##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-35C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 604074 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.569052798167114 40.0 40.0 40.0 40.0 40.0 2 39.33224737366614 40.0 40.0 40.0 40.0 40.0 3 39.308078149365805 40.0 40.0 40.0 40.0 40.0 4 39.18414962405268 40.0 40.0 40.0 40.0 40.0 5 39.218254717137306 40.0 40.0 40.0 40.0 40.0 6 39.3541850832845 40.0 40.0 40.0 40.0 40.0 7 39.38395627025828 40.0 40.0 40.0 40.0 40.0 8 39.38848551667511 40.0 40.0 40.0 40.0 40.0 9 39.396371967672835 40.0 40.0 40.0 40.0 40.0 10-11 39.403036714044966 40.0 40.0 40.0 40.0 40.0 12-13 39.31803057241331 40.0 40.0 40.0 40.0 40.0 14-15 39.351817823644126 40.0 40.0 40.0 40.0 40.0 16-17 39.460374060131706 40.0 40.0 40.0 40.0 40.0 18-19 39.50389190728288 40.0 40.0 40.0 40.0 40.0 20-21 39.48521869837139 40.0 40.0 40.0 40.0 40.0 22-23 39.53116671136318 40.0 40.0 40.0 40.0 40.0 24-25 39.503044329006045 40.0 40.0 40.0 40.0 40.0 26-27 39.484046656535455 40.0 40.0 40.0 40.0 40.0 28-29 39.50321318249089 40.0 40.0 40.0 40.0 40.0 30-31 39.47750396019063 40.0 40.0 40.0 40.0 40.0 32-33 39.47327365656578 40.0 40.0 40.0 40.0 40.0 34-35 39.47812640678414 40.0 40.0 40.0 40.0 40.0 36-37 39.46250504864173 40.0 40.0 40.0 40.0 40.0 38-39 39.403129944094324 40.0 40.0 40.0 40.0 40.0 40-41 39.44729402771735 40.0 40.0 40.0 40.0 40.0 42-43 39.435040515849394 40.0 40.0 40.0 40.0 40.0 44-45 39.34846296135461 40.0 40.0 40.0 40.0 40.0 46-47 39.32174017689526 40.0 40.0 40.0 40.0 40.0 48-49 39.39401235993227 40.0 40.0 40.0 40.0 40.0 50-51 39.35578900756256 40.0 40.0 40.0 40.0 40.0 52-53 39.311117509618924 40.0 40.0 40.0 40.0 40.0 54-55 39.21647400330468 40.0 40.0 40.0 40.0 40.0 56-57 39.236290750908594 40.0 40.0 40.0 40.0 40.0 58-59 39.19461514834799 40.0 40.0 40.0 40.0 40.0 60-61 39.10201738287098 40.0 40.0 40.0 40.0 40.0 62-63 39.37239187844288 40.0 40.0 40.0 40.0 40.0 64-65 39.37444503832069 40.0 40.0 40.0 40.0 40.0 66-67 39.38531303431533 40.0 40.0 40.0 40.0 40.0 68-69 39.3773316763125 40.0 40.0 40.0 40.0 40.0 70-71 39.38219740841585 40.0 40.0 40.0 40.0 40.0 72-73 39.38733829127149 40.0 40.0 40.0 40.0 40.0 74-75 39.391500333257056 40.0 40.0 40.0 40.0 40.0 76-77 39.379246278323954 40.0 40.0 40.0 40.0 40.0 78-79 39.36882692856546 40.0 40.0 40.0 40.0 40.0 80-81 39.37654236856939 40.0 40.0 40.0 40.0 40.0 82-83 39.349968840415755 40.0 40.0 40.0 40.0 40.0 84-85 39.34742586771601 40.0 40.0 40.0 40.0 40.0 86-87 39.31338534439762 40.0 40.0 40.0 40.0 40.0 88-89 39.348842028276636 40.0 40.0 40.0 40.0 40.0 90-91 39.43357682316872 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.01308456551749515 1101 2 0.05953638110560178 1101 3 0.13758577954275353 1101 4 -0.02280467028594302 1101 5 0.026316494815105784 1101 6 0.05167882622993858 1101 7 -0.07180975270765089 1101 8 -0.1647799838742614 1101 9 -0.023802022084886687 1101 10-11 -0.0580678303069746 1101 12-13 -0.047340155395211525 1101 14-15 -0.04374607459739366 1101 16-17 0.03496848533927022 1101 18-19 0.009532270306877422 1101 20-21 0.06848290167382487 1101 22-23 -0.010873329984626423 1101 24-25 0.17778501365830124 1101 26-27 0.04480767163362742 1101 28-29 0.015777121449119136 1101 30-31 0.09101874242050201 1101 32-33 0.12393064466970571 1101 34-35 -0.052486347607356265 1101 36-37 0.0524426153824038 1101 38-39 0.02069235595387653 1101 40-41 0.012996842987497814 1101 42-43 -0.034235320800270586 1101 44-45 0.06841375028055552 1101 46-47 -0.06759483735273619 1101 48-49 0.011087096737789182 1101 50-51 0.08033512531657294 1101 52-53 -0.022105537492514316 1101 54-55 -0.09593811891317472 1101 56-57 0.14290406878928508 1101 58-59 0.009891659462709868 1101 60-61 -0.018843343017920233 1101 62-63 0.009444887242445077 1101 64-65 -0.015806760272077724 1101 66-67 0.07870025007809289 1101 68-69 0.05198798820126882 1101 70-71 0.1995074101619423 1101 72-73 -0.004800460767377501 1101 74-75 0.055455317376505775 1101 76-77 -0.008456711951872364 1101 78-79 0.09630323400547525 1101 80-81 0.22654926359832928 1101 82-83 0.22278483929501647 1101 84-85 0.13850130458310161 1101 86-87 0.19415324277193946 1101 88-89 0.006348374417221692 1101 90-91 -0.022128800833804974 1102 1 0.13792038501275528 1102 2 0.038691320593358114 1102 3 0.15602090478902397 1102 4 -0.0998170541868717 1102 5 -0.02909442553389141 1102 6 -0.20317622583873174 1102 7 -0.13830285712645463 1102 8 -0.2517604698485556 1102 9 -0.1935177552681111 1102 10-11 -0.1673299562099615 1102 12-13 -0.1474785361063482 1102 14-15 0.025180419445192115 1102 16-17 -0.0695206787474163 1102 18-19 -0.12491983589445255 1102 20-21 -0.13374643503694017 1102 22-23 -0.03409900781556274 1102 24-25 -0.12401393558646845 1102 26-27 -0.09445844151019855 1102 28-29 -0.1308712508184442 1102 30-31 -0.06431946941926014 1102 32-33 -0.08104728473269063 1102 34-35 -0.06954273052327409 1102 36-37 -0.06785957998803127 1102 38-39 -0.10029919538774834 1102 40-41 -0.07400515490291326 1102 42-43 -0.015661805979917176 1102 44-45 -0.12681485767271283 1102 46-47 -0.06467617187367836 1102 48-49 -0.19106935720409268 1102 50-51 -0.16666032108118856 1102 52-53 -0.193142105678632 1102 54-55 -0.08707743419933678 1102 56-57 -0.02513607959386377 1102 58-59 -0.03351355820013424 1102 60-61 0.08652620838128655 1102 62-63 -0.11983206212173769 1102 64-65 -0.1517248875434163 1102 66-67 -0.19895312189349568 1102 68-69 -0.0523884105754675 1102 70-71 -0.09383442014029697 1102 72-73 -0.14407936911788255 1102 74-75 -0.3206709825274032 1102 76-77 -0.21558476851774344 1102 78-79 -0.10017911698597715 1102 80-81 -0.2873056278832138 1102 82-83 -0.1839430461640177 1102 84-85 -0.14447650968082115 1102 86-87 -0.05197628446649816 1102 88-89 -0.2924971264316767 1102 90-91 0.09340393344090359 1103 1 -0.04799729948347675 1103 2 0.0013036328213544834 1103 3 0.036267991690614565 1103 4 -0.07807410879868826 1103 5 0.061545052214242446 1103 6 0.050719604569799515 1103 7 -0.029535483828688314 1103 8 0.02077802048791 1103 9 -0.03663161178923957 1103 10-11 -0.01831151828586286 1103 12-13 -0.13746987870543848 1103 14-15 -0.06716421530607875 1103 16-17 0.05886623687801773 1103 18-19 -0.03704136708449823 1103 20-21 -0.09034536339109422 1103 22-23 -0.004912452525196898 1103 24-25 0.049654696544195076 1103 26-27 0.04476484923808499 1103 28-29 -0.07256833538019691 1103 30-31 -0.08574068707099514 1103 32-33 -0.0333454197969516 1103 34-35 0.010555615848765854 1103 36-37 0.0821869170094871 1103 38-39 -0.05685148438028165 1103 40-41 -0.05306462618302987 1103 42-43 0.004187275428407133 1103 44-45 0.04133656204915326 1103 46-47 0.007925402947599025 1103 48-49 -0.10083286025692928 1103 50-51 0.09178883763383539 1103 52-53 -0.028367195286342906 1103 54-55 0.10306992611049992 1103 56-57 -0.001139209841021227 1103 58-59 -0.09114228493773169 1103 60-61 -0.04213733122310259 1103 62-63 0.017723944424794524 1103 64-65 0.09834464638804974 1103 66-67 0.12743524896728076 1103 68-69 -0.09058692017693204 1103 70-71 0.11105099850298217 1103 72-73 0.03163576745218677 1103 74-75 0.1267531543405127 1103 76-77 0.17456778561679442 1103 78-79 -0.09599795045468085 1103 80-81 -0.00943011682793582 1103 82-83 -0.45835490479719 1103 84-85 -0.193454044366284 1103 86-87 -0.18633623957669698 1103 88-89 -0.00851879825830082 1103 90-91 -0.5299878794245956 1104 1 0.04769010685497932 1104 2 -0.025733510161835227 1104 3 -0.05636816532918232 1104 4 -0.18248831589369985 1104 5 -0.19085474683593873 1104 6 0.04869160537246131 1104 7 -0.02913557661854327 1104 8 -0.04090161825782701 1104 9 0.06631247378236793 1104 10-11 -0.00959467963063787 1104 12-13 0.043106016140306735 1104 14-15 -0.026191515733167137 1104 16-17 -0.1078068339966336 1104 18-19 -0.0641539374062603 1104 20-21 -0.09594477591174666 1104 22-23 -0.11195380805408917 1104 24-25 -0.019253158288066174 1104 26-27 0.023585421831207043 1104 28-29 -0.030486284667411212 1104 30-31 0.013612060974651286 1104 32-33 0.03968777997612705 1104 34-35 -0.010775784101959118 1104 36-37 -0.07053470047686261 1104 38-39 0.028088150390438216 1104 40-41 -0.03634566090050839 1104 42-43 -0.06827372440006485 1104 44-45 -0.1324801631711665 1104 46-47 -0.04762814475343191 1104 48-49 -0.003342640195903357 1104 50-51 0.03895409346313983 1104 52-53 -1.9685088119558714E-4 1104 54-55 -0.015635540682190197 1104 56-57 0.027717719573246313 1104 58-59 -0.20782522844304907 1104 60-61 0.06855957610476082 1104 62-63 -0.24359637701095238 1104 64-65 -0.13569408698003116 1104 66-67 -0.21377767450798046 1104 68-69 0.02401471565170965 1104 70-71 -0.08645147029648115 1104 72-73 -0.04116411092817884 1104 74-75 -0.20471445343045502 1104 76-77 -0.05073126434617592 1104 78-79 0.04054902300604368 1104 80-81 -0.12268435517825793 1104 82-83 0.17234770665162813 1104 84-85 0.01628757697388039 1104 86-87 -0.16585847100447637 1104 88-89 -0.32333780278319324 1104 90-91 -0.14106182962898117 1201 1 0.13390146637324563 1201 2 0.1304307843929422 1201 3 0.0457138645739974 1201 4 0.03429766375654708 1201 5 -0.154921469884151 1201 6 0.1014345464139268 1201 7 0.01544985661281828 1201 8 -0.051008376392545074 1201 9 -0.025059159175896184 1201 10-11 -0.09261597571442337 1201 12-13 0.04624368037250548 1201 14-15 -0.058934729250481155 1201 16-17 0.03260206841823532 1201 18-19 0.09564686178637771 1201 20-21 0.11053532863513738 1201 22-23 0.0415087836314143 1201 24-25 0.006120631421353551 1201 26-27 0.04649016112945503 1201 28-29 0.05380516807951352 1201 30-31 0.026181581616270932 1201 32-33 0.11429568105527466 1201 34-35 0.07829941750249247 1201 36-37 0.0653389583445474 1201 38-39 0.09775424137508537 1201 40-41 0.048747840454474556 1201 42-43 0.09086663275903817 1201 44-45 9.447609731978446E-4 1201 46-47 0.06492438465068773 1201 48-49 0.03199287002912854 1201 50-51 -0.014952451359320662 1201 52-53 0.12446703724936015 1201 54-55 0.0773434948639462 1201 56-57 0.04261554249179511 1201 58-59 0.10276060678454257 1201 60-61 0.04266196808332268 1201 62-63 -0.0021040168811410354 1201 64-65 -0.05349326318712855 1201 66-67 0.1022542547551808 1201 68-69 -0.047486244794519905 1201 70-71 -0.08077039483598725 1201 72-73 0.09483872166596541 1201 74-75 -0.16324358849286824 1201 76-77 0.060601093054586386 1201 78-79 -0.15272660555067574 1201 80-81 0.1537299047919518 1201 82-83 0.15776909880371193 1201 84-85 0.08856485838501271 1201 86-87 0.09452617065706903 1201 88-89 0.12579279285129275 1201 90-91 0.5144565650198487 1202 1 0.10250933457612632 1202 2 0.1147739569381443 1202 3 0.10258895621641528 1202 4 0.013190134587581781 1202 5 0.10083057619417701 1202 6 0.026968240878623817 1202 7 0.1898750650412424 1202 8 -0.015271103244096196 1202 9 0.14936604925252794 1202 10-11 0.13096674202279956 1202 12-13 -0.033198335189318584 1202 14-15 0.027131699369626006 1202 16-17 -0.06409453649263241 1202 18-19 -0.021860574587108772 1202 20-21 -0.05282808062113986 1202 22-23 0.0025980152945024315 1202 24-25 -0.10106227548646984 1202 26-27 0.026277543835760753 1202 28-29 -0.027408772995357822 1202 30-31 -0.06695189724349859 1202 32-33 -0.05854417247831378 1202 34-35 0.03757890620597948 1202 36-37 -0.28747920083122835 1202 38-39 -0.06113719367710502 1202 40-41 -0.07115743617722359 1202 42-43 -0.03806911790336187 1202 44-45 -0.08071369746622281 1202 46-47 0.04486571334560807 1202 48-49 0.06320491249238103 1202 50-51 0.07024021401429792 1202 52-53 0.05626027095752306 1202 54-55 -0.07952431676951477 1202 56-57 0.08709577251973144 1202 58-59 -0.03464195019891747 1202 60-61 0.10498300402979055 1202 62-63 -0.2394300825539588 1202 64-65 -0.023537465919964973 1202 66-67 -0.16865872762362244 1202 68-69 -0.14739094793052487 1202 70-71 0.004292467800404154 1202 72-73 -0.30890482090575233 1202 74-75 0.03659063959533171 1202 76-77 -0.22945859752996256 1202 78-79 -0.1850442565191699 1202 80-81 0.010141862599603257 1202 82-83 -0.17679826406865118 1202 84-85 -0.3426259871130526 1202 86-87 -0.08185717817965354 1202 88-89 -0.15471035180279813 1202 90-91 -0.6841097432238783 1203 1 0.10478602530476877 1203 2 0.030020640393082942 1203 3 0.020696132146902357 1203 4 0.029491675674499618 1203 5 -0.16022542454611965 1203 6 0.06609384466775481 1203 7 0.04354087052094968 1203 8 0.09299894520872698 1203 9 -0.05023971446290432 1203 10-11 -0.07417425154245905 1203 12-13 0.018936929906345767 1203 14-15 0.06501417286550293 1203 16-17 0.054785326225506026 1203 18-19 0.013417925382611884 1203 20-21 0.045786104672629335 1203 22-23 -0.008922883515239732 1203 24-25 0.07774844278700499 1203 26-27 0.011520559818187337 1203 28-29 -0.04758191307988113 1203 30-31 -0.13476067427410499 1203 32-33 -0.04415931392170336 1203 34-35 0.0038658250809575634 1203 36-37 -0.08166906222896841 1203 38-39 0.05284061719361688 1203 40-41 -0.06000459404000935 1203 42-43 -0.08972395879663964 1203 44-45 0.052962591788329405 1203 46-47 -0.08203913974267607 1203 48-49 -0.045491357137969146 1203 50-51 0.014701447624538844 1203 52-53 -0.04553279039522806 1203 54-55 0.022543909388751615 1203 56-57 -0.10601031603430044 1203 58-59 -0.017870721581218163 1203 60-61 -0.1962922090198873 1203 62-63 -0.02964704562446485 1203 64-65 -0.10299548816110615 1203 66-67 -0.04935529999493582 1203 68-69 0.09088524139784937 1203 70-71 0.10713221167098652 1203 72-73 0.07440999437380924 1203 74-75 0.11672173521996143 1203 76-77 -0.0741415825781715 1203 78-79 -0.08443806309738733 1203 80-81 -0.12620877865393965 1203 82-83 0.26067804641698444 1203 84-85 0.23536371582561344 1203 86-87 0.16023895598448945 1203 88-89 0.438329941237491 1203 90-91 0.5144565650198487 1204 1 0.005336712111976283 1204 2 0.07268506906966365 1204 3 -0.10319965732569614 1204 4 -0.05222101754841191 1204 5 -0.07733476962890506 1204 6 -0.06781941881752118 1204 7 -0.07410719662424015 1204 8 0.03458630687278941 1204 9 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0.022059816401238663 2401 34-35 -0.0034190281459132166 2401 36-37 -0.05580866294855724 2401 38-39 0.08565912373070006 2401 40-41 -0.11913415585632237 2401 42-43 0.021130746154952362 2401 44-45 -5.647785021167806E-4 2401 46-47 0.05879745433760064 2401 48-49 0.17859122348502154 2401 50-51 0.026101769482387738 2401 52-53 0.09948239388999269 2401 54-55 0.023840292099464477 2401 56-57 -0.0440029557237267 2401 58-59 0.006861756917757589 2401 60-61 0.05030744705205592 2401 62-63 -0.08119522113791788 2401 64-65 0.022408181287026707 2401 66-67 0.11262122390095186 2401 68-69 0.15503008874680546 2401 70-71 0.030010360984931594 2401 72-73 -0.1475520602706979 2401 74-75 0.026811220847605455 2401 76-77 0.14138530422280837 2401 78-79 0.28433347357836425 2401 80-81 -0.07540590193044494 2401 82-83 0.13647321133109358 2401 84-85 -0.07778162502371089 2401 86-87 -0.1607573175921999 2401 88-89 0.24206675511198483 2401 90-91 0.10743902116019655 2402 1 0.039353317582218494 2402 2 0.11680497346191743 2402 3 0.04345736085293339 2402 4 0.13891650508045927 2402 5 0.060973220180081 2402 6 0.17788566393775085 2402 7 0.01010753583731372 2402 8 0.05910386216682895 2402 9 0.09579879439702665 2402 10-11 0.08039389327496593 2402 12-13 -0.037803047255117406 2402 14-15 0.12187647097966448 2402 16-17 0.03326584052421566 2402 18-19 0.022384421885476513 2402 20-21 0.09877225831145608 2402 22-23 -0.06470600370034418 2402 24-25 0.023875329859080807 2402 26-27 0.048584338015885464 2402 28-29 0.05550076026307238 2402 30-31 0.0450500671470806 2402 32-33 0.1529967448476981 2402 34-35 0.10992756549870109 2402 36-37 0.1189742723925562 2402 38-39 -0.34482612387271416 2402 40-41 0.019071338848128505 2402 42-43 0.04646671851379125 2402 44-45 0.12492425061644497 2402 46-47 0.14205857915091968 2402 48-49 0.1683632074632726 2402 50-51 0.077248851060439 2402 52-53 0.13380039928030385 2402 54-55 0.17096307493122254 2402 56-57 0.25101526123197715 2402 58-59 0.07530858837241539 2402 60-61 0.08407982963585425 2402 62-63 0.18482802092975703 2402 64-65 0.00827067111637092 2402 66-67 0.0625996328855507 2402 68-69 0.06167345545004821 2402 70-71 -0.03905775692965108 2402 72-73 0.0354594930075649 2402 74-75 0.16982176491128342 2402 76-77 -0.02518500469698637 2402 78-79 0.1892641420465324 2402 80-81 0.01903726850323295 2402 82-83 1.91123670987281E-5 2402 84-85 -0.21211820040718976 2402 86-87 0.3104629286710363 2402 88-89 0.24711143899035903 2402 90-91 -0.5782752645540867 2403 1 -0.03418222996213416 2403 2 3.308154569481303E-5 2403 3 0.036389825374577356 2403 4 0.0699842756431508 2403 5 0.08152142518196115 2403 6 0.017130493020189874 2403 7 0.04311437704159715 2403 8 0.048016551260708695 2403 9 0.09558621813528845 2403 10-11 0.14403839240569738 2403 12-13 0.07754916868568529 2403 14-15 0.0697594406617057 2403 16-17 0.012119087459886657 2403 18-19 -0.0417406663232498 2403 20-21 0.012603011760930372 2403 22-23 0.03276896544483776 2403 24-25 0.02627785843287711 2403 26-27 0.05831449537650002 2403 28-29 0.03412642555383627 2403 30-31 0.07120611729673954 2403 32-33 0.08903971838225999 2403 34-35 0.10431149074737078 2403 36-37 0.07399742402665055 2403 38-39 0.10738270693841656 2403 40-41 0.08617105400079339 2403 42-43 0.022853469181498554 2403 44-45 0.14326900239074547 2403 46-47 -0.02408025613458875 2403 48-49 0.09827137995018376 2403 50-51 0.05238994536129837 2403 52-53 0.12236957936152493 2403 54-55 0.14332246559614958 2403 56-57 -0.001935963040416766 2403 58-59 0.0300890472746147 2403 60-61 0.05525800017806404 2403 62-63 0.14596767591843474 2403 64-65 0.12275243193573715 2403 66-67 -0.07727667064430932 2403 68-69 0.03354219686738702 2403 70-71 0.038961183442921765 2403 72-73 -0.03842112168521794 2403 74-75 0.19655707190922556 2403 76-77 0.06604201761704331 2403 78-79 -0.0024502681314899633 2403 80-81 0.11663738425584569 2403 82-83 -0.027414666910971164 2403 84-85 -0.28822477664333235 2403 86-87 0.08078532150998541 2403 88-89 0.26000536726075296 2403 90-91 0.3544565650198521 2404 1 -0.05531965654978421 2404 2 -0.07548081011364616 2404 3 -0.029048828906191204 2404 4 0.15781132836761458 2404 5 0.05980931778161391 2404 6 -0.00989699546224898 2404 7 0.09291338450953646 2404 8 0.08516591788333017 2404 9 0.1090512394380454 2404 10-11 0.10645093577223008 2404 12-13 0.11361413760086947 2404 14-15 0.05931107719025164 2404 16-17 0.060232364543907124 2404 18-19 0.04291689678856869 2404 20-21 0.08362439863229554 2404 22-23 0.14045748677861525 2404 24-25 0.02704326664572676 2404 26-27 0.08465403262518123 2404 28-29 0.08788406766343826 2404 30-31 0.01534131220723367 2404 32-33 0.06573494870434615 2404 34-35 0.04191086534188315 2404 36-37 0.05680333028562501 2404 38-39 0.11803567320892938 2404 40-41 0.04541209603620899 2404 42-43 0.06081043875074954 2404 44-45 0.11038864597995257 2404 46-47 0.09204221359733111 2404 48-49 0.10220547080829334 2404 50-51 0.11929108999849092 2404 52-53 0.06989311786266228 2404 54-55 0.12483000425491042 2404 56-57 0.15060856247952614 2404 58-59 0.10562479251102275 2404 60-61 0.10985223273026179 2404 62-63 0.018606772850851883 2404 64-65 0.07880890201531798 2404 66-67 0.06779841679522036 2404 68-69 0.08057226398860706 2404 70-71 0.048881797886970446 2404 72-73 0.04232741864085199 2404 74-75 0.14829156068385885 2404 76-77 0.09091965125752921 2404 78-79 0.21740239408219253 2404 80-81 0.14042452063716837 2404 82-83 0.12522086019951928 2404 84-85 0.2321444305460716 2404 86-87 0.1166029006409488 2404 88-89 0.25920058457679573 2404 90-91 0.5144565650198487 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 15.0 31 371.0 32 2557.0 33 4583.0 34 5996.0 35 7550.0 36 10759.0 37 15607.0 38 39139.0 39 271392.0 40 246105.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 31.010439118386156 13.738548588417975 23.310720209775624 31.94029208342024 2 30.55582232495604 17.636033076462 24.150817804594542 27.657326793987423 3 27.69263368395263 19.452749166492847 20.079990199876175 32.77462694967835 4 27.971784867060766 20.644193665335152 19.606405186128168 31.77761628147591 5 28.31722603522085 21.100394984720417 19.606041643904554 30.97633733615418 6 28.339739833199246 23.422627029138816 22.318788757668763 25.91884437999318 7 27.398629969175964 23.188384204584207 21.775808924072216 27.637176902167614 8 26.611474753093162 23.992755854415186 21.997636051212268 27.39813334127938 9 25.9600644954095 24.084300930018507 22.926330217820993 27.029304356750995 10-11 26.416879388949038 22.794392739962323 22.45792734002788 28.330800531060763 12-13 26.748709595182046 22.557004605396028 22.347179319090046 28.34710648033188 14-15 27.035098348877785 22.31738164529511 22.293460734943068 28.35405927088403 16-17 27.32297698626327 22.184947539539856 21.909815684833315 28.582259789363558 18-19 27.263712723937793 22.501547823611016 21.98331661352748 28.251422838923705 20-21 27.19046010919192 22.46545952979271 22.071964693067407 28.27211566794797 22-23 27.248565573092037 21.978515877193853 22.051106321410956 28.721812228303158 24-25 27.247986173879358 22.432268232037796 22.031323976863764 28.288421617219083 26-27 27.423213050056784 22.4852418743399 21.99209037303377 28.099454702569552 28-29 27.436539231948405 22.280134553051447 22.02503335684039 28.258292858159763 30-31 27.270688086797385 22.399095474309004 22.242326471861453 28.087889967032158 32-33 27.30511910329313 22.41332143123064 22.235877477501596 28.045681987974636 34-35 27.480234547203047 22.190156847059225 22.1510089104108 28.178599695326927 36-37 27.424047846665605 22.421438612668982 22.196636389132735 27.957877151532674 38-39 27.709969519656486 22.43940641199635 22.087792288695574 27.76283177965159 40-41 27.58052601553472 22.156996474701227 22.212947793397014 28.04952971636704 42-43 27.50504745551635 22.41532539577712 22.327395890333417 27.752231258373115 44-45 27.754290487891858 22.36945338720157 22.23628094207536 27.63997518283121 46-47 27.62717769680914 22.125125321366138 22.219084602968707 28.02861237885601 48-49 27.657023904285342 22.487707624293698 22.218912928961252 27.636355542459707 50-51 27.81293452330711 22.52735414454716 22.155413299689474 27.504298032456255 52-53 27.863624469655907 22.270947423508964 22.159154626843357 27.70627347999178 54-55 27.83025328498046 22.458420272669727 22.212748525167473 27.498577917182338 56-57 27.86075123936425 22.494101071365908 22.142129480501456 27.50301820876839 58-59 27.951751366192717 22.284897093881494 22.10515878280713 27.658192757118655 60-61 28.081504683419972 22.41693218024579 22.0806482524016 27.420914883932635 62-63 28.05020361322687 22.538684899048835 22.058664497374735 27.352446990349556 64-65 28.13059324069188 22.28022782293761 21.996939220921284 27.592239715449217 66-67 28.112846247179895 22.359359096493968 22.065129259863408 27.462665396462725 68-69 28.107560043091613 22.60915485519951 21.920322764622632 27.362962337086245 70-71 28.312555351181963 22.38666074687978 21.828039587400248 27.472744314538012 72-73 28.359612038120186 22.470735178784093 21.763573516558093 27.406079266537624 74-75 28.194190412013864 22.4613135348479 21.880715729688102 27.46378032345013 76-77 28.346736168865288 22.32363759103895 21.743563333182543 27.586062906913217 78-79 28.396498590256375 22.364517080847158 21.537931938889255 27.701052390007213 80-81 28.704792199709562 22.32803501042212 21.343613857962794 27.623558931905517 82-83 28.95011479858067 21.8860117622503 21.361130551155966 27.802742888013064 84-85 29.175786429792467 21.842698065278864 20.80743853146518 28.174076973463485 86-87 29.4408413277485 21.217853443228393 20.65036256361753 28.69094266540558 88-89 28.47782442051469 21.03668552655594 20.78846504836649 29.697025004562878 90-91 24.758983347940404 23.58019281332165 22.585451358457494 29.075372480280453 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 3.0 11 4.0 12 5.0 13 7.5 14 13.5 15 20.0 16 28.0 17 40.0 18 69.0 19 101.0 20 128.0 21 172.0 22 231.5 23 322.0 24 457.5 25 613.0 26 776.5 27 1001.0 28 1294.5 29 1595.5 30 1927.0 31 2334.0 32 2810.0 33 3304.5 34 4041.5 35 5003.5 36 5985.0 37 7048.5 38 8268.5 39 9757.5 40 11395.5 41 13237.5 42 15097.5 43 16933.5 44 19135.5 45 21454.0 46 23694.5 47 26088.5 48 28351.0 49 30329.0 50 31994.0 51 33763.5 52 35483.5 53 36772.5 54 38023.0 55 38974.5 56 39551.5 57 39990.0 58 40104.0 59 40000.0 60 39821.0 61 39087.0 62 37995.5 63 36527.5 64 34649.0 65 32244.5 66 29244.5 67 26593.0 68 23924.0 69 20816.5 70 17684.5 71 14746.0 72 12006.5 73 9502.0 74 7356.5 75 5642.0 76 4333.5 77 3174.5 78 2287.0 79 1642.5 80 1135.0 81 794.0 82 551.0 83 358.5 84 235.0 85 164.0 86 113.5 87 78.5 88 52.0 89 30.0 90 17.5 91 12.0 92 11.0 93 6.5 94 3.5 95 3.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.02317596850716965 3 0.0 4 1.6554263219406893E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06545649233243005 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 1.6140538900312804E-4 64-65 1.7530204542426603E-4 66-67 3.8335988100509293E-4 68-69 6.336961595900408E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.878689690599439E-4 82-83 6.138923846649682E-4 84-85 0.0 86-87 0.0011259612894508688 88-89 0.0 90-91 0.004381928925112835 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 4124.0 32-33 9711.0 34-35 11169.0 36-37 13290.0 38-39 14846.0 40-41 16594.0 42-43 17814.0 44-45 19308.0 46-47 20246.0 48-49 21300.0 50-51 22051.0 52-53 22616.0 54-55 23219.0 56-57 23813.0 58-59 24110.0 60-61 23878.0 62-63 24699.0 64-65 24303.0 66-67 24221.0 68-69 24022.0 70-71 23443.0 72-73 23390.0 74-75 22681.0 76-77 21925.0 78-79 20934.0 80-81 20077.0 82-83 19088.0 84-85 18360.0 86-87 17358.0 88-89 16108.0 90-91 15376.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.76409813512988 #Duplication Level Percentage of deduplicated Percentage of total 1 99.77729951868481 99.54192300840319 2 0.21412072463956539 0.4272312197141344 3 0.006335517354303935 0.018961715252147886 4 0.0012882315040160036 0.005140770167496742 5 5.57323671244305E-4 0.0027800446715523855 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 2.064561732055147E-4 0.001853722253185051 >10 1.9222797285647862E-4 0.00210951953832079 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002814224747299172 0.0 0.0 0.0 0.0 2 0.002814224747299172 0.0 0.0 0.0 0.0 3 0.002979767379493241 0.0 0.0 0.0 0.0 4 0.0031453100116873097 0.0 0.0 0.0 0.0 5 0.0031453100116873097 0.0 0.0 0.0 0.0 6 0.0038074805404635856 0.0 0.0 0.0 0.0 7 0.004469651069239861 0.0 0.0 0.0 0.0 8 0.011587984253584826 0.0 0.0 0.0 0.0 9 0.01241569741455517 0.0 0.0 0.0 0.0 10-11 0.01241569741455517 0.0 0.0 0.0 0.0 12-13 0.01241569741455517 0.0 8.277131609703446E-5 0.0 0.0 14-15 0.01241569741455517 0.0 1.6554263219406893E-4 0.0 0.0 16-17 0.01241569741455517 0.0 1.6554263219406893E-4 0.0 0.0 18-19 0.01241569741455517 0.0 1.6554263219406893E-4 0.0 1.6554263219406893E-4 20-21 0.01241569741455517 0.0 3.3108526438813785E-4 0.0 1.6554263219406893E-4 22-23 0.01241569741455517 0.0 4.966278965822068E-4 0.0 2.483139482911034E-4 24-25 0.01241569741455517 0.0 4.966278965822068E-4 0.0 3.3108526438813785E-4 26-27 0.012664011362846273 0.0 4.966278965822068E-4 0.0 5.793992126792413E-4 28-29 0.012746782678943308 0.0 4.966278965822068E-4 0.0 7.449418448733103E-4 30-31 0.012746782678943308 0.0 4.966278965822068E-4 0.0 9.104844770673791E-4 32-33 0.012912325311137377 0.0 4.966278965822068E-4 0.0 0.0012415697414555169 34-35 0.013243410575525515 0.0 4.966278965822068E-4 0.0 0.0013243410575525514 36-37 0.013408953207719584 0.0 4.966278965822068E-4 0.0 0.0013243410575525514 38-39 0.013408953207719584 0.0 4.966278965822068E-4 0.0 0.0013243410575525514 40-41 0.013905581104301791 0.0 4.966278965822068E-4 0.0 0.0013243410575525514 42-43 0.014402209000883997 0.0 4.966278965822068E-4 0.0 0.0013243410575525514 44-45 0.014402209000883997 0.0 4.966278965822068E-4 0.0 0.0013243410575525514 46-47 0.014484980316981031 0.0 4.966278965822068E-4 0.0 0.0013243410575525514 48-49 0.0146505229491751 0.0 4.966278965822068E-4 0.0 0.0013243410575525514 50-51 0.014733294265272135 0.0 4.966278965822068E-4 0.0 0.0013243410575525514 52-53 0.014733294265272135 0.0 4.966278965822068E-4 0.0 0.0013243410575525514 54-55 0.014733294265272135 0.0 4.966278965822068E-4 0.0 0.0013243410575525514 56-57 0.014898836897466204 0.0 4.966278965822068E-4 0.0 0.0014898836897466205 58-59 0.014898836897466204 0.0 4.966278965822068E-4 0.0 0.001572655005843655 60-61 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0.0 0.0018209689541347582 >>END_MODULE