##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-33B_S39_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1289998
Sequences flagged as poor quality	0
Sequence length	61
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.46841157893268	40.0	40.0	40.0	40.0	40.0
2	39.22065925683606	40.0	40.0	40.0	40.0	40.0
3	39.159390944792165	40.0	40.0	40.0	40.0	40.0
4	39.04116130412605	40.0	40.0	40.0	40.0	40.0
5	39.08710866218397	40.0	40.0	40.0	40.0	40.0
6	39.200783257028306	40.0	40.0	40.0	40.0	40.0
7	39.210253039152	40.0	40.0	40.0	40.0	40.0
8	39.22009491487584	40.0	40.0	40.0	40.0	40.0
9	39.22158018849642	40.0	40.0	40.0	40.0	40.0
10	39.22656314195836	40.0	40.0	40.0	40.0	40.0
11	39.22156158381641	40.0	40.0	40.0	40.0	40.0
12	39.222184840596654	40.0	40.0	40.0	40.0	40.0
13	39.04680162294825	40.0	40.0	40.0	40.0	40.0
14	39.21843909835519	40.0	40.0	40.0	40.0	40.0
15	39.126507172879336	40.0	40.0	40.0	40.0	40.0
16	39.31262529089192	40.0	40.0	40.0	40.0	40.0
17	39.25945931699119	40.0	40.0	40.0	40.0	40.0
18	39.352978841827664	40.0	40.0	40.0	40.0	40.0
19	39.301508994587586	40.0	40.0	40.0	40.0	40.0
20	39.30701908064974	40.0	40.0	40.0	40.0	40.0
21	39.28631207180166	40.0	40.0	40.0	40.0	40.0
22	39.34496022474453	40.0	40.0	40.0	40.0	40.0
23	39.33388423858022	40.0	40.0	40.0	40.0	40.0
24	39.33910285132225	40.0	40.0	40.0	40.0	40.0
25	39.29295704334425	40.0	40.0	40.0	40.0	40.0
26	39.261992654252175	40.0	40.0	40.0	40.0	40.0
27	39.2828174927403	40.0	40.0	40.0	40.0	40.0
28	39.276984925558025	40.0	40.0	40.0	40.0	40.0
29	39.296088831145475	40.0	40.0	40.0	40.0	40.0
30	39.28786246180227	40.0	40.0	40.0	40.0	40.0
31	39.235480985241836	40.0	40.0	40.0	40.0	40.0
32	39.2638469206929	40.0	40.0	40.0	40.0	40.0
33	39.25918024679108	40.0	40.0	40.0	40.0	40.0
34	39.23213524362053	40.0	40.0	40.0	40.0	40.0
35	39.25398954106905	40.0	40.0	40.0	40.0	40.0
36	39.23262826764073	40.0	40.0	40.0	40.0	40.0
37	39.24543448904572	40.0	40.0	40.0	40.0	40.0
38	39.228395702939075	40.0	40.0	40.0	40.0	40.0
39	37.97064491572855	40.0	40.0	40.0	40.0	40.0
40	39.230547644259914	40.0	40.0	40.0	40.0	40.0
41	39.23918021578328	40.0	40.0	40.0	40.0	40.0
42	39.209713503431786	40.0	40.0	40.0	40.0	40.0
43	39.216020489954246	40.0	40.0	40.0	40.0	40.0
44	39.18527935702226	40.0	40.0	40.0	40.0	40.0
45	39.081071443521616	40.0	40.0	40.0	40.0	40.0
46	39.06426831669506	40.0	40.0	40.0	40.0	40.0
47	39.14629325006705	40.0	40.0	40.0	40.0	40.0
48	39.23607323422207	40.0	40.0	40.0	40.0	40.0
49	39.205313496610074	40.0	40.0	40.0	40.0	40.0
50	39.19562045832629	40.0	40.0	40.0	40.0	40.0
51	39.15318628400974	40.0	40.0	40.0	40.0	40.0
52	39.162175445233245	40.0	40.0	40.0	40.0	40.0
53	39.09074587712539	40.0	40.0	40.0	40.0	40.0
54	38.98773486470522	40.0	40.0	40.0	40.0	40.0
55	39.03851943956502	40.0	40.0	40.0	40.0	40.0
56	39.05405124659108	40.0	40.0	40.0	40.0	40.0
57	38.97163871571894	40.0	40.0	40.0	40.0	40.0
58	38.95959218541424	40.0	40.0	40.0	40.0	40.0
59	38.921595227279425	40.0	40.0	40.0	40.0	40.0
60	38.87210057690012	40.0	40.0	40.0	40.0	40.0
61	38.72840423008408	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.051245695579900996
1101	2	-0.016581005390051473
1101	3	-0.015716492163051043
1101	4	-0.06276601932097492
1101	5	-0.04198793785969457
1101	6	0.10552559859962685
1101	7	-0.0328909384619962
1101	8	0.056416347919082455
1101	9	-0.014448310746011828
1101	10	-0.12312464835432735
1101	11	0.025350960547825707
1101	12	-0.1205024103296708
1101	13	5.484627912437645E-4
1101	14	0.0014656319388279826
1101	15	0.032985486626905924
1101	16	-0.05006586994555562
1101	17	0.09923754581505762
1101	18	0.010772976041764082
1101	19	-0.06990997856112813
1101	20	-0.08857271235627451
1101	21	0.04846563928096259
1101	22	-0.08308585638221899
1101	23	0.018458044372003712
1101	24	-0.059810791630958704
1101	25	0.06517166642487382
1101	26	0.007310903023402204
1101	27	0.08029787120914023
1101	28	0.004894443339559018
1101	29	-0.045345413749302566
1101	30	-0.026637061418874453
1101	31	-0.05094014167416816
1101	32	-0.13961453128806767
1101	33	-0.09486837315186136
1101	34	-0.027974415363004823
1101	35	-0.1060198974978519
1101	36	-0.10358232216176333
1101	37	-0.06157622965562126
1101	38	-0.03258255341792449
1101	39	1.1107070292533052
1101	40	-0.09979244183761438
1101	41	-0.1279841483074904
1101	42	-0.012729378632997168
1101	43	-0.13434010706853172
1101	44	0.031059022995378882
1101	45	0.01036797526578681
1101	46	-0.1494793381390167
1101	47	-0.09234306356329114
1101	48	-0.0128730038081315
1101	49	-0.043901872932650576
1101	50	-0.056235824315677974
1101	51	0.018537457652485045
1101	52	0.002534235150896791
1101	53	-0.08038318153688095
1101	54	-0.035706940272930865
1101	55	-0.10047282269771074
1101	56	0.003682122408619648
1101	57	-0.016237870751851347
1101	58	-0.05739859884879195
1101	59	0.012120434365485266
1101	60	-0.11041723933554692
1101	61	0.06104403301770844
1102	1	0.07309640968325226
1102	2	0.025149410766623248
1102	3	0.07920277155976407
1102	4	-0.11982632437699436
1102	5	-0.006553421335823373
1102	6	-0.028428360537930075
1102	7	-0.06749808468174479
1102	8	-0.07051614431328801
1102	9	-0.024151963886012595
1102	10	-0.09741967707102361
1102	11	-0.06465468863775214
1102	12	-0.027392062904759484
1102	13	-0.009950783984010059
1102	14	-0.10494854751696892
1102	15	-0.04819095626924508
1102	16	-0.033427841511318945
1102	17	0.04263152943241977
1102	18	-0.09391937872708667
1102	19	0.03053254097563496
1102	20	-0.05400087354637151
1102	21	0.05398065669928798
1102	22	0.032309351225350724
1102	23	-0.01583839664108666
1102	24	0.07664175521020411
1102	25	-0.02988765002367444
1102	26	-0.0021008755285194525
1102	27	-0.028028792946777514
1102	28	-0.12002670344511301
1102	29	0.06347790797181574
1102	30	-0.1508967827257237
1102	31	-0.03552021887970369
1102	32	-0.13221409818383734
1102	33	0.06600983982243491
1102	34	0.06415791680910843
1102	35	-0.06088055092031652
1102	36	-0.10679411954431117
1102	37	-0.1041581428464653
1102	38	-0.07367331699951052
1102	39	1.13845865339416
1102	40	-0.004176445227123793
1102	41	0.0379571472390694
1102	42	0.039061883959981
1102	43	0.004598081425960743
1102	44	0.022581713890772903
1102	45	0.016241951350899342
1102	46	0.0019835655423676712
1102	47	0.09600431427307399
1102	48	0.027573988000547445
1102	49	0.10609930398101142
1102	50	4.2433009539166733E-4
1102	51	-0.04946814445654013
1102	52	0.09980026471496473
1102	53	0.09257016560557219
1102	54	0.06136254470965241
1102	55	0.09517730440897054
1102	56	-0.050916079267302905
1102	57	0.02622635107282889
1102	58	-0.03333659903709929
1102	59	-0.012530022277012165
1102	60	-0.013340692870997373
1102	61	0.003963720429439377
1103	1	-0.03038772563656522
1103	2	-0.11812541287957856
1103	3	-0.033092645819692734
1103	4	-0.07651727484052628
1103	5	0.01025399365052948
1103	6	-0.043514047391241206
1103	7	-0.0010222989714065989
1103	8	0.0067934775645071
1103	9	-0.0894210683407195
1103	10	-0.017241473263837293
1103	11	-0.06998608722825139
1103	12	-0.00202353148493728
1103	13	-0.08505499201952915
1103	14	-0.0879780544560802
1103	15	0.015824666417309174
1103	16	-0.15833358461571123
1103	17	-0.017936777151945194
1103	18	-0.10555943815650437
1103	19	-0.01711182820200463
1103	20	-0.09131945748107029
1103	21	-0.11478175018594072
1103	22	-0.10482411135217973
1103	23	-0.13743650146157194
1103	24	-0.07792272712094217
1103	25	-0.07601269122289978
1103	26	-0.061011864994483744
1103	27	-0.09477959197808161
1103	28	-0.2013454888150079
1103	29	-0.1553926497111746
1103	30	-0.07563335645175329
1103	31	-0.10665820409954563
1103	32	-0.1476384856534878
1103	33	-0.08811486243493505
1103	34	-0.04283384455170136
1103	35	-0.15570104291070663
1103	36	-0.05521947227452273
1103	37	-0.06833210927734967
1103	38	-0.05244108242279566
1103	39	1.220651691636995
1103	40	-0.05546715330500973
1103	41	-0.059261509158019976
1103	42	0.014522976058380266
1103	43	-0.14226385676057163
1103	44	-0.04160784265928896
1103	45	-0.08117669280326822
1103	46	-0.03355153288194401
1103	47	-0.07930437460996842
1103	48	-0.12796269054415887
1103	49	-0.00969891464432493
1103	50	0.06327186988713152
1103	51	-0.12712105050601963
1103	52	-0.18677181787467134
1103	53	-0.02099923892122746
1103	54	-0.14583779857451873
1103	55	-0.09672047748194501
1103	56	-0.09083836388015953
1103	57	-0.02159846544171984
1103	58	-0.0835212228372697
1103	59	-0.07082416168886141
1103	60	-0.09838036022549801
1103	61	0.05181193720024169
1104	1	-0.018519564926634757
1104	2	-0.050469196344820944
1104	3	-0.12883496916343518
1104	4	-0.1142783888609813
1104	5	-0.08997788760843406
1104	6	-0.026857470592489108
1104	7	-0.024671958949042505
1104	8	-0.013645499780956527
1104	9	0.06014098187092287
1104	10	-0.05397602060017448
1104	11	-0.0846026536771447
1104	12	-0.13817732335634503
1104	13	-0.1125506868028765
1104	14	-0.034937537755801884
1104	15	-0.03351045461777602
1104	16	-0.06174445517941507
1104	17	-0.10211054077171866
1104	18	-0.022996834549651624
1104	19	-0.16098071686805326
1104	20	0.01367409862169211
1104	21	0.02667398872046789
1104	22	-0.00868017412477684
1104	23	-0.032658113339635975
1104	24	0.07209392425617267
1104	25	0.027995400479760235
1104	26	0.0297548535452421
1104	27	-0.07438419295785081
1104	28	0.03044044256646572
1104	29	0.05186660789738795
1104	30	-0.06720045531141494
1104	31	-0.08672483436841105
1104	32	-0.00138492170167126
1104	33	0.11449092333054978
1104	34	-0.15037004736918647
1104	35	0.0013535079911335401
1104	36	-0.09561459893022572
1104	37	-0.10797650023930316
1104	38	-0.03048552983849362
1104	39	1.2353494722374592
1104	40	0.052724952828022253
1104	41	-0.060323730835513345
1104	42	-0.08535210766276435
1104	43	0.022380998459105683
1104	44	-0.04449663993465691
1104	45	-0.07486105449842029
1104	46	-0.06180539148265751
1104	47	-0.06355972378748476
1104	48	-0.006103579764840106
1104	49	-0.0900798783443264
1104	50	0.021426525896920623
1104	51	0.03755079356280788
1104	52	0.002935278830832999
1104	53	0.008972249464278548
1104	54	-0.12383024912488594
1104	55	-0.015509544769606975
1104	56	0.041249768330537506
1104	57	0.020827262607248542
1104	58	8.686991798114718E-4
1104	59	-0.0707119970222223
1104	60	-0.027869887190206555
1104	61	-0.07453772191077945
1201	1	0.03431631992397399
1201	2	0.13230465352467746
1201	3	0.10458016854058627
1201	4	-0.07461008610690101
1201	5	0.06595779857441642
1201	6	0.028527983811315494
1201	7	0.03828526905142127
1201	8	0.07985105274913451
1201	9	0.08888353183002096
1201	10	-0.02539805324401101
1201	11	0.09517803270047409
1201	12	0.052738496050771744
1201	13	0.004849297615336923
1201	14	0.045785846607877545
1201	15	0.05063605311467967
1201	16	0.06623556118145757
1201	17	0.07206849393670467
1201	18	0.06398555803341566
1201	19	0.011336294545607473
1201	20	0.05651524232470706
1201	21	0.03753838823743649
1201	22	0.045752843677412613
1201	23	0.10457164007861763
1201	24	4.381648389326642E-4
1201	25	0.05630166046184115
1201	26	-0.0157585662669959
1201	27	0.08324211688594119
1201	28	-0.006532211401051313
1201	29	0.014814097282297212
1201	30	-0.06920563625489251
1201	31	0.10942062159357135
1201	32	-0.0756759504890212
1201	33	-0.04128310183403272
1201	34	-0.0370456735621687
1201	35	-0.041857703103097776
1201	36	0.048601637289635846
1201	37	0.00857135531754949
1201	38	0.01161840066674813
1201	39	1.2346336839939198
1201	40	0.061082632700248496
1201	41	0.021188481388399794
1201	42	0.032380937407552324
1201	43	-0.018351734975503575
1201	44	-0.027326486843620046
1201	45	-0.01797877488328936
1201	46	-9.03011365018358E-4
1201	47	0.04861549338363602
1201	48	0.07116843924494276
1201	49	0.0251425516350281
1201	50	0.0378682305441842
1201	51	0.08773095795064023
1201	52	-0.0068500820822592345
1201	53	0.016672572786319506
1201	54	0.03069139007888566
1201	55	-0.04002261399168816
1201	56	-0.028853655766688746
1201	57	0.09192402549143708
1201	58	0.056249284275835976
1201	59	-0.0010653139946796841
1201	60	0.11850345237584037
1201	61	0.1163362214494299
1202	1	0.051616048721385255
1202	2	0.06309565466941791
1202	3	-0.05690445434952096
1202	4	-0.09533730086400993
1202	5	-0.06721093092212982
1202	6	-0.022485760705095004
1202	7	-0.036227257742318386
1202	8	0.03309165477278242
1202	9	0.05229999298197896
1202	10	0.008994929292995835
1202	11	-0.01628584664894106
1202	12	-0.07784593400124606
1202	13	-0.014616018416347742
1202	14	-0.13358239733615562
1202	15	-0.09019626224859678
1202	16	-0.025448007629478298
1202	17	0.05610172969856819
1202	18	-0.04542413457804173
1202	19	-0.0519180596772415
1202	20	-0.038318271554260264
1202	21	-0.06595267283308459
1202	22	-0.08050809469890652
1202	23	-0.07755952824368961
1202	24	0.011978680047015189
1202	25	-0.06661342162880146
1202	26	0.0553735316732471
1202	27	-0.005158978680320558
1202	28	-0.024288106023860223
1202	29	0.031044957524613892
1202	30	0.12006092592579165
1202	31	0.026148750603027793
1202	32	0.013819943058202
1202	33	0.04986651287390487
1202	34	0.07247061780007158
1202	35	0.07505999881990277
1202	36	-0.1421515453072999
1202	37	-0.22173956730904365
1202	38	-0.25135399505854394
1202	39	1.1428752079527058
1202	40	0.0012624868808401857
1202	41	-0.1243505049439122
1202	42	-0.12668264538606877
1202	43	-0.04542781767308668
1202	44	-0.027646283095954516
1202	45	-0.21174169157876577
1202	46	-0.005369595057523213
1202	47	-0.0855856114019744
1202	48	-0.09740548074645261
1202	49	-0.04404195831802582
1202	50	-0.15601569013865912
1202	51	0.04376521552395474
1202	52	-0.05187893096940854
1202	53	-0.09958440895168508
1202	54	-0.132682239502941
1202	55	-0.010665702167095503
1202	56	-0.015505763540907935
1202	57	0.024931032198516334
1202	58	-0.1949329054737845
1202	59	-0.1775275415436397
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2403	6	0.04235473019907232
2403	7	0.1797966672146174
2403	8	0.13922976813680066
2403	9	0.10600949460003761
2403	10	0.04107567651220734
2403	11	0.1284884873825618
2403	12	0.03555749050054402
2403	13	0.13665210661593363
2403	14	0.16079414610187115
2403	15	0.09963489673766901
2403	16	-0.005346138577884574
2403	17	0.08394768317091206
2403	18	0.06842942492752968
2403	19	-0.009990150303444523
2403	20	0.016395038465390144
2403	21	0.10645099178965722
2403	22	0.06712810907934141
2403	23	0.05125887838609344
2403	24	-0.005672780346117179
2403	25	0.03170423856993665
2403	26	0.014082518047054293
2403	27	0.08601746959050871
2403	28	0.1885525640531256
2403	29	0.1260361714474172
2403	30	0.01725293041196352
2403	31	0.07395631899038335
2403	32	0.15247278371848694
2403	33	0.029369092889133697
2403	34	0.14681779353948343
2403	35	0.004097035458052289
2403	36	0.06525495009919524
2403	37	0.03235270816378488
2403	38	0.2004165726720828
2403	39	1.2552811881149424
2403	40	0.010653394741218847
2403	41	0.10469747298552079
2403	42	0.143002805951987
2403	43	0.00957505716955609
2403	44	0.026422788027581134
2403	45	0.1455502139952003
2403	46	0.02135652826674317
2403	47	0.0626360860842965
2403	48	0.027881360992296322
2403	49	0.09400800984942492
2403	50	0.14442507011219163
2403	51	0.043366237788134754
2403	52	0.10410276109272587
2403	53	0.1288638785159364
2403	54	0.05181656980553129
2403	55	0.08931762888578021
2403	56	0.0636648816844172
2403	57	0.11015634814359743
2403	58	0.16662561415726884
2403	59	0.10117162459289375
2403	60	0.19006816066825394
2403	61	0.1820872155699007
2404	1	-0.013808430837116248
2404	2	-0.1422789351265621
2404	3	-0.1386594995947661
2404	4	0.03563302517279965
2404	5	-0.03954126282832249
2404	6	-0.04390254540192018
2404	7	-0.06280238523353887
2404	8	-0.050788555082554865
2404	9	0.02290208979447783
2404	10	-0.047923171662894504
2404	11	-0.027322526567317595
2404	12	0.04365404175024423
2404	13	-0.012853959754842492
2404	14	-0.02387217329232527
2404	15	-0.020317765954892764
2404	16	-0.053766043673959985
2404	17	-0.004706330258677838
2404	18	0.05714036335959349
2404	19	-0.02840819301919595
2404	20	-0.02160644462149719
2404	21	-5.764415992572935E-4
2404	22	-0.027901752531235502
2404	23	-0.052868323739183154
2404	24	-0.06969280108950215
2404	25	0.0027524983908975287
2404	26	0.104916105223964
2404	27	0.022949400984259682
2404	28	-0.0660482745448121
2404	29	-0.033651320515062366
2404	30	-0.01502741949241937
2404	31	-0.028444726173731283
2404	32	-0.011499484477916155
2404	33	-0.10856034303893836
2404	34	-0.050849861748488934
2404	35	0.06451624766188502
2404	36	0.01587887849454006
2404	37	-0.05067115292557389
2404	38	-0.09071642080524356
2404	39	1.2102099729846074
2404	40	-0.07686149887844351
2404	41	0.033043986042478934
2404	42	0.07534850960172434
2404	43	0.10463417147601461
2404	44	-0.0855790910862737
2404	45	-0.17298908486354492
2404	46	0.057888435241927994
2404	47	0.03495039266680067
2404	48	0.008923878732161938
2404	49	-0.07189989435915578
2404	50	-0.04183205054867045
2404	51	-0.00259346600353183
2404	52	-0.06326901743090474
2404	53	-0.03407611658175824
2404	54	-0.041085282131824385
2404	55	-0.03132891766923507
2404	56	-0.11720751179657185
2404	57	-0.07627430545893077
2404	58	0.02123401383337864
2404	59	-0.09836721161462947
2404	60	-0.10172599331682619
2404	61	-0.161140119091165
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	7.0
17	46.0
18	106.0
19	143.0
20	239.0
21	354.0
22	461.0
23	662.0
24	1023.0
25	1291.0
26	1738.0
27	2269.0
28	3078.0
29	3702.0
30	4738.0
31	5898.0
32	7842.0
33	9408.0
34	12103.0
35	15661.0
36	22895.0
37	32554.0
38	84165.0
39	572991.0
40	506624.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.059347378833145	12.518391501382172	27.084460596062936	31.337800523721743
2	30.8338657948772	16.279210426734323	25.183854318521597	27.703069459866875
3	28.118304242803855	19.537269883007387	20.087271588439034	32.25715428574972
4	27.49089533472145	19.94599991627894	20.155535124860656	32.40756962413896
5	27.98857052491554	20.000108527300043	19.114293200454576	32.897027747329844
6	29.363223818951656	22.52406592878439	22.21972437166569	25.892985880598264
7	27.62856996677514	22.078406323110578	21.85328969502278	28.439734015091496
8	25.9046138056028	24.01244032936485	22.319181890204483	27.763763974827864
9	25.83476873607556	24.020502357367995	23.04491944948752	27.099809457068925
10	26.686010365907542	21.87251453103028	22.734453851866437	28.707021251195737
11	26.113218780184155	22.56856212180174	22.483290671768483	28.83492842624562
12	27.42779446169684	22.34879433921603	22.082592376112213	28.140818822974918
13	26.16849018370571	21.12879244774023	22.329569503208532	30.37314786534553
14	26.57771562436531	22.37956958072803	22.033134935092924	29.00957985981374
15	26.787483391447118	21.88840602853648	22.037631066094676	29.286479513921726
16	26.644382394391307	22.528484540286108	21.811274126006396	29.015858939316185
17	26.972677477019346	21.17483903075819	21.714297231468578	30.13818626075389
18	28.08601253645355	21.95592551306281	21.77243685649125	28.185625093992396
19	26.737250755427528	21.43553710935986	22.81840747039918	29.008804664813432
20	26.993220144527356	21.543211694901853	21.538095407899856	29.925472752670935
21	27.040429520045766	22.088018741114325	22.567941965801495	28.303609773038406
22	27.91981072838873	21.20452899926977	21.760266294986504	29.115393977355
23	27.01686359203658	22.48553873726936	21.600886202924347	28.89671146776972
24	27.230274775619804	23.266547700073954	21.567397778911285	27.935779745394957
25	26.83887881996716	21.548017903903727	21.867320724528255	29.745782551600858
26	27.103685432070435	21.954530162062266	22.48902711477072	28.452757291096574
27	27.069421813057076	22.669492510841103	21.593289291921383	28.667796384180438
28	26.98197981702297	22.094142781616714	21.75654535898505	29.16733204237526
29	27.083530362062575	23.56174195618908	21.4928240198822	27.861903661866144
30	28.19547007049623	23.154454503030237	21.54592487740291	27.10415054907062
31	27.064072967554985	22.60561644281619	22.62119786232227	27.709112727306557
32	27.39802697368523	22.94895030845009	21.334761759320557	28.318260958544123
33	27.39337580368342	24.436704553030314	21.47545965187543	26.69445999141084
34	27.520895381233153	23.249725968567393	21.376312211336764	27.853066438862694
35	28.67709872418407	23.678873920734762	20.931427800663258	26.712599554417913
36	27.987872849415268	24.4620534295402	21.755150007984508	25.79492371306002
37	28.834773387245562	24.409572728019732	20.550264419014603	26.205389465720103
38	29.159812650872325	24.547169840573396	20.333442377430043	25.959575131124236
39	28.584869400893613	25.186169786845603	21.018608971381898	25.210351840878882
40	28.18345454799155	26.37089359828465	19.794991930220046	25.650659923503756
41	28.41043164408007	25.58515594597821	20.53855897451004	25.465853435431683
42	29.69748790308202	26.312366375761826	19.18677393298284	24.803371788173315
43	28.449268913595215	27.244848441625493	19.114525758954663	25.19135688582463
44	28.81849429223921	26.487017809329938	19.554371402126204	25.140116496304643
45	28.775470969722434	27.19221270110496	18.38235408116912	25.649962248003483
46	28.766013590718746	27.175933606098614	19.23522362050174	24.822829182680902
47	29.892914562658234	27.356399002168995	17.902198297981858	24.84848813719091
48	29.91593785416722	27.976167404910708	17.562120251349228	24.545774489572853
49	29.708728230586406	27.94058595439683	17.516771343831543	24.833914471185224
50	29.845937745639915	28.076245079449734	17.29847643174641	24.779340743163942
51	29.561828778029113	28.49841627661438	16.974599960620093	24.965154984736408
52	29.402913802967134	28.562447383639356	17.001731785630675	25.032907027762835
53	29.355006751948455	28.546400847133096	16.8728168570804	25.225775543838054
54	29.11074280735319	29.02159538231842	16.85987110057535	25.007790709753035
55	28.836788894246347	28.734695712706532	16.83909587456725	25.589419518479872
56	28.692525104690088	28.80702140623474	16.668863052500857	25.83159043657432
57	28.362292034561293	28.99330076480739	16.90746807359391	25.736939127037406
58	28.162524282983387	28.733765478706168	16.744909682030514	26.35880055627993
59	27.845392008359703	28.75074224921279	16.842351693568517	26.561514048858992
60	27.797097359840865	28.78322291972546	16.864057153576983	26.55562256685669
61	27.845547047359766	28.398803719075534	17.02715818164059	26.728491051924113
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37.0
1	27.0
2	23.5
3	30.0
4	31.5
5	33.0
6	31.0
7	30.5
8	32.0
9	24.5
10	17.0
11	19.5
12	24.0
13	26.0
14	30.5
15	35.0
16	36.5
17	41.0
18	44.0
19	63.0
20	99.5
21	117.0
22	183.5
23	250.0
24	300.5
25	558.5
26	766.0
27	990.0
28	1214.0
29	1914.5
30	2985.5
31	3356.0
32	4157.5
33	4959.0
34	6048.5
35	8390.5
36	9643.0
37	11640.5
38	13638.0
39	16224.5
40	21904.5
41	24998.0
42	28550.5
43	35429.0
44	38755.0
45	42664.5
46	46574.0
47	50627.0
48	59613.5
49	64547.0
50	68381.5
51	72216.0
52	77375.0
53	87303.0
54	92072.0
55	96102.5
56	100133.0
57	102319.5
58	103536.5
59	102567.0
60	98986.5
61	94475.0
62	93544.0
63	79913.5
64	66283.0
65	58275.0
66	43271.5
67	36276.0
68	30920.5
69	25565.0
70	21112.5
71	13712.5
72	10765.0
73	8795.5
74	6826.0
75	5527.0
76	3306.0
77	2384.0
78	1837.5
79	1291.0
80	987.5
81	529.0
82	374.0
83	294.0
84	157.5
85	101.0
86	69.5
87	38.0
88	39.5
89	30.5
90	20.0
91	17.0
92	14.0
93	10.5
94	9.0
95	11.0
96	8.5
97	6.0
98	6.5
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.030232605011790717
3	1.5503900006046521E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.1889971922437086
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1289998.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.77736375052618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.98432993004883	61.30572061022412
2	13.490737631903555	20.176035903275285
3	2.695094771468004	6.045962462046125
4	0.7108858497132086	2.1263267907642596
5	0.276023276024728	1.0320146457456487
6	0.15697495007745244	0.704290376499741
7	0.09714213239392389	0.5084822798665554
8	0.07758733096545947	0.46414208560293013
9	0.05712262662127311	0.3844331486319987
>10	0.45049890182663893	6.088390546142549
>50	0.0032659088163606775	0.15226159185189278
>100	2.244600937049789E-4	0.02635171849689341
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	1.1223004685248945E-4	0.9855878408519625
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGTATGATCTGGGGGGGGGGGGGGG	11744	0.9103890083550517	TruSeq Adapter, Index 20 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.034420208403424	0.0	7.751950003023261E-5	0.0	0.0
2	1.036745793404331	0.0	7.751950003023261E-5	0.0	0.0
3	1.0379085859047843	0.0	7.751950003023261E-5	0.0	0.0
4	1.039458975905389	0.0	7.751950003023261E-5	0.0	0.0
5	1.0416295219062355	0.0	7.751950003023261E-5	0.0	0.0
6	1.046823328408261	0.0	7.751950003023261E-5	0.0	0.0
7	1.0513969789100448	0.0	1.5503900006046521E-4	0.0	0.0
8	1.1065908629315704	0.0	1.5503900006046521E-4	0.0	0.0
9	1.1131800204341402	0.0	1.5503900006046521E-4	0.0	0.0
10	1.1156606444351076	0.0	1.5503900006046521E-4	0.0	0.0
11	1.1226373994378285	0.0	1.5503900006046521E-4	0.0	0.0
12	1.136125792443089	0.0	1.5503900006046521E-4	0.0	0.0
13	1.1558932649507985	0.0	1.5503900006046521E-4	0.0	0.0
14	1.185118116462196	0.0	1.5503900006046521E-4	0.0	0.0
15	1.2203119694759217	0.0	1.5503900006046521E-4	0.0	0.0
16	1.2648081624932752	0.0	1.5503900006046521E-4	0.0	0.0
17	1.3031803150082404	0.0	1.5503900006046521E-4	0.0	0.0
18	1.3418625455233264	0.0	1.5503900006046521E-4	0.0	0.0
19	1.3948083640439752	0.0	1.5503900006046521E-4	0.0	0.0
20	1.4608549780697335	0.0	1.5503900006046521E-4	0.0	0.0
21	1.5564365216070102	0.0	1.5503900006046521E-4	0.0	0.0
22	1.6985297651624267	0.0	1.5503900006046521E-4	0.0	0.0
23	1.8841114482348034	0.0	1.5503900006046521E-4	0.0	7.751950003023261E-5
24	2.1410885908350243	0.0	1.5503900006046521E-4	0.0	7.751950003023261E-5
25	2.472484453464269	0.0	1.5503900006046521E-4	0.0	7.751950003023261E-5
26	2.8838029206246834	0.0	1.5503900006046521E-4	0.0	7.751950003023261E-5
27	3.408997533329509	0.0	1.5503900006046521E-4	0.0	7.751950003023261E-5
28	3.9962852655585515	0.0	1.5503900006046521E-4	0.0	7.751950003023261E-5
29	4.712642965337931	0.0	1.5503900006046521E-4	0.0	7.751950003023261E-5
30	5.554969852666439	0.0	1.5503900006046521E-4	0.0	7.751950003023261E-5
31	6.4903201400312245	0.0	1.5503900006046521E-4	0.0	1.5503900006046521E-4
32	7.597453639463007	0.0	1.5503900006046521E-4	0.0	1.5503900006046521E-4
33	8.841021458948	0.0	1.5503900006046521E-4	0.0	1.5503900006046521E-4
34	10.176682444468906	7.751950003023261E-5	1.5503900006046521E-4	0.0	1.5503900006046521E-4
35	11.663894052548919	7.751950003023261E-5	1.5503900006046521E-4	0.0	2.325585000906978E-4
36	13.270485690675489	7.751950003023261E-5	1.5503900006046521E-4	0.0	2.325585000906978E-4
37	14.986612382344779	7.751950003023261E-5	2.325585000906978E-4	0.0	2.325585000906978E-4
38	16.785452380546328	7.751950003023261E-5	2.325585000906978E-4	0.0	2.325585000906978E-4
39	18.722742205801868	7.751950003023261E-5	2.325585000906978E-4	0.0	2.325585000906978E-4
40	20.79251285660908	7.751950003023261E-5	2.325585000906978E-4	0.0	2.325585000906978E-4
41	22.98569455146442	7.751950003023261E-5	2.325585000906978E-4	0.0	2.325585000906978E-4
42	25.238798819843133	7.751950003023261E-5	2.325585000906978E-4	0.0	2.325585000906978E-4
43	27.549732635244396	7.751950003023261E-5	2.325585000906978E-4	0.0	2.325585000906978E-4
44	29.938961145676195	7.751950003023261E-5	2.325585000906978E-4	0.0	2.325585000906978E-4
45	32.33873230811211	7.751950003023261E-5	2.325585000906978E-4	0.0	2.325585000906978E-4
46	34.768270958559626	7.751950003023261E-5	2.325585000906978E-4	0.0	2.325585000906978E-4
47	37.22036778351595	7.751950003023261E-5	2.325585000906978E-4	0.0	2.325585000906978E-4
48	39.61517769794992	7.751950003023261E-5	2.325585000906978E-4	0.0	2.325585000906978E-4
49	42.08107299391162	7.751950003023261E-5	2.325585000906978E-4	0.0	2.325585000906978E-4
50	44.47448755734505	7.751950003023261E-5	2.325585000906978E-4	0.0	2.325585000906978E-4
>>END_MODULE