##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-35B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 739259 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.58909664948279 40.0 40.0 40.0 40.0 40.0 2 39.35851710970039 40.0 40.0 40.0 40.0 40.0 3 39.32848433363679 40.0 40.0 40.0 40.0 40.0 4 39.22517277435919 40.0 40.0 40.0 40.0 40.0 5 39.26022138384518 40.0 40.0 40.0 40.0 40.0 6 39.375347476324265 40.0 40.0 40.0 40.0 40.0 7 39.40852123545334 40.0 40.0 40.0 40.0 40.0 8 39.42998056161643 40.0 40.0 40.0 40.0 40.0 9 39.43233968068025 40.0 40.0 40.0 40.0 40.0 10-11 39.4372121272788 40.0 40.0 40.0 40.0 40.0 12-13 39.35909877323104 40.0 40.0 40.0 40.0 40.0 14-15 39.39842463872608 40.0 40.0 40.0 40.0 40.0 16-17 39.495432588578566 40.0 40.0 40.0 40.0 40.0 18-19 39.53269693030454 40.0 40.0 40.0 40.0 40.0 20-21 39.513004238027534 40.0 40.0 40.0 40.0 40.0 22-23 39.55162128563873 40.0 40.0 40.0 40.0 40.0 24-25 39.536295127959214 40.0 40.0 40.0 40.0 40.0 26-27 39.51594231521023 40.0 40.0 40.0 40.0 40.0 28-29 39.52528139664177 40.0 40.0 40.0 40.0 40.0 30-31 39.48643833665388 40.0 40.0 40.0 40.0 40.0 32-33 39.48372639813385 40.0 40.0 40.0 40.0 40.0 34-35 39.4746512236853 40.0 40.0 40.0 40.0 40.0 36-37 39.45380289578175 40.0 40.0 40.0 40.0 40.0 38-39 39.386628641046286 40.0 40.0 40.0 40.0 40.0 40-41 39.42925198382157 40.0 40.0 40.0 40.0 40.0 42-43 39.406759876189064 40.0 40.0 40.0 40.0 40.0 44-45 39.328279005942875 40.0 40.0 40.0 40.0 40.0 46-47 39.282802359791575 40.0 40.0 40.0 40.0 40.0 48-49 39.34435507099695 40.0 40.0 40.0 40.0 40.0 50-51 39.287632125383986 40.0 40.0 40.0 40.0 40.0 52-53 39.231261699631006 40.0 40.0 40.0 40.0 40.0 54-55 39.123348851663565 40.0 40.0 40.0 40.0 40.0 56-57 39.13577340484477 40.0 40.0 40.0 40.0 40.0 58-59 39.08792995137004 40.0 40.0 40.0 40.0 40.0 60-61 39.002525298798815 40.0 40.0 40.0 40.0 40.0 62-63 39.39660274114972 40.0 40.0 40.0 40.0 40.0 64-65 39.40532260706016 40.0 40.0 40.0 40.0 40.0 66-67 39.40853455888921 40.0 40.0 40.0 40.0 40.0 68-69 39.40070922835782 40.0 40.0 40.0 40.0 40.0 70-71 39.405093046556914 40.0 40.0 40.0 40.0 40.0 72-73 39.4068245890113 40.0 40.0 40.0 40.0 40.0 74-75 39.40131600359259 40.0 40.0 40.0 40.0 40.0 76-77 39.40299433987609 40.0 40.0 40.0 40.0 40.0 78-79 39.407346932421405 40.0 40.0 40.0 40.0 40.0 80-81 39.406656232852114 40.0 40.0 40.0 40.0 40.0 82-83 39.40460643290382 40.0 40.0 40.0 40.0 40.0 84-85 39.37338694031746 40.0 40.0 40.0 40.0 40.0 86-87 39.37903329175591 40.0 40.0 40.0 40.0 40.0 88-89 39.41920325969553 40.0 40.0 40.0 40.0 40.0 90-91 39.501943834206024 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09223869869695278 1101 2 0.0027792364474450437 1101 3 -0.0029543832538863057 1101 4 0.05879259176332141 1101 5 -0.10992890646424769 1101 6 -0.11450079870564167 1101 7 0.009006528866208896 1101 8 -0.0031471746036046966 1101 9 -0.06191506862916185 1101 10-11 -0.054139284357489714 1101 12-13 -0.05496706988441957 1101 14-15 -0.018961654936518357 1101 16-17 0.020077538727001354 1101 18-19 0.04852399178010813 1101 20-21 0.08926436364919255 1101 22-23 0.08300428789951297 1101 24-25 0.007766327632801051 1101 26-27 -0.0536419002929307 1101 28-29 0.002594056117054322 1101 30-31 -8.391860039580479E-4 1101 32-33 0.051479108334746115 1101 34-35 0.041097590922952065 1101 36-37 -0.03257513748579299 1101 38-39 -0.0019820676678818927 1101 40-41 0.03857100877952746 1101 42-43 -0.054096617276528036 1101 44-45 0.026592108636798173 1101 46-47 0.0635095412120279 1101 48-49 -0.06165631774744895 1101 50-51 -0.04674503639333949 1101 52-53 0.047632699130375045 1101 54-55 -0.049697314358240874 1101 56-57 0.021344743562849544 1101 58-59 0.04245574287589449 1101 60-61 0.0883257125559993 1101 62-63 -0.06976355256058753 1101 64-65 -6.951870752942568E-4 1101 66-67 -0.02798801503124082 1101 68-69 -0.1426749370870226 1101 70-71 0.08406752040970389 1101 72-73 -0.06253243486941784 1101 74-75 -0.0073816986831616305 1101 76-77 -0.035251737740487954 1101 78-79 0.03083408878256222 1101 80-81 0.16092294073443014 1101 82-83 0.07245576235673212 1101 84-85 0.17371553469310896 1101 86-87 0.2394543299809868 1101 88-89 0.18856488944364713 1101 90-91 0.4613771958887938 1102 1 0.0969068228137786 1102 2 -0.2341553450506879 1102 3 -0.038344210374191334 1102 4 -0.1558034298771105 1102 5 -0.20934760925202056 1102 6 -0.04849941876857855 1102 7 -0.15383624347120417 1102 8 -0.21060383228997637 1102 9 -0.11785504493667531 1102 10-11 -0.09577401511933203 1102 12-13 -0.09373891570067627 1102 14-15 -0.08300327946819408 1102 16-17 -0.04820843767535621 1102 18-19 -0.11103161341102918 1102 20-21 -0.0826520303192666 1102 22-23 -0.21279791908186496 1102 24-25 -0.01426829930228024 1102 26-27 0.017876168162352712 1102 28-29 -0.05896204984946252 1102 30-31 -0.013544194114786023 1102 32-33 -0.03478189163879364 1102 34-35 -0.05609595502141218 1102 36-37 -0.043721099567370914 1102 38-39 0.009396273409798539 1102 40-41 0.030109480860765814 1102 42-43 -0.06320030254497055 1102 44-45 -0.03240272884715978 1102 46-47 -0.1514631873088632 1102 48-49 -0.06840356238983958 1102 50-51 0.011329448222902272 1102 52-53 0.013843231675039647 1102 54-55 -0.07347257211445424 1102 56-57 -0.0013381656505089268 1102 58-59 0.17982090895026914 1102 60-61 -0.06471258393553114 1102 62-63 -0.049950616051638974 1102 64-65 -0.052212438322179366 1102 66-67 -0.19270891752037045 1102 68-69 -0.07664136191429094 1102 70-71 -0.02482912436062179 1102 72-73 0.07308781355051508 1102 74-75 -0.03828240919909831 1102 76-77 0.05787741223956999 1102 78-79 -0.05583670186543088 1102 80-81 -0.23347263542520125 1102 82-83 0.13869978026826146 1102 84-85 0.1851376544647394 1102 86-87 -0.3075302243181568 1102 88-89 0.1768186524873272 1102 90-91 -0.1663151118035131 1103 1 -0.047373983487936755 1103 2 0.22884158832951584 1103 3 -0.01793952794860587 1103 4 0.006689129827996965 1103 5 -0.028803414770543156 1103 6 0.20474559405831627 1103 7 0.039976912014424215 1103 8 0.13855919979988585 1103 9 0.11357902621865179 1103 10-11 0.11260297374546724 1103 12-13 0.04524405255057928 1103 14-15 0.04192057716298336 1103 16-17 0.0871544572694134 1103 18-19 -0.003445819414082507 1103 20-21 -0.004949245299414429 1103 22-23 -0.07509589984674392 1103 24-25 -0.05240089841048956 1103 26-27 -0.03425288745102506 1103 28-29 -0.02620386726549384 1103 30-31 -0.01764462330232419 1103 32-33 0.02936925590518058 1103 34-35 -0.022704580834066235 1103 36-37 0.04486238041310031 1103 38-39 0.03975525779399902 1103 40-41 -0.028480473651704585 1103 42-43 -0.03771574465062599 1103 44-45 0.05339094578771153 1103 46-47 -0.07484856608959944 1103 48-49 -0.06892187793066995 1103 50-51 -0.0245477454162355 1103 52-53 0.11636919273874469 1103 54-55 -0.002708246240580081 1103 56-57 0.06188267243474854 1103 58-59 -0.10797311265024945 1103 60-61 0.09331414900385937 1103 62-63 -0.14445383415337432 1103 64-65 0.035359138323343586 1103 66-67 -0.008952362971022865 1103 68-69 0.023236684395278928 1103 70-71 -0.0169822070779162 1103 72-73 -0.0336853766164964 1103 74-75 0.05122221107379232 1103 76-77 -0.09976391544719831 1103 78-79 -0.04633178972603247 1103 80-81 -0.042727918192767333 1103 82-83 0.0674268402971947 1103 84-85 0.04763733864349007 1103 86-87 0.07592115776022723 1103 88-89 0.08072681395473325 1103 90-91 0.26137719588879094 1104 1 0.0891395900465426 1104 2 0.14019530429995797 1104 3 0.059557887527908804 1104 4 0.11142933735565919 1104 5 0.05930873440432549 1104 6 0.1347423394731777 1104 7 -0.009680399315357135 1104 8 0.04946110277496274 1104 9 0.0913607392791107 1104 10-11 0.0030646462193288926 1104 12-13 -0.017714939676707786 1104 14-15 0.005171046206136509 1104 16-17 0.034458894991978184 1104 18-19 -0.03596917584859227 1104 20-21 -0.003930751597991389 1104 22-23 -0.021576640360585486 1104 24-25 0.042362569828590324 1104 26-27 0.07483171261789323 1104 28-29 0.049916571529159626 1104 30-31 0.017187574116981352 1104 32-33 -0.023779230191188105 1104 34-35 0.05423510213348237 1104 36-37 -0.02055503586317542 1104 38-39 -0.03698641168411143 1104 40-41 -0.013901086754614767 1104 42-43 -0.04777861237045755 1104 44-45 -0.08057342016496705 1104 46-47 -0.06797981424197985 1104 48-49 -0.009425919837660501 1104 50-51 0.13797553393875006 1104 52-53 -0.0422783477095976 1104 54-55 -0.1142663494409959 1104 56-57 -0.024749354891667963 1104 58-59 0.012761642773305937 1104 60-61 0.06995459543767879 1104 62-63 -0.08236101482186342 1104 64-65 -0.02305666489746727 1104 66-67 -0.1210867668036002 1104 68-69 -0.07568129266143586 1104 70-71 -0.03466502539571081 1104 72-73 -0.009555022414183156 1104 74-75 -0.20471896412488633 1104 76-77 -0.10782798087906542 1104 78-79 -0.04115849230071689 1104 80-81 -0.10860938139618526 1104 82-83 0.06087156138774219 1104 84-85 -0.3866744618084752 1104 86-87 -0.4788734293035546 1104 88-89 -0.8361921716770482 1104 90-91 -1.27331668166223 1201 1 -0.007097040715073888 1201 2 0.062095163470971215 1201 3 0.06336407639099662 1201 4 0.06661146349275526 1201 5 0.08863446804723196 1201 6 0.03905379990784752 1201 7 0.21953210020886615 1201 8 0.1391453794087667 1201 9 0.0451526549339647 1201 10-11 0.01237315685762752 1201 12-13 -0.02141836144048881 1201 14-15 0.046385886536512544 1201 16-17 -0.011243917781598611 1201 18-19 0.0291152263894503 1201 20-21 0.08020885940950961 1201 22-23 0.05469510249329801 1201 24-25 -0.053256031016381655 1201 26-27 0.07571046647165502 1201 28-29 0.031262915684756365 1201 30-31 0.030103400250276025 1201 32-33 0.005864707708099104 1201 34-35 0.08471281328545643 1201 36-37 0.029542812737659574 1201 38-39 0.06426687564012212 1201 40-41 0.025365018955405105 1201 42-43 0.0028043009797897867 1201 44-45 6.081376212705436E-4 1201 46-47 0.008684670647404857 1201 48-49 -0.018302772078605756 1201 50-51 0.017050680081062808 1201 52-53 -0.021641916426602847 1201 54-55 -0.09541389582196302 1201 56-57 -0.05735429983474205 1201 58-59 0.05751278090311018 1201 60-61 -0.016553093644397165 1201 62-63 0.018973806171999286 1201 64-65 -0.12287875496936351 1201 66-67 0.011162936831702552 1201 68-69 -0.12189903816290837 1201 70-71 -0.046779950046698104 1201 72-73 -0.056825197740089095 1201 74-75 -0.22534154400649697 1201 76-77 0.04446455439074981 1201 78-79 0.02205910979144221 1201 80-81 -0.259179069792161 1201 82-83 -0.020564179494336088 1201 84-85 -0.17741721827078294 1201 86-87 0.13611750662054334 1201 88-89 -0.08239970152094855 1201 90-91 -0.4623700807996656 1202 1 0.07187705805798572 1202 2 -0.01744055299343472 1202 3 0.027192992149309703 1202 4 0.06860230137793621 1202 5 0.14303972943669407 1202 6 0.033993430847559125 1202 7 -0.04805161713863271 1202 8 0.03832532074876838 1202 9 -0.0015603806968016443 1202 10-11 0.002942337021679009 1202 12-13 0.08889352035641451 1202 14-15 0.05096549148688467 1202 16-17 0.03577607096668345 1202 18-19 0.04495623435764173 1202 20-21 0.07714707468531401 1202 22-23 0.022945789264582572 1202 24-25 -0.003334028094037933 1202 26-27 0.06075110144823981 1202 28-29 -0.0732149202667145 1202 30-31 0.0434878149720177 1202 32-33 -0.0640950966576952 1202 34-35 0.11137613229045229 1202 36-37 -0.2902509715410062 1202 38-39 -0.1428905868917667 1202 40-41 -0.1465972099579531 1202 42-43 -0.14860344800140268 1202 44-45 -0.0848909782496321 1202 46-47 -0.07007902620827622 1202 48-49 -0.030263594451689357 1202 50-51 -0.08818311233199694 1202 52-53 -0.05501929063498068 1202 54-55 -0.001767767611440263 1202 56-57 -0.043741510322156785 1202 58-59 -0.2610088980138059 1202 60-61 -0.21141618005061247 1202 62-63 -0.07933853171703475 1202 64-65 -0.16274486257451315 1202 66-67 -0.002354960629659786 1202 68-69 -0.069852135292642 1202 70-71 0.035959912699034646 1202 72-73 -0.09450153416799623 1202 74-75 -0.16985720434882978 1202 76-77 -0.0717793681415202 1202 78-79 -0.02880639587058198 1202 80-81 -0.20514048120322315 1202 82-83 -0.35193792914218136 1202 84-85 -0.20671965642256396 1202 86-87 0.03670584471962712 1202 88-89 -0.08176478088602579 1202 90-91 -0.6087982427076994 1203 1 0.06557657386195359 1203 2 0.15872332178079063 1203 3 -0.03184613454534713 1203 4 0.02189561638235915 1203 5 0.10068035027040168 1203 6 -0.03352642835023545 1203 7 0.0664538179358587 1203 8 0.18544402933323312 1203 9 0.003977864570764211 1203 10-11 0.10239661540565237 1203 12-13 0.01213210302541512 1203 14-15 -0.06409536985253084 1203 16-17 0.057837658741462405 1203 18-19 -0.02130814477566645 1203 20-21 0.033853034187721676 1203 22-23 0.005666332121919027 1203 24-25 0.15055532194887888 1203 26-27 0.009722549994286567 1203 28-29 0.022325463792405742 1203 30-31 0.02415907517249849 1203 32-33 0.01107301977398123 1203 34-35 0.02075426632361399 1203 36-37 0.011075652691218352 1203 38-39 -0.003941917931626904 1203 40-41 0.03791047217497834 1203 42-43 -0.08185254428818212 1203 44-45 0.03398892125086661 1203 46-47 0.07599993405239758 1203 48-49 -0.036453616131446154 1203 50-51 0.024114892616147188 1203 52-53 0.03125895279313795 1203 54-55 0.04800106819108407 1203 56-57 0.02897660621754028 1203 58-59 0.053406027004122336 1203 60-61 0.0656028394561261 1203 62-63 0.15986102579008588 1203 64-65 0.05419208514341278 1203 66-67 0.06323121712415514 1203 68-69 0.07125243420967564 1203 70-71 0.08100995720560888 1203 72-73 0.04175689437759189 1203 74-75 -0.01084436987495252 1203 76-77 -0.054611324860964316 1203 78-79 0.0697372975060162 1203 80-81 0.12039565943057084 1203 82-83 -0.018375886328399815 1203 84-85 0.39933837878789546 1203 86-87 0.33016711351053374 1203 88-89 0.23352193187039205 1203 90-91 0.3280438625554609 1204 1 0.03498208088903709 1204 2 0.1145359636406198 1204 3 0.013990121960141266 1204 4 -0.25948641356009006 1204 5 -0.16078284068724713 1204 6 0.006152595883435197 1204 7 -0.06469871433367302 1204 8 -0.10938505107119312 1204 9 -0.15722900431063636 1204 10-11 -0.07634011568543286 1204 12-13 -0.10943655139831066 1204 14-15 -0.21271356917848294 1204 16-17 -0.0819147313816515 1204 18-19 -0.07009921247862394 1204 20-21 -0.01623844390567797 1204 22-23 0.03815779187384294 1204 24-25 -0.07832424335822452 1204 26-27 -0.035543745257562875 1204 28-29 -0.07926841015582653 1204 30-31 0.07248977191918016 1204 32-33 -0.04494782577253176 1204 34-35 -0.03950123292595009 1204 36-37 -0.01564191305160989 1204 38-39 0.06369279975259445 1204 40-41 -0.020262735334831916 1204 42-43 -0.04157646894186939 1204 44-45 -0.061554833292220223 1204 46-47 0.032182907096490965 1204 48-49 -0.09654405415756884 1204 50-51 -0.03726829146496158 1204 52-53 -0.036723126509627946 1204 54-55 0.056361346348943187 1204 56-57 -0.03906332081767516 1204 58-59 -0.05325733235593333 1204 60-61 -0.1871443690699266 1204 62-63 0.02805007067615861 1204 64-65 0.01021418806659824 1204 66-67 0.00969815806827512 1204 68-69 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0.004206069456380135 2401 30-31 -0.04718944108338263 2401 32-33 0.08480022148976474 2401 34-35 -0.060036877983549175 2401 36-37 0.040592764296839334 2401 38-39 0.13264844563825307 2401 40-41 1.532178170080556E-4 2401 42-43 0.13318640871496257 2401 44-45 0.0880432238195894 2401 46-47 0.03487505772422139 2401 48-49 -0.07164943133238211 2401 50-51 0.07386671028194058 2401 52-53 0.06532860788340145 2401 54-55 0.13145999348500936 2401 56-57 -0.08681853599897238 2401 58-59 -0.10763762529722243 2401 60-61 -0.07008991798136321 2401 62-63 -0.013324456574224541 2401 64-65 0.01568929578574796 2401 66-67 -0.021956880125458156 2401 68-69 0.04166683093708201 2401 70-71 0.0721206760420543 2401 72-73 0.05048968810721277 2401 74-75 0.06429045200007977 2401 76-77 0.10663237888455512 2401 78-79 -0.1363256711414209 2401 80-81 0.09422791233868821 2401 82-83 0.0627882710530443 2401 84-85 0.06924718370333238 2401 86-87 -0.0019144593374917918 2401 88-89 -0.0175459736208623 2401 90-91 0.13320382127579222 2402 1 -0.010585684678730445 2402 2 -0.11447502537036769 2402 3 0.08739671536673654 2402 4 0.031209557135881028 2402 5 0.09328309337868745 2402 6 0.05992548965632949 2402 7 -0.004918494553450614 2402 8 0.0050471833610430394 2402 9 0.11031678323515592 2402 10-11 0.06446574512042957 2402 12-13 0.058841836880070275 2402 14-15 0.04999705353214523 2402 16-17 0.07786548839856522 2402 18-19 0.04008393770452301 2402 20-21 0.055409907742671294 2402 22-23 0.07251676623282322 2402 24-25 0.03288454785056416 2402 26-27 -0.035470485184298184 2402 28-29 -0.028059618947033016 2402 30-31 -0.0013563819269748478 2402 32-33 0.05528705633511066 2402 34-35 0.1419824628537043 2402 36-37 0.060917734931933865 2402 38-39 -0.4344448455610177 2402 40-41 0.005303789391817304 2402 42-43 0.09770408349197623 2402 44-45 0.067599714252367 2402 46-47 0.035734089099825894 2402 48-49 0.05882588616673701 2402 50-51 -0.030479411872512685 2402 52-53 0.0464011560158184 2402 54-55 0.2777549834555515 2402 56-57 0.037811304305265026 2402 58-59 0.16376211485580683 2402 60-61 0.1268963915070529 2402 62-63 0.1530249869807463 2402 64-65 0.12435823925477507 2402 66-67 0.17671525951247702 2402 68-69 0.01850384659005755 2402 70-71 0.15204839136806925 2402 72-73 0.03286600793396843 2402 74-75 0.3270794131761008 2402 76-77 0.2581308570309986 2402 78-79 0.07176802944203331 2402 80-81 0.3258661621939041 2402 82-83 0.07967281224134837 2402 84-85 0.33375825768923306 2402 86-87 0.2542037744765864 2402 88-89 0.2692556272772393 2402 90-91 0.2508508800993212 2403 1 -0.04248767178187762 2403 2 -0.026018067322979732 2403 3 0.12981745760625074 2403 4 -0.004568655228055718 2403 5 0.01319461836451552 2403 6 0.03550139375705896 2403 7 0.04362486736432203 2403 8 0.05448591667074254 2403 9 0.006617800087092007 2403 10-11 0.07553202570464634 2403 12-13 0.04767385782518119 2403 14-15 0.1321795080234054 2403 16-17 0.028820006877545268 2403 18-19 0.06645889173442754 2403 20-21 0.023152639820310128 2403 22-23 0.033721056686843554 2403 24-25 0.03820659079493538 2403 26-27 0.0983118615012657 2403 28-29 0.05417780051401877 2403 30-31 0.013588513699325233 2403 32-33 0.02183809200625575 2403 34-35 0.004669326019069331 2403 36-37 0.0407447696111376 2403 38-39 0.1522081261469097 2403 40-41 0.056826937854317805 2403 42-43 0.18995019188463402 2403 44-45 0.08288622607446428 2403 46-47 0.04992473016072552 2403 48-49 0.14921872428929106 2403 50-51 0.14890686087228744 2403 52-53 0.13097749010091775 2403 54-55 0.07604873078983587 2403 56-57 0.1647439754104454 2403 58-59 0.024839810342051294 2403 60-61 0.11526594263305867 2403 62-63 0.161474443038216 2403 64-65 0.14014707116488978 2403 66-67 0.15466837155854307 2403 68-69 0.15285501505046284 2403 70-71 0.13307650069812382 2403 72-73 0.22485874838034903 2403 74-75 0.14635548180716995 2403 76-77 0.17774827891487632 2403 78-79 0.20204860135675062 2403 80-81 0.1266772186071492 2403 82-83 0.20908089061689594 2403 84-85 0.1251809610065706 2403 86-87 -0.18521136210588196 2403 88-89 -0.057308007885559675 2403 90-91 -0.020506862082221744 2404 1 0.039609281645269334 2404 2 -0.016211998075085887 2404 3 0.041124707804407024 2404 4 0.05772497719968328 2404 5 -0.047187189027077636 2404 6 0.014598773361868211 2404 7 -0.007659839230278465 2404 8 -0.01499299151784328 2404 9 0.053457513472658036 2404 10-11 0.05581633001939679 2404 12-13 -0.011369672041112722 2404 14-15 -0.005113427384792146 2404 16-17 -0.03209551833663227 2404 18-19 0.041525506888028474 2404 20-21 0.014573324970598378 2404 22-23 0.06131506470853765 2404 24-25 0.03564952480909511 2404 26-27 -0.010869279937935517 2404 28-29 -0.027799663848362854 2404 30-31 0.057417102652962626 2404 32-33 -0.06649732085557503 2404 34-35 0.01850031788627149 2404 36-37 0.07595397497067324 2404 38-39 0.09232720244070691 2404 40-41 0.055696912478580884 2404 42-43 0.11186254421498631 2404 44-45 -0.0481415627496915 2404 46-47 0.036787732379295335 2404 48-49 0.13617644405663754 2404 50-51 0.0548515165034118 2404 52-53 0.05128558131768557 2404 54-55 0.09400719349709163 2404 56-57 0.016508810226575577 2404 58-59 0.02970564576900614 2404 60-61 -0.029307628727053725 2404 62-63 0.0464068971803826 2404 64-65 0.1484928400194434 2404 66-67 0.04427189677890908 2404 68-69 0.11029639965439486 2404 70-71 0.027737418332520747 2404 72-73 0.08548696612635354 2404 74-75 0.11521194625837694 2404 76-77 0.024155161717573037 2404 78-79 0.026165437642262646 2404 80-81 -0.054271533814393536 2404 82-83 -0.0576089776810349 2404 84-85 -0.07899819297335853 2404 86-87 -0.22988165106155378 2404 88-89 -0.7319495950842665 2404 90-91 -0.18555726499915437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 2.0 30 18.0 31 473.0 32 2925.0 33 5172.0 34 6880.0 35 8645.0 36 12627.0 37 19008.0 38 51260.0 39 354704.0 40 277545.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 30.974124089121673 13.522053840399643 23.201611343250473 32.30221072722821 2 30.573243236660723 17.19269664897748 23.870792278819795 28.363267835542 3 27.7531961058303 18.98671507550128 19.949300583422048 33.31078823524638 4 27.87321882211623 20.190650625356778 19.41947195701636 32.516658595510634 5 28.331613142349298 20.50296310224157 19.501825476592103 31.66359827881703 6 28.443481919056783 22.929852730910277 22.186811388160308 26.439853961872632 7 27.354824222633745 22.589511930189555 21.69145049299366 28.364213354183036 8 26.577965232753336 23.32132581409222 21.828750140343235 28.271958812811203 9 26.115339820008955 23.373675531850136 22.846120236615313 27.664864411525596 10-11 26.578506315107425 22.069869964383255 22.333512341412142 29.018111379097178 12-13 26.774107586109874 21.930000175851767 22.285423647192662 29.0104685908457 14-15 27.218065657638256 21.69246502240757 22.11187148211926 28.977597837834914 16-17 27.46818097581497 21.51728961027191 21.679343775320962 29.335185638592158 18-19 27.387965516821573 21.86080926982289 21.823745128567932 28.927480084787604 20-21 27.31654264608209 21.854045740396803 21.896723611075412 28.932688002445694 22-23 27.44173557575897 21.311475409836063 21.856345340401674 29.39044367400329 24-25 27.497196517052885 21.61103212811748 21.830576293288278 29.061195061541355 26-27 27.609876917291505 21.807783199122362 21.793917963798883 28.788421919787243 28-29 27.71687595281221 21.561184916247214 21.77897056376723 28.942968567173345 30-31 27.513435787554258 21.776324065920242 22.001144392274945 28.70909575425055 32-33 27.621567888490173 21.686957566621334 22.039180826601562 28.652293718286938 34-35 27.668672878437985 21.521264828270674 21.926850432338174 28.883211860953168 36-37 27.67241002101709 21.745420772339305 22.017697266366586 28.56447194027702 38-39 27.94875401711806 21.797576924880342 21.94502454454628 28.308644513455317 40-41 27.81763571349894 21.580059746244793 21.946210637670298 28.656093902585972 42-43 27.732280156423865 21.802680029774727 22.136656249571875 28.328383564229533 44-45 27.935051110338893 21.901814059313466 22.003075582922023 28.16005924742561 46-47 27.9201587372727 21.574008808777812 22.024063201554778 28.481769252394706 48-49 27.869183302962185 21.882915189447292 22.082864481357145 28.16503702623338 50-51 28.031885247136362 21.919069387451298 22.041704953360384 28.007340412051956 52-53 28.097071839072445 21.679061367478944 22.000570704634328 28.22329608881428 54-55 27.985790206721738 21.89109351156627 22.0882558624702 28.03486041924179 56-57 28.21820173164124 21.954277867434442 21.987052102736858 27.84046829818746 58-59 28.171838098823798 21.75092993034217 21.909479051310957 28.16775291952307 60-61 28.37429040049536 21.92372957464207 21.918412009661424 27.783568015201144 62-63 28.399801549440905 22.088437710694706 21.90487094353064 27.606889796333757 64-65 28.426929600597443 21.689155766583674 21.801727570722512 28.08218706209637 66-67 28.34032985085619 22.01129963882639 21.80628889981077 27.842081610506654 68-69 28.535517969702877 22.02672730938695 21.811877555472552 27.62587716543762 70-71 28.577306256796074 21.853417874041202 21.683699709054576 27.88557616010815 72-73 28.415672210886456 22.043117129371996 21.765081720563078 27.77612893917847 74-75 28.63115609886277 21.95923011016485 21.717410540805005 27.69220325016738 76-77 28.724319803596238 21.774378481917633 21.47734477223369 28.023956942252436 78-79 28.704193665932603 22.11441879624202 21.3818343129644 27.79955322486098 80-81 28.880046347115503 21.97790889132821 21.13977314306623 28.00227161849006 82-83 29.046231131963694 21.541669628681785 20.976754105352953 28.435345134001565 84-85 29.31233809991901 21.581937669628008 20.800489641485985 28.305234588966993 86-87 29.87989420283571 20.84984607379786 19.98872653167411 29.281533191692322 88-89 28.884271997304733 20.506485484867202 20.521927115503395 30.087315402324666 90-91 25.272264631043257 23.331636980491943 22.490245971162 28.905852417302796 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 3.0 10 4.5 11 4.5 12 5.5 13 7.5 14 15.0 15 24.0 16 33.5 17 52.0 18 81.5 19 120.5 20 157.0 21 207.0 22 287.0 23 392.5 24 504.5 25 633.5 26 798.5 27 991.5 28 1246.5 29 1622.5 30 2024.0 31 2421.5 32 2906.0 33 3408.0 34 4098.0 35 5009.0 36 5992.0 37 7054.0 38 8377.5 39 9965.0 40 11574.0 41 13370.5 42 15518.0 43 17837.5 44 20254.5 45 22797.5 46 25704.0 47 28462.0 48 30795.0 49 33649.0 50 36558.5 51 39003.0 52 41046.5 53 43035.5 54 45047.5 55 46571.0 56 47855.5 57 48891.5 58 49581.5 59 50115.5 60 50100.5 61 49683.5 62 49031.0 63 47621.5 64 45337.0 65 42520.0 66 39104.0 67 35340.5 68 31513.5 69 27630.0 70 23836.0 71 20096.0 72 16387.0 73 12915.5 74 10054.5 75 7863.5 76 6048.0 77 4430.0 78 3241.5 79 2302.5 80 1589.5 81 1131.0 82 777.5 83 506.0 84 354.0 85 255.0 86 167.0 87 112.5 88 79.0 89 44.0 90 22.5 91 16.5 92 15.5 93 10.5 94 4.5 95 2.5 96 2.0 97 1.5 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.027189388292871645 3 0.0 4 1.3527058852174948E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.07316528145206053 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 1.2721173502813288E-4 64-65 0.0 66-67 0.0 68-69 1.65268768334504E-4 70-71 1.8367733036020963E-4 72-73 2.0610440012283821E-4 74-75 2.340939044288226E-4 76-77 2.6978465788607533E-4 78-79 0.0 80-81 0.0015245529248547863 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 4441.0 32-33 10394.0 34-35 12506.0 36-37 14321.0 38-39 16614.0 40-41 18930.0 42-43 20576.0 44-45 22137.0 46-47 23496.0 48-49 24987.0 50-51 26270.0 52-53 27075.0 54-55 28292.0 56-57 29053.0 58-59 29537.0 60-61 29989.0 62-63 30284.0 64-65 30021.0 66-67 30261.0 68-69 30325.0 70-71 29723.0 72-73 29250.0 74-75 28561.0 76-77 27689.0 78-79 26730.0 80-81 26057.0 82-83 24894.0 84-85 23527.0 86-87 22349.0 88-89 21196.0 90-91 19774.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.72045781296067 #Duplication Level Percentage of deduplicated Percentage of total 1 99.73764770230375 99.45883890061515 2 0.2472918870138663 0.49320120372907383 3 0.013047244427474191 0.03903231562515978 4 0.0015381253548453094 0.006135302582355874 5 2.6259068879460473E-4 0.0013092831852009332 6 0.0 0.0 7 2.1245021126013492E-4 0.0014829942630524605 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0020290588278262423 0.0 0.0 0.0 0.0 2 0.0020290588278262423 0.0 0.0 0.0 0.0 3 0.0021643294163479917 0.0 0.0 0.0 1.3527058852174948E-4 4 0.0021643294163479917 0.0 0.0 0.0 1.3527058852174948E-4 5 0.0021643294163479917 0.0 0.0 0.0 1.3527058852174948E-4 6 0.0021643294163479917 0.0 0.0 0.0 1.3527058852174948E-4 7 0.002299600004869741 0.0 0.0 0.0 1.3527058852174948E-4 8 0.007304611780174472 0.0 0.0 0.0 1.3527058852174948E-4 9 0.007575152957217971 0.0 0.0 0.0 1.3527058852174948E-4 10-11 0.007575152957217971 0.0 0.0 0.0 1.3527058852174948E-4 12-13 0.007575152957217971 0.0 0.0 0.0 1.3527058852174948E-4 14-15 0.007575152957217971 0.0 0.0 0.0 1.3527058852174948E-4 16-17 0.007575152957217971 0.0 0.0 0.0 2.7054117704349896E-4 18-19 0.007575152957217971 0.0 0.0 0.0 2.7054117704349896E-4 20-21 0.007778058840000594 0.0 0.0 0.0 2.7054117704349896E-4 22-23 0.008183870605565843 0.0 0.0 0.0 4.0581176556524846E-4 24-25 0.008319141194087592 0.0 0.0 0.0 4.0581176556524846E-4 26-27 0.008522047076870216 0.0 0.0 0.0 5.410823540869979E-4 28-29 0.008792588253913717 0.0 0.0 0.0 7.439882368696221E-4 30-31 0.009063129430957216 0.0 0.0 0.0 9.468941196522463E-4 32-33 0.00913076472521809 0.0 0.0 0.0 9.468941196522463E-4 34-35 0.009266035313739839 0.0 0.0 0.0 9.468941196522463E-4 36-37 0.009333670608000715 0.0 0.0 0.0 9.468941196522463E-4 38-39 0.009604211785044213 0.0 6.763529426087474E-5 0.0 0.0010145294139131211 40-41 0.009604211785044213 0.0 1.3527058852174948E-4 0.0 0.0010821647081739958 42-43 0.009604211785044213 0.0 1.3527058852174948E-4 0.0 0.0010821647081739958 44-45 0.009807117667826836 0.0 1.3527058852174948E-4 0.0 0.0010821647081739958 46-47 0.009942388256348588 0.0 1.3527058852174948E-4 0.0 0.0010821647081739958 48-49 0.010212929433392085 6.763529426087474E-5 1.3527058852174948E-4 0.0 0.0010821647081739958 50-51 0.01041583531617471 1.3527058852174948E-4 1.3527058852174948E-4 0.0 0.0011498000024348705 52-53 0.010686376493218208 1.3527058852174948E-4 1.3527058852174948E-4 0.0 0.0012850705909566201 54-55 0.010821647081739959 1.3527058852174948E-4 1.3527058852174948E-4 0.0 0.0014879764737392442 56-57 0.011092188258783458 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