##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-34C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 602322 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.580390555217974 40.0 40.0 40.0 40.0 40.0 2 39.32709746613937 40.0 40.0 40.0 40.0 40.0 3 39.27945517513888 40.0 40.0 40.0 40.0 40.0 4 39.176035409631396 40.0 40.0 40.0 40.0 40.0 5 39.25424606771793 40.0 40.0 40.0 40.0 40.0 6 39.338765643625834 40.0 40.0 40.0 40.0 40.0 7 39.39800970245151 40.0 40.0 40.0 40.0 40.0 8 39.39017336242077 40.0 40.0 40.0 40.0 40.0 9 39.400008633255965 40.0 40.0 40.0 40.0 40.0 10-11 39.402797839029624 40.0 40.0 40.0 40.0 40.0 12-13 39.3247332821979 40.0 40.0 40.0 40.0 40.0 14-15 39.36503066466109 40.0 40.0 40.0 40.0 40.0 16-17 39.466720458492304 40.0 40.0 40.0 40.0 40.0 18-19 39.50508531981232 40.0 40.0 40.0 40.0 40.0 20-21 39.47903613017621 40.0 40.0 40.0 40.0 40.0 22-23 39.52508126882299 40.0 40.0 40.0 40.0 40.0 24-25 39.49574148047058 40.0 40.0 40.0 40.0 40.0 26-27 39.47389270191027 40.0 40.0 40.0 40.0 40.0 28-29 39.497239018332394 40.0 40.0 40.0 40.0 40.0 30-31 39.4759553223425 40.0 40.0 40.0 40.0 40.0 32-33 39.47857116319742 40.0 40.0 40.0 40.0 40.0 34-35 39.47591692870546 40.0 40.0 40.0 40.0 40.0 36-37 39.45930720038338 40.0 40.0 40.0 40.0 40.0 38-39 39.393778290914355 40.0 40.0 40.0 40.0 40.0 40-41 39.45313484515988 40.0 40.0 40.0 40.0 40.0 42-43 39.430212126353005 40.0 40.0 40.0 40.0 40.0 44-45 39.33847151062229 40.0 40.0 40.0 40.0 40.0 46-47 39.319750960172314 40.0 40.0 40.0 40.0 40.0 48-49 39.40793954196279 40.0 40.0 40.0 40.0 40.0 50-51 39.36018030942688 40.0 40.0 40.0 40.0 40.0 52-53 39.315008830693564 40.0 40.0 40.0 40.0 40.0 54-55 39.22985000208526 40.0 40.0 40.0 40.0 40.0 56-57 39.24072586530551 40.0 40.0 40.0 40.0 40.0 58-59 39.194851082957726 40.0 40.0 40.0 40.0 40.0 60-61 39.10949961183752 40.0 40.0 40.0 40.0 40.0 62-63 39.36756555544238 40.0 40.0 40.0 40.0 40.0 64-65 39.36938853781895 40.0 40.0 40.0 40.0 40.0 66-67 39.38109096745431 40.0 40.0 40.0 40.0 40.0 68-69 39.3762415463335 40.0 40.0 40.0 40.0 40.0 70-71 39.369690449949005 40.0 40.0 40.0 40.0 40.0 72-73 39.370951989309816 40.0 40.0 40.0 40.0 40.0 74-75 39.35582544502744 40.0 40.0 40.0 40.0 40.0 76-77 39.3668282313412 40.0 40.0 40.0 40.0 40.0 78-79 39.37037666775996 40.0 40.0 40.0 40.0 40.0 80-81 39.37234563545577 40.0 40.0 40.0 40.0 40.0 82-83 39.33988629622772 40.0 40.0 40.0 40.0 40.0 84-85 39.348351131321294 40.0 40.0 40.0 40.0 40.0 86-87 39.347322406011145 40.0 40.0 40.0 40.0 40.0 88-89 39.36853064181908 40.0 40.0 40.0 40.0 40.0 90-91 39.479461618488656 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.005836931785623278 1101 2 0.15459894447867129 1101 3 0.11567967046260463 1101 4 -0.14928971541586833 1101 5 -0.08065975183453844 1101 6 -0.05541235095120811 1101 7 -0.039464504975541104 1101 8 -8.722342986544618E-4 1101 9 -0.02366236624464335 1101 10-11 -0.08385662750730205 1101 12-13 -0.023692291826222345 1101 14-15 -0.10816143833304892 1101 16-17 0.047122986914565956 1101 18-19 0.012725203781897676 1101 20-21 0.03496619504630161 1101 22-23 -0.08961121622611046 1101 24-25 -0.0620748360637009 1101 26-27 0.0012501674305553934 1101 28-29 -0.04560765150199586 1101 30-31 -0.04059309946230627 1101 32-33 0.04803301137872751 1101 34-35 -0.056496789462691765 1101 36-37 -0.10398600752940013 1101 38-39 -0.15408449266467983 1101 40-41 -0.13495531413526862 1101 42-43 -0.03799775590977106 1101 44-45 -0.04166143734624228 1101 46-47 0.03910044254377709 1101 48-49 -0.10399901859121741 1101 50-51 0.014251181478591946 1101 52-53 -0.03143582470026729 1101 54-55 -0.11479474364946185 1101 56-57 -0.09652928974978892 1101 58-59 0.02750867487901587 1101 60-61 0.10508373412032057 1101 62-63 0.1442622298278735 1101 64-65 0.086621375300858 1101 66-67 0.0012080387569497475 1101 68-69 0.06492580705867113 1101 70-71 -0.023802877259292643 1101 72-73 0.17615247413066726 1101 74-75 0.07785851256882381 1101 76-77 -0.10001528363565626 1101 78-79 0.213444865149647 1101 80-81 0.15373429312547415 1101 82-83 0.2030988891052985 1101 84-85 0.11451546519989364 1101 86-87 -0.24990744314111168 1101 88-89 -0.03989008531974747 1101 90-91 0.20777453405121094 1102 1 0.12624431804109548 1102 2 0.11221840002787786 1102 3 0.060782468863514794 1102 4 -0.052511683814877586 1102 5 -0.12603858297864434 1102 6 0.07517969169109051 1102 7 0.08315590241071646 1102 8 0.058593785118823405 1102 9 -0.08814932607711512 1102 10-11 -0.0784311563479605 1102 12-13 0.012251454604388812 1102 14-15 -0.062033035515611346 1102 16-17 0.014213221066476933 1102 18-19 -0.01284351885278312 1102 20-21 -0.02701029457867321 1102 22-23 0.05791162786297832 1102 24-25 -0.015137371757589335 1102 26-27 -0.13557640785975877 1102 28-29 0.0191767574071946 1102 30-31 -0.0631516374406047 1102 32-33 0.05496152380312225 1102 34-35 -0.03353414349188455 1102 36-37 0.06875604329060536 1102 38-39 0.017008755392438957 1102 40-41 0.0431047264873996 1102 42-43 0.07515162537181652 1102 44-45 -0.005619265159388931 1102 46-47 0.015865598079429333 1102 48-49 0.021105742625806556 1102 50-51 0.07872154629527728 1102 52-53 -6.267582874457389E-4 1102 54-55 0.1016419314556245 1102 56-57 0.030484536094043335 1102 58-59 0.1004166270231508 1102 60-61 0.07781620745992512 1102 62-63 0.12786937350031025 1102 64-65 0.15010218790415308 1102 66-67 -0.03176619601740782 1102 68-69 0.06149349188318354 1102 70-71 -0.10203214227369983 1102 72-73 -0.10011977460099075 1102 74-75 -0.005444326894632923 1102 76-77 0.08870804904599083 1102 78-79 0.016667120367699795 1102 80-81 -0.11632147470213994 1102 82-83 0.1444012584719374 1102 84-85 0.04042578537913499 1102 86-87 0.08948692872078823 1102 88-89 0.026720935776474164 1102 90-91 0.5215000242472883 1103 1 0.01092459523344047 1103 2 0.10637520519341592 1103 3 -6.984201602975304E-4 1103 4 -0.1793484917826973 1103 5 -0.019562101710143054 1103 6 0.027408089311492745 1103 7 -0.03493837101022734 1103 8 -0.08062268529083383 1103 9 0.03336898508121777 1103 10-11 0.10688211537409842 1103 12-13 -0.11403000538428643 1103 14-15 0.09345491175479026 1103 16-17 -0.002760285696943754 1103 18-19 -0.05027295098211226 1103 20-21 0.053747956097083716 1103 22-23 0.007601917373690981 1103 24-25 -0.07282569383314552 1103 26-27 0.02679902196936723 1103 28-29 -0.09312321515379551 1103 30-31 -0.04955546372250552 1103 32-33 0.020262189518156504 1103 34-35 0.009978573057338735 1103 36-37 -0.023385908074054385 1103 38-39 0.15023458481857688 1103 40-41 0.012668011276893765 1103 42-43 0.09859823247683153 1103 44-45 0.02326171827058232 1103 46-47 0.015553053378283721 1103 48-49 0.04979705287818348 1103 50-51 0.03420118072942557 1103 52-53 -0.09817947887494682 1103 54-55 -0.03434852059658766 1103 56-57 0.0999317060037086 1103 58-59 -0.15757476516490954 1103 60-61 0.02753680642372558 1103 62-63 0.08213283338238142 1103 64-65 0.004079076862502973 1103 66-67 0.11759252332542758 1103 68-69 -0.1505328217853048 1103 70-71 -0.013204067834500677 1103 72-73 0.06089306609009526 1103 74-75 -0.06882156297282904 1103 76-77 0.09176015165586193 1103 78-79 -0.017214184568508983 1103 80-81 0.03300435595953388 1103 82-83 -0.24813188631244287 1103 84-85 0.23832402128436314 1103 86-87 -0.2374889939073057 1103 88-89 0.022018129703802458 1103 90-91 -0.8579466160689151 1104 1 0.09919936509816552 1104 2 0.005981193491351178 1104 3 0.046956951693118754 1104 4 -0.21766600586137486 1104 5 0.08572111146484929 1104 6 -0.051722889300819475 1104 7 0.04626784450135801 1104 8 0.05404531576024141 1104 9 -0.058928769625779864 1104 10-11 0.005149469631206216 1104 12-13 -0.11300584410225412 1104 14-15 -0.041270459326604225 1104 16-17 -0.08949102264685393 1104 18-19 -0.10362707918075387 1104 20-21 -0.02367112914132008 1104 22-23 -0.03094790991418961 1104 24-25 -0.0958905708381721 1104 26-27 -0.016945980286479312 1104 28-29 -0.04198314516235513 1104 30-31 -0.005023571297691376 1104 32-33 0.06145363216530342 1104 34-35 0.018876714747065648 1104 36-37 -0.1298355948716008 1104 38-39 -0.07242870818632241 1104 40-41 -0.12211751873982024 1104 42-43 -0.06672048150734611 1104 44-45 -0.12625380782844786 1104 46-47 -0.028062481575972242 1104 48-49 -0.06556001315385629 1104 50-51 -0.19954534824159254 1104 52-53 -0.03465945852037322 1104 54-55 -0.19926413036374413 1104 56-57 -0.04240731949962395 1104 58-59 -0.047721731905539855 1104 60-61 -0.06732974490086718 1104 62-63 0.019881347729921117 1104 64-65 -0.00954953577315365 1104 66-67 -0.022982316566960037 1104 68-69 -0.2814443603451906 1104 70-71 0.05103167266113928 1104 72-73 -0.0630987393495488 1104 74-75 -0.0021330455726342734 1104 76-77 0.0014650500238673203 1104 78-79 -0.002019898895262884 1104 80-81 0.013710251231643156 1104 82-83 0.1030771452479442 1104 84-85 0.16542817810400123 1104 86-87 0.25036623168791294 1104 88-89 0.1206833913827623 1104 90-91 0.5215000242472883 1201 1 6.971243833717722E-4 1201 2 -0.026721394307735125 1201 3 -0.003524993651566888 1201 4 -0.004821099567159592 1201 5 -0.004975217665837306 1201 6 -0.10472781678250698 1201 7 -0.07465130158767153 1201 8 -0.17113850648800621 1201 9 -0.12114467277672247 1201 10-11 -0.022518305196129518 1201 12-13 0.019341992893586735 1201 14-15 -0.007823522101467972 1201 16-17 -0.04509768833778338 1201 18-19 -0.0852023400166857 1201 20-21 -0.0570441889889679 1201 22-23 0.009235807790602735 1201 24-25 -0.0053266634218616105 1201 26-27 0.018320013120309397 1201 28-29 0.00277818212138925 1201 30-31 -0.04415209702583667 1201 32-33 -0.10788722250463678 1201 34-35 0.04135138848693032 1201 36-37 -0.03560630962235933 1201 38-39 0.0559647710879716 1201 40-41 -0.09541545207775926 1201 42-43 -0.02047949659386461 1201 44-45 0.026147860975392234 1201 46-47 0.04747497948454793 1201 48-49 0.058459718174098896 1201 50-51 -0.06368444381676142 1201 52-53 0.027921023920541188 1201 54-55 0.02876541735304272 1201 56-57 -0.04062776777309551 1201 58-59 -0.06962614488989516 1201 60-61 0.1655039608032567 1201 62-63 0.06171878774475914 1201 64-65 -0.10198082141680942 1201 66-67 -0.0470269727056305 1201 68-69 -0.013159601373388341 1201 70-71 0.08468416303911397 1201 72-73 -0.1466091491840089 1201 74-75 0.0036998101956839946 1201 76-77 -0.2263466315394851 1201 78-79 -0.13605909252549964 1201 80-81 -0.11700216164010868 1201 82-83 0.014100395433153778 1201 84-85 -0.18268926235630545 1201 86-87 -0.1429487856401579 1201 88-89 -0.17419666590330252 1201 90-91 0.36765387040113495 1202 1 0.1506381602840321 1202 2 0.1267056129789168 1202 3 0.10577404251884559 1202 4 0.031004173033728932 1202 5 0.08138142349006472 1202 6 0.16865395587544185 1202 7 0.03169987544641373 1202 8 0.027985159038571794 1202 9 -0.04235341952158933 1202 10-11 0.1055192104959346 1202 12-13 -0.05439114354692265 1202 14-15 0.05977942299102068 1202 16-17 -0.05320533845415554 1202 18-19 0.04710410730122305 1202 20-21 0.06233165613430458 1202 22-23 -0.03641520082403815 1202 24-25 -0.0678140838376251 1202 26-27 0.03398959283251202 1202 28-29 -0.04018641521086863 1202 30-31 0.019936518580117024 1202 32-33 0.004021239519502728 1202 34-35 -0.05952405934662863 1202 36-37 -0.08097785031709748 1202 38-39 -0.08022849898326001 1202 40-41 -0.22498782416614205 1202 42-43 -0.1636930849095819 1202 44-45 -0.1253157718111666 1202 46-47 -0.1487766317807484 1202 48-49 -0.08508315217943618 1202 50-51 -0.03688635556348885 1202 52-53 -0.11266369400863141 1202 54-55 -0.14656846246717237 1202 56-57 -0.0647507974818069 1202 58-59 0.0038579845141555325 1202 60-61 -0.16015551804355965 1202 62-63 -0.10503667961464913 1202 64-65 -0.16569062074702856 1202 66-67 -0.12122980824366181 1202 68-69 0.016640034788984792 1202 70-71 -0.13176074575469698 1202 72-73 -0.10780367267499713 1202 74-75 -0.2857359807837412 1202 76-77 -0.05664439877941163 1202 78-79 -0.12223688482487916 1202 80-81 -0.44973131050068105 1202 82-83 -0.37280904843065343 1202 84-85 -0.32392811627710216 1202 86-87 -0.3231119695704976 1202 88-89 0.16202150368861368 1202 90-91 0.14940700099147364 1203 1 0.05546610988172773 1203 2 0.031127103237857057 1203 3 -0.06478275630430375 1203 4 -0.10458366373785566 1203 5 -0.2195613401036951 1203 6 -0.15581365136774394 1203 7 0.04006324740348077 1203 8 -0.006845176152424415 1203 9 0.11670811700911088 1203 10-11 -0.1242702886539675 1203 12-13 -0.028452442864178806 1203 14-15 -0.08577878060674493 1203 16-17 -0.014502872410169232 1203 18-19 -0.06810423507482 1203 20-21 1.9317530299645114E-4 1203 22-23 -0.0344387586420396 1203 24-25 0.004349272439803542 1203 26-27 -0.003444370142673847 1203 28-29 -0.018251069836516365 1203 30-31 -0.05561646632200734 1203 32-33 0.0022378871311872217 1203 34-35 0.017962470152049548 1203 36-37 -0.09345699691427001 1203 38-39 0.09360884277189996 1203 40-41 0.011571076284859316 1203 42-43 -0.010010700434307296 1203 44-45 0.03429950611403143 1203 46-47 0.09145777895113838 1203 48-49 0.02615213959588658 1203 50-51 -0.07760903827004739 1203 52-53 0.04879721366017975 1203 54-55 -0.10468835681226096 1203 56-57 -0.03822947527454801 1203 58-59 -0.083530234177843 1203 60-61 0.015695325377315328 1203 62-63 -0.002618607604908618 1203 64-65 -0.08471616928523673 1203 66-67 -0.16719753793427827 1203 68-69 -0.16010804228825748 1203 70-71 -0.23149722869737133 1203 72-73 -0.08231917803608013 1203 74-75 -0.07217946367607908 1203 76-77 -0.1881596538540009 1203 78-79 -0.31130167528869634 1203 80-81 0.0848981435516265 1203 82-83 -0.002109120541064158 1203 84-85 -0.008707942185957052 1203 86-87 -0.037497198263338305 1203 88-89 0.03359119280120382 1203 90-91 0.36765387040113495 1204 1 -0.046799656830408765 1204 2 -0.05175932698525543 1204 3 -0.12254134609936074 1204 4 -0.07664075291824446 1204 5 -0.2895165955428922 1204 6 0.10873194754488935 1204 7 -0.024068540764609736 1204 8 -0.12972717433292047 1204 9 -0.14911448884125633 1204 10-11 -0.08992458867234632 1204 12-13 -0.0844789851443295 1204 14-15 -0.061676096623550336 1204 16-17 -0.026120873736495298 1204 18-19 0.062113648480163874 1204 20-21 -0.08240721062664846 1204 22-23 -0.00976398607190987 1204 24-25 -0.10344405365365361 1204 26-27 0.006556658959205208 1204 28-29 0.009896346213594143 1204 30-31 -0.0076833336142243525 1204 32-33 -0.1305919150579271 1204 34-35 -0.09812166248423182 1204 36-37 3.985781003805755E-4 1204 38-39 0.018371239736985956 1204 40-41 -0.11805271943340756 1204 42-43 -0.10978520934103386 1204 44-45 -0.061067719110674545 1204 46-47 -0.10056439618178104 1204 48-49 -0.10069880868400816 1204 50-51 -0.014281316548554912 1204 52-53 5.399223935143027E-5 1204 54-55 -0.09518690779968608 1204 56-57 -0.09862640588558946 1204 58-59 -0.14251430021310796 1204 60-61 -0.0365107282400885 1204 62-63 -0.039378971519077766 1204 64-65 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2401 26-27 -0.05803416029387165 2401 28-29 -0.051835428190969424 2401 30-31 0.053000287440283955 2401 32-33 -0.04813825549970119 2401 34-35 0.03151409659832893 2401 36-37 0.025435524813516963 2401 38-39 0.0301170253879377 2401 40-41 0.03884444255562158 2401 42-43 0.06875204393564616 2401 44-45 0.021275549874076205 2401 46-47 0.0269070320159841 2401 48-49 0.020738717898360903 2401 50-51 0.06077836582846885 2401 52-53 0.05761727042123965 2401 54-55 -0.07326881319456646 2401 56-57 0.005643246772486066 2401 58-59 0.0880207335217591 2401 60-61 0.03950275193751196 2401 62-63 0.09707365333716211 2401 64-65 0.07660046844626578 2401 66-67 -0.004954791582257201 2401 68-69 -0.009414052117755034 2401 70-71 -0.002771098186478582 2401 72-73 0.18624346383146673 2401 74-75 0.251520664310803 2401 76-77 0.21689669335121664 2401 78-79 0.0353406317241749 2401 80-81 0.23865643967373273 2401 82-83 0.021735712150679376 2401 84-85 0.12550229202427232 2401 86-87 0.017310704476436456 2401 88-89 0.1917743918855308 2401 90-91 0.38590680390830556 2402 1 0.07953584426901017 2402 2 -0.056666751287878014 2402 3 -5.808724820965949E-4 2402 4 0.11806376816290509 2402 5 0.1367452599627157 2402 6 -0.009111534635742657 2402 7 0.06484962731638433 2402 8 0.18521582471471731 2402 9 0.043839599963696685 2402 10-11 0.027352794020650606 2402 12-13 0.046886112426214765 2402 14-15 0.01051448985288772 2402 16-17 0.03172554162939889 2402 18-19 -0.009453965257776531 2402 20-21 0.01831952946444204 2402 22-23 0.03210894486737459 2402 24-25 0.012571585437129329 2402 26-27 0.03887593696381941 2402 28-29 -0.0031155163353417947 2402 30-31 0.044303030497282236 2402 32-33 0.06620847666151519 2402 34-35 0.04555434135200187 2402 36-37 0.03666138915649242 2402 38-39 -0.589562690176237 2402 40-41 0.06516121973160693 2402 42-43 0.04528738684587097 2402 44-45 0.1867755842976564 2402 46-47 0.12574069673827637 2402 48-49 0.1673479917137186 2402 50-51 0.12890342027087343 2402 52-53 0.0558995914778464 2402 54-55 0.0970751772288665 2402 56-57 0.21247776530453422 2402 58-59 0.13911208527429153 2402 60-61 0.08971955572275192 2402 62-63 0.07094588380501676 2402 64-65 0.09030681641340976 2402 66-67 0.16022472416160127 2402 68-69 0.0934524437105324 2402 70-71 0.18089423526351567 2402 72-73 0.14801672304130165 2402 74-75 0.11499056322186618 2402 76-77 0.06022578099565834 2402 78-79 0.05370407210620698 2402 80-81 0.10347368437446391 2402 82-83 0.3157616635021512 2402 84-85 0.09738714920373326 2402 86-87 0.26737959395522637 2402 88-89 0.06354053423991246 2402 90-91 0.28961596627627273 2403 1 -0.09469661496641635 2403 2 -0.018590331231614243 2403 3 0.0672769760515024 2403 4 0.16397225603937926 2403 5 0.18840454054908662 2403 6 0.11404876682924225 2403 7 0.028079179164784307 2403 8 0.005533661392952638 2403 9 0.018194376452434824 2403 10-11 0.11652018988632307 2403 12-13 -0.027155343713644697 2403 14-15 0.045331625559171584 2403 16-17 0.041714156715819684 2403 18-19 0.02251411934812353 2403 20-21 0.04929025800481668 2403 22-23 0.04214648685512401 2403 24-25 0.10162230946117035 2403 26-27 0.05964352477349166 2403 28-29 0.032345196303793955 2403 30-31 0.03287295362559206 2403 32-33 0.11150791887035183 2403 34-35 0.07115289448994844 2403 36-37 -0.01255031869089862 2403 38-39 0.11988803617686017 2403 40-41 0.12206883220679288 2403 42-43 0.08683587023863026 2403 44-45 0.058570207592907764 2403 46-47 0.12167865611357342 2403 48-49 0.047683822060378134 2403 50-51 -0.010232370801368518 2403 52-53 0.008184374571790443 2403 54-55 0.05909229860019849 2403 56-57 0.07783288951060285 2403 58-59 0.09694491962260798 2403 60-61 0.1244632100960743 2403 62-63 0.17496574800266984 2403 64-65 0.11266517224783001 2403 66-67 0.02136959760307633 2403 68-69 0.011739124915905563 2403 70-71 -0.11153568108200318 2403 72-73 -0.05425265760836595 2403 74-75 -0.01280496708106682 2403 76-77 0.32888851373079575 2403 78-79 0.09114691645518036 2403 80-81 -0.028244917871234065 2403 82-83 0.2817705404142288 2403 84-85 0.4187319765583055 2403 86-87 0.3293489318539571 2403 88-89 0.4141217115927347 2403 90-91 0.410388913136174 2404 1 -0.029675789623418325 2404 2 -2.8556125240442043E-4 2404 3 0.005884268622338595 2404 4 0.20841939196595405 2404 5 0.07045841056395119 2404 6 0.11483467961176075 2404 7 0.13770324639801146 2404 8 0.01030835069498437 2404 9 -0.036421178968545576 2404 10-11 0.10823311640213262 2404 12-13 0.0680854430065807 2404 14-15 0.06570076233357014 2404 16-17 0.06187874084818645 2404 18-19 0.07176016791591877 2404 20-21 0.08297953985010054 2404 22-23 0.07837652783294402 2404 24-25 0.14435766275185102 2404 26-27 0.07359616814538583 2404 28-29 0.08506056533963147 2404 30-31 0.06831708182088647 2404 32-33 0.001950050497477207 2404 34-35 0.1272983656579214 2404 36-37 0.10991924418364363 2404 38-39 0.10039128139921161 2404 40-41 0.11805702706664079 2404 42-43 0.12209407954427576 2404 44-45 0.01871847742506816 2404 46-47 0.11549471351661111 2404 48-49 0.0858675082372784 2404 50-51 0.07418154838056523 2404 52-53 0.04935622144927976 2404 54-55 0.052166747377505374 2404 56-57 0.012934804303917247 2404 58-59 -0.006237755398906586 2404 60-61 0.04406488851449808 2404 62-63 0.020759833048060727 2404 64-65 0.033044400253182005 2404 66-67 0.0037437388030241436 2404 68-69 -0.07902162344436192 2404 70-71 0.09300287388764161 2404 72-73 -0.03341278439966544 2404 74-75 0.03863367832921227 2404 76-77 0.0444801603655236 2404 78-79 0.14922111073263267 2404 80-81 0.3091327201639871 2404 82-83 -3.41882947736849E-4 2404 84-85 0.028292309464198695 2404 86-87 0.1378463706972468 2404 88-89 0.1343454355532785 2404 90-91 -0.42923889200886833 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 1.0 30 12.0 31 413.0 32 2759.0 33 4839.0 34 6165.0 35 7576.0 36 10758.0 37 15760.0 38 37567.0 39 260058.0 40 256414.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.892316734238495 11.911734919196045 26.73420529218591 31.46174305437955 2 30.74682484854926 16.381423371240302 25.086851684557338 27.784900095653096 3 28.577737489249937 18.56780924488895 19.692124810317406 33.16232845554371 4 27.959463542756204 19.973203701674517 19.38099554723221 32.686337208337065 5 28.45521166419291 20.361202147688445 19.39925820408353 31.784327984035116 6 28.509169513980893 23.06208307184529 22.1718615624201 26.256885851753715 7 26.978758869840387 22.192614581569327 22.12686901690458 28.70175753168571 8 26.521694376097837 23.14260478614429 22.05498055857166 28.280720279186216 9 26.467570502156658 23.082338018534937 22.7937880402841 27.65630343902431 10-11 26.52393570216595 21.700685015656077 22.46290190296884 29.312477379209128 12-13 27.067166731416087 21.570439067475537 22.32070221575835 29.041691985350027 14-15 27.500406759175323 21.300566806459003 21.98400523308131 29.215021201284365 16-17 27.666845972752114 21.032603823204198 21.692217783843194 29.60833242020049 18-19 27.76778865789395 21.341823808527664 21.75962359003988 29.130763943538508 20-21 27.722049003689058 21.343816098365988 21.739866715809818 29.194268182135136 22-23 27.66053705493075 20.84923014600164 21.86928254322439 29.62095025584322 24-25 27.767290585434367 21.215562440023774 21.85201603129223 29.165130943249622 26-27 27.950498238483735 21.374198518400455 21.720275865732948 28.955027377382862 28-29 27.86649001696767 21.026875989919013 21.813332403598075 29.29330158951524 30-31 27.78436343532248 21.337862494790965 21.96195039505644 28.915823674830115 32-33 27.897547219505487 21.327497613651424 21.902989268561097 28.871965898282 34-35 27.863451999641743 21.001505542357773 21.963943753278713 29.17109870472177 36-37 27.879779460423386 21.375655662907416 21.98943625180242 28.755128624866778 38-39 28.127126560370648 21.36044495010565 21.93360041294342 28.578828076580287 40-41 27.88642229304628 21.065697989474362 22.07637976799508 28.97149994948428 42-43 27.886975554842618 21.386522796433365 22.155737502633972 28.570764146090045 44-45 28.037990384274625 21.430427746211002 22.093625348973653 28.43795652054072 46-47 27.921526356828224 21.145812734189846 22.166744708603023 28.765916200378904 48-49 27.96802943879092 21.421946721443152 22.212643001111665 28.397380838654264 50-51 28.099397394026987 21.509391977091532 22.060750593952363 28.330460034929118 52-53 28.080161004310582 21.198150677386074 22.114638575410357 28.607049742892983 54-55 28.13468418513436 21.50210209081283 22.11662688044711 28.246586843605698 56-57 28.222262773236505 21.542711539767893 22.09214818406194 28.14287750293366 58-59 28.09398705323932 21.26927185494849 22.14803572520778 28.48870536660441 60-61 28.27103160427334 21.55065812975736 22.149554682323775 28.02875558364553 62-63 28.326282852328966 21.453300317518934 22.06130126710827 28.15911556304383 64-65 28.264781583154697 21.246979875395215 22.104444332919897 28.383794208530194 66-67 28.186318199893044 21.54091161070552 22.158733641134333 28.1140365482671 68-69 28.38080497358216 21.596021479183687 22.101743728591412 27.92142981864274 70-71 28.39753625724965 21.3829662254339 21.95262881543268 28.26686870188377 72-73 28.430348673833585 21.604373361469843 22.00434967512018 27.960928289576394 74-75 28.50917515537087 21.69859890412974 21.681437624878953 28.110788315620443 76-77 28.526580381037903 21.401122113844707 21.755008679235942 28.317288825881448 78-79 28.654871294932693 21.700026964800564 21.702101180228578 27.94300056003817 80-81 28.740179111037854 21.50930768039718 21.59321915379275 28.157294054772215 82-83 28.898518693271413 21.25544780659821 21.429280951612302 28.41675254851807 84-85 29.3740136770121 21.03993849391009 20.892647594383522 28.693400234694288 86-87 29.725429122795138 20.24015077134893 20.409429980476464 29.62499012537947 88-89 29.09804063992446 19.971308721785398 20.550581997130873 30.380068641159276 90-91 24.852379298367488 22.10837096213963 23.536818339701284 29.502431399791597 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.5 6 1.5 7 1.5 8 2.5 9 2.5 10 3.0 11 6.5 12 11.5 13 13.5 14 18.5 15 37.5 16 50.5 17 60.5 18 91.5 19 133.5 20 181.5 21 239.0 22 299.0 23 386.5 24 532.0 25 717.5 26 888.0 27 1087.5 28 1420.5 29 1785.0 30 2109.5 31 2512.5 32 2937.0 33 3375.0 34 3998.0 35 4713.0 36 5476.0 37 6316.5 38 7267.0 39 8545.5 40 9880.5 41 11185.0 42 12645.5 43 14265.5 44 16004.5 45 17693.0 46 19441.0 47 21239.0 48 23224.0 49 25504.0 50 27592.5 51 29377.0 52 31167.5 53 33169.5 54 35067.5 55 36578.0 56 37839.5 57 38849.5 58 39937.5 59 40813.5 60 41224.5 61 41205.5 62 40831.0 63 40089.0 64 38775.0 65 36806.5 66 33843.5 67 30840.0 68 27762.5 69 24390.0 70 21208.5 71 18133.5 72 15119.0 73 12233.0 74 9829.0 75 7922.0 76 6315.5 77 4864.0 78 3685.0 79 2743.5 80 2014.5 81 1538.0 82 1161.5 83 816.0 84 592.0 85 455.0 86 326.5 87 239.0 88 181.0 89 118.0 90 80.5 91 59.0 92 43.0 93 34.5 94 26.5 95 18.5 96 10.0 97 5.5 98 4.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.02423952636629577 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0726409537917864 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.297174321606383E-4 64-65 1.792355603351705E-4 66-67 0.0 68-69 4.3113390372348796E-4 70-71 0.0 72-73 0.0 74-75 3.064504760708146E-4 76-77 3.535317825072474E-4 78-79 4.148413646621532E-4 80-81 9.989361330183355E-4 82-83 6.208288064566196E-4 84-85 8.092841072787012E-4 86-87 0.0011285153252381167 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 3988.0 32-33 9405.0 34-35 11514.0 36-37 13397.0 38-39 15502.0 40-41 17169.0 42-43 18578.0 44-45 19665.0 46-47 20876.0 48-49 21667.0 50-51 22708.0 52-53 23031.0 54-55 23454.0 56-57 23835.0 58-59 23989.0 60-61 24128.0 62-63 24479.0 64-65 23766.0 66-67 23377.0 68-69 23470.0 70-71 22882.0 72-73 22770.0 74-75 21895.0 76-77 21142.0 78-79 20500.0 80-81 19794.0 82-83 19061.0 84-85 17738.0 86-87 16972.0 88-89 16176.0 90-91 15394.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.75543634038337 #Duplication Level Percentage of deduplicated Percentage of total 1 99.77858957091958 99.53456740075109 2 0.20544283080236725 0.40988078459387406 3 0.01188541268892671 0.03556903586608239 4 0.002256089588124742 0.009002288051455178 5 0.0013946285959073887 0.006956089205875889 6 2.507844733302549E-4 0.0015010268738671689 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 1.806829317741172E-4 0.0025233746577741495 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002324338144713293 0.0 0.0 0.0 0.0 2 0.002324338144713293 0.0 0.0 0.0 0.0 3 0.002324338144713293 0.0 0.0 0.0 0.0 4 0.002324338144713293 0.0 0.0 0.0 0.0 5 0.002324338144713293 0.0 0.0 1.6602415319380665E-4 0.0 6 0.002324338144713293 0.0 0.0 1.6602415319380665E-4 0.0 7 0.0026563864511009064 0.0 0.0 1.6602415319380665E-4 0.0 8 0.007305062740527492 0.0 0.0 1.6602415319380665E-4 0.0 9 0.007803135200108912 0.0 0.0 1.6602415319380665E-4 0.0 10-11 0.007803135200108912 0.0 0.0 1.6602415319380665E-4 0.0 12-13 0.007803135200108912 0.0 0.0 1.6602415319380665E-4 1.6602415319380665E-4 14-15 0.007803135200108912 0.0 0.0 1.6602415319380665E-4 2.4903622979070996E-4 16-17 0.007886147276705816 0.0 0.0 1.6602415319380665E-4 3.320483063876133E-4 18-19 0.007969159353302719 0.0 0.0 1.6602415319380665E-4 4.980724595814199E-4 20-21 0.007969159353302719 0.0 1.6602415319380665E-4 1.6602415319380665E-4 6.640966127752266E-4 22-23 0.007969159353302719 0.0 1.6602415319380665E-4 1.6602415319380665E-4 6.640966127752266E-4 24-25 0.007969159353302719 0.0 3.320483063876133E-4 1.6602415319380665E-4 6.640966127752266E-4 26-27 0.008052171429899623 0.0 3.320483063876133E-4 1.6602415319380665E-4 6.640966127752266E-4 28-29 0.008301207659690332 0.0 3.320483063876133E-4 1.6602415319380665E-4 9.131328425659365E-4 30-31 0.008467231812884139 0.0 3.320483063876133E-4 1.6602415319380665E-4 9.961449191628398E-4 32-33 0.008716268042674849 0.0 3.320483063876133E-4 1.6602415319380665E-4 9.961449191628398E-4 34-35 0.008965304272465557 0.0 3.320483063876133E-4 1.6602415319380665E-4 9.961449191628398E-4 36-37 0.009131328425659365 0.0 3.320483063876133E-4 1.6602415319380665E-4 9.961449191628398E-4 38-39 0.009463376732046978 0.0 3.320483063876133E-4 1.6602415319380665E-4 9.961449191628398E-4 40-41 0.009463376732046978 0.0 3.320483063876133E-4 1.6602415319380665E-4 9.961449191628398E-4 42-43 0.009961449191628398 0.0 3.320483063876133E-4 1.6602415319380665E-4 9.961449191628398E-4 44-45 0.01029349749801601 0.0 3.320483063876133E-4 1.6602415319380665E-4 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