##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-33A_S38_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1225593
Sequences flagged as poor quality	0
Sequence length	61
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.509217170789974	40.0	40.0	40.0	40.0	40.0
2	39.23777958914582	40.0	40.0	40.0	40.0	40.0
3	39.22932164266604	40.0	40.0	40.0	40.0	40.0
4	39.113731883259774	40.0	40.0	40.0	40.0	40.0
5	39.1443293165023	40.0	40.0	40.0	40.0	40.0
6	39.290521404740396	40.0	40.0	40.0	40.0	40.0
7	39.308145526288094	40.0	40.0	40.0	40.0	40.0
8	39.30867424993452	40.0	40.0	40.0	40.0	40.0
9	39.318697153133215	40.0	40.0	40.0	40.0	40.0
10	39.316144919235015	40.0	40.0	40.0	40.0	40.0
11	39.319030053206895	40.0	40.0	40.0	40.0	40.0
12	39.328540551390226	40.0	40.0	40.0	40.0	40.0
13	39.1559155445568	40.0	40.0	40.0	40.0	40.0
14	39.31993736909398	40.0	40.0	40.0	40.0	40.0
15	39.234763906125444	40.0	40.0	40.0	40.0	40.0
16	39.410398068526824	40.0	40.0	40.0	40.0	40.0
17	39.36015953093727	40.0	40.0	40.0	40.0	40.0
18	39.44310386890265	40.0	40.0	40.0	40.0	40.0
19	39.39410228354764	40.0	40.0	40.0	40.0	40.0
20	39.3824801545048	40.0	40.0	40.0	40.0	40.0
21	39.38517272863014	40.0	40.0	40.0	40.0	40.0
22	39.437539215710274	40.0	40.0	40.0	40.0	40.0
23	39.432519604795395	40.0	40.0	40.0	40.0	40.0
24	39.43738255685207	40.0	40.0	40.0	40.0	40.0
25	39.39003241696061	40.0	40.0	40.0	40.0	40.0
26	39.36175875678141	40.0	40.0	40.0	40.0	40.0
27	39.38268250552998	40.0	40.0	40.0	40.0	40.0
28	39.390649261214776	40.0	40.0	40.0	40.0	40.0
29	39.39001936205575	40.0	40.0	40.0	40.0	40.0
30	39.38532938748834	40.0	40.0	40.0	40.0	40.0
31	39.34212091616058	40.0	40.0	40.0	40.0	40.0
32	39.36800552875221	40.0	40.0	40.0	40.0	40.0
33	39.36695787263798	40.0	40.0	40.0	40.0	40.0
34	39.362107975486154	40.0	40.0	40.0	40.0	40.0
35	39.361389955719396	40.0	40.0	40.0	40.0	40.0
36	39.32721874227415	40.0	40.0	40.0	40.0	40.0
37	39.3438702734105	40.0	40.0	40.0	40.0	40.0
38	39.325769647835784	40.0	40.0	40.0	40.0	40.0
39	38.04767814437582	40.0	40.0	40.0	40.0	40.0
40	39.29842288590095	40.0	40.0	40.0	40.0	40.0
41	39.32302811781725	40.0	40.0	40.0	40.0	40.0
42	39.28827596110617	40.0	40.0	40.0	40.0	40.0
43	39.30369380373419	40.0	40.0	40.0	40.0	40.0
44	39.256056455936026	40.0	40.0	40.0	40.0	40.0
45	39.102181556193614	40.0	40.0	40.0	40.0	40.0
46	39.12239707635406	40.0	40.0	40.0	40.0	40.0
47	39.20965932409862	40.0	40.0	40.0	40.0	40.0
48	39.26603366696774	40.0	40.0	40.0	40.0	40.0
49	39.247280296150514	40.0	40.0	40.0	40.0	40.0
50	39.22693749066778	40.0	40.0	40.0	40.0	40.0
51	39.18618334145185	40.0	40.0	40.0	40.0	40.0
52	39.186388956203245	40.0	40.0	40.0	40.0	40.0
53	39.10583040209923	40.0	40.0	40.0	40.0	40.0
54	39.03778171056786	40.0	40.0	40.0	40.0	40.0
55	39.07410371958717	40.0	40.0	40.0	40.0	40.0
56	39.065774690292784	40.0	40.0	40.0	40.0	40.0
57	38.98948835380098	40.0	40.0	40.0	40.0	40.0
58	38.93084572121414	40.0	40.0	40.0	40.0	40.0
59	38.87565937468638	40.0	40.0	40.0	40.0	40.0
60	38.84032953843568	40.0	40.0	40.0	40.0	40.0
61	38.79070784510029	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.007239051007438491
1101	2	-0.04062861385622085
1101	3	0.05001125961628361
1101	4	0.028290972081180144
1101	5	0.10211419810489986
1101	6	-0.04254199744541154
1101	7	0.035045319625211846
1101	8	0.02306149599867524
1101	9	0.02590139472495423
1101	10	0.07977993450703735
1101	11	0.09069468461026275
1101	12	0.01651897461167806
1101	13	0.05709839703550301
1101	14	0.03567472453273268
1101	15	-0.0063739996532490295
1101	16	0.020246354629648522
1101	17	0.010658088248867159
1101	18	0.032227136395995615
1101	19	0.030763838115916542
1101	20	0.0189077413591221
1101	21	0.0664705509354846
1101	22	0.06426891364188236
1101	23	0.011736823212110892
1101	24	0.019414727435957957
1101	25	0.17639330606501602
1101	26	0.07543507755776346
1101	27	-0.04780054400709588
1101	28	0.008752240497557295
1101	29	0.06799400425551028
1101	30	0.05865088610665481
1101	31	0.024258226996252574
1101	32	-0.013721774270734954
1101	33	0.012631681656209537
1101	34	0.10727647415897223
1101	35	0.018045047380248036
1101	36	0.00924400715913265
1101	37	-0.05248557266114062
1101	38	-0.04227636154670478
1101	39	1.1889279764439848
1101	40	-0.03255353379081072
1101	41	0.04217623682799854
1101	42	-0.032748342107439044
1101	43	-0.05579516437455112
1101	44	-0.022657145888636876
1101	45	-0.033189594438198355
1101	46	0.06457578144572551
1101	47	0.06467806723409097
1101	48	-0.07068763531214728
1101	49	0.045052877097312205
1101	50	-0.06621353695409482
1101	51	0.0489487410040681
1101	52	-0.03764674029908832
1101	53	0.08012091162135704
1101	54	-0.0218517274279435
1101	55	-0.02820206324485497
1101	56	0.07578223361501557
1101	57	-0.07184870183569814
1101	58	-0.03668878076108939
1101	59	0.02295016210702272
1101	60	0.04317120134054164
1101	61	0.18185521915054892
1102	1	0.06653682657171345
1102	2	0.05776646778594596
1102	3	0.11599926974831476
1102	4	0.0029784902116603007
1102	5	0.012080656312186022
1102	6	0.08385184223872244
1102	7	0.039845092145426975
1102	8	-0.0710661719318395
1102	9	0.029411520061977114
1102	10	0.015103248443807615
1102	11	-0.05721945707113463
1102	12	-0.06627091707402855
1102	13	-0.10548513069911536
1102	14	0.02414517160575258
1102	15	-0.039625577124844824
1102	16	-0.10217678965361898
1102	17	0.07983668092169438
1102	18	-0.017463936106175026
1102	19	0.028237843385952033
1102	20	-0.010281842769828131
1102	21	-0.0017909819646035885
1102	22	0.09137598661839519
1102	23	0.01672964752541617
1102	24	0.08545068173853565
1102	25	-0.09971028649624003
1102	26	-0.012283372850937724
1102	27	-0.06164926867303677
1102	28	0.0049626108471514385
1102	29	0.0434687758672041
1102	30	-0.0034465055902046515
1102	31	-0.08049187995367646
1102	32	0.06498470220888919
1102	33	0.08684084192553598
1102	34	0.03425750242116976
1102	35	-0.07634478335515382
1102	36	-0.017611358922025033
1102	37	-0.03312734873763645
1102	38	-0.0282608523725969
1102	39	1.1449108925078946
1102	40	0.01990758967116335
1102	41	0.024872726638911047
1102	42	0.02072929977893523
1102	43	0.05038536200399335
1102	44	0.016160035720950816
1102	45	-0.010697657920871961
1102	46	0.015452133302382265
1102	47	0.025795599945467984
1102	48	-0.004970713761430545
1102	49	-0.07395245987944321
1102	50	-0.003806293303988184
1102	51	-0.0028602907340840034
1102	52	-0.019157886999963125
1102	53	0.0012320942803540902
1102	54	-0.04808560444731569
1102	55	-0.13227865660751803
1102	56	0.01692464374041691
1102	57	-0.16819429769822136
1102	58	-0.1119476123387031
1102	59	-0.09510649234620416
1102	60	-0.01905497655911148
1102	61	-0.013536912522503997
1103	1	0.08407876138737436
1103	2	0.047921347005768666
1103	3	0.036354306255347524
1103	4	-0.041375849709716306
1103	5	0.0443073485464609
1103	6	0.031129326087700804
1103	7	0.036336286969515186
1103	8	-0.019472046122324116
1103	9	0.010210064681523079
1103	10	0.05971949411506472
1103	11	0.02560366212309617
1103	12	0.0421695642235278
1103	13	-0.12897352839855358
1103	14	-0.030014689573746978
1103	15	0.01577010280254143
1103	16	-0.05395136774063758
1103	17	-0.02854185260867581
1103	18	0.0064186271329873534
1103	19	-0.03530566691564019
1103	20	0.07581274585093212
1103	21	0.019565576118125705
1103	22	-0.008804792387849147
1103	23	0.05444015476196995
1103	24	-0.004637054727297141
1103	25	-0.042360245998210644
1103	26	-0.05814275599363583
1103	27	0.009384432415266986
1103	28	-0.03927907300817424
1103	29	0.026281799143681894
1103	30	-0.051898546550226854
1103	31	-0.08919697884955724
1103	32	0.014764143979789424
1103	33	0.0031593410121644183
1103	34	-0.06242780194134667
1103	35	-0.062121366903120645
1103	36	-0.023682240106140284
1103	37	-0.007265332161381366
1103	38	-0.015041930727697661
1103	39	1.3075274407299489
1103	40	0.06735696550493486
1103	41	0.062481555894720486
1103	42	0.013677971022197255
1103	43	0.008844548521331319
1103	44	0.02397172757785171
1103	45	-0.06361699545876576
1103	46	0.03614843796777478
1103	47	0.008525503433070014
1103	48	-0.09607941216238913
1103	49	0.06844846698461993
1103	50	0.008691211305333013
1103	51	0.04590723948750508
1103	52	0.023486485440727733
1103	53	0.03160061488207333
1103	54	0.09914646410820893
1103	55	-0.06888753258554203
1103	56	0.01742937428232949
1103	57	-0.02577925615302945
1103	58	-0.03534723784223104
1103	59	0.06934731686970252
1103	60	0.07810421515321764
1103	61	0.14159990023220104
1104	1	0.035188675383039936
1104	2	-0.00834022841851123
1104	3	-0.0609312752017388
1104	4	-0.04677725629321827
1104	5	-0.06570674606270899
1104	6	-0.025982852790662037
1104	7	0.007622075139394724
1104	8	-0.026779879403775908
1104	9	0.03960997120608312
1104	10	0.1204702524408674
1104	11	0.026285827905809356
1104	12	0.03486770294654207
1104	13	0.06406418664195712
1104	14	0.03543847935844724
1104	15	-0.010081618705790163
1104	16	-0.04912788831646964
1104	17	-0.00696310029477587
1104	18	0.0052125536858866894
1104	19	0.031911957881099795
1104	20	-0.08117360696518716
1104	21	0.04047166356550491
1104	22	0.04266492809044564
1104	23	0.04406162536670877
1104	24	0.007609354791640044
1104	25	0.028917256015475346
1104	26	0.02247007481724239
1104	27	0.007822481793020586
1104	28	0.11447778266632724
1104	29	0.04843359243143652
1104	30	0.029745812304533104
1104	31	0.0252220119617661
1104	32	-0.015952860983965422
1104	33	0.06447716830736283
1104	34	0.0920831550241914
1104	35	-0.01399053983003995
1104	36	-0.05675791537867525
1104	37	-0.038335228298045365
1104	38	0.01622504155629656
1104	39	1.1699989642785482
1104	40	-0.019121459509186423
1104	41	-0.03487650398018616
1104	42	-0.02353901789179247
1104	43	-0.02081975492815502
1104	44	0.08828777256903919
1104	45	0.08413473827181406
1104	46	0.07525475193057218
1104	47	0.10085350682027894
1104	48	0.06023536160620324
1104	49	0.09251564497702702
1104	50	0.0256612049010414
1104	51	0.1360424314172093
1104	52	-0.037395928438044734
1104	53	0.11815559013523824
1104	54	-0.0941371889343472
1104	55	0.013137134371717707
1104	56	0.003881067790103998
1104	57	-0.08370254181959069
1104	58	0.06653950160009714
1104	59	-0.01570108452989416
1104	60	0.05910265422055261
1104	61	0.11212290055910046
1201	1	0.04591928138172818
1201	2	0.027817601122009705
1201	3	0.08206143927735354
1201	4	0.04154957477123844
1201	5	0.0654430061912663
1201	6	0.10019042895019936
1201	7	-0.015279000980783053
1201	8	-0.05157017857199975
1201	9	0.06721534840152543
1201	10	0.039768422440609186
1201	11	-0.08032424454500386
1201	12	0.006458055750499625
1201	13	0.07876293258735245
1201	14	-0.012656228156515681
1201	15	0.1030740888176922
1201	16	0.024657964619450468
1201	17	0.09956502866937456
1201	18	0.0650602984308577
1201	19	0.024068529535888672
1201	20	0.03329984760193838
1201	21	0.029651664672272204
1201	22	0.07108814385083662
1201	23	0.04046489161280675
1201	24	0.060257778922618854
1201	25	0.06451584786454134
1201	26	0.060743536195253967
1201	27	0.12856674149215053
1201	28	-0.046416657095633695
1201	29	0.033200351470760836
1201	30	0.09846766418476705
1201	31	0.06474165285773381
1201	32	0.011454042701572575
1201	33	0.011802671248815955
1201	34	0.03428107089270327
1201	35	0.0011370791689273574
1201	36	0.09798251532480862
1201	37	0.007835935682578565
1201	38	0.149002489431723
1201	39	1.2128362521832656
1201	40	0.07801642477384263
1201	41	0.023192960684077946
1201	42	0.1655232293604385
1201	43	0.04967984493222133
1201	44	0.07969807318457356
1201	45	-0.03136567038141891
1201	46	0.108281169659314
1201	47	0.016023906704496937
1201	48	0.021395745229426666
1201	49	0.07206728640083782
1201	50	0.02073847654476424
1201	51	-0.0866538924064173
1201	52	0.0945953408559177
1201	53	0.04912937673312001
1201	54	0.020970529503578916
1201	55	0.004689771887790073
1201	56	0.024524707272817636
1201	57	0.08252274743027499
1201	58	0.02585775307507987
1201	59	0.07739576202933307
1201	60	0.04718626211777632
1201	61	0.07231406300726917
1202	1	0.08162499955338376
1202	2	0.03747895819692815
1202	3	-0.0072829068001141195
1202	4	0.0421039320297254
1202	5	-0.05258711162660035
1202	6	0.04775334302325973
1202	7	-0.023390452343363677
1202	8	-0.03408367505915777
1202	9	-0.0321538420595644
1202	10	-0.08053336068105921
1202	11	-0.04347174216695748
1202	12	0.04355514284560513
1202	13	-0.11919945247347385
1202	14	-0.06740722109745434
1202	15	-0.04553589816560333
1202	16	0.0031835307626693066
1202	17	-0.011789334429941789
1202	18	-0.1066209633550983
1202	19	-0.017463415627069878
1202	20	0.0011596426722846331
1202	21	-0.07406633592849232
1202	22	-0.07117046302798968
1202	23	-0.06242600587230385
1202	24	-0.029791638663283493
1202	25	-0.030762676241394615
1202	26	0.0038778502316034746
1202	27	-0.10293869047887938
1202	28	0.03960206693353996
1202	29	-0.011435577137618225
1202	30	0.02483984181272092
1202	31	0.06260662123381877
1202	32	-0.0014823506699173095
1202	33	-0.09685187291619712
1202	34	-0.022135079834065152
1202	35	-0.05384806843716916
1202	36	-0.33096223460427154
1202	37	-0.17672122517385702
1202	38	-0.25508118878865815
1202	39	1.0597126602402724
1202	40	-0.1971653710307777
1202	41	-0.20808353164774473
1202	42	-0.14769644422065653
1202	43	-0.15352781322908982
1202	44	-0.14277180482554286
1202	45	-0.26902796198898216
1202	46	-0.17990950837749864
1202	47	-0.25375564371854864
1202	48	0.03034160074400205
1202	49	-0.11567625531102266
1202	50	-0.12824907831731025
1202	51	-0.09119826601922654
1202	52	-0.1229432086159008
1202	53	-0.07710345540145624
1202	54	-0.14304725164890186
1202	55	-0.05214618842794749
1202	56	-0.1307876310613807
1202	57	-0.09194542422334706
1202	58	-0.09569094275123291
1202	59	-0.08699958700192667
1202	60	-0.1900901175694898
1202	61	-0.03656055525282653
1203	1	-0.0016052225061358172
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2403	5	0.06419972662137496
2403	6	0.09427645045558108
2403	7	0.0780677732127657
2403	8	-0.009869909754797845
2403	9	-0.028752393533956422
2403	10	-0.02733507218304254
2403	11	0.032881400616282974
2403	12	0.0757120880085651
2403	13	0.09620244871638306
2403	14	0.09725640625208598
2403	15	0.027166830753174054
2403	16	0.025161996877514525
2403	17	0.07238088888442462
2403	18	0.07315841671042733
2403	19	0.002193529535887251
2403	20	-0.019724345946443123
2403	21	0.024308922736793193
2403	22	0.032882498689545514
2403	23	0.09893263354828719
2403	24	0.02342982193336951
2403	25	0.08568520270325308
2403	26	0.08702041791568149
2403	27	0.09210840815881483
2403	28	0.07008199881834543
2403	29	0.03840868480409654
2403	30	0.029180029776163963
2403	31	0.024217459309348044
2403	32	0.02008979538974387
2403	33	0.082535198130536
2403	34	0.009818705301306352
2403	35	0.016493261964626527
2403	36	0.01643009596996592
2403	37	-0.002110300876559279
2403	38	0.055252489431723006
2403	39	1.2632730801402516
2403	40	-0.0032671773766992374
2403	41	0.018253444555043075
2403	42	0.0465380143066767
2403	43	0.06365833955587163
2403	44	0.04599511619532137
2403	45	0.17306981348954764
2403	46	0.04941020191737522
2403	47	0.0495924550915916
2403	48	0.06407047641221908
2403	49	0.06279309285245205
2403	50	0.0542398206307837
2403	51	0.010893150604331936
2403	52	0.017612813974203334
2403	53	0.12036593587290412
2403	54	0.08578907789067358
2403	55	0.04944783640392103
2403	56	0.04535804060615334
2403	57	6.007044195257549E-4
2403	58	-0.055627461978680515
2403	59	0.04537291256696818
2403	60	0.08411502555863848
2403	61	0.13723341784598375
2404	1	-0.008207741107327138
2404	2	-0.08838223945327428
2404	3	0.025386165372125902
2404	4	0.13880395047084448
2404	5	0.03570669297258178
2404	6	-0.13031608371897363
2404	7	0.04183617489520941
2404	8	0.026548991401881494
2404	9	0.01380806913692112
2404	10	0.03644318754356135
2404	11	0.024656505768753334
2404	12	-0.055127160151265286
2404	13	0.03977171790671008
2404	14	0.06010143779782595
2404	15	0.09283126292982757
2404	16	-0.09177083698158128
2404	17	-0.005487801967312578
2404	18	0.09577605603745099
2404	19	-0.07934235374487031
2404	20	-0.07734132807052418
2404	21	0.0017767197925238065
2404	22	-0.12563813748290187
2404	23	0.06295077234955215
2404	24	0.010883817727268763
2404	25	-0.06445280991504632
2404	26	-0.09599088597495609
2404	27	0.06494949648689641
2404	28	0.0018347590707108452
2404	29	-0.0954471248699349
2404	30	-0.1319858482469698
2404	31	-0.0038385343815008355
2404	32	-0.024859599983543035
2404	33	-0.09729195749512343
2404	34	0.04588600288279565
2404	35	0.02747183714864576
2404	36	-0.04968780623823221
2404	37	0.0011822858742291942
2404	38	0.025152594584191945
2404	39	1.2244751043253217
2404	40	-0.05727940135145815
2404	41	-0.013164142195741135
2404	42	-0.06798945677639523
2404	43	-0.05060296433476452
2404	44	-0.10364670820005273
2404	45	0.03476802589754158
2404	46	-0.11086845213099394
2404	47	0.02681051502850096
2404	48	-0.07482936585907396
2404	49	-0.06049160746300686
2404	50	0.0159610351418209
2404	51	-0.11253284834414501
2404	52	0.024832188316999293
2404	53	-0.10268874341205958
2404	54	0.061528431202980016
2404	55	0.04835030748699154
2404	56	-0.040834125534750854
2404	57	-0.04995134605366047
2404	58	-0.03993674168425798
2404	59	-0.10581333874743848
2404	60	0.010859899375667226
2404	61	-0.10328314366821445
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	5.0
17	20.0
18	68.0
19	105.0
20	152.0
21	210.0
22	301.0
23	461.0
24	695.0
25	932.0
26	1351.0
27	1616.0
28	2331.0
29	2941.0
30	3526.0
31	4643.0
32	6430.0
33	7834.0
34	10017.0
35	13005.0
36	19688.0
37	29618.0
38	81580.0
39	580442.0
40	457621.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.082982686748377	12.446709470435945	27.34096882080756	31.129339022008125
2	30.847949278609338	16.34365896916674	25.26895543024299	27.539436321980933
3	28.110741584475097	19.508694573724373	20.31181665676669	32.06874718503385
4	27.469233260960202	20.33358545618325	19.957930569120418	32.239250713736126
5	27.935130177799643	20.362958992096072	19.27295603026453	32.42895479983975
6	29.223567693353342	22.9583556694596	22.222711781154104	25.59536485603296
7	27.60671772766326	22.39805547192257	21.889893300630796	28.10533349978337
8	26.081415282234804	23.98814288266986	22.183710252914306	27.746731582181035
9	25.935689906845095	24.063453365024117	23.02942330773756	26.971433420393232
10	26.573340415619214	22.097955846679934	22.747437362974495	28.581266374726354
11	26.311507980218558	22.766938127094395	22.281703632445684	28.639850260241367
12	27.434719356262644	22.568911539148804	22.184444591312126	27.81192451327643
13	26.412275527030587	21.50509998017286	22.37651487891984	29.706109613876713
14	26.810205345493976	22.318828518113275	22.058464759508254	28.812501376884498
15	27.14057603135788	22.103096215464678	21.966182900848814	28.790144852328623
16	27.037931841973638	22.37920745304518	21.785698841295602	28.797161863685577
17	27.295358246987377	21.637607264401804	21.50526316648349	29.561771322127328
18	28.047320766355554	22.42914246409697	21.678648621524438	27.84488814802304
19	27.005947325090794	21.9270997794537	22.362399263050623	28.704553632404885
20	27.05114993313441	22.025501124761647	21.620635888096622	29.302713054007324
21	27.17141824406634	22.415190034538384	22.46928629651116	27.944105424884118
22	27.8678158246661	21.53757405598759	21.8442011336553	28.750408985691006
23	27.31126891227349	22.51146995780818	21.568905827627933	28.6083553022904
24	27.27928439539064	23.279751108239033	21.716018286657967	27.72494620971236
25	27.210990924393336	21.710225172630718	21.84411954049999	29.23466436247596
26	27.60402515353792	21.88638479495232	22.355790217470236	28.15379983403952
27	27.33272791212091	22.89430504253859	21.64764322250535	28.125323822835153
28	27.237100734093616	22.338492468543798	21.645277021001263	28.779129776361323
29	27.513701530606	23.29876231342705	21.31596704615643	27.87156910981052
30	28.228702350617212	23.135576002800278	21.533820770843175	27.10190087573934
31	27.177782510180787	22.52950204513244	22.58131369875644	27.711401745930335
32	27.605412237178246	22.825766792075346	21.46006055843987	28.108760412306534
33	27.52879626433898	24.269394489035104	21.50909804478322	26.6927112018427
34	27.61536660212648	23.069975105928314	21.501754660805013	27.81290363114019
35	28.5992168688953	23.286115374353475	21.171710347562364	26.942957409188857
36	27.894252006987635	24.19734773289338	21.718221301851433	26.190178958267552
37	27.75391177984861	23.761885062985836	21.090769937491483	27.39343321967407
38	28.088525309788814	24.91699936275746	20.564004526788256	26.430470800665475
39	28.44549437782595	25.318289159409733	20.536005604890622	25.7002108578737
40	28.020639804568077	24.565496049667384	20.34745629258653	27.066407853178013
41	28.19320932805589	25.568928673711422	20.15269343085347	26.08516856737922
42	29.084288177233386	25.405742363084645	20.10879631329487	25.401173146387094
43	28.237269631925116	25.949152777471802	19.932799877283895	25.880777713319187
44	28.391888661243986	25.60213708792397	20.23771349869002	25.768260752142027
45	28.430971782639098	26.05187856001136	19.509820960139297	26.007328697210248
46	28.36161760062272	26.007491883520878	19.96910883139835	25.661781684458052
47	29.36733483301553	26.123843722997762	18.91810739780661	25.590714046180096
48	29.189951313364226	26.749010479008938	18.855443854525934	25.205594353100906
49	29.13153061415984	26.671170608839965	18.77882788168666	25.418470895313533
50	29.235969852960974	26.70960098499257	18.366456074732803	25.687973087313654
51	29.171919226039968	27.33827624668222	18.354217101435793	25.135587425842022
52	28.8003439967428	27.16056635440966	18.339530333479384	25.699559315368152
53	28.843996334835463	27.383315668415207	18.12428758976267	25.64840040698666
54	28.811277479554796	27.827019247009403	18.01976675780622	25.341936515629577
55	28.689214119205968	27.62213883401749	17.9373576709397	25.75128937583684
56	28.382179075761695	27.899229189461757	17.84458625334838	25.874005481428174
57	28.244449829592693	28.02561698704219	17.951391693653605	25.77854148971151
58	28.03859029873702	27.921177748241057	17.726765737075848	26.313466215946075
59	27.91163134906939	28.164325351074947	17.58063239590957	26.34341090394609
60	27.75921533494398	28.364962919990568	17.5637426127597	26.312079132305747
61	27.638375871924858	28.312090555347492	17.498386495353678	26.551147077373972
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	44.0
1	35.5
2	26.5
3	26.0
4	35.0
5	44.0
6	38.0
7	33.0
8	34.0
9	35.0
10	36.0
11	39.5
12	51.0
13	59.0
14	57.0
15	55.0
16	67.5
17	91.0
18	102.0
19	126.0
20	191.0
21	232.0
22	308.5
23	385.0
24	535.0
25	950.5
26	1216.0
27	1564.5
28	1913.0
29	2560.5
30	3975.0
31	4742.0
32	5440.5
33	6139.0
34	7372.0
35	9858.5
36	11112.0
37	12921.0
38	14730.0
39	17030.5
40	22273.0
41	25215.0
42	28049.0
43	34173.0
44	37463.0
45	40866.0
46	44269.0
47	47684.0
48	54989.5
49	58880.0
50	62781.0
51	66682.0
52	70633.0
53	79143.0
54	83702.0
55	87133.5
56	90565.0
57	91902.0
58	92333.5
59	91428.0
60	89280.0
61	85752.5
62	84373.0
63	74612.0
64	64851.0
65	57961.0
66	44732.0
67	38393.0
68	33122.5
69	27852.0
70	23505.0
71	15917.0
72	12676.0
73	10349.0
74	8022.0
75	6456.0
76	3854.0
77	2818.0
78	2196.0
79	1574.0
80	1185.0
81	616.5
82	437.0
83	334.0
84	179.5
85	128.0
86	92.0
87	56.0
88	45.5
89	29.0
90	23.0
91	13.5
92	4.0
93	8.5
94	10.0
95	7.0
96	7.5
97	8.0
98	5.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03182133057222095
3	8.159315531338707E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.2217873307044016
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1225593.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.43527108849155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.5983759731113	63.1342925897391
2	13.00125927705206	19.8750955466648
3	2.5934146377999077	5.946850526552942
4	0.6737844713120454	2.0600359487980864
5	0.28063619487964897	1.0725251816434356
6	0.16272811275707588	0.7462900447383429
7	0.11969420021548118	0.6404201048833341
8	0.09107214827052057	0.556889947333483
9	0.07322252308748534	0.5037105061777746
>10	0.4041852357496772	4.69985674267483
>50	0.0012785345294872782	0.06592004450105363
>100	2.324608235431415E-4	0.021499529338618564
>500	0.0	0.0
>1k	0.0	0.0
>5k	1.1623041177157076E-4	0.6766132869542107
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAACAGATCTGGGGGGGGGGGGGGG	7616	0.6214134708667559	TruSeq Adapter, Index 6 (97% over 38bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.7129609911283762	0.0	0.0	0.0	0.0
2	0.714184888458077	0.0	0.0	0.0	0.0
3	0.7146744473899573	0.0	0.0	0.0	0.0
4	0.7167958694281055	0.0	0.0	0.0	0.0
5	0.7178565804471795	0.0	0.0	0.0	0.0
6	0.7224257971447291	0.0	0.0	0.0	0.0
7	0.7256079302019512	0.0	0.0	0.0	0.0
8	0.7634671542673628	0.0	2.447794659401612E-4	0.0	0.0
9	0.7658333557714511	0.0	2.447794659401612E-4	0.0	0.0
10	0.7699130135371204	0.0	2.447794659401612E-4	0.0	0.0
11	0.7752165686324906	0.0	2.447794659401612E-4	0.0	0.0
12	0.7890058118804529	0.0	2.447794659401612E-4	0.0	0.0
13	0.8071194923600249	0.0	2.447794659401612E-4	0.0	2.447794659401612E-4
14	0.8321685910412348	0.0	2.447794659401612E-4	0.0	2.447794659401612E-4
15	0.8647242600112762	0.0	2.447794659401612E-4	8.159315531338707E-5	3.263726212535483E-4
16	0.9019307388341806	0.0	2.447794659401612E-4	8.159315531338707E-5	3.263726212535483E-4
17	0.9491731757606318	0.0	2.447794659401612E-4	8.159315531338707E-5	3.263726212535483E-4
18	1.0118367190413131	0.0	2.447794659401612E-4	8.159315531338707E-5	3.263726212535483E-4
19	1.094898551150341	0.0	2.447794659401612E-4	8.159315531338707E-5	3.263726212535483E-4
20	1.1955029116517473	0.0	2.447794659401612E-4	8.159315531338707E-5	5.711520871937095E-4
21	1.3358431387907732	0.0	2.447794659401612E-4	8.159315531338707E-5	5.711520871937095E-4
22	1.500416533057875	0.0	2.447794659401612E-4	8.159315531338707E-5	5.711520871937095E-4
23	1.7004829498862999	0.0	2.447794659401612E-4	8.159315531338707E-5	5.711520871937095E-4
24	1.9637840620826	0.0	2.447794659401612E-4	8.159315531338707E-5	6.527452425070966E-4
25	2.263720501014611	0.0	2.447794659401612E-4	8.159315531338707E-5	6.527452425070966E-4
26	2.626320483227303	0.0	2.447794659401612E-4	8.159315531338707E-5	6.527452425070966E-4
27	3.051502415565363	0.0	2.447794659401612E-4	8.159315531338707E-5	6.527452425070966E-4
28	3.5406533816691184	0.0	2.447794659401612E-4	8.159315531338707E-5	6.527452425070966E-4
29	4.093202229451376	0.0	2.447794659401612E-4	8.159315531338707E-5	6.527452425070966E-4
30	4.722775015849471	0.0	2.447794659401612E-4	8.159315531338707E-5	6.527452425070966E-4
31	5.431166790280297	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	7.343383978204837E-4
32	6.234369811185279	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	7.343383978204837E-4
33	7.152945553703391	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	7.343383978204837E-4
34	8.166495729006286	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	7.343383978204837E-4
35	9.218068314685217	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	7.343383978204837E-4
36	10.393825682751125	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	7.343383978204837E-4
37	11.623026567547301	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	7.343383978204837E-4
38	12.958543333716822	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	7.343383978204837E-4
39	14.355418152682008	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	7.343383978204837E-4
40	15.871255792094113	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	7.343383978204837E-4
41	17.454326191484448	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	8.159315531338707E-4
42	19.133513327833956	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	8.159315531338707E-4
43	20.854068193927347	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	8.159315531338707E-4
44	22.65368682751941	2.447794659401612E-4	2.447794659401612E-4	8.159315531338707E-5	8.159315531338707E-4
45	24.474846054114213	3.263726212535483E-4	2.447794659401612E-4	8.159315531338707E-5	8.159315531338707E-4
46	26.37302921932485	3.263726212535483E-4	2.447794659401612E-4	8.159315531338707E-5	8.159315531338707E-4
47	28.239880612895146	3.263726212535483E-4	2.447794659401612E-4	8.159315531338707E-5	8.159315531338707E-4
48	30.1582988806235	3.263726212535483E-4	2.447794659401612E-4	8.159315531338707E-5	8.159315531338707E-4
49	32.110659900962226	3.263726212535483E-4	2.447794659401612E-4	8.159315531338707E-5	8.159315531338707E-4
50	34.06400003916472	3.263726212535483E-4	2.447794659401612E-4	8.159315531338707E-5	8.159315531338707E-4
>>END_MODULE