##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-33B_S39_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1289998
Sequences flagged as poor quality	0
Sequence length	61
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.3107648228912	40.0	40.0	40.0	40.0	40.0
2	39.22719260029861	40.0	40.0	40.0	40.0	40.0
3	39.1792297352399	40.0	40.0	40.0	40.0	40.0
4	39.06600165271574	40.0	40.0	40.0	40.0	40.0
5	39.0802652407213	40.0	40.0	40.0	40.0	40.0
6	39.20041426420816	40.0	40.0	40.0	40.0	40.0
7	39.20659721953057	40.0	40.0	40.0	40.0	40.0
8	39.22279569425689	40.0	40.0	40.0	40.0	40.0
9	39.25396473482905	40.0	40.0	40.0	40.0	40.0
10	39.26562366763359	40.0	40.0	40.0	40.0	40.0
11	39.24840813706688	40.0	40.0	40.0	40.0	40.0
12	39.254504270549255	40.0	40.0	40.0	40.0	40.0
13	39.15812582655167	40.0	40.0	40.0	40.0	40.0
14	39.29576324924535	40.0	40.0	40.0	40.0	40.0
15	39.24730115860645	40.0	40.0	40.0	40.0	40.0
16	39.25874613759091	40.0	40.0	40.0	40.0	40.0
17	39.299260929086714	40.0	40.0	40.0	40.0	40.0
18	39.24551821010575	40.0	40.0	40.0	40.0	40.0
19	39.303967913128545	40.0	40.0	40.0	40.0	40.0
20	38.898885114550566	40.0	40.0	40.0	40.0	40.0
21	39.14631495552706	40.0	40.0	40.0	40.0	40.0
22	39.130166093280764	40.0	40.0	40.0	40.0	40.0
23	39.265629869193596	40.0	40.0	40.0	40.0	40.0
24	39.2730810435365	40.0	40.0	40.0	40.0	40.0
25	39.00932869663364	40.0	40.0	40.0	40.0	40.0
26	39.227037561298545	40.0	40.0	40.0	40.0	40.0
27	39.23971354994349	40.0	40.0	40.0	40.0	40.0
28	38.938289826805935	40.0	40.0	40.0	40.0	40.0
29	39.192519678325084	40.0	40.0	40.0	40.0	40.0
30	38.79157021948871	40.0	40.0	40.0	40.0	40.0
31	39.008913192113475	40.0	40.0	40.0	40.0	40.0
32	39.03316439250293	40.0	40.0	40.0	40.0	40.0
33	39.07550244263945	40.0	40.0	40.0	40.0	40.0
34	38.83029896170382	40.0	40.0	40.0	40.0	40.0
35	38.8289718278633	40.0	40.0	40.0	40.0	40.0
36	39.038454323185	40.0	40.0	40.0	40.0	40.0
37	38.82976562754361	40.0	40.0	40.0	40.0	40.0
38	38.85273310501257	40.0	40.0	40.0	40.0	40.0
39	38.82420282822144	40.0	40.0	40.0	40.0	40.0
40	38.697700306512104	40.0	40.0	40.0	40.0	40.0
41	38.67234832922222	40.0	40.0	40.0	40.0	40.0
42	38.437842539290756	40.0	40.0	40.0	40.0	40.0
43	38.79681363847076	40.0	40.0	40.0	40.0	40.0
44	38.29129967643361	40.0	40.0	40.0	40.0	40.0
45	38.6789653937448	40.0	40.0	40.0	40.0	40.0
46	38.24712596453638	40.0	40.0	40.0	40.0	40.0
47	37.68868788943859	40.0	40.0	40.0	24.0	40.0
48	38.13088702463104	40.0	40.0	40.0	40.0	40.0
49	37.71762746918987	40.0	40.0	40.0	24.0	40.0
50	38.03903261865522	40.0	40.0	40.0	40.0	40.0
51	37.60374822286546	40.0	40.0	40.0	24.0	40.0
52	37.62835911373506	40.0	40.0	40.0	24.0	40.0
53	36.817446228598804	40.0	40.0	40.0	24.0	40.0
54	36.096105575357484	40.0	40.0	40.0	24.0	40.0
55	36.05317837702074	40.0	40.0	40.0	24.0	40.0
56	35.79942294484177	40.0	40.0	40.0	24.0	40.0
57	35.86925871202901	40.0	40.0	40.0	24.0	40.0
58	35.79159657611872	40.0	40.0	40.0	24.0	40.0
59	35.29912139398666	40.0	40.0	40.0	12.0	40.0
60	34.584621061428	40.0	40.0	40.0	12.0	40.0
61	34.399216122815695	40.0	24.0	40.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.09854839918596525
1101	2	-0.2183949392886504
1101	3	-0.08415605536509219
1101	4	-0.025484692546832832
1101	5	-0.09348069257098501
1101	6	0.027939326121696695
1101	7	-0.006224067488027174
1101	8	-0.05467247373481143
1101	9	-0.05056874416339241
1101	10	0.0017333465624886912
1101	11	-0.017517954492703325
1101	12	-0.01639371959333147
1101	13	0.0019239094308147742
1101	14	-0.04205171417441278
1101	15	-0.05898643569543083
1101	16	-0.061723103983474914
1101	17	-0.012260566090382952
1101	18	-0.05320637050953536
1101	19	6.252423371009286E-4
1101	20	0.04282645431763399
1101	21	0.0949034721703299
1101	22	0.005525605379887111
1101	23	-0.03691978486809688
1101	24	-0.016632872632385443
1101	25	0.09001363767317372
1101	26	-0.06378913526098984
1101	27	-0.008939177809821786
1101	28	0.0793462934320388
1101	29	-0.060623607856406636
1101	30	0.0537085527444674
1101	31	0.08242369631938118
1101	32	0.07721163848478341
1101	33	0.00841757395177467
1101	34	0.1651637149913725
1101	35	0.09424796668747604
1101	36	0.03475980307465676
1101	37	0.0632220653813036
1101	38	0.03869253383386706
1101	39	0.013084900496835417
1101	40	0.04956289217975751
1101	41	0.0442634772677053
1101	42	0.1615100885917684
1101	43	0.003672755335315969
1101	44	-0.08592748954424678
1101	45	0.03838280370267455
1101	46	-0.1788269074386335
1101	47	-0.059630929942940725
1101	48	0.13672280342315446
1101	49	0.06355036897192434
1101	50	0.061018804534526794
1101	51	0.2313344867229361
1101	52	0.15036882473946633
1101	53	-0.07292037927532391
1101	54	0.027356807821789175
1101	55	-0.11553017367604923
1101	56	0.1445619920774277
1101	57	0.01747265858421798
1101	58	0.3447512485457054
1101	59	0.3885656187395554
1101	60	0.19055475510725728
1101	61	0.18122211535865773
1102	1	-0.021800682398470883
1102	2	-0.026942863212951806
1102	3	0.15193400206829466
1102	4	0.046374124890320445
1102	5	0.0350559329821607
1102	6	-0.0863083906657991
1102	7	0.0446409640532579
1102	8	-0.00928597528834274
1102	9	0.05215816479056201
1102	10	0.010732028419184303
1102	11	-0.06790633600103746
1102	12	0.09649583882866608
1102	13	0.10095413257364783
1102	14	-0.03337079900634876
1102	15	0.025603056819399228
1102	16	0.05668853333880719
1102	17	-0.033263767295544255
1102	18	0.12595048854328184
1102	19	-0.024779615962494006
1102	20	-0.038264073881215666
1102	21	-0.002433172213443413
1102	22	0.10783706381129576
1102	23	-0.055153708227479115
1102	24	0.12044579227675456
1102	25	-0.0323159452286248
1102	26	0.058794660991715375
1102	27	-0.011188259817160429
1102	28	0.046828168961653205
1102	29	0.023224429051602158
1102	30	0.08055748410686192
1102	31	0.04783302689088487
1102	32	0.024245816057515412
1102	33	0.08563134854927057
1102	34	0.018176613965103172
1102	35	-0.021414400321276617
1102	36	0.03795159292495498
1102	37	0.10172479582099925
1102	38	0.01634412031347665
1102	39	0.0034565698311723736
1102	40	0.10162249108758203
1102	41	-0.010779597772305749
1102	42	-0.04044829574115738
1102	43	-0.0551894045366339
1102	44	0.07458705710860869
1102	45	0.07449286866830818
1102	46	0.15466805537089812
1102	47	-0.17371976338611717
1102	48	0.18565416581841276
1102	49	0.18080863090582966
1102	50	0.08171953892865247
1102	51	0.14044850611847437
1102	52	0.17202756685414755
1102	53	0.27128685168221267
1102	54	0.009344144230794882
1102	55	0.2315616726661034
1102	56	0.05997132264894134
1102	57	0.30580355962931804
1102	58	-0.03781159864382744
1102	59	0.029718758745204354
1102	60	0.1993004005267096
1102	61	-0.2202631496823031
1103	1	-0.06621545435302068
1103	2	-0.03202964469165437
1103	3	-0.11445837499667988
1103	4	-0.12079923572722606
1103	5	0.010756093677997569
1103	6	-0.001391031867200354
1103	7	0.0023762414171599744
1103	8	-0.05080357840250116
1103	9	-0.0400453583351279
1103	10	0.008042687627927592
1103	11	-0.0731687400215364
1103	12	-0.1413619762689038
1103	13	-0.017159987701859336
1103	14	0.003245536000889615
1103	15	-0.07773536968826988
1103	16	-0.008800823012414583
1103	17	-0.009414563854136304
1103	18	-0.05345131526667757
1103	19	-0.08060876755544655
1103	20	-0.00416290456197288
1103	21	-0.027687114749525676
1103	22	-0.06960563194647307
1103	23	-0.06026557864071691
1103	24	-0.059859936896032195
1103	25	-0.01917368584341972
1103	26	-0.011847817770892277
1103	27	-0.059447307891119294
1103	28	-0.01577234132074068
1103	29	-0.0409588552648259
1103	30	-0.001538334514613382
1103	31	-0.07459228905026549
1103	32	-0.036132876079243204
1103	33	-0.028625227420292276
1103	34	-0.020828477098383757
1103	35	0.0783867276555057
1103	36	0.016144001532026664
1103	37	-0.06769474815745724
1103	38	-0.09832847361397512
1103	39	0.013674520903528276
1103	40	-0.1387560382123425
1103	41	0.1106773817913691
1103	42	0.02122923123774001
1103	43	-0.015174725097530484
1103	44	0.16547727100685705
1103	45	0.025093721511154854
1103	46	-0.011340599708425714
1103	47	-0.034939598239411396
1103	48	0.22494271359428808
1103	49	0.1940751287649789
1103	50	0.15829337955300815
1103	51	0.09635808101482013
1103	52	0.09971619146488564
1103	53	0.09777105666001518
1103	54	0.11522161916221307
1103	55	0.24311609198240802
1103	56	0.22549870441658015
1103	57	0.34718606848056055
1103	58	0.07385466545400021
1103	59	0.07940956476642924
1103	60	0.1773374507314145
1103	61	0.32339572765422275
1104	1	-0.04989648577274863
1104	2	0.009393039064654829
1104	3	-0.006310094793001042
1104	4	-0.008674680950392144
1104	5	0.0030452061534091968
1104	6	-0.0979110797538354
1104	7	-0.03349020587225482
1104	8	-0.08538372228525759
1104	9	0.09594749085786702
1104	10	0.10711602920647323
1104	11	0.022982625329483142
1104	12	-0.05991903849553637
1104	13	0.09631896161481279
1104	14	0.026367303993346525
1104	15	-0.03031422850563814
1104	16	0.040615512174234425
1104	17	-0.043696592375653154
1104	18	0.00298806319745637
1104	19	0.03975357631468057
1104	20	-0.11250307795913272
1104	21	-0.04235493525139589
1104	22	0.023983278360176996
1104	23	-0.052086966932265
1104	24	-0.07152753826825631
1104	25	0.05118971034421804
1104	26	-0.09790683916512677
1104	27	0.004817103597218875
1104	28	0.03341471245020955
1104	29	0.008433214718003512
1104	30	-0.018445486683440038
1104	31	-0.1452966361120076
1104	32	-0.0554710055043941
1104	33	0.0328909021311361
1104	34	0.014545237990979842
1104	35	-0.18744897958233508
1104	36	0.09046138792196956
1104	37	-0.02585311822905112
1104	38	-0.07686119635554434
1104	39	0.045849685962629394
1104	40	-0.003906377244291548
1104	41	-0.03222956486603579
1104	42	0.026836721757597104
1104	43	0.17003340473616646
1104	44	0.06806361984887133
1104	45	0.05959463207531002
1104	46	-0.021443838246518965
1104	47	-0.20619548347985983
1104	48	-0.3017387350383842
1104	49	0.12284685138398999
1104	50	0.05693588996166454
1104	51	0.0896645988218907
1104	52	-0.04009793212176049
1104	53	0.196880548440177
1104	54	0.12871455811170307
1104	55	0.14308501766527115
1104	56	0.2507612576359932
1104	57	0.19023551130938188
1104	58	0.101071310393408
1104	59	0.028230288240905566
1104	60	-0.011218534417288595
1104	61	-0.2803260098829341
1201	1	-9.112495139333987E-4
1201	2	-0.042895535591576106
1201	3	-0.08145033085704512
1201	4	-0.034705026476466116
1201	5	0.03830970695197067
1201	6	-4.1487781390259215E-4
1201	7	0.012745819214416088
1201	8	0.060950662818555656
1201	9	0.07497538225282341
1201	10	-0.04951888957346995
1201	11	0.034643643599125085
1201	12	-0.01860004040430141
1201	13	0.07385294938310949
1201	14	0.05772467222988098
1201	15	0.014015949516256399
1201	16	0.046386615754151705
1201	17	-0.10696837765866007
1201	18	0.01518957463059678
1201	19	-0.09484285545657656
1201	20	0.017192882463128
1201	21	0.02983936960622202
1201	22	0.07524832452121188
1201	23	0.025833643873703238
1201	24	-0.007889581040259941
1201	25	0.03553391197251443
1201	26	0.06823978543071973
1201	27	0.011312760174675418
1201	28	-0.02798823250714122
1201	29	0.03241455553059325
1201	30	-0.010446187863763612
1201	31	-0.13888667935146515
1201	32	-0.03741269067442943
1201	33	-0.044213612691038406
1201	34	0.1010611508888033
1201	35	0.05201481224501947
1201	36	-0.001644490306375701
1201	37	0.01494389006824548
1201	38	0.058184185592956794
1201	39	0.072856814629219
1201	40	0.07071673833360137
1201	41	-0.06458416733575234
1201	42	-0.0247302214857541
1201	43	0.032876690934600106
1201	44	0.05211663687197188
1201	45	0.07007630400082832
1201	46	0.09487613370627201
1201	47	0.6952214850838914
1201	48	0.381788396744561
1201	49	0.13467439997968
1201	50	0.06944903709266725
1201	51	-0.03650987821445284
1201	52	0.0855730584902119
1201	53	-0.025663372715989397
1201	54	0.19177988474486796
1201	55	0.034999045167118936
1201	56	0.3135616939259691
1201	57	0.3071193491030044
1201	58	0.10151928670909172
1201	59	0.16495947681946177
1201	60	0.3869992989355211
1201	61	0.452584667535163
1202	1	-0.2870261379981969
1202	2	-0.2437198992642564
1202	3	-0.15147994802347853
1202	4	-0.15048183366141643
1202	5	-0.14102300407513724
1202	6	-0.1685556803584447
1202	7	-0.09233846102313947
1202	8	-0.012921311121409929
1202	9	-0.0495271569102087
1202	10	-0.07441401945108339
1202	11	4.148807592585513E-4
1202	12	0.034822259670221456
1202	13	-0.1866100541279252
1202	14	-0.09098058510526386
1202	15	-0.09844477220530479
1202	16	-0.06659845657934227
1202	17	-0.06403116912461115
1202	18	-0.13334474056366474
1202	19	-0.10185436428026406
1202	20	-0.06099778140699641
1202	21	-0.14178269844795466
1202	22	-0.15830377633849224
1202	23	-0.0826819637199705
1202	24	-0.036613622803919554
1202	25	-0.17062999273012025
1202	26	-0.12834943868402604
1202	27	0.005295212675811456
1202	28	-0.05950977121688794
1202	29	-0.0309560009340899
1202	30	5.075540404959611E-4
1202	31	0.04511428868043765
1202	32	0.08191164610848034
1202	33	-0.05634666329759597
1202	34	-0.06932206681849351
1202	35	-0.08738274155373205
1202	36	0.006681736443567843
1202	37	-0.02418073251455155
1202	38	-0.12228653912489307
1202	39	-0.06629777084569355
1202	40	-0.02120595740475295
1202	41	-0.08460840654718993
1202	42	0.006505133190380263
1202	43	-0.03915525411806442
1202	44	-0.07932346423358183
1202	45	0.04741878754044393
1202	46	0.013233014799681087
1202	47	-0.07905133857295965
1202	48	0.20747831154238838
1202	49	0.15354045816915374
1202	50	0.011179855079625156
1202	51	-0.04763936400512847
1202	52	0.05498401876249659
1202	53	-0.012111886479715395
1202	54	-0.044344991370095954
1202	55	-0.041728046665305385
1202	56	0.33257468552867664
1202	57	0.15800498304865584
1202	58	0.30096074766898084
1202	59	0.4057103545785452
1202	60	0.08174081137012479
1202	61	-0.18381371600522556
1203	1	-0.037773939506671184
1203	2	-0.016302406946877568
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2403	10	-0.005615571498957195
2403	11	0.14706636832204367
2403	12	0.0490317415841659
2403	13	0.036025396977599655
2403	14	0.04298732680436501
2403	15	0.13255991003095602
2403	16	-0.0679535122514281
2403	17	0.030312623871047606
2403	18	0.11152233743354145
2403	19	0.007384271157135913
2403	20	-0.022695184453574768
2403	21	0.012592312719753807
2403	22	0.09795203545758824
2403	23	0.03555276295947607
2403	24	0.054760111390258714
2403	25	0.09588664525339397
2403	26	0.003979972020957234
2403	27	0.11043906373129886
2403	28	0.07050507907711534
2403	29	0.07943281633190225
2403	30	-0.04897278357789503
2403	31	0.12723271849009166
2403	32	0.038391986908898446
2403	33	0.0959698185705804
2403	34	0.10560959906531053
2403	35	0.10138268181182042
2403	36	0.13527275024109997
2403	37	0.03397529029913926
2403	38	0.0023195927467938304
2403	39	0.07698933084051163
2403	40	0.10955348814837151
2403	41	0.12642217742158834
2403	42	0.12510603820895483
2403	43	0.14374960242007262
2403	44	-0.004357016302989791
2403	45	0.16611662211938238
2403	46	0.024779871063181247
2403	47	0.0560721401408415
2403	48	-0.4460212739368714
2403	49	-0.0017003102081147858
2403	50	0.013528683516994988
2403	51	0.008652722944603397
2403	52	0.051594910952587725
2403	53	0.01699023493141283
2403	54	-0.0275952696142312
2403	55	-0.21557268179771683
2403	56	-0.29609453280050957
2403	57	-0.48315688907236876
2403	58	-0.5528378997720154
2403	59	-0.4230709201969347
2403	60	-0.6300146637425996
2403	61	-0.3260289627802919
2404	1	-0.041450869989944295
2404	2	-0.01976908271110034
2404	3	-0.08357092844683933
2404	4	-0.02658859178436046
2404	5	-0.05102943304004981
2404	6	-0.05862809015086157
2404	7	-0.0404787243744309
2404	8	-0.05920388962130829
2404	9	-0.060817513587196004
2404	10	-0.06072442778361875
2404	11	-0.06543562653208568
2404	12	-0.052857381402034775
2404	13	-0.05129489184319169
2404	14	-0.11028837086777799
2404	15	-0.014248958617933738
2404	16	-0.08899363771574542
2404	17	-0.07287006324316536
2404	18	-0.11150914051243177
2404	19	-0.04976805946774476
2404	20	-0.016628472812953987
2404	21	0.039082364169168216
2404	22	-0.05021708354975374
2404	23	-0.13339816533330406
2404	24	0.025840102949118204
2404	25	-0.042986314068933495
2404	26	-0.07371940260970433
2404	27	-0.03539777254005827
2404	28	-0.1449829218946661
2404	29	-0.05881393697521986
2404	30	-0.006873196121475189
2404	31	-0.06756544565533318
2404	32	-0.057172108257809384
2404	33	-0.004637391785124123
2404	34	-0.06529183949178474
2404	35	-0.10042918802732004
2404	36	-0.09813277486183125
2404	37	-0.07053070944737527
2404	38	0.06821550458958114
2404	39	-0.04909177163905554
2404	40	0.0449325476184228
2404	41	0.07889695859190482
2404	42	-0.10172705068050192
2404	43	-0.11369767222959126
2404	44	0.022973678296693834
2404	45	-0.003058659476550929
2404	46	-0.1185769315675742
2404	47	-0.35962007191765366
2404	48	-0.4977236785766479
2404	49	-0.1494985645286988
2404	50	-0.0560922368707395
2404	51	-0.23766464463504633
2404	52	-0.23580167038648625
2404	53	0.10027960142151926
2404	54	-0.27202187184233395
2404	55	-0.13517958010400122
2404	56	-0.46295538076302734
2404	57	-0.605796089606109
2404	58	-0.09350760677713765
2404	59	-0.060219488875539184
2404	60	0.14823620477427824
2404	61	0.06742277166596011
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	26.0
15	108.0
16	424.0
17	927.0
18	1293.0
19	1372.0
20	1469.0
21	1515.0
22	1633.0
23	2035.0
24	2514.0
25	2919.0
26	3507.0
27	4517.0
28	6910.0
29	8689.0
30	10238.0
31	10688.0
32	12135.0
33	14391.0
34	20173.0
35	32389.0
36	50203.0
37	83622.0
38	175259.0
39	473820.0
40	367217.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.947192734117124	28.30979539122494	20.171724391344323	21.57128748331362
2	28.958494509293814	19.57917764213588	20.283829897410694	31.178497951159613
3	31.35687032072918	18.757936058815595	17.318709021254296	32.566484599200926
4	30.928265005062023	18.96002939539441	19.380727722058484	30.73097787748508
5	28.42174949108448	20.117318011345752	20.212046840382698	31.248885657187063
6	27.94523712439864	20.27305468690649	21.834219898015345	29.94748829067952
7	28.836866413746375	22.373833137725796	22.127243608129625	26.6620568403982
8	26.84756100397055	24.077014072890034	23.371043986114707	25.704380937024705
9	27.132832764081805	23.707788694246037	22.993446501467442	26.165932040204716
10	29.083068345842396	22.797554724891047	22.13220485613156	25.987172073134996
11	27.60833737726725	23.696470847241624	22.30856171870034	26.386630056790782
12	28.76950196822011	22.031894623092438	22.07491794560922	27.12368546307824
13	28.739036804708224	22.647244414332427	21.397397515344984	27.21632126561436
14	29.281673304919853	22.517941888281996	21.619800960931723	26.58058384586643
15	28.414617697081702	21.994995341078045	22.47577128026555	27.1146156815747
16	28.92082003227912	22.55569388479672	21.016234133696333	27.50725194922783
17	29.639038200059225	22.762283352377292	21.272823678796403	26.325854768767083
18	28.511904669619643	21.96530537256647	22.176468490648823	27.346321467165062
19	30.229736790289596	22.386778894230844	20.970110031178343	26.413374284301216
20	28.602757523655075	22.20980187566182	22.41546110924203	26.771979491441073
21	28.75345543171385	22.898019996930227	21.283211291800452	27.06531327955547
22	30.162527383763386	22.37522848872634	20.962048003175198	26.50019612433508
23	29.773922130111828	22.33848424571201	21.427087483856564	26.460506140319595
24	29.817798167128938	21.858018384524627	21.05251325971048	27.271670188635948
25	29.07725438334013	23.551276823684997	20.92290065565993	26.448568137314943
26	28.542912469631737	23.552904733185635	21.389490526341902	26.51469227084073
27	28.874153293260918	23.257245360070325	21.17956772026003	26.689033626408726
28	29.15454132487027	23.980734853852486	20.81235784861682	26.052365972660425
29	28.687021220187937	23.979184463851883	21.213211183273152	26.120583132687024
30	28.759889550216357	23.67864136223467	20.984141060683815	26.57732802686516
31	29.43415416147932	23.32840826109808	21.641273862440098	25.596163714982506
32	29.919581270668637	23.53484268967859	20.974218564679944	25.571357474972828
33	29.208443476306016	23.491112334204143	21.54698873634679	25.753455453143047
34	29.65376690506497	24.288409749472482	20.159953736362382	25.897869609100173
35	29.16415374287402	25.419729332913693	20.50925660349861	24.906860320713676
36	29.519038014012427	24.177014227929035	20.253209694898754	26.05073806315979
37	30.05655822722206	25.58058229547643	19.712123584687728	24.65073589261379
38	29.94337975717792	25.989497658135903	19.944294487278274	24.122828097407904
39	30.289504324812906	24.922209181719662	19.37940989055797	25.408876602909462
40	30.669504913961106	25.31724855387373	19.218091810995055	24.79515472117011
41	30.605396287436104	26.02562174514999	19.37661918855688	23.99236277885702
42	31.57113421881274	25.597326507482958	19.294370999024803	23.537168274679495
43	31.51818840029209	25.854303650083178	19.717317391189752	22.910190558434973
44	31.50702559228774	25.918877393608362	19.959953426284383	22.614143587819516
45	32.22532127956788	25.627404073494688	19.346929220045304	22.800345426892136
46	32.70873288175641	25.683683230516635	18.12723740656962	23.480346481157337
47	32.9764077153608	25.79166789405875	18.13212113507153	23.099803255508924
48	33.1522994609294	26.837871066466768	18.000803102020313	22.00902637058352
49	33.97036274474844	25.983838734633697	17.665066147389375	22.380732373228483
50	33.68571114063742	26.531048885347108	18.10266372506004	21.680576248955425
51	33.95989761224436	26.56849080386171	17.82576407095205	21.64584751294188
52	34.14555681481677	26.9653906440165	17.811810560946604	21.077241980220123
53	34.8323020655846	25.676861514513977	17.1335924551821	22.357243964719327
54	34.60850326899732	25.48407051793879	16.984755015124055	22.92267119793984
55	35.65633435090597	25.76190040604714	17.837857112956765	20.743908130090123
56	34.394394409913815	26.72686314242348	19.27940973551897	19.59933271214374
57	35.478892215336764	27.878027717872435	17.821035381450205	18.822044685340597
58	34.312998934882074	28.74996705421249	18.5394861077304	18.39754790317504
59	33.81997491468979	29.60485210054589	17.564833433850286	19.010339550914033
60	33.80137023468253	27.286553932641755	20.30855861792034	18.60351721475537
61	33.95191310374124	29.586557498538756	17.551345040845025	18.91018435687497
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	31.0
2	34.5
3	39.0
4	36.0
5	33.0
6	31.0
7	27.0
8	25.0
9	25.5
10	26.0
11	21.5
12	17.5
13	18.0
14	23.0
15	28.0
16	34.5
17	51.0
18	61.0
19	68.5
20	107.0
21	138.0
22	182.5
23	227.0
24	341.0
25	643.5
26	832.0
27	1057.5
28	1283.0
29	1854.5
30	2934.5
31	3443.0
32	4274.0
33	5105.0
34	5736.0
35	7855.5
36	9344.0
37	10971.5
38	12599.0
39	15536.5
40	21137.0
41	23800.0
42	26988.5
43	34169.0
44	38161.0
45	41906.0
46	45651.0
47	50077.0
48	60015.5
49	65528.0
50	70910.0
51	76292.0
52	80127.0
53	88033.0
54	92104.0
55	94999.5
56	97895.0
57	99045.5
58	99353.0
59	98510.0
60	95806.5
61	88454.5
62	83806.0
63	77324.5
64	70843.0
65	63268.0
66	48247.0
67	40801.0
68	34810.0
69	28819.0
70	23814.0
71	15428.5
72	12048.0
73	9721.0
74	7394.0
75	5941.0
76	3480.0
77	2472.0
78	1941.5
79	1411.0
80	1078.0
81	591.0
82	437.0
83	337.0
84	174.0
85	111.0
86	104.0
87	97.0
88	76.0
89	52.0
90	49.0
91	51.5
92	54.0
93	54.5
94	51.5
95	48.0
96	53.5
97	59.0
98	73.5
99	218.5
100	349.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0018604680007255827
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	6.201560002418609E-4
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1289998.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.92483457123672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.63592810714852	67.58801459211017
2	10.774368684383047	17.007305320488907
3	2.0778326537675604	4.919777953959555
4	0.5803864973607963	1.8322763316632147
5	0.23151322356130022	0.9136071435314681
6	0.1306323050415854	0.6186079839039876
7	0.08450877369150553	0.46688886883941094
8	0.06305264056201092	0.398113538050909
9	0.048226586666258765	0.3425647837112895
>10	0.3678810500276021	5.404894822378878
>50	0.004204179478842967	0.21420165731655988
>100	0.0012559699808185269	0.18602875509303635
>500	2.0932833013642113E-4	0.10771824895262852
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.028141128900975	0.0	0.0	0.0	0.0
2	1.0298465579016403	0.0	0.0	0.0	0.0
3	1.030776791902003	0.0	0.0	0.0	0.0
4	1.0316295064023355	0.0	0.0	0.0	0.0
5	1.032482220902668	0.0	0.0	1.5503900006046521E-4	0.0
6	1.0362031569041192	7.751950003023261E-5	0.0	1.5503900006046521E-4	0.0
7	1.0393039369053285	7.751950003023261E-5	0.0	1.5503900006046521E-4	0.0
8	1.0533349664108007	7.751950003023261E-5	0.0	1.5503900006046521E-4	0.0
9	1.0573659804123727	7.751950003023261E-5	0.0	1.5503900006046521E-4	0.0
10	1.0593814874131589	7.751950003023261E-5	0.0	1.5503900006046521E-4	0.0
11	1.0668233594160612	7.751950003023261E-5	0.0	1.5503900006046521E-4	0.0
12	1.080389271921352	7.751950003023261E-5	0.0	1.5503900006046521E-4	0.0
13	1.1006218614292425	7.751950003023261E-5	0.0	1.5503900006046521E-4	0.0
14	1.1315521419413053	7.751950003023261E-5	0.0	1.5503900006046521E-4	0.0
15	1.1669010339550914	7.751950003023261E-5	7.751950003023261E-5	1.5503900006046521E-4	0.0
16	1.214032889973473	7.751950003023261E-5	7.751950003023261E-5	1.5503900006046521E-4	0.0
17	1.255118224989496	7.751950003023261E-5	7.751950003023261E-5	1.5503900006046521E-4	0.0
18	1.3033353540083008	7.751950003023261E-5	7.751950003023261E-5	2.325585000906978E-4	0.0
19	1.3568238090291613	7.751950003023261E-5	7.751950003023261E-5	2.325585000906978E-4	0.0
20	1.4265138395563404	7.751950003023261E-5	7.751950003023261E-5	2.325585000906978E-4	0.0
21	1.524343448594494	7.751950003023261E-5	7.751950003023261E-5	2.325585000906978E-4	0.0
22	1.6648863021493057	7.751950003023261E-5	7.751950003023261E-5	2.325585000906978E-4	0.0
23	1.852328453222408	7.751950003023261E-5	7.751950003023261E-5	2.325585000906978E-4	0.0
24	2.1086854398223873	7.751950003023261E-5	1.5503900006046521E-4	2.325585000906978E-4	0.0
25	2.4362828469501503	7.751950003023261E-5	1.5503900006046521E-4	2.325585000906978E-4	0.0
26	2.846748599610232	7.751950003023261E-5	1.5503900006046521E-4	4.651170001813956E-4	3.1007800012093043E-4
27	3.3674470813133044	7.751950003023261E-5	1.5503900006046521E-4	4.651170001813956E-4	3.1007800012093043E-4
28	3.9679131285474862	7.751950003023261E-5	1.5503900006046521E-4	4.651170001813956E-4	3.8759750015116305E-4
29	4.703650703334424	7.751950003023261E-5	1.5503900006046521E-4	4.651170001813956E-4	3.8759750015116305E-4
30	5.563031880669582	7.751950003023261E-5	1.5503900006046521E-4	4.651170001813956E-4	3.8759750015116305E-4
31	6.526986863545525	7.751950003023261E-5	3.1007800012093043E-4	4.651170001813956E-4	3.8759750015116305E-4
32	7.662492499988372	7.751950003023261E-5	3.1007800012093043E-4	4.651170001813956E-4	3.8759750015116305E-4
33	8.934044858984278	7.751950003023261E-5	3.1007800012093043E-4	4.651170001813956E-4	3.8759750015116305E-4
34	10.299318293516734	7.751950003023261E-5	3.1007800012093043E-4	4.651170001813956E-4	3.8759750015116305E-4
35	11.82451445661156	7.751950003023261E-5	3.1007800012093043E-4	4.651170001813956E-4	3.8759750015116305E-4
36	13.47056352025352	7.751950003023261E-5	3.1007800012093043E-4	4.651170001813956E-4	3.8759750015116305E-4
37	15.22374453293726	7.751950003023261E-5	3.1007800012093043E-4	4.651170001813956E-4	3.8759750015116305E-4
38	17.04638301764809	7.751950003023261E-5	3.1007800012093043E-4	4.651170001813956E-4	3.8759750015116305E-4
39	19.023982982919353	7.751950003023261E-5	3.8759750015116305E-4	4.651170001813956E-4	3.8759750015116305E-4
40	21.067784601216434	7.751950003023261E-5	4.651170001813956E-4	4.651170001813956E-4	3.8759750015116305E-4
41	23.229725937559593	7.751950003023261E-5	4.651170001813956E-4	4.651170001813956E-4	3.8759750015116305E-4
42	25.45376039342697	1.5503900006046521E-4	4.651170001813956E-4	4.651170001813956E-4	3.8759750015116305E-4
43	27.73058562881493	1.5503900006046521E-4	4.651170001813956E-4	4.651170001813956E-4	5.426365002116282E-4
44	30.07454275122907	1.5503900006046521E-4	4.651170001813956E-4	4.651170001813956E-4	5.426365002116282E-4
45	32.42353864114518	1.5503900006046521E-4	4.651170001813956E-4	4.651170001813956E-4	5.426365002116282E-4
46	34.79904620007163	1.5503900006046521E-4	4.651170001813956E-4	4.651170001813956E-4	5.426365002116282E-4
47	37.19649177750663	1.5503900006046521E-4	4.651170001813956E-4	4.651170001813956E-4	5.426365002116282E-4
48	39.553472175925855	1.5503900006046521E-4	4.651170001813956E-4	4.651170001813956E-4	5.426365002116282E-4
49	41.975646473870505	1.5503900006046521E-4	4.651170001813956E-4	4.651170001813956E-4	5.426365002116282E-4
50	44.33735556179157	1.5503900006046521E-4	4.651170001813956E-4	4.651170001813956E-4	6.201560002418609E-4
>>END_MODULE