##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-33A_S38_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1225593
Sequences flagged as poor quality	0
Sequence length	61
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.42443209124073	40.0	40.0	40.0	40.0	40.0
2	39.3517064800468	40.0	40.0	40.0	40.0	40.0
3	39.30271958145975	40.0	40.0	40.0	40.0	40.0
4	39.19665990259409	40.0	40.0	40.0	40.0	40.0
5	39.202377950918454	40.0	40.0	40.0	40.0	40.0
6	39.31022778361169	40.0	40.0	40.0	40.0	40.0
7	39.32044977410935	40.0	40.0	40.0	40.0	40.0
8	39.31997327008232	40.0	40.0	40.0	40.0	40.0
9	39.345195346252794	40.0	40.0	40.0	40.0	40.0
10	39.358302470722336	40.0	40.0	40.0	40.0	40.0
11	39.3400680323729	40.0	40.0	40.0	40.0	40.0
12	39.34276387022445	40.0	40.0	40.0	40.0	40.0
13	39.241882092994985	40.0	40.0	40.0	40.0	40.0
14	39.36362887190119	40.0	40.0	40.0	40.0	40.0
15	39.319441282709676	40.0	40.0	40.0	40.0	40.0
16	39.32691847946259	40.0	40.0	40.0	40.0	40.0
17	39.36342978460223	40.0	40.0	40.0	40.0	40.0
18	39.318488274655614	40.0	40.0	40.0	40.0	40.0
19	39.36822419840844	40.0	40.0	40.0	40.0	40.0
20	39.03379996458857	40.0	40.0	40.0	40.0	40.0
21	39.237835072491436	40.0	40.0	40.0	40.0	40.0
22	39.22190156112184	40.0	40.0	40.0	40.0	40.0
23	39.332763813109246	40.0	40.0	40.0	40.0	40.0
24	39.34767251444811	40.0	40.0	40.0	40.0	40.0
25	39.13196632160921	40.0	40.0	40.0	40.0	40.0
26	39.30267225742967	40.0	40.0	40.0	40.0	40.0
27	39.31295952245158	40.0	40.0	40.0	40.0	40.0
28	39.0735129851427	40.0	40.0	40.0	40.0	40.0
29	39.267838507563276	40.0	40.0	40.0	40.0	40.0
30	38.934236732748964	40.0	40.0	40.0	40.0	40.0
31	39.09385252689923	40.0	40.0	40.0	40.0	40.0
32	39.15429347262917	40.0	40.0	40.0	40.0	40.0
33	39.158311119596796	40.0	40.0	40.0	40.0	40.0
34	38.98080031462321	40.0	40.0	40.0	40.0	40.0
35	39.09170825877759	40.0	40.0	40.0	40.0	40.0
36	39.216173721618844	40.0	40.0	40.0	40.0	40.0
37	39.006449938927524	40.0	40.0	40.0	40.0	40.0
38	39.0320114426241	40.0	40.0	40.0	40.0	40.0
39	38.999246895176455	40.0	40.0	40.0	40.0	40.0
40	38.662792623652386	40.0	40.0	40.0	40.0	40.0
41	38.845900719080475	40.0	40.0	40.0	40.0	40.0
42	38.60667285142784	40.0	40.0	40.0	40.0	40.0
43	38.95315655360303	40.0	40.0	40.0	40.0	40.0
44	38.48785037120806	40.0	40.0	40.0	40.0	40.0
45	38.81217989985256	40.0	40.0	40.0	40.0	40.0
46	38.468925654764675	40.0	40.0	40.0	40.0	40.0
47	37.9357421264645	40.0	40.0	40.0	24.0	40.0
48	38.327960424055945	40.0	40.0	40.0	40.0	40.0
49	37.98796500959128	40.0	40.0	40.0	40.0	40.0
50	38.323296559298235	40.0	40.0	40.0	40.0	40.0
51	37.94972719328521	40.0	40.0	40.0	40.0	40.0
52	38.014707982176795	40.0	40.0	40.0	40.0	40.0
53	37.28062252313778	40.0	40.0	40.0	24.0	40.0
54	36.65742216216966	40.0	40.0	40.0	24.0	40.0
55	36.64040835742371	40.0	40.0	40.0	24.0	40.0
56	36.49485269579706	40.0	40.0	40.0	24.0	40.0
57	36.58134144042925	40.0	40.0	40.0	24.0	40.0
58	36.584934802989245	40.0	40.0	40.0	24.0	40.0
59	36.182997128736865	40.0	40.0	40.0	24.0	40.0
60	35.56982619841987	40.0	40.0	40.0	24.0	40.0
61	35.49543771872065	40.0	40.0	40.0	24.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.07502792047583284
1101	2	-0.06158927291916427
1101	3	0.06983144557732857
1101	4	0.029923504100061393
1101	5	-0.012929327104394872
1101	6	0.06295409546806496
1101	7	4.220586884429167E-4
1101	8	0.1256636348084541
1101	9	-0.04403751813791956
1101	10	0.10134147207626398
1101	11	0.09812714343828333
1101	12	0.01676869960405014
1101	13	0.06878155484380954
1101	14	0.08193421583533933
1101	15	0.04225169727529021
1101	16	0.09949939921761342
1101	17	0.09873540660375824
1101	18	0.06483025773075468
1101	19	0.08451105250726698
1101	20	0.09869282850811345
1101	21	0.08392914538990226
1101	22	0.07833211272470919
1101	23	0.04830158371224513
1101	24	0.13798783961021854
1101	25	0.015604720123846505
1101	26	0.07205436788022723
1101	27	0.08068971361976196
1101	28	0.13051098312958942
1101	29	0.08450737008992348
1101	30	0.06475956809762096
1101	31	0.09803129013424439
1101	32	0.10219131111947632
1101	33	0.11049148152363841
1101	34	0.05816201795474285
1101	35	0.07341628342127393
1101	36	0.09640553074372349
1101	37	0.1086481178963723
1101	38	0.07218105445804923
1101	39	0.051280926834877505
1101	40	0.14057573441362337
1101	41	0.13567602340846463
1101	42	0.23131656771766274
1101	43	0.10249607640238168
1101	44	0.1807426635000553
1101	45	0.24937718314011192
1101	46	0.07758853969237123
1101	47	0.05425729080381814
1101	48	0.39864347501161035
1101	49	0.350134524495779
1101	50	0.27982085635245113
1101	51	0.33414254564178947
1101	52	0.234767360979788
1101	53	0.16480396796146124
1101	54	0.25622890913342644
1101	55	0.18404509459087848
1101	56	0.4566299229082418
1101	57	0.4305290114240421
1101	58	0.6242646259487543
1101	59	0.27389261717070923
1101	60	0.49330463566446525
1101	61	0.5035034054411014
1102	1	-0.09573391366921413
1102	2	0.007409274091948248
1102	3	-0.009427663974463485
1102	4	-0.02369746025357955
1102	5	0.09559842345331049
1102	6	-0.046943634260699696
1102	7	-0.005372555370549037
1102	8	-0.08784297759798676
1102	9	-0.053011748783809765
1102	10	0.07708937244674985
1102	11	0.07751191517111522
1102	12	-0.051555823775430554
1102	13	-0.09257939654195013
1102	14	0.05009826650569238
1102	15	-0.013757549829094273
1102	16	-0.08360270337654185
1102	17	0.03495614360512178
1102	18	-0.01939438876068067
1102	19	-0.025245649953888005
1102	20	-0.025365007972936837
1102	21	-0.13107317212419645
1102	22	-0.0018894505485249624
1102	23	-0.0023129241172483717
1102	24	0.03167087636337129
1102	25	-0.027796995282876935
1102	26	-0.051905540082266555
1102	27	-0.07552565516463261
1102	28	-0.0038961636120831145
1102	29	-0.029651525306988447
1102	30	-0.05401650393594082
1102	31	-0.044416961083008744
1102	32	-0.04874229321742973
1102	33	0.015687494761841947
1102	34	-0.09757492921294642
1102	35	-0.20869818201078516
1102	36	-0.12087694011520966
1102	37	-0.12628826363211942
1102	38	-0.005767241069307261
1102	39	-0.044364378111083624
1102	40	0.012956258114428465
1102	41	0.04102326480162333
1102	42	0.0850465540548484
1102	43	-0.06530903054435555
1102	44	-0.09366962193862349
1102	45	-0.02912438301487441
1102	46	-0.08973406528272676
1102	47	-0.14753462257024808
1102	48	0.15019040770042125
1102	49	0.04911883489348412
1102	50	-0.22805046710429622
1102	51	0.03149904944066151
1102	52	0.058850508412575664
1102	53	0.23645678586095897
1102	54	0.0118108028134003
1102	55	-0.10047040470112023
1102	56	-0.17092458648964737
1102	57	0.070407850384548
1102	58	-0.16826016380139208
1102	59	0.1520282264920283
1102	60	0.10712964326731367
1102	61	0.2710848579326992
1103	1	-0.02765959072465307
1103	2	-0.05680755877617827
1103	3	-0.009017423381230572
1103	4	-0.051890228897292445
1103	5	-0.1358073301742806
1103	6	-0.05371188524587467
1103	7	-0.09579590758771417
1103	8	0.011100152979473421
1103	9	0.010689898223049
1103	10	0.0766115643521772
1103	11	-0.08068151019734415
1103	12	-0.11552564972377155
1103	13	-0.05631067784460697
1103	14	-0.04655096700503947
1103	15	0.07989205255837817
1103	16	-0.12735037339228228
1103	17	-0.06153678288124098
1103	18	-0.05750638026050581
1103	19	-0.043298327120091074
1103	20	-0.11215958022458494
1103	21	-0.05922630857297406
1103	22	-0.08722367606910808
1103	23	-0.09139512024401597
1103	24	0.009900810400111482
1103	25	-0.004291607689481225
1103	26	0.04622547356421336
1103	27	-0.016611498890938492
1103	28	0.03234301880807777
1103	29	0.02403575543760894
1103	30	-0.010768859568401012
1103	31	-0.07504503498773829
1103	32	-0.042866403739303394
1103	33	-0.03465499862925725
1103	34	-0.06754202436255952
1103	35	-0.04283760957551408
1103	36	0.004201613639665425
1103	37	-0.07149820807843099
1103	38	-0.07615323512349903
1103	39	-0.03408470812017583
1103	40	-0.08834402421186383
1103	41	0.0236229995632371
1103	42	-0.06009628031034708
1103	43	-0.05689955580326256
1103	44	0.19077701231437771
1103	45	0.016126761924276423
1103	46	0.043252369267136714
1103	47	-0.1159914468388763
1103	48	0.26470464539491445
1103	49	0.16172889755024755
1103	50	-0.08789272900808243
1103	51	-0.05189100944565439
1103	52	-0.1807161447384189
1103	53	-0.18061714272202778
1103	54	-0.12127208205752282
1103	55	0.10610710205154561
1103	56	0.26752733358074465
1103	57	0.1746416339391743
1103	58	0.22231266680969242
1103	59	0.11820448422610497
1103	60	0.07786912513436306
1103	61	0.1287863931983324
1104	1	0.011626019099459484
1104	2	-0.017502268482814998
1104	3	-0.03446046976585393
1104	4	-0.09112195495384867
1104	5	-0.030388693000631406
1104	6	-0.04402312236627637
1104	7	0.024061407652894218
1104	8	-0.024436802496722976
1104	9	-0.08426117239787345
1104	10	0.03537080170277562
1104	11	-0.04029637291487376
1104	12	0.018109894180952324
1104	13	-0.06608874933642284
1104	14	0.007751112317976094
1104	15	-0.12168047122631975
1104	16	-0.09952789041062715
1104	17	-0.07972425102348524
1104	18	0.04018448313593126
1104	19	-0.044214758350662464
1104	20	-0.12896832969974525
1104	21	0.043954184200188706
1104	22	0.07785935752728079
1104	23	-0.04575869085390849
1104	24	-0.043012777884875675
1104	25	0.0881118601177846
1104	26	-0.03952300963148048
1104	27	0.02088101659267494
1104	28	-0.06425171031435184
1104	29	-0.07709253943770733
1104	30	0.023988206415658908
1104	31	0.04298457543194445
1104	32	-0.005090218043626749
1104	33	0.10438724522655463
1104	34	-0.051787333675406444
1104	35	-0.17749436624368542
1104	36	0.07127455305551678
1104	37	0.06647491125083604
1104	38	0.020322325364389826
1104	39	0.011476528304626754
1104	40	-0.018884726102903926
1104	41	0.08430779438518954
1104	42	0.06501585594962478
1104	43	0.06274385309560415
1104	44	0.09366380462251556
1104	45	0.04218804649836727
1104	46	-0.10701004943131665
1104	47	-0.1363464811470223
1104	48	-0.3059325966628421
1104	49	0.037020414501142795
1104	50	0.0696805089740522
1104	51	-0.014150112867270082
1104	52	0.008633586090446954
1104	53	-0.15674134032723686
1104	54	-0.10943068302317727
1104	55	-0.08721998375074946
1104	56	0.1546768083056307
1104	57	-0.0073956488775124285
1104	58	-0.045772242335424096
1104	59	0.20065343495510035
1104	60	0.12302263652707524
1104	61	-0.12000269806686248
1201	1	0.014252201426906197
1201	2	0.07326983071776283
1201	3	0.08413250735807765
1201	4	0.11415632299846123
1201	5	-0.09318400881295474
1201	6	0.04494701827854186
1201	7	0.04067332117165279
1201	8	-0.003856216930692824
1201	9	0.057660157598121486
1201	10	0.011199867589596124
1201	11	0.03606112204081313
1201	12	-0.015387698596107668
1201	13	0.0697046445286702
1201	14	0.0289271184137192
1201	15	-0.022121795433065472
1201	16	-0.0401329632445524
1201	17	0.007626707608892502
1201	18	0.08118841855169023
1201	19	-0.01877636069701083
1201	20	-0.0020099335785488393
1201	21	-0.08744485716743355
1201	22	-0.008510141617101397
1201	23	-0.03914813951258367
1201	24	0.07515957374420168
1201	25	-0.0315477125841781
1201	26	-0.028554820731528707
1201	27	-0.029904523468111677
1201	28	-0.0021585011945788324
1201	29	-0.014213151736470309
1201	30	-0.08698828325307062
1201	31	0.0682162520169598
1201	32	-0.03122220878081805
1201	33	0.04973599457738942
1201	34	0.07498855195277088
1201	35	0.050516578000689094
1201	36	-0.006506576955807475
1201	37	-0.032404538174070296
1201	38	0.04080713282912285
1201	39	-0.05987576270755568
1201	40	0.013068976891332795
1201	41	-0.049433553659660845
1201	42	-0.0542241554586127
1201	43	0.01251329392176359
1201	44	-0.011780967595875325
1201	45	-0.04419821381374334
1201	46	0.03367122020319613
1201	47	0.6359059643871632
1201	48	0.13272232760860447
1201	49	-0.13419571197703561
1201	50	-0.056084651635181615
1201	51	-0.19475648402656986
1201	52	-0.01804034906990637
1201	53	-0.08466645675083839
1201	54	0.04158956659583879
1201	55	0.0031655443909812675
1201	56	0.35593373625495417
1201	57	0.3040100863151949
1201	58	0.3716627775438539
1201	59	0.22969761207222206
1201	60	0.24067193330297698
1201	61	0.22842925478477127
1202	1	-0.3318664633595674
1202	2	-0.2131310504130255
1202	3	-0.2258288180094823
1202	4	-0.11900592271687316
1202	5	-0.22305275703984506
1202	6	-0.0933695504847023
1202	7	-0.17595833528746851
1202	8	-0.16875742347910005
1202	9	-0.13174854189956164
1202	10	-0.22161401439228
1202	11	-0.06548377724660526
1202	12	-0.1894678723190495
1202	13	-0.0994149965106601
1202	14	-0.09211676290063764
1202	15	0.003910571103993732
1202	16	-0.12581740411033593
1202	17	-0.055289972964509104
1202	18	-0.1632966915062184
1202	19	-0.12698136962513473
1202	20	-0.17340332593206398
1202	21	-0.130266478247826
1202	22	-0.09030052260603583
1202	23	-0.17242699320816257
1202	24	-0.08705537293737109
1202	25	-0.04147555748513554
1202	26	-0.12111214429676664
1202	27	-0.017668508081698064
1202	28	-0.2078792803611762
1202	29	-0.11160063233657525
1202	30	-0.15999551753706953
1202	31	-0.0797684533549301
1202	32	-0.11954493127468879
1202	33	-0.005179793183572201
1202	34	-0.0998519725536795
1202	35	-0.13431958674406275
1202	36	-0.08216878095093705
1202	37	-0.11086721110432762
1202	38	-0.0028176022475605578
1202	39	-0.01229855946338887
1202	40	-0.18800990767719838
1202	41	-0.05041606454863512
1202	42	-0.15362150705983169
1202	43	-0.0034733681278638073
1202	44	0.0018604972840563505
1202	45	-0.1584641259009203
1202	46	-0.09910386198561838
1202	47	-0.2903160204934281
1202	48	0.1341580242526348
1202	49	-0.10894872834804659
1202	50	-0.020701743569830455
1202	51	-0.09329554665694673
1202	52	-0.022761512062409395
1202	53	0.007020478983349676
1202	54	-0.12640415034090324
1202	55	-0.06863649739597832
1202	56	0.3090829861256239
1202	57	0.05386563009637513
1202	58	0.157763273179917
1202	59	-0.14370658998709018
1202	60	0.1793771258000163
1202	61	-0.3647397913243964
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2403	4	0.1893915380522273
2403	5	0.12633884064941014
2403	6	0.10959755591295561
2403	7	0.16614375127917924
2403	8	0.1786370626391971
2403	9	0.11401096404973998
2403	10	0.1454740611379819
2403	11	0.09204230483651088
2403	12	0.08602359172647311
2403	13	0.13438878431362156
2403	14	0.13094324744597685
2403	15	0.12226664542715326
2403	16	0.18449741309952827
2403	17	0.13071138502824198
2403	18	0.18827847231513317
2403	19	0.08350858553954765
2403	20	0.04076560405586349
2403	21	0.09813982025191592
2403	22	0.07257184763021485
2403	23	0.07194057016483413
2403	24	0.10063000385172671
2403	25	0.1107909970932397
2403	26	0.07023550184911898
2403	27	0.1650887561017811
2403	28	0.1604423052570354
2403	29	0.09412018159686397
2403	30	0.1587442436286466
2403	31	-0.003020307122824306
2403	32	0.12600225358477246
2403	33	0.14224271500749808
2403	34	0.005096078834490925
2403	35	0.2076402339146668
2403	36	0.06405793917322455
2403	37	0.0473669672022794
2403	38	0.10609611132374397
2403	39	0.1266833770773914
2403	40	0.16921817043971998
2403	41	0.10490112375968863
2403	42	0.26919654346611566
2403	43	0.12689501435187367
2403	44	0.10831311842563451
2403	45	0.1792896894120659
2403	46	0.1158620804182462
2403	47	0.028177469763512875
2403	48	-0.23364191970322423
2403	49	0.13922498694769558
2403	50	0.21374061718202597
2403	51	0.09018975253257366
2403	52	0.09398922082257144
2403	53	0.2813953712061519
2403	54	0.31047397519798636
2403	55	0.24960371643399526
2403	56	0.1590587362549556
2403	57	-0.2262990534697522
2403	58	-0.13724179234861822
2403	59	-0.03599459222885315
2403	60	-0.0690390882024019
2403	61	0.23686339456971695
2404	1	0.039118852829503226
2404	2	-0.032730644164367106
2404	3	-0.09046935485826424
2404	4	-0.07606258918226416
2404	5	0.05171016031292197
2404	6	0.031462697868789746
2404	7	0.064513887977391
2404	8	0.03195922352247749
2404	9	0.017471137551311244
2404	10	-0.030597179661178586
2404	11	0.017180317454808858
2404	12	-0.025622044445974268
2404	13	0.06648329009700404
2404	14	0.05552263756164422
2404	15	0.11070315962273725
2404	16	0.069601908367666
2404	17	0.0418871210955416
2404	18	0.004856416584324563
2404	19	0.0709625813334469
2404	20	0.08670080384555945
2404	21	0.04779478870617737
2404	22	0.04276440809288573
2404	23	-0.03877183782675786
2404	24	0.06594283245319588
2404	25	-0.0024516422337867994
2404	26	0.019105112784977507
2404	27	0.043470065250041046
2404	28	-0.1465470480552682
2404	29	-0.04444448717285354
2404	30	0.1175014728610364
2404	31	0.03161428804016708
2404	32	0.03717141762262344
2404	33	2.0711852926069696E-5
2404	34	-0.039166539462044625
2404	35	-0.030240943793003794
2404	36	-0.03317523643140419
2404	37	-0.02981494657269934
2404	38	0.17450765180744554
2404	39	0.01432138969568797
2404	40	0.0727769243829357
2404	41	0.028629436062530544
2404	42	-0.07588429249602768
2404	43	0.0014415443203290579
2404	44	0.0067161152710539795
2404	45	0.07510002064234556
2404	46	0.058251670323606675
2404	47	-0.11718924947939513
2404	48	-0.340138370807324
2404	49	-0.04669443997133982
2404	50	0.08542174231346422
2404	51	0.049673191018378304
2404	52	0.011089641432455721
2404	53	0.007052574150421265
2404	54	0.04616666710124662
2404	55	-0.13554349702552315
2404	56	-0.08012041726765062
2404	57	-0.30087844358542526
2404	58	-0.17090701316880796
2404	59	-0.3014750075811179
2404	60	-0.17133601249262398
2404	61	-0.2175497232010457
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	8.0
14	22.0
15	83.0
16	279.0
17	585.0
18	838.0
19	1037.0
20	1235.0
21	1161.0
22	1354.0
23	1561.0
24	1842.0
25	2194.0
26	2572.0
27	3233.0
28	4158.0
29	4983.0
30	6875.0
31	9942.0
32	12213.0
33	12098.0
34	15970.0
35	25753.0
36	44358.0
37	65023.0
38	149825.0
39	485402.0
40	370989.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.341295867320838	27.916982505326082	20.92400860658647	21.817713020766615
2	28.603378119816288	19.970414321883364	20.313676726286786	31.11253083201356
3	31.11176023263935	18.597114201509807	17.8027200005222	32.48840556532864
4	30.64924489614415	18.946991374787554	19.430838785795938	30.972924943272357
5	28.217279308873337	20.202546848749954	20.544911728444923	31.03526211393179
6	27.27618385548873	20.358471368553836	22.344203989415735	30.0211407865417
7	28.48474167199062	22.27044377701243	22.396994760903496	26.847819790093446
8	26.640491582442134	23.791013819432717	23.57258894265878	25.995905655466373
9	26.92476213555397	23.460235167792245	23.19195687312183	26.423045823531954
10	28.68562402037218	22.640468736358642	22.530970721928078	26.1429365213411
11	27.33648119726532	23.453462935901236	22.601711987584785	26.608343879248658
12	28.186600282475503	22.000696805546376	22.374230270571065	27.438472641407056
13	28.496164713734494	22.583679900260528	21.771093666494505	27.149061719510474
14	28.776110829614726	22.504534539606542	21.910291589459145	26.809063041319593
15	28.076857488578998	22.106849500609094	22.459331931562925	27.356961079248983
16	28.702513803522052	22.491153262135146	21.34966501930086	27.456667915041944
17	29.046592139478605	22.758697218407743	21.471565193339064	26.723145448774595
18	28.16848660199593	21.99547484360632	22.16869711233664	27.66734144206111
19	29.586494048187284	22.443992418364008	21.16918095974765	26.80033257370106
20	28.246734437941466	22.459821490494804	22.34134822897977	26.952095842583958
21	28.44981980151649	22.711046815704723	21.502570592358147	27.336562790420636
22	29.61431731414915	22.485523334418524	21.194148465273546	26.706010886158783
23	29.224628404372417	22.411599935704594	21.56352067937725	26.80025098054574
24	29.107542226497703	21.842324491083094	21.474747326396283	27.57538595602292
25	28.848973517309577	23.271836572173633	21.203858050755837	26.67533185976095
26	28.278882141134943	23.370645883258145	21.499143679834987	26.851328295771925
27	28.40934959648105	22.894794601470473	21.661350872598	27.034504929450478
28	28.80564755183817	23.682331736555284	21.047525565175388	26.464495146431155
29	28.494777630094166	23.629132999290956	21.504936793862235	26.371152576752642
30	28.434561881472888	23.40948422518732	21.217728887159115	26.938225006180684
31	29.1105611732443	23.245889948783976	21.689418918025805	25.95412995994592
32	29.359093924328878	23.342006685743147	21.30201461659784	25.996884773330137
33	28.999994288432525	23.083463135503674	21.64161872349731	26.274923852566495
34	29.320010802933766	23.75111476648447	20.686802225534905	26.242072205046863
35	29.089020580241566	23.80415031743817	21.02884073260862	26.07798836971164
36	29.039656721276963	23.435594034887604	20.98926805228163	26.5354811915538
37	29.661396564764974	23.957219076806087	20.579262446831862	25.80212191159708
38	29.444521957941994	24.24328467933482	20.578201735812787	25.733991626910402
39	29.707333511206414	23.944408951421885	20.370302376074275	25.977955161297427
40	30.201951218716168	24.64823150915516	20.016106488858863	25.133710783269812
41	29.967860456122057	24.676789113514847	20.855863243344242	24.499487187018858
42	30.084130702443634	24.443351096163248	20.758033050123494	24.71448515126963
43	30.72528971689623	24.82381997938957	20.420890132368577	24.030000171345627
44	30.437510658105914	25.13509786691014	20.59729453415612	23.83009694082783
45	31.11408110196452	24.861108051367786	20.074037629131368	23.950773217536327
46	31.48592680108878	24.998286542340356	19.038554429206457	24.47723222736441
47	31.651861588635054	25.075779642997308	19.192831551746785	24.079527216620853
48	31.601518611806696	25.832637751684285	19.179368681120078	23.38647495538894
49	32.41940840066809	25.261893630267146	18.705149262438674	23.613548706626098
50	31.904718776951242	25.864295895945883	19.148934434188185	23.082050892914697
51	32.120043113823264	25.856870918812362	18.967716036237153	23.055369931127217
52	32.49333179938201	26.238971665144955	18.76389633426431	22.50380020120872
53	32.835560284336054	25.33469539618405	18.374059230151634	23.45568508932826
54	32.4786450314256	24.974522537253392	18.213795281141458	24.333037150179546
55	33.53274700491925	25.27666199137887	18.775890528095378	22.4147004756065
56	32.472035985845224	26.175900156087707	19.87103385871166	21.481029999355414
57	33.22261142157307	26.764268399052536	18.80795663813354	21.20516354124085
58	32.66410627345293	27.350841592600478	19.076398119114586	20.908654014832003
59	32.34532181564353	27.98767616982147	18.35127974784451	21.315722266690493
60	32.26718190066515	26.095062630957123	20.635007408664546	21.002748059713184
61	32.635711855403876	27.66236425958699	18.34671053114696	21.355213353862172
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	31.5
2	36.0
3	41.0
4	38.0
5	35.0
6	35.0
7	32.0
8	29.0
9	38.5
10	48.0
11	41.5
12	42.5
13	50.0
14	55.0
15	60.0
16	70.0
17	90.5
18	101.0
19	129.0
20	213.5
21	270.0
22	355.0
23	440.0
24	560.5
25	944.0
26	1207.0
27	1573.5
28	1940.0
29	2559.0
30	4016.5
31	4855.0
32	5650.0
33	6445.0
34	7409.5
35	9419.5
36	10465.0
37	12466.0
38	14467.0
39	16646.5
40	21478.5
41	24131.0
42	26929.5
43	33321.5
44	36915.0
45	40056.0
46	43197.0
47	46924.5
48	54335.5
49	58019.0
50	62071.0
51	66123.0
52	71004.5
53	79569.5
54	83253.0
55	86060.5
56	88868.0
57	89863.5
58	90495.5
59	90132.0
60	88386.5
61	82651.5
62	78662.0
63	73387.0
64	68112.0
65	61657.0
66	48632.5
67	42063.0
68	36209.0
69	30355.0
70	25606.5
71	17213.0
72	13568.0
73	11004.0
74	8440.0
75	6750.5
76	4009.0
77	2957.0
78	2276.0
79	1595.0
80	1246.0
81	673.0
82	449.0
83	350.5
84	201.0
85	150.0
86	124.0
87	98.0
88	78.5
89	47.5
90	36.0
91	36.0
92	36.0
93	40.0
94	47.0
95	50.0
96	48.5
97	47.0
98	54.0
99	158.0
100	255.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0016318631062677415
2	0.0
3	4.0796577656693536E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	8.159315531338707E-4
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	8.159315531338707E-5
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	8.159315531338707E-5
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	8.159315531338707E-5
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1225593.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.44792225899576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.434990278921	67.87632465877775
2	10.898905478284304	17.31790790293749
3	2.1339002440246415	5.0860182208716544
4	0.5624752227676862	1.7874995108423382
5	0.2277979044974737	0.9049035103638714
6	0.13961505280713876	0.665527551696429
7	0.0968662194531727	0.5387073910846941
8	0.07325311451552434	0.4655846197806953
9	0.0646564129523535	0.4623135902805696
>10	0.36444558579832215	4.457750259632336
>50	0.001769664509731392	0.08819388825871302
>100	0.001104017890567069	0.16954715593929098
>500	1.1040178905670688E-4	0.07481827419359297
>1k	1.1040178905670688E-4	0.10490346534060634
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.7132873637496298	0.0	0.0	0.0	0.0
2	0.7141032953027636	0.0	0.0	0.0	0.0
3	0.7144296679240172	0.0	0.0	0.0	0.0
4	0.7159799378749715	0.0	0.0	0.0	0.0
5	0.7167958694281055	0.0	0.0	0.0	0.0
6	0.7198964093300141	0.0	0.0	0.0	0.0
7	0.7230785423872362	0.0	0.0	0.0	0.0
8	0.7331960936460962	0.0	0.0	0.0	8.159315531338707E-5
9	0.7351543293736175	0.0	0.0	0.0	8.159315531338707E-5
10	0.7390708008286601	0.0	0.0	0.0	8.159315531338707E-5
11	0.7446191353899704	0.0	8.159315531338707E-5	0.0	8.159315531338707E-5
12	0.7583267854826194	0.0	8.159315531338707E-5	8.159315531338707E-5	8.159315531338707E-5
13	0.7768484317387583	0.0	8.159315531338707E-5	8.159315531338707E-5	8.159315531338707E-5
14	0.8033662072156091	0.0	8.159315531338707E-5	8.159315531338707E-5	8.159315531338707E-5
15	0.8368194008940978	0.0	8.159315531338707E-5	8.159315531338707E-5	8.159315531338707E-5
16	0.876065708599837	0.0	8.159315531338707E-5	8.159315531338707E-5	8.159315531338707E-5
17	0.923716111302855	0.0	1.6318631062677415E-4	8.159315531338707E-5	8.159315531338707E-5
18	0.9932334796298609	8.159315531338707E-5	1.6318631062677415E-4	8.159315531338707E-5	1.6318631062677415E-4
19	1.0753977870304416	8.159315531338707E-5	1.6318631062677415E-4	8.159315531338707E-5	1.6318631062677415E-4
20	1.1785315353465629	8.159315531338707E-5	1.6318631062677415E-4	8.159315531338707E-5	1.6318631062677415E-4
21	1.3209115913684233	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	1.6318631062677415E-4
22	1.4849138335483314	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	1.6318631062677415E-4
23	1.6847354709108162	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	2.447794659401612E-4
24	1.9504027846112046	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	2.447794659401612E-4
25	2.248707360436948	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	2.447794659401612E-4
26	2.6100018521646255	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	2.447794659401612E-4
27	3.0332255487751643	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	2.447794659401612E-4
28	3.540408602203178	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	2.447794659401612E-4
29	4.111887062018141	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	2.447794659401612E-4
30	4.760063087827688	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	2.447794659401612E-4
31	5.493014402007844	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	2.447794659401612E-4
32	6.327386008242541	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	2.447794659401612E-4
33	7.274845727741591	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	2.447794659401612E-4
34	8.32005404730608	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	2.447794659401612E-4
35	9.408017180254783	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	2.447794659401612E-4
36	10.619675536658582	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	2.447794659401612E-4
37	11.886817238675482	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	2.447794659401612E-4
38	13.25170754075782	8.159315531338707E-5	2.447794659401612E-4	8.159315531338707E-5	3.263726212535483E-4
39	14.690602834709402	1.6318631062677415E-4	2.447794659401612E-4	8.159315531338707E-5	3.263726212535483E-4
40	16.207419591985268	1.6318631062677415E-4	2.447794659401612E-4	8.159315531338707E-5	3.263726212535483E-4
41	17.78486006365898	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	4.0796577656693536E-4
42	19.463149675300038	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	4.0796577656693536E-4
43	21.175300446396154	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	4.0796577656693536E-4
44	22.956315840576767	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	4.0796577656693536E-4
45	24.758708641449488	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	4.0796577656693536E-4
46	26.63518802734676	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	4.0796577656693536E-4
47	28.479764489516505	2.447794659401612E-4	3.263726212535483E-4	8.159315531338707E-5	4.0796577656693536E-4
48	30.37705013001869	2.447794659401612E-4	3.263726212535483E-4	8.159315531338707E-5	4.0796577656693536E-4
49	32.31545872079883	2.447794659401612E-4	3.263726212535483E-4	8.159315531338707E-5	4.895589318803224E-4
50	34.25917086667434	2.447794659401612E-4	3.263726212535483E-4	8.159315531338707E-5	4.895589318803224E-4
>>END_MODULE