##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-35B_S45_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1437248
Sequences flagged as poor quality	0
Sequence length	61
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.506342677116265	40.0	40.0	40.0	40.0	40.0
2	39.23624593667898	40.0	40.0	40.0	40.0	40.0
3	39.19477223137552	40.0	40.0	40.0	40.0	40.0
4	39.08995107316204	40.0	40.0	40.0	40.0	40.0
5	39.1231408914815	40.0	40.0	40.0	40.0	40.0
6	39.23917097118938	40.0	40.0	40.0	40.0	40.0
7	39.26695740748987	40.0	40.0	40.0	40.0	40.0
8	39.28512824509062	40.0	40.0	40.0	40.0	40.0
9	39.29058868058957	40.0	40.0	40.0	40.0	40.0
10	39.300451974885334	40.0	40.0	40.0	40.0	40.0
11	39.29715122233602	40.0	40.0	40.0	40.0	40.0
12	39.302483635392086	40.0	40.0	40.0	40.0	40.0
13	39.13305984770896	40.0	40.0	40.0	40.0	40.0
14	39.29989535556842	40.0	40.0	40.0	40.0	40.0
15	39.21195228659215	40.0	40.0	40.0	40.0	40.0
16	39.38693948434786	40.0	40.0	40.0	40.0	40.0
17	39.3328639177094	40.0	40.0	40.0	40.0	40.0
18	39.420545375606714	40.0	40.0	40.0	40.0	40.0
19	39.369016342343144	40.0	40.0	40.0	40.0	40.0
20	39.366430845616065	40.0	40.0	40.0	40.0	40.0
21	39.353915260275194	40.0	40.0	40.0	40.0	40.0
22	39.410067016965755	40.0	40.0	40.0	40.0	40.0
23	39.40102195306586	40.0	40.0	40.0	40.0	40.0
24	39.40574208487331	40.0	40.0	40.0	40.0	40.0
25	39.36855991450327	40.0	40.0	40.0	40.0	40.0
26	39.335215634323376	40.0	40.0	40.0	40.0	40.0
27	39.350675735850736	40.0	40.0	40.0	40.0	40.0
28	39.35608607561117	40.0	40.0	40.0	40.0	40.0
29	39.35744700984103	40.0	40.0	40.0	40.0	40.0
30	39.354644431580354	40.0	40.0	40.0	40.0	40.0
31	39.3059819877989	40.0	40.0	40.0	40.0	40.0
32	39.32195696219441	40.0	40.0	40.0	40.0	40.0
33	39.32689556708376	40.0	40.0	40.0	40.0	40.0
34	39.317799015897045	40.0	40.0	40.0	40.0	40.0
35	39.31677205325734	40.0	40.0	40.0	40.0	40.0
36	39.28859876653159	40.0	40.0	40.0	40.0	40.0
37	39.29600736963975	40.0	40.0	40.0	40.0	40.0
38	39.274324264149264	40.0	40.0	40.0	40.0	40.0
39	38.04473131985572	40.0	40.0	40.0	40.0	40.0
40	39.242917019192234	40.0	40.0	40.0	40.0	40.0
41	39.27042514583426	40.0	40.0	40.0	40.0	40.0
42	39.23688048270027	40.0	40.0	40.0	40.0	40.0
43	39.25666829941666	40.0	40.0	40.0	40.0	40.0
44	39.20769971501091	40.0	40.0	40.0	40.0	40.0
45	39.10574932092443	40.0	40.0	40.0	40.0	40.0
46	39.07212951418266	40.0	40.0	40.0	40.0	40.0
47	39.15690541924567	40.0	40.0	40.0	40.0	40.0
48	39.21607126953734	40.0	40.0	40.0	40.0	40.0
49	39.18966803223939	40.0	40.0	40.0	40.0	40.0
50	39.17317818497573	40.0	40.0	40.0	40.0	40.0
51	39.136174132787104	40.0	40.0	40.0	40.0	40.0
52	39.13522231375518	40.0	40.0	40.0	40.0	40.0
53	39.061216992474506	40.0	40.0	40.0	40.0	40.0
54	38.97702553769426	40.0	40.0	40.0	40.0	40.0
55	39.01663735138264	40.0	40.0	40.0	40.0	40.0
56	39.035192256312065	40.0	40.0	40.0	40.0	40.0
57	38.972511355034065	40.0	40.0	40.0	40.0	40.0
58	38.95773867836309	40.0	40.0	40.0	40.0	40.0
59	38.9248619584094	40.0	40.0	40.0	40.0	40.0
60	38.8950021151534	40.0	40.0	40.0	40.0	40.0
61	38.80673899006991	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.02300265816267455
1101	2	-0.02359487257960069
1101	3	0.0868153321269034
1101	4	-0.033537154523600066
1101	5	0.04278764857149042
1101	6	0.03960466718954336
1101	7	-0.0357388211164249
1101	8	-0.017721189953675776
1101	9	0.03187694399400698
1101	10	0.03602886799664162
1101	11	0.04797376969046496
1101	12	-0.08437209589529004
1101	13	-0.05982231197804566
1101	14	0.08786874486835217
1101	15	0.03871024298497616
1101	16	-0.013735512846913878
1101	17	0.05949687059909081
1101	18	-0.04074031990785443
1101	19	-0.03660278443987153
1101	20	-0.013810686544495354
1101	21	0.022243069670935256
1101	22	-0.030378547226362684
1101	23	0.03814205260328407
1101	24	-0.016970293945746562
1101	25	0.00874300285801155
1101	26	-0.04513546301212301
1101	27	-0.0031963333735944843
1101	28	0.02832809395869873
1101	29	0.0075365497668258286
1101	30	-0.039550384425652396
1101	31	0.02119230267422978
1101	32	0.05193182667044027
1101	33	0.059737836172132575
1101	34	0.09805532253372462
1101	35	0.0061373266346365085
1101	36	-0.0581661869336898
1101	37	-0.0813379912281249
1101	38	-0.06549583769832168
1101	39	1.2156314598395355
1101	40	-0.03363531410944631
1101	41	-0.055812040897485815
1101	42	0.011685917918427435
1101	43	0.009607067539754155
1101	44	0.05759336161440842
1101	45	-0.02703439020768883
1101	46	0.06280656154542896
1101	47	0.023676087167686433
1101	48	0.04361213867192504
1101	49	-0.0019128454501142755
1101	50	0.007845174146751788
1101	51	0.1679355473305435
1101	52	0.002829433517298696
1101	53	0.08982867886694379
1101	54	0.14961909076948388
1101	55	0.004380307588768062
1101	56	0.03638545343112298
1101	57	0.02672577346000793
1101	58	0.031278749409800355
1101	59	0.046383441995430985
1101	60	-0.026072420653456163
1101	61	-0.09033799053057834
1102	1	0.014761504210177634
1102	2	-0.05668219870955937
1102	3	-0.010983576078857027
1102	4	-0.018662904053400098
1102	5	-0.11448710779095705
1102	6	-0.04518863190970279
1102	7	-0.011448542600504652
1102	8	-0.03751003085876903
1102	9	-0.00846770071609626
1102	10	-0.038909603682355964
1102	11	-0.007852593053968349
1102	12	-0.023843803163693167
1102	13	-0.0024069863108451273
1102	14	-0.05017839948185099
1102	15	-0.04016918621640997
1102	16	-0.05385214661922788
1102	17	0.0012572984958723055
1102	18	-0.09299919132110546
1102	19	0.009771046044662057
1102	20	-0.10691927123118461
1102	21	-0.10976047442026271
1102	22	-0.0566379940957944
1102	23	-0.1126905523617765
1102	24	-0.020577659110877278
1102	25	-0.03739364383492472
1102	26	-0.06658028483317935
1102	27	-0.09889503245700126
1102	28	-0.13834790156270316
1102	29	-0.054333413236619776
1102	30	-0.07208930493801091
1102	31	0.006945363084582823
1102	32	-0.10178966564429714
1102	33	-0.06701864833351578
1102	34	-0.03550985311987631
1102	35	-0.0567650504261934
1102	36	-0.2073325149223706
1102	37	-0.11955640586320726
1102	38	-0.01351870393808241
1102	39	1.1914117561376898
1102	40	-0.10484268069730973
1102	41	-0.007331636453258739
1102	42	-0.053202662795854394
1102	43	-0.1325107646939898
1102	44	-0.07667157174480366
1102	45	-0.09355686210833625
1102	46	-0.05637370730904223
1102	47	-0.1448342919164034
1102	48	-0.17306227500019844
1102	49	-0.1495489916609145
1102	50	-0.06613707432190097
1102	51	-0.056430585070430084
1102	52	-0.05750791409398204
1102	53	-0.11442194671232642
1102	54	-0.07899070332990021
1102	55	-0.06859461176967585
1102	56	-0.10840261526943351
1102	57	-0.13566387851255968
1102	58	-0.037418624851433435
1102	59	-0.0917964279335024
1102	60	-0.10219568808388857
1102	61	-0.09527710430934633
1103	1	0.035840763172899415
1103	2	0.060786098420884116
1103	3	0.010534794883412246
1103	4	-0.03547870937388353
1103	5	0.07962901737328565
1103	6	0.01889874761224064
1103	7	-0.02977638452319553
1103	8	0.07007127923903766
1103	9	0.032291759680788346
1103	10	0.0513335611778345
1103	11	0.07634543817269446
1103	12	0.05248071642104435
1103	13	0.11284673267780931
1103	14	0.11906839539597769
1103	15	-0.014330118881261455
1103	16	0.01302581602160302
1103	17	0.08811146774380063
1103	18	0.04767971499627066
1103	19	-0.022308232124501615
1103	20	0.03281324704600053
1103	21	0.05340729310194803
1103	22	-0.0019056584681038657
1103	23	0.07484077579385229
1103	24	0.09847742910883284
1103	25	0.0017012582103390628
1103	26	0.07923239059036291
1103	27	0.01825824135644183
1103	28	0.07514940708746565
1103	29	0.04897013253629012
1103	30	0.009496650106115112
1103	31	0.11723591136826172
1103	32	0.05873439155474358
1103	33	0.051009836703727274
1103	34	0.1303106630513824
1103	35	0.07254288872629644
1103	36	0.07806618257954767
1103	37	0.10604585733359784
1103	38	0.14533062679271325
1103	39	1.3389081963553764
1103	40	0.07815442063625966
1103	41	0.1350050494713173
1103	42	0.10034681959051994
1103	43	0.17550784973818168
1103	44	0.06810546217037938
1103	45	0.02503388211871993
1103	46	0.05700870162323213
1103	47	0.0718576883393709
1103	48	0.15310529782140492
1103	49	0.06371332051608647
1103	50	0.12549125164080266
1103	51	0.06828324835096566
1103	52	0.1609932377276735
1103	53	0.1464617981974996
1103	54	0.21136664571405106
1103	55	0.1401956120757646
1103	56	0.15331792797913835
1103	57	0.15027674194591611
1103	58	0.09320137794291838
1103	59	0.1194691674153745
1103	60	0.14030297217259857
1103	61	0.24087555904051072
1104	1	0.0423919263370891
1104	2	0.04464704661829444
1104	3	0.015742415675831012
1104	4	0.009277162954646201
1104	5	-0.13182202325864978
1104	6	0.07127868384788627
1104	7	-0.07219760104244699
1104	8	0.11163484433281212
1104	9	0.05373676292254004
1104	10	0.008907140011878312
1104	11	0.04904780970714029
1104	12	0.03961780384126001
1104	13	-0.10527220390877545
1104	14	-0.0055524089152498846
1104	15	0.009028707307329853
1104	16	-0.04537589645586593
1104	17	-0.05193256356735532
1104	18	-0.021010840503109307
1104	19	-0.06009805659407874
1104	20	0.08313214318504691
1104	21	0.008495653132641223
1104	22	-0.0071883748773160505
1104	23	0.012803245829957177
1104	24	-0.029926409838580526
1104	25	-0.023847237200648408
1104	26	-0.007533159406953871
1104	27	0.005539943603068309
1104	28	-0.02911974155129826
1104	29	0.06763548267517905
1104	30	-0.06906042851082361
1104	31	-0.006749554284041892
1104	32	-0.10301784941742653
1104	33	-0.057684748679243114
1104	34	9.428491814489348E-4
1104	35	0.03571703917924651
1104	36	-0.1460020369554229
1104	37	0.02957881613063762
1104	38	-0.07700333010686222
1104	39	1.2013248476559397
1104	40	-0.1255718089986999
1104	41	-0.1023718234579789
1104	42	-0.024197773822479007
1104	43	0.03543928223540149
1104	44	-0.14696912785215943
1104	45	-0.0820606461618425
1104	46	-0.015790178931609944
1104	47	0.027932700681816414
1104	48	-0.06160645397345377
1104	49	-0.11505885871410726
1104	50	-0.036685168038111726
1104	51	0.02226822327943978
1104	52	-0.04298801959600951
1104	53	-0.052843455819001406
1104	54	-0.01860104086510006
1104	55	-0.07905645045137533
1104	56	-0.02938896416273451
1104	57	0.010221186059908405
1104	58	-0.12653620377003705
1104	59	0.03686510422204492
1104	60	-0.10936355867757186
1104	61	0.06414248603864792
1201	1	0.025374777813532035
1201	2	0.10102196423056142
1201	3	0.054388941140317115
1201	4	0.012073521268533227
1201	5	3.735429454607697E-4
1201	6	0.028847889541097516
1201	7	0.02354770359917069
1201	8	0.05644174530721813
1201	9	0.0689966776549511
1201	10	0.10184606621028536
1201	11	-0.032691209715579816
1201	12	-0.0018492024706802113
1201	13	0.009234493991009174
1201	14	-0.014312970873795905
1201	15	-0.04934522610557224
1201	16	0.041795888641303236
1201	17	0.032752002761448296
1201	18	0.03559732345292588
1201	19	0.026514085724421932
1201	20	3.0015058023025176E-5
1201	21	0.01973304261431963
1201	22	0.02946971158971934
1201	23	0.04452883085706105
1201	24	-0.010779056043404012
1201	25	0.059985989251302385
1201	26	0.04730403946960138
1201	27	0.02972742244946147
1201	28	0.14460480732021352
1201	29	0.0049198643367063255
1201	30	0.0937982178958876
1201	31	0.11820108730113077
1201	32	0.028583560229215266
1201	33	0.01478750669599549
1201	34	0.03414992888526314
1201	35	0.04931843695017335
1201	36	0.06961110589153918
1201	37	0.060249689767161385
1201	38	0.01887112286829762
1201	39	1.3070476032954232
1201	40	0.11832704558918294
1201	41	0.06840866297105919
1201	42	0.08844963341338996
1201	43	0.027384211604626785
1201	44	0.0598084876242595
1201	45	0.02822980398689623
1201	46	0.06887991387705483
1201	47	0.04891782041865156
1201	48	0.0024667063308001502
1201	49	0.09967177052033094
1201	50	0.06602301313770198
1201	51	0.0688091261895778
1201	52	0.15095789248811542
1201	53	0.07589516576747712
1201	54	0.14428729481368663
1201	55	0.055831932730242784
1201	56	0.09319507132188676
1201	57	-0.006856083239604516
1201	58	0.04279841510877702
1201	59	0.0794873943337322
1201	60	0.14018627773453574
1201	61	0.10293708674762314
1202	1	0.03594142367734321
1202	2	0.03923325179742676
1202	3	-0.08306345151257943
1202	4	-0.02399059367537859
1202	5	0.05375304713750495
1202	6	0.08591376120187988
1202	7	0.06676250582292198
1202	8	-0.030506283968072978
1202	9	-0.07609340171674006
1202	10	0.02629190441149376
1202	11	0.04001227414630648
1202	12	0.083881892735171
1202	13	-0.0354620059075188
1202	14	0.024554025307836014
1202	15	-0.02110132858206981
1202	16	-0.037203587194497345
1202	17	-0.06370719329995467
1202	18	0.039112638954890144
1202	19	-0.03518900663733859
1202	20	0.009499218600360848
1202	21	0.021407808845651743
1202	22	-0.01045878530099742
1202	23	0.010703069207721683
1202	24	0.003076153265212156
1202	25	0.058270765184047946
1202	26	-0.022225103767979704
1202	27	-0.07103742823431247
1202	28	0.04140909931325609
1202	29	0.05430853232267907
1202	30	0.06346255616705321
1202	31	0.058316151268257954
1202	32	-0.0427176362219015
1202	33	0.054614953084893614
1202	34	0.013214786465688633
1202	35	0.017974904666843372
1202	36	-0.18400815331795428
1202	37	-0.21073576620535306
1202	38	-0.17840184963194616
1202	39	1.1167732718823302
1202	40	-0.1604673372448815
1202	41	-0.06629937117422458
1202	42	-0.08966006170482643
1202	43	-0.07876397754498043
1202	44	-0.10538819532244759
1202	45	-0.08418182269190311
1202	46	0.010307317136017957
1202	47	-0.07384028622612959
1202	48	-0.033432754629693306
1202	49	-0.05507568837474963
1202	50	-0.11486001958920866
1202	51	-0.03225868782367769
1202	52	0.03959365331983378
1202	53	-0.020067245703955905
1202	54	-0.055876248931440387
1202	55	-0.04629380664846394
1202	56	-0.010601294416574092
1202	57	-0.12823994069479028
1202	58	0.007447110781079402
1202	59	-0.020982208235771793
1202	60	-0.1260454766047303
1202	61	-0.06888263802806449
1203	1	0.037442714991904325
1203	2	0.07317505489231735
1203	3	-0.017300015503160182
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2403	6	0.04200774371917504
2403	7	0.027867093795549636
2403	8	0.10779484285054508
2403	9	0.06163679009745948
2403	10	0.035187294908922695
2403	11	0.03571665860223305
2403	12	0.07387673142009987
2403	13	0.07270267781856177
2403	14	0.1539442992137552
2403	15	0.11171670834492176
2403	16	0.01193616426772337
2403	17	-0.014039762450714477
2403	18	0.09223523457378491
2403	19	0.090427067854705
2403	20	-0.0043116541394567776
2403	21	0.0393577157090661
2403	22	0.06633676809982347
2403	23	0.033364315286583235
2403	24	0.013113413771485227
2403	25	0.029502843706495696
2403	26	0.04057030910101389
2403	27	0.06773422575285082
2403	28	0.002352212696017375
2403	29	0.048748913261782434
2403	30	0.04611568349925932
2403	31	0.08237723524620577
2403	32	0.07771852915391975
2403	33	-0.013798796877672714
2403	34	0.002339423906299487
2403	35	0.06016674704885361
2403	36	-0.035099026715421644
2403	37	-0.02646096424659561
2403	38	0.04795420908060777
2403	39	1.2563679569608794
2403	40	0.022738405065553025
2403	41	0.0032668180430661664
2403	42	0.11916768561894742
2403	43	0.12391912338564737
2403	44	0.07317706086530507
2403	45	0.1315820901055531
2403	46	0.05792333565020158
2403	47	0.12262340068305377
2403	48	0.16454292162099904
2403	49	0.07957532980572779
2403	50	0.09589524502067093
2403	51	0.10061845858953689
2403	52	-0.040327656687303204
2403	53	0.09864437105205326
2403	54	0.04859898507459803
2403	55	-0.01565473719619348
2403	56	0.11271733918348303
2403	57	0.052774640362812875
2403	58	0.08821591772655779
2403	59	0.056374689557465274
2403	60	-0.06766781278778211
2403	61	0.00722415284942457
2404	1	0.029368531621067007
2404	2	-0.02457127432990802
2404	3	-0.03192043978268089
2404	4	0.015978127909114903
2404	5	0.021922023344622232
2404	6	0.022298518161164793
2404	7	0.013679662023996286
2404	8	0.019769860389530436
2404	9	0.05949759538968635
2404	10	-0.011388900882337794
2404	11	0.054351180842282076
2404	12	0.0599423512279742
2404	13	0.012811965873567033
2404	14	-0.05699247159075327
2404	15	-0.01291721956091152
2404	16	-0.03800241098448254
2404	17	-0.0543897456520952
2404	18	0.0011309472999911918
2404	19	-0.04444411377771473
2404	20	-0.022707421499482905
2404	21	2.9974565656942787E-4
2404	22	0.01127956598431723
2404	23	-0.07257912901296493
2404	24	-0.03128192264517082
2404	25	-0.09288588967741873
2404	26	-0.005885794415256385
2404	27	0.046018969030079404
2404	28	-0.13383242713438648
2404	29	-0.09541389755101193
2404	30	-0.04816982363019662
2404	31	-0.011942583961818798
2404	32	0.011484780161069352
2404	33	-0.13568898392956186
2404	34	-0.018954488661215407
2404	35	-0.040619469299009836
2404	36	0.015756988161321317
2404	37	0.04350258938185192
2404	38	-0.024956098533309046
2404	39	1.2013632441560418
2404	40	0.14703636053541658
2404	41	-0.06177683284379043
2404	42	0.05189497505665486
2404	43	-0.013124339601731094
2404	44	0.026313501416055374
2404	45	0.09183787848172642
2404	46	-0.01767393449716792
2404	47	0.006860778995161354
2404	48	0.04115382775601972
2404	49	-0.0017806508610505034
2404	50	0.0018166315554282164
2404	51	-0.028076957378033285
2404	52	-0.03470639872072212
2404	53	-0.020453874845046016
2404	54	-0.05569768369232264
2404	55	0.09465532757806727
2404	56	-0.03635618363557569
2404	57	-0.04335085197977406
2404	58	-0.0503201206784567
2404	59	-0.022691164103562755
2404	60	-0.032323308285853614
2404	61	-0.058173443466934316
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	5.0
17	47.0
18	121.0
19	158.0
20	213.0
21	284.0
22	445.0
23	588.0
24	988.0
25	1267.0
26	1654.0
27	2170.0
28	2944.0
29	3550.0
30	4434.0
31	5643.0
32	7766.0
33	9263.0
34	12071.0
35	15748.0
36	23597.0
37	34928.0
38	95626.0
39	685690.0
40	528048.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	30.950677962328005	14.24910662599635	22.849362114262814	31.950853297412834
2	30.92099768369006	17.173475467159623	23.666033764671816	28.239493084478497
3	27.710040299238546	19.380440953822863	19.765830253373114	33.143688493565485
4	27.91242968317858	20.21847353791455	19.734979074548875	32.13411770435799
5	28.275774257469834	20.596793316115242	19.484459188671686	31.642973237743245
6	28.958189540009798	22.90258883644298	22.034888898784345	26.10433272476288
7	27.887810593578838	22.72398361312731	21.418850469786705	27.969355323507145
8	26.431207418622254	23.978255666384644	21.62104243665672	27.969494478336376
9	26.014995324397738	23.89114474328717	22.81074664915171	27.28311328316338
10	26.945245357794896	22.216694683172285	22.339220510308593	28.49883944872423
11	26.7541161998486	22.655589014561162	21.989106959967938	28.601187825622297
12	27.30642171705927	22.676810126018612	21.967259651778956	28.049508505143162
13	26.80720376719954	21.51521518902792	22.16360711582135	29.5139739279512
14	27.131851983791243	22.102866032862806	22.01366778732689	28.75161419601906
15	27.16984125217082	22.10537081978893	22.023756512446006	28.701031415594247
16	27.408561361713495	22.187611323863383	21.567954869305783	28.83587244511733
17	27.498246649151714	21.802848221044663	21.517719975954044	29.181185153849583
18	27.927956761811462	22.313128979828114	21.73292291935699	28.025991339003433
19	27.233226276884714	21.885506189606804	22.196169345860977	28.685098187647505
20	27.317832413056063	22.00817117157234	21.700360689317364	28.973635726054237
21	27.026024736162448	22.247447900431936	22.451448880081934	28.275078483323686
22	27.915432827180837	21.50491773166496	21.73396658057621	28.84568286057799
23	27.429225853854035	22.179609921182706	21.669885781716168	28.721278443247094
24	27.37648617357617	22.698031237476066	21.75650966291134	28.168972926036425
25	27.568171950839382	21.620346662510574	21.652491428062522	29.158989958587522
26	27.653613015986107	21.918485884134125	22.017216235472237	28.410684864407536
27	27.48154806964421	22.478584071781626	21.74315079930534	28.296717059268822
28	27.517380438170726	22.011650042303067	21.561414592332014	28.909554927194193
29	27.645750768134658	22.84268268245981	21.588480206617092	27.923086342788437
30	27.953909137462706	22.77707118047825	21.75177839871755	27.517241283341498
31	27.469093712428194	22.102239836131275	22.272843656766263	28.15582279467427
32	27.752134635080377	22.198395823128646	21.623338491339002	28.426131050451975
33	27.398561277219574	23.27567912822222	21.783242546340333	27.542517048217878
34	27.783235739413097	22.512468272698936	21.462266776506212	28.24202921138175
35	27.856987798904576	23.157450906176248	21.36931135058111	27.616249944338065
36	28.3642767288596	23.15898160929777	21.386218662332457	27.090522999510174
37	27.788036581021508	22.777627799795162	21.274129447388344	28.16020617179499
38	27.991202631696133	23.145901055350222	21.612206105000666	27.250690207952978
39	28.19883278625425	24.027627074705556	21.1211542590663	26.652385879973888
40	27.970468562140983	23.172897092220687	20.93006913211916	27.926565213519172
41	28.15624025916195	23.543396825043416	20.837461593267133	27.462901322527493
42	28.11442423297858	24.495911631117245	20.8273032907334	26.56236084517077
43	28.19395121788307	24.29872923809948	20.64953299639311	26.857786547624347
44	28.34034209823218	24.063279267043683	20.93431335441065	26.66206528031349
45	28.213641626218994	24.392937057487643	20.43495624972169	26.95846506657167
46	28.214546132608987	24.117480073028457	20.853673130872334	26.814300663490226
47	28.96472981698357	24.42389900699114	20.083451151088745	26.527920024936545
48	28.868225942913124	24.880953043594424	20.083729460747204	26.167091552745248
49	28.78682036781404	24.84637306853097	19.977136861557643	26.38966970209734
50	29.057754820323282	24.92617836309391	19.683798481542503	26.332268335040297
51	28.895639444271275	25.42149997773523	19.715734514850602	25.9671260631429
52	28.88729015451752	25.045781938816404	19.664386382865032	26.402541523801045
53	28.791551632007838	25.379683951551858	19.45537582936278	26.373388587077528
54	28.703953667008058	25.79179097831411	19.465882018969584	26.038373335708243
55	28.614268379569847	25.64039052411275	19.242747250300575	26.50259384601683
56	28.77255699781805	25.929554259251013	19.123143674578085	26.174745068352852
57	28.641821035757225	26.039138687269002	19.114237765507415	26.204802511466358
58	28.291777062831187	26.135294674266373	19.082232154784702	26.490696108117735
59	28.433436678986507	26.24703600213742	18.861184708554127	26.45834261032195
60	28.25462272342699	26.594923075210403	18.8849106069377	26.2655435944249
61	28.379792492318657	26.328928619138797	18.725230440397205	26.566048448145345
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	2.0
2	3.0
3	2.0
4	2.0
5	2.0
6	2.5
7	5.0
8	7.0
9	7.5
10	8.0
11	11.0
12	14.5
13	15.0
14	23.5
15	32.0
16	47.5
17	85.5
18	108.0
19	150.0
20	244.0
21	296.0
22	383.0
23	470.0
24	605.5
25	960.5
26	1180.0
27	1582.5
28	1985.0
29	2452.0
30	3573.5
31	4228.0
32	5191.0
33	6154.0
34	7129.0
35	9655.0
36	11206.0
37	13109.0
38	15012.0
39	17331.0
40	22834.5
41	26019.0
42	29319.0
43	36695.5
44	40772.0
45	45680.5
46	50589.0
47	55265.5
48	64941.0
49	69940.0
50	75639.5
51	81339.0
52	86473.0
53	95858.5
54	100110.0
55	103083.5
56	106057.0
57	106730.0
58	105984.5
59	104566.0
60	101770.5
61	97008.5
62	95042.0
63	85850.5
64	76659.0
65	69287.5
66	55710.5
67	49505.0
68	43736.0
69	37967.0
70	32418.5
71	22745.0
72	18620.0
73	15256.0
74	11892.0
75	9666.5
76	5758.0
77	4075.0
78	3210.0
79	2345.0
80	1798.0
81	953.0
82	655.0
83	507.5
84	260.5
85	161.0
86	114.5
87	68.0
88	49.5
89	25.0
90	19.0
91	16.0
92	13.0
93	10.5
94	7.5
95	7.0
96	5.5
97	4.0
98	3.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03339715901500646
3	0.0
4	2.0873224384379034E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	6.957741461459678E-5
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.110180010687091
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1437248.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.18010919020398
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.80203492055907	59.197236640075424
2	14.891901638751904	22.09365779224787
3	3.326615377206095	7.403060757448792
4	0.9110482683497869	2.7032664009493375
5	0.3427131731325528	1.2711250301947024
6	0.16819330908395655	0.7485958819745773
7	0.11212035590345609	0.5821970170354033
8	0.07205224262972687	0.4275874580537767
9	0.05465132458685522	0.3648637102717979
>10	0.30870272482284256	4.176433504814099
>50	0.008832440767917888	0.3892465666780289
>100	0.0010311129143974573	0.11656781651200764
>500	0.0	0.0
>1k	0.0	0.0
>5k	1.0311129143974573E-4	0.5261614237441604
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTGGGGGGGGGGGGGGG	6879	0.47862303513381127	TruSeq Adapter, Index 11 (97% over 39bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.5317801798993632	0.0	0.0	0.0	0.0
2	0.53233679921628	0.0	0.0	0.0	0.0
3	0.5331717281916551	0.0	0.0	0.0	6.957741461459678E-5
4	0.5336587700939573	0.0	0.0	0.0	6.957741461459678E-5
5	0.5348415861424055	0.0	0.0	0.0	6.957741461459678E-5
6	0.5372767956539164	0.0	0.0	0.0	6.957741461459678E-5
7	0.5400598922385003	0.0	0.0	0.0	6.957741461459678E-5
8	0.5628812842320879	0.0	0.0	0.0	6.957741461459678E-5
9	0.5669167742797346	0.0	0.0	0.0	6.957741461459678E-5
10	0.5687257870597141	0.0	0.0	0.0	6.957741461459678E-5
11	0.5724133900342877	0.0	0.0	0.0	6.957741461459678E-5
12	0.5769359219842365	0.0	0.0	0.0	6.957741461459678E-5
13	0.585493943981832	0.0	0.0	0.0	6.957741461459678E-5
14	0.5961392884178652	0.0	0.0	0.0	6.957741461459678E-5
15	0.6113071648038474	0.0	0.0	0.0	6.957741461459678E-5
16	0.6303713764082469	0.0	0.0	0.0	1.3915482922919356E-4
17	0.6485310816226566	0.0	0.0	0.0	1.3915482922919356E-4
18	0.6717699381039319	0.0	0.0	0.0	1.3915482922919356E-4
19	0.7055149841920114	0.0	0.0	0.0	1.3915482922919356E-4
20	0.7462873491561651	0.0	0.0	0.0	2.783096584583871E-4
21	0.8596985349779579	0.0	0.0	0.0	2.783096584583871E-4
22	0.9278844013002627	0.0	0.0	0.0	3.4788707307298393E-4
23	1.01896123703077	0.0	0.0	0.0	4.1746448768758073E-4
24	1.1381473482655742	0.0	0.0	0.0	4.1746448768758073E-4
25	1.2782066838847574	0.0	0.0	0.0	4.1746448768758073E-4
26	1.4602907779311574	0.0	6.957741461459678E-5	0.0	4.870419023021775E-4
27	1.6762590728948656	0.0	6.957741461459678E-5	0.0	4.870419023021775E-4
28	1.9199887562897984	0.0	6.957741461459678E-5	0.0	4.870419023021775E-4
29	2.1983679921628	0.0	6.957741461459678E-5	0.0	6.957741461459679E-4
30	2.532130850069021	0.0	6.957741461459678E-5	0.0	6.957741461459679E-4
31	2.9008911475263837	0.0	6.957741461459678E-5	0.0	6.957741461459679E-4
32	3.311397893752505	0.0	6.957741461459678E-5	0.0	6.957741461459679E-4
33	3.774018123524959	0.0	6.957741461459678E-5	0.0	6.957741461459679E-4
34	4.292856904306007	0.0	6.957741461459678E-5	0.0	6.957741461459679E-4
35	4.847180166540499	0.0	6.957741461459678E-5	0.0	6.957741461459679E-4
36	5.468158591975776	0.0	6.957741461459678E-5	0.0	6.957741461459679E-4
37	6.120725163646079	0.0	6.957741461459678E-5	0.0	6.957741461459679E-4
38	6.832571692568019	0.0	6.957741461459678E-5	0.0	6.957741461459679E-4
39	7.596114240548604	0.0	1.3915482922919356E-4	0.0	7.653515607605647E-4
40	8.457273901233469	0.0	1.3915482922919356E-4	0.0	7.653515607605647E-4
41	9.385227879948346	0.0	1.3915482922919356E-4	0.0	7.653515607605647E-4
42	10.351310281872022	0.0	1.3915482922919356E-4	0.0	7.653515607605647E-4
43	11.387874604800285	0.0	1.3915482922919356E-4	0.0	7.653515607605647E-4
44	12.419498931290912	0.0	1.3915482922919356E-4	0.0	7.653515607605647E-4
45	13.527171371955292	0.0	1.3915482922919356E-4	0.0	7.653515607605647E-4
46	14.658569711003251	0.0	1.3915482922919356E-4	0.0	7.653515607605647E-4
47	15.818912254530881	0.0	1.3915482922919356E-4	0.0	7.653515607605647E-4
48	17.0149480117558	0.0	1.3915482922919356E-4	0.0	7.653515607605647E-4
49	18.265741194282406	6.957741461459678E-5	1.3915482922919356E-4	0.0	7.653515607605647E-4
50	19.526136060025827	6.957741461459678E-5	1.3915482922919356E-4	0.0	7.653515607605647E-4
>>END_MODULE