##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-33B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 777185 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.57136074422435 40.0 40.0 40.0 40.0 40.0 2 39.36748907917677 40.0 40.0 40.0 40.0 40.0 3 39.31555035158939 40.0 40.0 40.0 40.0 40.0 4 39.21365440660846 40.0 40.0 40.0 40.0 40.0 5 39.253644885065974 40.0 40.0 40.0 40.0 40.0 6 39.37260755161255 40.0 40.0 40.0 40.0 40.0 7 39.38726043348752 40.0 40.0 40.0 40.0 40.0 8 39.397626047852185 40.0 40.0 40.0 40.0 40.0 9 39.39309173491511 40.0 40.0 40.0 40.0 40.0 10-11 39.396390820718366 40.0 40.0 40.0 40.0 40.0 12-13 39.30990176084201 40.0 40.0 40.0 40.0 40.0 14-15 39.34715929926594 40.0 40.0 40.0 40.0 40.0 16-17 39.45620412128386 40.0 40.0 40.0 40.0 40.0 18-19 39.49343849919903 40.0 40.0 40.0 40.0 40.0 20-21 39.46856153940182 40.0 40.0 40.0 40.0 40.0 22-23 39.50730392377619 40.0 40.0 40.0 40.0 40.0 24-25 39.49091400374428 40.0 40.0 40.0 40.0 40.0 26-27 39.468777704150234 40.0 40.0 40.0 40.0 40.0 28-29 39.48524739926787 40.0 40.0 40.0 40.0 40.0 30-31 39.45172129373327 40.0 40.0 40.0 40.0 40.0 32-33 39.45164900945154 40.0 40.0 40.0 40.0 40.0 34-35 39.443250547698355 40.0 40.0 40.0 40.0 40.0 36-37 39.4303045609127 40.0 40.0 40.0 40.0 40.0 38-39 39.36917179428645 40.0 40.0 40.0 40.0 40.0 40-41 39.413266923785955 40.0 40.0 40.0 40.0 40.0 42-43 39.38959692765121 40.0 40.0 40.0 40.0 40.0 44-45 39.29976136517266 40.0 40.0 40.0 40.0 40.0 46-47 39.26659069728329 40.0 40.0 40.0 40.0 40.0 48-49 39.33976455023223 40.0 40.0 40.0 40.0 40.0 50-51 39.282345046830855 40.0 40.0 40.0 40.0 40.0 52-53 39.230399599647846 40.0 40.0 40.0 40.0 40.0 54-55 39.13289091994611 40.0 40.0 40.0 40.0 40.0 56-57 39.159803263484775 40.0 40.0 40.0 40.0 40.0 58-59 39.10309970955096 40.0 40.0 40.0 40.0 40.0 60-61 39.010820870472244 40.0 40.0 40.0 40.0 40.0 62-63 39.36441935061222 40.0 40.0 40.0 40.0 40.0 64-65 39.370801188460334 40.0 40.0 40.0 40.0 40.0 66-67 39.378190702909954 40.0 40.0 40.0 40.0 40.0 68-69 39.372427137830925 40.0 40.0 40.0 40.0 40.0 70-71 39.3502294549521 40.0 40.0 40.0 40.0 40.0 72-73 39.35223496748759 40.0 40.0 40.0 40.0 40.0 74-75 39.360909759289854 40.0 40.0 40.0 40.0 40.0 76-77 39.35631351669605 40.0 40.0 40.0 40.0 40.0 78-79 39.36008318792984 40.0 40.0 40.0 40.0 40.0 80-81 39.337959899709205 40.0 40.0 40.0 40.0 40.0 82-83 39.301967682927994 40.0 40.0 40.0 40.0 40.0 84-85 39.31670953566733 40.0 40.0 40.0 40.0 40.0 86-87 39.28584930007564 40.0 40.0 40.0 40.0 40.0 88-89 39.34006896769904 40.0 40.0 40.0 40.0 40.0 90-91 39.39326620980296 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.011100559065553739 1101 2 0.0661165270247821 1101 3 0.041274235477239074 1101 4 -0.13209616022935222 1101 5 -0.06324502315781899 1101 6 0.14898144342797792 1101 7 0.0502437982640771 1101 8 0.02579439924781468 1101 9 -0.009116275469473578 1101 10-11 -0.030616715698556618 1101 12-13 -0.05323179111576337 1101 14-15 0.022680313633891558 1101 16-17 -0.011511664485183815 1101 18-19 -0.009913220683060331 1101 20-21 -0.03729164566180998 1101 22-23 0.03024578526422772 1101 24-25 -0.016284399300474206 1101 26-27 0.022258573201369813 1101 28-29 0.10508528317417642 1101 30-31 -0.031756279692217504 1101 32-33 0.05003127804031493 1101 34-35 -8.950961763076748E-5 1101 36-37 -0.0595568927356922 1101 38-39 0.03838297160381643 1101 40-41 -0.0059552363963462085 1101 42-43 0.008916427379041636 1101 44-45 -0.08421611442529553 1101 46-47 0.04684892878785263 1101 48-49 0.013851719123088913 1101 50-51 -0.03331775770461576 1101 52-53 0.10340330065240977 1101 54-55 -0.00316086114175107 1101 56-57 0.15057745257900024 1101 58-59 0.04052812539887185 1101 60-61 0.25585972726240414 1101 62-63 0.06140031521120903 1101 64-65 -0.005189489577333006 1101 66-67 0.05449948145579242 1101 68-69 -0.051051196416416644 1101 70-71 0.11048247002464251 1101 72-73 -0.02120237477173248 1101 74-75 -0.1024466279209193 1101 76-77 0.17955422474077665 1101 78-79 0.2450663847526826 1101 80-81 0.30797790551506665 1101 82-83 0.34665270371351653 1101 84-85 -0.325119651291061 1101 86-87 -0.01892112128881962 1101 88-89 -0.10928574372661615 1101 90-91 0.4072805819588865 1102 1 -0.04215409658134206 1102 2 -0.015020823113680137 1102 3 -0.03020402543187828 1102 4 -0.14849528069143503 1102 5 -0.09419486088811624 1102 6 -0.00539115126573364 1102 7 -0.05278011931824267 1102 8 -0.18883049975291755 1102 9 -0.0687127241156773 1102 10-11 -0.05117701746493708 1102 12-13 -0.11046861481862891 1102 14-15 -0.019108425797426776 1102 16-17 -0.08112196478475653 1102 18-19 -0.058853151900081 1102 20-21 -0.12050935904061078 1102 22-23 -0.02014685259678828 1102 24-25 -0.01952547242041902 1102 26-27 0.008721657783326009 1102 28-29 -0.07615965549172898 1102 30-31 -2.1871216489444123E-5 1102 32-33 -0.06758217053300086 1102 34-35 -0.10162108499401512 1102 36-37 -0.18761333081553744 1102 38-39 0.10275333165223088 1102 40-41 -0.09125446545527893 1102 42-43 0.049712635822025675 1102 44-45 -0.07748523520361772 1102 46-47 0.03240582492232136 1102 48-49 -0.0613843826956284 1102 50-51 0.05850853754272123 1102 52-53 -0.05408724204097126 1102 54-55 0.028063139208455823 1102 56-57 -0.10494254563680272 1102 58-59 0.036744934508405436 1102 60-61 -0.04226984613467977 1102 62-63 -0.14578652417119287 1102 64-65 0.06730446561712711 1102 66-67 -0.0385084168058043 1102 68-69 0.19278104378764738 1102 70-71 -0.228210572142018 1102 72-73 0.0056822283171484855 1102 74-75 0.137755559189344 1102 76-77 -0.28726407950724564 1102 78-79 -0.07200143462452502 1102 80-81 0.12146659409857108 1102 82-83 0.0893024685877748 1102 84-85 0.3314623269122592 1102 86-87 -0.10400270637040876 1102 88-89 -0.03674495118582399 1102 90-91 0.4072805819588865 1103 1 0.05896571033625975 1103 2 0.15796520580590823 1103 3 0.002974730954036886 1103 4 -0.04136076052385107 1103 5 -0.037048259419520946 1103 6 0.10125647654464842 1103 7 0.068975732832115 1103 8 -0.1055427841380947 1103 9 -0.0037121393898118527 1103 10-11 -0.02712453501922596 1103 12-13 -0.20744483126458135 1103 14-15 0.04990697319720283 1103 16-17 -0.0516654322137029 1103 18-19 -0.011103572925463823 1103 20-21 0.005166204105002237 1103 22-23 -0.06398900664498086 1103 24-25 0.0223964145741391 1103 26-27 -0.008374179469043952 1103 28-29 -0.05465493968897306 1103 30-31 -0.06622376044983724 1103 32-33 -0.06737776717550759 1103 34-35 -0.011200584085990783 1103 36-37 -0.009508353785044221 1103 38-39 0.009958502246952605 1103 40-41 0.03988838408443485 1103 42-43 0.09720461244701539 1103 44-45 0.01081720286283172 1103 46-47 -0.02040540886423514 1103 48-49 0.18772600999472644 1103 50-51 -0.10324851016600434 1103 52-53 0.06517878838049995 1103 54-55 0.03185756776505855 1103 56-57 0.041220061689685394 1103 58-59 0.227401576762702 1103 60-61 0.3801357817272475 1103 62-63 0.24565049387770443 1103 64-65 0.049979718182243005 1103 66-67 0.03998758458038054 1103 68-69 -0.2223765868456269 1103 70-71 -0.22056077026660148 1103 72-73 -0.21797114839765896 1103 74-75 -0.029508319425048057 1103 76-77 0.06647214793020595 1103 78-79 0.23956927243138182 1103 80-81 0.20173751321521394 1103 82-83 0.4727396602352485 1103 84-85 0.41635982463379406 1103 86-87 0.767792165424467 1103 88-89 0.6832550488141749 1103 90-91 0.4072805819588865 1104 1 -0.022062686481604032 1104 2 -0.09171497369883497 1104 3 -0.09276203144939643 1104 4 -0.04588350188870294 1104 5 -0.08711452001210063 1104 6 -0.017439817590243933 1104 7 -0.053548951096075825 1104 8 -0.024849405199105945 1104 9 -0.02370244008186262 1104 10-11 -0.06035074368403315 1104 12-13 -0.0306877773145402 1104 14-15 -0.038333764166338824 1104 16-17 -0.020460137850520255 1104 18-19 -0.06443490695855303 1104 20-21 0.07137005978254507 1104 22-23 -0.026218442558622712 1104 24-25 0.05060816208412433 1104 26-27 0.03412445952082521 1104 28-29 -0.029184124744467965 1104 30-31 -0.03404313177798457 1104 32-33 -0.04404409901841433 1104 34-35 -0.048423630065379086 1104 36-37 -0.0896657305160602 1104 38-39 0.011006989411619372 1104 40-41 -0.06988938051561888 1104 42-43 -0.061942586311594994 1104 44-45 0.0013410460467966345 1104 46-47 -0.055158433768284 1104 48-49 -0.0521186557886395 1104 50-51 0.09263577851206861 1104 52-53 0.0373009006460876 1104 54-55 0.1015625272568812 1104 56-57 0.13515214881672932 1104 58-59 0.00858162733040757 1104 60-61 -0.08823840438130048 1104 62-63 0.06308313675490496 1104 64-65 0.09776315399980717 1104 66-67 0.11100733746066993 1104 68-69 0.2737083364890651 1104 70-71 -0.2924565628858531 1104 72-73 0.1680010934075682 1104 74-75 -0.05171596656771271 1104 76-77 -0.08754038102195949 1104 78-79 -0.15451541187534445 1104 80-81 -0.3716990828240583 1104 82-83 -0.1271568200960118 1104 84-85 0.10468367876561047 1104 86-87 -0.30293813934839164 1104 88-89 -0.4596020940429639 1104 90-91 0.4072805819588865 1201 1 -0.03595127561520428 1201 2 0.09578751972017585 1201 3 0.012214669389372546 1201 4 0.019611830103912098 1201 5 -0.03730761692025908 1201 6 0.06006055029124724 1201 7 0.10607695430115172 1201 8 -0.021775550945577038 1201 9 0.12793406030236554 1201 10-11 -0.042620490745065354 1201 12-13 0.043568693312167284 1201 14-15 0.029542169843892907 1201 16-17 0.01623121775190839 1201 18-19 -5.026625544388708E-4 1201 20-21 0.0021089293235974083 1201 22-23 -0.05840889072003819 1201 24-25 -0.0031358904983989078 1201 26-27 0.022651789028195424 1201 28-29 -0.041085826809514 1201 30-31 0.0822754372447605 1201 32-33 -4.3951249080720345E-4 1201 34-35 0.05290112387442747 1201 36-37 -0.0022905523492227076 1201 38-39 -0.003701476333553444 1201 40-41 0.08151156381946123 1201 42-43 0.13029618873905946 1201 44-45 0.045313506010266735 1201 46-47 -0.035440570072466926 1201 48-49 0.21527188551478815 1201 50-51 0.09173076041451367 1201 52-53 0.0278608623913712 1201 54-55 -0.009738442787295298 1201 56-57 0.11511883149198354 1201 58-59 0.29611461213418977 1201 60-61 0.24467669616961274 1201 62-63 0.13262918381025912 1201 64-65 0.11575579796217994 1201 66-67 0.09423843994101588 1201 68-69 -0.03193490958704359 1201 70-71 0.11806728089214147 1201 72-73 0.24535593007870915 1201 74-75 -0.14137730496641865 1201 76-77 -0.0742381292215768 1201 78-79 0.04257795277527521 1201 80-81 0.01752029884792705 1201 82-83 0.031342470030999436 1201 84-85 0.2762488622160362 1201 86-87 0.5455699432022456 1201 88-89 0.6832550488141749 1201 90-91 -1.814941640263335 1202 1 0.029713083534154805 1202 2 -0.13251672589500174 1202 3 -0.02946970991013842 1202 4 -0.0691043019337485 1202 5 0.06514528480983728 1202 6 0.04324709085697265 1202 7 0.06733059703680766 1202 8 0.026238142490456084 1202 9 0.10647436602132387 1202 10-11 0.04463399699692161 1202 12-13 -0.06662013310403125 1202 14-15 -0.0367401272733332 1202 16-17 0.09803476461185312 1202 18-19 0.04645022292553591 1202 20-21 0.06391312618840317 1202 22-23 -0.011753707472827557 1202 24-25 0.08554080791017071 1202 26-27 0.041904500090197416 1202 28-29 0.044856084421489584 1202 30-31 0.04591936457421042 1202 32-33 -0.07496928997684904 1202 34-35 0.005211995697983696 1202 36-37 -0.2319252894701549 1202 38-39 -0.08116054832798625 1202 40-41 -0.08275881417112174 1202 42-43 -0.10260754887232792 1202 44-45 -0.08336539692864875 1202 46-47 -0.061978665229524665 1202 48-49 0.06731929506353396 1202 50-51 0.041740712206646435 1202 52-53 0.04218056494700306 1202 54-55 0.08579915990799947 1202 56-57 -0.018035297585072385 1202 58-59 -0.14041500620353986 1202 60-61 -0.038128042298808396 1202 62-63 -0.28958468811431004 1202 64-65 -0.28820168919037314 1202 66-67 -0.1523399078955947 1202 68-69 -0.44927741208723404 1202 70-71 -0.2137236026652971 1202 72-73 0.020399286182502863 1202 74-75 0.12701820937089536 1202 76-77 0.28006413041750733 1202 78-79 -0.061774350120202826 1202 80-81 -0.12186893510652652 1202 82-83 0.07012796263180832 1202 84-85 -0.23986138623116915 1202 86-87 0.43907099142027306 1202 88-89 -0.4683933028341727 1202 90-91 0.4072805819588865 1203 1 0.11241156125046814 1203 2 0.05145817946765874 1203 3 0.08868523860547128 1203 4 -0.08535801971098778 1203 5 -0.04188074526467034 1203 6 0.09329850234194481 1203 7 0.03257581457565806 1203 8 0.04993824054124474 1203 9 -0.024075898150194064 1203 10-11 0.011006422942955396 1203 12-13 0.023697053885740615 1203 14-15 -0.0020843235198242382 1203 16-17 -0.10121644379002959 1203 18-19 -0.031864028285070845 1203 20-21 -0.013298219422708257 1203 22-23 0.04521074812439707 1203 24-25 0.023093638900057556 1203 26-27 -0.015562802691420075 1203 28-29 0.054797258370818724 1203 30-31 -0.04708615481434464 1203 32-33 0.07145139927239796 1203 34-35 -0.019736977753517237 1203 36-37 0.06342347610653576 1203 38-39 -0.02620851825034265 1203 40-41 0.010453542998590137 1203 42-43 -0.028189739097427946 1203 44-45 -0.037163435063938266 1203 46-47 0.09735080891616832 1203 48-49 0.009190064263172815 1203 50-51 0.013326916633239705 1203 52-53 0.049599505967904634 1203 54-55 0.08097316636902008 1203 56-57 0.0024853013870824725 1203 58-59 0.10834324703459686 1203 60-61 -0.0607292518790814 1203 62-63 0.11397361444052478 1203 64-65 6.446182363433195E-4 1203 66-67 -0.21981231674727297 1203 68-69 0.06335458802998772 1203 70-71 -0.07078073792105499 1203 72-73 -0.04813796442980589 1203 74-75 0.08627347740289792 1203 76-77 -0.3004974478283273 1203 78-79 0.19900899798709304 1203 80-81 0.1405405556076147 1203 82-83 0.009963228314362027 1203 84-85 0.0682269001618181 1203 86-87 -0.9285041308718291 1203 88-89 -1.0691259035667784 1203 90-91 -2.3927194180411107 1204 1 0.007890859261415528 1204 2 -0.15228257110853605 1204 3 -0.19880373749109737 1204 4 -0.15138407054716652 1204 5 -0.285377707748971 1204 6 -0.13291429095945517 1204 7 -0.176832779643064 1204 8 -0.14826056018920752 1204 9 -0.22192849093949718 1204 10-11 -0.21778325901128426 1204 12-13 -0.24094219809528283 1204 14-15 -0.15582556586866758 1204 16-17 -0.1507071312554018 1204 18-19 -0.19207568723091129 1204 20-21 -0.1630803641415639 1204 22-23 -0.041818584474562215 1204 24-25 -0.13448867564428468 1204 26-27 -0.10385894162046583 1204 28-29 -0.07407995911398757 1204 30-31 -0.03531639620914717 1204 32-33 -0.18453650043435488 1204 34-35 -0.031126802793771446 1204 36-37 -0.13082025653197604 1204 38-39 0.0287201960361827 1204 40-41 -0.17296656022118384 1204 42-43 -0.06576999298086861 1204 44-45 -0.05949390124359155 1204 46-47 -0.10700391988262226 1204 48-49 0.024976010658328107 1204 50-51 -0.1512924243751783 1204 52-53 -0.11600463455847176 1204 54-55 -0.19178374622943295 1204 56-57 -0.22370255483195933 1204 58-59 -0.008011308414268115 1204 60-61 -0.22956786424710174 1204 62-63 -0.16875891268914955 1204 64-65 -0.10844371267508279 1204 66-67 -0.16014255509709585 1204 68-69 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2401 32-33 0.029124626379854135 2401 34-35 -0.08608932123495805 2401 36-37 0.027470025910034224 2401 38-39 0.12703255055564 2401 40-41 0.018095483267600798 2401 42-43 0.07346932247742899 2401 44-45 0.09302505469828048 2401 46-47 0.08918598538390654 2401 48-49 0.1560333634564799 2401 50-51 0.11055100628824022 2401 52-53 -0.01973318943770863 2401 54-55 -0.03976305432987459 2401 56-57 0.009758882303742666 2401 58-59 0.02988820625671451 2401 60-61 0.19858894645956582 2401 62-63 0.18025001783248484 2401 64-65 0.05456506842883613 2401 66-67 0.0338009071290557 2401 68-69 0.08926788654859052 2401 70-71 0.06018287392717525 2401 72-73 0.12573707436076376 2401 74-75 0.09735087555854705 2401 76-77 0.2513873924775609 2401 78-79 0.31310736083217705 2401 80-81 -0.0031205539779364244 2401 82-83 0.1876298510736376 2401 84-85 0.3121192344613206 2401 86-87 0.38683978447208744 2401 88-89 -1.016744951185828 2401 90-91 -0.0927194180411135 2402 1 0.007632784003334336 2402 2 9.308821049174298E-4 2402 3 0.08601977233241342 2402 4 0.10744876328566733 2402 5 0.10702988465862262 2402 6 0.07534411729895396 2402 7 -0.01734227015255385 2402 8 0.03399052206187747 2402 9 0.07817973416872803 2402 10-11 0.09012549889747135 2402 12-13 0.10473296007987898 2402 14-15 0.07413197658887327 2402 16-17 0.04408141353314221 2402 18-19 0.09243563478041494 2402 20-21 0.04647674541554636 2402 22-23 0.01412963397365985 2402 24-25 0.0596963060406992 2402 26-27 -0.01736210512363101 2402 28-29 0.06057705054301721 2402 30-31 0.09178566839292301 2402 32-33 0.064998224931891 2402 34-35 0.10328612653187719 2402 36-37 0.002882414555465118 2402 38-39 -0.3397179393321892 2402 40-41 0.1132690311165021 2402 42-43 -0.00574264878979136 2402 44-45 0.09138751357792074 2402 46-47 0.19026013140670273 2402 48-49 0.05480361375592224 2402 50-51 0.18357918924289862 2402 52-53 0.08583847891537744 2402 54-55 -0.006133132962062859 2402 56-57 0.09715364728410947 2402 58-59 -0.164363982642854 2402 60-61 0.10155316463691122 2402 62-63 0.19909801989820863 2402 64-65 0.1390507468464648 2402 66-67 0.12138256864252384 2402 68-69 0.02262177127878573 2402 70-71 0.1768319844424795 2402 72-73 0.276649649953022 2402 74-75 0.36672081330697637 2402 76-77 0.19397762196434343 2402 78-79 0.10034257929549995 2402 80-81 0.2729304533239585 2402 82-83 0.2954622275230392 2402 84-85 0.3482601425049623 2402 86-87 -0.061145824596920306 2402 88-89 0.4479609311671169 2402 90-91 0.4072805819588865 2403 1 8.624325204564798E-4 2403 2 -0.0544015335971082 2403 3 0.19626621651109133 2403 4 0.18640736457486895 2403 5 0.17499477397874585 2403 6 0.03353172025041573 2403 7 0.07649117224351443 2403 8 0.14004087143871402 2403 9 0.09642611817486824 2403 10-11 0.013892525847253978 2403 12-13 0.1636470524898641 2403 14-15 0.08100810012311399 2403 16-17 -0.007567314193607899 2403 18-19 0.12119858127950067 2403 20-21 0.04065881249289305 2403 22-23 0.0691419129335813 2403 24-25 0.09755470876049088 2403 26-27 0.03860024190647948 2403 28-29 0.06898710387093132 2403 30-31 0.055047039123493846 2403 32-33 0.08993588247160034 2403 34-35 0.10099073123911495 2403 36-37 0.09016786691205425 2403 38-39 0.099042164744084 2403 40-41 0.09385618455175404 2403 42-43 0.10278233932894665 2403 44-45 0.16659139895674713 2403 46-47 0.03415885643030947 2403 48-49 0.11212514482574676 2403 50-51 0.1116159891550339 2403 52-53 0.23345708803709186 2403 54-55 0.03534620895095486 2403 56-57 0.14662883334609944 2403 58-59 0.1420315687538931 2403 60-61 0.23791021674199442 2403 62-63 0.22703695623442854 2403 64-65 0.24101662324636663 2403 66-67 0.2516332210913177 2403 68-69 0.17740933634390643 2403 70-71 0.33370125110270976 2403 72-73 0.4365562037844697 2403 74-75 0.05154866853440865 2403 76-77 0.26648757848683857 2403 78-79 0.08764732570752187 2403 80-81 -0.021872722411480083 2403 82-83 0.37561347041012993 2403 84-85 0.008348429315603312 2403 86-87 0.26476186239416677 2403 88-89 0.3354289618576516 2403 90-91 0.4072805819588865 2404 1 0.062166901477155534 2404 2 -0.09349914348089072 2404 3 -0.15625064569177738 2404 4 0.042133634528326525 2404 5 -0.02156915449318575 2404 6 -0.008234950199707214 2404 7 -0.06386701843609899 2404 8 0.07650897513309474 2404 9 0.0858200419346673 2404 10-11 0.02456601650382595 2404 12-13 -0.01356756268044279 2404 14-15 0.06959992990859121 2404 16-17 0.027030039446593435 2404 18-19 0.042027228279039264 2404 20-21 0.06205463486278262 2404 22-23 0.07397717755235789 2404 24-25 -0.014091819569038933 2404 26-27 0.05778377077601249 2404 28-29 0.06037319242458494 2404 30-31 0.05759571680346909 2404 32-33 0.03773685847310304 2404 34-35 0.10651697653448622 2404 36-37 0.0637968877878663 2404 38-39 0.08235852951428058 2404 40-41 0.0714093302708676 2404 42-43 0.12540178015045456 2404 44-45 0.10527059896616464 2404 46-47 0.09089360586570194 2404 48-49 0.06886297799977825 2404 50-51 0.13851081313764269 2404 52-53 0.003012482267969574 2404 54-55 0.17005993139206765 2404 56-57 0.006394367543514079 2404 58-59 0.12753232350237909 2404 60-61 0.1177641588434426 2404 62-63 -0.036660694601650334 2404 64-65 0.23019901046215807 2404 66-67 0.13520777074476342 2404 68-69 0.06496556158496958 2404 70-71 0.2054634767677328 2404 72-73 0.27891141043696877 2404 74-75 0.21694158676066877 2404 76-77 -0.007271329935242932 2404 78-79 0.0583517209917801 2404 80-81 0.013836337500649165 2404 82-83 0.1949961790346748 2404 84-85 0.0695922559115445 2404 86-87 0.06603777945955613 2404 88-89 0.44083080638993266 2404 90-91 0.4072805819588865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 34.0 31 509.0 32 3142.0 33 5920.0 34 7793.0 35 9681.0 36 13922.0 37 20535.0 38 54164.0 39 323680.0 40 337805.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.675302534145665 11.349421308954753 26.91199650018979 32.063279656709796 2 30.644202409936227 16.214953752078195 24.891521986421587 28.249321851563984 3 28.82415383724596 18.483501354246414 19.736999556090247 32.95534525241738 4 27.854371867702028 19.813300565502423 19.202892490205034 33.12943507659051 5 28.483565688992968 19.98481699981343 19.14370452337603 32.38791278781758 6 28.741162014192245 22.599767108217478 22.241808578395105 26.417262299195173 7 27.015575442140545 21.976363414116328 22.034522025000484 28.973539118742643 8 26.40169329052928 22.85569073000637 22.296621782458487 28.44599419700586 9 26.23416561050458 22.950134137946563 22.956438943108783 27.859261308440075 10-11 26.298693361297502 21.675662808726365 22.5322799590831 29.49336387089303 12-13 26.694094713613875 21.571247515070414 22.240972226689912 29.493685544625798 14-15 27.136524765660685 21.39394095356961 22.01535027052761 29.45418401024209 16-17 27.370188565142143 21.1158861789664 21.644975134620456 29.868950121271 18-19 27.42371507427446 21.3661483430586 21.629341791208017 29.58079479145892 20-21 27.54003229604277 21.426043992099693 21.421604894587517 29.612318817270015 22-23 27.435552667640266 20.980139863739005 21.54512760796979 30.03917986065094 24-25 27.37591435758539 21.408609275783757 21.74881141555743 29.466664951073422 26-27 27.540997317241068 21.600455490005597 21.638219986232365 29.22032720652097 28-29 27.58950571614223 21.217856752253326 21.715293012603176 29.47734451900127 30-31 27.608218451886184 21.425014604119006 21.890802364451904 29.075964579542912 32-33 27.680215280922805 21.380001196297265 21.86890553918498 29.07087798359495 34-35 27.590060903394686 21.12333532648587 21.981071173232166 29.30553259688728 36-37 27.574961114454073 21.477735896184107 22.09465366318873 28.852649326173097 38-39 27.92791716923224 21.297335705840904 21.995802333496542 28.778944791430312 40-41 27.687549804576328 21.145837286077487 22.121927927244208 29.044684982101977 42-43 27.630472394292156 21.366056343147026 22.288007451339357 28.715463811221465 44-45 27.865263134876272 21.307824844626552 22.211865510967026 28.615046509530153 46-47 27.71840482352021 21.002179814384 22.376918726893326 28.902496635202468 48-49 27.7493069611779 21.24303573229024 22.44968760401003 28.557969702521824 50-51 27.913225315583457 21.21586961306932 22.29887835083119 28.572026720516035 52-53 27.865556142173027 21.014499499306478 22.323217949962565 28.796726408557937 54-55 27.757390721572715 21.26308734827612 22.490580087869535 28.48894184228163 56-57 27.97032941594844 21.213427583175935 22.438798549348647 28.377444451526973 58-59 27.84898022079204 20.86452469594961 22.48194150894221 28.804553574316138 60-61 28.12999154629004 21.156441444476076 22.48252211764348 28.231044891590408 62-63 28.146026663998597 20.96700767903149 22.464293754220964 28.42267190274894 64-65 27.940706849601405 20.812116426385803 22.413182448948785 28.83399427506401 66-67 28.249995755301626 20.956924800923645 22.406234612968404 28.386844830806325 68-69 28.18928065669779 20.891838481786397 22.43157397851011 28.487306883005704 70-71 28.190144993633965 20.719552287022786 22.252627178230053 28.8376755411132 72-73 28.451336019449613 20.684046523838333 22.391594662770657 28.4730227939414 74-75 28.51001675227402 20.781126694264078 22.240374364867296 28.468482188594606 76-77 28.26362161105872 20.506546359782156 22.156517897348323 29.073314131810807 78-79 28.757410205742183 20.59218243107584 21.943719421343506 28.70668794183847 80-81 28.74652633274712 20.22312019224315 22.02204352320477 29.008309951804968 82-83 28.856178544291495 19.780530473043417 21.8656676856744 29.49762329699069 84-85 29.571909106308837 19.79769135832345 20.81891440602086 29.811485129346853 86-87 30.403034277409517 19.30807131969799 20.481372514267484 29.807521888625004 88-89 29.54491585683811 18.52334676463617 20.893576676937663 31.03816070158805 90-91 25.530379564150675 20.637898686679172 23.25010824072738 30.58161350844278 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.0 10 1.0 11 1.0 12 2.0 13 4.5 14 8.0 15 11.5 16 17.5 17 30.5 18 47.0 19 66.5 20 101.0 21 151.5 22 215.5 23 311.5 24 457.5 25 629.0 26 829.0 27 1111.5 28 1482.5 29 1906.0 30 2370.5 31 2951.0 32 3656.0 33 4359.0 34 5242.5 35 6354.5 36 7676.5 37 9118.5 38 10677.5 39 12597.0 40 14741.0 41 17010.5 42 19328.5 43 21817.5 44 24401.0 45 26817.5 46 29548.5 47 32788.5 48 35667.0 49 38304.5 50 41258.0 51 44462.0 52 47352.0 53 49820.5 54 52442.5 55 54922.0 56 56945.5 57 58701.0 58 60209.5 59 61326.0 60 61975.0 61 62067.0 62 61574.0 63 60201.5 64 57816.0 65 54616.0 66 50589.5 67 46627.0 68 42176.0 69 37040.0 70 32162.5 71 27367.5 72 22881.5 73 18603.0 74 14669.5 75 11779.0 76 9372.0 77 6979.5 78 5232.0 79 3914.0 80 2836.5 81 2031.5 82 1425.5 83 1000.5 84 711.0 85 510.5 86 356.0 87 248.0 88 170.0 89 109.5 90 75.0 91 51.5 92 35.5 93 23.5 94 16.0 95 11.0 96 4.5 97 2.0 98 2.5 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0088781950243507 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06161337532391778 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.501306932872426E-4 64-65 2.9031031269323784E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 8.496176720475786E-4 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.005925223677193814 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 10597.0 32-33 24548.0 34-35 29263.0 36-37 33385.0 38-39 37001.0 40-41 40302.0 42-43 42447.0 44-45 43808.0 46-47 43751.0 48-49 43369.0 50-51 42489.0 52-53 40797.0 54-55 38284.0 56-57 35845.0 58-59 33337.0 60-61 30860.0 62-63 28322.0 64-65 25647.0 66-67 22915.0 68-69 20260.0 70-71 18232.0 72-73 15877.0 74-75 13910.0 76-77 11949.0 78-79 10336.0 80-81 9321.0 82-83 7871.0 84-85 6828.0 86-87 5880.0 88-89 5094.0 90-91 4660.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.93053852201375 #Duplication Level Percentage of deduplicated Percentage of total 1 99.93666629330592 99.8672488078484 2 0.05828289041170978 0.11648481250923338 3 0.0041842449228448216 0.012544015453436547 4 6.079052022593396E-4 0.00242993176928438 5 2.586661572653388E-4 0.0012924324196472601 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0015440339172783828 0.0 1.286694931065319E-4 0.0 0.0 2 0.0015440339172783828 0.0 1.286694931065319E-4 0.0 0.0 3 0.0015440339172783828 0.0 1.286694931065319E-4 0.0 0.0 4 0.0015440339172783828 0.0 1.286694931065319E-4 0.0 0.0 5 0.0015440339172783828 0.0 1.286694931065319E-4 0.0 0.0 6 0.0016727034103849148 0.0 1.286694931065319E-4 0.0 0.0 7 0.0020587118897045105 0.0 2.573389862130638E-4 0.0 0.0 8 0.00952154248988336 0.0 2.573389862130638E-4 0.0 0.0 9 0.010293559448522553 0.0 2.573389862130638E-4 0.0 0.0 10-11 0.010422228941629084 0.0 2.573389862130638E-4 0.0 0.0 12-13 0.010422228941629084 0.0 2.573389862130638E-4 0.0 0.0 14-15 0.010422228941629084 0.0 2.573389862130638E-4 0.0 0.0 16-17 0.010422228941629084 0.0 2.573389862130638E-4 0.0 0.0 18-19 0.010422228941629084 0.0 2.573389862130638E-4 0.0 0.0 20-21 0.01048656368818235 0.0 2.573389862130638E-4 0.0 0.0 22-23 0.010550898434735617 0.0 2.573389862130638E-4 0.0 6.433474655326595E-5 24-25 0.01080823742094868 0.0 2.573389862130638E-4 0.0 1.286694931065319E-4 26-27 0.010872572167501947 0.0 2.573389862130638E-4 0.0 1.286694931065319E-4 28-29 0.011322915393374808 0.0 2.573389862130638E-4 0.0 1.286694931065319E-4 30-31 0.011580254379587871 0.0 2.573389862130638E-4 0.0 1.9300423965979785E-4 32-33 0.011708923872694402 0.0 2.573389862130638E-4 0.0 2.573389862130638E-4 34-35 0.011901928112354201 1.286694931065319E-4 2.573389862130638E-4 0.0 3.216737327663298E-4 36-37 0.011966262858907468 1.286694931065319E-4 3.216737327663298E-4 0.0 3.860084793195957E-4 38-39 0.012545275577886861 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 40-41 0.013188623043419521 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 42-43 0.013767635762398915 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 44-45 0.014024974748611977 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 46-47 0.01415364424171851 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 48-49 0.01447531797448484 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 50-51 0.014668322214144637 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 52-53 0.014925661200357702 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 54-55 0.015183000186570764 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 56-57 0.015183000186570764 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 58-59 0.01556900866589036 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 60-61 0.015826347652103424 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 62-63 0.016019351891763223 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 64-65 0.016148021384869753 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 66-67 0.01634102562452955 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 68-69 0.01634102562452955 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 70-71 0.01634102562452955 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 72-73 0.01634102562452955 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 74-75 0.01634102562452955 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 76-77 0.01634102562452955 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 78-79 0.01634102562452955 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 80 0.01634102562452955 1.286694931065319E-4 3.860084793195957E-4 0.0 3.860084793195957E-4 >>END_MODULE