##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-34A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1919093 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 58 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.528439736896544 40.0 40.0 40.0 40.0 40.0 2 39.21145561992045 40.0 40.0 40.0 40.0 40.0 3 39.179836516521085 40.0 40.0 40.0 40.0 40.0 4 39.11960076973862 40.0 40.0 40.0 40.0 40.0 5 39.163383952731834 40.0 40.0 40.0 40.0 40.0 6 39.260188015901264 40.0 40.0 40.0 40.0 40.0 7 39.30295822036764 40.0 40.0 40.0 40.0 40.0 8 39.35602287122094 40.0 40.0 40.0 40.0 40.0 9 39.34416727068464 40.0 40.0 40.0 40.0 40.0 10-11 39.355141204725356 40.0 40.0 40.0 40.0 40.0 12-13 39.27747743335003 40.0 40.0 40.0 40.0 40.0 14-15 39.32357837791082 40.0 40.0 40.0 40.0 40.0 16-17 39.429992189018456 40.0 40.0 40.0 40.0 40.0 18-19 39.463860271492834 40.0 40.0 40.0 40.0 40.0 20-21 39.45089477164473 40.0 40.0 40.0 40.0 40.0 22-23 39.493951569830124 40.0 40.0 40.0 40.0 40.0 24-25 39.47302397538837 40.0 40.0 40.0 40.0 40.0 26-27 39.44193220443199 40.0 40.0 40.0 40.0 40.0 28-29 39.46422711145317 40.0 40.0 40.0 40.0 40.0 30-31 39.44246592153454 40.0 40.0 40.0 40.0 40.0 32-33 39.447338752483475 40.0 40.0 40.0 40.0 40.0 34-35 39.447467469160216 40.0 40.0 40.0 40.0 40.0 36-37 39.42622437400079 40.0 40.0 40.0 40.0 40.0 38-39 39.33078724455179 40.0 40.0 40.0 40.0 40.0 40-41 39.41244441392442 40.0 40.0 40.0 40.0 40.0 42-43 39.383633867897906 40.0 40.0 40.0 40.0 40.0 44-45 39.270092659615976 40.0 40.0 40.0 40.0 40.0 46-47 39.26215144736065 40.0 40.0 40.0 40.0 40.0 48-49 39.35744055347327 40.0 40.0 40.0 40.0 40.0 50-51 39.310336182751556 40.0 40.0 40.0 40.0 40.0 52-53 39.265124433318306 40.0 40.0 40.0 40.0 40.0 54-55 39.18645725339272 40.0 40.0 40.0 40.0 40.0 56-57 39.193527639459035 40.0 40.0 40.0 40.0 40.0 58-59 39.127992697908525 40.0 40.0 40.0 40.0 40.0 60-61 39.033206838874186 40.0 40.0 40.0 40.0 40.0 62-63 39.20727725469318 40.0 40.0 40.0 40.0 40.0 64-65 39.2115547592083 40.0 40.0 40.0 40.0 40.0 66-67 39.21688771479012 40.0 40.0 40.0 40.0 40.0 68-69 39.21966105748428 40.0 40.0 40.0 40.0 40.0 70-71 39.210690871863406 40.0 40.0 40.0 40.0 40.0 72-73 39.22306554777538 40.0 40.0 40.0 40.0 40.0 74-75 39.223201662117575 40.0 40.0 40.0 40.0 40.0 76-77 39.21179653174495 40.0 40.0 40.0 40.0 40.0 78-79 39.20612638126145 40.0 40.0 40.0 40.0 40.0 80-81 39.20023563361263 40.0 40.0 40.0 40.0 40.0 82-83 39.19447621844155 40.0 40.0 40.0 40.0 40.0 84-85 39.18658380548696 40.0 40.0 40.0 40.0 40.0 86-87 39.165392071251674 40.0 40.0 40.0 40.0 40.0 88-89 39.19347944925376 40.0 40.0 40.0 40.0 40.0 90-91 39.291883379780295 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.07106864477747621 1101 2 -0.1379422461509705 1101 3 -0.07350217969718642 1101 4 -0.14996865273779747 1101 5 -0.05418770709396625 1101 6 -0.09683041711397067 1101 7 -0.10645554009143154 1101 8 -0.056142173084055 1101 9 -0.025262305067307977 1101 10-11 -0.00587976848432703 1101 12-13 -0.05687438313378124 1101 14-15 0.0511678580754662 1101 16-17 0.0022282194967075952 1101 18-19 0.027577018693854427 1101 20-21 0.03584459045025312 1101 22-23 0.003870737676884062 1101 24-25 -0.005369995917071435 1101 26-27 0.017749816241881433 1101 28-29 0.02545969695369621 1101 30-31 -0.009128705854820396 1101 32-33 0.06842386026698932 1101 34-35 -0.07702351493519899 1101 36-37 -0.05644371926804581 1101 38-39 0.0429729072857441 1101 40-41 -0.06516898732041199 1101 42-43 0.030761163373739464 1101 44-45 -0.029427151791516337 1101 46-47 -0.005190841727348072 1101 48-49 -9.660438430358909E-4 1101 50-51 0.027580732949715525 1101 52-53 -9.376017267470615E-6 1101 54-55 0.013659510702694888 1101 56-57 -0.10750647572081817 1101 58-59 -0.030465177043744518 1101 60-61 0.0143261984419496 1101 62-63 -0.19753110822910713 1101 64-65 -0.3137785137365796 1101 66-67 -0.23585770116881832 1101 68-69 -0.2469916224545159 1101 70-71 -0.2145953235764111 1101 72-73 -0.17073080423610776 1101 74-75 -0.16896288708123564 1101 76-77 -0.07860005208407017 1101 78-79 -0.12115971721559049 1101 80-81 -0.1212777965256393 1101 82-83 -0.17339013180446727 1101 84-85 -0.31538303362408726 1101 86-87 -0.33225837621304777 1101 88-89 -0.7320068935056412 1101 90-91 0.06965081793747174 1102 1 -0.015246948616585598 1102 2 -0.14434774158511487 1102 3 0.022156124661059096 1102 4 0.01173251833800748 1102 5 -0.013046393656409805 1102 6 -0.05048938313017004 1102 7 -0.04777416904408227 1102 8 -0.12333155442897237 1102 9 -0.18116037839869392 1102 10-11 -0.1988583581440011 1102 12-13 -0.18863589927571667 1102 14-15 -0.2251899181563246 1102 16-17 -0.04056209639721686 1102 18-19 -0.2070154972249938 1102 20-21 -0.13401637567320535 1102 22-23 -0.09580330640246615 1102 24-25 -0.08309643929010235 1102 26-27 -0.14750011390692208 1102 28-29 -0.1677561786252184 1102 30-31 -0.05147089663808657 1102 32-33 -0.08037266065966264 1102 34-35 -0.08783574986872367 1102 36-37 -0.09656579582585323 1102 38-39 -0.025771139707053692 1102 40-41 -0.14984234276655428 1102 42-43 -0.07897771124979869 1102 44-45 -0.04140043472676069 1102 46-47 -0.18964685171022921 1102 48-49 -0.19929174877539424 1102 50-51 -0.12413175562502943 1102 52-53 -0.10569039628393284 1102 54-55 -0.20789298217852803 1102 56-57 -0.260073135997537 1102 58-59 -0.2761643379451968 1102 60-61 -0.12336297279902908 1102 62-63 -0.3911811699490997 1102 64-65 -0.19067060296858784 1102 66-67 -0.19974387735231858 1102 68-69 -0.3073025057090888 1102 70-71 -0.19579750119844874 1102 72-73 -0.15726001356910757 1102 74-75 -0.3730112288515244 1102 76-77 -0.4372266310853661 1102 78-79 -0.36116169487904415 1102 80-81 -0.20840403487346038 1102 82-83 0.08326126172433845 1102 84-85 -0.17804720738098467 1102 86-87 -0.05567807697438809 1102 88-89 -0.16005224408620222 1102 90-91 0.22161788262938842 1103 1 0.02515242831044162 1103 2 -0.017965365343250994 1103 3 -0.011044993016227522 1103 4 -0.10378890380999195 1103 5 -0.005415890915742239 1103 6 -0.06497823847446682 1103 7 -7.131571393230729E-4 1103 8 -0.03173206616244073 1103 9 -0.03947818843638373 1103 10-11 -0.06683941972330132 1103 12-13 0.015564356591021067 1103 14-15 -0.14687957535912943 1103 16-17 -0.08143319416185335 1103 18-19 -0.039750648389777155 1103 20-21 -0.014505734186400332 1103 22-23 -0.12087610530619486 1103 24-25 -0.12516724692970627 1103 26-27 -0.15700344873905436 1103 28-29 -0.03934105358239748 1103 30-31 -0.09981057311119912 1103 32-33 -0.07359354073868474 1103 34-35 -0.07691295830750278 1103 36-37 -0.09046776350108132 1103 38-39 -0.10233415914149901 1103 40-41 -0.10063526564879766 1103 42-43 -0.10215122338042448 1103 44-45 -0.09261161959849318 1103 46-47 -0.13330633838889838 1103 48-49 -0.22567226168322918 1103 50-51 -0.07620468884664433 1103 52-53 -0.04199603816535813 1103 54-55 -0.05227176902342734 1103 56-57 -0.12431471578243958 1103 58-59 -0.1288694990841961 1103 60-61 -0.0927019475308839 1103 62-63 -0.16453254983810695 1103 64-65 -0.1037817077468901 1103 66-67 -0.11397353393991949 1103 68-69 -0.042699360875843695 1103 70-71 -0.026180575218340607 1103 72-73 -0.10889551797649943 1103 74-75 -0.1638351406043057 1103 76-77 -0.15735894984222654 1103 78-79 -0.2465977500098333 1103 80-81 -0.30977082357323127 1103 82-83 -0.1528569977652694 1103 84-85 -0.35623584201902503 1103 86-87 -0.2629408870876233 1103 88-89 -0.871692324806304 1103 90-91 -0.9167628907528567 1104 1 -0.03705310617501567 1104 2 -0.11366421387312187 1104 3 -0.186491863780482 1104 4 -0.07924563081969183 1104 5 -0.09121491474016352 1104 6 -0.04036084000193796 1104 7 -0.03907553788690166 1104 8 -0.07185472347137534 1104 9 -0.1778834967983869 1104 10-11 -0.04150846166366051 1104 12-13 -0.11031842902831102 1104 14-15 -0.14979185151646135 1104 16-17 -0.0870938151216123 1104 18-19 -0.08550276951896052 1104 20-21 -0.07750506298422977 1104 22-23 -0.024307707850255156 1104 24-25 -0.07502339462024565 1104 26-27 -0.06825915884488865 1104 28-29 -0.10058010581887089 1104 30-31 -0.09853883235162897 1104 32-33 -0.1418075963948482 1104 34-35 -0.10758145302119715 1104 36-37 -0.1912661078224005 1104 38-39 -0.12043438717753219 1104 40-41 -0.12483136287145413 1104 42-43 -0.1027486323005391 1104 44-45 -0.19884666946307306 1104 46-47 -0.14888248312680474 1104 48-49 -0.17177397023662166 1104 50-51 -0.15324069329297174 1104 52-53 -0.14817537732673003 1104 54-55 -0.25648200287437106 1104 56-57 -0.23002914793182327 1104 58-59 -0.24020010043025053 1104 60-61 -0.2870834016656616 1104 62-63 -0.2934420675754623 1104 64-65 -0.14268492566681346 1104 66-67 -0.33359561239345226 1104 68-69 -0.14331315343270035 1104 70-71 -0.27346406800297274 1104 72-73 -0.18917727063051615 1104 74-75 -0.27559186301957794 1104 76-77 -0.33908156532753253 1104 78-79 -0.43839819887371334 1104 80-81 -0.23182961208727448 1104 82-83 -0.28176167887018266 1104 84-85 -0.4343138241156126 1104 86-87 -0.8659064253791726 1104 88-89 -0.5465610339981879 1104 90-91 -0.32196776339959143 1201 1 0.07094564187944741 1201 2 0.16429454319333558 1201 3 0.10352145624795384 1201 4 0.06322469027769273 1201 5 -0.1392350030388272 1201 6 0.015666909848064847 1201 7 0.03737850888413874 1201 8 -0.08446302414844808 1201 9 0.022144643970740674 1201 10-11 0.0046497390962869645 1201 12-13 -0.010889964765880222 1201 14-15 -0.10472548169596507 1201 16-17 0.02727146809244374 1201 18-19 -0.016748516173045402 1201 20-21 -0.029639488174254325 1201 22-23 -0.07028821809821295 1201 24-25 -0.025556866925583677 1201 26-27 0.003704146519247331 1201 28-29 0.014118733388890803 1201 30-31 0.034895153643461185 1201 32-33 0.012997322799336075 1201 34-35 0.05498088294324077 1201 36-37 0.034835581652671976 1201 38-39 0.10613018253942386 1201 40-41 -0.019355328342896883 1201 42-43 0.052881461397653595 1201 44-45 -0.015888094827154475 1201 46-47 -0.025441757640798812 1201 48-49 0.04681418243303881 1201 50-51 0.054230920856639386 1201 52-53 -0.0105974249864218 1201 54-55 0.12361094233994407 1201 56-57 0.026583236971681856 1201 58-59 0.0534716337310428 1201 60-61 0.0993524048664014 1201 62-63 -0.13483719288322504 1201 64-65 -0.16277232963673782 1201 66-67 -0.0972266012986509 1201 68-69 -0.26247614150751986 1201 70-71 -0.12434756326344854 1201 72-73 -0.2947229013381829 1201 74-75 -0.05065722087994118 1201 76-77 -0.10077215390261074 1201 78-79 -0.30115397972518565 1201 80-81 -0.10823390855001236 1201 82-83 0.004958446087528046 1201 84-85 -0.1235064143514748 1201 86-87 -0.16828475250352426 1201 88-89 0.25027674153824364 1201 90-91 0.5064929232006321 1202 1 -0.02211771593459133 1202 2 0.027156399340817927 1202 3 -0.1065138511690975 1202 4 -0.03422381741129499 1202 5 -0.05216651511232584 1202 6 -0.01583473197293017 1202 7 -0.13072256643923197 1202 8 -0.17001594273220633 1202 9 0.02760208571569933 1202 10-11 -0.10195318109480667 1202 12-13 -0.10635965983585294 1202 14-15 -0.06939480173853951 1202 16-17 -0.1779339465968448 1202 18-19 -0.0414227690513016 1202 20-21 -0.11903316793503649 1202 22-23 -0.09385024749941806 1202 24-25 -0.05881623214674647 1202 26-27 -0.04683333199576367 1202 28-29 -0.06617841638275479 1202 30-31 -0.025326655443336676 1202 32-33 -0.051980557731710064 1202 34-35 -0.025953180193049263 1202 36-37 -0.22840117584288322 1202 38-39 -0.12387044368505684 1202 40-41 -0.16142599838523353 1202 42-43 -0.20122661032554845 1202 44-45 -0.167743825474993 1202 46-47 -0.11415994329071566 1202 48-49 -0.223584442209102 1202 50-51 -0.13459981673084087 1202 52-53 -0.1815257157647494 1202 54-55 -0.2333414527439146 1202 56-57 -0.11344394359741017 1202 58-59 -0.23076647430634267 1202 60-61 -0.1554220728853366 1202 62-63 -0.49420690357759867 1202 64-65 -0.4135131482069667 1202 66-67 -0.33945272581423325 1202 68-69 -0.32317484091576887 1202 70-71 -0.4370592045188815 1202 72-73 -0.37573945609624104 1202 74-75 -0.6302898278141456 1202 76-77 -0.5706935620090618 1202 78-79 -0.5071612010974462 1202 80-81 -0.3791493397102528 1202 82-83 -0.4233612795396269 1202 84-85 -0.1731279598078146 1202 86-87 -0.3581659447751804 1202 88-89 -0.45429495563895017 1202 90-91 -0.31403855417995175 1203 1 0.03748968617119175 1203 2 0.024718256919520343 1203 3 0.002379405729797668 1203 4 -0.1139679640289728 1203 5 -0.13735692357529672 1203 6 -0.02719296451788722 1203 7 0.051581463158264285 1203 8 -0.016040536357088797 1203 9 -0.011709671217587925 1203 10-11 -0.03128252869766612 1203 12-13 -0.0189986083865179 1203 14-15 -0.05810286108344087 1203 16-17 -0.055913896131720264 1203 18-19 -0.07989005392037285 1203 20-21 -0.045230806711195726 1203 22-23 -0.0780747764647316 1203 24-25 -0.09190088205977531 1203 26-27 -0.08021379697506603 1203 28-29 -0.007073366889528643 1203 30-31 -0.07411215637434054 1203 32-33 -0.0017580687768798953 1203 34-35 1.1743837077204944E-4 1203 36-37 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-0.3071073485820577 1204 6 -0.25083430615500646 1204 7 -0.17807858536771448 1204 8 -0.12179815696642748 1204 9 -0.19312958028981342 1204 10-11 -0.19863332091505725 1204 12-13 -0.22319840021260262 1204 14-15 -0.17490076999626325 1204 16-17 -0.026537944473133734 1204 18-19 -0.061433045668323416 1204 20-21 -0.07465170092420692 1204 22-23 -0.11218257987041369 1204 24-25 -0.1278492328777645 1204 26-27 -0.11911727055513666 1204 28-29 -0.07419297460693741 1204 30-31 -0.06282348555338046 1204 32-33 -0.11378078865912755 1204 34-35 -0.07817113356788497 1204 36-37 -0.06732147942344113 1204 38-39 -0.041904433854902834 1204 40-41 -0.08876995394779641 1204 42-43 -0.11505597321818328 1204 44-45 -0.06571042445740005 1204 46-47 -0.10993611965650274 1204 48-49 -0.07261352386785092 1204 50-51 -0.11550388494660524 1204 52-53 -0.11789143681861702 1204 54-55 -0.10529133964572424 1204 56-57 -0.11133118203623837 1204 58-59 -0.14206662737648657 1204 60-61 -0.15157571847241513 1204 62-63 -0.2060100564980445 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0.08235734183371335 2401 32-33 0.04020842432767324 2401 34-35 6.650026193142367E-4 2401 36-37 0.09664532752738353 2401 38-39 0.16531843822181003 2401 40-41 0.06270882680188805 2401 42-43 0.1427727402732799 2401 44-45 0.13604777378485977 2401 46-47 0.1146439143216682 2401 48-49 0.08315334103234306 2401 50-51 0.10433247276277768 2401 52-53 0.10508045786422571 2401 54-55 0.12981469432216386 2401 56-57 0.14004212424897133 2401 58-59 0.1036001030446343 2401 60-61 0.14387037821890658 2401 62-63 0.2227879446009311 2401 64-65 0.2593595689596526 2401 66-67 0.23919056393734905 2401 68-69 0.21023882445742004 2401 70-71 0.23878749221878337 2401 72-73 0.25556309250410436 2401 74-75 0.26095758342372477 2401 76-77 0.3438544049437482 2401 78-79 0.15730859665650598 2401 80-81 0.201520055278273 2401 82-83 0.19896947609363025 2401 84-85 0.10879896538564537 2401 86-87 0.31085706714893035 2401 88-89 0.07807005339090978 2401 90-91 0.24146208383350398 2402 1 0.022356731462480184 2402 2 0.06172889454550301 2402 3 0.12156495793620081 2402 4 0.15258136278219325 2402 5 0.1472667201742368 2402 6 0.08153888360565986 2402 7 0.03104606305179658 2402 8 0.09692359748530066 2402 9 0.07573582023212566 2402 10-11 0.040125667556019096 2402 12-13 0.09989266878793757 2402 14-15 0.08459554379319201 2402 16-17 0.0841332572778768 2402 18-19 0.06801646378574588 2402 20-21 0.03907690780593498 2402 22-23 0.04440193659421254 2402 24-25 0.051641481399038014 2402 26-27 0.10510312821174495 2402 28-29 0.08754577250869744 2402 30-31 0.08664529645316321 2402 32-33 0.05024170033760811 2402 34-35 0.08379687995819296 2402 36-37 0.11242065618049679 2402 38-39 -0.6560449955302801 2402 40-41 0.07998946495234094 2402 42-43 0.09231870398024711 2402 44-45 0.0875031364298593 2402 46-47 0.13213987427739227 2402 48-49 0.10386812474285989 2402 50-51 0.10507657568321349 2402 52-53 0.14667991790111756 2402 54-55 0.13615570008263234 2402 56-57 0.14036238472375118 2402 58-59 0.11320331619516111 2402 60-61 0.11902587254508035 2402 62-63 0.23614765313768515 2402 64-65 0.143363971050114 2402 66-67 0.1635777231976121 2402 68-69 0.235500126385638 2402 70-71 0.24876108423030985 2402 72-73 0.2968713160489429 2402 74-75 0.25895488428116664 2402 76-77 0.18793342227586152 2402 78-79 0.1637180379667882 2402 80-81 0.24434099392665587 2402 82-83 0.1260048520642698 2402 84-85 0.20229948243833462 2402 86-87 0.13186451680041955 2402 88-89 0.2806857618691936 2402 90-91 0.16272808588740162 2403 1 0.004078868547338743 2403 2 0.12345883153399484 2403 3 0.19497207147288975 2403 4 0.1756972546609319 2403 5 0.12200880697848504 2403 6 0.040555942848818916 2403 7 0.12583195682334747 2403 8 0.08215115201176104 2403 9 0.10703376119271724 2403 10-11 0.056753806826108644 2403 12-13 0.10136024451277592 2403 14-15 0.10649247462291811 2403 16-17 0.05317913459501966 2403 18-19 0.07809023010986493 2403 20-21 0.06371086966620254 2403 22-23 0.05205701266460494 2403 24-25 0.04141529374988551 2403 26-27 0.06918282115206154 2403 28-29 0.07235582366096338 2403 30-31 0.05548646640184529 2403 32-33 0.08919341267353076 2403 34-35 0.06595116426733938 2403 36-37 0.06550472683957054 2403 38-39 0.1391029965739108 2403 40-41 0.04632768632954054 2403 42-43 0.06754324350437457 2403 44-45 0.11330532684484496 2403 46-47 0.05696377054844959 2403 48-49 0.1076473752626157 2403 50-51 0.12783136545431972 2403 52-53 0.11451038721241247 2403 54-55 0.1376638279864082 2403 56-57 0.11413162863474469 2403 58-59 0.11276138897298438 2403 60-61 0.1359074570164438 2403 62-63 0.2083873719407947 2403 64-65 0.15548625597963195 2403 66-67 0.20676666589459813 2403 68-69 0.21120015835337824 2403 70-71 0.207451119394797 2403 72-73 0.2286860173279308 2403 74-75 0.20185625568765886 2403 76-77 0.2530364268179284 2403 78-79 0.18205330548295962 2403 80-81 0.2713490158220324 2403 82-83 0.17956968795749617 2403 84-85 0.1305888506524795 2403 86-87 0.16660989799083836 2403 88-89 0.17313157824120395 2403 90-91 0.5081058264264371 2404 1 0.08240737408419108 2404 2 0.05417308885903083 2404 3 0.09570586748016296 2404 4 0.11444898016780058 2404 5 0.09308785232678218 2404 6 0.12165991280645727 2404 7 0.08618459941176582 2404 8 0.09615140814582901 2404 9 0.12541734530439896 2404 10-11 0.08261180577667915 2404 12-13 0.1390543789106644 2404 14-15 0.12310964555043569 2404 16-17 0.09845897540419912 2404 18-19 0.08240915178198804 2404 20-21 0.08911230361552924 2404 22-23 0.0829982921798873 2404 24-25 0.09504971633359105 2404 26-27 0.06941045127359047 2404 28-29 0.07857607911713416 2404 30-31 0.11174239193967139 2404 32-33 0.03586756825592374 2404 34-35 0.09526385940578308 2404 36-37 0.10923922738498248 2404 38-39 0.14532089317287245 2404 40-41 0.09297719048102948 2404 42-43 0.09665911348789535 2404 44-45 0.15646455024847938 2404 46-47 0.13221418683654207 2404 48-49 0.12713443724716456 2404 50-51 0.09662580879826521 2404 52-53 0.06110954471211727 2404 54-55 0.17588735049068305 2404 56-57 0.15601094106992264 2404 58-59 0.1023486797454396 2404 60-61 0.11141815378859832 2404 62-63 0.2679753364048665 2404 64-65 0.28784443740298826 2404 66-67 0.3193852101662813 2404 68-69 0.2757324328919779 2404 70-71 0.26127935538836056 2404 72-73 0.21133330530186356 2404 74-75 0.2572387814296704 2404 76-77 0.33164174216476283 2404 78-79 0.31469435204978424 2404 80-81 0.24956609917032324 2404 82-83 0.18467456679854877 2404 84-85 0.203273567841606 2404 86-87 0.26592656055552766 2404 88-89 0.23719177278593406 2404 90-91 0.3858946497615605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 80.0 31 1895.0 32 11453.0 33 18933.0 34 23706.0 35 28059.0 36 37819.0 37 53037.0 38 122412.0 39 810526.0 40 811173.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.196139530496957 11.313886299413317 25.277774448658818 33.212199721430906 2 30.78147758515888 15.841204271836967 24.121667905650916 29.255650237353237 3 28.527322845723756 17.779937366147497 18.667389231354445 35.025350556774306 4 28.306392655280384 19.262641258136004 17.980056203633694 34.450909882949915 5 28.792247170929187 19.753862892522665 18.063793677534125 33.39009625901402 6 28.837320546737445 22.623082883424615 20.847243984528106 27.69235258530983 7 27.20316316093071 21.468891815039708 20.806912432070774 30.521032591958807 8 26.7509703802786 22.405636412617834 20.85016202966714 29.993231177436424 9 26.785048978866577 22.339771965193975 21.665286674486335 29.209892381453113 10-11 27.062549860793617 20.826817668554884 21.09655446609414 31.014078004557362 12-13 27.563281195856586 20.724946628433326 20.960995637001435 30.750776538708653 14-15 28.092958496539772 20.4560175041022 20.650458315464647 30.80056568389338 16-17 28.30717427451405 20.221427518103603 20.181174127569637 31.290224079812702 18-19 28.408628451044322 20.592123466658467 20.192325228636655 30.806922853660556 20-21 28.32106104289891 20.679247957238132 20.217545475909713 30.78214552395324 22-23 28.333957760254453 20.13375589406037 20.245345066653883 31.286941279031293 24-25 28.459042891615987 20.492831770008017 20.303784131357887 30.744341207018106 26-27 28.663879238786237 20.641417586328544 20.276010594588172 30.418692580297048 28-29 28.58759320158012 20.327389032214697 20.315742905633023 30.769274860572153 30-31 28.519714978975674 20.5714541788502 20.53344130136917 30.37538954080496 32-33 28.641636955543447 20.605458766792538 20.56714959756929 30.185754680094725 34-35 28.629747463637877 20.24215484819383 20.59247058571053 30.535627102457763 36-37 28.656764225720366 20.60233526334308 20.690174337113916 30.05072617382264 38-39 29.094721019565085 20.586213837357402 20.537950935347446 29.78111420773007 40-41 28.72970940647383 20.343492057961925 20.72162075062494 30.20517778493931 42-43 28.737941046351917 20.593596608019382 20.889594916600476 29.778867429028228 44-45 28.913173260380148 20.69286764381657 20.80110280401305 29.59285629179023 46-47 28.81849469328922 20.33849620781364 20.85950566445189 29.983503434445247 48-49 28.914840098152762 20.655827084771342 20.918653197455473 29.51067961962043 50-51 29.055793852409018 20.74632919081874 20.82452990502554 29.373347051746705 52-53 29.045572457625347 20.45133964717484 20.833623316717762 29.669464578482053 54-55 28.999441872177833 20.713160637904185 21.030441978314936 29.256955511603046 56-57 29.10349291600119 20.81389911340415 20.953112025348013 29.129495945246646 58-59 29.09713724650166 20.497063147162574 20.864480031331333 29.541319575004437 60-61 29.42410166537912 20.72442707520385 20.88811987410374 28.963351385313292 62-63 29.382686781379046 20.82031846514792 20.85897596080429 28.938018792668746 64-65 29.304679502477338 20.566561743524176 20.827214752182222 29.301544001816264 66-67 29.315925285799494 20.832617960251966 20.897414864244446 28.954041889704097 68-69 29.3877115954926 20.850330046542418 20.838619056935745 28.923339301029234 70-71 29.471916483821627 20.684980550695723 20.69941055387032 29.14369241161233 72-73 29.36478775543491 20.94191390605941 20.864920608334746 28.82837773017094 74-75 29.547024091282793 21.03799297441391 20.540123072323674 28.87485986197962 76-77 29.525696150460885 20.715144216958564 20.518542180386678 29.240617452193877 78-79 29.54792951149507 21.05303640610641 20.581817592978677 28.817216489419845 80-81 29.67275230281208 21.073043867880116 20.360690015682177 28.89351381362563 82-83 29.996214683805288 20.591918752617506 20.08653877130247 29.325327792274734 84-85 30.273259528472824 20.712056306754505 19.691391644060875 29.323292520711796 86-87 30.776961107932454 19.864515939732698 18.896982233763186 30.461540718571662 88-89 29.97036853031459 19.612957409937483 19.267552653524724 31.149121406223202 90-91 25.97147975231674 22.394459568105844 21.998109934129793 29.63595074544762 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 3.5 10 6.0 11 6.0 12 11.0 13 22.5 14 30.0 15 36.0 16 48.0 17 59.0 18 89.0 19 141.0 20 207.5 21 297.5 22 408.0 23 526.5 24 692.5 25 908.0 26 1165.0 27 1483.5 28 1887.0 29 2363.5 30 2879.5 31 3512.5 32 4295.0 33 5107.0 34 6145.0 35 7594.0 36 9185.0 37 10901.0 38 12964.5 39 15491.0 40 18593.5 41 22121.0 42 25838.5 43 30005.0 44 34653.0 45 39753.5 46 45564.5 47 52035.0 48 58879.0 49 66478.5 50 74173.5 51 82304.0 52 90228.5 53 97331.0 54 105521.0 55 113764.0 56 120854.0 57 127461.0 58 133691.0 59 139560.5 60 144619.5 61 148023.5 62 149509.0 63 148914.0 64 145761.0 65 140121.5 66 131547.5 67 121750.5 68 111094.5 69 98544.5 70 85735.0 71 73568.0 72 61857.0 73 50589.5 74 40601.5 75 32474.5 76 25647.5 77 19738.5 78 15291.0 79 11766.0 80 8811.0 81 6514.0 82 4873.0 83 3640.5 84 2699.0 85 2007.0 86 1468.5 87 1034.0 88 733.5 89 516.0 90 353.5 91 248.0 92 173.5 93 118.0 94 76.5 95 50.0 96 35.0 97 25.0 98 19.0 99 10.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.044552296319146596 3 1.563238467338477E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.13957563152483704 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 5.3394302507556626E-5 64-65 2.3225876878465375E-4 66-67 1.2717727494709425E-4 68-69 1.4025117583079535E-4 70-71 1.559997909602801E-4 72-73 1.7538305852269587E-4 74-75 1.993070095278716E-4 76-77 4.5961690930609335E-4 78-79 1.3494220425391804E-4 80-81 0.0011363765497335197 82-83 0.0 84-85 5.236835903746956E-4 86-87 3.6676654942362636E-4 88-89 0.0 90-91 0.0028209142583111185 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 13783.0 32-33 32174.0 34-35 38853.0 36-37 45524.0 38-39 52311.0 40-41 58650.0 42-43 62443.0 44-45 66555.0 46-47 69108.0 48-49 71956.0 50-51 74072.0 52-53 75843.0 54-55 75545.0 56-57 75691.0 58-59 75524.0 60-61 75741.0 62-63 75492.0 64-65 75201.0 66-67 73409.0 68-69 72445.0 70-71 71253.0 72-73 68777.0 74-75 67256.0 76-77 65049.0 78-79 63075.0 80-81 60465.0 82-83 57927.0 84-85 55245.0 86-87 52543.0 88-89 49626.0 90-91 47557.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.93925947003301 #Duplication Level Percentage of deduplicated Percentage of total 1 99.05708809528106 98.00634941404931 2 0.8633882797814972 1.7084599407337402 3 0.05678035963309551 0.16853420203621877 4 0.012838402249140506 0.05080888045233472 5 0.003363290410386591 0.016638073129315634 6 0.0024917338544206856 0.014791818141167618 7 0.001684901608824267 0.011669204221985814 8 0.0010581334934072876 0.008375275540652488 9 5.509430691471396E-4 0.004905890934440867 >10 7.558606190259213E-4 0.009467300760795721 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0011463748760482165 0.0 0.0 0.0 5.210794891128257E-5 2 0.001198482824959499 0.0 0.0 0.0 5.210794891128257E-5 3 0.001198482824959499 0.0 5.210794891128257E-5 0.0 1.0421589782256514E-4 4 0.001198482824959499 0.0 5.210794891128257E-5 0.0 1.0421589782256514E-4 5 0.0012505907738707817 0.0 5.210794891128257E-5 0.0 1.0421589782256514E-4 6 0.0014069146206046293 0.0 5.210794891128257E-5 0.0 1.0421589782256514E-4 7 0.0015111305184271945 0.0 5.210794891128257E-5 0.0 2.084317956451303E-4 8 0.004585499504192866 0.0 5.210794891128257E-5 0.0 2.084317956451303E-4 9 0.004898147197660562 0.0 5.210794891128257E-5 0.0 2.084317956451303E-4 10-11 0.004924201172116203 0.0 5.210794891128257E-5 0.0 2.084317956451303E-4 12-13 0.004950255146571844 0.0 5.210794891128257E-5 0.0 2.3448577010077155E-4 14-15 0.004950255146571844 0.0 1.0421589782256514E-4 0.0 2.6053974455641284E-4 16-17 0.004950255146571844 0.0 1.0421589782256514E-4 0.0 2.6053974455641284E-4 18-19 0.004976309121027485 0.0 1.0421589782256514E-4 0.0 2.6053974455641284E-4 20-21 0.005002363095483127 0.0 1.0421589782256514E-4 0.0 4.168635912902606E-4 22-23 0.005002363095483127 0.0 1.0421589782256514E-4 0.0 4.168635912902606E-4 24-25 0.005028417069938769 0.0 1.3026987227820642E-4 0.0 4.168635912902606E-4 26-27 0.005080525018850051 0.0 1.563238467338477E-4 0.0 4.168635912902606E-4 28-29 0.005184740916672616 0.0 1.82377821189489E-4 0.0 5.210794891128257E-4 30-31 0.0052629028400395395 0.0 2.084317956451303E-4 0.0 6.252953869353908E-4 32-33 0.0053671187378621045 0.0 2.3448577010077155E-4 0.0 7.29511284757956E-4 34-35 0.005549496559051594 0.0 3.126476934676954E-4 0.0 8.076732081248798E-4 36-37 0.005653712456874158 0.0 3.64755642378978E-4 0.0 8.858351314918036E-4 38-39 0.005914252201430572 0.0 4.4291756574590187E-4 0.0 9.379430804030862E-4 40-41 0.006200845920442626 0.0 4.689715402015431E-4 0.0 9.379430804030862E-4 42-43 0.0064092777160877565 0.0 4.950255146571843E-4 0.0 9.379430804030862E-4 44-45 0.0065134936139103206 0.0 5.47133463568467E-4 0.0 9.379430804030862E-4 46-47 0.00669587143509981 0.0 5.731874380241083E-4 0.0 9.379430804030862E-4 48-49 0.006826141307378016 0.0 5.731874380241083E-4 0.0 9.90051029314369E-4 50-51 0.006982465154111864 0.0 5.731874380241083E-4 0.0 9.90051029314369E-4 52-53 0.007138789000845712 0.0 5.731874380241083E-4 0.0 9.90051029314369E-4 54-55 0.00729511284757956 0.0 5.731874380241083E-4 0.0 0.0010421589782256514 56-57 0.007451436694313408 0.0 5.731874380241083E-4 0.0 0.0010421589782256514 58-59 0.007477490668769049 0.0 5.731874380241083E-4 0.0 0.0010421589782256514 60-61 0.007790138362236744 0.0 5.731874380241083E-4 0.0 0.001094266927136934 62-63 0.007868300285603667 0.0 5.731874380241083E-4 0.0 0.001094266927136934 64-65 0.007920408234514951 0.0 5.731874380241083E-4 0.0 0.001094266927136934 66-67 0.007998570157881874 0.0 5.731874380241083E-4 0.0 0.001094266927136934 68-69 0.008207001953527004 0.0 5.992414124797495E-4 0.0 0.001094266927136934 70-71 0.008233055927982645 0.0 6.252953869353908E-4 0.0 0.001094266927136934 72-73 0.008337271825805211 0.0 6.252953869353908E-4 0.0 0.001094266927136934 74-75 0.008389379774716493 0.0 6.252953869353908E-4 0.0 0.001094266927136934 76-77 0.008441487723627777 0.0 6.252953869353908E-4 0.0 0.001094266927136934 78-79 0.0085196496469947 0.0 6.252953869353908E-4 0.0 0.001094266927136934 80 0.008702027468184189 0.0 6.252953869353908E-4 0.0 0.001094266927136934 >>END_MODULE