##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-35A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 513410 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 57 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.58609298611246 40.0 40.0 40.0 40.0 40.0 2 39.35301610798387 40.0 40.0 40.0 40.0 40.0 3 39.30475058919772 40.0 40.0 40.0 40.0 40.0 4 39.20324886542919 40.0 40.0 40.0 40.0 40.0 5 39.21726495393545 40.0 40.0 40.0 40.0 40.0 6 39.34171909390156 40.0 40.0 40.0 40.0 40.0 7 39.390467657427784 40.0 40.0 40.0 40.0 40.0 8 39.423338072885215 40.0 40.0 40.0 40.0 40.0 9 39.416598819656805 40.0 40.0 40.0 40.0 40.0 10-11 39.42161625211819 40.0 40.0 40.0 40.0 40.0 12-13 39.34477318322588 40.0 40.0 40.0 40.0 40.0 14-15 39.38455425488401 40.0 40.0 40.0 40.0 40.0 16-17 39.490360530570115 40.0 40.0 40.0 40.0 40.0 18-19 39.52534231900431 40.0 40.0 40.0 40.0 40.0 20-21 39.5103562454958 40.0 40.0 40.0 40.0 40.0 22-23 39.54564188465359 40.0 40.0 40.0 40.0 40.0 24-25 39.530223408192285 40.0 40.0 40.0 40.0 40.0 26-27 39.49559221674685 40.0 40.0 40.0 40.0 40.0 28-29 39.519140647825324 40.0 40.0 40.0 40.0 40.0 30-31 39.496857069250005 40.0 40.0 40.0 40.0 40.0 32-33 39.50504597197606 40.0 40.0 40.0 40.0 40.0 34-35 39.49977923186799 40.0 40.0 40.0 40.0 40.0 36-37 39.47917805160452 40.0 40.0 40.0 40.0 40.0 38-39 39.42283939732641 40.0 40.0 40.0 40.0 40.0 40-41 39.471586633764616 40.0 40.0 40.0 40.0 40.0 42-43 39.44918957974775 40.0 40.0 40.0 40.0 40.0 44-45 39.349203749660404 40.0 40.0 40.0 40.0 40.0 46-47 39.33517474152128 40.0 40.0 40.0 40.0 40.0 48-49 39.42609266572646 40.0 40.0 40.0 40.0 40.0 50-51 39.37036098139279 40.0 40.0 40.0 40.0 40.0 52-53 39.33157150764907 40.0 40.0 40.0 40.0 40.0 54-55 39.24830143589435 40.0 40.0 40.0 40.0 40.0 56-57 39.26577503614038 40.0 40.0 40.0 40.0 40.0 58-59 39.210125535480856 40.0 40.0 40.0 40.0 40.0 60-61 39.1289449659285 40.0 40.0 40.0 40.0 40.0 62-63 39.35881349740261 40.0 40.0 40.0 40.0 40.0 64-65 39.36533984128444 40.0 40.0 40.0 40.0 40.0 66-67 39.37796360018704 40.0 40.0 40.0 40.0 40.0 68-69 39.36806902801251 40.0 40.0 40.0 40.0 40.0 70-71 39.36714935230752 40.0 40.0 40.0 40.0 40.0 72-73 39.365461908461945 40.0 40.0 40.0 40.0 40.0 74-75 39.36929838480344 40.0 40.0 40.0 40.0 40.0 76-77 39.357399761073474 40.0 40.0 40.0 40.0 40.0 78-79 39.3417159703151 40.0 40.0 40.0 40.0 40.0 80-81 39.361528019581634 40.0 40.0 40.0 40.0 40.0 82-83 39.35058003432944 40.0 40.0 40.0 40.0 40.0 84-85 39.339565928337066 40.0 40.0 40.0 40.0 40.0 86-87 39.335440217015744 40.0 40.0 40.0 40.0 40.0 88-89 39.381450693341364 40.0 40.0 40.0 40.0 40.0 90-91 39.44099527312058 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03588614959743808 1101 2 0.06199359226540935 1101 3 0.02675162757005012 1101 4 -0.16723090273134034 1101 5 -0.02508639295079007 1101 6 -0.04187572119130323 1101 7 -0.24125759083335652 1101 8 -0.15058106982371555 1101 9 -0.003460185307979202 1101 10-11 -0.026249203053055226 1101 12-13 -0.005301294784260335 1101 14-15 0.06935438355670698 1101 16-17 -0.10044574993527533 1101 18-19 -0.06585214888492885 1101 20-21 -0.022811144895641178 1101 22-23 -0.04662816090375799 1101 24-25 -0.024628496803870803 1101 26-27 0.048622529404681814 1101 28-29 0.038426283879665846 1101 30-31 -0.06514309517998385 1101 32-33 -0.016727762712740457 1101 34-35 -0.10134824764208616 1101 36-37 -0.10048373745502914 1101 38-39 -0.04275263078318403 1101 40-41 -0.0822371774737718 1101 42-43 0.0015186314051973682 1101 44-45 -0.036340300578700635 1101 46-47 -0.06715440826490493 1101 48-49 -0.00542956667270289 1101 50-51 0.050368495347406395 1101 52-53 -0.11489758280352902 1101 54-55 -0.010965069298613628 1101 56-57 0.014243222097469754 1101 58-59 -0.026845256208410717 1101 60-61 -0.18313979245285594 1101 62-63 -0.05911333983166145 1101 64-65 -0.05247781239869198 1101 66-67 0.07031266237400047 1101 68-69 0.09837199845951972 1101 70-71 0.08722540870257944 1101 72-73 0.06322393081443778 1101 74-75 -0.19967246497280655 1101 76-77 0.13435218333851395 1101 78-79 -0.17061262406719635 1101 80-81 -0.06947448581297522 1101 82-83 -0.16863885007652613 1101 84-85 0.03769910905145224 1101 86-87 -0.5214600519932091 1101 88-89 -0.15961482513807823 1101 90-91 0.4155572826128804 1102 1 0.005081837639203002 1102 2 -0.05836863257442815 1102 3 0.10060811933698943 1102 4 -0.20923268204655443 1102 5 -0.0642074708778324 1102 6 -0.28624428794793033 1102 7 -0.041938501962029306 1102 8 -0.1312431937694214 1102 9 -0.18638969361062863 1102 10-11 -0.08326283618607988 1102 12-13 -0.2223191028468534 1102 14-15 -0.10438332152427421 1102 16-17 -0.08322317151866798 1102 18-19 -0.03785626417263188 1102 20-21 -0.09365472917205864 1102 22-23 -0.030670983752550285 1102 24-25 -0.025683163530182185 1102 26-27 -0.03419117430454577 1102 28-29 -0.05851061007214753 1102 30-31 -0.09152864992374532 1102 32-33 -0.06785472523748837 1102 34-35 -0.03384840452402216 1102 36-37 -0.1224120153581012 1102 38-39 0.029528558546402905 1102 40-41 -0.004582488003393337 1102 42-43 -0.019421055276616528 1102 44-45 0.03447582285274109 1102 46-47 -0.07622201353621705 1102 48-49 -0.01737173803502401 1102 50-51 0.07040063843977151 1102 52-53 -0.09292006877412007 1102 54-55 -0.045141272079469275 1102 56-57 -0.08021298101861163 1102 58-59 -0.04122614513940448 1102 60-61 -0.15695691158914826 1102 62-63 -0.1134990756435954 1102 64-65 -0.19864622276645605 1102 66-67 -0.16099986749362927 1102 68-69 0.03305101777837649 1102 70-71 -0.05735667674981926 1102 72-73 -0.12134360063059546 1102 74-75 -0.12172478012929844 1102 76-77 -0.24367465476094452 1102 78-79 -0.07725811512642622 1102 80-81 -0.13717244172980259 1102 82-83 -0.5291023874536833 1102 84-85 -0.3878146871332362 1102 86-87 0.08539349050390399 1102 88-89 -0.03763680316005491 1102 90-91 0.23947032609114416 1103 1 0.056875143163857445 1103 2 0.017326547598365494 1103 3 0.0524556532740732 1103 4 -0.0029519384523766234 1103 5 -0.08415525201964869 1103 6 0.06411558480000679 1103 7 0.002023452447687646 1103 8 0.025088305845663683 1103 9 0.040589458741663975 1103 10-11 0.01535603855219847 1103 12-13 -0.05057314005610891 1103 14-15 -0.03526352106118935 1103 16-17 0.08235523286570867 1103 18-19 -0.06645694948973357 1103 20-21 -0.019623341707841746 1103 22-23 0.005019290743696558 1103 24-25 -0.017358289533667914 1103 26-27 0.019478700260854964 1103 28-29 -0.07254202708863744 1103 30-31 -0.08495031498720351 1103 32-33 0.02627409209353715 1103 34-35 0.018073333811010173 1103 36-37 0.0062382968333096755 1103 38-39 0.03182439125667713 1103 40-41 -0.010614342682835343 1103 42-43 0.019852707924847834 1103 44-45 -0.04168300031228256 1103 46-47 -0.03851594511461087 1103 48-49 0.04498224807008455 1103 50-51 -0.08495141748248614 1103 52-53 0.0240647782199801 1103 54-55 -0.04672721654237222 1103 56-57 0.07826800708456716 1103 58-59 -0.061637048689973994 1103 60-61 0.18054957394301852 1103 62-63 0.009807033012840805 1103 64-65 -0.06713357122778518 1103 66-67 -0.1812927963745281 1103 68-69 0.00969902288441915 1103 70-71 0.09744404436201393 1103 72-73 0.04188986993595023 1103 74-75 0.0700092620384396 1103 76-77 -0.08714054889382084 1103 78-79 -0.057024697164692384 1103 80-81 -0.2202472120380463 1103 82-83 -0.10762973922680885 1103 84-85 0.08873090939794537 1103 86-87 0.16519352811118182 1103 88-89 0.4000921345688795 1103 90-91 0.5894703260911456 1104 1 0.005436702444740149 1104 2 0.06695478058343696 1104 3 -0.09482214421528568 1104 4 -0.1672664102421635 1104 5 -0.04055583183348688 1104 6 0.1018073940009856 1104 7 0.08723028236537544 1104 8 -0.05238702127705608 1104 9 -0.008383893475695459 1104 10-11 0.0023698506717835244 1104 12-13 0.03559743976749985 1104 14-15 0.1183705296109352 1104 16-17 0.03271136093692917 1104 18-19 0.0719623638238005 1104 20-21 -0.018644930292190054 1104 22-23 0.0013089513492943183 1104 24-25 0.019100975416428412 1104 26-27 0.06731920649345824 1104 28-29 0.020498936192090866 1104 30-31 0.007601931302701814 1104 32-33 0.00517090391333852 1104 34-35 -0.026183181754376506 1104 36-37 -0.03735161998582015 1104 38-39 -0.17425702403239995 1104 40-41 -0.057090838528750965 1104 42-43 -0.06914344728625821 1104 44-45 -0.17627153597602074 1104 46-47 -0.003810085814372144 1104 48-49 -0.069365239293262 1104 50-51 0.03977326656498548 1104 52-53 0.06259118177349876 1104 54-55 0.06266588162674225 1104 56-57 0.05107610031350163 1104 58-59 0.04825060504997225 1104 60-61 0.047067840746152 1104 62-63 -0.03917973233014749 1104 64-65 -0.03454260181075597 1104 66-67 0.11105731408678565 1104 68-69 -0.05224059771119727 1104 70-71 0.10838682407556632 1104 72-73 -0.1438200764957358 1104 74-75 0.024470523440271563 1104 76-77 -0.10820826462607158 1104 78-79 -0.26884141427196084 1104 80-81 0.0934433399309853 1104 82-83 0.43659370634050987 1104 84-85 0.39368451541859883 1104 86-87 0.11922459261804619 1104 88-89 0.18654564090190462 1104 90-91 -0.5739283667193149 1201 1 -0.040395217373216497 1201 2 -0.04676670326263377 1201 3 0.1168173165330515 1201 4 0.0457864038765905 1201 5 -0.07650788668792785 1201 6 0.12797290020797902 1201 7 -0.01943998734793695 1201 8 0.03917183750328235 1201 9 0.018743937240728314 1201 10-11 -0.06830170602905383 1201 12-13 -0.04802369987744726 1201 14-15 -0.039972678079102764 1201 16-17 0.046053650044484584 1201 18-19 0.026162521489489166 1201 20-21 0.04242768243829431 1201 22-23 -0.07382040353363095 1201 24-25 -0.09321201528401701 1201 26-27 -0.01505479946906263 1201 28-29 0.06563973204418261 1201 30-31 0.0023849660076251666 1201 32-33 0.00976221912571873 1201 34-35 0.05672888910591212 1201 36-37 -0.030316673211395084 1201 38-39 0.01655676041355747 1201 40-41 0.021604230805309044 1201 42-43 -0.011495783442050822 1201 44-45 -0.04889946646994048 1201 46-47 -0.0017301503293083442 1201 48-49 0.09193229856796847 1201 50-51 0.025482013468774767 1201 52-53 -0.03229762052704643 1201 54-55 0.09964978667607483 1201 56-57 0.08597007963913939 1201 58-59 0.1591808066185223 1201 60-61 -4.767731188906055E-4 1201 62-63 -0.07472806187990244 1201 64-65 0.0722644920847273 1201 66-67 -0.0656779703717163 1201 68-69 -0.07607299573424342 1201 70-71 0.2713713017639918 1201 72-73 -0.10260471695224993 1201 74-75 -0.12061275752868283 1201 76-77 0.061732113229027163 1201 78-79 -0.07276154271623625 1201 80-81 -0.032057918640312266 1201 82-83 0.24557970694853992 1201 84-85 0.3791750349527092 1201 86-87 0.34429837258874585 1201 88-89 0.6052203396970839 1201 90-91 0.14502588164670271 1202 1 0.08696175127705175 1202 2 0.03499539801738649 1202 3 0.023463103637716642 1202 4 0.06999499243717366 1202 5 -0.13763220962536593 1202 6 0.08360495628377862 1202 7 0.035264207871783526 1202 8 0.05884505338827495 1202 9 -0.06264970118049007 1202 10-11 0.03210056474388523 1202 12-13 0.08800372070409423 1202 14-15 0.0969672185873165 1202 16-17 0.0922774121370864 1202 18-19 0.04573060006660512 1202 20-21 0.10419536853494549 1202 22-23 -0.0217870817589656 1202 24-25 0.01980866155910377 1202 26-27 0.0790932483288671 1202 28-29 0.003120651793075524 1202 30-31 0.06041979539340048 1202 32-33 0.10721770427016963 1202 34-35 0.05377396539050494 1202 36-37 -0.1333456569686362 1202 38-39 -0.16569524898326904 1202 40-41 -0.12807153083167577 1202 42-43 -0.06352642490720939 1202 44-45 -0.06023193406417704 1202 46-47 0.043082237471729457 1202 48-49 0.0033213484466898535 1202 50-51 -0.0933303839772961 1202 52-53 -0.01740269300358932 1202 54-55 -0.1150548388796011 1202 56-57 -0.12463848363027097 1202 58-59 -0.01969355788199323 1202 60-61 -0.04231996566303309 1202 62-63 -0.07639448052295705 1202 64-65 0.006165549289192995 1202 66-67 -0.05457292062724406 1202 68-69 -0.21009625736908788 1202 70-71 -0.14701961265111407 1202 72-73 0.05290050652626377 1202 74-75 -0.10220303179306711 1202 76-77 -0.11088742406840879 1202 78-79 0.1557310066545412 1202 80-81 0.08363098356221599 1202 82-83 -0.09387604130073868 1202 84-85 -0.258132774842629 1202 86-87 -0.00164903863380772 1202 88-89 -0.6310595094859934 1202 90-91 -0.3670514130392917 1203 1 0.03468207998718498 1203 2 0.12328748962487879 1203 3 0.10856598807293949 1203 4 -0.2368055903388182 1203 5 -0.09055698792958111 1203 6 -0.0996654724236734 1203 7 0.010731403778585502 1203 8 0.03577301325167781 1203 9 -0.02000265922750799 1203 10-11 0.0796375331615593 1203 12-13 0.10855288382401085 1203 14-15 -0.0683486238184301 1203 16-17 -0.0578552802956267 1203 18-19 0.0069897633012416804 1203 20-21 0.05455329394420971 1203 22-23 -0.007645012904212933 1203 24-25 0.034781492114312584 1203 26-27 0.00733130696591644 1203 28-29 -0.03714786874366638 1203 30-31 0.024611958509495935 1203 32-33 -0.013942690711417072 1203 34-35 0.0513095828859349 1203 36-37 0.021113532212446273 1203 38-39 -0.06907168412911346 1203 40-41 -0.02397809277572094 1203 42-43 -0.021951051715724645 1203 44-45 -0.0722372911060063 1203 46-47 -0.07880493989677717 1203 48-49 -0.02879903885156665 1203 50-51 -0.09905252949894106 1203 52-53 -0.02999286129519163 1203 54-55 -0.10554512686507422 1203 56-57 0.004565111097775798 1203 58-59 0.0165849541923464 1203 60-61 0.004787182899825382 1203 62-63 0.02882578620589271 1203 64-65 0.015099235266532673 1203 66-67 0.13998085891448397 1203 68-69 -0.10533174535044765 1203 70-71 -0.18114012353233733 1203 72-73 -0.07910636217419409 1203 74-75 -0.16372336780840158 1203 76-77 -0.04068270369284477 1203 78-79 0.02144466183319338 1203 80-81 0.12006687433407137 1203 82-83 -0.07870677480195098 1203 84-85 0.2684816661584648 1203 86-87 0.40746744186935047 1203 88-89 0.16077589525264102 1203 90-91 0.5894703260911456 1204 1 -0.09289459315107962 1204 2 -0.16207176074178165 1204 3 -0.32790702842007136 1204 4 -0.4300287915979055 1204 5 -0.37160630611401047 1204 6 -0.239538184866376 1204 7 -0.17577129016836324 1204 8 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0.05715102551310025 2401 30-31 -0.07187840470370332 2401 32-33 -0.025948435346521137 2401 34-35 -0.06167475209148421 2401 36-37 -0.04424423345924566 2401 38-39 0.11370019290103528 2401 40-41 -0.027450776148029377 2401 42-43 0.026860526226798243 2401 44-45 0.03170603835977204 2401 46-47 -0.03469455036968583 2401 48-49 0.021275435957633704 2401 50-51 0.047428885295666134 2401 52-53 0.023557274040214793 2401 54-55 -0.03925269385570118 2401 56-57 0.022074758309123865 2401 58-59 -0.02921787494523187 2401 60-61 0.04834673473737183 2401 62-63 8.181315052979699E-6 2401 64-65 -0.08962784475694008 2401 66-67 0.07474379488592575 2401 68-69 -0.04103088472402305 2401 70-71 0.20228193191125143 2401 72-73 0.07638228622975163 2401 74-75 -0.004023005850868344 2401 76-77 0.09101632196358622 2401 78-79 0.07499007304342342 2401 80-81 0.06368359353361086 2401 82-83 -0.04057905525207417 2401 84-85 -0.057157987511011754 2401 86-87 -0.08909159126653066 2401 88-89 0.2528393873161292 2401 90-91 0.5894703260911456 2402 1 0.11306414660796804 2402 2 0.08374794125334262 2402 3 0.1749220792927062 2402 4 0.22124907799837246 2402 5 0.21730974696956906 2402 6 0.09725877187214849 2402 7 0.07087892711586363 2402 8 -0.016851290453459455 2402 9 0.14778749058985596 2402 10-11 0.12261076367559554 2402 12-13 0.1107013247705595 2402 14-15 0.04010720447099203 2402 16-17 0.054668027568169464 2402 18-19 0.11210896427532191 2402 20-21 0.0187885901378948 2402 22-23 0.021669956479392738 2402 24-25 0.043025270311680686 2402 26-27 -0.03332437191789239 2402 28-29 0.038075504411558825 2402 30-31 0.03401157146660694 2402 32-33 0.05018049856340667 2402 34-35 0.005330641802174796 2402 36-37 0.06391928983821771 2402 38-39 -0.39446655829848964 2402 40-41 0.07129133528243159 2402 42-43 0.06981650816181428 2402 44-45 0.07637495675507466 2402 46-47 0.020533433021185488 2402 48-49 0.01833431061172064 2402 50-51 0.13394840914985195 2402 52-53 0.14609343202006642 2402 54-55 0.15620982391448734 2402 56-57 -0.0025899866136995797 2402 58-59 0.16351866065630816 2402 60-61 0.07083763103066332 2402 62-63 0.032329575897641405 2402 64-65 0.09889739560686905 2402 66-67 0.10414691097640372 2402 68-69 0.12516611776815267 2402 70-71 0.1413239851910717 2402 72-73 -0.02404488424683393 2402 74-75 0.23105939616094417 2402 76-77 0.18938939180628722 2402 78-79 0.16788910164186177 2402 80-81 0.15227317728181333 2402 82-83 -0.028867607625500113 2402 84-85 0.007958428639881276 2402 86-87 0.05984868642467234 2402 88-89 -0.16068875121200676 2402 90-91 0.06265040271949829 2403 1 -0.06979274246168643 2403 2 0.06695171774470765 2403 3 -0.040505762162908354 2403 4 0.15075426085643073 2403 5 0.10147412579214432 2403 6 0.06426005399985257 2403 7 0.01191507797449276 2403 8 0.04094380052851676 2403 9 0.07890088467523526 2403 10-11 0.03601513412985469 2403 12-13 0.009279997972925003 2403 14-15 0.03515166987516949 2403 16-17 -0.06536226664331934 2403 18-19 0.01660155376421102 2403 20-21 0.039712867465510726 2403 22-23 -0.023357620369374388 2403 24-25 0.07402073669877751 2403 26-27 0.1302865765410388 2403 28-29 -0.015797234187495235 2403 30-31 0.021230032235493468 2403 32-33 -0.003714857749699263 2403 34-35 0.05134260120696155 2403 36-37 -0.09522336988882785 2403 38-39 0.06880116235677747 2403 40-41 -0.022592320677929933 2403 42-43 0.03709374042594504 2403 44-45 -0.07929086310535638 2403 46-47 0.011729832546045316 2403 48-49 -0.0395178193555239 2403 50-51 0.03529877253877345 2403 52-53 0.02584415810403584 2403 54-55 -0.06185566239243201 2403 56-57 0.18904050644628967 2403 58-59 -0.0659119844372853 2403 60-61 -0.04355434296716254 2403 62-63 -0.05338293107176639 2403 64-65 0.07788553086037808 2403 66-67 0.04714564948069011 2403 68-69 -0.06261750612378592 2403 70-71 0.02303378948592183 2403 72-73 0.2044057820283527 2403 74-75 0.11876133328514271 2403 76-77 -0.01922911381820569 2403 78-79 0.12424670230724644 2403 80-81 0.17641129442559134 2403 82-83 0.012801698890314128 2403 84-85 0.18467319749337463 2403 86-87 0.48459555106278884 2403 88-89 0.3979052667103673 2403 90-91 -0.8207860841652632 2404 1 -0.008146113616511741 2404 2 0.08773805382603683 2404 3 0.07100742459922316 2404 4 0.0952850282473463 2404 5 0.15206682889340328 2404 6 0.06514794716358807 2404 7 0.03645603204666514 2404 8 0.11884438058429936 2404 9 0.0712264592677272 2404 10-11 0.09959241438919975 2404 12-13 0.006786670077076451 2404 14-15 0.09397287124968301 2404 16-17 0.10407218326729151 2404 18-19 0.029150787956353952 2404 20-21 0.1129847021521897 2404 22-23 0.07617761229820985 2404 24-25 0.051006064092177894 2404 26-27 0.042151134977665095 2404 28-29 0.006286527175724643 2404 30-31 0.048011367895242074 2404 32-33 0.03402202114153852 2404 34-35 0.018405878491151384 2404 36-37 0.13458424387674484 2404 38-39 0.1074748410089228 2404 40-41 -0.021855367907498646 2404 42-43 0.02436012179232705 2404 44-45 0.02103866145036193 2404 46-47 0.05992949973708761 2404 48-49 0.09229331249831318 2404 50-51 0.111019872270127 2404 52-53 0.014299805930953369 2404 54-55 0.08157341411707364 2404 56-57 0.062499967767358555 2404 58-59 -0.028250552112709215 2404 60-61 0.02893894793722751 2404 62-63 0.09489089585170518 2404 64-65 0.09989244456566126 2404 66-67 0.01822248047612618 2404 68-69 0.04334174104168653 2404 70-71 -0.08578153258078913 2404 72-73 0.019896533732357113 2404 74-75 -0.05224169904368381 2404 76-77 0.1044240990994183 2404 78-79 -0.06594308651648362 2404 80-81 -0.05000467813574261 2404 82-83 -0.25977492806448055 2404 84-85 -0.11857628028014489 2404 86-87 0.24280257426942597 2404 88-89 0.6052203396970839 2404 90-91 0.5894703260911456 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 9.0 31 288.0 32 2131.0 33 3702.0 34 4975.0 35 6227.0 36 8668.0 37 13028.0 38 31920.0 39 232464.0 40 209998.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 31.157164839017547 13.31372587211001 23.2210124461931 32.30809684267934 2 30.544114839247655 17.03476116070559 23.941013961353974 28.480110038692786 3 27.93918287210172 18.830442844677137 19.856722139117426 33.373652144103716 4 28.171831479714072 19.99922089558053 19.3321127364095 32.49683488829591 5 28.617868759860542 20.31670594651448 19.351979899105977 31.713445394519002 6 28.781870240158934 22.65694084649695 22.092479694591066 26.468709218753045 7 27.732806139342824 22.287645351668257 21.49471182875285 28.48483668023607 8 26.99694201515358 22.901774410315344 21.606708089051637 28.49457548547944 9 26.481564441674294 23.10317290274829 22.600650552190256 27.814612103387155 10-11 26.993922985528133 21.73146218421924 22.12617596073314 29.148438869519488 12-13 27.306733409945267 21.597748388227732 22.046122981632614 29.049395220194384 14-15 27.592956896047994 21.314641319802888 21.83157710212111 29.26082468202801 16-17 27.891061724547633 21.257182368866985 21.46364504002649 29.388110866558893 18-19 27.792797179642005 21.554215928789855 21.60427338774079 29.04871350382735 20-21 27.750628152938194 21.54963869032547 21.74840770534271 28.951325451393622 22-23 27.760951286496173 20.954013361640794 21.709160320211918 29.575875031651115 24-25 27.871194561851155 21.296527142050216 21.641475623770475 29.190802672328157 26-27 28.000038955220973 21.465884965232465 21.64167039987534 28.892405679671217 28-29 28.041818429715043 21.201671178979765 21.60495510410783 29.151555287197368 30-31 27.930970676171835 21.413406569862 21.83305577468081 28.82256697928536 32-33 27.943457160435344 21.427841343536176 21.855362292062654 28.77333920396582 34-35 28.101810900104812 21.17722966888421 21.79671925812831 28.92424017288267 36-37 28.042980626234016 21.481887533689644 21.788456950852456 28.686674889223884 38-39 28.262495940265612 21.49184473292914 21.83405569799934 28.411603628805903 40-41 28.151916602976552 21.238624803747637 21.861423774359476 28.748034818916334 42-43 28.105880692788247 21.455411027160423 21.989999573379308 28.448708706672022 44-45 28.24313905222911 21.5370148569674 21.921844291395693 28.298001799407796 46-47 28.226436453621368 21.322384024175175 21.841977237537012 28.609202284666445 48-49 28.31839890613329 21.441222911836192 21.95275644191631 28.287621740114204 50-51 28.385261205878244 21.64369816209625 21.895949498926726 28.075091133098777 52-53 28.368811324196535 21.34338354444369 21.86440204040057 28.423403090959205 54-55 28.369480072699858 21.618819005659883 21.88548791441932 28.12621300722094 56-57 28.497623567805014 21.59152042753549 21.83649968655104 28.074356318108457 58-59 28.35136211954904 21.447457728546166 21.813912690176267 28.387267461728527 60-61 28.68437032688468 21.520426717982275 21.88914550430536 27.90605745082768 62-63 28.710746461328178 21.601582345422102 21.824868062548926 27.86280313070079 64-65 28.513640658100382 21.43599052479417 21.695561089061847 28.354807728043596 66-67 28.61168786319393 21.718304334664957 21.630903300348326 28.03910450179279 68-69 28.737745098039213 21.735378311629997 21.64954722346954 27.877329366861247 70-71 28.726332255060406 21.676139385150965 21.625035992871467 27.972492366917166 72-73 28.784033259197884 21.759772069832685 21.642899713633653 27.813294957335778 74-75 28.8985794603069 21.8083967630271 21.470141658734523 27.822882117931474 76-77 28.859134248737494 21.670720093488086 21.447623528340472 28.022522129433952 78-79 29.04019249621246 21.66117101862579 21.385794492469476 27.91284199269227 80-81 29.102230115383996 21.89781802814792 21.037653591240016 27.96229826522807 82-83 29.267823502878827 21.373610337233675 21.13879381251824 28.21977234736926 84-85 29.62467990446711 21.337115562031713 20.646485200162097 28.391719333339083 86-87 30.086891367662876 20.86963877844514 20.154204986283492 28.889264867608492 88-89 28.93324917153227 20.715638314659934 20.226447845983905 30.124664667823893 90-91 25.68034455014435 22.845378389890673 22.89270670642245 28.581570353542524 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 3.0 10 5.0 11 4.0 12 1.0 13 1.5 14 6.0 15 9.0 16 11.5 17 16.5 18 26.5 19 44.0 20 65.5 21 95.0 22 141.5 23 209.0 24 273.5 25 336.5 26 437.5 27 576.5 28 728.5 29 897.0 30 1096.5 31 1339.5 32 1635.0 33 1964.5 34 2408.0 35 2923.0 36 3484.0 37 4126.5 38 4922.0 39 5979.5 40 7099.0 41 8271.0 42 9688.0 43 11218.0 44 12887.0 45 14796.0 46 16695.5 47 18514.5 48 20421.0 49 22583.5 50 24525.5 51 26361.0 52 28325.0 53 30008.0 54 31450.0 55 32545.0 56 33422.5 57 34383.0 58 35179.0 59 35768.0 60 35969.5 61 35697.5 62 35318.5 63 34388.0 64 32770.5 65 30623.5 66 27983.0 67 25539.5 68 22981.0 69 19965.0 70 16986.5 71 14269.5 72 11734.5 73 9289.0 74 7212.0 75 5564.5 76 4227.0 77 3116.0 78 2256.5 79 1620.5 80 1131.5 81 779.5 82 535.5 83 357.5 84 231.0 85 152.0 86 111.5 87 81.5 88 51.5 89 28.5 90 19.5 91 17.5 92 13.0 93 9.0 94 5.5 95 4.0 96 2.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.02648955026197386 3 3.895522097349097E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.07403206189661019 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 1.8079782464057394E-4 64-65 0.0 66-67 2.1317280000341076E-4 68-69 2.3387162786346572E-4 70-71 0.0 72-73 2.9072640900554124E-4 74-75 9.880772017653646E-4 76-77 0.0 78-79 0.0 80-81 5.349252709396497E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.00473260766682442 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 3007.0 32-33 7010.0 34-35 8454.0 36-37 9897.0 38-39 11543.0 40-41 12908.0 42-43 13965.0 44-45 15153.0 46-47 16220.0 48-49 17145.0 50-51 18094.0 52-53 18373.0 54-55 19395.0 56-57 19697.0 58-59 20136.0 60-61 20602.0 62-63 20977.0 64-65 21120.0 66-67 20659.0 68-69 21074.0 70-71 20897.0 72-73 20270.0 74-75 20046.0 76-77 19806.0 78-79 18832.0 80-81 18323.0 82-83 17578.0 84-85 16777.0 86-87 16231.0 88-89 15026.0 90-91 14195.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.75453775450221 #Duplication Level Percentage of deduplicated Percentage of total 1 99.77233540990025 99.52743199501751 2 0.2142601197487744 0.4274683840952656 3 0.010619215273714445 0.03177944732834802 4 0.0020207938662311765 0.008063334320920976 5 0.0 0.0 6 5.36792118104611E-4 0.0032128469767071367 7 0.0 0.0 8 0.0 0.0 9 2.2766909291753855E-4 0.002043992261247828 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002921641573011823 0.0 0.0 0.0 0.0 2 0.002921641573011823 0.0 0.0 0.0 1.9477610486745486E-4 3 0.002921641573011823 0.0 0.0 0.0 1.9477610486745486E-4 4 0.002921641573011823 0.0 0.0 0.0 3.895522097349097E-4 5 0.002921641573011823 0.0 0.0 0.0 3.895522097349097E-4 6 0.0031164176778792777 0.0 0.0 0.0 3.895522097349097E-4 7 0.0033111937827467325 0.0 0.0 0.0 3.895522097349097E-4 8 0.00681716367036092 0.0 0.0 0.0 3.895522097349097E-4 9 0.00759626808983074 0.0 0.0 0.0 5.843283146023646E-4 10-11 0.00759626808983074 0.0 9.738805243372743E-5 0.0 5.843283146023646E-4 12-13 0.00759626808983074 1.9477610486745486E-4 1.9477610486745486E-4 0.0 5.843283146023646E-4 14-15 0.007791044194698195 1.9477610486745486E-4 1.9477610486745486E-4 0.0 5.843283146023646E-4 16-17 0.00798582029956565 1.9477610486745486E-4 1.9477610486745486E-4 0.0 6.81716367036092E-4 18-19 0.00798582029956565 1.9477610486745486E-4 1.9477610486745486E-4 0.0 7.791044194698194E-4 20-21 0.00798582029956565 1.9477610486745486E-4 1.9477610486745486E-4 0.0 8.764924719035468E-4 22-23 0.00798582029956565 1.9477610486745486E-4 1.9477610486745486E-4 0.0 9.738805243372743E-4 24-25 0.00798582029956565 1.9477610486745486E-4 3.895522097349097E-4 0.0 9.738805243372743E-4 26-27 0.008180596404433103 1.9477610486745486E-4 3.895522097349097E-4 0.0 9.738805243372743E-4 28-29 0.008180596404433103 1.9477610486745486E-4 3.895522097349097E-4 0.0 9.738805243372743E-4 30-31 0.008180596404433103 1.9477610486745486E-4 3.895522097349097E-4 0.0 9.738805243372743E-4 32-33 0.008180596404433103 1.9477610486745486E-4 3.895522097349097E-4 0.0 0.0011686566292047292 34-35 0.008180596404433103 1.9477610486745486E-4 3.895522097349097E-4 0.0 0.0011686566292047292 36-37 0.008180596404433103 1.9477610486745486E-4 3.895522097349097E-4 0.0 0.0011686566292047292 38-39 0.008375372509300559 1.9477610486745486E-4 3.895522097349097E-4 0.0 0.0012660446816384566 40-41 0.008375372509300559 1.9477610486745486E-4 3.895522097349097E-4 0.0 0.0015582088389396388 42-43 0.00876492471903547 1.9477610486745486E-4 3.895522097349097E-4 0.0 0.0015582088389396388 44-45 0.009349253033637834 1.9477610486745486E-4 3.895522097349097E-4 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