##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-35A_S44_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	926571
Sequences flagged as poor quality	0
Sequence length	61
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.34614400839223	40.0	40.0	40.0	40.0	40.0
2	39.21164379200299	40.0	40.0	40.0	40.0	40.0
3	39.158113085775405	40.0	40.0	40.0	40.0	40.0
4	39.04341491369792	40.0	40.0	40.0	40.0	40.0
5	39.06640289842872	40.0	40.0	40.0	40.0	40.0
6	39.18495614475307	40.0	40.0	40.0	40.0	40.0
7	39.19798482793008	40.0	40.0	40.0	40.0	40.0
8	39.18530150414809	40.0	40.0	40.0	40.0	40.0
9	39.196327102834	40.0	40.0	40.0	40.0	40.0
10	39.22988308505231	40.0	40.0	40.0	40.0	40.0
11	39.20467616620853	40.0	40.0	40.0	40.0	40.0
12	39.2097443153304	40.0	40.0	40.0	40.0	40.0
13	39.0974938779651	40.0	40.0	40.0	40.0	40.0
14	39.235218887705315	40.0	40.0	40.0	40.0	40.0
15	39.18244797214677	40.0	40.0	40.0	40.0	40.0
16	39.197725808383815	40.0	40.0	40.0	40.0	40.0
17	39.232253113900605	40.0	40.0	40.0	40.0	40.0
18	39.18364809604445	40.0	40.0	40.0	40.0	40.0
19	39.24190159199889	40.0	40.0	40.0	40.0	40.0
20	38.85782740880084	40.0	40.0	40.0	40.0	40.0
21	39.103611056249335	40.0	40.0	40.0	40.0	40.0
22	39.09132489577161	40.0	40.0	40.0	40.0	40.0
23	39.21009399171785	40.0	40.0	40.0	40.0	40.0
24	39.21568449692468	40.0	40.0	40.0	40.0	40.0
25	38.98777751516074	40.0	40.0	40.0	40.0	40.0
26	39.16755866522911	40.0	40.0	40.0	40.0	40.0
27	39.171318765642354	40.0	40.0	40.0	40.0	40.0
28	38.93427702788021	40.0	40.0	40.0	40.0	40.0
29	39.1317017260415	40.0	40.0	40.0	40.0	40.0
30	38.80907993019423	40.0	40.0	40.0	40.0	40.0
31	38.97753760909849	40.0	40.0	40.0	40.0	40.0
32	39.021178085651286	40.0	40.0	40.0	40.0	40.0
33	39.03059560465415	40.0	40.0	40.0	40.0	40.0
34	38.969322372489536	40.0	40.0	40.0	40.0	40.0
35	38.86741113201255	40.0	40.0	40.0	40.0	40.0
36	38.95938033890549	40.0	40.0	40.0	40.0	40.0
37	38.93806303024809	40.0	40.0	40.0	40.0	40.0
38	38.87607533583503	40.0	40.0	40.0	40.0	40.0
39	38.919089848484354	40.0	40.0	40.0	40.0	40.0
40	38.69606106817503	40.0	40.0	40.0	40.0	40.0
41	38.76887146262941	40.0	40.0	40.0	40.0	40.0
42	38.56784639277508	40.0	40.0	40.0	40.0	40.0
43	38.8176966470999	40.0	40.0	40.0	40.0	40.0
44	38.42588209646104	40.0	40.0	40.0	40.0	40.0
45	38.81145427603497	40.0	40.0	40.0	40.0	40.0
46	38.48489538308451	40.0	40.0	40.0	40.0	40.0
47	38.00244557621596	40.0	40.0	40.0	40.0	40.0
48	38.44761383639246	40.0	40.0	40.0	40.0	40.0
49	38.12496182159813	40.0	40.0	40.0	40.0	40.0
50	38.474474163339885	40.0	40.0	40.0	40.0	40.0
51	38.176977263480076	40.0	40.0	40.0	40.0	40.0
52	38.2863223649348	40.0	40.0	40.0	40.0	40.0
53	37.6179353767817	40.0	40.0	40.0	24.0	40.0
54	37.03631130264168	40.0	40.0	40.0	24.0	40.0
55	37.0683692884841	40.0	40.0	40.0	24.0	40.0
56	36.96153235963569	40.0	40.0	40.0	24.0	40.0
57	37.09984016335499	40.0	40.0	40.0	24.0	40.0
58	37.188364410282645	40.0	40.0	40.0	24.0	40.0
59	36.811672284153076	40.0	40.0	40.0	24.0	40.0
60	36.32671862167066	40.0	40.0	40.0	24.0	40.0
61	36.24580954940312	40.0	40.0	40.0	24.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.015033930901545034
1101	2	0.0020357286560681587
1101	3	-0.008755486678708735
1101	4	-0.1002803688980265
1101	5	-0.06658302892576273
1101	6	0.054094292862956195
1101	7	0.020879678006139102
1101	8	0.08042410424332758
1101	9	-0.07753925273424045
1101	10	-0.03127152009680856
1101	11	0.00881963499694649
1101	12	0.025377280648648082
1101	13	-0.05326456054192619
1101	14	0.1294358454992235
1101	15	-0.020378468253582582
1101	16	-0.04930184620202027
1101	17	-0.009785282811527907
1101	18	-0.030788892604569185
1101	19	-0.020912346380839608
1101	20	0.08183385292964829
1101	21	0.0156454908510284
1101	22	-0.008750162710157383
1101	23	-0.034651465228257905
1101	24	0.025473893271133363
1101	25	-0.0811998643395242
1101	26	-0.18182470166433973
1101	27	-0.049093351613286984
1101	28	0.038864507708161966
1101	29	-0.05977112746291624
1101	30	-0.10636592974607595
1101	31	0.06902125686151805
1101	32	-0.05987678376303052
1101	33	-0.048624218469981884
1101	34	-0.034143990134332114
1101	35	0.018089410177545062
1101	36	-0.12479888905566838
1101	37	-0.1084717658298402
1101	38	0.06293712848930966
1101	39	-0.1040206092783933
1101	40	0.15193896562580278
1101	41	0.05304882746021633
1101	42	0.060639207447238164
1101	43	0.017055060750358564
1101	44	-0.05590964264241194
1101	45	0.0156362175918332
1101	46	-0.15625140508394963
1101	47	-0.1539955193352327
1101	48	0.17636232751308256
1101	49	0.20458495477631544
1101	50	0.06680914368683233
1101	51	0.09673649885728963
1101	52	0.08997502943712732
1101	53	0.06229984055791249
1101	54	0.08308399769527597
1101	55	0.19203347184487285
1101	56	0.26950705411965714
1101	57	0.05571806442390681
1101	58	0.5146463620529786
1101	59	0.14132846920403352
1101	60	0.10587783324454847
1101	61	0.23128363757481907
1102	1	-0.1284874723927132
1102	2	-0.16035311285383358
1102	3	-0.11733064125830595
1102	4	-0.01759871017324599
1102	5	-0.009951322224104331
1102	6	-0.12519814542051222
1102	7	-0.12598201926292774
1102	8	-0.10387312268413496
1102	9	-0.09696740091722234
1102	10	-0.12478256834409507
1102	11	-0.05276124153743211
1102	12	-0.07378925111602541
1102	13	-0.03361800678295168
1102	14	-0.03200250217894052
1102	15	-0.039931871808306596
1102	16	-0.05150127626746581
1102	17	-0.09727819480448119
1102	18	0.025617173553783346
1102	19	0.005127205376759036
1102	20	-0.04251805226333971
1102	21	-0.2394933707811262
1102	22	-0.10302404782429875
1102	23	-0.027842456608695443
1102	24	-0.15942383594393306
1102	25	0.028494901432928543
1102	26	0.035133880860861666
1102	27	-0.031173694116645834
1102	28	-0.11569112512086832
1102	29	0.0328694637786171
1102	30	-0.05784100392710201
1102	31	-0.10203778861115609
1102	32	-0.04388343631548253
1102	33	-0.026381215813849224
1102	34	-0.009955233454419954
1102	35	0.1430237302398396
1102	36	-0.13770742731191632
1102	37	-0.11065763033156628
1102	38	-0.016146942735325354
1102	39	0.05464584154508145
1102	40	0.07625849434003129
1102	41	-0.11669571488740615
1102	42	-0.11068120121989011
1102	43	-0.21163276035783696
1102	44	-0.1403720076989643
1102	45	0.01757925850285602
1102	46	-0.029050731508498018
1102	47	-0.3465085038406457
1102	48	0.17676703349757616
1102	49	0.12066069041422622
1102	50	-0.08221490444017121
1102	51	0.01923100706490999
1102	52	-0.06075014038112414
1102	53	0.10927648337029439
1102	54	-0.03491877148847067
1102	55	-0.12659720206519864
1102	56	-0.26118684255234825
1102	57	-0.15703700208805316
1102	58	-0.007002921097416959
1102	59	-0.03728116903855749
1102	60	0.11701199576542365
1102	61	-0.07565849444343797
1103	1	-0.0567344881676064
1103	2	-0.06769075740733399
1103	3	0.02994158975155159
1103	4	0.05583782043101593
1103	5	0.004853825156651226
1103	6	0.004370415835516894
1103	7	0.05007189148326319
1103	8	-0.07947718722947172
1103	9	0.03127928881465891
1103	10	-0.022480518371949643
1103	11	-0.06239255209992223
1103	12	-0.012410256856242086
1103	13	-0.018803543887578655
1103	14	-0.05933515361660824
1103	15	-0.03456147763161965
1103	16	-0.10486706485372821
1103	17	-0.030451021820965707
1103	18	0.021529525739850897
1103	19	-0.004334097043972918
1103	20	0.04150975268468926
1103	21	-0.05425855460417495
1103	22	-0.04874813345995932
1103	23	0.03497718198659783
1103	24	0.06985287023914566
1103	25	-0.0032295493709071366
1103	26	0.031050890804245057
1103	27	-0.05037250396649284
1103	28	0.047590283533992306
1103	29	-0.0969571372323017
1103	30	0.09717148817799881
1103	31	-0.09573211676692495
1103	32	-0.0210166425851952
1103	33	0.023142388768640387
1103	34	0.05109538891510823
1103	35	-0.018524061144802317
1103	36	-0.10832210222840644
1103	37	0.022559817999933784
1103	38	-0.08912980777773782
1103	39	-0.06459880063668777
1103	40	-0.1280721952502546
1103	41	0.06815949406890098
1103	42	0.03967632823110989
1103	43	-0.06514769033311296
1103	44	0.08619947303934339
1103	45	-0.15930326726851263
1103	46	-0.052183483181096335
1103	47	-0.08962480431443254
1103	48	0.11566238114326666
1103	49	0.14772971278823377
1103	50	-0.052173767127627
1103	51	0.010685419731025547
1103	52	0.06601538252665762
1103	53	-0.06258963777983695
1103	54	0.05697551977786475
1103	55	0.002077535563330457
1103	56	0.2728227039870319
1103	57	0.3773614671865886
1103	58	0.11797868076121887
1103	59	0.21816095460067686
1103	60	0.2748890086152471
1103	61	0.11589177486808921
1104	1	-0.059478394671458545
1104	2	-0.0864469356610229
1104	3	-0.024361807978472427
1104	4	0.014567085783077971
1104	5	-0.11853506722412277
1104	6	-0.04683552214481779
1104	7	-0.11405490580526134
1104	8	0.015165088310645558
1104	9	-0.05148788315935349
1104	10	-0.09309307734510952
1104	11	-0.09938588768076073
1104	12	0.022825073898175674
1104	13	-0.11419578085824611
1104	14	-0.08902029361892971
1104	15	-0.09804487943762297
1104	16	-0.060725525466232
1104	17	0.02979587976695086
1104	18	-0.11338544053806743
1104	19	0.03740518493581391
1104	20	0.02427444859556971
1104	21	-0.0025102635639058235
1104	22	0.0987795174989401
1104	23	-0.07385864931350739
1104	24	-0.012988107462952314
1104	25	-0.021202434574902895
1104	26	-0.0020302650220287433
1104	27	-0.006754270999429934
1104	28	-0.08769323521837435
1104	29	-0.05806580402467887
1104	30	-0.00429615062914479
1104	31	-0.0779435078001498
1104	32	0.07441940165633554
1104	33	-0.017296007732873875
1104	34	0.050302572545668056
1104	35	-0.1512086659365579
1104	36	0.03368154710772586
1104	37	0.11937960060159014
1104	38	-0.12380850049259351
1104	39	0.01494615609887262
1104	40	-0.04377891128581268
1104	41	-0.012939192284370904
1104	42	-0.04907101238185163
1104	43	0.17220343637084312
1104	44	0.019243392685382332
1104	45	0.12922998911639638
1104	46	0.03683336738087206
1104	47	0.20155355413189113
1104	48	-0.10922272000391331
1104	49	-0.1288674907841525
1104	50	-0.11250637859332357
1104	51	-0.07539253435513871
1104	52	-0.04716736807473865
1104	53	-0.1262296981957789
1104	54	0.023934838060540642
1104	55	0.08982778459814256
1104	56	0.05314685874478897
1104	57	0.11776243704147049
1104	58	0.13385119779367471
1104	59	0.10993461576477159
1104	60	0.00999834584933268
1104	61	-0.18893921477625497
1201	1	0.0014904345532045227
1201	2	0.03930612212503348
1201	3	0.041123265428019806
1201	4	0.007459584593981106
1201	5	0.07028841986210921
1201	6	-0.02226343464573688
1201	7	-0.06363472595912611
1201	8	-0.06680852815154736
1201	9	0.00418396072605276
1201	10	-0.06664319289105691
1201	11	-0.004890977216021497
1201	12	-0.032341694468861704
1201	13	0.06529525627064459
1201	14	-0.043696190256461875
1201	15	0.025621062399729055
1201	16	0.027386557379237786
1201	17	0.018059064761025923
1201	18	0.01907563687538527
1201	19	-0.009025786052013984
1201	20	0.04087979912545592
1201	21	0.011663155353481613
1201	22	0.00951524572813156
1201	23	0.06379755732172043
1201	24	-0.045578794449760096
1201	25	-0.03924311534755276
1201	26	-0.08003336182178344
1201	27	0.00467884457362544
1201	28	0.06269451887292377
1201	29	0.02286588976780024
1201	30	0.023411983936803438
1201	31	-0.029491767508950772
1201	32	-0.06282797881925006
1201	33	0.07715358143187956
1201	34	0.06963140614360697
1201	35	0.014043072859507788
1201	36	0.02073413943936231
1201	37	-0.06220556092445406
1201	38	0.020742150498826106
1201	39	-0.04705898903674921
1201	40	0.1191735891884278
1201	41	0.013595260780981278
1201	42	0.025740436993324067
1201	43	-0.0024050483371880205
1201	44	-0.009102977919503985
1201	45	0.17049573359584258
1201	46	0.006764787376631887
1201	47	0.47682064610236097
1201	48	0.17493295352200278
1201	49	0.055368265946057704
1201	50	0.12355031321342835
1201	51	0.08358056908862466
1201	52	0.13029262069305503
1201	53	-0.014555678887973045
1201	54	0.00406308332259897
1201	55	0.0756319386457065
1201	56	0.44507471471089133
1201	57	0.5118199268768819
1201	58	0.2903662047507254
1201	59	0.2522187345271334
1201	60	0.5596756301596599
1201	61	0.27797296562681595
1202	1	-0.20003710500906635
1202	2	-0.11231735121425146
1202	3	-0.11233007301191122
1202	4	-0.16902896163463055
1202	5	-0.0984791687679305
1202	6	-0.12670358658559877
1202	7	-0.06787264912699698
1202	8	-0.02243106158034891
1202	9	-0.12024409079140241
1202	10	0.02731640205932706
1202	11	-0.13330069677855505
1202	12	-0.08551324478460742
1202	13	-0.08205236444216979
1202	14	-0.10246834892371481
1202	15	-0.13583539180645943
1202	16	-0.21498823377746135
1202	17	-0.16294771258565532
1202	18	-0.3235134094670187
1202	19	-0.19567295142630314
1202	20	-0.0555498361781801
1202	21	-0.09712870813006447
1202	22	-0.14566064519785016
1202	23	-0.17706560614314526
1202	24	-0.12655448814255266
1202	25	0.029107619281298014
1202	26	-0.07931917786945775
1202	27	-0.09518294417964768
1202	28	-0.05129645576614905
1202	29	-0.15240521335037016
1202	30	-0.13198315776210734
1202	31	-0.05468062845955757
1202	32	0.035025398845299094
1202	33	-0.008770518946121797
1202	34	-0.06402923066424648
1202	35	0.013595320226187368
1202	36	-0.0016544173354873237
1202	37	-0.06979330286352337
1202	38	0.03635243548782796
1202	39	0.08223790981370627
1202	40	-0.1514716001535774
1202	41	-0.03498682504320527
1202	42	-0.10180338078245654
1202	43	0.029346534061325258
1202	44	0.0072501693929680755
1202	45	0.014341077616030873
1202	46	0.022664631403294777
1202	47	-0.16117782561799032
1202	48	0.2580641505942154
1202	49	0.10782655741822111
1202	50	0.15233414675306278
1202	51	-0.06881259472778112
1202	52	0.09459359927308952
1202	53	-0.003410893818490024
1202	54	-0.12205976741850577
1202	55	-0.04332686996224311
1202	56	0.4500138703086307
1202	57	0.2932842630095962
1202	58	0.29721478480131225
1202	59	0.3448933534285885
1202	60	0.46892616255974673
1202	61	0.03959834469012691
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2403	11	0.10858857925705934
2403	12	-0.10234571124591696
2403	13	0.05897677255727274
2403	14	-0.07896349275713987
2403	15	-0.1228137957519877
2403	16	0.006473160960709379
2403	17	0.0398094454207083
2403	18	-0.10330435023356443
2403	19	-0.0013724245701070004
2403	20	-0.07707519428890208
2403	21	-0.05934318741771705
2403	22	0.027983078817030105
2403	23	0.13271237362422994
2403	24	-1.695969644543993E-4
2403	25	-0.1393113071439842
2403	26	0.027526586240355755
2403	27	-0.03991859029387257
2403	28	-0.10959065361495846
2403	29	-0.06750225251952457
2403	30	-0.09726787893229982
2403	31	0.18445485606299172
2403	32	-0.09391129424834332
2403	33	0.008136010367621793
2403	34	-0.15423105193711706
2403	35	0.10429538250630799
2403	36	-0.0941858519665999
2403	37	0.0235460901577369
2403	38	-0.0411442401000528
2403	39	0.05573687461701837
2403	40	0.012449691233243243
2403	41	-0.03619876262841615
2403	42	-0.04994591810844895
2403	43	-0.01837687484978545
2403	44	-0.04989942073648024
2403	45	-0.03346312713409105
2403	46	0.04849910092811882
2403	47	-0.08073883489267786
2403	48	-0.46125447116342855
2403	49	-0.14628982225489295
2403	50	-0.14399132581843332
2403	51	-0.1095740955104958
2403	52	-0.21101666179846745
2403	53	-0.11979056693198942
2403	54	0.03749854866155289
2403	55	-0.16145038660113897
2403	56	-0.20505186047289925
2403	57	-0.7244739891070893
2403	58	-0.7558108510451049
2403	59	-0.5551992251369455
2403	60	-0.6061500043509938
2403	61	-0.4384369698245152
2404	1	-0.06910207372183663
2404	2	0.07405757119418865
2404	3	-0.09011671839632385
2404	4	-0.06532712141348185
2404	5	0.010936494509763861
2404	6	0.008949589752539566
2404	7	-0.12399014105940154
2404	8	0.006794449367284017
2404	9	0.026631427288876353
2404	10	-0.006606048792924923
2404	11	0.054094000392673536
2404	12	0.0033421014183261377
2404	13	0.008350957440384832
2404	14	0.011428347314492271
2404	15	0.00650608044757206
2404	16	0.010398374594331017
2404	17	0.024315898226660693
2404	18	-0.06752777498976315
2404	19	0.02220221580518711
2404	20	-0.2067425292703149
2404	21	1.2676761702579142E-4
2404	22	-0.004597264943306811
2404	23	-0.02228538784580536
2404	24	0.024740375199918674
2404	25	0.04124949327646732
2404	26	0.07194291388328367
2404	27	0.10913538747655593
2404	28	-0.015686268042770735
2404	29	0.043684557672605706
2404	30	-0.06360330804876213
2404	31	-0.06447162282670149
2404	32	-0.03467784477098945
2404	33	0.02427191833483988
2404	34	0.01645393823281438
2404	35	-0.03615388072535097
2404	36	-0.061675583926970035
2404	37	-0.20910822241890514
2404	38	0.1254121221914275
2404	39	-0.03580342865512165
2404	40	0.11024327930480382
2404	41	-0.13103457132169893
2404	42	-0.02234095051846907
2404	43	-0.044686414880537484
2404	44	-0.05191604425466778
2404	45	0.11618746753626397
2404	46	-0.07748341907266365
2404	47	-0.18849680113954292
2404	48	-0.3998457545223886
2404	49	-0.19939456599187366
2404	50	-0.07346692584179237
2404	51	-0.06474120663890659
2404	52	-0.0013442657927811297
2404	53	0.0705574758142049
2404	54	-0.044025792918660045
2404	55	0.048571414734198015
2404	56	-0.33751249154369134
2404	57	-0.3661437378077679
2404	58	-0.23673176280809827
2404	59	-0.18312731966130968
2404	60	0.06786455582653161
2404	61	-0.13436771165387995
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	8.0
14	23.0
15	89.0
16	279.0
17	588.0
18	929.0
19	1149.0
20	1224.0
21	1205.0
22	1299.0
23	1514.0
24	1890.0
25	2071.0
26	2497.0
27	2949.0
28	4256.0
29	5042.0
30	5865.0
31	6647.0
32	7807.0
33	9037.0
34	11509.0
35	16159.0
36	25215.0
37	39957.0
38	95572.0
39	394038.0
40	287753.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	28.777028886574463	23.059829152074084	25.338916739966866	22.82422522138459
2	29.835813985112853	21.91844985435547	18.25915121453186	29.986584945999823
3	31.99949059489235	17.597680048263975	18.202058989543165	32.20077036730051
4	30.040439426660235	18.69808142063587	19.673074162692338	31.588404990011558
5	27.78351578022623	19.60842720093765	20.925973292926283	31.68208372590983
6	27.18507270354889	19.872087514070696	22.57657535148413	30.366264430896283
7	28.137293310496442	21.734545976509086	22.59373539642402	27.534425316570456
8	26.94699057060927	23.37090196002249	23.11986884976974	26.5622386195985
9	27.183022132140984	23.039788640050247	22.79900838683706	26.97818084097171
10	28.749658687785395	22.179412047214946	21.786997434627246	27.283931830372417
11	27.24971966530358	22.95852125741039	22.38878618044381	27.402972896842225
12	28.329939098029183	21.564888173707143	21.940682365409668	28.164490362854007
13	28.48934404379157	22.21696988142301	21.323999995683007	27.969686079102413
14	28.80275769476921	22.215890633313585	21.383142792079614	27.598208879837593
15	28.329507398785413	21.64000384212327	21.947913327742828	28.082575431348488
16	28.55766044911831	22.086704634615156	21.20031816234266	28.155316753923877
17	29.027241301530047	22.25355639233259	21.079874073330593	27.63932823280677
18	28.55323553186966	21.799840487129426	21.641622714287408	28.005301266713506
19	29.278598186215625	22.305576151206978	20.895106796996668	27.52071886558073
20	28.44919601412088	22.36385554911604	21.69018887921163	27.496759557551442
21	28.496790855746617	22.58898670474254	21.124554945060876	27.789667494449965
22	29.20585686364024	22.18513206219491	21.209383846461847	27.399627227703004
23	28.955471302253144	22.14563158138988	21.31061731912611	27.588279797230864
24	29.055409677186102	21.503478956280738	21.24877640245594	28.192334964077226
25	28.564243862585815	22.615104508990676	21.069081592236323	27.75157003618719
26	28.427287277499513	22.73371387621672	21.3426709879761	27.496327858307673
27	28.692458537985754	22.401413383324105	21.13157005777215	27.77455802091799
28	28.688357395169934	22.967586941529575	20.91064796977242	27.43340769352807
29	28.476716840911276	23.179227495788236	21.010802194327255	27.333253468973233
30	28.598132253221824	22.667232192676007	21.00303160793938	27.731603946162785
31	28.818514717166842	22.588770855120654	21.4892328812363	27.1034815464762
32	29.190531540486376	22.748283725693984	21.14311801254302	26.918066721276624
33	28.749490052516496	22.26328183853066	21.711459302413427	27.275768806539414
34	28.7355205375519	22.623414719433267	21.030228660296945	27.610836082717892
35	28.550645336407033	22.989063978907176	21.199778538287944	27.260512146397847
36	29.095881481289616	22.709754568187435	20.98705873591986	27.20730521460309
37	29.09933507523978	22.902939979774892	21.250719049052904	26.747005895932425
38	28.842474025196125	23.158182157654405	20.828840963077845	27.170502854071625
39	29.049689662206134	22.800519334190255	20.592269777491417	27.557521226112193
40	29.40886343302348	23.125480939938765	20.458550936733396	27.007104690304356
41	29.239421479843426	23.67330728028397	20.65972278433061	26.427548455541995
42	29.334287388662066	23.44008176383677	20.640620092793753	26.58501075470741
43	29.452357131833395	23.421950395598394	20.922303849354233	26.20338862321398
44	29.55413022855237	23.474509778527494	21.002491983884667	25.968868009035468
45	29.845635142908638	23.29287232171091	20.726959941547925	26.134532593832528
46	29.9913336376813	23.51379440971064	20.049947602504286	26.444924350103772
47	30.07335649399776	23.764611670341505	20.148159180462155	26.01387265519858
48	30.089221441206345	24.143427756750427	20.107687376358637	25.65966342568459
49	30.60650506005476	23.771303008619952	19.85546709318552	25.766724838139766
50	30.222940281964362	24.21703247781336	20.08545486530444	25.47457237491784
51	30.52685654957904	23.928117758919715	19.960801708665606	25.584223982835635
52	30.683779224689744	24.26365599614061	19.914717814393068	25.13784696477658
53	30.860667989824847	23.850088120608135	19.647495982498913	25.6417479070681
54	30.765694156195263	23.484546785945167	19.519389231909916	26.230369825949655
55	31.32970921818188	23.89887013515424	19.91957443088549	24.85184621577839
56	30.69673020200287	24.496989437398753	20.536040950990262	24.270239409608113
57	31.447023487676606	24.66038760116602	19.787258612669724	24.105330298487647
58	31.00787743195071	25.276530346837966	19.990804806107683	23.724787415103645
59	30.851170606461892	25.800289454342952	19.425602571200695	23.922937367994464
60	30.784473073299296	24.452200640857527	20.86456407549988	23.898762210343296
61	31.1508778064498	25.659555500873648	19.412004045021913	23.777562647654634
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.5
3	1.0
4	0.5
5	0.0
6	1.0
7	1.5
8	1.0
9	3.0
10	5.0
11	5.5
12	7.5
13	9.0
14	15.5
15	22.0
16	30.0
17	45.0
18	52.0
19	64.0
20	116.5
21	157.0
22	219.0
23	281.0
24	373.0
25	524.5
26	584.0
27	825.5
28	1067.0
29	1376.5
30	2040.0
31	2394.0
32	2823.0
33	3252.0
34	3935.5
35	5444.5
36	6270.0
37	7263.0
38	8256.0
39	9763.0
40	13099.0
41	14928.0
42	17237.5
43	22201.0
44	24855.0
45	27688.5
46	30522.0
47	33776.5
48	40744.5
49	44458.0
50	48260.0
51	52062.0
52	55929.5
53	62499.5
54	65202.0
55	66700.0
56	68198.0
57	68709.0
58	68927.0
59	68634.0
60	67159.5
61	62693.5
62	59702.0
63	55897.5
64	52093.0
65	47800.5
66	39010.0
67	34512.0
68	30331.0
69	26150.0
70	22156.5
71	15234.5
72	12306.0
73	10160.5
74	8015.0
75	6424.5
76	3811.0
77	2788.0
78	2178.0
79	1568.0
80	1191.0
81	597.5
82	381.0
83	297.5
84	160.5
85	107.0
86	79.0
87	51.0
88	50.0
89	46.0
90	43.0
91	43.5
92	44.0
93	44.5
94	53.0
95	61.0
96	62.0
97	63.0
98	71.0
99	203.5
100	328.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0017267969750833988
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	5.396240547135622E-4
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	926571.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.62803233078284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.10666605899576	68.61983022569848
2	11.312583993116599	18.24222775883403
3	2.2312855162863374	5.397124822390269
4	0.5945885214945356	1.917620101382951
5	0.22671381147967487	0.9139744260909115
6	0.13229053435089136	0.6399795288420125
7	0.08450242107221032	0.4769284756767718
8	0.05694261558567769	0.3672936840353087
9	0.04336712614239095	0.3146945443831652
>10	0.20718627077045948	2.7352399623725026
>50	0.0027255364223288682	0.15209334210179448
>100	0.0011475942830858393	0.2229931281918325
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.5482580395889791	0.0	0.0	0.0	1.0792481094271243E-4
2	0.5490135132655781	0.0	0.0	0.0	1.0792481094271243E-4
3	0.549445212509349	0.0	0.0	0.0	1.0792481094271243E-4
4	0.5497689869421771	0.0	0.0	0.0	1.0792481094271243E-4
5	0.550200686185948	0.0	0.0	0.0	1.0792481094271243E-4
6	0.552467107215745	0.0	0.0	0.0	1.0792481094271243E-4
7	0.5549493778674274	0.0	0.0	0.0	1.0792481094271243E-4
8	0.5622882650115317	0.0	0.0	0.0	1.0792481094271243E-4
9	0.5632595883100162	0.0	0.0	0.0	1.0792481094271243E-4
10	0.5653101597179278	0.0	0.0	0.0	1.0792481094271243E-4
11	0.5677924303696101	0.0	0.0	0.0	2.1584962188542486E-4
12	0.5731886709167457	0.0	0.0	0.0	2.1584962188542486E-4
13	0.5806354828717929	1.0792481094271243E-4	0.0	0.0	2.1584962188542486E-4
14	0.5925072120754913	1.0792481094271243E-4	0.0	0.0	2.1584962188542486E-4
15	0.6064295126871011	1.0792481094271243E-4	0.0	0.0	2.1584962188542486E-4
16	0.6227261591394507	1.0792481094271243E-4	0.0	0.0	2.1584962188542486E-4
17	0.644742820571764	1.0792481094271243E-4	0.0	0.0	2.1584962188542486E-4
18	0.6726953466059266	1.0792481094271243E-4	0.0	0.0	3.237744328281373E-4
19	0.7061520379981674	1.0792481094271243E-4	0.0	0.0	3.237744328281373E-4
20	0.7520200826488203	1.0792481094271243E-4	0.0	0.0	3.237744328281373E-4
21	0.8269199014430626	1.0792481094271243E-4	0.0	0.0	3.237744328281373E-4
22	0.9030068931576749	1.0792481094271243E-4	0.0	0.0	3.237744328281373E-4
23	0.9961460050012357	1.0792481094271243E-4	0.0	0.0	3.237744328281373E-4
24	1.1074164850831723	1.0792481094271243E-4	0.0	0.0	3.237744328281373E-4
25	1.2481504385524693	1.0792481094271243E-4	0.0	0.0	3.237744328281373E-4
26	1.4140308729714182	1.0792481094271243E-4	0.0	0.0	3.237744328281373E-4
27	1.6096985552105558	1.0792481094271243E-4	0.0	0.0	3.237744328281373E-4
28	1.8615950639508467	1.0792481094271243E-4	0.0	0.0	3.237744328281373E-4
29	2.142955046078498	1.0792481094271243E-4	0.0	0.0	3.237744328281373E-4
30	2.466837403717578	1.0792481094271243E-4	0.0	0.0	3.237744328281373E-4
31	2.86043918922565	1.0792481094271243E-4	0.0	0.0	3.237744328281373E-4
32	3.279511230116203	1.0792481094271243E-4	1.0792481094271243E-4	0.0	3.237744328281373E-4
33	3.7498475562045432	3.237744328281373E-4	1.0792481094271243E-4	0.0	3.237744328281373E-4
34	4.268534197595219	3.237744328281373E-4	1.0792481094271243E-4	0.0	4.316992437708497E-4
35	4.844744763218361	3.237744328281373E-4	1.0792481094271243E-4	0.0	4.316992437708497E-4
36	5.48452304248676	3.237744328281373E-4	1.0792481094271243E-4	0.0	4.316992437708497E-4
37	6.168010870186958	3.237744328281373E-4	1.0792481094271243E-4	0.0	4.316992437708497E-4
38	6.897042968104981	3.237744328281373E-4	1.0792481094271243E-4	0.0	4.316992437708497E-4
39	7.692988448807485	3.237744328281373E-4	1.0792481094271243E-4	0.0	4.316992437708497E-4
40	8.540090289896835	3.237744328281373E-4	1.0792481094271243E-4	0.0	4.316992437708497E-4
41	9.435866220721348	3.237744328281373E-4	1.0792481094271243E-4	0.0	4.316992437708497E-4
42	10.394022692270749	3.237744328281373E-4	1.0792481094271243E-4	0.0	4.316992437708497E-4
43	11.388333975485958	3.237744328281373E-4	1.0792481094271243E-4	0.0	5.396240547135622E-4
44	12.381350160969856	3.237744328281373E-4	1.0792481094271243E-4	0.0	5.396240547135622E-4
45	13.440524255561636	3.237744328281373E-4	1.0792481094271243E-4	0.0	5.396240547135622E-4
46	14.56315813898773	3.237744328281373E-4	1.0792481094271243E-4	0.0	5.396240547135622E-4
47	15.672193496235042	3.237744328281373E-4	2.1584962188542486E-4	0.0	5.396240547135622E-4
48	16.870374747321037	3.237744328281373E-4	2.1584962188542486E-4	0.0	5.396240547135622E-4
49	18.084636795237493	3.237744328281373E-4	2.1584962188542486E-4	0.0	5.396240547135622E-4
50	19.355991068142647	3.237744328281373E-4	2.1584962188542486E-4	0.0	5.396240547135622E-4
>>END_MODULE