26-27 -0.05450964575835826 1104 28-29 -0.01700867848367693 1104 30-31 -0.09201399415245959 1104 32-33 -0.01709035381559687 1104 34-35 0.1629976551392005 1104 36-37 0.01553934284012115 1104 38-39 0.1137452150450784 1104 40-41 0.03400106353666388 1104 42-43 0.048812560273297834 1104 44-45 -0.0722052000249036 1104 46-47 0.07938166093040877 1104 48-49 -0.026302614189788187 1104 50-51 0.014050592808025897 1104 52-53 -0.01625482472122286 1104 54-55 -0.046088043286587776 1104 56-57 0.016751473237519576 1104 58-59 -0.24102350363365588 1104 60-61 -0.25039021201732226 1104 62-63 -0.15838496490157894 1104 64-65 0.015409330268411736 1104 66-67 -0.2810842571126244 1104 68-69 -0.07299839565739319 1104 70-71 0.16596526082113172 1104 72-73 -0.01740969057723163 1104 74-75 -0.20253361732021347 1104 76-77 -0.2990912985504366 1104 78-79 0.09599697567622201 1104 80-81 0.24917003989408926 1104 82-83 -0.3416990811487679 1104 84-85 -0.1874027987442588 1104 86-87 0.18559916271058086 1104 88-89 -0.941537125917911 1104 90-91 0.02054434965619123 1201 1 -0.15152944554141357 1201 2 0.09697707395975641 1201 3 -0.1356057445357095 1201 4 -0.11954513687078361 1201 5 -0.0543514596845327 1201 6 -0.06501353036561142 1201 7 -0.10330001580485515 1201 8 -0.019931919354185368 1201 9 0.12473086242068376 1201 10-11 -0.03607544293224407 1201 12-13 0.057988966761129745 1201 14-15 -0.004099627085217605 1201 16-17 0.06537109242641037 1201 18-19 0.07187394324247975 1201 20-21 0.056766814265195364 1201 22-23 0.0011921656654152457 1201 24-25 0.059696651447900706 1201 26-27 0.10061122887742613 1201 28-29 0.036117963064548064 1201 30-31 0.10949160081074893 1201 32-33 -0.03436787344161729 1201 34-35 -0.009525412041007542 1201 36-37 -0.07594711017173239 1201 38-39 0.004106749033546464 1201 40-41 0.03235424284653732 1201 42-43 0.05051291761011356 1201 44-45 -0.040659784515561626 1201 46-47 -0.015907208339491774 1201 48-49 3.137333317582147E-4 1201 50-51 -0.05123898971543639 1201 52-53 0.1086390842078302 1201 54-55 -1.4011971050820193E-4 1201 56-57 0.019188362397059677 1201 58-59 -0.13303824978279266 1201 60-61 -0.11469995854199055 1201 62-63 -0.03007487589248825 1201 64-65 0.16209558020229053 1201 66-67 -0.09224629505364135 1201 68-69 0.14215059573630384 1201 70-71 0.23031560067727952 1201 72-73 -0.008979832147375078 1201 74-75 -0.7004451044156994 1201 76-77 -0.3563614439655325 1201 78-79 -0.2783885694452266 1201 80-81 0.10482248353499557 1201 82-83 -0.4255848903101409 1201 84-85 -0.5375463394141136 1201 86-87 -1.0586585403706579 1201 88-89 0.5976333809945302 1201 90-91 -0.06150693239509053 1202 1 0.13394490744089182 1202 2 0.16004301257714815 1202 3 0.0751789603497457 1202 4 0.1849436137039433 1202 5 0.07375633416656768 1202 6 0.12238696133363902 1202 7 -0.06367528796837263 1202 8 -0.025266894218248126 1202 9 0.07422339383585808 1202 10-11 -0.04679099073612747 1202 12-13 4.745938676649075E-4 1202 14-15 0.1364222428918751 1202 16-17 -0.004314166855643009 1202 18-19 -0.10520618397615067 1202 20-21 -0.10086953348092464 1202 22-23 -0.024544148262137355 1202 24-25 0.06373968083205739 1202 26-27 0.07967031258409918 1202 28-29 0.08717260855851094 1202 30-31 0.00624615776845161 1202 32-33 -0.00371579158604618 1202 34-35 -0.17050780504966667 1202 36-37 -0.0231846506175728 1202 38-39 -0.0676542441604866 1202 40-41 -0.08380671817169372 1202 42-43 -0.01063254827842286 1202 44-45 0.04155609509898994 1202 46-47 -0.34050821501836026 1202 48-49 -0.03220515468526486 1202 50-51 -0.16756377814869694 1202 52-53 0.08023476364721205 1202 54-55 0.0321462823111176 1202 56-57 -0.04041568261077089 1202 58-59 0.26474052602406317 1202 60-61 0.06538010443516384 1202 62-63 -0.2107203754935867 1202 64-65 -0.03841399598388762 1202 66-67 -0.21791790171477743 1202 68-69 -0.10053712816034022 1202 70-71 -0.030791489057342858 1202 72-73 -0.12296964961704049 1202 74-75 -0.13032296067376592 1202 76-77 -0.11709128417363246 1202 78-79 -0.018187348352974197 1202 80-81 -0.1569126710882145 1202 82-83 0.07931810355336921 1202 84-85 -0.5771806167450748 1202 86-87 -0.4616735645621475 1202 88-89 0.5976333809945302 1202 90-91 0.5538776829895227 1203 1 0.06253500530144152 1203 2 0.2128073162783295 1203 3 0.016228298001990993 1203 4 -0.023258358648519106 1203 5 -0.13577952670525661 1203 6 0.010823409657177763 1203 7 0.006061845865708904 1203 8 0.010164068939396032 1203 9 0.0271191035817111 1203 10-11 0.08920074636525044 1203 12-13 -0.03834877225408917 1203 14-15 -0.18972431540536405 1203 16-17 -0.1766453533494854 1203 18-19 -0.0645189790313907 1203 20-21 -0.19924200923209412 1203 22-23 -0.1406717617373019 1203 24-25 -0.12371852249220439 1203 26-27 0.03652803174288266 1203 28-29 -0.13216913548270526 1203 30-31 -0.11490697083102219 1203 32-33 -0.04402265918953674 1203 34-35 -0.12003635416930791 1203 36-37 -0.04598119517513766 1203 38-39 -0.08244379353794073 1203 40-41 -0.13404231996218385 1203 42-43 -0.2626100698992033 1203 44-45 0.055971289446695494 1203 46-47 -0.16295414934829466 1203 48-49 -0.01341415975508653 1203 50-51 -0.006100347930718897 1203 52-53 -0.04366436420684039 1203 54-55 -0.02665653815537894 1203 56-57 -0.14083620567122068 1203 58-59 -0.06187685421262046 1203 60-61 -0.05126161355994441 1203 62-63 -0.13722347376430122 1203 64-65 0.0011605709289170818 1203 66-67 0.19235270187949283 1203 68-69 0.03404975716311043 1203 70-71 -0.12853458370198467 1203 72-73 0.3280846060446976 1203 74-75 -0.14837098020164774 1203 76-77 0.13373576160218903 1203 78-79 0.15565446008736927 1203 80-81 0.06654052393124488 1203 82-83 -0.3448953027393813 1203 84-85 0.020755709414252976 1203 86-87 0.21350613945476482 1203 88-89 0.5976333809945302 1203 90-91 0.5538776829895227 1204 1 0.05628153187091556 1204 2 -0.11512953956803074 1204 3 0.009226900838910979 1204 4 -0.19389577318697349 1204 5 -0.3646372636473174 1204 6 -0.21205786264419402 1204 7 -0.31696502918082103 1204 8 0.03504638348633904 1204 9 -0.15878842049640696 1204 10-11 -0.24933169569229108 1204 12-13 -0.013785017815450828 1204 14-15 -0.1602644306063965 1204 16-17 -0.10207112451598732 1204 18-19 -0.10549896687762583 1204 20-21 -0.10358048627239214 1204 22-23 -0.16334493118530702 1204 24-25 -0.1542968302801384 1204 26-27 -0.07270692191874417 1204 28-29 -0.2576714303273988 1204 30-31 -0.048016273305577784 1204 32-33 -0.13410962060631704 1204 34-35 -0.16655389854734182 1204 36-37 -0.09623293709812941 1204 38-39 0.03714287679083128 1204 40-41 0.05465981390699426 1204 42-43 0.09469866076172195 1204 44-45 -0.2503604807790012 1204 46-47 0.032247215511368665 1204 48-49 0.029146933574438094 1204 50-51 -0.07929098582193461 1204 52-53 0.06780464275195897 1204 54-55 0.1196434500314183 1204 56-57 -0.07809018471945706 1204 58-59 -0.27990689586751216 1204 60-61 -0.1336805665812122 1204 62-63 -0.1890871136080463 1204 64-65 -0.1128169283171303 1204 66-67 -0.19056018898022842 1204 68-69 -0.12217027353302967 1204 70-71 -0.3736857035942549 1204 72-73 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0.01449588405757396 2401 46-47 -0.11610741260268753 2401 48-49 -0.0317914029253501 2401 50-51 -0.16088728124946527 2401 52-53 -0.02333709379700366 2401 54-55 -0.14820384815740795 2401 56-57 -0.09395378441381297 2401 58-59 0.034813571748863126 2401 60-61 -0.07551081357907208 2401 62-63 -0.05205523418700864 2401 64-65 0.14463181605725595 2401 66-67 0.2680349975251062 2401 68-69 -0.1574305497560431 2401 70-71 -0.029448668662524824 2401 72-73 0.033982265181144555 2401 74-75 -0.11564815359446357 2401 76-77 0.15912662515410148 2401 78-79 -0.0429650923046907 2401 80-81 0.1939973786608178 2401 82-83 0.2590439473792685 2401 84-85 0.20531467331593234 2401 86-87 -0.18357079776372842 2401 88-89 0.06136964473079587 2401 90-91 0.5538776829895227 2402 1 0.056956059804186054 2402 2 9.52330574371274E-4 2402 3 0.04474655469091715 2402 4 0.13842713923818195 2402 5 0.282849228656616 2402 6 0.011539921284764887 2402 7 0.2659367845041487 2402 8 0.10530696788527649 2402 9 0.11700576074047575 2402 10-11 0.07943212219065288 2402 12-13 0.14647020600580163 2402 14-15 0.06209123481129808 2402 16-17 0.1623931810287189 2402 18-19 0.16272459800249806 2402 20-21 0.13092490789458822 2402 22-23 0.02467013327186862 2402 24-25 0.06035386159084766 2402 26-27 0.09369034487212957 2402 28-29 0.14839712748526068 2402 30-31 0.052622745941775406 2402 32-33 0.09836752143180405 2402 34-35 0.07831797566957732 2402 36-37 0.058914162825594474 2402 38-39 -0.37047154691556017 2402 40-41 0.058633516088534066 2402 42-43 -0.0033388830391132274 2402 44-45 0.15660730154879587 2402 46-47 0.19729973233020104 2402 48-49 0.19809966753194885 2402 50-51 0.258243522094439 2402 52-53 0.060983405643149524 2402 54-55 0.26661552616883455 2402 56-57 0.10241980225381297 2402 58-59 0.11864681266231258 2402 60-61 0.22610658857720978 2402 62-63 0.17617028357428666 2402 64-65 -7.28532025163986E-4 2402 66-67 0.17708934070535065 2402 68-69 0.17087038364855545 2402 70-71 0.1726560589185624 2402 72-73 -0.022994711843900006 2402 74-75 0.14594551095694897 2402 76-77 -0.044721666370051594 2402 78-79 -0.3453543731670621 2402 80-81 -0.1517773503272295 2402 82-83 -0.057355825277419115 2402 84-85 0.47447403410031797 2402 86-87 0.5855991627105794 2402 88-89 -0.23569995233879837 2402 90-91 0.5538776829895227 2403 1 -0.07929328704035044 2403 2 0.02106728593650331 2403 3 0.025189851864375612 2403 4 0.21861899946961216 2403 5 0.37238400332356036 2403 6 0.1049958617616511 2403 7 0.20420298610923737 2403 8 -0.07215928016642437 2403 9 0.08546225946989239 2403 10-11 0.12449426686291076 2403 12-13 0.1482901092717981 2403 14-15 0.2075026222698213 2403 16-17 0.01814295884420858 2403 18-19 -0.036218741598148085 2403 20-21 -0.16373417844697968 2403 22-23 0.1290320952839039 2403 24-25 0.10735872508693234 2403 26-27 0.10921869740191426 2403 28-29 0.21483587825007078 2403 30-31 0.11809906933523706 2403 32-33 0.15759366969238187 2403 34-35 0.09949281922236963 2403 36-37 0.13085879725144167 2403 38-39 0.14917937622589506 2403 40-41 -0.03482586798443066 2403 42-43 -0.05096964095238121 2403 44-45 0.11279406547285475 2403 46-47 0.16657552688455013 2403 48-49 0.11616573047889744 2403 50-51 0.21400675202919928 2403 52-53 0.19291651894544515 2403 54-55 0.05684169693028451 2403 56-57 0.15495254697465555 2403 58-59 0.10852496282873858 2403 60-61 0.1367702224103553 2403 62-63 0.018900080252656437 2403 64-65 0.007835195391891148 2403 66-67 -0.16810079516819343 2403 68-69 -0.06128552964223388 2403 70-71 -0.14353622459004356 2403 72-73 0.18781228622899704 2403 74-75 0.1520007233789613 2403 76-77 0.3103136324227691 2403 78-79 0.35186080392265495 2403 80-81 0.43513921111399867 2403 82-83 0.2539311066493397 2403 84-85 0.3528992013440444 2403 86-87 0.06394659924616519 2403 88-89 -0.511088243856129 2403 90-91 0.5538776829895227 2404 1 0.0328797489928192 2404 2 -0.07167674455612172 2404 3 -0.13650750098819486 2404 4 0.21685510773065175 2404 5 -0.012650859734691267 2404 6 -0.16260048645852265 2404 7 -0.015615472755847293 2404 8 -0.026773996437455594 2404 9 -0.006936851598531746 2404 10-11 0.06175386020727558 2404 12-13 0.12495565531418151 2404 14-15 0.14601746035416596 2404 16-17 0.09110214920043092 2404 18-19 0.1028492773855163 2404 20-21 -0.058232495327516176 2404 22-23 0.10163938050607157 2404 24-25 0.039243687909852554 2404 26-27 0.08224075914685613 2404 28-29 0.19279814668470152 2404 30-31 0.08094819110052498 2404 32-33 0.1077465337641641 2404 34-35 0.10285485110260595 2404 36-37 0.22103890924598346 2404 38-39 0.10239609239099678 2404 40-41 0.09995440532096467 2404 42-43 0.2310715472631486 2404 44-45 0.06803555858085275 2404 46-47 0.11386237651030484 2404 48-49 0.19521356373922316 2404 50-51 0.102004405305685 2404 52-53 0.21218003559361165 2404 54-55 0.25665899087792354 2404 56-57 0.3638375680192496 2404 58-59 0.10575381920752847 2404 60-61 0.3690922434963966 2404 62-63 0.0482734498279882 2404 64-65 0.024422973216672972 2404 66-67 0.25580159619180165 2404 68-69 0.13547936739334432 2404 70-71 0.05175646627788666 2404 72-73 0.03969520826733941 2404 74-75 0.08228610900793853 2404 76-77 -0.1205205036761896 2404 78-79 0.16924992593761345 2404 80-81 0.22861143196646339 2404 82-83 0.42544486886623645 2404 84-85 0.39297997637535786 2404 86-87 0.24517363079569066 2404 88-89 0.06141716477831238 2404 90-91 0.5538776829895227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 6.0 31 146.0 32 1038.0 33 1907.0 34 2481.0 35 3193.0 36 4380.0 37 6548.0 38 16346.0 39 112396.0 40 112208.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.64984327582304 11.990454596027607 23.387774363224107 32.97192776492525 2 31.28269086862252 16.31483009382735 24.754686570600764 27.64779246694936 3 28.365349569727872 18.44933224374542 19.837789517703886 33.347528668822825 4 28.23260400001535 19.513215090025284 19.234295930542608 33.019884979416766 5 28.660382353279694 20.29472585737908 19.464106902385968 31.580784886955254 6 28.63352631316445 22.671101749862842 21.888440009361247 26.806931927611462 7 27.787561049534048 21.794827526673803 21.37011843513691 29.047492988655243 8 26.95464014824534 22.654988125793693 21.909157526021588 28.48121419993938 9 26.58364313693895 22.80730023901876 22.680309535045215 27.92874708899708 10-11 27.14992192565481 21.557151571653833 22.11921012549444 29.173716377196918 12-13 27.43785704146189 21.320434760923696 21.86637969069515 29.375328506919267 14-15 27.540101822757805 21.445507176317577 21.84681314718261 29.167577853742007 16-17 28.124220695264512 21.132826137832872 21.123810181508464 29.619142985394152 18-19 28.06897398417028 21.36551454254572 21.26537987868743 29.30013159459656 20-21 27.946203515071993 21.593599054667386 21.339617646720303 29.120579783540318 22-23 28.123261550974682 20.879803874175614 21.313145264320983 29.68378931052872 24-25 28.056505108402487 21.190950281796592 21.490586957939605 29.261957651861316 26-27 28.086046752529263 21.478501739887744 21.547751957613496 28.887699549969497 28-29 28.422130911685827 21.147405131038294 21.308157714013866 29.122306243262013 30-31 28.173982769766713 21.356319766426942 21.568676349001425 28.90102111480492 32-33 28.157899351731153 21.390728605861504 21.65572579026642 28.795646252140923 34-35 28.29746260763712 21.14546851458277 21.440815797088433 29.116253080691674 36-37 28.270343762451066 21.264063289423422 21.568023396713123 28.89756955141239 38-39 28.4323490870785 21.54970468364727 21.543333241301386 28.47461298797284 40-41 28.531474388077594 21.105757999180135 21.640432500099177 28.722335112643087 42-43 28.285672257898437 21.44909282302809 21.811779740097357 28.45345517897611 44-45 28.261167822422056 21.468166380636262 21.779149226033017 28.491516570908658 46-47 28.52594384427361 21.197076963993972 21.62261426816449 28.654364923567925 48-49 28.549717991801966 21.393024176718004 21.62847694168263 28.428780889797405 50-51 28.458860543831356 21.609004793166896 21.82282319801913 28.10931146498262 52-53 28.63129083631563 21.190376708388587 21.646653465826173 28.531678989469604 54-55 28.586813022845448 21.341663179678807 21.827246512201214 28.24427728527453 56-57 28.680179126774423 21.587010167503777 21.867637460253192 27.8651732454686 58-59 28.694120487017393 21.39036828050271 21.731717988563844 28.183793243916057 60-61 28.669325717287116 21.621610531153568 21.6634659575865 28.04559779397282 62-63 28.715577799223468 21.52658392620688 21.709720133761248 28.048118140808402 64-65 28.712455470297886 21.510131279243847 21.58424451438538 28.193168736072888 66-67 28.759765625 21.5600860252809 21.720286165730336 27.959862183988765 68-69 28.73752727451028 21.69580033832651 21.832479344921424 27.734193042241778 70-71 29.0611770565286 21.36008964887524 21.459699510251514 28.119033784344648 72-73 28.681944126492947 21.6176319674137 21.555269618484516 28.145154287608836 74-75 28.828820593451194 21.908879828875442 21.415251293489586 27.847048284183778 76-77 29.23275583772732 21.51511890670397 21.231034111696975 28.02109114387173 78-79 29.087317469949518 21.489161168708765 21.562754186409823 27.860767174931894 80-81 29.372775448864985 21.586648738432334 21.04563790239658 27.9949379103061 82-83 29.268928456849537 21.352662816811037 20.67893808148861 28.699470644850816 84-85 29.61211224435872 21.473867779950325 20.556994134122498 28.35702584156846 86-87 30.612168580083022 21.083729105119478 20.27598070378819 28.02812161100931 88-89 30.101417399804497 20.014662756598238 19.953567937438905 29.930351906158357 90-91 25.05920663114269 21.80284191829485 23.44582593250444 29.69212551805802 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 1.5 12 2.5 13 1.0 14 0.5 15 2.5 16 5.0 17 5.5 18 8.5 19 16.5 20 28.0 21 37.5 22 48.5 23 77.5 24 114.5 25 152.0 26 185.0 27 231.0 28 305.5 29 411.0 30 533.0 31 655.5 32 823.5 33 982.5 34 1146.0 35 1409.0 36 1741.0 37 2110.5 38 2565.5 39 3134.0 40 3689.5 41 4350.5 42 5107.0 43 5869.0 44 6706.5 45 7634.5 46 8681.0 47 9821.5 48 10958.5 49 12135.0 50 13232.5 51 14142.0 52 15054.5 53 16024.5 54 17069.5 55 17893.0 56 18402.0 57 18865.0 58 19272.5 59 19522.0 60 19478.0 61 19270.5 62 19074.5 63 18600.5 64 17848.0 65 16940.5 66 15636.5 67 14298.5 68 12887.0 69 11207.0 70 9698.5 71 8338.0 72 6938.5 73 5529.5 74 4365.0 75 3462.0 76 2656.5 77 1964.0 78 1470.0 79 1095.0 80 781.0 81 559.0 82 398.0 83 266.5 84 173.5 85 122.5 86 77.5 87 42.5 88 32.0 89 25.0 90 16.5 91 10.0 92 6.5 93 7.0 94 8.0 95 5.5 96 3.0 97 2.5 98 3.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.02455409381965785 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06240117161383438 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.001578768718276616 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 2619.0 32-33 5826.0 34-35 6831.0 36-37 7747.0 38-39 8486.0 40-41 9180.0 42-43 9754.0 44-45 10191.0 46-47 10431.0 48-49 10786.0 50-51 10964.0 52-53 10921.0 54-55 10983.0 56-57 10644.0 58-59 10779.0 60-61 10502.0 62-63 10269.0 64-65 10177.0 66-67 9655.0 68-69 9362.0 70-71 9018.0 72-73 8733.0 74-75 8312.0 76-77 7933.0 78-79 7206.0 80-81 6825.0 82-83 6184.0 84-85 5654.0 86-87 5260.0 88-89 4872.0 90-91 4545.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.80895840815533 #Duplication Level Percentage of deduplicated Percentage of total 1 99.81727901847842 99.62658649970548 2 0.17532756541431296 0.34998523368480583 3 0.0065224259149947094 0.019529896106099443 4 4.491186936637163E-4 0.0017930427606482765 5 4.2187149861963455E-4 0.002105327742965663 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001534630863728616 0.0 0.0 0.0 0.0 2 0.001534630863728616 0.0 0.0 0.0 0.0 3 0.001534630863728616 0.0 0.0 0.0 0.0 4 0.001534630863728616 0.0 0.0 0.0 0.0 5 0.0019182885796607699 0.0 0.0 0.0 3.83657715932154E-4 6 0.002301946295592924 0.0 0.0 0.0 3.83657715932154E-4 7 0.003069261727457232 0.0 0.0 0.0 3.83657715932154E-4 8 0.009975100614236003 0.0 0.0 0.0 3.83657715932154E-4 9 0.010358758330168158 0.0 0.0 0.0 3.83657715932154E-4 10-11 0.010358758330168158 0.0 0.0 0.0 3.83657715932154E-4 12-13 0.010358758330168158 0.0 0.0 0.0 3.83657715932154E-4 14-15 0.010358758330168158 0.0 0.0 0.0 3.83657715932154E-4 16-17 0.010358758330168158 0.0 0.0 0.0 3.83657715932154E-4 18-19 0.010358758330168158 0.0 0.0 0.0 3.83657715932154E-4 20-21 0.010358758330168158 0.0 0.0 0.0 3.83657715932154E-4 22-23 0.010358758330168158 0.0 0.0 0.0 7.67315431864308E-4 24-25 0.010358758330168158 0.0 0.0 0.0 7.67315431864308E-4 26-27 0.010358758330168158 0.0 0.0 0.0 7.67315431864308E-4 28-29 0.010358758330168158 0.0 0.0 0.0 7.67315431864308E-4 30-31 0.010358758330168158 0.0 0.0 0.0 7.67315431864308E-4 32-33 0.011126073762032465 0.0 0.0 0.0 7.67315431864308E-4 34-35 0.011126073762032465 0.0 0.0 0.0 7.67315431864308E-4 36-37 0.011317902619998543 0.0 0.0 0.0 7.67315431864308E-4 38-39 0.011701560335930696 0.0 0.0 0.0 7.67315431864308E-4 40-41 0.011893389193896773 0.0 0.0 0.0 7.67315431864308E-4 42-43 0.012085218051862849 0.0 0.0 0.0 7.67315431864308E-4 44-45 0.012277046909828927 0.0 0.0 0.0 7.67315431864308E-4 46-47 0.012277046909828927 0.0 0.0 0.0 7.67315431864308E-4 48-49 0.012277046909828927 0.0 0.0 0.0 7.67315431864308E-4 50-51 0.012277046909828927 0.0 0.0 0.0 7.67315431864308E-4 52-53 0.012277046909828927 0.0 0.0 0.0 7.67315431864308E-4 54-55 0.012468875767795004 0.0 0.0 0.0 7.67315431864308E-4 56-57 0.01266070462576108 0.0 0.0 0.0 7.67315431864308E-4 58-59 0.01342802005762539 0.0 0.0 0.0 7.67315431864308E-4 60-61 0.014195335489489697 0.0 0.0 0.0 7.67315431864308E-4 62-63 0.014387164347455773 0.0 0.0 0.0 7.67315431864308E-4 64-65 0.014770822063387928 0.0 0.0 0.0 7.67315431864308E-4 66-67 0.015346308637286159 0.0 0.0 0.0 7.67315431864308E-4 68-69 0.015346308637286159 0.0 0.0 0.0 7.67315431864308E-4 70-71 0.015346308637286159 0.0 0.0 0.0 7.67315431864308E-4 72-73 0.015346308637286159 0.0 0.0 0.0 7.67315431864308E-4 74-75 0.015346308637286159 0.0 0.0 0.0 7.67315431864308E-4 76-77 0.015346308637286159 0.0 0.0 0.0 7.67315431864308E-4 78-79 0.015346308637286159 0.0 0.0 0.0 7.67315431864308E-4 80 0.015346308637286159 0.0 0.0 0.0 7.67315431864308E-4 >>END_MODULE