##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-34B_S42_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1074289
Sequences flagged as poor quality	0
Sequence length	61
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.46206653889224	40.0	40.0	40.0	40.0	40.0
2	39.21732606402932	40.0	40.0	40.0	40.0	40.0
3	39.14092390408912	40.0	40.0	40.0	40.0	40.0
4	39.01770380223571	40.0	40.0	40.0	40.0	40.0
5	39.0650299872753	40.0	40.0	40.0	40.0	40.0
6	39.19030726368789	40.0	40.0	40.0	40.0	40.0
7	39.21097209410131	40.0	40.0	40.0	40.0	40.0
8	39.199288087283776	40.0	40.0	40.0	40.0	40.0
9	39.22999490826025	40.0	40.0	40.0	40.0	40.0
10	39.23467893648729	40.0	40.0	40.0	40.0	40.0
11	39.233993832199715	40.0	40.0	40.0	40.0	40.0
12	39.23722573720852	40.0	40.0	40.0	40.0	40.0
13	39.0672379592456	40.0	40.0	40.0	40.0	40.0
14	39.231752349693615	40.0	40.0	40.0	40.0	40.0
15	39.143076025166415	40.0	40.0	40.0	40.0	40.0
16	39.325418020662966	40.0	40.0	40.0	40.0	40.0
17	39.278521887499544	40.0	40.0	40.0	40.0	40.0
18	39.355726438602645	40.0	40.0	40.0	40.0	40.0
19	39.31497018027738	40.0	40.0	40.0	40.0	40.0
20	39.316258474209455	40.0	40.0	40.0	40.0	40.0
21	39.29556758004597	40.0	40.0	40.0	40.0	40.0
22	39.358362600752685	40.0	40.0	40.0	40.0	40.0
23	39.35682483949849	40.0	40.0	40.0	40.0	40.0
24	39.35384612520467	40.0	40.0	40.0	40.0	40.0
25	39.305281912036705	40.0	40.0	40.0	40.0	40.0
26	39.27606072481427	40.0	40.0	40.0	40.0	40.0
27	39.297693637373186	40.0	40.0	40.0	40.0	40.0
28	39.30572871918078	40.0	40.0	40.0	40.0	40.0
29	39.31518986045654	40.0	40.0	40.0	40.0	40.0
30	39.2981925720174	40.0	40.0	40.0	40.0	40.0
31	39.26210172495483	40.0	40.0	40.0	40.0	40.0
32	39.28927504610026	40.0	40.0	40.0	40.0	40.0
33	39.28052879625501	40.0	40.0	40.0	40.0	40.0
34	39.28121390054259	40.0	40.0	40.0	40.0	40.0
35	39.28981121467314	40.0	40.0	40.0	40.0	40.0
36	39.240074132751985	40.0	40.0	40.0	40.0	40.0
37	39.261014494237585	40.0	40.0	40.0	40.0	40.0
38	39.25213792564198	40.0	40.0	40.0	40.0	40.0
39	38.01238958976588	40.0	40.0	40.0	40.0	40.0
40	39.2387634984627	40.0	40.0	40.0	40.0	40.0
41	39.2490288925978	40.0	40.0	40.0	40.0	40.0
42	39.219334834481224	40.0	40.0	40.0	40.0	40.0
43	39.24479167151483	40.0	40.0	40.0	40.0	40.0
44	39.19327853119598	40.0	40.0	40.0	40.0	40.0
45	39.058350220471404	40.0	40.0	40.0	40.0	40.0
46	39.050672584379065	40.0	40.0	40.0	40.0	40.0
47	39.15260046412092	40.0	40.0	40.0	40.0	40.0
48	39.231245968263664	40.0	40.0	40.0	40.0	40.0
49	39.21661303429524	40.0	40.0	40.0	40.0	40.0
50	39.20211786586291	40.0	40.0	40.0	40.0	40.0
51	39.163212133792676	40.0	40.0	40.0	40.0	40.0
52	39.17303816756944	40.0	40.0	40.0	40.0	40.0
53	39.101738917553845	40.0	40.0	40.0	40.0	40.0
54	39.025789149847014	40.0	40.0	40.0	40.0	40.0
55	39.07257730461728	40.0	40.0	40.0	40.0	40.0
56	39.07416346997875	40.0	40.0	40.0	40.0	40.0
57	39.01349450659925	40.0	40.0	40.0	40.0	40.0
58	39.00072699245734	40.0	40.0	40.0	40.0	40.0
59	38.96953985380098	40.0	40.0	40.0	40.0	40.0
60	38.94956385106801	40.0	40.0	40.0	40.0	40.0
61	38.84341364381466	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.027142505030759878
1101	2	0.02252841683005613
1101	3	0.05634146192203815
1101	4	0.036856610251639665
1101	5	0.10080621564774361
1101	6	0.11343209043924674
1101	7	-0.017824341999080673
1101	8	0.04624921092737111
1101	9	0.028261197692991402
1101	10	0.03520841935468866
1101	11	-0.0762776487030905
1101	12	0.010489943255961975
1101	13	0.0014495764973929681
1101	14	0.021073252136510234
1101	15	0.012350884151359764
1101	16	0.14615783001917748
1101	17	0.026261838887982947
1101	18	-0.042545818537902846
1101	19	-0.007850128205824092
1101	20	0.02223371675649588
1101	21	0.013952867147715153
1101	22	-0.011577838757958148
1101	23	0.041708826071989336
1101	24	0.03642838288247674
1101	25	0.1232366133279541
1101	26	0.03588900307958909
1101	27	0.0894257805900196
1101	28	0.06384489296091544
1101	29	-0.11007359822381346
1101	30	0.04035109429017325
1101	31	0.0683965593573177
1101	32	0.09519554165923694
1101	33	0.11062039751711694
1101	34	0.0425020474900748
1101	35	0.00580823145197229
1101	36	0.031417550063501665
1101	37	0.022789519251467993
1101	38	-0.025085881924702846
1101	39	1.1916339392529665
1101	40	-0.129418840381895
1101	41	0.02089729012436692
1101	42	0.03940527267112515
1101	43	-0.07116933321189123
1101	44	-0.06144629330169238
1101	45	0.08879817689210512
1101	46	-0.02854176544224174
1101	47	-0.011103559478911507
1101	48	0.08263871191271477
1101	49	0.007805540329805183
1101	50	0.03138968692991995
1101	51	-0.032393750083095085
1101	52	0.040786651397795026
1101	53	-0.010315667458755229
1101	54	0.06892427016933311
1101	55	-0.14076692297359727
1101	56	-0.046215797827557026
1101	57	0.011805786397950158
1101	58	0.023066723161932146
1101	59	0.047983170215090354
1101	60	0.05545279812671566
1101	61	0.09967884668552074
1102	1	0.05266161679510617
1102	2	-0.009080193973382222
1102	3	0.10949489092030973
1102	4	0.02877173792933263
1102	5	-0.030722457461891395
1102	6	-0.12038258151823555
1102	7	-0.11258681334103926
1102	8	-0.15413342142705488
1102	9	-0.10156563136980168
1102	10	-0.11434253904140235
1102	11	-0.05278815944809878
1102	12	-0.04983732864933188
1102	13	-0.014575293920657373
1102	14	-0.025148818475805967
1102	15	0.04631001934469481
1102	16	0.014791155749179552
1102	17	-0.07366267581760866
1102	18	-0.05878611658836519
1102	19	-0.06895554252898961
1102	20	-0.05499006837207787
1102	21	-0.025935151582501703
1102	22	0.019397846346436154
1102	23	0.016621267925366112
1102	24	-0.013343277526850272
1102	25	-0.1084789235594954
1102	26	-0.0686379226345224
1102	27	0.06433822244339638
1102	28	-0.02369955397230683
1102	29	0.06785455648952876
1102	30	-0.08398935123746298
1102	31	0.10968297399077898
1102	32	-0.0275125857506211
1102	33	-0.005074858348578459
1102	34	0.0392411786693998
1102	35	-0.02606033097786309
1102	36	0.041785133307961075
1102	37	-0.018472578431435238
1102	38	-0.13851623646583988
1102	39	1.2377347676047563
1102	40	-0.018139337242267572
1102	41	-0.02297391360578871
1102	42	-0.009147800272195639
1102	43	0.08993525912984524
1102	44	-0.044181600897417184
1102	45	0.04178152423337167
1102	46	0.06437124768822855
1102	47	-0.0195196218179845
1102	48	-0.08765289284847455
1102	49	0.037778065600598154
1102	50	-0.007918834894027782
1102	51	-0.057206286943824125
1102	52	0.042429586775668326
1102	53	0.020815851624576
1102	54	-0.08340121803626488
1102	55	-0.013365624105944107
1102	56	-0.03789426335262647
1102	57	-0.002076794925123693
1102	58	0.012452202997010886
1102	59	-0.016631762310510112
1102	60	0.12463863237279327
1102	61	0.043752257846797704
1103	1	0.03334386093863628
1103	2	-0.03863959995153721
1103	3	-0.05623235822358197
1103	4	-0.06345358144236712
1103	5	-0.004734023051767622
1103	6	-0.0019799127012163353
1103	7	-0.012130731866825784
1103	8	0.08086733431834148
1103	9	0.03269294233361819
1103	10	-0.044042616815403335
1103	11	-0.050618364558168594
1103	12	-0.01068383509797144
1103	13	-0.07231782233527895
1103	14	0.04399578941936255
1103	15	-0.10814814104740833
1103	16	0.0648638701351345
1103	17	0.007221262406154949
1103	18	-0.11508279708009894
1103	19	0.020671683605804958
1103	20	0.008807630223358842
1103	21	0.008791796712493749
1103	22	0.05271441091288409
1103	23	-0.03807474979513614
1103	24	0.01698713367621707
1103	25	-0.04718724769643501
1103	26	-0.02613817774654592
1103	27	0.010802634081755969
1103	28	0.0166344880481617
1103	29	0.01747016639566823
1103	30	0.0037671386461610723
1103	31	-0.06328681384041346
1103	32	-0.06810851720617705
1103	33	0.03785011586955278
1103	34	-0.06371274149233841
1103	35	-0.04334264668062815
1103	36	0.05309343727464011
1103	37	-0.08554832798977685
1103	38	0.017670462398221787
1103	39	1.2032260605056635
1103	40	0.07513422187351182
1103	41	0.015969522794506474
1103	42	0.06115723698183473
1103	43	-0.01654496135513739
1103	44	-0.011240479388305857
1103	45	-0.06541923319998233
1103	46	0.06150410397700057
1103	47	-0.032935658334508844
1103	48	-0.10822655858702035
1103	49	-0.013261730084721535
1103	50	-0.0013173797197083559
1103	51	0.0896369607204619
1103	52	0.02558238945724156
1103	53	-0.014528310052597249
1103	54	0.02878396371048808
1103	55	0.09240735850355719
1103	56	0.04961193115943985
1103	57	0.02749795613711825
1103	58	0.05629720025663687
1103	59	0.03244011159109306
1103	60	-0.0760317602507925
1103	61	0.11815340954650821
1104	1	0.04030879911725549
1104	2	0.01124755850063508
1104	3	-0.042064929592811495
1104	4	-0.0472838300970011
1104	5	0.040816287963714615
1104	6	0.14970666204385452
1104	7	-0.0081048282086158
1104	8	-0.023164364738022414
1104	9	0.13912547154069443
1104	10	0.04481880509185743
1104	11	0.002177965664124315
1104	12	-0.005462409131403945
1104	13	0.049837023111074075
1104	14	0.08537678808878013
1104	15	0.04081805524720039
1104	16	0.0378794165648344
1104	17	0.10327201490949989
1104	18	-0.041193579616603415
1104	19	0.01564900520897794
1104	20	0.021226485158976516
1104	21	0.006033288422663929
1104	22	0.028110204128687144
1104	23	-0.016391356192187345
1104	24	0.028342739728500987
1104	25	-0.017505023633518135
1104	26	0.11752797087732603
1104	27	-0.038462130665145366
1104	28	0.046492176858563994
1104	29	0.11831650542072225
1104	30	0.07077206422844284
1104	31	0.047665957207698284
1104	32	0.10224480721388574
1104	33	2.0314199954185597E-4
1104	34	0.0322111365012816
1104	35	0.04678988234929449
1104	36	-0.1067036579908276
1104	37	0.03469057738689685
1104	38	-0.044477801432933006
1104	39	1.2583918396834477
1104	40	0.07939497834759379
1104	41	0.014249968269133717
1104	42	0.047873880732637986
1104	43	0.03750627924389249
1104	44	-0.024780284114598317
1104	45	0.0032478834325289085
1104	46	-0.023099935778247982
1104	47	0.04645606612374564
1104	48	-0.013695263310466999
1104	49	4.490898673736865E-5
1104	50	0.04687502547428579
1104	51	0.0798186958019258
1104	52	0.12028914948127323
1104	53	0.045017339168232695
1104	54	0.10334230923988486
1104	55	0.03932927238866313
1104	56	-0.01635775241266657
1104	57	0.08815814393669541
1104	58	0.14367965680190764
1104	59	0.03598281232930134
1104	60	0.1400395789914839
1104	61	0.08435991147708677
1201	1	0.06849666887944039
1201	2	0.21121161836372693
1201	3	0.029946128283718565
1201	4	0.07435960569856093
1201	5	0.04984004785914209
1201	6	0.0896509650226065
1201	7	0.059804026517142006
1201	8	0.0012603213123085766
1201	9	0.02300010364685079
1201	10	-0.005028602190741083
1201	11	0.052624601694482465
1201	12	0.05433511677403402
1201	13	0.1301530381447833
1201	14	0.09646048172934485
1201	15	0.09215775951898308
1201	16	0.06901145614382642
1201	17	0.1005816001236326
1201	18	-0.006714827099429499
1201	19	0.00222546598191542
1201	20	0.13053273860692371
1201	21	0.01703545434244802
1201	22	0.054681447127499894
1201	23	0.04030913541996739
1201	24	0.0747483183986759
1201	25	0.17797976737016796
1201	26	0.06701169658546036
1201	27	0.05531580956416349
1201	28	0.116139949928872
1201	29	0.06217821538313473
1201	30	0.14044531471149213
1201	31	0.11121783465522839
1201	32	0.09845240940208555
1201	33	0.14424020450140773
1201	34	0.02694478543748602
1201	35	0.021684100131579953
1201	36	0.12320621311384627
1201	37	0.09941577461604112
1201	38	0.056085283358612514
1201	39	1.311266642997822
1201	40	0.12671910267193454
1201	41	0.08927517392280748
1201	42	0.16853626628809337
1201	43	0.07060789267748646
1201	44	0.12927653527160743
1201	45	0.16980867729512283
1201	46	0.14795541339138651
1201	47	0.1820047340966653
1201	48	0.11027307132277997
1201	49	0.12168698598695471
1201	50	0.13127150293323098
1201	51	0.09180564864901442
1201	52	0.08103728861104997
1201	53	0.08221096478750667
1201	54	0.10301517926023962
1201	55	0.11963000097473042
1201	56	0.0637034884935943
1201	57	0.15213430523114368
1201	58	0.09790387977479043
1201	59	0.1275122859591562
1201	60	0.1617966100780066
1201	61	0.2561147005512794
1202	1	0.06455545623241932
1202	2	0.04343895947972953
1202	3	0.0521341533669073
1202	4	0.05725493499779333
1202	5	-0.010468330409416637
1202	6	0.03852227639900008
1202	7	0.02397893893463987
1202	8	-0.03399369540249353
1202	9	-0.03785189015922441
1202	10	-0.016554349799385193
1202	11	0.005028041298253072
1202	12	-0.021401784287938597
1202	13	0.0056746894181642915
1202	14	-0.11540131912067153
1202	15	-0.021607519372267348
1202	16	-0.07432458611541648
1202	17	-0.025251669147266398
1202	18	0.0025184004393636883
1202	19	-0.07696119731689777
1202	20	0.04040512623225112
1202	21	0.040081458493695266
1202	22	0.0313279432782565
1202	23	-0.11715541974877652
1202	24	-0.09775901217447824
1202	25	-0.06359007349873735
1202	26	-0.06370724869056943
1202	27	0.05042052419346277
1202	28	-0.08342186300149024
1202	29	-0.11175816512577796
1202	30	-0.050856562134875105
1202	31	0.04993891046488841
1202	32	-0.014372524430562805
1202	33	0.00806451235254002
1202	34	-0.03459496438954801
1202	35	0.0011832050878126665
1202	36	-0.2670562049319898
1202	37	-0.20156740075762514
1202	38	-0.2299168911035352
1202	39	1.1028202852219238
1202	40	-0.19182862018298152
1202	41	-0.2918405008742937
1202	42	-0.21220876155160795
1202	43	-0.11786196686104233
1202	44	-0.20256780398841556
1202	45	-0.12364899207680935
1202	46	-0.10223677391985575
1202	47	-0.22490796893549714
1202	48	0.024228066545553872
1202	49	-0.13592088600531582
1202	50	-0.007686786929163247
1202	51	-0.06987741800154623
1202	52	0.02385469968323406
1202	53	-0.0788118011722787
1202	54	0.08964855176317599
1202	55	-0.17055636580651878
1202	56	-0.13984895667810804
1202	57	-0.09110344694606454
1202	58	-0.09771809327505565
1202	59	-0.11151244580226916
1202	60	-0.06310647260647073
1202	61	0.004122816346885827
1203	1	-0.028109819654460466
1203	2	0.040714342504969636
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2403	4	0.11000689321118529
2403	5	0.04118040397833056
2403	6	0.008223644631350169
2403	7	0.10207195909613631
2403	8	0.03128506517303009
2403	9	-0.005942715257511111
2403	10	0.060316977160795204
2403	11	0.08507598404018779
2403	12	0.06537329786683443
2403	13	0.15948031354720626
2403	14	8.733491028181106E-4
2403	15	0.05331279841027481
2403	16	0.025387138403793585
2403	17	-0.027373444992534246
2403	18	0.018701744072060933
2403	19	0.18448826304264543
2403	20	0.0477879384960076
2403	21	0.03944357383733177
2403	22	0.026359700541981113
2403	23	-0.03739493423067586
2403	24	0.05057318449322423
2403	25	-0.05766589318763238
2403	26	-0.037878434448060716
2403	27	0.07087303451684335
2403	28	0.0021781310216724137
2403	29	0.018254325744436528
2403	30	-0.0726061876044426
2403	31	-0.008730824309573393
2403	32	0.024295808634164473
2403	33	-0.039177730482649054
2403	34	0.11487638825678204
2403	35	-0.027785937019579876
2403	36	0.02531269518480883
2403	37	-0.0010709678242832865
2403	38	-0.04624168645925408
2403	39	1.1847192674881626
2403	40	-0.0751081798042037
2403	41	-0.02352301860111794
2403	42	-0.09211745830665308
2403	43	0.021565815381570985
2403	44	-0.043375203023515496
2403	45	0.08675510945393938
2403	46	-0.015141148499388635
2403	47	-0.021277611887299486
2403	48	-0.02113111801514833
2403	49	0.02055830376377088
2403	50	0.019029658221114687
2403	51	0.0025130627528682226
2403	52	-0.0971733298864379
2403	53	-0.003025198315420141
2403	54	0.0140430327267822
2403	55	-0.04856741796755415
2403	56	0.03276199095949295
2403	57	-0.05031404956385188
2403	58	-0.03781456932699001
2403	59	-0.011368468155623646
2403	60	-0.037506770309690296
2403	61	-0.0017028173664428436
2404	1	0.06139380931200833
2404	2	-0.14128924698760414
2404	3	-0.056850006108135176
2404	4	0.03933799982980446
2404	5	-0.06775282387904014
2404	6	-0.02784001857479268
2404	7	-0.0026226977651759853
2404	8	0.02730041778502823
2404	9	0.02971076365869152
2404	10	0.009939184408032986
2404	11	0.043881761778898465
2404	12	0.05419414825048818
2404	13	-0.027479081463518185
2404	14	0.046856310177631144
2404	15	0.07842519216114852
2404	16	-0.009029276780836426
2404	17	0.1463168137816666
2404	18	-0.04248733460844534
2404	19	0.0011814614709209081
2404	20	0.023896331322333708
2404	21	-0.016266614684674607
2404	22	0.04999738410759136
2404	23	-0.033058700308437494
2404	24	0.0046716091902254675
2404	25	-0.06310571817567023
2404	26	0.08574655293714528
2404	27	-0.03432621412971315
2404	28	-0.06634868884557221
2404	29	-0.006490204317842085
2404	30	0.029773857906484125
2404	31	-0.06436613792150325
2404	32	-0.0048370422443113625
2404	33	-0.19144074325370042
2404	34	-0.0014987545752447318
2404	35	0.010816962267156782
2404	36	0.04694503333292488
2404	37	0.014685317598875258
2404	38	0.01203052615944955
2404	39	1.3496491617843205
2404	40	0.0410325720050011
2404	41	-0.00827783582495556
2404	42	0.05927309899055899
2404	43	0.05522697060376913
2404	44	0.09711617171238629
2404	45	-0.05860638986730038
2404	46	-0.0012339478118406078
2404	47	0.12528514142591263
2404	48	0.03589752001023783
2404	49	-4.782905346729649E-4
2404	50	-0.044789808604413395
2404	51	0.030096328536011185
2404	52	-0.037143184596558854
2404	53	0.0029810716444345076
2404	54	0.019831188818187684
2404	55	0.12798761997449049
2404	56	-0.014012039817352218
2404	57	-0.05118976046856716
2404	58	0.04533239058888938
2404	59	0.16722772494351545
2404	60	-0.037357020489551473
2404	61	0.055527657373040995
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	10.0
17	28.0
18	72.0
19	150.0
20	191.0
21	263.0
22	358.0
23	499.0
24	820.0
25	1062.0
26	1456.0
27	1822.0
28	2553.0
29	3016.0
30	3904.0
31	4833.0
32	6546.0
33	7949.0
34	10068.0
35	12864.0
36	18791.0
37	26923.0
38	67571.0
39	464636.0
40	437903.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.44719716947674	12.903976490497435	28.207865853601778	29.440960486424046
2	30.964220265498593	16.60520865924701	26.071324811156305	26.359246264098097
3	27.86136293187947	19.577915398771463	20.714017762478743	31.84670390687032
4	27.288957895834265	20.49338724811224	20.673878885364076	31.543775970689424
5	27.453506458690352	20.612795998097347	20.099153952055733	31.83454359115657
6	28.786574189999154	23.039889638635415	22.55193900337805	25.621597167987385
7	27.42567409700742	22.32937319473624	22.260862765978242	27.9840899422781
8	25.750798900482085	24.460457102325353	22.488362070169202	27.30038192702336
9	25.62494822156794	24.318409664438526	23.538545028386217	26.518097085607316
10	26.560823018759383	22.11983926112992	23.338878085878196	27.980459634232503
11	25.895173458910964	22.954065433044555	22.838081745228706	28.31267936281578
12	27.301871284170275	22.964025508964532	22.58414635168004	27.14995685518515
13	26.009109280649806	21.658976308982034	22.812390334444455	29.5195240759237
14	26.429852674652725	22.55836185607411	22.64232436523133	28.36946110404184
15	26.690117836075768	22.290836078559867	22.456154721867208	28.56289136349716
16	26.596939929572024	22.762403785201187	22.146461520130988	28.4941947650958
17	26.862138586544216	21.75364357263269	22.089772863726616	29.29444497709648
18	27.654942012810334	22.610768610681113	22.356740132310765	27.37754924419779
19	26.630450465377564	22.096940394996132	23.054317785996133	28.21829135363017
20	26.74764425587528	22.17997205593653	22.10755206466789	28.964831623520297
21	26.678296063722147	22.711672557384468	23.015501415354713	27.594529963538676
22	27.544264159830362	21.631050862477416	22.469465851367744	28.35521912632448
23	26.761886233592634	22.99641902690989	22.046116082357727	28.195578657139748
24	27.043374734359187	23.558279010582815	22.153442881757144	27.244903373300854
25	26.885875216073142	21.78026583163376	22.11453342629404	29.219325525999057
26	27.062829462090743	22.08511862264251	22.881366187310864	27.970685727955885
27	27.03360082808257	23.109517085253593	21.889733581931864	27.967148504731966
28	26.891739559839113	22.29595574375238	22.17094282823337	28.641361868175135
29	26.94433248408948	23.603052809811885	21.940278640105223	27.512336065993416
30	27.97357135742803	23.317189322426273	22.02051775639516	26.688721563750534
31	26.802936639954428	22.47933284246604	23.129809576380286	27.58792094119925
32	26.98994404671369	22.71232415113624	22.132684966522042	28.165046835628026
33	27.13189839977883	24.308263418875182	22.103921756622285	26.455916424723704
34	27.3147169895624	22.835940794330018	22.075530886009258	27.773811330098326
35	27.40733638713605	24.149926137193994	21.6944416260429	26.748295849627056
36	27.58801402602093	23.891522672204594	21.562354264076053	26.958109037698424
37	28.419913077393517	23.61822563574606	21.43566582176677	26.526195465093654
38	27.593785284965218	24.112412954056126	22.01660819388451	26.27719356709414
39	28.11771861335014	24.53223771634576	21.083196493308208	26.26684717699589
40	27.671324941426377	24.34940691005865	20.90666478014761	27.07260336836736
41	27.66285422265331	25.5110123998291	20.81795494508461	26.008178432432988
42	27.91986141531748	25.92793931614305	20.646213449081205	25.505985819458267
43	27.965659147585054	25.807394472064782	20.401865792165797	25.825080588184374
44	28.027095129895212	25.316092783226864	21.00682404827751	25.649988038600412
45	28.06553916124991	25.811117864932058	20.107438501185435	26.015904472632595
46	28.064980652319814	25.858218784703187	20.57500356049443	25.501797002482572
47	29.29146626280265	25.956423271577762	19.41544593680099	25.3366645288186
48	29.371891548735956	26.411421879959672	19.288385155204978	24.92830141609939
49	29.01304956115161	26.4009032951096	19.20051308353711	25.385534060201675
50	29.081001480979513	26.546022532111934	18.903944841658063	25.469031145250487
51	29.10743757033722	26.991061064573874	18.73853311352904	25.16296825155987
52	28.94183967256483	26.89536986788471	18.681192863372893	25.481597596177565
53	28.991733136986415	26.927577216186705	18.43954466628626	25.64114498054062
54	28.651508113738483	27.54230937857504	18.49502322000877	25.31115928767771
55	28.743103578273633	27.056406609394678	18.35148642497503	25.84900338735666
56	28.531894117877034	27.218932708051558	18.271898902436867	25.977274271634542
57	28.346748407551413	27.576936932240763	18.281300469426757	25.795014190781067
58	28.031283946870904	27.422881552356955	18.123987120784072	26.421847379988066
59	27.948159201108822	27.668253142310867	17.95233870960235	26.431248946977952
60	27.7111652451063	27.869502526787482	17.932046218475662	26.487286009630555
61	27.678120133409166	27.705114731696963	17.869307048661952	26.74745808623192
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18.0
1	15.0
2	11.5
3	11.0
4	12.0
5	13.0
6	13.0
7	13.5
8	14.0
9	26.0
10	38.0
11	31.0
12	29.5
13	35.0
14	47.5
15	60.0
16	64.5
17	89.0
18	109.0
19	131.0
20	224.0
21	295.0
22	407.0
23	519.0
24	731.0
25	1165.5
26	1388.0
27	1937.0
28	2486.0
29	3285.0
30	4736.5
31	5389.0
32	6244.5
33	7100.0
34	8126.0
35	11181.5
36	13211.0
37	14689.0
38	16167.0
39	18322.5
40	23030.5
41	25583.0
42	28369.5
43	33530.0
44	35904.0
45	38524.0
46	41144.0
47	43769.0
48	49110.0
49	51826.0
50	54476.5
51	57127.0
52	59442.0
53	64978.5
54	68200.0
55	70648.0
56	73096.0
57	74158.0
58	75363.5
59	75507.0
60	73785.0
61	72051.0
62	72039.0
63	62978.5
64	53918.0
65	48735.0
66	38553.0
67	33554.0
68	29174.5
69	24795.0
70	21170.0
71	14822.0
72	12099.0
73	9994.0
74	7889.0
75	6429.5
76	3998.0
77	3026.0
78	2384.5
79	1743.0
80	1403.5
81	845.0
82	626.0
83	459.5
84	227.0
85	161.0
86	137.5
87	114.0
88	90.0
89	51.0
90	36.0
91	26.5
92	17.0
93	13.5
94	6.5
95	3.0
96	2.5
97	2.0
98	4.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03583765634759362
3	1.861696433641227E-4
4	9.308482168206135E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.1216925799296096
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1074289.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.09051269037344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.18538616163131	59.24786624048325
2	12.768437429651037	18.409664011169944
3	2.6600271000400757	5.752881522365291
4	0.7545158578385668	2.1757374009839685
5	0.3132593177914403	1.1291512412310778
6	0.2062048752715658	0.8919249106549012
7	0.15248523508059225	0.769491714227041
8	0.11283790650920016	0.6507634024925333
9	0.10236338816420097	0.6641486220132858
>10	0.7377840897105594	9.136211714358579
>50	0.006000863487339385	0.2611728894663422
>100	5.582198592873847E-4	0.046681132785971795
>500	0.0	0.0
>1k	0.0	0.0
>5k	1.3955496482184618E-4	0.8643051977678528
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTCCAAATCTGGGGGGGGGGGGGGG	8591	0.799691703070589	TruSeq Adapter, Index 16 (97% over 39bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.8890531318853679	0.0	0.0	0.0	0.0
2	0.8901701497455526	0.0	0.0	0.0	0.0
3	0.8917525917141477	0.0	0.0	0.0	0.0
4	0.8930557792176965	0.0	0.0	0.0	0.0
5	0.893800457791153	0.0	0.0	0.0	0.0
6	0.8974307658367534	0.0	1.861696433641227E-4	0.0	0.0
7	0.8999440560221691	0.0	1.861696433641227E-4	0.0	2.79254465046184E-4
8	0.9295450293170646	0.0	1.861696433641227E-4	0.0	2.79254465046184E-4
9	0.9328029980759367	0.0	1.861696433641227E-4	0.0	2.79254465046184E-4
10	0.9353162882613524	0.0	1.861696433641227E-4	0.0	2.79254465046184E-4
11	0.9396912748804093	0.0	1.861696433641227E-4	0.0	2.79254465046184E-4
12	0.9490928418702975	0.0	1.861696433641227E-4	0.0	2.79254465046184E-4
13	0.9655688553080223	0.0	1.861696433641227E-4	0.0	2.79254465046184E-4
14	0.9829757169625678	0.0	1.861696433641227E-4	0.0	2.79254465046184E-4
15	1.0070846857782216	0.0	1.861696433641227E-4	0.0	2.79254465046184E-4
16	1.0313798242372396	0.0	1.861696433641227E-4	0.0	2.79254465046184E-4
17	1.0656350386162383	0.0	1.861696433641227E-4	0.0	2.79254465046184E-4
18	1.1073370387298018	0.0	1.861696433641227E-4	0.0	2.79254465046184E-4
19	1.166632070141275	0.0	1.861696433641227E-4	0.0	2.79254465046184E-4
20	1.2342116506824514	0.0	1.861696433641227E-4	0.0	2.79254465046184E-4
21	1.3303682714800207	0.0	1.861696433641227E-4	0.0	3.723392867282454E-4
22	1.4423493119635404	0.0	1.861696433641227E-4	0.0	4.654241084103067E-4
23	1.5842105802070021	0.0	1.861696433641227E-4	0.0	4.654241084103067E-4
24	1.7719626655397198	0.0	1.861696433641227E-4	0.0	4.654241084103067E-4
25	1.9948077286465746	0.0	2.79254465046184E-4	0.0	4.654241084103067E-4
26	2.272014327615753	0.0	2.79254465046184E-4	0.0	5.58508930092368E-4
27	2.6046063954857583	0.0	2.79254465046184E-4	0.0	5.58508930092368E-4
28	2.9900706420711747	0.0	2.79254465046184E-4	0.0	5.58508930092368E-4
29	3.4469309468867317	0.0	2.79254465046184E-4	0.0	5.58508930092368E-4
30	3.9900808814015596	0.0	2.79254465046184E-4	0.0	5.58508930092368E-4
31	4.619055021507248	0.0	2.79254465046184E-4	0.0	5.58508930092368E-4
32	5.3028561215836705	0.0	2.79254465046184E-4	0.0	5.58508930092368E-4
33	6.090446797835591	0.0	2.79254465046184E-4	0.0	5.58508930092368E-4
34	6.962558492174824	0.0	2.79254465046184E-4	0.0	5.58508930092368E-4
35	7.906624753674291	0.0	2.79254465046184E-4	0.0	5.58508930092368E-4
36	8.97858956016491	0.0	2.79254465046184E-4	0.0	6.515937517744294E-4
37	10.111897264144005	0.0	2.79254465046184E-4	0.0	6.515937517744294E-4
38	11.312970718307643	2.79254465046184E-4	2.79254465046184E-4	0.0	6.515937517744294E-4
39	12.614948119174635	2.79254465046184E-4	2.79254465046184E-4	0.0	6.515937517744294E-4
40	13.990741783635503	2.79254465046184E-4	2.79254465046184E-4	0.0	6.515937517744294E-4
41	15.486894122531274	2.79254465046184E-4	2.79254465046184E-4	0.0	6.515937517744294E-4
42	17.086742952780863	2.79254465046184E-4	2.79254465046184E-4	0.0	6.515937517744294E-4
43	18.649637108822674	2.79254465046184E-4	2.79254465046184E-4	0.0	7.446785734564908E-4
44	20.31678626514839	2.79254465046184E-4	2.79254465046184E-4	0.0	7.446785734564908E-4
45	21.997246550974644	2.79254465046184E-4	2.79254465046184E-4	0.0	7.446785734564908E-4
46	23.749754488782813	2.79254465046184E-4	2.79254465046184E-4	0.0	7.446785734564908E-4
47	25.505985819458264	2.79254465046184E-4	2.79254465046184E-4	0.0	7.446785734564908E-4
48	27.242669337580484	2.79254465046184E-4	2.79254465046184E-4	0.0	7.446785734564908E-4
49	29.012491052221517	2.79254465046184E-4	2.79254465046184E-4	0.0	7.446785734564908E-4
50	30.80316376691933	2.79254465046184E-4	2.79254465046184E-4	0.0	7.446785734564908E-4
>>END_MODULE