##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-34B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 576228 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 57 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.55627980591016 40.0 40.0 40.0 40.0 40.0 2 39.34858771180852 40.0 40.0 40.0 40.0 40.0 3 39.2902323385882 40.0 40.0 40.0 40.0 40.0 4 39.17286907265874 40.0 40.0 40.0 40.0 40.0 5 39.2195242161089 40.0 40.0 40.0 40.0 40.0 6 39.36041983381578 40.0 40.0 40.0 40.0 40.0 7 39.37725344828783 40.0 40.0 40.0 40.0 40.0 8 39.37156819869912 40.0 40.0 40.0 40.0 40.0 9 39.38861700576855 40.0 40.0 40.0 40.0 40.0 10-11 39.40419764398814 40.0 40.0 40.0 40.0 40.0 12-13 39.32063697008823 40.0 40.0 40.0 40.0 40.0 14-15 39.353929347411096 40.0 40.0 40.0 40.0 40.0 16-17 39.4646112302769 40.0 40.0 40.0 40.0 40.0 18-19 39.49574126908099 40.0 40.0 40.0 40.0 40.0 20-21 39.47082404881401 40.0 40.0 40.0 40.0 40.0 22-23 39.51837467113712 40.0 40.0 40.0 40.0 40.0 24-25 39.49363099328738 40.0 40.0 40.0 40.0 40.0 26-27 39.47336123895403 40.0 40.0 40.0 40.0 40.0 28-29 39.48924037013127 40.0 40.0 40.0 40.0 40.0 30-31 39.46818586490201 40.0 40.0 40.0 40.0 40.0 32-33 39.47209373519791 40.0 40.0 40.0 40.0 40.0 34-35 39.47058402699072 40.0 40.0 40.0 40.0 40.0 36-37 39.446396754277195 40.0 40.0 40.0 40.0 40.0 38-39 39.38434110902271 40.0 40.0 40.0 40.0 40.0 40-41 39.43366813661815 40.0 40.0 40.0 40.0 40.0 42-43 39.41320300831276 40.0 40.0 40.0 40.0 40.0 44-45 39.310298903388244 40.0 40.0 40.0 40.0 40.0 46-47 39.30077579976071 40.0 40.0 40.0 40.0 40.0 48-49 39.3880440638535 40.0 40.0 40.0 40.0 40.0 50-51 39.3367346504507 40.0 40.0 40.0 40.0 40.0 52-53 39.29931217043945 40.0 40.0 40.0 40.0 40.0 54-55 39.212688224296144 40.0 40.0 40.0 40.0 40.0 56-57 39.22207595408682 40.0 40.0 40.0 40.0 40.0 58-59 39.16789831760801 40.0 40.0 40.0 40.0 40.0 60-61 39.086169921096534 40.0 40.0 40.0 40.0 40.0 62-63 39.35625959141595 40.0 40.0 40.0 40.0 40.0 64-65 39.36777242763439 40.0 40.0 40.0 40.0 40.0 66-67 39.361001343805285 40.0 40.0 40.0 40.0 40.0 68-69 39.37703894823021 40.0 40.0 40.0 40.0 40.0 70-71 39.365813974505954 40.0 40.0 40.0 40.0 40.0 72-73 39.38505000306537 40.0 40.0 40.0 40.0 40.0 74-75 39.368702581894595 40.0 40.0 40.0 40.0 40.0 76-77 39.35807861657749 40.0 40.0 40.0 40.0 40.0 78-79 39.35330048920876 40.0 40.0 40.0 40.0 40.0 80-81 39.36541408705037 40.0 40.0 40.0 40.0 40.0 82-83 39.357330006598396 40.0 40.0 40.0 40.0 40.0 84-85 39.36345993293601 40.0 40.0 40.0 40.0 40.0 86-87 39.32393635966813 40.0 40.0 40.0 40.0 40.0 88-89 39.383390167159675 40.0 40.0 40.0 40.0 40.0 90-91 39.46308653558781 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.003334901431628623 1101 2 -0.03038151812005907 1101 3 0.12314367622888511 1101 4 -0.08098001808125588 1101 5 0.1363596341297324 1101 6 0.10306015798284562 1101 7 0.018321070303734643 1101 8 -0.06226384549637487 1101 9 0.12476407647570653 1101 10-11 0.05447094715508882 1101 12-13 -0.025114894792636733 1101 14-15 -0.01687530720981556 1101 16-17 0.10816446110658262 1101 18-19 0.03703906540844315 1101 20-21 0.08770543634766881 1101 22-23 -0.11033316966738482 1101 24-25 0.03115759035825505 1101 26-27 0.08133107956513186 1101 28-29 0.11313495049086697 1101 30-31 0.08732213929349797 1101 32-33 0.0016991042745928553 1101 34-35 0.01615043001734051 1101 36-37 -0.052885959285859485 1101 38-39 0.09873963018738152 1101 40-41 -0.023654319087171416 1101 42-43 -0.03297645162581375 1101 44-45 0.0957479647981998 1101 46-47 -0.004958750744002316 1101 48-49 0.03266402972387539 1101 50-51 0.029481121413610367 1101 52-53 0.12842509631212806 1101 54-55 -0.0586347260931106 1101 56-57 0.05933125046875176 1101 58-59 0.07771746831416948 1101 60-61 -0.11006227920385925 1101 62-63 -0.13785561046476147 1101 64-65 -0.16906601703441027 1101 66-67 -0.10916514883145823 1101 68-69 -0.0027001600120257763 1101 70-71 0.030562026796118857 1101 72-73 0.02931589746247454 1101 74-75 0.14942072768411663 1101 76-77 -0.19715132363750598 1101 78-79 0.15348353355965827 1101 80-81 0.19122352484234995 1101 82-83 0.3373894071124681 1101 84-85 0.13563103325313364 1101 86-87 0.4515115141453663 1101 88-89 0.5646345294781696 1101 90-91 0.4389276115129377 1102 1 0.007400976937361747 1102 2 0.09632233308973781 1102 3 0.11188425510133726 1102 4 -0.026274677538637548 1102 5 -0.08019033290495514 1102 6 -0.24139520715164764 1102 7 0.020018519496105114 1102 8 -0.07275421157795847 1102 9 -0.016763188801945716 1102 10-11 0.032831721770740785 1102 12-13 -0.06348797754084501 1102 14-15 -0.04151270913700955 1102 16-17 -0.0565742837403036 1102 18-19 -0.02464305146028778 1102 20-21 -0.021017485150998994 1102 22-23 -0.0147776737284957 1102 24-25 -0.04427809233255431 1102 26-27 -0.024725350865054452 1102 28-29 -0.03138967837504225 1102 30-31 -0.04805756334397415 1102 32-33 0.03779777820036401 1102 34-35 0.0412871510020949 1102 36-37 -0.0925194945660408 1102 38-39 -0.12034628768246591 1102 40-41 0.07177780313364934 1102 42-43 -0.13750257888929696 1102 44-45 -0.02595480598014177 1102 46-47 -0.0012110898866879438 1102 48-49 -0.0013012748087888326 1102 50-51 -0.027653314824235053 1102 52-53 0.09460767954630711 1102 54-55 -0.10163570202407612 1102 56-57 -0.0428923124375018 1102 58-59 0.0031444892395882107 1102 60-61 -0.05919832609541231 1102 62-63 -0.056848565460562384 1102 64-65 0.038940606407571465 1102 66-67 0.05202621551750042 1102 68-69 -0.05925421940232667 1102 70-71 -0.16331789679863817 1102 72-73 0.015491716860537963 1102 74-75 -0.27190596446256166 1102 76-77 -0.32537238311477523 1102 78-79 -0.19367178593173406 1102 80-81 -0.5097326618281244 1102 82-83 -0.1843697936058959 1102 84-85 0.03180971525225118 1102 86-87 -1.1222289134033403 1102 88-89 0.5646345294781696 1102 90-91 0.4389276115129377 1103 1 -0.012525783324939255 1103 2 0.0036854712154763547 1103 3 0.056050720362712525 1103 4 -0.043589949005053086 1103 5 0.04062891202860186 1103 6 0.10801465540619404 1103 7 -0.06065207636842729 1103 8 -0.061998770245125456 1103 9 -0.0018647808210303651 1103 10-11 0.008143022561760915 1103 12-13 -0.03218999422597335 1103 14-15 0.006786273626687489 1103 16-17 -0.06081137246471968 1103 18-19 -0.0022595454767468937 1103 20-21 0.006903270441888765 1103 22-23 0.009859178574025407 1103 24-25 0.0037608582541466262 1103 26-27 -0.02549906623830367 1103 28-29 -0.04862119714641011 1103 30-31 -0.09886912695893102 1103 32-33 0.03898422873668039 1103 34-35 -0.05213210384676614 1103 36-37 -0.013998155978519833 1103 38-39 -0.0042982072913773095 1103 40-41 -0.01778233051355471 1103 42-43 -0.007868811148988186 1103 44-45 0.03450682286204909 1103 46-47 -0.12112632741867202 1103 48-49 0.03680204418115096 1103 50-51 0.04828956248250904 1103 52-53 0.06599270603702223 1103 54-55 0.005274534780532747 1103 56-57 0.11233912841503013 1103 58-59 -0.0920791037679436 1103 60-61 0.055828410363893965 1103 62-63 -0.0671772406395732 1103 64-65 -0.06689395705478063 1103 66-67 0.05969719541594287 1103 68-69 0.0806734665480846 1103 70-71 0.03836994570579577 1103 72-73 0.06550624158315799 1103 74-75 0.14337362697030187 1103 76-77 -0.05874198290871391 1103 78-79 -0.12019365406180071 1103 80-81 -0.08235507047537993 1103 82-83 0.18751180351799235 1103 84-85 -0.5500534905563867 1103 86-87 -0.3365048393415435 1103 88-89 -0.0976024583402122 1103 90-91 0.4389276115129377 1104 1 0.09597629347262426 1104 2 0.02794296676910335 1104 3 0.0541397462854718 1104 4 -0.15538896390807366 1104 5 0.01725658966774546 1104 6 -0.026803550098520645 1104 7 -0.03476720591582705 1104 8 0.13061272895934906 1104 9 -0.009060231046447598 1104 10-11 -0.12489468011301597 1104 12-13 0.09959143440293161 1104 14-15 -0.09971107729916184 1104 16-17 0.011590013343052874 1104 18-19 -0.06025440553462147 1104 20-21 0.030132621316184327 1104 22-23 -0.020963286387221558 1104 24-25 -0.008010460806254116 1104 26-27 -0.026777996967837225 1104 28-29 0.03228425768455878 1104 30-31 -0.012460441792683241 1104 32-33 0.00946117054786555 1104 34-35 -0.13034918214977864 1104 36-37 -0.1104518232800018 1104 38-39 -0.008220138370603536 1104 40-41 0.047451086968266054 1104 42-43 -0.037226218766889474 1104 44-45 -0.11538537871891918 1104 46-47 0.008358586867409201 1104 48-49 -0.022562897980520802 1104 50-51 -0.12341511675587924 1104 52-53 0.025347321401469003 1104 54-55 0.013662944359943197 1104 56-57 -0.012664409030890056 1104 58-59 -0.02695113263315818 1104 60-61 0.06799360338287386 1104 62-63 -0.032829837820536056 1104 64-65 0.0375661225984274 1104 66-67 -0.044461253697939185 1104 68-69 0.10783370349302857 1104 70-71 -0.02084281939970367 1104 72-73 -0.03382029532370012 1104 74-75 -0.07204841576105991 1104 76-77 -0.17780504254385932 1104 78-79 -0.07798719359302453 1104 80-81 -0.09622586787020282 1104 82-83 -0.09115915198223945 1104 84-85 0.2463405295887 1104 86-87 0.08584051847437024 1104 88-89 -0.1848127807650144 1104 90-91 -1.366025948858578 1201 1 0.13946260336683736 1201 2 0.2089922822638144 1201 3 0.12384105306829696 1201 4 0.2002659831983351 1201 5 0.061391623893008784 1201 6 0.017810637829292375 1201 7 0.10234698201198711 1201 8 0.0709783170116225 1201 9 0.008271754018892352 1201 10-11 0.06945815124977628 1201 12-13 0.06586399196361015 1201 14-15 0.08705463520860945 1201 16-17 0.13054769847062175 1201 18-19 0.10111616073793783 1201 20-21 -0.037056239915919775 1201 22-23 0.17938052195129472 1201 24-25 0.08347444896350709 1201 26-27 0.043233670735958185 1201 28-29 0.1226335109515162 1201 30-31 0.061399234622996346 1201 32-33 0.08450572592401784 1201 34-35 0.08903670763888982 1201 36-37 0.0316247630337827 1201 38-39 0.1609753654196524 1201 40-41 0.0690803484286846 1201 42-43 0.15479771700383083 1201 44-45 0.11586802600085377 1201 46-47 0.056580744235795066 1201 48-49 0.08095224546233482 1201 50-51 0.13422025953884287 1201 52-53 0.002341185701418169 1201 54-55 0.3183717405441868 1201 56-57 0.16395444311449126 1201 58-59 0.22268349123454811 1201 60-61 0.31398328194681113 1201 62-63 0.13853265843157203 1201 64-65 0.1301467503942959 1201 66-67 0.03636860414928833 1201 68-69 0.12605854327629373 1201 70-71 0.0014443799285714931 1201 72-73 -0.03938911895400565 1201 74-75 -0.021246671875736922 1201 76-77 -0.06766822410086348 1201 78-79 -0.014351207360242313 1201 80-81 -0.07454428815561442 1201 82-83 -0.18572093840448645 1201 84-85 -0.38482009456641464 1201 86-87 -0.13019688055271672 1201 88-89 -0.9895255961105249 1201 90-91 0.4389276115129377 1202 1 0.03670932006477301 1202 2 -0.007665785278398118 1202 3 0.11848145331845217 1202 4 0.007246908776821215 1202 5 -0.051632140960023776 1202 6 -0.042611501007478125 1202 7 -0.07809235507369294 1202 8 0.09815311375958657 1202 9 -0.06464413090918697 1202 10-11 0.02535246019286319 1202 12-13 0.0016988887741149483 1202 14-15 -0.1371808244536794 1202 16-17 -0.052319718665089 1202 18-19 0.008319100075027563 1202 20-21 -0.10620414485651253 1202 22-23 0.061624111694889905 1202 24-25 -0.05813046035482472 1202 26-27 -0.030874365536710968 1202 28-29 0.0060598711766530755 1202 30-31 0.053599047005732814 1202 32-33 0.10920582068042961 1202 34-35 -0.01707396529107541 1202 36-37 -0.15320430121610684 1202 38-39 0.0033077130669667554 1202 40-41 -0.14179503092533707 1202 42-43 -0.08491695067653637 1202 44-45 0.0355711559060623 1202 46-47 0.012718256308012599 1202 48-49 -0.09300282546339389 1202 50-51 -0.08619431647938569 1202 52-53 -0.08164833730209864 1202 54-55 -0.10632638263833627 1202 56-57 -0.14355941869332867 1202 58-59 0.07853267694482469 1202 60-61 0.15010130158371737 1202 62-63 0.13593633831136742 1202 64-65 0.04305030208368521 1202 66-67 0.015822014869343093 1202 68-69 -0.16845245058662073 1202 70-71 -0.15475847937561582 1202 72-73 -0.08460276563651092 1202 74-75 -0.28725472125847773 1202 76-77 -0.28770724284056115 1202 78-79 -0.2150938293879463 1202 80-81 -0.04930354068471843 1202 82-83 -0.21437338726543942 1202 84-85 0.19124165272216231 1202 86-87 0.08935880906879845 1202 88-89 0.17583901127088808 1202 90-91 0.22271139529672013 1203 1 -0.04900312872038626 1203 2 0.09695567790244297 1203 3 0.003498373940573174 1203 4 -0.05693806664589829 1203 5 -0.04351491816306208 1203 6 -0.06500867681244671 1203 7 -0.10786329836184194 1203 8 0.03530074691816054 1203 9 0.04500851414350393 1203 10-11 -0.09713063379695797 1203 12-13 -0.1341360080363927 1203 14-15 0.06634591246718458 1203 16-17 -0.03516881243280068 1203 18-19 1.2706416472951787E-4 1203 20-21 -0.042764962657358296 1203 22-23 -0.053976175868022835 1203 24-25 -0.03482383764085739 1203 26-27 -0.1010069834696452 1203 28-29 -0.0730012242509801 1203 30-31 0.040441290697756926 1203 32-33 -0.02130373554846443 1203 34-35 0.0024685693907215978 1203 36-37 0.08700537066346214 1203 38-39 0.0420651963297658 1203 40-41 -0.10089683819467155 1203 42-43 0.0420603003678508 1203 44-45 -0.041952995601370446 1203 46-47 -0.030894216475665814 1203 48-49 -0.1906378425575923 1203 50-51 -0.1556755817353519 1203 52-53 -0.0027853426727375563 1203 54-55 0.15697040598001166 1203 56-57 -0.1159924198222484 1203 58-59 0.11778029253768807 1203 60-61 -0.18802946460059644 1203 62-63 -0.07358955464770389 1203 64-65 0.0894196014290003 1203 66-67 0.04989537555182011 1203 68-69 0.16413875062348637 1203 70-71 0.09473162295236648 1203 72-73 -0.03465129053216032 1203 74-75 0.15633583324792255 1203 76-77 0.010026864990571482 1203 78-79 -0.030882948066661697 1203 80-81 0.44634058169853574 1203 82-83 0.5075408635472911 1203 84-85 -0.0813525088741045 1203 86-87 0.038678101710623025 1203 88-89 0.355679305597576 1203 90-91 0.2036334938658797 1204 1 -0.003297335471081908 1204 2 -0.08136322232333981 1204 3 -0.24560848821610648 1204 4 -0.08211169264264129 1204 5 -0.16773681647396188 1204 6 -0.16474287899027473 1204 7 -0.11643742165773574 1204 8 -0.10568070439510535 1204 9 -0.15029858237254246 1204 10-11 -0.1492727356003769 1204 12-13 -0.21853967776116434 1204 14-15 -0.13108756662624899 1204 16-17 -0.05160826483212588 1204 18-19 -0.19038995547001036 1204 20-21 -0.043256545726322315 1204 22-23 -0.15808889700894468 1204 24-25 -0.10189634614352627 1204 26-27 -0.13691541183284528 1204 28-29 -8.871312503160311E-4 1204 30-31 -0.004327065071194625 1204 32-33 -0.19420584143249187 1204 34-35 -0.07961477585387655 1204 36-37 -0.07695213545830626 1204 38-39 -0.08240834085470539 1204 40-41 -0.05428485111539061 1204 42-43 -0.11619724922130104 1204 44-45 0.010362192544498328 1204 46-47 -0.0016670068314965647 1204 48-49 0.01434082321195973 1204 50-51 0.08325938972677704 1204 52-53 -0.1028947159113045 1204 54-55 -0.22113360800837967 1204 56-57 -0.07548343488609532 1204 58-59 -0.28209178482796204 1204 60-61 -0.22137606116160669 1204 62-63 -0.010845645796585757 1204 64-65 0.02569927118772597 1204 66-67 0.00790337074278824 1204 68-69 -0.343440854567703 1204 70-71 -0.170934797727611 1204 72-73 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0.12805871168642113 2401 40-41 0.0466191073221367 2401 42-43 0.05544902965529275 2401 44-45 0.019148308695029925 2401 46-47 0.05974069036891194 2401 48-49 0.08671367817691333 2401 50-51 0.08284915452207997 2401 52-53 0.05160767971609914 2401 54-55 0.12719448123150556 2401 56-57 0.1274641014330271 2401 58-59 -0.047267003733544755 2401 60-61 0.23009719297958497 2401 62-63 0.13462396677888933 2401 64-65 0.009311182859860878 2401 66-67 0.037145985491498834 2401 68-69 0.06751212966530318 2401 70-71 0.06468816655804233 2401 72-73 0.22040518755765248 2401 74-75 -0.029368979069502643 2401 76-77 0.11739559108665532 2401 78-79 0.1630883106099148 2401 80-81 -0.08274517480287358 2401 82-83 0.2274614879798662 2401 84-85 0.4789476321010966 2401 86-87 0.25633714059847534 2401 88-89 -0.24984510853087727 2401 90-91 -0.3524702379494329 2402 1 -0.010585316221749963 2402 2 0.02065135192253109 2402 3 0.157141660828934 2402 4 0.07105256571352925 2402 5 0.040248566389500695 2402 6 -0.09276205969290885 2402 7 0.015216313475285403 2402 8 0.08364895749782875 2402 9 -0.057479765382694836 2402 10-11 0.11572977350316904 2402 12-13 0.012287554375944865 2402 14-15 0.0224438357696215 2402 16-17 0.05027420618451117 2402 18-19 0.05070358476785941 2402 20-21 0.040495185984028126 2402 22-23 0.008112406009267659 2402 24-25 0.03941121848196616 2402 26-27 0.0436199493708358 2402 28-29 0.14146181857189788 2402 30-31 0.043015644160156796 2402 32-33 0.04216547239545321 2402 34-35 0.05639313193536566 2402 36-37 0.13008954396946137 2402 38-39 -0.4028055649911906 2402 40-41 0.03087957639930039 2402 42-43 0.06309666619246457 2402 44-45 0.12417265175654535 2402 46-47 0.13301677915733734 2402 48-49 0.18912022284372654 2402 50-51 0.09449027321412018 2402 52-53 0.18450093701628134 2402 54-55 0.07766427326760805 2402 56-57 0.0789294255275621 2402 58-59 0.14794089043024172 2402 60-61 0.13660158305546588 2402 62-63 0.1309205324370879 2402 64-65 0.10992827815655914 2402 66-67 0.10097449891510024 2402 68-69 0.14978685626800115 2402 70-71 0.36602468650536935 2402 72-73 0.2048437468622879 2402 74-75 0.06090452004396241 2402 76-77 0.10540185553755776 2402 78-79 0.3237841796609544 2402 80-81 0.36217777366211834 2402 82-83 0.19793088621747756 2402 84-85 0.20120288076046933 2402 86-87 0.4398837519293721 2402 88-89 0.5646345294781696 2402 90-91 0.4389276115129377 2403 1 -0.015547731956999655 2403 2 0.02696024275991249 2403 3 0.10587596349549244 2403 4 0.10648440304215967 2403 5 -0.02757254698434508 2403 6 0.1312029207874943 2403 7 0.060767974203074004 2403 8 0.0929845182059239 2403 9 0.07344056430828516 2403 10-11 0.08733826149322965 2403 12-13 0.14588236587265158 2403 14-15 0.17193864990773733 2403 16-17 0.0633382359074659 2403 18-19 0.11613797975493867 2403 20-21 0.08028298837989922 2403 22-23 0.010695404817631982 2403 24-25 0.0834497590232175 2403 26-27 0.08998550812686545 2403 28-29 0.04616187567361152 2403 30-31 0.08833194018110646 2403 32-33 0.09272491575573838 2403 34-35 0.16414877264463001 2403 36-37 0.08069718447917751 2403 38-39 0.1310374608123297 2403 40-41 0.06792699104755684 2403 42-43 0.04642816332139432 2403 44-45 0.09536053784292875 2403 46-47 0.12174548652978956 2403 48-49 0.09155724176874713 2403 50-51 0.051533920278686196 2403 52-53 0.04788095054659891 2403 54-55 0.14893478540906102 2403 56-57 0.12172267149987448 2403 58-59 0.008368790354460032 2403 60-61 0.15232758493148424 2403 62-63 0.1758646720421595 2403 64-65 -0.01854225104591478 2403 66-67 0.13470845224346562 2403 68-69 0.12930166152992228 2403 70-71 0.09407872627744496 2403 72-73 0.22168398053204186 2403 74-75 -0.059941974922438135 2403 76-77 0.22879774903659467 2403 78-79 0.1514316286494548 2403 80-81 0.07475054937656722 2403 82-83 0.20135284052975067 2403 84-85 0.09734865606316845 2403 86-87 0.3527249839569606 2403 88-89 -0.10662192491081157 2403 90-91 0.07529124787657793 2404 1 0.028588743184471355 2404 2 -0.05645116107096726 2404 3 -0.06516893390521261 2404 4 0.04228183209108494 2404 5 0.03259223179574633 2404 6 -0.16788410815420463 2404 7 0.1281036038096417 2404 8 0.05573407037680056 2404 9 -0.038394550800909144 2404 10-11 0.018687417424324337 2404 12-13 0.015408064911937913 2404 14-15 0.08723157832628203 2404 16-17 -0.014170060973583531 2404 18-19 -0.01139035250565712 2404 20-21 -0.007115944648880657 2404 22-23 0.005532280527070554 2404 24-25 -0.010466606181957161 2404 26-27 0.0035555335236239216 2404 28-29 -0.024965816013313713 2404 30-31 0.0128397469981536 2404 32-33 -0.058704353122358555 2404 34-35 4.3040989535114704E-4 2404 36-37 -0.006788121910744849 2404 38-39 0.07985350006865843 2404 40-41 -0.016565091574705093 2404 42-43 -0.006578703062785962 2404 44-45 0.06818216074714911 2404 46-47 0.036875432151880716 2404 48-49 0.07760020875743834 2404 50-51 -0.03767242773030688 2404 52-53 0.08775905892230185 2404 54-55 -0.021739984604685958 2404 56-57 0.019566694178124067 2404 58-59 0.08336606294209048 2404 60-61 0.043997160467100116 2404 62-63 0.029048116924592193 2404 64-65 -0.07381514293011548 2404 66-67 -0.1413095747570523 2404 68-69 -0.06589827205101528 2404 70-71 0.006101774415398609 2404 72-73 0.07361089811360699 2404 74-75 0.08593668219906192 2404 76-77 0.1126023319001277 2404 78-79 -0.09016955221506606 2404 80-81 -0.10873461945877949 2404 82-83 -0.02763374665354945 2404 84-85 0.020397847195205543 2404 86-87 0.10669948407017671 2404 88-89 -0.5301930567287272 2404 90-91 -0.44479331871961847 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 15.0 31 384.0 32 2557.0 33 4531.0 34 6019.0 35 7304.0 36 10291.0 37 15136.0 38 37617.0 39 244785.0 40 247589.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.834197574571174 11.81563547762344 27.508035013918104 30.84213193388728 2 30.916056373233996 16.356674055571276 25.194891761516015 27.532377809678714 3 28.66833961556884 18.462830685076046 19.869565519204205 32.99926418015091 4 27.875077226375673 20.017597201107897 19.472674010981763 32.634651561534675 5 28.262423901650042 20.198775484703972 19.512415224529178 32.026385389116804 6 28.57254420125367 22.765120750813914 22.03953990434342 26.622795143588995 7 27.12311793248506 22.06366230033945 21.94252969310759 28.870690074067905 8 26.63494311279563 22.91141700854523 21.941488438604164 28.51215144005498 9 26.47233386784398 22.978751466433426 22.71514053464948 27.83377413107312 10-11 26.585657066300143 21.629806257245395 22.349052805486718 29.435483870967744 12-13 27.06263840007775 21.590065043697983 22.100366521585205 29.246930034639067 14-15 27.510898463802526 21.253479525465615 21.88847123013807 29.347150780593793 16-17 27.68062294786092 21.085143380745123 21.409320616144996 29.824913055248963 18-19 27.70509242869142 21.399341927153834 21.52212318734945 29.373442456805293 20-21 27.743965930152648 21.44784703277175 21.45140465232512 29.35678238475048 22-23 27.618147677655376 21.08149898998313 21.52220995855807 29.778143373803424 24-25 27.784921940620727 21.378082981042226 21.527329459866582 29.309665618470465 26-27 27.948572440075804 21.48576605093817 21.44793380398037 29.11772770500566 28-29 27.84991357587622 21.198987206453 21.55145185586261 29.399647361808174 30-31 27.792316876770144 21.390379435774975 21.76566751818737 29.051636169267507 32-33 27.839516606101583 21.387046113975043 21.765049719710415 29.008387560212963 34-35 27.879422506945616 21.06068975833857 21.90966330327397 29.150224431441853 36-37 27.94724971564126 21.34651163437445 21.86693216085759 28.8393064891267 38-39 28.224968606733746 21.389918276780687 21.735843771374523 28.649269345111044 40-41 27.899773035682617 21.186292072588728 21.890798081464048 29.023136810264603 42-43 27.96029667048029 21.36585955457749 22.01157006537337 28.662273709568854 44-45 28.041205280740538 21.438418186735518 21.98753724209745 28.532839290426494 46-47 28.011018698046534 21.18950173207093 22.054381711919284 28.745097857963252 48-49 28.176268646483248 21.373301859995884 22.05378218752755 28.396647305993323 50-51 28.255138595140078 21.43947123911392 21.94235182354482 28.36303834220118 52-53 28.19515486611543 21.188573213048432 21.98210782981108 28.634164091025056 54-55 28.240694974912007 21.42889238373399 22.12206994682843 28.208342694525573 56-57 28.363841380769834 21.371303211381917 21.9961648872325 28.268690520615753 58-59 28.284760977795827 21.182892074139946 21.979049232902227 28.553297715161996 60-61 28.556321725196398 21.46244614953709 21.91973446791646 28.061497657350053 62-63 28.554227427150025 21.403201976611214 21.924691780536058 28.117878815702706 64-65 28.43826542933039 21.194328492082658 21.930520578190222 28.43688550039673 66-67 28.55048594185015 21.337844315041252 22.051204911429068 28.060464831679532 68-69 28.5614419534966 21.42329278766453 21.935748953545104 28.07951630529376 70-71 28.562463796099635 21.238977923666088 21.909313252236593 28.289245027997683 72-73 28.6256585347945 21.434366796996898 21.771389760816493 28.16858490739211 74-75 28.607741629870244 21.525486812498944 21.76318327158301 28.10358828604781 76-77 28.75160493827161 21.19358024691358 21.674567901234568 28.380246913580248 78-79 28.784589386720494 21.362686882437156 21.59680334217382 28.255920388668525 80-81 28.960244212087925 21.405645978060853 21.35571944139338 28.27839036845784 82-83 28.912569238089254 20.73686383998707 21.44069089962385 28.909876022299823 84-85 29.38524009632183 21.055054535152745 20.960621370225223 28.599083998300202 86-87 29.630746548720012 20.26035383996783 20.520037528481435 29.58886208283072 88-89 29.008341056533826 19.492449435752057 21.22880662923186 30.270402878482255 90-91 25.15179514255544 21.7331045406547 23.812038014783525 29.30306230200634 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 1.0 7 3.0 8 2.5 9 2.5 10 6.0 11 6.5 12 5.5 13 12.0 14 23.5 15 33.0 16 39.5 17 49.5 18 74.5 19 113.0 20 156.5 21 216.0 22 315.5 23 444.5 24 574.5 25 715.5 26 908.0 27 1162.0 28 1444.5 29 1735.5 30 2086.0 31 2526.5 32 3058.0 33 3574.5 34 4157.5 35 4834.0 36 5606.5 37 6481.5 38 7514.5 39 8737.5 40 10078.0 41 11361.0 42 12617.5 43 14097.0 44 15693.5 45 17370.5 46 19313.0 47 21337.0 48 23101.0 49 25131.0 50 27325.0 51 29249.5 52 30956.5 53 32715.5 54 34474.5 55 36128.5 56 37871.0 57 39454.5 58 40740.5 59 41564.0 60 41944.5 61 42061.5 62 41871.5 63 41036.5 64 39536.0 65 37596.0 66 34771.0 67 31736.5 68 28656.5 69 25252.5 70 21926.0 71 18668.0 72 15570.0 73 12706.0 74 10201.0 75 8185.0 76 6522.0 77 4974.0 78 3755.0 79 2842.0 80 2156.5 81 1644.0 82 1205.5 83 861.0 84 620.5 85 453.0 86 323.0 87 220.0 88 152.0 89 111.0 90 82.0 91 61.0 92 42.0 93 25.5 94 19.5 95 16.0 96 8.5 97 3.0 98 1.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.024122395996029347 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.07141720493190921 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.671260637477697E-4 74-75 4.2294564725487127E-4 76-77 4.938247218532254E-4 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.001180400627973134 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 5269.0 32-33 12575.0 34-35 14836.0 36-37 17203.0 38-39 19264.0 40-41 21604.0 42-43 22963.0 44-45 23746.0 46-47 24267.0 48-49 24577.0 50-51 25109.0 52-53 24818.0 54-55 24434.0 56-57 23973.0 58-59 23435.0 60-61 22985.0 62-63 22273.0 64-65 21551.0 66-67 21052.0 68-69 20044.0 70-71 19461.0 72-73 18087.0 74-75 17386.0 76-77 16175.0 78-79 15349.0 80-81 14628.0 82-83 13582.0 84-85 12772.0 86-87 11697.0 88-89 10949.0 90-91 10164.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.83095546660932 #Duplication Level Percentage of deduplicated Percentage of total 1 99.84246438717877 99.67368615912972 2 0.14858686155158438 0.2966713671695893 3 0.006385223792830042 0.019123289763190505 4 0.002280641230579387 0.009107143725011355 5 2.8288624623483985E-4 0.001412040212499329 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0017354241723762122 0.0 0.0 0.0 0.0 2 0.0017354241723762122 0.0 0.0 0.0 0.0 3 0.0017354241723762122 0.0 0.0 0.0 0.0 4 0.0017354241723762122 0.0 0.0 0.0 0.0 5 0.0017354241723762122 0.0 0.0 0.0 0.0 6 0.0020825090068514545 0.0 1.7354241723762122E-4 0.0 0.0 7 0.002429593841326697 0.0 1.7354241723762122E-4 0.0 3.4708483447524243E-4 8 0.008156493610168198 0.0 1.7354241723762122E-4 0.0 3.4708483447524243E-4 9 0.008156493610168198 0.0 1.7354241723762122E-4 0.0 3.4708483447524243E-4 10-11 0.008156493610168198 0.0 1.7354241723762122E-4 0.0 3.4708483447524243E-4 12-13 0.008156493610168198 0.0 1.7354241723762122E-4 0.0 3.4708483447524243E-4 14-15 0.008156493610168198 0.0 1.7354241723762122E-4 0.0 3.4708483447524243E-4 16-17 0.008156493610168198 0.0 1.7354241723762122E-4 0.0 3.4708483447524243E-4 18-19 0.008156493610168198 0.0 1.7354241723762122E-4 0.0 3.4708483447524243E-4 20-21 0.008156493610168198 0.0 1.7354241723762122E-4 0.0 4.33856043094053E-4 22-23 0.008156493610168198 0.0 1.7354241723762122E-4 0.0 6.941696689504849E-4 24-25 0.008330036027405818 0.0 2.603136258564318E-4 0.0 6.941696689504849E-4 26-27 0.008330036027405818 0.0 3.4708483447524243E-4 0.0 8.677120861881061E-4 28-29 0.008330036027405818 0.0 3.4708483447524243E-4 0.0 8.677120861881061E-4 30-31 0.008330036027405818 0.0 3.4708483447524243E-4 0.0 8.677120861881061E-4 32-33 0.008850663279118681 0.0 3.4708483447524243E-4 0.0 8.677120861881061E-4 34-35 0.009024205696356303 0.0 3.4708483447524243E-4 0.0 8.677120861881061E-4 36-37 0.009024205696356303 0.0 3.4708483447524243E-4 0.0 0.0010412545034257272 38-39 0.009197748113593925 1.7354241723762122E-4 3.4708483447524243E-4 0.0 0.0010412545034257272 40-41 0.009284519322212735 1.7354241723762122E-4 3.4708483447524243E-4 0.0 0.0010412545034257272 42-43 0.009718375365306788 1.7354241723762122E-4 3.4708483447524243E-4 0.0 0.0010412545034257272 44-45 0.00989191778254441 1.7354241723762122E-4 3.4708483447524243E-4 0.0 0.0010412545034257272 46-47 0.010065460199782031 1.7354241723762122E-4 3.4708483447524243E-4 0.0 0.0010412545034257272 48-49 0.010239002617019651 1.7354241723762122E-4 3.4708483447524243E-4 0.0 0.0010412545034257272 50-51 0.010586087451494895 1.7354241723762122E-4 3.4708483447524243E-4 0.0 0.0010412545034257272 52-53 0.010586087451494895 1.7354241723762122E-4 3.4708483447524243E-4 0.0 0.0010412545034257272 54-55 0.010759629868732516 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