##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-33C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 577469 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 57 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.576967767966764 40.0 40.0 40.0 40.0 40.0 2 39.351175560939204 40.0 40.0 40.0 40.0 40.0 3 39.31688107933066 40.0 40.0 40.0 40.0 40.0 4 39.21462104459287 40.0 40.0 40.0 40.0 40.0 5 39.2657614521299 40.0 40.0 40.0 40.0 40.0 6 39.36671925246204 40.0 40.0 40.0 40.0 40.0 7 39.370238056068814 40.0 40.0 40.0 40.0 40.0 8 39.40077822359296 40.0 40.0 40.0 40.0 40.0 9 39.395874064235485 40.0 40.0 40.0 40.0 40.0 10-11 39.40087173510612 40.0 40.0 40.0 40.0 40.0 12-13 39.31621610857033 40.0 40.0 40.0 40.0 40.0 14-15 39.348723481260464 40.0 40.0 40.0 40.0 40.0 16-17 39.457193373150766 40.0 40.0 40.0 40.0 40.0 18-19 39.48671184080877 40.0 40.0 40.0 40.0 40.0 20-21 39.4684770957402 40.0 40.0 40.0 40.0 40.0 22-23 39.500651983050176 40.0 40.0 40.0 40.0 40.0 24-25 39.470537812419366 40.0 40.0 40.0 40.0 40.0 26-27 39.43921145550671 40.0 40.0 40.0 40.0 40.0 28-29 39.45366764276524 40.0 40.0 40.0 40.0 40.0 30-31 39.43249390363413 40.0 40.0 40.0 40.0 40.0 32-33 39.4354105616456 40.0 40.0 40.0 40.0 40.0 34-35 39.424045859272155 40.0 40.0 40.0 40.0 40.0 36-37 39.402763908014826 40.0 40.0 40.0 40.0 40.0 38-39 39.34039630464119 40.0 40.0 40.0 40.0 40.0 40-41 39.39074464473172 40.0 40.0 40.0 40.0 40.0 42-43 39.3646598148749 40.0 40.0 40.0 40.0 40.0 44-45 39.26466389498303 40.0 40.0 40.0 40.0 40.0 46-47 39.2338551403936 40.0 40.0 40.0 40.0 40.0 48-49 39.31278843124383 40.0 40.0 40.0 40.0 40.0 50-51 39.26535547655823 40.0 40.0 40.0 40.0 40.0 52-53 39.21917569667201 40.0 40.0 40.0 40.0 40.0 54-55 39.13387574322027 40.0 40.0 40.0 40.0 40.0 56-57 39.12548723809665 40.0 40.0 40.0 40.0 40.0 58-59 39.08113875719605 40.0 40.0 40.0 40.0 40.0 60-61 38.9855039839245 40.0 40.0 40.0 40.0 40.0 62-63 39.38725370809369 40.0 40.0 40.0 40.0 40.0 64-65 39.385442253414595 40.0 40.0 40.0 40.0 40.0 66-67 39.382472623441274 40.0 40.0 40.0 40.0 40.0 68-69 39.401828326609305 40.0 40.0 40.0 40.0 40.0 70-71 39.384017963190146 40.0 40.0 40.0 40.0 40.0 72-73 39.39302016194553 40.0 40.0 40.0 40.0 40.0 74-75 39.38203486493139 40.0 40.0 40.0 40.0 40.0 76-77 39.386545179592474 40.0 40.0 40.0 40.0 40.0 78-79 39.395492240348744 40.0 40.0 40.0 40.0 40.0 80-81 39.400705485760966 40.0 40.0 40.0 40.0 40.0 82-83 39.38159113421378 40.0 40.0 40.0 40.0 40.0 84-85 39.40841334701243 40.0 40.0 40.0 40.0 40.0 86-87 39.34165598484153 40.0 40.0 40.0 40.0 40.0 88-89 39.391701117393495 40.0 40.0 40.0 40.0 40.0 90-91 39.498067596406194 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.016665329716367694 1101 2 -0.06837654616224853 1101 3 0.011308250941446829 1101 4 0.07037715450189097 1101 5 0.14620551982760333 1101 6 -0.07475187341684375 1101 7 -0.08441493237963016 1101 8 0.08894184104209302 1101 9 0.0956008166757556 1101 10-11 0.034738636653557364 1101 12-13 0.040721296818809094 1101 14-15 0.1185443787876892 1101 16-17 0.05566090633204368 1101 18-19 0.059522354970503955 1101 20-21 -0.014428727925718476 1101 22-23 -0.05955633664509463 1101 24-25 0.07715358745116419 1101 26-27 -0.015749950162735615 1101 28-29 0.044654585264844115 1101 30-31 0.07995117151860853 1101 32-33 0.08344800396400132 1101 34-35 0.03822187183192938 1101 36-37 0.0015354779044898237 1101 38-39 0.09557807098357785 1101 40-41 -0.16844381358342986 1101 42-43 0.1433317124125466 1101 44-45 -0.025724868472444484 1101 46-47 0.06596411619706544 1101 48-49 -0.030431859328018618 1101 50-51 0.04066505220940542 1101 52-53 0.014890893883489298 1101 54-55 0.022876041041627104 1101 56-57 0.13457204537193945 1101 58-59 0.368208616820894 1101 60-61 0.03950061417931039 1101 62-63 -0.02130477735033054 1101 64-65 0.09166306306079974 1101 66-67 -0.04207770738460681 1101 68-69 -0.07393381939915145 1101 70-71 0.05399501784130223 1101 72-73 -0.24189611783409504 1101 74-75 0.058488546282866594 1101 76-77 0.0220707934702844 1101 78-79 -0.1858688057090987 1101 80-81 -0.35589954470476926 1101 82-83 -0.3718560105729196 1101 84-85 0.39072439329780195 1101 86-87 0.21996657209584214 1101 88-89 0.04344731247188349 1101 90-91 0.7757783882783897 1102 1 0.03399727367244054 1102 2 -0.05343763457435102 1102 3 -0.08844309873252598 1102 4 -0.06297103154425088 1102 5 1.5030752000910752E-4 1102 6 -0.11610182587489959 1102 7 -0.06535837139266931 1102 8 -0.13344782783524067 1102 9 0.06759602667587217 1102 10-11 0.017936240062255138 1102 12-13 -0.0178551502266302 1102 14-15 -0.015906621184470282 1102 16-17 -0.05596757427522192 1102 18-19 -0.07572671308807344 1102 20-21 -0.07120614013226145 1102 22-23 0.03607099818147219 1102 24-25 -0.11893723158311076 1102 26-27 -0.11323360886331102 1102 28-29 -0.15806823526531133 1102 30-31 -0.03304985888834011 1102 32-33 -0.08812265712821699 1102 34-35 0.003026141536366822 1102 36-37 -0.028409087554265966 1102 38-39 -0.043985657730580385 1102 40-41 -0.06875097011563724 1102 42-43 -0.1650908018273043 1102 44-45 -0.1577043635932185 1102 46-47 -0.06492696480454185 1102 48-49 -0.052597011633139346 1102 50-51 0.044033724287388054 1102 52-53 0.01888345560023197 1102 54-55 -0.16704759052008455 1102 56-57 -0.27307552077638064 1102 58-59 -0.22074941248168045 1102 60-61 -0.015538027649455444 1102 62-63 -0.25443382732209585 1102 64-65 -0.022831961570162207 1102 66-67 -0.09264328477952688 1102 68-69 -0.10760204888929792 1102 70-71 -0.04107025077770743 1102 72-73 0.15338454268037793 1102 74-75 0.1527030187813665 1102 76-77 0.2944388919032903 1102 78-79 0.09381434756382845 1102 80-81 -0.06313411950550574 1102 82-83 0.07771735836137594 1102 84-85 0.536178938752343 1102 86-87 0.38081313294240005 1102 88-89 0.5140355477659995 1102 90-91 0.7757783882783897 1103 1 0.0887646065893648 1103 2 0.09112982326450236 1103 3 0.0235853210051431 1103 4 -0.018508195816579587 1103 5 0.029692780974102106 1103 6 -0.024067160041049362 1103 7 0.11169971730189587 1103 8 -0.0014084774292442148 1103 9 0.007272791198047912 1103 10-11 -0.01991900665854729 1103 12-13 0.006700596181865137 1103 14-15 -0.048828232677280425 1103 16-17 -0.12009546309470664 1103 18-19 -0.16858274057089773 1103 20-21 -0.009086371237820856 1103 22-23 -0.03171397995719616 1103 24-25 -0.06309322783545923 1103 26-27 -0.10990600111814786 1103 28-29 -0.039756242760624616 1103 30-31 -0.028424624659734832 1103 32-33 -0.0627663830127716 1103 34-35 -0.11418372594246051 1103 36-37 -0.02969312864559015 1103 38-39 0.036389218055532524 1103 40-41 -0.026435591985922713 1103 42-43 0.12584727549489116 1103 44-45 -0.0284263296783962 1103 46-47 0.08757601844661167 1103 48-49 0.03253331622299527 1103 50-51 0.0022848390244192274 1103 52-53 0.13241765884671963 1103 54-55 0.04013683785035482 1103 56-57 0.05337843269113307 1103 58-59 0.07341961339466252 1103 60-61 0.09029175788794674 1103 62-63 0.18235552590302717 1103 64-65 0.07474145000190191 1103 66-67 0.30466368344831807 1103 68-69 -0.05555989429754504 1103 70-71 -0.4154446565641692 1103 72-73 -0.2641367577162157 1103 74-75 -0.23650505442691383 1103 76-77 0.019476660723306338 1103 78-79 0.018322600308700032 1103 80-81 0.209397036986104 1103 82-83 -0.29596294752231955 1103 84-85 -0.2286284217996979 1103 86-87 -0.2906325062451742 1103 88-89 0.5140355477659995 1103 90-91 -1.2242216117216103 1104 1 0.013102213426975595 1104 2 0.052549237183917796 1104 3 0.004302659222481964 1104 4 -0.06309305540143839 1104 5 -0.09564604686472222 1104 6 0.02037423190034815 1104 7 0.014105089707271645 1104 8 0.1371690561482879 1104 9 0.04264824907350828 1104 10-11 0.003913410923878757 1104 12-13 -0.07261716938590013 1104 14-15 -0.12643201778107027 1104 16-17 0.0248450130957778 1104 18-19 -0.026229260717414604 1104 20-21 -0.08310499150643835 1104 22-23 -0.052530524523739075 1104 24-25 -0.09937985787208703 1104 26-27 -0.023486587198732423 1104 28-29 -6.017861061664576E-4 1104 30-31 -0.02316821440332717 1104 32-33 -0.03621251151065508 1104 34-35 -0.13996685325099634 1104 36-37 -0.14832478692271422 1104 38-39 -0.09675274935861466 1104 40-41 -0.046634633825128446 1104 42-43 -0.06593639534001738 1104 44-45 -0.10314281733890596 1104 46-47 -0.05782989663030946 1104 48-49 -0.0941442794499423 1104 50-51 -0.2605031255306045 1104 52-53 -0.06742270933709449 1104 54-55 0.07056151289329193 1104 56-57 -0.11554801672509996 1104 58-59 -0.09174328905297102 1104 60-61 -0.05207344928197699 1104 62-63 -0.05374867896684776 1104 64-65 -0.2660597495665584 1104 66-67 -0.13172958910172383 1104 68-69 -0.2024585276810953 1104 70-71 -0.14799857060054222 1104 72-73 -0.12879575555560763 1104 74-75 -0.07118670842142194 1104 76-77 -0.030105151101643912 1104 78-79 0.1450226329493276 1104 80-81 -0.19010090645691946 1104 82-83 -0.6909548168440409 1104 84-85 -0.41766721509381455 1104 86-87 -0.21799546586619556 1104 88-89 0.20634324007369287 1104 90-91 0.7757783882783897 1201 1 0.10888496003239112 1201 2 -0.07435006704628222 1201 3 0.01282511381440088 1201 4 -0.2102114255545473 1201 5 -0.02831910202407073 1201 6 -0.03701505766444768 1201 7 0.022565043322614997 1201 8 -0.015543151408529354 1201 9 -0.01729911617550073 1201 10-11 0.040322461958147926 1201 12-13 0.02872146760173422 1201 14-15 0.09194416220388746 1201 16-17 -0.04366721812212404 1201 18-19 0.07587947758876368 1201 20-21 0.09475046605651016 1201 22-23 -0.0356909756915087 1201 24-25 -0.0519148305167505 1201 26-27 -0.015102527626375206 1201 28-29 0.0174542995915985 1201 30-31 0.08394283420680893 1201 32-33 -0.03608324315334954 1201 34-35 -0.041882902369380304 1201 36-37 0.006952029367518264 1201 38-39 0.1378116947328465 1201 40-41 0.026475327366604517 1201 42-43 -0.029309978931429725 1201 44-45 0.03068025795334961 1201 46-47 0.15559364652469299 1201 48-49 -0.06925110296423043 1201 50-51 -0.044172346286728725 1201 52-53 0.0738107181598906 1201 54-55 0.07195365996190617 1201 56-57 0.010599147701015 1201 58-59 -0.028833309790648798 1201 60-61 0.04000262635154428 1201 62-63 0.08738446368571573 1201 64-65 0.21740279175553923 1201 66-67 0.15227392652914062 1201 68-69 0.051608953194978824 1201 70-71 0.0449413121789064 1201 72-73 0.0715971996012712 1201 74-75 -0.16800478296714516 1201 76-77 0.3016819161872135 1201 78-79 -0.2188790567588157 1201 80-81 -0.3485981859554954 1201 82-83 0.2369855854262113 1201 84-85 0.18769409026749173 1201 86-87 0.6771094292386977 1201 88-89 0.1503991841296397 1201 90-91 0.7757783882783897 1202 1 -0.02420697744932454 1202 2 -0.04093486839208538 1202 3 0.10743961362908294 1202 4 -0.040267567037865604 1202 5 -0.0295968199932517 1202 6 0.07704227163972632 1202 7 -0.08381418838129662 1202 8 -0.06871803002900378 1202 9 -0.11172055825781513 1202 10-11 -0.02068230774681723 1202 12-13 -0.0035427532739973344 1202 14-15 0.10486281931787289 1202 16-17 -0.029338207955646567 1202 18-19 0.0320067394774739 1202 20-21 0.013584426827470963 1202 22-23 0.005941703235066598 1202 24-25 0.11590465608231426 1202 26-27 -0.09641446544704735 1202 28-29 -0.014692760293876006 1202 30-31 -0.1021066077310806 1202 32-33 -9.427828565335972E-4 1202 34-35 0.0016984879141830334 1202 36-37 -0.11470627841572423 1202 38-39 -0.15267143140105333 1202 40-41 -0.005958897724276824 1202 42-43 -0.07218674617154619 1202 44-45 -0.14910285750583796 1202 46-47 -0.06136740438279986 1202 48-49 -0.1764332050686903 1202 50-51 -0.048825623127164874 1202 52-53 -0.13255951334032545 1202 54-55 -0.0372454350539968 1202 56-57 -0.06948606244779398 1202 58-59 0.005390921316219988 1202 60-61 -0.11122364921957484 1202 62-63 -0.341483550388773 1202 64-65 -0.44952989185940595 1202 66-67 -0.23964658684301554 1202 68-69 -0.13414389045259867 1202 70-71 0.002706785419277935 1202 72-73 0.28164182273226857 1202 74-75 0.0953289372806907 1202 76-77 0.1146444082820679 1202 78-79 -0.36288434379485324 1202 80-81 0.534166825163851 1202 82-83 0.42619193463256266 1202 84-85 -0.6009079645524338 1202 86-87 0.21996657209584214 1202 88-89 0.5140355477659995 1202 90-91 0.7757783882783897 1203 1 0.011291536571413019 1203 2 0.08327374905744023 1203 3 0.04130973752219802 1203 4 -0.09638671167535051 1203 5 -0.07450979233529154 1203 6 -0.01309917679748196 1203 7 0.08634364309483544 1203 8 0.005147477089003871 1203 9 -0.22432355829327122 1203 10-11 0.004643381133199398 1203 12-13 -0.02996981076008609 1203 14-15 -0.19231566535232503 1203 16-17 -0.02704863484814979 1203 18-19 -0.059045038596032384 1203 20-21 0.010532868738245327 1203 22-23 -0.011853866252828027 1203 24-25 -0.0474401757708236 1203 26-27 0.021600581766350047 1203 28-29 -0.024581197877324712 1203 30-31 0.0040379725874259975 1203 32-33 -0.026515338486170492 1203 34-35 0.05443755116542803 1203 36-37 -0.023391888533282668 1203 38-39 0.04817514919410826 1203 40-41 0.0075465023352379035 1203 42-43 0.04400516211416061 1203 44-45 0.06859390126328435 1203 46-47 0.07493247399333569 1203 48-49 0.08535248994580513 1203 50-51 -0.009793464579438194 1203 52-53 0.024948365588038257 1203 54-55 -0.14362829622589857 1203 56-57 0.057337470716746 1203 58-59 0.1920775315068397 1203 60-61 0.2640152702410532 1203 62-63 -0.10587130690193902 1203 64-65 0.16315071519360203 1203 66-67 -0.07296894738064452 1203 68-69 0.13116437875800813 1203 70-71 -0.03783422514207757 1203 72-73 0.08820891457577318 1203 74-75 0.30434066929223036 1203 76-77 0.06486329508631883 1203 78-79 0.26292107437718215 1203 80-81 0.4479769916056 1203 82-83 -0.5809722444719156 1203 84-85 -0.5636573951265405 1203 86-87 0.2771094292386991 1203 88-89 0.5140355477659995 1203 90-91 0.7757783882783897 1204 1 -0.012736611437432543 1204 2 3.83781851589049E-4 1204 3 -0.18896949020070508 1204 4 -0.02933036390427901 1204 5 -0.3080021033133491 1204 6 -0.029301630199391582 1204 7 -0.1305292717358597 1204 8 -0.07606742992620497 1204 9 -0.08270589333301359 1204 10-11 -0.08480177157937163 1204 12-13 0.03887020641328576 1204 14-15 -0.01855113158106292 1204 16-17 -0.08106531693149321 1204 18-19 -0.055412825832767965 1204 20-21 -0.04018107282125527 1204 22-23 -0.09261684232016876 1204 24-25 -0.10028110846883465 1204 26-27 -0.005006488328866965 1204 28-29 -0.0053683012259142515 1204 30-31 -0.09724922880103293 1204 32-33 -0.13023023840649728 1204 34-35 -0.070295674596494 1204 36-37 -0.12210315022881701 1204 38-39 -0.07343774567645767 1204 40-41 -0.14337143043092482 1204 42-43 -0.12397156181030056 1204 44-45 -0.19537075940162651 1204 46-47 -0.15716284297533178 1204 48-49 -0.028554266988919608 1204 50-51 -0.04716519420166776 1204 52-53 0.011645023365545626 1204 54-55 -0.17585679439832802 1204 56-57 -0.21282496923038963 1204 58-59 -0.27105865608361057 1204 60-61 -0.01342394607001296 1204 62-63 -0.08277513675136561 1204 64-65 -0.2620025997902147 1204 66-67 -0.22088885212436793 1204 68-69 -0.1929709859949824 1204 70-71 -0.07826093458287176 1204 72-73 -0.4986849976529939 1204 74-75 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0.10889479907008592 2401 50-51 0.1412440038761673 2401 52-53 0.0834483704040565 2401 54-55 0.22098959107574956 2401 56-57 0.21406261077081723 2401 58-59 0.13727443310187226 2401 60-61 0.19369361508001504 2401 62-63 0.07474270420529194 2401 64-65 0.004353296759937564 2401 66-67 0.059701509441659084 2401 68-69 0.1338492195228227 2401 70-71 0.21984660047007765 2401 72-73 0.3002271685568516 2401 74-75 0.2921930373770891 2401 76-77 -0.12225867385382827 2401 78-79 0.2013945388357925 2401 80-81 0.4636058072344156 2401 82-83 0.10365225209287843 2401 84-85 -0.011969209395800817 2401 86-87 0.36941712154639106 2401 88-89 0.09298291618705434 2401 90-91 -1.6686660561660531 2402 1 -0.10655590623114364 2402 2 -0.052226490233323375 2402 3 -0.020900648999699456 2402 4 0.13659823862947462 2402 5 0.05488266970172617 2402 6 0.030464770292773835 2402 7 0.02825027366377242 2402 8 -0.01179309281386054 2402 9 0.015832297729254208 2402 10-11 0.06286703591523946 2402 12-13 -0.043382253358529965 2402 14-15 0.06650921289600831 2402 16-17 0.11614365156422224 2402 18-19 0.005286393439753567 2402 20-21 0.036921732293869525 2402 22-23 0.0788768521666583 2402 24-25 -0.006353387487131101 2402 26-27 0.059449344793804926 2402 28-29 0.021214971559636808 2402 30-31 -0.07203130100226218 2402 32-33 -0.014521546945992725 2402 34-35 0.09396129695446831 2402 36-37 0.04213359344653611 2402 38-39 -0.35978700753692294 2402 40-41 0.0291002089279786 2402 42-43 -0.07179515488806487 2402 44-45 0.05030827738861632 2402 46-47 0.032439852718191275 2402 48-49 0.0628647934695934 2402 50-51 0.09993180943253321 2402 52-53 -0.0756085397953683 2402 54-55 -0.1027026611913584 2402 56-57 0.04885040488454706 2402 58-59 0.09181116715278392 2402 60-61 0.002687280215496912 2402 62-63 0.24611076600600512 2402 64-65 0.3081335677764443 2402 66-67 0.10517030864746602 2402 68-69 0.1268584574262448 2402 70-71 0.09180613449828456 2402 72-73 -0.12516985705342876 2402 74-75 0.07828675104514105 2402 76-77 -0.16629055106752588 2402 78-79 0.056322067977582435 2402 80-81 0.30942564089619395 2402 82-83 0.0693745747772212 2402 84-85 -0.657018340159226 2402 86-87 -0.13095508689033863 2402 88-89 -0.33325509262809305 2402 90-91 0.7757783882783897 2403 1 -0.016177637635053088 2403 2 0.04645632019990842 2403 3 0.038079663296514354 2403 4 0.05721787679068058 2403 5 -0.07120184420269737 2403 6 -0.03729087526971142 2403 7 0.03443899122928684 2403 8 -0.018756426509867197 2403 9 0.05671998591751759 2403 10-11 0.08279492544895106 2403 12-13 0.05080066690322127 2403 14-15 0.06084055563012214 2403 16-17 -0.0048579572012599215 2403 18-19 0.11862258710473128 2403 20-21 0.03268460943167639 2403 22-23 0.06205016431437116 2403 24-25 0.0770971304860879 2403 26-27 0.021339873469308657 2403 28-29 0.006204389016836842 2403 30-31 0.05926750169575712 2403 32-33 0.046301106393485725 2403 34-35 -0.005084159002144872 2403 36-37 0.04730948089095932 2403 38-39 0.10651205007225428 2403 40-41 0.003916177763805706 2403 42-43 0.005314541655124572 2403 44-45 0.04442515181982287 2403 46-47 -0.0683726754267866 2403 48-49 0.06341854134436176 2403 50-51 0.16734433167449936 2403 52-53 -0.038607890048851345 2403 54-55 0.17275795537673844 2403 56-57 -0.0574361947457902 2403 58-59 0.13208550131763275 2403 60-61 0.21984277229140758 2403 62-63 0.02540840804361011 2403 64-65 0.09806447254968731 2403 66-67 -0.01832780536794587 2403 68-69 0.04812044385255376 2403 70-71 0.04283283919104974 2403 72-73 0.19761641930424645 2403 74-75 -0.09686554057745411 2403 76-77 -0.1879982695912119 2403 78-79 -0.4851398254574022 2403 80-81 -0.38714737217044615 2403 82-83 -0.3644574160167906 2403 84-85 0.39072439329780195 2403 86-87 0.22198122411049326 2403 88-89 -0.16321312948267774 2403 90-91 0.7757783882783897 2404 1 0.08814695953054041 2404 2 -0.08513488261785085 2404 3 0.14090885041690626 2404 4 0.07555062771282905 2404 5 0.08466336920939455 2404 6 0.05243283995895354 2404 7 0.122170305537189 2404 8 0.05582681668840195 2404 9 0.03700808531569777 2404 10-11 0.03950746392968796 2404 12-13 0.03977412559363103 2404 14-15 0.0647894143815364 2404 16-17 0.015007478081763281 2404 18-19 0.06568196531146242 2404 20-21 -0.017189312414295443 2404 22-23 0.07980072592515342 2404 24-25 0.049715595693953674 2404 26-27 0.11741752829361474 2404 28-29 0.03733199253348829 2404 30-31 -0.024483448189144497 2404 32-33 0.06364853765783351 2404 34-35 0.027267219801643705 2404 36-37 0.03199762711383869 2404 38-39 0.0893633556228366 2404 40-41 0.0986145609789375 2404 42-43 0.14240992643374284 2404 44-45 0.0548311268478372 2404 46-47 0.14267213823167424 2404 48-49 0.013976780439350023 2404 50-51 -0.04252040345831176 2404 52-53 0.0909985710776624 2404 54-55 0.20417724532373427 2404 56-57 0.02827349051067074 2404 58-59 0.0674698846076538 2404 60-61 0.2719013486972841 2404 62-63 0.2339757360874657 2404 64-65 0.06478487051197845 2404 66-67 0.30608480224753265 2404 68-69 0.16387394537011346 2404 70-71 0.027439704495577644 2404 72-73 0.06747682909634278 2404 74-75 0.39212382620708297 2404 76-77 0.1622984862551533 2404 78-79 0.30475262474465836 2404 80-81 0.07527303714130085 2404 82-83 0.08428499336634587 2404 84-85 0.35436075693415603 2404 86-87 0.6771094292386977 2404 88-89 0.19403554776599918 2404 90-91 -1.8908882783882817 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 18.0 31 378.0 32 2389.0 33 4528.0 34 5933.0 35 7450.0 36 10602.0 37 15802.0 38 41659.0 39 239627.0 40 249083.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.2767230795073 11.70902680490208 27.682871288328897 31.331378827261723 2 30.326930504103732 16.153418508107883 25.263205294067724 28.256445693720657 3 28.558416122770225 18.552337874414036 20.282474037567386 32.60677196524835 4 27.535157731410692 19.793270288102043 19.641400663931744 33.030171316555524 5 28.225064895258445 19.741146277982022 19.524857611404247 32.50893121535528 6 28.518413975468814 22.163094469140336 22.441377805561856 26.877113749828997 7 26.943610825862514 21.57847434234565 22.23928903542874 29.238625796363095 8 26.38410027204924 22.328990820286457 22.311327534465054 28.975581373199255 9 26.289203403126404 22.44830458431535 23.011798035911884 28.250693976646364 10-11 26.306260595806876 21.373874614914392 22.582857261601923 29.737007527676813 12-13 26.705935729883336 21.360540565813924 22.285785037811554 29.647738666491186 14-15 27.11825223518492 21.06675856193146 22.104476603938913 29.710512598944703 16-17 27.267610902057083 20.7961812668732 21.755020615825266 30.181187215244453 18-19 27.461387537686004 21.100440023620315 21.700731987344778 29.737440451348903 20-21 27.487016619073923 21.021821084768185 21.56574638638611 29.92541590977178 22-23 27.412986671145983 20.62500324692754 21.6418543679401 30.32015571398638 24-25 27.351078586036653 21.185812571757097 21.695017394873144 29.768091447333106 26-27 27.51680176771394 21.174556556282674 21.69146742076198 29.61717425524141 28-29 27.483206890759504 20.886662314340683 21.802728804489938 29.827401990409875 30-31 27.522698266740786 21.18198962018609 21.962207364230338 29.333104748842793 32-33 27.643864615471607 21.126266535197928 21.883183663948937 29.346685185381528 34-35 27.637359424283737 20.804383659774786 22.11255559982018 29.445701316121298 36-37 27.59252560636637 21.207497239462363 22.176350759623805 29.023626394547463 38-39 27.82779569489237 21.147828695717394 22.07595189879747 28.948423710592763 40-41 27.5201197710036 20.93377336895123 22.21946935262036 29.326637507424806 42-43 27.51463122965685 21.11331976463321 22.447185588366064 28.924863417343875 44-45 27.703272085141773 21.095808887105687 22.427764979627973 28.773154048124567 46-47 27.69393467128908 20.745735398577228 22.450383257529978 29.109946672603716 48-49 27.694261967843794 21.08271331615432 22.554204623815917 28.66882009218597 50-51 27.83216828074159 21.047601178362896 22.43604238129373 28.68418815960178 52-53 27.721748263740032 20.853875503729743 22.605140186915886 28.81923604561434 54-55 27.76328757902834 20.979670985680745 22.701066532048046 28.555974903242863 56-57 27.821951468073784 21.009817354183415 22.73051532616942 28.43771585157338 58-59 27.77189065731129 20.632156709431168 22.714225454994395 28.88172717826315 60-61 27.948701321669006 20.854309867125696 22.748387705294927 28.44860110591037 62-63 28.053775292212652 20.721584955981005 22.67998802574465 28.544651726061694 64-65 27.8349820054678 20.677245150205735 22.703663583590256 28.78410926073621 66-67 28.10833737275812 20.815466646780266 22.754856631492597 28.321339348969016 68-69 28.18928336729566 20.730272858302545 22.53552238476279 28.544921389639004 70-71 28.27415446212201 20.346249078073964 22.863107154146036 28.516489305657995 72-73 28.287953500559805 20.768320668270643 22.647037804616595 28.296688026552957 74-75 28.31842143444407 20.5639115925908 22.63287710983218 28.48478986313295 76-77 27.916512700702086 20.25198765093631 23.0138390568939 28.817660591467703 78-79 28.27130673346454 20.699886682173315 22.530864197530864 28.497942386831276 80-81 28.59664104753772 20.058829110921337 22.453743239396527 28.890786602144413 82-83 28.59876251770494 20.137663693064635 21.683274109783067 29.580299679447357 84-85 29.515597949692797 20.08214807019926 21.26684544621338 29.135408533894562 86-87 29.85796014993095 19.076740974551193 21.45393568751233 29.611363188005523 88-89 28.958350380492593 19.212830373946485 21.307585303984943 30.521233941575975 90-91 26.93386773547094 19.939879759519037 22.74549098196393 30.38076152304609 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 1.5 10 2.0 11 1.0 12 3.0 13 6.5 14 12.0 15 14.0 16 13.5 17 24.5 18 40.5 19 55.0 20 90.5 21 143.0 22 190.5 23 259.5 24 368.5 25 497.0 26 652.5 27 868.0 28 1163.0 29 1495.5 30 1856.5 31 2258.0 32 2742.0 33 3237.5 34 3840.0 35 4640.0 36 5566.0 37 6568.5 38 7715.0 39 8997.5 40 10323.0 41 11870.0 42 13510.0 43 15027.5 44 16723.5 45 18787.5 46 20891.5 47 23029.0 48 25142.5 49 27482.5 50 29872.0 51 32014.0 52 34351.5 53 36599.0 54 38669.0 55 40686.5 56 42564.0 57 44181.5 58 45552.0 59 46629.5 60 47294.5 61 47597.5 62 47209.0 63 46139.0 64 44337.5 65 41824.0 66 38627.5 67 35473.0 68 32040.5 69 28064.0 70 24291.0 71 20554.0 72 17035.5 73 13858.0 74 10953.0 75 8629.5 76 6811.0 77 5141.5 78 3893.0 79 2849.0 80 1966.0 81 1383.5 82 971.5 83 667.0 84 470.5 85 350.5 86 240.5 87 161.0 88 121.5 89 81.5 90 50.5 91 33.0 92 20.5 93 14.0 94 10.5 95 10.0 96 9.0 97 6.5 98 4.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0117755238809356 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.05856714383231008 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.401765516302961E-4 64-65 7.935783638794873E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 7.94041512490273E-4 74-75 9.672489505348886E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 7396.0 32-33 17760.0 34-35 21209.0 36-37 24162.0 38-39 27459.0 40-41 29771.0 42-43 31507.0 44-45 32653.0 46-47 32910.0 48-49 32850.0 50-51 32004.0 52-53 30865.0 54-55 29311.0 56-57 26825.0 58-59 25275.0 60-61 23014.0 62-63 21566.0 64-65 19249.0 66-67 17167.0 68-69 15194.0 70-71 13338.0 72-73 11649.0 74-75 10120.0 76-77 8731.0 78-79 7499.0 80-81 6379.0 82-83 5626.0 84-85 4737.0 86-87 4202.0 88-89 3703.0 90-91 3338.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.9229143514976 #Duplication Level Percentage of deduplicated Percentage of total 1 99.92691585857443 99.8498865474564 2 0.0701125233866628 0.14011695338665756 3 0.0023915980660286044 0.0071692634614495074 4 3.253515337151918E-4 0.0013004029375020597 5 0.0 0.0 6 2.5466843914735796E-4 0.0015268327579770614 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0012121862818610177 0.0 0.0 0.0 0.0 2 0.0012121862818610177 0.0 0.0 0.0 0.0 3 0.0012121862818610177 0.0 0.0 0.0 0.0 4 0.0012121862818610177 0.0 0.0 0.0 0.0 5 0.0012121862818610177 0.0 0.0 0.0 0.0 6 0.0012121862818610177 0.0 0.0 0.0 0.0 7 0.0019048641572101705 0.0 0.0 0.0 1.7316946883728823E-4 8 0.006234100878142377 0.0 0.0 0.0 1.7316946883728823E-4 9 0.006753609284654241 0.0 0.0 0.0 1.7316946883728823E-4 10-11 0.006753609284654241 0.0 0.0 0.0 3.4633893767457646E-4 12-13 0.006753609284654241 0.0 8.658473441864411E-5 0.0 3.4633893767457646E-4 14-15 0.006753609284654241 1.7316946883728823E-4 1.7316946883728823E-4 0.0 3.4633893767457646E-4 16-17 0.006753609284654241 1.7316946883728823E-4 1.7316946883728823E-4 0.0 3.4633893767457646E-4 18-19 0.006753609284654241 1.7316946883728823E-4 1.7316946883728823E-4 0.0 3.4633893767457646E-4 20-21 0.006926778753491529 1.7316946883728823E-4 1.7316946883728823E-4 0.0 3.4633893767457646E-4 22-23 0.006926778753491529 1.7316946883728823E-4 1.7316946883728823E-4 0.0 3.4633893767457646E-4 24-25 0.006926778753491529 1.7316946883728823E-4 1.7316946883728823E-4 0.0 3.4633893767457646E-4 26-27 0.006926778753491529 1.7316946883728823E-4 1.7316946883728823E-4 0.0 5.195084065118647E-4 28-29 0.007099948222328818 1.7316946883728823E-4 1.7316946883728823E-4 0.0 5.195084065118647E-4 30-31 0.007273117691166106 1.7316946883728823E-4 1.7316946883728823E-4 0.0 6.926778753491529E-4 32-33 0.007619456628840682 1.7316946883728823E-4 1.7316946883728823E-4 0.0 6.926778753491529E-4 34-35 0.00779262609767797 1.7316946883728823E-4 1.7316946883728823E-4 0.0 6.926778753491529E-4 36-37 0.00796579556651526 3.4633893767457646E-4 1.7316946883728823E-4 0.0 6.926778753491529E-4 38-39 0.008312134504189835 3.4633893767457646E-4 1.7316946883728823E-4 0.0 8.658473441864412E-4 40-41 0.008485303973027124 3.4633893767457646E-4 2.5975420325593233E-4 0.0 8.658473441864412E-4 42-43 0.008571888707445768 3.4633893767457646E-4 3.4633893767457646E-4 0.0 8.658473441864412E-4 44-45 0.008658473441864411 3.4633893767457646E-4 3.4633893767457646E-4 0.0 8.658473441864412E-4 46-47 0.009004812379538988 3.4633893767457646E-4 3.4633893767457646E-4 0.0 8.658473441864412E-4 48-49 0.009004812379538988 3.4633893767457646E-4 3.4633893767457646E-4 0.0 8.658473441864412E-4 50-51 0.009004812379538988 3.4633893767457646E-4 3.4633893767457646E-4 0.0 8.658473441864412E-4 52-53 0.009004812379538988 3.4633893767457646E-4 3.4633893767457646E-4 0.0 8.658473441864412E-4 54-55 0.009004812379538988 3.4633893767457646E-4 3.4633893767457646E-4 0.0 8.658473441864412E-4 56-57 0.009004812379538988 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