##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-35C_S46_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1061363
Sequences flagged as poor quality	0
Sequence length	61
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.467111629103336	40.0	40.0	40.0	40.0	40.0
2	39.18980782258285	40.0	40.0	40.0	40.0	40.0
3	39.155043090818126	40.0	40.0	40.0	40.0	40.0
4	39.02560952284939	40.0	40.0	40.0	40.0	40.0
5	39.053809111491546	40.0	40.0	40.0	40.0	40.0
6	39.18167111534885	40.0	40.0	40.0	40.0	40.0
7	39.2156425275801	40.0	40.0	40.0	40.0	40.0
8	39.21122933435592	40.0	40.0	40.0	40.0	40.0
9	39.22025546396473	40.0	40.0	40.0	40.0	40.0
10	39.24086669687939	40.0	40.0	40.0	40.0	40.0
11	39.227585661079196	40.0	40.0	40.0	40.0	40.0
12	39.23758035657923	40.0	40.0	40.0	40.0	40.0
13	39.057468556940464	40.0	40.0	40.0	40.0	40.0
14	39.23334052534336	40.0	40.0	40.0	40.0	40.0
15	39.13411339946842	40.0	40.0	40.0	40.0	40.0
16	39.32535051627012	40.0	40.0	40.0	40.0	40.0
17	39.280792716535245	40.0	40.0	40.0	40.0	40.0
18	39.366407157588874	40.0	40.0	40.0	40.0	40.0
19	39.317443702107575	40.0	40.0	40.0	40.0	40.0
20	39.330163195815196	40.0	40.0	40.0	40.0	40.0
21	39.3052895192314	40.0	40.0	40.0	40.0	40.0
22	39.3715929422827	40.0	40.0	40.0	40.0	40.0
23	39.36322634197725	40.0	40.0	40.0	40.0	40.0
24	39.35701922904793	40.0	40.0	40.0	40.0	40.0
25	39.314556848128305	40.0	40.0	40.0	40.0	40.0
26	39.28406775061878	40.0	40.0	40.0	40.0	40.0
27	39.30338630609886	40.0	40.0	40.0	40.0	40.0
28	39.316392223961074	40.0	40.0	40.0	40.0	40.0
29	39.309559500378285	40.0	40.0	40.0	40.0	40.0
30	39.318220062316094	40.0	40.0	40.0	40.0	40.0
31	39.26194148467584	40.0	40.0	40.0	40.0	40.0
32	39.27524513290929	40.0	40.0	40.0	40.0	40.0
33	39.28458406784484	40.0	40.0	40.0	40.0	40.0
34	39.25239150036321	40.0	40.0	40.0	40.0	40.0
35	39.28424111260709	40.0	40.0	40.0	40.0	40.0
36	39.258391332654334	40.0	40.0	40.0	40.0	40.0
37	39.256947905664695	40.0	40.0	40.0	40.0	40.0
38	39.254404006923174	40.0	40.0	40.0	40.0	40.0
39	38.01061088430631	40.0	40.0	40.0	40.0	40.0
40	39.24832879985453	40.0	40.0	40.0	40.0	40.0
41	39.25033753767561	40.0	40.0	40.0	40.0	40.0
42	39.22429555204016	40.0	40.0	40.0	40.0	40.0
43	39.24514421550403	40.0	40.0	40.0	40.0	40.0
44	39.20593802497355	40.0	40.0	40.0	40.0	40.0
45	39.06508517820953	40.0	40.0	40.0	40.0	40.0
46	39.059888087299065	40.0	40.0	40.0	40.0	40.0
47	39.15329628034895	40.0	40.0	40.0	40.0	40.0
48	39.222626000717945	40.0	40.0	40.0	40.0	40.0
49	39.201453225710715	40.0	40.0	40.0	40.0	40.0
50	39.190173390253854	40.0	40.0	40.0	40.0	40.0
51	39.15764540501223	40.0	40.0	40.0	40.0	40.0
52	39.16011016023735	40.0	40.0	40.0	40.0	40.0
53	39.08764484912325	40.0	40.0	40.0	40.0	40.0
54	38.997187578613534	40.0	40.0	40.0	40.0	40.0
55	39.04178306573717	40.0	40.0	40.0	40.0	40.0
56	39.05773613740068	40.0	40.0	40.0	40.0	40.0
57	38.986488128943634	40.0	40.0	40.0	40.0	40.0
58	38.98276838367269	40.0	40.0	40.0	40.0	40.0
59	38.95892734154102	40.0	40.0	40.0	40.0	40.0
60	38.925140597514705	40.0	40.0	40.0	40.0	40.0
61	38.81337487739821	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.056960137935945454
1101	2	-0.013820408739711354
1101	3	0.0496835255901118
1101	4	0.03725028609864012
1101	5	0.025968067694073227
1101	6	-0.027567785046983317
1101	7	-0.006284059856774604
1101	8	0.04288317030550104
1101	9	-0.07468603075419367
1101	10	0.1363474803386211
1101	11	-0.003818074378621361
1101	12	-0.03992680518020819
1101	13	-0.016864298138763445
1101	14	0.021295690162737912
1101	15	0.014036980805464339
1101	16	0.12786292964835155
1101	17	-0.015106645147817233
1101	18	-0.04111158303973639
1101	19	-0.03530870980574008
1101	20	0.05867950168528324
1101	21	0.10936805302431907
1101	22	0.056424723037459046
1101	23	-0.07250063382753069
1101	24	0.07793154797682433
1101	25	0.08034849033504798
1101	26	0.1223150368006003
1101	27	0.03124154942105406
1101	28	-0.059713935261797246
1101	29	0.013957744158140883
1101	30	-0.05824175392750419
1101	31	-0.02145820363735851
1101	32	0.09878026711448484
1101	33	0.09536027210646125
1101	34	0.04637039312376601
1101	35	0.0389612117845175
1101	36	0.10781691341581734
1101	37	0.0016404789035604495
1101	38	-0.08328741518556626
1101	39	1.247627385982888
1101	40	-0.2275496179786245
1101	41	-0.014657352037545479
1101	42	0.009103982858796655
1101	43	-0.03329469617265346
1101	44	0.03978971288266564
1101	45	0.03669291086822568
1101	46	0.0051003450362969716
1101	47	-0.10642802331395984
1101	48	-0.041231894191192
1101	49	-0.05774660313840485
1101	50	0.054829353552911186
1101	51	-0.03638672989063707
1101	52	0.03270821730944107
1101	53	0.06459401710999657
1101	54	-0.03886060263768343
1101	55	0.028574775029888144
1101	56	-0.03836594484250355
1101	57	0.050762056081588014
1101	58	0.017989615065701514
1101	59	-0.030513064970186576
1101	60	0.1268200484957731
1101	61	0.028447024138138488
1102	1	0.10068957338912554
1102	2	0.04013804925022413
1102	3	0.12238247666277147
1102	4	0.022819797799613184
1102	5	-0.09011250848514862
1102	6	-0.011218618135991676
1102	7	-0.0692904416391471
1102	8	-0.1235307299020576
1102	9	-0.054295605837793914
1102	10	-0.010873391888580386
1102	11	0.06588582388876318
1102	12	-0.1647771955825803
1102	13	-0.07339128178907117
1102	14	-0.04651102923493511
1102	15	-0.17014090806202375
1102	16	-0.03312610010816286
1102	17	-0.12293478799834645
1102	18	0.0025023779912913824
1102	19	0.009751629047542565
1102	20	-0.03342920864054122
1102	21	0.07348949119756298
1102	22	0.026997470969803317
1102	23	-0.06796418821000572
1102	24	0.0019119270346195094
1102	25	0.020578860073399596
1102	26	-0.014933075946821361
1102	27	-0.022944736482862993
1102	28	0.10462766720761607
1102	29	-0.011745891257547214
1102	30	-8.59635367170597E-4
1102	31	-0.053763300237953615
1102	32	-0.06638283357141006
1102	33	0.009460305899196442
1102	34	-0.013316705869193868
1102	35	-0.07347585162116843
1102	36	-0.09830076889115702
1102	37	-0.04320174159675361
1102	38	-0.12668984649432247
1102	39	1.1148619694973405
1102	40	-0.05619147059060481
1102	41	0.07084851002322523
1102	42	-0.030100450388054867
1102	43	-0.024316819927598488
1102	44	-0.060284305181625086
1102	45	0.0025149093004799283
1102	46	0.07521516579693355
1102	47	0.04192435954234952
1102	48	-0.04341654542115947
1102	49	0.0316742926344844
1102	50	-0.08112912142875928
1102	51	0.0394921024167445
1102	52	-0.013330359816812631
1102	53	0.0662592068252863
1102	54	0.023056227689991715
1102	55	0.09257326588668491
1102	56	-2.3788578895533874E-4
1102	57	-0.08540760185231022
1102	58	0.04041141885593191
1102	59	-0.01169108092889104
1102	60	0.149128805254108
1102	61	-0.03928618001179274
1103	1	0.044332682661291756
1103	2	0.11030814994483507
1103	3	-0.07833163207649818
1103	4	0.04340678661157682
1103	5	-0.04934303072742097
1103	6	0.04640844467607508
1103	7	-0.06887850350920388
1103	8	0.003073123579021342
1103	9	-0.04313856443199171
1103	10	-0.10563651772328342
1103	11	0.07835270811873585
1103	12	0.08446414579139372
1103	13	-0.07786911656008755
1103	14	0.0849379675646631
1103	15	0.05637689179165761
1103	16	-0.053996097145251554
1103	17	0.04064963337291516
1103	18	-0.0516333055027971
1103	19	0.029408126486032415
1103	20	-0.04476831040643958
1103	21	-0.050914761434917466
1103	22	-0.030692639982525804
1103	23	-0.0030954443432520407
1103	24	-0.08559889165433532
1103	25	-0.0335918895211762
1103	26	0.05943043448153418
1103	27	-0.020018311006033684
1103	28	-0.0626082332535347
1103	29	-0.032613763076426494
1103	30	-0.08840020711108565
1103	31	-0.005649068342286512
1103	32	-0.011121492831449586
1103	33	0.01061589330154078
1103	34	-0.043770728632757994
1103	35	-0.05511857091579486
1103	36	9.628607088103536E-4
1103	37	-0.05223462740045193
1103	38	-0.00720441141168493
1103	39	1.1974497202000336
1103	40	-0.04381809596192454
1103	41	-0.010731533044321395
1103	42	0.0644604255135448
1103	43	-0.017860407001947465
1103	44	-0.04676260431281776
1103	45	0.03382256137888362
1103	46	-0.002958152478043985
1103	47	0.017395799354666508
1103	48	0.03565078131467203
1103	49	-0.03093746949196685
1103	50	0.05977107495911582
1103	51	0.001855904708406797
1103	52	-9.814251975583943E-4
1103	53	0.044069803482067016
1103	54	0.004319241330883017
1103	55	0.03105882476328503
1103	56	-0.003463258627498078
1103	57	0.0648545349589682
1103	58	-0.016425771187627447
1103	59	0.06597926903610585
1103	60	-0.07914123701470999
1103	61	0.06322372040579438
1104	1	0.014580608524987326
1104	2	0.060800796350186204
1104	3	0.06445553516915226
1104	4	-0.06004235376993705
1104	5	0.12905331439671386
1104	6	0.11443747355335177
1104	7	-0.032687029953379465
1104	8	6.120257378015026E-4
1104	9	0.07693979028078957
1104	10	0.1069294409658923
1104	11	0.017987337350504617
1104	12	0.06048506602164849
1104	13	-0.041296634721554426
1104	14	-0.15524210557634177
1104	15	0.0438435164994857
1104	16	0.10139309877180835
1104	17	0.010732934903145974
1104	18	-0.04400595101314053
1104	19	-0.030163596840488083
1104	20	0.02871848239973218
1104	21	-0.015434982595849078
1104	22	0.03753444131340444
1104	23	0.029109101031643547
1104	24	0.07681954763688736
1104	25	0.11837362347817049
1104	26	0.09006385985292553
1104	27	0.053286604261408854
1104	28	0.016336682923295598
1104	29	-0.01362373515948434
1104	30	-0.059254870692903694
1104	31	-0.028089737310160956
1104	32	0.07523295611869685
1104	33	0.005657034557934537
1104	34	-0.0753408592727709
1104	35	0.11300600657163073
1104	36	-0.016813691771893957
1104	37	-0.06319688268912671
1104	38	-0.06489014373880764
1104	39	1.265098459678157
1104	40	0.05856314290268472
1104	41	-0.015210537016784542
1104	42	-0.12509299234191928
1104	43	0.08622422643977501
1104	44	-0.01746809955754003
1104	45	-6.083663574116827E-4
1104	46	-0.0751646136776074
1104	47	0.02047549649262237
1104	48	0.06783915180963618
1104	49	-0.012327371532400377
1104	50	0.046741310574098804
1104	51	0.06136760125831131
1104	52	-0.014887508632796198
1104	53	0.05497275087767406
1104	54	-0.05058692774136375
1104	55	0.0337420576221632
1104	56	0.06185414149059909
1104	57	0.05180895513579742
1104	58	0.1346975697302213
1104	59	-0.1344558891501748
1104	60	-0.06248147013247518
1104	61	0.08267850653928122
1201	1	-0.03179941743353254
1201	2	0.043275310694880886
1201	3	0.13076889857021
1201	4	0.03564934102845996
1201	5	-0.03573771279730664
1201	6	0.11890821572036714
1201	7	-0.004669320876303118
1201	8	-0.003266144686364214
1201	9	-0.13746501152556334
1201	10	0.016867354085178476
1201	11	-0.05588378482077161
1201	12	-0.044152169405428765
1201	13	-0.051821132140034365
1201	14	-0.07044403101124175
1201	15	-0.04172243228238415
1201	16	-0.1141231277736594
1201	17	0.0638132542111336
1201	18	0.112770896080157
1201	19	-0.028771758687355486
1201	20	-0.002328749712646072
1201	21	-0.006527359504282515
1201	22	-0.0383792897643076
1201	23	0.018164865295318577
1201	24	-0.04727696783844948
1201	25	-0.021535034056718416
1201	26	0.08118057452936966
1201	27	0.00981194344920766
1201	28	-0.07197484826709655
1201	29	0.045105415375694236
1201	30	0.03468185939922108
1201	31	0.12399909131041653
1201	32	-0.03590211064570781
1201	33	0.003931169356896191
1201	34	0.07627320651098302
1201	35	-0.04322835519994328
1201	36	-0.13326215183048618
1201	37	-0.008258780423055612
1201	38	-0.05954077287452009
1201	39	1.2143704385565073
1201	40	0.024898013483515058
1201	41	0.08756574096074132
1201	42	-0.04640475571109448
1201	43	-0.06550024110320862
1201	44	-0.008729429956936485
1201	45	-0.09631716813766644
1201	46	-0.006425233343634318
1201	47	-0.05488375674000423
1201	48	-0.01622949515772376
1201	49	0.03840206047436112
1201	50	-0.00890293968552669
1201	51	-0.03377508287476161
1201	52	-0.014721376912035566
1201	53	6.818921521585253E-4
1201	54	-0.0599908296675693
1201	55	-0.020363975154992886
1201	56	0.06333945078173997
1201	57	-0.013400743394207382
1201	58	0.020339688671541012
1201	59	-0.02012199313822549
1201	60	0.007761913720244706
1201	61	0.02340969245258151
1202	1	0.09805012974729976
1202	2	-7.942189574734471E-4
1202	3	0.012422418874038499
1202	4	0.02939751514472988
1202	5	-0.09190919979700851
1202	6	0.013299100908795936
1202	7	0.020666641729597757
1202	8	0.08306894149534827
1202	9	-0.02979629986912613
1202	10	0.07081126293706319
1202	11	0.00951206677139993
1202	12	-0.03199908731369305
1202	13	-0.01685180698665789
1202	14	0.08276222081463658
1202	15	0.017333951007124426
1202	16	-0.045447989006490275
1202	17	-8.157262153929423E-4
1202	18	0.060470282583224844
1202	19	-0.1334971457747045
1202	20	-0.05170064571031219
1202	21	-0.014977549396185452
1202	22	-0.07216544226211852
1202	23	-0.007667737684521114
1202	24	-0.11514141423050006
1202	25	-0.017905177206003486
1202	26	0.05724689062635946
1202	27	-0.05075627156685414
1202	28	-0.05643292597670779
1202	29	0.06402887432982851
1202	30	0.0415910034249265
1202	31	0.09695410153536699
1202	32	0.03909788935428793
1202	33	0.06082570631278372
1202	34	0.05289459126120022
1202	35	-0.06203495759550748
1202	36	-0.20357328241768613
1202	37	-0.1924758414806078
1202	38	-0.10459627973984453
1202	39	1.0583961469993923
1202	40	-0.19000850126962376
1202	41	-0.25217279248945346
1202	42	-0.08057069202426703
1202	43	-0.3092995399638596
1202	44	-0.04055823509862222
1202	45	-0.21352429638774595
1202	46	8.929086370841333E-4
1202	47	-0.08593108364622282
1202	48	-0.09746671397162743
1202	49	-0.10807033216367756
1202	50	-0.09833472466946347
1202	51	0.05329766033295158
1202	52	-0.07216367898624299
1202	53	-0.19138646893460987
1202	54	-0.10490756936957979
1202	55	-0.05437653723503644
1202	56	0.009914092908530847
1202	57	-0.21105630868780878
1202	58	-0.13992908141317884
1202	59	-0.19087863935499882
1202	60	-0.08237101061513386
1202	61	-0.03049039518983676
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2403	3	0.0945212108434248
2403	4	0.08751195562449965
2403	5	0.03034514581725034
2403	6	0.13455356116416084
2403	7	0.14557206282069757
2403	8	0.07881969741011119
2403	9	0.18500458286735721
2403	10	0.1149101122270011
2403	11	0.12980139225181375
2403	12	0.05659555608082911
2403	13	0.031473716421622555
2403	14	0.19904519804405396
2403	15	0.1125412364491396
2403	16	0.0850411702443239
2403	17	0.06943383313286944
2403	18	0.06992853172700109
2403	19	0.11518054076786655
2403	20	0.0017364378200639408
2403	21	0.08421458909026569
2403	22	0.08382742132930332
2403	23	0.004447318991175564
2403	24	0.03641842125951911
2403	25	0.1260636260841963
2403	26	0.12085729717236404
2403	27	0.07690125247194857
2403	28	0.06770619100020525
2403	29	0.08237467488297057
2403	30	0.12473947798176965
2403	31	0.06931950571689782
2403	32	0.10969208851454226
2403	33	0.0848322695158572
2403	34	0.10770772626542424
2403	35	-0.08141826011904385
2403	36	0.0709874988614203
2403	37	0.13421654778325376
2403	38	0.18434551124077103
2403	39	1.3202063299952513
2403	40	0.09677517097146193
2403	41	0.11227019505766123
2403	42	0.14239120413954964
2403	43	0.16163929900838525
2403	44	0.08971633033994664
2403	45	0.11660195212550661
2403	46	0.07232792862762238
2403	47	0.047013544701655974
2403	48	0.1132590358528276
2403	49	0.18031473296679934
2403	50	0.028617756593881438
2403	51	0.057710626241558316
2403	52	0.08153600431771224
2403	53	0.05273387556190556
2403	54	0.07897317522436964
2403	55	-0.005576328015031606
2403	56	0.11265938904082873
2403	57	0.028193580253621064
2403	58	0.08918975841886834
2403	59	-0.0021722208863153014
2403	60	-0.0819479132991674
2403	61	0.1004369080215497
2404	1	-0.06535327475779695
2404	2	-0.060831639164383944
2404	3	-0.01621504535664542
2404	4	0.07240874120680019
2404	5	-0.008496990537345539
2404	6	-0.04166457947861346
2404	7	0.03609147113748179
2404	8	-0.03563199034117304
2404	9	0.08425095231372381
2404	10	0.013143789167891384
2404	11	0.0909010168044162
2404	12	0.02223820082189576
2404	13	0.0663311117894807
2404	14	0.15070007392198193
2404	15	0.04497721170123725
2404	16	0.04145310425052884
2404	17	0.03907531494683525
2404	18	-0.02949413153373115
2404	19	-0.01150543691858985
2404	20	-0.015381033119510334
2404	21	-0.11544163333484647
2404	22	0.03887351877684608
2404	23	-0.03410584759721047
2404	24	0.02656377323739889
2404	25	-0.09911986647153981
2404	26	-0.06618292814243887
2404	27	0.04219075315982934
2404	28	0.005992783906627608
2404	29	0.06168644275010848
2404	30	-0.018086889303809528
2404	31	0.003867668194693863
2404	32	-0.04318070775481431
2404	33	-0.07455106380974996
2404	34	-0.013781247024809318
2404	35	7.374394989270172E-4
2404	36	0.06799653194873656
2404	37	0.07780994617805703
2404	38	-0.005669359202755686
2404	39	1.2292157229037102
2404	40	0.05010265875678499
2404	41	0.02552733224457171
2404	42	-0.05981715177920677
2404	43	-0.01883252110700795
2404	44	0.09729854089374612
2404	45	0.05223254564028679
2404	46	0.08606790313410784
2404	47	0.058791566584901034
2404	48	0.1075538304557
2404	49	0.04332191965229981
2404	50	0.10317492387408578
2404	51	0.05971412522399788
2404	52	-0.023893446393977058
2404	53	-0.00980674921929392
2404	54	0.03459276080871376
2404	55	0.007995977256122444
2404	56	-0.023204624532219498
2404	57	-0.013761936393436258
2404	58	0.02015603121430587
2404	59	0.04343789157169908
2404	60	-0.02085695969429935
2404	61	-0.0044943573293920736
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	4.0
17	25.0
18	69.0
19	105.0
20	187.0
21	222.0
22	343.0
23	532.0
24	805.0
25	1102.0
26	1401.0
27	1745.0
28	2541.0
29	2951.0
30	3832.0
31	4772.0
32	6309.0
33	7629.0
34	9731.0
35	12694.0
36	18504.0
37	26648.0
38	68141.0
39	474534.0
40	416537.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	30.909406112706023	14.841387913465987	22.771756693986884	31.477449279841107
2	31.320363844400063	17.45433330537237	23.6826851573464	27.542617692881173
3	27.655649355543833	20.16017185497852	19.74975503128062	32.43442375819703
4	27.976317222587582	20.413204919716364	20.327654466619304	31.282823391076747
5	28.20787986767958	20.870804804765193	19.480045940926903	31.441269386628324
6	29.291957605456382	23.185093130248557	21.9992594428108	25.523689821484265
7	28.344873525834235	23.050454933891608	21.44450108021478	27.160170460059373
8	26.61181895355312	24.785111220195162	21.577254907133565	27.02581491911815
9	25.968401008891394	24.836460287385183	22.64286582441634	26.552272879307083
10	27.15282141925053	22.642677387472524	22.220767070267193	27.98373412300975
11	26.588170117104138	23.560742177746917	22.049854762225554	27.80123294292339
12	27.740179373126818	22.909315662972986	21.896938182318397	27.453566781581795
13	26.669198002945272	21.959876121553133	22.087730587932686	29.28319528756891
14	26.868375852559396	23.017761124139433	21.99605601476592	28.11780700853525
15	26.979270993995456	22.502103427385354	21.949983182002764	28.56864239661643
16	27.188153346216136	23.1128275622949	21.509982918191042	28.18903617329792
17	27.29744677362976	22.302454485411683	21.611927304795813	28.788171436162745
18	28.25593128835281	22.70957250252741	21.765786069422056	27.268710139697728
19	27.13133960765544	22.262600071794473	22.605649528012563	28.000410792537522
20	27.123331037543235	22.410899946578127	21.694085812299846	28.771683203578796
21	26.997549377545667	22.623739474618958	22.85815503272679	27.52055611510859
22	28.08360570323254	21.92482685000325	21.83710945265663	28.154457994107577
23	27.27247887857406	23.114617713261158	21.62200868129	27.99089472687478
24	27.145755033857412	23.758224094866694	21.753726105017794	27.342294766258103
25	27.259194074034994	22.178462976380374	21.587524720571565	28.97481822901307
26	27.468735955559033	22.345889200961405	22.506531695565045	27.678843147914524
27	27.240350379653332	22.983842474252448	21.601280617470177	28.174526528624042
28	27.366037821178995	22.517461038306404	21.511490413741576	28.605010726773028
29	27.324204819651708	23.843680248887516	21.517426177471798	27.314688753988975
30	28.241327425207018	23.20431369851785	21.742702543804523	26.81165633247061
31	27.323922164235988	22.62901570904582	22.572484625900845	27.474577500817343
32	27.433309810121514	22.823576853536444	21.524304125921105	28.218809210420943
33	27.220710012898536	24.3773796097652	21.735206023209823	26.66670435412645
34	27.503596790165098	22.916758922253745	21.53711783810063	28.042526449480526
35	27.726706131643937	24.288862528654192	21.204997724623905	26.77943361507797
36	28.723066472074116	23.673333251677324	21.285460299633584	26.318139976614972
37	27.558808814703355	24.41247716379787	21.16128035365846	26.867433667840313
38	27.76919866247457	23.73297354439527	22.037606360877476	26.460221432252677
39	28.116090613476462	24.016617723301874	21.86888106616449	25.998410597057177
40	28.947589090631574	23.75464379293418	20.861194520630548	26.436572595803696
41	27.90110452314618	24.016194270951598	20.669271493353357	27.413429712548865
42	28.908582643261543	24.535809143525825	20.736260826880155	25.81934738633248
43	28.07088620952492	25.187235658299752	20.451626823245206	26.290251308930124
44	28.158038296040093	24.43640865566258	21.384201258193475	26.021351790103857
45	28.026038216896577	24.97995502010151	20.325845163247635	26.66816159975428
46	28.086809131277423	24.745727898937496	21.067344537165887	26.10011843261919
47	29.145542100110895	24.940760135787663	19.92136526334534	25.992332500756106
48	29.16240720658248	25.549128808899503	19.842598620830007	25.445865363688014
49	29.101636292201633	25.19675172396249	19.66217024712563	26.039441736710252
50	29.19491257939084	25.507955336675575	19.464499893062033	25.83263219087155
51	29.098244427212933	25.83131313226483	19.645870451485496	25.424571989036735
52	29.061876097056334	25.63496183680795	19.38855980470395	25.914602261431764
53	29.13847571471777	25.92468363792595	19.214067194729793	25.72277345262648
54	29.067811860786552	26.217231993201196	19.192679601606613	25.522276544405635
55	28.97180323791201	26.052255448889778	19.090735215001843	25.885206098196377
56	28.858835290093964	26.300426903896216	18.95411843073482	25.886619375275
57	28.676239891535694	26.66693675961947	18.952234061296654	25.70458928754818
58	28.612925078413326	26.437608998994687	18.876199754466665	26.073266168125325
59	28.55667665068407	26.736752647303515	18.777553014378682	25.92901768763373
60	28.4422012073155	27.016393071927325	18.77425536786189	25.767150352895285
61	28.65051824870473	26.68351921067533	18.5221267370353	26.143835803584636
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	2.5
2	3.5
3	5.0
4	3.0
5	1.0
6	2.0
7	2.0
8	1.0
9	4.5
10	8.0
11	10.5
12	14.5
13	16.0
14	28.0
15	40.0
16	43.5
17	66.0
18	85.0
19	111.0
20	182.5
21	228.0
22	318.0
23	408.0
24	550.0
25	859.0
26	1026.0
27	1316.5
28	1607.0
29	1944.0
30	2771.5
31	3262.0
32	3899.5
33	4537.0
34	5546.5
35	7763.5
36	8971.0
37	10471.0
38	11971.0
39	13878.0
40	18340.5
41	20896.0
42	23842.0
43	30084.5
44	33381.0
45	36893.0
46	40405.0
47	44628.0
48	52351.5
49	55852.0
50	59108.5
51	62365.0
52	66119.5
53	72546.0
54	75218.0
55	76495.5
56	77773.0
57	77444.5
58	75462.0
59	73808.0
60	71363.5
61	70029.5
62	71140.0
63	61402.5
64	51665.0
65	46776.5
66	37813.0
67	33738.0
68	29365.0
69	24992.0
70	21322.5
71	15037.5
72	12422.0
73	10299.5
74	8177.0
75	6489.0
76	3825.5
77	2850.0
78	2181.0
79	1512.0
80	1149.0
81	595.0
82	404.0
83	299.0
84	147.5
85	101.0
86	72.0
87	43.0
88	37.0
89	21.5
90	12.0
91	9.5
92	7.0
93	4.0
94	2.5
95	4.0
96	3.0
97	2.0
98	4.5
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03391864988698494
3	2.8265541572487454E-4
4	9.421847190829151E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	1.8843694381658302E-4
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.13785198843374
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1061363.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.60121295513798
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.23179315418352	66.25351691504157
2	13.091042886612358	20.84125785244149
3	2.5039365824345334	5.97949167373595
4	0.5817467126370477	1.8523097583429238
5	0.19577460694957635	0.7791948089500832
6	0.09802103605879998	0.4681556019239877
7	0.06486478696298031	0.3614321004230898
8	0.04817684101415705	0.30679479848589924
9	0.030070533029690004	0.2154285813033778
>10	0.15227394838742955	1.8095700009559907
>50	0.0017880424565675228	0.08621152529116742
>100	3.8315195497875485E-4	0.04605403414728637
>500	0.0	0.0
>1k	0.0	0.0
>5k	1.277173183262516E-4	1.000582348957158
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATTGCGATCTGGGGGGGGGGGGGGG	9842	0.927298200521405	TruSeq Adapter, Index 2 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.018407462856723	0.0	0.0	0.0	0.0
2	1.019349647575806	0.0	0.0	0.0	0.0
3	1.0205744877106135	0.0	0.0	0.0	0.0
4	1.0212340170139718	0.0	0.0	0.0	0.0
5	1.0231183864521376	0.0	0.0	0.0	0.0
6	1.025850722137478	0.0	0.0	0.0	0.0
7	1.0293368055980847	0.0	0.0	0.0	0.0
8	1.0657993542265936	0.0	0.0	0.0	9.421847190829151E-5
9	1.0690970007433838	0.0	0.0	0.0	9.421847190829151E-5
10	1.0724888657320821	0.0	0.0	0.0	9.421847190829151E-5
11	1.0768229154398636	0.0	0.0	0.0	9.421847190829151E-5
12	1.0868100734621426	0.0	0.0	0.0	9.421847190829151E-5
13	1.1014139366079276	0.0	9.421847190829151E-5	0.0	9.421847190829151E-5
14	1.1197865386300445	0.0	9.421847190829151E-5	0.0	9.421847190829151E-5
15	1.1440006859104754	0.0	9.421847190829151E-5	0.0	9.421847190829151E-5
16	1.177071369550286	0.0	9.421847190829151E-5	0.0	9.421847190829151E-5
17	1.2105189270777292	0.0	9.421847190829151E-5	0.0	9.421847190829151E-5
18	1.2442491400208977	0.0	9.421847190829151E-5	0.0	1.8843694381658302E-4
19	1.2964461734580912	0.0	9.421847190829151E-5	0.0	1.8843694381658302E-4
20	1.3548616260412318	0.0	2.8265541572487454E-4	0.0	1.8843694381658302E-4
21	1.4383391921519781	0.0	2.8265541572487454E-4	0.0	1.8843694381658302E-4
22	1.5338767226669858	0.0	3.7687388763316603E-4	0.0	1.8843694381658302E-4
23	1.6575855762825724	0.0	3.7687388763316603E-4	0.0	3.7687388763316603E-4
24	1.800986090526992	0.0	3.7687388763316603E-4	0.0	3.7687388763316603E-4
25	1.981885556590912	0.0	3.7687388763316603E-4	0.0	3.7687388763316603E-4
26	2.203016310159672	0.0	3.7687388763316603E-4	0.0	4.7109235954145753E-4
27	2.465791628311897	0.0	3.7687388763316603E-4	0.0	6.595293033580406E-4
28	2.7612607562162994	0.0	3.7687388763316603E-4	0.0	6.595293033580406E-4
29	3.104969741737747	0.0	3.7687388763316603E-4	0.0	7.537477752663321E-4
30	3.490040636426934	0.0	3.7687388763316603E-4	0.0	7.537477752663321E-4
31	3.9321137066206377	0.0	3.7687388763316603E-4	0.0	8.479662471746236E-4
32	4.413287442656283	0.0	3.7687388763316603E-4	0.0	9.421847190829151E-4
33	4.962204259993989	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
34	5.562752799937439	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
35	6.217571179700065	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
36	6.903387436720519	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
37	7.676732654143776	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
38	8.506891610127733	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
39	9.367577351009976	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
40	10.321633597553335	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
41	11.319030341174509	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
42	12.38162626735622	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
43	13.467871029986913	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
44	14.601413465515568	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
45	15.806185065806892	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
46	17.029706142007964	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
47	18.273295752725506	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
48	19.561073826768034	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
49	20.87749431627068	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
50	22.20785913961576	0.0	3.7687388763316603E-4	0.0	0.0010364031909912066
>>END_MODULE