##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-35A_S44_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	926571
Sequences flagged as poor quality	0
Sequence length	61
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.49274907157682	40.0	40.0	40.0	40.0	40.0
2	39.225969731407524	40.0	40.0	40.0	40.0	40.0
3	39.168523513038934	40.0	40.0	40.0	40.0	40.0
4	39.0553643487655	40.0	40.0	40.0	40.0	40.0
5	39.06487468310577	40.0	40.0	40.0	40.0	40.0
6	39.18887597388651	40.0	40.0	40.0	40.0	40.0
7	39.239531563150585	40.0	40.0	40.0	40.0	40.0
8	39.26234255119144	40.0	40.0	40.0	40.0	40.0
9	39.25730462101663	40.0	40.0	40.0	40.0	40.0
10	39.26944400375147	40.0	40.0	40.0	40.0	40.0
11	39.26121581616519	40.0	40.0	40.0	40.0	40.0
12	39.27267743108731	40.0	40.0	40.0	40.0	40.0
13	39.100101341397476	40.0	40.0	40.0	40.0	40.0
14	39.272910548678944	40.0	40.0	40.0	40.0	40.0
15	39.18173998538698	40.0	40.0	40.0	40.0	40.0
16	39.361555671394854	40.0	40.0	40.0	40.0	40.0
17	39.303595730926176	40.0	40.0	40.0	40.0	40.0
18	39.391658059662994	40.0	40.0	40.0	40.0	40.0
19	39.34521153802569	40.0	40.0	40.0	40.0	40.0
20	39.35832656105145	40.0	40.0	40.0	40.0	40.0
21	39.34096361746698	40.0	40.0	40.0	40.0	40.0
22	39.394334594974374	40.0	40.0	40.0	40.0	40.0
23	39.38812244285651	40.0	40.0	40.0	40.0	40.0
24	39.39638084938985	40.0	40.0	40.0	40.0	40.0
25	39.3479830471707	40.0	40.0	40.0	40.0	40.0
26	39.31737557078734	40.0	40.0	40.0	40.0	40.0
27	39.32865587202708	40.0	40.0	40.0	40.0	40.0
28	39.334954363993695	40.0	40.0	40.0	40.0	40.0
29	39.34937311873564	40.0	40.0	40.0	40.0	40.0
30	39.34541011967782	40.0	40.0	40.0	40.0	40.0
31	39.29783254602184	40.0	40.0	40.0	40.0	40.0
32	39.32141627570904	40.0	40.0	40.0	40.0	40.0
33	39.32546561461561	40.0	40.0	40.0	40.0	40.0
34	39.31903329588342	40.0	40.0	40.0	40.0	40.0
35	39.320738507896316	40.0	40.0	40.0	40.0	40.0
36	39.28984611001208	40.0	40.0	40.0	40.0	40.0
37	39.296852588738474	40.0	40.0	40.0	40.0	40.0
38	39.27534964940625	40.0	40.0	40.0	40.0	40.0
39	38.03942709193359	40.0	40.0	40.0	40.0	40.0
40	39.284121778039676	40.0	40.0	40.0	40.0	40.0
41	39.28961299242044	40.0	40.0	40.0	40.0	40.0
42	39.24712515284851	40.0	40.0	40.0	40.0	40.0
43	39.27010450359444	40.0	40.0	40.0	40.0	40.0
44	39.22091237476675	40.0	40.0	40.0	40.0	40.0
45	39.07762276177433	40.0	40.0	40.0	40.0	40.0
46	39.07628449411864	40.0	40.0	40.0	40.0	40.0
47	39.15363204762506	40.0	40.0	40.0	40.0	40.0
48	39.24309739890413	40.0	40.0	40.0	40.0	40.0
49	39.23445478004384	40.0	40.0	40.0	40.0	40.0
50	39.19964255302616	40.0	40.0	40.0	40.0	40.0
51	39.17627898995328	40.0	40.0	40.0	40.0	40.0
52	39.17674090814411	40.0	40.0	40.0	40.0	40.0
53	39.11051392715723	40.0	40.0	40.0	40.0	40.0
54	39.018203677861706	40.0	40.0	40.0	40.0	40.0
55	39.07114295612533	40.0	40.0	40.0	40.0	40.0
56	39.072705707387776	40.0	40.0	40.0	40.0	40.0
57	39.02239547751872	40.0	40.0	40.0	40.0	40.0
58	38.98974606371233	40.0	40.0	40.0	40.0	40.0
59	38.96095604114525	40.0	40.0	40.0	40.0	40.0
60	38.924166631591106	40.0	40.0	40.0	40.0	40.0
61	38.80358439882103	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.031519817310460496
1101	2	-0.10535539809124117
1101	3	0.13680003520162387
1101	4	-0.0022901516759574747
1101	5	-0.11322343290223102
1101	6	0.01000399942719099
1101	7	-0.1441023784312847
1101	8	-0.0314782185641036
1101	9	-0.15466880785199066
1101	10	0.05217217481496306
1101	11	-0.05106048517825457
1101	12	-0.022496755574735516
1101	13	-0.01072734884670723
1101	14	7.676219000458673E-4
1101	15	0.07883304910107114
1101	16	0.02838917776894334
1101	17	0.10342389879100722
1101	18	-0.04283983795723856
1101	19	-0.009543581156101766
1101	20	0.005920139674287839
1101	21	-0.0038068453450321726
1101	22	-0.12334478146874517
1101	23	-0.07162745152267291
1101	24	-0.019129225114582482
1101	25	0.020928008722819413
1101	26	0.056444386644265876
1101	27	-0.03315787591971997
1101	28	0.01812350955745501
1101	29	0.10197188180506345
1101	30	-0.0056409718515979534
1101	31	0.05527230737304478
1101	32	-0.02957891403072921
1101	33	-0.04713301593005781
1101	34	0.05227733879924301
1101	35	-0.10028059342450035
1101	36	-0.05264173132678707
1101	37	-0.07267745455681762
1101	38	0.013999727907439308
1101	39	1.1741469463357035
1101	40	0.022514337208988877
1101	41	-0.0678475603823756
1101	42	0.06272202461420306
1101	43	-0.03597466879843125
1101	44	-0.011415861865110344
1101	45	-0.017744723246146066
1101	46	-0.05724841951717963
1101	47	-0.011123421202889006
1101	48	-0.039023874517354784
1101	49	0.01099887413244005
1101	50	-0.1630510034707342
1101	51	-0.04875696858554335
1101	52	-0.014314968813046391
1101	53	-0.1666205141312176
1101	54	-0.007130895576011653
1101	55	-0.06859946475507428
1101	56	-0.01852702521756555
1101	57	0.046387565895486205
1101	58	0.008077928598027029
1101	59	-0.10486507754971086
1101	60	0.06740393022077029
1101	61	0.0564785638350358
1102	1	0.05524372734002014
1102	2	0.014623421541926973
1102	3	0.07784635617552027
1102	4	-0.05193423650233342
1102	5	-0.0515742763637661
1102	6	-0.1228693407130379
1102	7	0.08294039198239744
1102	8	-0.09654996683065065
1102	9	-0.08726468699548207
1102	10	-0.09236257608963427
1102	11	-0.07839645264260753
1102	12	-0.14189496908043253
1102	13	-0.1511449511604468
1102	14	0.0033848713282580434
1102	15	0.007467783636549541
1102	16	-0.08028681416768535
1102	17	0.0319080555691329
1102	18	-0.04895609845585369
1102	19	-0.12506339999251992
1102	20	0.042907101358807154
1102	21	-0.02514999894067671
1102	22	-0.033557480464260436
1102	23	-0.0026849957396279933
1102	24	-0.126591551283191
1102	25	-0.07035647833156133
1102	26	0.01713409607893368
1102	27	-0.059868470896297765
1102	28	0.02996172483571513
1102	29	-0.015362557272439403
1102	30	-0.044563287294366205
1102	31	-0.039227670469863085
1102	32	-0.023708642421013337
1102	33	0.004730847307357067
1102	34	-0.0718882659852369
1102	35	0.004858856048748805
1102	36	-0.12707236200792948
1102	37	-0.0020081025114464524
1102	38	-0.03888562227854919
1102	39	1.1985975183955233
1102	40	-0.0015062064807196407
1102	41	0.048464939561100095
1102	42	-0.005740662398395102
1102	43	-0.19681206200353074
1102	44	0.01114547935313226
1102	45	0.08259070792625067
1102	46	0.017600482712182952
1102	47	-0.05666392300213374
1102	48	0.09449247334349309
1102	49	-0.05805165397495671
1102	50	0.09833706118455154
1102	51	-0.15182676521965988
1102	52	0.06277315580202725
1102	53	0.05967208060842211
1102	54	0.09757129055980585
1102	55	-0.12796508473299184
1102	56	-0.1595451298189161
1102	57	-0.0901263193633568
1102	58	-0.03798756051741492
1102	59	-0.10058242908139903
1102	60	0.04569902825910077
1102	61	-0.008867208818777783
1103	1	0.08181623278493788
1103	2	-0.01739283775728495
1103	3	0.0028042966270263037
1103	4	0.055673930595645515
1103	5	0.09701051065562183
1103	6	0.020360422728785466
1103	7	0.006667287177272385
1103	8	0.046854462510239614
1103	9	-0.12146520158450613
1103	10	0.08383572513312032
1103	11	0.05138403657095836
1103	12	0.05061060625506997
1103	13	-0.046387797310998735
1103	14	0.1032411329377183
1103	15	-0.01850573421849333
1103	16	-0.037800615102597135
1103	17	-0.06896452868503644
1103	18	0.030524803807630008
1103	19	-0.08950328604514368
1103	20	0.020052417797522537
1103	21	0.0816137167572748
1103	22	0.10332608864474935
1103	23	0.11373005887723764
1103	24	0.04976744256670429
1103	25	0.06503521111153532
1103	26	0.006448735037530184
1103	27	-0.013300213326047583
1103	28	0.0425469850832485
1103	29	-0.01920334394902312
1103	30	-0.018421624400481562
1103	31	0.008697391804354027
1103	32	-0.0617932608295888
1103	33	0.04183873400833704
1103	34	-0.08546888896959359
1103	35	-0.09872966649200521
1103	36	0.010862928701335761
1103	37	0.09104317567031472
1103	38	-0.05521944560794623
1103	39	1.2287374001630127
1103	40	0.04506437996818846
1103	41	0.08562173271382534
1103	42	0.02576068183036284
1103	43	0.058432572725820364
1103	44	0.07813567384244635
1103	45	0.01832157525655731
1103	46	0.03519842137511375
1103	47	0.06477778330204842
1103	48	-0.007697324677522488
1103	49	-0.07038492955209819
1103	50	-0.0586279627842643
1103	51	-0.011455001662326936
1103	52	0.15026446908464663
1103	53	0.16343340594531242
1103	54	-0.0036413099778869196
1103	55	0.058768597745832096
1103	56	0.17741519913053594
1103	57	0.11298683246648977
1103	58	0.11459544537557775
1103	59	0.04636841667417002
1103	60	0.18055274673307053
1103	61	0.1775776927069188
1104	1	-0.031234143671888148
1104	2	-0.06412114887271514
1104	3	-0.17996344674617148
1104	4	-0.16591753041493718
1104	5	-0.059688573391483146
1104	6	-0.02359350725957654
1104	7	0.03602720581002927
1104	8	-0.04936677944493795
1104	9	0.012776942152861182
1104	10	-0.042299692664443
1104	11	-0.21336853805853906
1104	12	-0.09156351050464195
1104	13	0.01076878701704942
1104	14	0.01790314079636346
1104	15	0.025646280820730283
1104	16	-0.09356155692082524
1104	17	-0.02349210536204538
1104	18	0.04097664723663286
1104	19	-0.20511353085160522
1104	20	0.06752997609451938
1104	21	-0.0193872793819736
1104	22	0.020460350311296338
1104	23	0.0767329139684989
1104	24	-0.06327579140030792
1104	25	-0.02495254189926044
1104	26	-0.0212312699907784
1104	27	-0.025952895949984622
1104	28	0.03403123256003937
1104	29	0.07751072980337881
1104	30	0.06688589311766435
1104	31	-0.09048084093415554
1104	32	0.008026957940110435
1104	33	0.12363786052945613
1104	34	-0.06497994268683271
1104	35	0.006834428306930818
1104	36	-0.05206573972905204
1104	37	-0.09450966256467552
1104	38	-0.08244280745410038
1104	39	1.1846534768600208
1104	40	-0.08746261388165522
1104	41	-0.05785184102619212
1104	42	-0.0030149811355428824
1104	43	-0.0014267297481467267
1104	44	-0.019985470403469208
1104	45	-0.1382194216826491
1104	46	0.054805984414727504
1104	47	-0.04012825010195087
1104	48	0.009058480262034152
1104	49	0.07278437412180949
1104	50	0.06696795894928442
1104	51	-0.04722268599093837
1104	52	0.08334981517161566
1104	53	-0.0064107163569673276
1104	54	-0.013856498911032133
1104	55	0.060560410357368255
1104	56	0.08274625829511706
1104	57	0.07172657347028633
1104	58	0.016723812107194647
1104	59	-0.11916640343984142
1104	60	-0.05534607370854161
1104	61	0.10756938837209162
1201	1	0.01449177740176566
1201	2	0.06629392882294383
1201	3	0.02622977898100487
1201	4	-0.08336267996120483
1201	5	-0.0011633565267246126
1201	6	0.028322742715893412
1201	7	0.10637941305078158
1201	8	0.02340334251141485
1201	9	-0.018156480390231877
1201	10	0.024839841806375773
1201	11	0.0961437019630651
1201	12	0.0257677283928075
1201	13	-0.06208169848154199
1201	14	0.09753126317695404
1201	15	0.04007994679060545
1201	16	0.1143258444119013
1201	17	0.0527593465548577
1201	18	0.10836801862178902
1201	19	0.050397679395310035
1201	20	0.007306845785038263
1201	21	0.11246312855644902
1201	22	-0.027964290645890344
1201	23	0.04423006620227454
1201	24	0.039940899546742514
1201	25	-0.05680220226985
1201	26	0.06992388117210879
1201	27	0.09737383698234225
1201	28	-8.245849661250304E-4
1201	29	0.037287375223542085
1201	30	0.10666444483580761
1201	31	0.06046712180332747
1201	32	0.06872432659478278
1201	33	0.03896365526414769
1201	34	0.02006664480617104
1201	35	0.002758781917727049
1201	36	-0.05532980778762919
1201	37	0.027538729370192527
1201	38	0.007702659901944742
1201	39	1.3816586266679423
1201	40	0.06858142533949518
1201	41	-0.014306481880588251
1201	42	0.05672363177102824
1201	43	0.036219384721313475
1201	44	0.03369831027134751
1201	45	0.048621608951748385
1201	46	0.015464759879925793
1201	47	0.12465999663655225
1201	48	0.0210205000781869
1201	49	0.15573543552796565
1201	50	0.027510861400088515
1201	51	0.02877412563224624
1201	52	0.05574213503346215
1201	53	0.069962215333085
1201	54	0.09233504478851273
1201	55	0.05886016076316025
1201	56	0.03847015159102085
1201	57	0.18397014602221162
1201	58	-0.014724497782097501
1201	59	0.08655725624111454
1201	60	0.12898079285152875
1201	61	0.15671963735890415
1202	1	0.09686901084555899
1202	2	0.1380860679889011
1202	3	0.002600651377051122
1202	4	-0.05278413545330096
1202	5	-0.10630381578771164
1202	6	0.15857897933650378
1202	7	-0.036898654286034116
1202	8	0.037065348480211924
1202	9	0.11805838166679905
1202	10	0.07166792144713696
1202	11	0.03477846347928448
1202	12	-0.051627794450901376
1202	13	-0.06449808252748568
1202	14	0.009821702918145547
1202	15	-0.04648432883999476
1202	16	0.05732434388551866
1202	17	-0.10732054583649386
1202	18	0.038359489119144996
1202	19	0.11263159499208797
1202	20	-0.011071364573588482
1202	21	0.024618502420644006
1202	22	-0.01369356312186909
1202	23	-0.04476817054045057
1202	24	-0.05252186945175907
1202	25	-0.01273464712874528
1202	26	0.09201085249559071
1202	27	0.022416900794489436
1202	28	0.030633737959732343
1202	29	0.07065512260224693
1202	30	0.09891480886609827
1202	31	-0.01773972141109681
1202	32	-0.04185299676958465
1202	33	0.060135694037306564
1202	34	-0.047853914255547636
1202	35	-0.04177687824912368
1202	36	-0.299908207978568
1202	37	-0.15964129864210008
1202	38	-0.1695888807186492
1202	39	1.1826372067000221
1202	40	-0.17041209334863083
1202	41	-0.008965829086847066
1202	42	-0.04657863641059379
1202	43	-9.894141729986927E-4
1202	44	-0.16517231697088874
1202	45	-0.09313742982325834
1202	46	-0.03599256392848815
1202	47	-0.07837917061755917
1202	48	-0.05957312370228607
1202	49	-0.15079934763579672
1202	50	-0.037327235318180385
1202	51	-0.028617770785182017
1202	52	-0.07897220063669863
1202	53	-0.0663830870572113
1202	54	0.005627377302204195
1202	55	-0.19032065544178778
1202	56	-0.09248546724197837
1202	57	0.021332327946694818
1202	58	-0.04649643257303637
1202	59	-0.14340350064586005
1202	60	-0.024707523210018678
1202	61	-0.05118097635823915
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2403	9	-0.19045379662268402
2403	10	-0.018338643214782735
2403	11	0.03504104995607804
2403	12	-0.028560582412325175
2403	13	0.061253907911677175
2403	14	0.07668699268905499
2403	15	-0.07860740783475961
2403	16	-0.061250282854537375
2403	17	-0.14728082121564512
2403	18	-0.02293762074636163
2403	19	-0.004118392383560376
2403	20	-0.13575954313239436
2403	21	0.03520275733151834
2403	22	-0.015572066378950922
2403	23	0.042080623413326634
2403	24	-0.0295960501314525
2403	25	0.043573319677634004
2403	26	-0.025170046021472103
2403	27	0.020282544047198314
2403	28	-0.16923496880186661
2403	29	-0.06724317563539017
2403	30	-0.024898081436717234
2403	31	-0.04700128110743407
2403	32	-0.011251572742921212
2403	33	-0.1674594514793668
2403	34	-0.004789732060928031
2403	35	0.019607760441985533
2403	36	-0.06852912400139388
2403	37	-0.031512563938605354
2403	38	0.02097483858313609
2403	39	1.06366533375148
2403	40	-0.0286423278248904
2403	41	-0.08825347910620707
2403	42	0.001427557937063284
2403	43	-0.0262583128247158
2403	44	-0.06012426027913875
2403	45	0.026608736582502956
2403	46	-0.07590570840154953
2403	47	-0.011951524974641359
2403	48	-0.049224523321875324
2403	49	-0.042654117115169754
2403	50	-0.0038508260199208166
2403	51	-0.03994265422034715
2403	52	-0.15471829971259154
2403	53	0.02595982394954177
2403	54	-0.1403437718502829
2403	55	-0.25133497987074094
2403	56	0.0537684673470622
2403	57	0.016289320994886225
2403	58	-0.14042159542714927
2403	59	-0.07137401016461808
2403	60	-0.06470819650868265
2403	61	-0.11783081055844491
2404	1	-0.04053166232144889
2404	2	-0.07813557976663077
2404	3	0.09063285235557572
2404	4	-0.1329193471770438
2404	5	0.05449288083604387
2404	6	-0.08928026475786055
2404	7	-0.06437859614538155
2404	8	-0.03815026930280396
2404	9	-0.07787909785095337
2404	10	0.11278373624524818
2404	11	-0.012487807615016777
2404	12	-0.12232189672139526
2404	13	-0.011738714672169692
2404	14	0.04878712310775768
2404	15	-0.0036692107273310626
2404	16	-0.04316588054204118
2404	17	-0.09878258284140173
2404	18	-0.015877491884893402
2404	19	-0.09627283148581256
2404	20	-0.007468417443803332
2404	21	0.02552646823023963
2404	22	0.09066467276417711
2404	23	-0.08483818607803784
2404	24	-0.057446282743910615
2404	25	-0.0750035592276248
2404	26	-0.03780898087051554
2404	27	-0.11794960124313292
2404	28	-0.02572086606307522
2404	29	0.026260221090510072
2404	30	-0.008149010913285792
2404	31	-0.03040112149052021
2404	32	0.08227518255655752
2404	33	-0.02397162713584322
2404	34	0.0608128218097832
2404	35	0.01825296317493752
2404	36	-0.032425528727614505
2404	37	0.07727512609312015
2404	38	-0.009832200133701008
2404	39	1.321587783287228
2404	40	-0.07910755274848214
2404	41	-0.03820676195906714
2404	42	0.00824534159773549
2404	43	0.0028795611557796974
2404	44	-0.007418558840640799
2404	45	-0.0031929101969367935
2404	46	-0.017919996062381927
2404	47	-0.07516177160224657
2404	48	-0.022938341864687573
2404	49	-0.023394973206890768
2404	50	0.033535544252401905
2404	51	-0.1144253591431692
2404	52	-0.07158124328217497
2404	53	-0.09737344449886365
2404	54	-0.04908961110436394
2404	55	0.043695483602284924
2404	56	0.010061656376315398
2404	57	-0.006651718072326673
2404	58	-0.18333324162261988
2404	59	-0.15611502198016325
2404	60	-0.0405602978070263
2404	61	-0.018655173550676807
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	5.0
17	17.0
18	71.0
19	104.0
20	142.0
21	181.0
22	280.0
23	417.0
24	588.0
25	856.0
26	1055.0
27	1535.0
28	2060.0
29	2428.0
30	3244.0
31	3897.0
32	5276.0
33	6439.0
34	8382.0
35	10540.0
36	15560.0
37	22731.0
38	58768.0
39	413146.0
40	368849.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	31.18821979103598	14.062063241780717	22.815197108478465	31.934519858704835
2	30.972713748064784	17.014015593994735	23.66014073893338	28.3531299190071
3	27.979921603163504	19.249963305445473	19.673893335405495	33.096221755985525
4	28.258816647617934	20.083188444274644	19.640480869787634	32.01751403831978
5	28.4126095032113	20.484344966548704	19.32188682788475	31.781158702355246
6	29.380587132556492	22.72033119965982	21.820885825263257	26.078195842520437
7	28.282883880458165	22.410479067443294	21.23809184617261	28.068545205925936
8	26.964042690738214	23.517140078849867	21.43321990435703	28.085597326054884
9	26.402833673836113	23.755006362167606	22.48116981861077	27.36099014538551
10	27.321921363824252	21.906470200340827	22.10310920587845	28.668499229956474
11	27.124958583853804	22.4067016990603	21.8631923511528	28.605147365933103
12	27.881187734129387	22.281185143933925	21.822828471860227	28.014798650076468
13	27.225652432463356	21.199778538287944	22.032526379521915	29.542042649726792
14	27.55342008329637	21.74782072825504	21.766491720548128	28.932267467900463
15	27.586660925066724	21.63601062411839	21.808150697572014	28.96917775324287
16	27.896836831716083	21.834052652198267	21.33727474742896	28.931835768656693
17	27.88215905742787	21.573630083393503	21.354218942746968	29.189991916431662
18	28.263673264110363	21.997882515209305	21.581292744970433	28.157151475709902
19	27.70624161559125	21.65684011263033	21.960108831379355	28.676809440399065
20	27.63544293961283	21.775125705423545	21.632772879790107	28.956658475173512
21	27.54543364728661	21.93096913242482	22.260247730611038	28.26334948967753
22	28.26205439194622	21.176790553557147	21.592193150875648	28.968961903620986
23	27.739590382172548	21.874956155545554	21.646911030023603	28.738542432258296
24	27.63727766139886	22.43508592433823	21.633744203088593	28.29389221117432
25	27.85226388479674	21.286981785529658	21.555714564777013	29.30503976489659
26	27.955763778490798	21.63924836844667	21.989032680712	28.415955172350525
27	27.880648110074674	22.125557566554534	21.53175525674773	28.462039066623063
28	27.823124185842207	21.653818217923938	21.42955046078498	29.093507135448878
29	27.959972846117566	22.51214423935133	21.38055259661699	28.147330317914115
30	28.47455834469242	22.353710616887426	21.61140376722345	27.560327271196705
31	27.791178441803165	21.889202230589994	22.20628532513968	28.113334002467163
32	27.90309647075076	22.106562799828616	21.525387692902108	28.46495303651852
33	27.850968787065426	23.03784599345328	21.536288098807326	27.57489712067397
34	28.083007130592257	21.96744771852346	21.560571181269435	28.38897396961485
35	28.21683389616122	22.99359682096677	21.208844222407134	27.58072506046488
36	28.67313999682701	22.98248056543967	21.18132339561674	27.16305604211658
37	28.12034911517844	22.543226584902833	21.187583034651418	28.148841265267315
38	28.179276061953157	22.92635966374946	21.067786494505008	27.826577779792373
39	28.50137605990039	23.164492083477253	21.54675818114965	26.787373675472708
40	28.306519414054616	23.464580695920766	20.90697852620037	27.321921363824252
41	28.396528706380842	23.122351120421424	21.41843420525788	27.062685967939853
42	28.40850836039548	23.590744799912798	20.83035191043104	27.170394929260684
43	28.454592254668015	23.996326239435508	20.633389130460593	26.915692375435878
44	28.562732915232615	23.59797576224596	21.01252899130234	26.826762331219083
45	28.4237257587384	24.134362072631237	20.34997857692503	27.09193359170533
46	28.501863321860927	24.002046254415475	20.71120291915029	26.784887504573312
47	29.32640887746325	24.04737467501141	20.01185014424151	26.614366303283827
48	29.22226143490353	24.690390698608095	19.99879124211744	26.088556624370934
49	29.0753757672105	24.391115197863954	19.95184394935736	26.581665085568183
50	29.16527713472578	24.720825495293937	19.690234207632226	26.423663162348056
51	29.016988444490494	25.05107541677864	19.830644386668695	26.101291752062174
52	28.97155209908361	24.89113084696154	19.61630571213647	26.521011341818383
53	29.014290324216923	25.02679233431653	19.47157854066229	26.487338800804256
54	28.986553647804648	25.4537428864059	19.31994418128778	26.239759284501673
55	28.795850506868874	25.321534993001077	19.269003670522817	26.613610829607232
56	28.951370159437325	25.60300289993967	19.023150951195323	26.422475989427685
57	28.761962116232866	25.776761845557438	19.19831291935534	26.262963118854355
58	28.507799186462773	25.769099183980504	18.99325577856419	26.72984585099253
59	28.481033833348985	26.05143048940664	18.89299362919841	26.57454204804597
60	28.553667231113426	26.182667059512976	18.875401885014746	26.388263824358845
61	28.582699005257016	26.038371587282573	18.838383674861397	26.540545732599014
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	1.0
4	1.0
5	1.0
6	2.5
7	3.5
8	3.0
9	2.0
10	1.0
11	2.5
12	8.5
13	13.0
14	16.5
15	20.0
16	23.0
17	42.5
18	59.0
19	66.5
20	119.5
21	165.0
22	190.5
23	216.0
24	317.0
25	529.5
26	641.0
27	864.0
28	1087.0
29	1385.5
30	1967.5
31	2251.0
32	2829.5
33	3408.0
34	3911.5
35	5181.0
36	5947.0
37	7191.5
38	8436.0
39	9891.5
40	13146.5
41	14946.0
42	17407.5
43	22277.5
44	24686.0
45	27638.0
46	30590.0
47	33918.5
48	40530.0
49	43813.0
50	47679.0
51	51545.0
52	55118.0
53	61864.5
54	65038.0
55	67508.5
56	69979.0
57	70095.0
58	70135.0
59	70059.0
60	67902.5
61	64650.0
62	63554.0
63	57290.0
64	51026.0
65	46534.0
66	37722.5
67	33403.0
68	29297.0
69	25191.0
70	21408.0
71	14886.5
72	12148.0
73	10093.5
74	8039.0
75	6466.5
76	3860.0
77	2826.0
78	2184.5
79	1543.0
80	1170.0
81	610.0
82	423.0
83	314.5
84	153.0
85	100.0
86	73.0
87	46.0
88	38.0
89	22.5
90	15.0
91	12.0
92	9.0
93	5.5
94	3.5
95	5.0
96	3.0
97	1.0
98	2.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03421216506883984
3	3.237744328281373E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.1385614270250204
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	926571.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.83361427133599
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.38821262999639	65.73794189249283
2	12.616542952040557	19.89215361037816
3	2.514361137684295	5.946485282011237
4	0.6556834763740519	2.0675959304224256
5	0.2623405486118235	1.0340626808497577
6	0.1402440870413847	0.6633568956993715
7	0.09669397411992976	0.5335914820693161
8	0.06028635193206054	0.3802072811230457
9	0.0428924657785767	0.30432312921013144
>10	0.2203721025107879	2.766778182288178
>50	0.0016295633132430748	0.08537041027502003
>100	5.925684775429363E-4	0.07147290162559816
>500	0.0	0.0
>1k	1.4814211938573408E-4	0.5166603215549777
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTATCATCTGGGGGGGGGGGGGGG	4424	0.47745936361055974	TruSeq Adapter, Index 13 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.5473946411014374	0.0	0.0	0.0	0.0
2	0.5481501147780364	0.0	0.0	0.0	1.0792481094271243E-4
3	0.5485818140218073	0.0	0.0	0.0	1.0792481094271243E-4
4	0.5493372876984063	0.0	0.0	0.0	2.1584962188542486E-4
5	0.5500927613750053	0.0	0.0	0.0	2.1584962188542486E-4
6	0.5525750320266877	0.0	0.0	0.0	2.1584962188542486E-4
7	0.5545176786236564	0.0	0.0	0.0	2.1584962188542486E-4
8	0.5773977385435115	0.0	0.0	0.0	2.1584962188542486E-4
9	0.5792324603295376	0.0	0.0	0.0	3.237744328281373E-4
10	0.5813909565483919	0.0	0.0	0.0	3.237744328281373E-4
11	0.5833336031453606	0.0	1.0792481094271243E-4	0.0	3.237744328281373E-4
12	0.588837768503439	1.0792481094271243E-4	1.0792481094271243E-4	0.0	3.237744328281373E-4
13	0.5966083548913144	1.0792481094271243E-4	1.0792481094271243E-4	0.0	3.237744328281373E-4
14	0.6082642344731273	1.0792481094271243E-4	1.0792481094271243E-4	0.0	3.237744328281373E-4
15	0.621862760651909	1.0792481094271243E-4	1.0792481094271243E-4	0.0	4.316992437708497E-4
16	0.6380514822933159	1.0792481094271243E-4	1.0792481094271243E-4	0.0	4.316992437708497E-4
17	0.659420594859973	1.0792481094271243E-4	1.0792481094271243E-4	0.0	6.475488656562746E-4
18	0.6826244292126561	1.0792481094271243E-4	1.0792481094271243E-4	0.0	6.475488656562746E-4
19	0.7166207446596106	1.0792481094271243E-4	1.0792481094271243E-4	0.0	6.475488656562746E-4
20	0.7618412404446071	1.0792481094271243E-4	1.0792481094271243E-4	0.0	6.475488656562746E-4
21	0.8367410592388494	1.0792481094271243E-4	1.0792481094271243E-4	0.0	7.55473676598987E-4
22	0.9127201261425191	1.0792481094271243E-4	1.0792481094271243E-4	0.0	7.55473676598987E-4
23	1.0060750876079654	1.0792481094271243E-4	1.0792481094271243E-4	0.0	7.55473676598987E-4
24	1.116158394769532	1.0792481094271243E-4	4.316992437708497E-4	0.0	7.55473676598987E-4
25	1.2577557467263707	1.0792481094271243E-4	4.316992437708497E-4	0.0	7.55473676598987E-4
26	1.4243916548219187	1.0792481094271243E-4	4.316992437708497E-4	0.0	8.633984875416994E-4
27	1.6222178332799106	1.0792481094271243E-4	4.316992437708497E-4	0.0	8.633984875416994E-4
28	1.8659120563885552	1.0792481094271243E-4	4.316992437708497E-4	0.0	8.633984875416994E-4
29	2.1372350310985344	1.0792481094271243E-4	4.316992437708497E-4	0.0	8.633984875416994E-4
30	2.4526992534840826	1.0792481094271243E-4	4.316992437708497E-4	0.0	8.633984875416994E-4
31	2.831299490271118	1.0792481094271243E-4	4.316992437708497E-4	0.0	8.633984875416994E-4
32	3.236557155361003	1.0792481094271243E-4	4.316992437708497E-4	0.0	9.713232984844118E-4
33	3.6935108048924477	1.0792481094271243E-4	4.316992437708497E-4	0.0	9.713232984844118E-4
34	4.197627596805857	1.0792481094271243E-4	4.316992437708497E-4	0.0	9.713232984844118E-4
35	4.757649440787592	1.0792481094271243E-4	4.316992437708497E-4	0.0	9.713232984844118E-4
36	5.378864652573845	1.0792481094271243E-4	4.316992437708497E-4	0.0	9.713232984844118E-4
37	6.042818089493411	1.0792481094271243E-4	4.316992437708497E-4	0.0	9.713232984844118E-4
38	6.7594388341530225	1.0792481094271243E-4	4.316992437708497E-4	0.0	9.713232984844118E-4
39	7.538332194726578	1.0792481094271243E-4	4.316992437708497E-4	0.0	0.0010792481094271244
40	8.389966877875521	1.0792481094271243E-4	4.316992437708497E-4	0.0	0.0011871729203698368
41	9.293729244709796	1.0792481094271243E-4	4.316992437708497E-4	0.0	0.0011871729203698368
42	10.255878934264077	1.0792481094271243E-4	4.316992437708497E-4	0.0	0.0011871729203698368
43	11.261954021872041	1.0792481094271243E-4	4.316992437708497E-4	0.0	0.0011871729203698368
44	12.267597410236236	1.0792481094271243E-4	4.316992437708497E-4	0.0	0.0011871729203698368
45	13.34447117382262	1.0792481094271243E-4	4.316992437708497E-4	0.0	0.0012950977313125492
46	14.490524741223284	1.0792481094271243E-4	4.316992437708497E-4	0.0	0.0014030225422552616
47	15.618662790007457	1.0792481094271243E-4	4.316992437708497E-4	0.0	0.0014030225422552616
48	16.831737665003544	1.0792481094271243E-4	4.316992437708497E-4	0.0	0.0014030225422552616
49	18.058411066178415	1.0792481094271243E-4	4.316992437708497E-4	0.0	0.0014030225422552616
50	19.339262722446527	1.0792481094271243E-4	4.316992437708497E-4	0.0	0.0014030225422552616
>>END_MODULE